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Articles 151 - 180 of 186
Full-Text Articles in Genetics and Genomics
Genetic And Phylogenetic Divergence Of Feline Immunodeficiency Virus In The Puma (Puma Concolor), Margaret A. Carpenter, Eric W. Brown, Melanie Culver, Warren E. Johnson, Jill Pecon-Slattery, Dulce Brousset, Stephen J. O'Brien
Genetic And Phylogenetic Divergence Of Feline Immunodeficiency Virus In The Puma (Puma Concolor), Margaret A. Carpenter, Eric W. Brown, Melanie Culver, Warren E. Johnson, Jill Pecon-Slattery, Dulce Brousset, Stephen J. O'Brien
Biology Faculty Articles
Feline immunodeficiency virus (FIV) is a lentivirus which causes an AIDS-like disease in domestic cats (Felis catus). A number of other felid species, including the puma (Puma concolor), carry a virus closely related to domestic cat FIV. Serological testing revealed the presence of antibodies to FIV in 22% of 434 samples from throughout the geographic range of the puma. FIV-Pco pol gene sequences isolated from pumas revealed extensive sequence diversity, greater than has been documented in the domestic cat. The puma sequences formed two highly divergent groups, analogous to the clades which have been defined for …
Prevalence Of Antibodies To Feline Parvovirus, Calicivirus, Herpesvirus, Coronavirus, And Immunodeficiency Virus And Of Feline Leukemia Virus Antigen And The Interrelationship Of These Viral Infections In Free-Ranging Lions In East Africa, Regina Hofmann-Lehmann, Daniela Fehr, Markus Grob, Muhamed Elgizoli, Craig Packer, Janice S. Martenson, Stephen J. O'Brien, Hans Lutz
Prevalence Of Antibodies To Feline Parvovirus, Calicivirus, Herpesvirus, Coronavirus, And Immunodeficiency Virus And Of Feline Leukemia Virus Antigen And The Interrelationship Of These Viral Infections In Free-Ranging Lions In East Africa, Regina Hofmann-Lehmann, Daniela Fehr, Markus Grob, Muhamed Elgizoli, Craig Packer, Janice S. Martenson, Stephen J. O'Brien, Hans Lutz
Biology Faculty Articles
While viral infections and their impact are well studied in domestic cats, only limited information is available on their occurrence in free-ranging lions. The goals of the present study were (i) to investigate the prevalence of antibodies to feline calicivirus (FCV), herpesvirus (FHV), coronavirus (FCoV), parvovirus (FPV), and immunodeficiency virus (FIV) and of feline leukemia virus (FeLV) antigen in 311 serum samples collected between 1984 and 1991 from lions inhabiting Tanzania’s national parks and (ii) to evaluate the possible biological importance and the interrelationship of these viral infections. Antibodies to FCV, never reported previously in free-ranging lions, were detected in …
Evidence Of Natural Bluetongue Virus Infection Among African Carnivores, Kathleen A. Alexander, N. James Maclachlan, Pieter W. Kat, Carol House, Stephen J. O'Brien, Nicholas W. Lerche, Mary Sawyer, Laurence G. Frank, Kay Holekamp, Laura Smale, J. Weldon Mcnutt, M. Karen Laurenson, M. G. L. Mills, Bennie I. Osburn
Evidence Of Natural Bluetongue Virus Infection Among African Carnivores, Kathleen A. Alexander, N. James Maclachlan, Pieter W. Kat, Carol House, Stephen J. O'Brien, Nicholas W. Lerche, Mary Sawyer, Laurence G. Frank, Kay Holekamp, Laura Smale, J. Weldon Mcnutt, M. Karen Laurenson, M. G. L. Mills, Bennie I. Osburn
Biology Faculty Articles
