Genetics and Genomics Commons^™

Molecular Cloning Of Spinach Chloroplast Dna Isolated By Alkaline Lysis, Robert Gray Drager

Dissertations and Theses

Chloroplast genomes of land plants show conservation of structure and gene arrangement. The spinach chloroplast genome is comprised of a covalently closed. circular DNA molecule of 150 kilobases and is typical of these plants. Approximately 20% of the proteins found in the spinach chloroplast are encoded by the chloroplast genome and translated on chloroplast ribosomes. The remainder are encoded on chromosomes in the nucleus, translated on cytoplasmic ribosomes and transported into the chloroplast.

Spinach chloroplast DNA was isolated from crude 2 chloroplast preparations by a new method. Chloroplasts were lysed with alkaline sodium dodecyl sulfate, contaminating macromolecules precipitated with acidified …

Restriction Mapping And Expression Of Recombinant Plasmids Containing The Arsenic Resistance Genes Of The Plasmid R45, Terry M. Coons

Dissertations and Theses

The trivalent (arsenite) and pentavalent (arsenate) forms of arsenic are introduced into the environment through the use of arsenic in herbicides, pesticides, fertilizers, and the smelting of arsenic-bearing ores. Bacteria resistant to arsenic are readily isolated from surface waters, sewage, and clinical infections. Although some bacterial resistance is provided by inducible phosphate transport systems that discriminate against arsenate, marked resistance is carried on bacterial plasmids.

A 6.9 kilobase fragment previously derived from one such plasmid, R45, and containing the genes for inducible resistance to arsenite and arsenate was ligated into the cloning vectors puce and pUC9 in opposite orientations and …

Audiological Characteristics Of The Monge Family Of Costa Rica, Christine Moulton

Dissertations and Theses

The audiological characteristics of the Monge family of Costa Rica were investigated in a sample of fifty-two affected members and twelve unaffected members. Through laboratory analysis by staff personnel from the University of Costa Rica and audiological test results obtained in the present investigation, it was concluded that affected Monge members demonstrate a slowly progressive low frequency sensorineural hearing loss of autosomal dominant transmission. The initial site of lesion appears to be the apical portion of the cochlea, with significant onset occurring during early childhood following normal speech and language acquisition. The rate at which the hearing loss progresses and …

Origin Of Human Trisomy 21 Mosaicism, Diane Dusenbery Waggoner

Dissertations and Theses

Down Syndrome is a human condition caused by an extra copy of a #21 chromosome. At least one to two percent of free (not translocated) trisomy 21 cases are mosaics, i.e., they have two or more distinct cell lines. Usually, one cell line is 47 ,XX or XY ,+21 while the other cell line is normal 46,XX or 46,XY.

The purpose of the study was to establish the etiologies of the separate cell lines by determining whether the zygote was trisomic or normal. Meiotic non-disjunction in the formation of a gamete could lead to a trisomic zygote; loss of a …

Cytologic Characterization Of Human Constitutive Heterochromatin, Timothy Atchison Donlon

Dissertations and Theses

A study was conducted to cytologically map certain subsets of constitutive heterochromatin onto specific portions of human chromosomes. This involved sequentially staining metaphase chromosomes from lymphocytes first with the Centromeric Dot, Giemsa-11, G-banding or Lateral Asymmetry staining techniques, which are cytochemical methods which stain particular chromosomes or chromosomal regions. Then those same metaphase chromosomes were stained using the C-banding technique, a method which is believed to denote constitutive heterochromatin. With the exception of the G-banding technique, areas depicted by the special staining techniques were found to reside only within the areas stained by the C-band technique and are thus believed …

An Analysis Of Staggered Spondaic Word Test Performances Of Dyslexic Children And Their Parents, Nancy Jane Maxwell

Dissertations and Theses

The purpose of this study was to investigate the possibility of a familial lineage for dyslexia by analyzing certain auditory processing characteristics of dyslexic children and their parents. The Staggered Spondaic Word test was administered to twenty-one dyslexic children, eight to thirteen years of age, their natural parents and normal reading siblings.

The experimental test data were tabulated and analyzed according to listening condition for each of the four groups: dyslexic children, normal reading siblings, affected parents and non-affected parents. A statistical analysis of the experimental data revealed significantly poorer Staggered Spondaic Word test performances for dyslexic children and their …

A Test Of The Simple Recessive Hypothesis For The Inability To Taste Phenyl-Thio-Urea: A Family Study, Susan I. Wolf

Dissertations and Theses

This thesis is a report on the analysis of family data gathered to test the simple recessive hypothesis for the inability to taste phenyl-thio-urea (P.T.C.). The simple recessive hypothesis states that the inability of a minority of persons to taste high concentrations of P.T.C. is due to the action of an autosomal recessive gene in the homozygous condition.