Genetics and Genomics Commons^™

Life History Response Of Mediterranean Fruit Flies To Dietary Restriction, James R. Carey, Pablo Liedo, Lawrence G. Harshman, Ying Zhang, Hans-Georg Müller, Linda Partridge, Jane-Ling Wang

Lawrence G. Harshman Publications

The purpose of this study was to investigate medfly longevity and reproduction across a broad spectrum of diet restriction using a protocol similar to those applied in most rodent studies. Age-specific reproduction and age of death were monitored for 1,200 adult males and 1,200 females, each individually maintained on one of 12 diets from ad libitum to 30% of ad libitum. Diet was provided in a fixed volume of solution that was fully consumed each day, ensuring control of total nutrient consumption for every fly. Contrary to expectation and precedence, increased longevity was not observed at any level of …

Functional Requirement Of Aquaporin-5 In Plasma Membranes Of Sweat Glands, Lene N. Nejsum, Tae-Hwan Kwon, Uffe B. Jensen, Ornella Fumagalli, Jørgen Frøkiaer, Carissa M. Krane, Anil G. Menon, Landon S. King, Peter C. Agre, Søren Nielsen

Biology Faculty Publications

The distribution and function of aquaporins (AQPs) have not previously been defined in sweat glands. In this study, AQP1, AQP3, and AQP5 mRNA were demonstrated in rat paw by reverse transcription (RT)–PCR, but AQP2 and AQP4 were not. AQP1, AQP3, and AQP5 protein were confirmed in these tissues by immunoblotting. AQP1 was identified in capillary endothelial cells by immunohistochemical labeling, but not in sweat glands or epidermis. Abundant AQP3 expression was seen in basal levels of epidermis, but not in sweat glands. AQP2 and AQP4 were not observed in either skin or sweat glands. Immunohistochemical labeling revealed abundant AQP5 in …

Human Genomics Of Biotechnology, Brian Bluelake, Bob Wang, Serin Hudson, Fatih Ersan, Aaron Freier

Engineering and Technology Management Student Projects

Biotechnology is the term for the techniques of managing biological systems for human benefit. A biological system can range from a single cell organism to different types of plants, animals, and to the very complicated human being. Biotechnology includes a great range of scientific and technological disciplines and has a great variety of important applications in biology, medicine, agriculture, and various industries. In our report, we shall only focus on one of the most active frontiers of today's biotechnology-human genomics.

Registration Of N547 Maize Germplasm Line, Nora E. D'Croz-Mason, John E. Foster, Stephen C. Mason

Department of Entomology: Faculty Publications

N547 germplasm line (Reg. no. GP-367, PI 615526) was released by the Agriculture Research Division, Institute of Agriculture and Natural Resources, University of Nebraska, on 24 Aug. 1999. N547 is a yellow endosperm maize inbred germplasm with resistance to stalk and shank tunneling damage caused by the second generation of the European corn borer (ECB) Ostrinia nubilalis (Hubner).

N547 was derived by selfing a selected full-sib family from MBITA, a tropical population developed by CIMMYT, Mexico. MBITA was created to recombine sources of resistance to temperate and subtropical stalk borers. These insects included ECB, southwestern corn borer (SWCB) Diatraea grandiosella …

Small Glutamine-Rich Protein/Viral Protein U–Binding Protein Is A Novel Cochaperone That Affects Heat Shock Protein 70 Activity, Peter C. Angeletti, Doriann Walker, Antonito T. Panganiban

Nebraska Center for Virology: Faculty Publications

Molecular chaperone complexes containing heat shock protein (Hsp) 70 and Hsp90 are regulated by cochaperones, including a subclass of regulators, such as Hsp70 interacting protein (Hip), C-terminus of Hsp70 interacting protein (CHIP), and Hsp70-Hsp90 organizing factor (Hop), that contain tetratricopeptide repeats (TPRs), where Hsp70 refers to Hsp70 and its nearly identical constitutive counterpart, Hsc70, together. These proteins interact with the Hsp70 to regulate adenosine triphosphatase (ATPase) and folding activities or to generate the chaperone complex. Here we provide evidence that small glutamine-rich protein/viral protein U–binding protein (SGT/UBP) is a cochaperone that negatively regulates Hsp70. By “Far-Western” and pull-down assays, SGT/UBP …

Cases And Guidelines In Genetics, Roger B. Dworkin

Articles by Maurer Faculty

No abstract provided.

An Equity Paradigm For Preventing Genetic Discrimination, Anita Silvers, Michael Ashley Stein

Faculty Publications

No abstract provided.