Bluetongue is an International Office of Epizootics List A disease described as the century's most economically devastating affliction of sheep. Bluetongue (BLU) viruses were thought to infect only ruminants, shrews, and some rodents, but recently, inadvertent administration of BLU virus-contaminated vaccine resulted in mortality and abortion among domestic dogs. We present evidence of natural BLU virus infection among African carnivores that dramatically widens the spectrum of susceptible hosts. We hypothesize that such infection occurred after ingestion of meat and organs from BLU virus infected prey species. The effect of BLU virus on endangered carnivores such as the cheetah and African …
A Lion Lentivirus Related To Feline Immunodeficiency Virus: Epidemiologic And Phylogenetic Aspects, Eric W. Brown, Naoya Yuhki, Craig Packer, Stephen J. O'Brien
A Lion Lentivirus Related To Feline Immunodeficiency Virus: Epidemiologic And Phylogenetic Aspects, Eric W. Brown, Naoya Yuhki, Craig Packer, Stephen J. O'Brien
Biology Faculty Articles
Feline immunodeficiency virus (FIV) is a novel lentivirus that is genetically homologous and functionally analogous to the human AIDS viruses, human immunodeficiency virus types 1 and 2. FIV causes immunosuppression in domestic cats by destroying the CD4 T-lymphocyte subsets in infected hosts. A serological survey of over 400 free-ranging African and Asian lions (Panthera leo) for antibodies to FIV revealed endemic lentivirus prevalence with an incidence of seropositivity as high as 90%o. A lion lentivirus (FIV-Ple) was isolated by infection of lion lymphocytes in vitro. Seroconversion was documented in two Serengeti lions, and discordance of mother-cub serological status …
Phylogenetic Associations Of Human And Simian T-Cell Leukemia/Lymphotropic Virus Type I Strains: Evidence For Interspecies Transmission, Igor J. Koralnik, Enzo Boeri, W. Carl Saxinger, Anita Lo Monico, Jake Fullen, Antoine Gessain, Hong-Guang Guo, Robert C. Gallo, Phillip Markham, Vaniambadi Kalyanaraman, Vanessa Hirsch, Jonathan Allan, Krishna Murthy, Patricia Alford, Jill Pecon-Slattery, Stephen J. O'Brien, Genoveffa Ranchini
Phylogenetic Associations Of Human And Simian T-Cell Leukemia/Lymphotropic Virus Type I Strains: Evidence For Interspecies Transmission, Igor J. Koralnik, Enzo Boeri, W. Carl Saxinger, Anita Lo Monico, Jake Fullen, Antoine Gessain, Hong-Guang Guo, Robert C. Gallo, Phillip Markham, Vaniambadi Kalyanaraman, Vanessa Hirsch, Jonathan Allan, Krishna Murthy, Patricia Alford, Jill Pecon-Slattery, Stephen J. O'Brien, Genoveffa Ranchini
Biology Faculty Articles
Homologous env sequences from 17 human T-leukemia/lymphotropic virus type I (HTLV-I) strains from throughout the world and from 25 simian T-leukemia/lymphotropic virus type I (STLV-I) strains from 12 simian species in Asia and Africa were analyzed in a phylogenetic context as an approach to resolving the natural history of these related retroviruses. STLV-I exhibited greater overall sequence variation between strains (1 to 18% compared with 0 to 9% for HTLV-I), supporting the simian origin of the modern viruses in all species. Three HTLV-I phylogenetic clusters or clades (cosmopolitan, Zaire, and Melanesia) were resolved with phenetic, parsimony, and likelihood analytical procedures. …
Editorial Overview, Stephen J. O'Brien, Michael T. Clegg
Editorial Overview, Stephen J. O'Brien, Michael T. Clegg
Biology Faculty Articles
No abstract provided.
Worldwide Prevalence Of Lentivirus Infection In Wild Feline Species: Epidemiologic And Phylogenetic Aspects, Robert A. Olmstead, Raymond Langley, Melody E. Roelke, Robert M. Goeken, Diane Adger-Johnson, Julie P. Goff, John P. Albert, Craig Packer, M. Karen Laurenson, Tim M. Caro, Lue Scheepers, David E. Wildt, Mitchell Bush, Janice S. Martenson, Stephen J. O'Brien
Worldwide Prevalence Of Lentivirus Infection In Wild Feline Species: Epidemiologic And Phylogenetic Aspects, Robert A. Olmstead, Raymond Langley, Melody E. Roelke, Robert M. Goeken, Diane Adger-Johnson, Julie P. Goff, John P. Albert, Craig Packer, M. Karen Laurenson, Tim M. Caro, Lue Scheepers, David E. Wildt, Mitchell Bush, Janice S. Martenson, Stephen J. O'Brien
Biology Faculty Articles
The natural occurrence of lentiviruses closely related to feline immunodeficiency virus (FIV) in nondomestic felid species is shown here to be worldwide. Cross-reactive antibodies to FIV were common in several free-ranging populations of large cats, including East African lions and cheetahs of the Serengeti ecosystem and in puma (also called cougar or mountain lion) populations throughout North America. Infectious puma lentivirus (PLV) was isolated from several Florida panthers, a severely endangered relict puma subspecies inhabiting the Big Cypress Swamp and Everglades ecosystems in southern Florida. Phylogenetic analysis of PLV genomic sequences from disparate geographic isolates revealed appreciable divergence from domestic …
Response [3], Stephen J. O'Brien, Ernst Mayr
Response [3], Stephen J. O'Brien, Ernst Mayr
Biology Faculty Articles
No abstract provided.
Application Of Dna Fingerprints For Cell-Line Individualization, Dennis A. Gilbert, Yvonne Reid, Mitchell H. Gail, David Pee, Christine White, Robert J. Hay, Stephen J. O'Brien
Application Of Dna Fingerprints For Cell-Line Individualization, Dennis A. Gilbert, Yvonne Reid, Mitchell H. Gail, David Pee, Christine White, Robert J. Hay, Stephen J. O'Brien
Biology Faculty Articles
DNA fingerprints of 46 human cell lines were derived using minisatellite probes for hypervariable genetic loci. The incidence of 121 HaeIII DNA fragments among 33 cell lines derived from unrelated individuals was used to estimate allelic and genotypic frequencies for each fragment and for composite individual DNA fingerprints. We present a quantitative estimate of the extent of genetic difference between individuals, an estimate based on the percentage of restriction fragments at which they differ. The average percent difference (APD) among pairwise combinations from the population of 33 unrelated cell lines was 76.9%, compared with the APD in band sharing …
Dna Recombination And Natural Selection Pressure Sustain Genetic Sequence Diversity Of The Feline Mhc Class I Genes, Naoya Yuhki, Stephen J. O'Brien
Dna Recombination And Natural Selection Pressure Sustain Genetic Sequence Diversity Of The Feline Mhc Class I Genes, Naoya Yuhki, Stephen J. O'Brien
Biology Faculty Articles
Sequence comparisons of seven distinct MHC class I cDNA clones revealed that feline class I molecules have a remarkable similarity to human HLA genes in their organization of functional domains as well as in the nonrandom partitioning of genetic variability according to the functional constraints ascribed to different regions of the MHC molecule. The distribution of the pattern of sequence polymorphism in the cat as compared with genetic diversity of human and mouse class I genes provides evidence for four coordinate factors that contribute to the origin and sustenance of abundant allele diversity that characterizes the MHC in the species. …
Prevalence And Implications Of Feline Coronavirus Infections Of Captive And Free-Ranging Cheetahs (Acinonyx Jubatus), J. L. Heeney, J. F. Evermann, A. J. Mckeirnan, L. Marker-Kraus, M. E. Roelke, M. Bush, D. E. Wildt, D. G. Meltzer, L. Colly, J. Lukas, V. J. Manton, T. Caro, Stephen J. O'Brien