Application Of Fuzzy-Neural Network In Classification Of Soils Using Ground-Penetrating Radar Imagery, Lameck O. Odhiambo, Robert S. Freeland, Ronald E. Yoder, J. Wesley Hines

Department of Animal Science: Faculty Publications

Errors associated with visual inspection and interpretation of radargrams often inhibits the intensive surveying of widespread areas using ground-penetrating radar (GPR). To automate the interpretive process, this paper presents an application of a fuzzy-neural network (F-NN) classifier for unsupervised clustering and classification of soil profile using GPR imagery. The classifier clusters and classifies soil profiles strips along a traverse based on common pattern similarities that can relate to physical features of the soil (e.g., number of horizons; depth, texture and structure of the horizons; and relative arrangement of the horizons, etc). This paper illustrates this classification procedure by its application …

Rapid Communication: Physical And Linkage Mapping Of The Porcine Calcitonin (Calc) Gene, J. E. Neil, S. E. Zhang, J. M. Helm, Daniel C. Ciobanu, M. F. Rothschild

Department of Animal Science: Faculty Publications

Genus and Species. Sus scrofa.

Locus. Porcine calcitonin (CALC) gene.

Source and Description of Primers. Primers, CCA1F and CCA1R, were designed from canine calcitonin sequence (GenBank Accession no. AJ271090) to amplify genomic porcine DNA. Using sequence obtained from the amplified PCR product, additional pig-specific primers (CIPBF, CIPBR, CIPCF, CIPCR) were designed.

Primer Sequences. CCA1F: 5′-CAC TTT GGA TTG GCC GCG C-3′; CCA1R: 5′-ACC AGG GCA GCC AGC AGG A-3′; CIPBF: 5′-AAC TTC CCA CTC TGC ACA CT-3′; CIPBR: 5′-AGA CCA AAC TTC AGC AGG AT- 3′; CIPCF: 5′-TTC TCC TTC CTC TGC TTC TG-3′; CIPCR: 5′-GCA AAC CCA ATA …

Rapid Communication: Mapping Of The Ca2+ Atpase Of Fast Twitch 1 Skeletal Muscle Sarcoplasmic Reticulum (Atp2a1) Gene To Porcine Chromosome 3, Daniel C. Ciobanu, Y. Ciobanu, M. F. Rothschild

Department of Animal Science: Faculty Publications

Locus Name. Ca²⁺ ATPase of fast twitch 1 skeletal muscle sarcoplasmic reticulum.

Species. Sus scrofa.

Locus Symbol. ATP2A1.

Source of Primers. Two sets of PCR primers were designed based on human ATP2A1 sequences (Gen- Bank accession numbers U96773 and U96777) available in the GenBank database. Using pig genomic DNA as a template we used set A primers to amplify approximately 1,100 bp spanning exon 1 to exon 2 and set B primers to amplify 1,200 bp gene segment covering some of exon 8 through part of exon 9. These sequences were deposited in GenBank, accession number AY027797-AY027799.

Methods And Compositions To Enhance Tenderness And Value Of Meat, Chris R. Calkins, Jesus Pablo Velazco De La Garcia, Nancy Coromoto Jerez-Timaure

Department of Animal Science: Faculty Publications

A method of enhancing the tenderness of meat for animal or human consumption by treatment of pre-rigor muscle with the compositions of the invention. It has been found that other quality meat traits can also be enhanced with the method of the invention.

Il-12 Plasmid Delivery By In Vivo Electroporation For The Successful Treatment Of Established Subcutaneous B16.F10 Melanoma, M. Lee Lucus, Loree Heller, Domenico Coppola, Richard Heller

Bioelectrics Publications

Interleukin-12 (IL-12) has been used in numerous immunotherapy protocols against melanoma. However, delivery of IL-12 in the form of recombinant protein can result in severe toxicity, and gene therapy has had limited success against B16.F10 murine melanoma. The purpose of this study was to examine the effectiveness of in vivo electroporation for the delivery of plasmid DNA encoding IL-12 as an antitumor agent against B16.F10 melanoma. We treated mice bearing established B16.F10 melanoma tumors with intratumoral (i.t.) or intramuscular (i.m.) injections of a plasmid encoding IL-12, followed by in vivo electroporation. For i.t. treatments, we used an applicator containing six …

Variability Of Placental Expression Of Cyclin E Low Molecular Weight Variants, A Bukovsky, Maria Cekanova Ms, Rndr, Phd, Mr Caudle, J Wimalasena, Js Foster, Ja Keenan, Rf Elder

Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology

No abstract provided.