Prevalence And Implications Of Feline Coronavirus Infections Of Captive And Free-Ranging Cheetahs (Acinonyx Jubatus), J. L. Heeney, J. F. Evermann, A. J. Mckeirnan, L. Marker-Kraus, M. E. Roelke, M. Bush, D. E. Wildt, D. G. Meltzer, L. Colly, J. Lukas, V. J. Manton, T. Caro, Stephen J. O'Brien
Biology Faculty Articles
The extent and progression of exposure to feline infectious peritonitis (FIP) virus in the cheetah, Acinonyx jubatus, was monitored by a world-wide serological survey with indirect fluorescent antibody titers to coronavirus. The indirect fluorescent antibody assay was validated by Western blots, which showed that all indirect fluorescent antibody-positive cheetah sera detected both domestic cat and cheetah coronavirus structural proteins. There was a poor correlation between indirect fluorescent antibody results and the presence of coronaviruslike particles in cheetah feces, suggesting that electron microscopic detection of shed particles may not be an easily interpreted diagnostic parameter for FIP disease. Low, but …
Chromosomal Localization Of Nucleic Acid-Binding Proteins By Affinity Mapping: Assignment Of The Ire-Binding Protein Gene To Human Chromosome 9, Matthias W. Hentze, Hector Seuanez, Stephen J. O'Brien, Joe B. Harford, Richard D. Klausner
Chromosomal Localization Of Nucleic Acid-Binding Proteins By Affinity Mapping: Assignment Of The Ire-Binding Protein Gene To Human Chromosome 9, Matthias W. Hentze, Hector Seuanez, Stephen J. O'Brien, Joe B. Harford, Richard D. Klausner
Biology Faculty Articles
Three human mRNAs are regulated post-transcriptionally by iron via iron-responsive elements (IREs) contained in each mRNA. A cytoplasmic protein (IRE-BP) binds to these cis-acting elements and mediates the translational regulation of ferritin H- and L-chain mRNA and the iron-dependent stability of transferrin receptor (TfR) mRNA. We have taken advantage of the different mobilities of the human and rodent IRE/IRE-BP complexes on non-denaturing polyacrylamide gels to determine the chromosomal localization of the gene encoding the IRE-BP. Utilizing a panel of 34 different human/rodent hybrid cell lines we have assigned the IRE-BP gene to human chromosome 9. This new technique based on …
The T(11;14)(P15;Q11) In A T-Cell Acute Lymphoblastic Leukemia Cell Line Activates Multiple Transcripts, Including Ttg-1, A Gene Encoding A Potential Zinc Finger Protein, Elizabeth A. Mcguire, Richard D. Hockett, Karen M. Pollock, Marty F. Bartholdi, Stephen J. O'Brien, Stanley J. Korsmeyer
The T(11;14)(P15;Q11) In A T-Cell Acute Lymphoblastic Leukemia Cell Line Activates Multiple Transcripts, Including Ttg-1, A Gene Encoding A Potential Zinc Finger Protein, Elizabeth A. Mcguire, Richard D. Hockett, Karen M. Pollock, Marty F. Bartholdi, Stephen J. O'Brien, Stanley J. Korsmeyer
Biology Faculty Articles
Interchromosomal translocations within lymphoid neoplasms frequently involve the antigen receptor genes. We cloned the breakpoints of the t(11;14)(p15;q11) in a CD3-negative T-cell acute lymphoblastic leukemia cell line (RPMI 8402) in order to identify new genes potentially involved in T-cell neoplasia. An extensive comparison of both breakpoints and their germ line counterparts indicated that an inadvertant recombinase-mediated break at chromosome segment 11p15 recombined with the delta T-cell receptor at 14q11. The derivative 11 breakpoint resembles a coding joint in which 11p15 rather than a variable region was introduced 5' to a Dδ1Dδ2Jδ1 intermediate rearrangement. Conversely, the …
In Vitro Maturation And Fertilization Of Domestic Cat Follicular Oocytes, L. A. Johnston, S. J. O'Brien, D. E. Wildt
In Vitro Maturation And Fertilization Of Domestic Cat Follicular Oocytes, L. A. Johnston, S. J. O'Brien, D. E. Wildt
Biology Faculty Articles
No abstract provided.
The Gli-Kruppel Family Of Human Genes, John M. Ruppert, Kenneth W. Kinzler, Albert J. Wong, Sandra H. Bigner, Fa-Ten Kao, Martha L. Law, Hector Seuanez, Stephen J. O'Brien, Bert Vogelstein
The Gli-Kruppel Family Of Human Genes, John M. Ruppert, Kenneth W. Kinzler, Albert J. Wong, Sandra H. Bigner, Fa-Ten Kao, Martha L. Law, Hector Seuanez, Stephen J. O'Brien, Bert Vogelstein
Biology Faculty Articles
Previous characterization of GLI, a gene found to be amplified and expressed in a subset of human brain tumors, revealed the presence of five tandem zinc fingers related to those of Krüppel (Kr), a Drosophila segmentation gene of the gap class. We have used the GLI cDNA as a molecular probe to isolate related sequences from the human genome. Partial characterization of six related loci, including sequence determination, expression studies, and chromosome localization, revealed that each locus could encode a separate finger protein. The predicted proteins all had similar H-C links, i.e., a conserved stretch of 9 …
The Κ-Deleting Element: Germline And Rearranged, Duplicated And Dispersed Forms, Winfried B. Graninger, Paula L. Goldman, Cynthia C. Morton, Stephen J. O'Brien, Stanley J. Korsmeyer
The Κ-Deleting Element: Germline And Rearranged, Duplicated And Dispersed Forms, Winfried B. Graninger, Paula L. Goldman, Cynthia C. Morton, Stephen J. O'Brien, Stanley J. Korsmeyer
Biology Faculty Articles
Human light chain genes are used in a κ before λ order. Accompanying this hierarchy is the rearrangement of a κ-deleting element (Kde) which eliminates the kappa locus before λ gene rearrangement. In approximately 60% of rearrangements the Kde recombines at a conserved heptamer within the Jκ-Cκ intron. We demonstrated that aberrant V/J rearrangements possessing apparent "N" nucleotides existed 5' to the Jκ-Kde rearrangements. This suggests that the Kde may selectively eliminate nonfunctional V/J alleles. A κ-producing cell that displayed the unusual finding of λ gene rearrangement demonstrated a rearranged Kde. This rearrangement was a …
Mammalian Genome Organization: An Evolutionary View, S. J. O'Brien, H. N.J.E. Seuanez Womack
Mammalian Genome Organization: An Evolutionary View, S. J. O'Brien, H. N.J.E. Seuanez Womack
Biology Faculty Articles
No abstract provided.
Isolation Of A Cdna Clone For The Human Laminin-B1 Chain And Its Gene Localization, Michael Jaye, William Modi, George A. Ricca, Robert Mudd, Inc-Ming Chiu, Stephen J. O'Brien, William N. Drohan
Isolation Of A Cdna Clone For The Human Laminin-B1 Chain And Its Gene Localization, Michael Jaye, William Modi, George A. Ricca, Robert Mudd, Inc-Ming Chiu, Stephen J. O'Brien, William N. Drohan
Biology Faculty Articles
A cDNA clone encoding the B1 chain of human laminin has been isolated from a human endothelial cell cDNA library. With use of this probe and a panel of rodent/human somatic-cell hybrids and in situ hybridization, the gene for the human laminin-B1 chain has been localized to chromosome 7, band q31.
A Novel Form Of Human Polymorphism Involving The Hdhfr-Ψ1 Pseudogene Identifies Three Rflps, Nicholas P. Anagnou, S. E. Antonarakis, Stephen J. O'Brien, Arthur W. Nienhuis
A Novel Form Of Human Polymorphism Involving The Hdhfr-Ψ1 Pseudogene Identifies Three Rflps, Nicholas P. Anagnou, S. E. Antonarakis, Stephen J. O'Brien, Arthur W. Nienhuis
Biology Faculty Articles
No abstract provided.
A New Moderately Repetitive Dna Sequence Family Of Novel Organization, Neal D. Epstein, S. Karlsson, Stephen J. O'Brien, William S. Modi, A. Moulton, Arthur W. Nienhuis
A New Moderately Repetitive Dna Sequence Family Of Novel Organization, Neal D. Epstein, S. Karlsson, Stephen J. O'Brien, William S. Modi, A. Moulton, Arthur W. Nienhuis
Biology Faculty Articles
In cloning adenovirus homologous sequences, from a human cosmid library, we identified a moderately repetitive DNA sequence family consisting of tandem arrays of 2.5 kb members. A member was sequenced and several non-adjacent, 15–20 bp G-C rich segments with homology to the left side of adenovirus were discovered. The copy number of 400 members is highly conserved among humans. Southern blots of partial digests of human DNA have verified the tandem array of the sequence family. The chromosomal location was defined by somatic cell genetics and in situ hybridization. Tandem arrays are found only on chromosomes 4 (4q31) and 19 …
Genetic Characterization Of Human C-Rel Sequences, Elise Brownell, Stephen J. O'Brien, William Nash, Nancy Rice
Genetic Characterization Of Human C-Rel Sequences, Elise Brownell, Stephen J. O'Brien, William Nash, Nancy Rice
Biology Faculty Articles
We isolated and sequenced a human genomic-DNA segment that is homologous to a portion of v-rel, the transforming gene of reticuloendotheliosis virus (strain T). We also localized the human rel sequences to human chromosome 2 by screening a panel of rodent x human somatic-cell hybrids with the newly described human rel segment.
Amplification And Chromosomal Dispersion Of Human Endogenous Retroviral Sequences, Paul E. Steele, Malcolm A. Martin, Arnold B. Rabson, Theodore Bryan, Stephen J. O'Brien
Amplification And Chromosomal Dispersion Of Human Endogenous Retroviral Sequences, Paul E. Steele, Malcolm A. Martin, Arnold B. Rabson, Theodore Bryan, Stephen J. O'Brien
Biology Faculty Articles
Endogenous retroviral sequences in humans have undergone amplification events involving both viral and flanking cellular sequences. We cloned members of an amplified family of full-length endogenous retroviral sequences. Genomic blotting, employing a flanking cellular DNA probe derived from a member of this family, revealed a similar array of reactive bands in both humans and chimpanzees, indicating that an amplification event involving retroviral and associated cellular DNA sequences occurred before the evolutionary separation of these two primates. Southern analyses of restricted somatic cell hybrid DNA preparations suggested that endogenous retroviral segments are widely dispersed in the human genome and that amplification …
Conserved Chromosomal Positions Of Dual Domains Of The Ets Protooncogene In Cats, Mice, And Humans, Dennis K. Watson, Mary J. Mcwilliams-Smith, Christine Kozak, Roger Reeves, John Gearheart, Michael F. Nunn, William Nash, John R. Fowle Iii, Peter Duesberg, Takis S. Papas, Stephen J. O'Brien
Conserved Chromosomal Positions Of Dual Domains Of The Ets Protooncogene In Cats, Mice, And Humans, Dennis K. Watson, Mary J. Mcwilliams-Smith, Christine Kozak, Roger Reeves, John Gearheart, Michael F. Nunn, William Nash, John R. Fowle Iii, Peter Duesberg, Takis S. Papas, Stephen J. O'Brien
Biology Faculty Articles
The mammalian protooncogene homologue of the avian v-ets sequence from the E26 retrovirus consists of two sequentially distinct domains located on different chromosomes. Using somatic cell hybrid panels, we have mapped the mammalian homologue of the 5' v-ets-domain to chromosome 11 (ETS1) in man, to chromosome 9 (Ets-1) in mouse, and to chromosome D1 (ETS1) in the domestic cat. The mammalian homologue of the 3' v-ets domain was similarly mapped to human chromosome 21 (ETS2), to mouse chromosome 16 (Ets-2), and to feline chromosome C2 (ETS2). …
The Ets Sequence From The Transforming Gene Of Avian Erythroblastosis Virus, E26, Has Unique Domains On Human Chromosomes 11 And 21: Both Loci Are Transcriptionally Active, Dennis K. Watson, Mary J. Mcwilliams-Smith, M. F. Nunn, Peter Duesberg, Stephen J. O'Brien, Takis S. Papas
The Ets Sequence From The Transforming Gene Of Avian Erythroblastosis Virus, E26, Has Unique Domains On Human Chromosomes 11 And 21: Both Loci Are Transcriptionally Active, Dennis K. Watson, Mary J. Mcwilliams-Smith, M. F. Nunn, Peter Duesberg, Stephen J. O'Brien, Takis S. Papas
Biology Faculty Articles
Human DNA segments homologous to the ets region from the transforming gene of avian erythroblastosis virus, E26, were molecularly cloned and shown to be closely related to the viral equivalent by hybridization and partial sequence analysis. The transforming gene of E26 has a tripartite origin with the structure ∆gag [1.2 kilobases (kb) from the viral gag gene]-myb(0.9 kb from the chicken myb gene)-ets (1.6 kb from the chicken ets gene). Human ets DNA is located on two distinct human chromosomes. The human ets-1 locus on chromosome 11 encodes a single mRNA of 6.8 kb; the second locus, …
Genetic Mapping Of Endogenous Rd-114 Retroviral Sequences Of Domestic Cats, Roger H. Reeves, William G. Nash, Stephen J. O'Brien
Genetic Mapping Of Endogenous Rd-114 Retroviral Sequences Of Domestic Cats, Roger H. Reeves, William G. Nash, Stephen J. O'Brien
Biology Faculty Articles
The RD-114 family of endogenous retroviral sequences in domestic cats has been shown to consist of approximately 20 copies of genetically divergent virogenes per haploid genome. The chromosomal localization for four endogenous sequences (RDV1-4) was accomplished by correlating the occurrence of specific feline chromosomes with diagnostic viral DNA fragments in a panel of cat x rodent somatic cell hybrids. Analysis of the hybrid panel revealed that endogenous RD-114 sequences are dispersed on multiple cat chromosomes, that certain proviral segments are polymorphic with respect to the presence or absence of virus, and that a restriction fragment characteristic of inducible RD-114 resides …
Twenty-Seven Protein Polymorphisms By Two-Dimensional Electrophoresis Of Serum, Erythrocytes, And Fibroblasts In Two Pedigrees, David Goldman, Lynn R. Goldin, P. Rathnagiri, Stephen J. O'Brien, Janice A. Egeland, Carl R. Merril
Twenty-Seven Protein Polymorphisms By Two-Dimensional Electrophoresis Of Serum, Erythrocytes, And Fibroblasts In Two Pedigrees, David Goldman, Lynn R. Goldin, P. Rathnagiri, Stephen J. O'Brien, Janice A. Egeland, Carl R. Merril
Biology Faculty Articles
Twenty-seven independent polymorphic loci were detected by two-dimensional electrophoresis (2DE) of serum, erythrocytes, and fibroblasts in two large families and analyzed for linkage to classical genetic markers. We detected seven serum, four erythrocyte, and 17 fibroblast protein loci that exhibited charge variation in these two families and in a sample of unrelated individuals. The genetic basis of protein variants was confirmed by quantitative gene-dosage dependence and by conformance to Mendelian transmission in the two families, except for four rare variants for which transmission analysis was not possible. Linkage analysis demonstrated that each of the variants represent products of independent loci, …
Molecular Genetic Characterization Of The Rd-114 Gene Family Of Endogenous Feline Retroviral Sequences, Roger H. Reeves, Stephen J. O'Brien
Molecular Genetic Characterization Of The Rd-114 Gene Family Of Endogenous Feline Retroviral Sequences, Roger H. Reeves, Stephen J. O'Brien
Biology Faculty Articles
RD-114 is a replication-competent, xenotropic retrovirus which is homologous to a family of moderately repetitive DNA sequences present at ca. 20 copies in the normal cellular genome of domestic cats. To examine the extent and character of genomic divergence of the RD-114 gene family as well as to assess their positional association within the cat genome, we have prepared a series of molecular clones of endogenous RD-114 DNA segments from a genomic library of cat cellular DNA. Their restriction endonuclease maps were compared with each other as well as to that of the prototype-inducible RD-114 which was molecularly cloned from …
On The Distribution And Characteristics Of Isozyme Expression In Mycoplasma, Acholeplasma, And Ureaplasma Species, Stephen J. O'Brien, Janice M. Simonson, S. Razin, Michael F. Barile
On The Distribution And Characteristics Of Isozyme Expression In Mycoplasma, Acholeplasma, And Ureaplasma Species, Stephen J. O'Brien, Janice M. Simonson, S. Razin, Michael F. Barile
Biology Faculty Articles
A summary of a survey of three genera of mycoplasmatales (Mycoplasma, Acholeplasma, and Ureaplasma) for isozyme expression is presented. Isozyme analysis of mycoplasmas has been employed in at least three distinct areas: (1) as genetic markers for identification, individualization, and taxonomic classification; (2) as markers for cell culture contamination; and (3) as a qualitative measure of the operative metabolic pathways in the diverse species. We have found five ubiquitous enzymes: purine nucleoside phosphorylase, adenylate kinase, inorganic pyrophosphatase, dipeptidase, and esterase. Three enzymes, glucose-6-phosphate dehydrogenase, phosphogluconate dehydrogenase, and superoxide dismutase, were restricted to Acholeplasma species and were not …
Dispermic Origin And Clinical Outcome Of Three Complete Hydatidiform Moles With 46,Xy Karyotype, U. Surti, A. E. Szulman, S. O'Brien
Dispermic Origin And Clinical Outcome Of Three Complete Hydatidiform Moles With 46,Xy Karyotype, U. Surti, A. E. Szulman, S. O'Brien
Biology Faculty Articles
Three new cases of complete, classic hydatidiform mole with a 46,XY karyotype are described. They originated by dispermy as demonstrated by chromosome and enzyme analyses. Levels of human chorionic gonadotropin decreased to normal spontaneously within a short time, indicating a benign course in these three cases. Of a total of 18 cases reported to date, postoperative clinical information was available for 10 patients. Two of these 10 patients had a malignant course with lung metastases. It is of theoretical and clinical importance to establish the magnitude of the risk of malignancy for hydatidiform mole with a 46,XY karyotype. © 1982.
Evidence For The Horizontal Acquisition Of Murine Akr Virogenes By Recent Horizontal Infection Of The Germ Line, Stephen J. O'Brien, Janet L. Moore, Malcolm A. Martin, James E. Womack
Evidence For The Horizontal Acquisition Of Murine Akr Virogenes By Recent Horizontal Infection Of The Germ Line, Stephen J. O'Brien, Janet L. Moore, Malcolm A. Martin, James E. Womack
Biology Faculty Articles
Several recent reports have established the biological and molecular genetic similarity between the endogenous AKV virus of strain AKR, and an N-ecotropic endogenous virus found in the genome of feral Japanese mice, Mus musculus molossinus. The similarities are so striking as to suggest a common origin of these viruses, which are present in some, but not all, inbred mouse strains. The virogenes of AKR mice may have been acquired by either: (a) common descent of AKR (and other AKV+ strains) from a common ancestor of AKR and molossinus animals, or (b) horizontal germ line infection of the AKR …