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Genetics and Genomics Commons™

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University of Nebraska - Lincoln

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Articles 91 - 120 of 1202

Full-Text Articles in Genetics and Genomics

Mineral Composition Of Serially Slaughtered Holstein Steers Supplemented With Zilpaterol Hydrochloride, Andrea K. Watson, Trent J. Mcevers, Lee-Anne J. Walter, Nathan D. May, Jacob A. Reed, J. C. Macdonald, Galen E. Erickson, N. Andy Cole, Johnjohn P. Hutcheson, Ty E. Lawrence Jun 2023

Mineral Composition Of Serially Slaughtered Holstein Steers Supplemented With Zilpaterol Hydrochloride, Andrea K. Watson, Trent J. Mcevers, Lee-Anne J. Walter, Nathan D. May, Jacob A. Reed, J. C. Macdonald, Galen E. Erickson, N. Andy Cole, Johnjohn P. Hutcheson, Ty E. Lawrence

Department of Animal Science: Faculty Publications

Calf-fed Holstein steers (n = 115; 449 ± 20 kg) were utilized in a serial harvest experiment. A baseline group of five steers was harvested after 226 d on feed (DOF), which was designated day 0. The remaining cattle were assigned randomly to 11 harvest groups, with slaughter every 28 d. Cattle were either not (CON) or were fed zilpaterol hydrochloride for 20 d followed by a 3 d withdrawal (ZH). There were five steers per treatment in each slaughter group ranging from days 28 to 308. Whole carcasses were divided into lean, …

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Viral Dna Accumulation Regulates Replication Efficiency Of Chlorovirus Osy-Ne5 In Two Closely Related Chlorella Variabilis Strains, Ahmed Esmael, Irina V. Agarkova, David D. Dunigan, You Zhou, James L. Van Etten Jun 2023

Viral Dna Accumulation Regulates Replication Efficiency Of Chlorovirus Osy-Ne5 In Two Closely Related Chlorella Variabilis Strains, Ahmed Esmael, Irina V. Agarkova, David D. Dunigan, You Zhou, James L. Van Etten

Nebraska Center for Virology: Faculty Publications

Many chloroviruses replicate in Chlorella variabilis algal strains that are ex-endosymbionts isolated from the protozoan Paramecium bursaria, including the NC64A and Syngen 2-3 strains. We noticed that indigenous water samples produced a higher number of plaque-forming viruses on C. variabilis Syngen 2-3 lawns than on C. variabilis NC64A lawns. These observed differences led to the discovery of viruses that replicate exclusively in Syngen 2-3 cells, named Only Syngen (OSy) viruses. Here, we demonstrate that OSy viruses initiate infection in the restricted host NC64A by synthesizing some early virus gene products and that approximately 20% of the cells produce a …

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Basic Fibroblast Growth Factor Induces Proliferation And Collagen Production By Fibroblasts Derived From The Bovine Corpus Luteum, Corrine F. Monaco, Michele R. Plewes, Emilia Przygrodzka, Jitu W. George, Fang Qiu, Peng Xiao, Jennifer R. Wood, Andrea S. Cupp, John S. Davis Jun 2023

Basic Fibroblast Growth Factor Induces Proliferation And Collagen Production By Fibroblasts Derived From The Bovine Corpus Luteum, Corrine F. Monaco, Michele R. Plewes, Emilia Przygrodzka, Jitu W. George, Fang Qiu, Peng Xiao, Jennifer R. Wood, Andrea S. Cupp, John S. Davis

Department of Animal Science: Faculty Publications

Cyclic regression of the ovarian corpus luteum, the endocrine gland responsible for progesterone production, involves rapid matrix remodeling. Despite fibroblasts in other systems being known for producing and maintaining extracellular matrix, little is known about fibroblasts in the functional or regressing corpus luteum. Vast transcriptomic changes occur in the regressing corpus luteum, among which are reduced levels of vascular endothelial growth factor A (VEGFA) and increased expression of fibroblast growth factor 2 (FGF2) after 4 and 12 h of induced regression, when progesterone is declining and the microvasculature is destabilizing. We hypothesized that FGF2 activates luteal …

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Impact Of Cattle Grazing Corn Residue In Spring On Soil And Subsequent Soybean Yield, Morgan T. Grabau, J. C. Macdonald, Zach E. Carlson, M. E. Drewnoski May 2023

Impact Of Cattle Grazing Corn Residue In Spring On Soil And Subsequent Soybean Yield, Morgan T. Grabau, J. C. Macdonald, Zach E. Carlson, M. E. Drewnoski

Department of Animal Science: Faculty Publications

The effects of spring corn (Zea mays) residue grazing and stocking density on soil physical properties and subsequent soybean (Glycine max) yield were evaluated in an experiment with three treatments: no grazing (NG), normal stocking density (NSD), and high stocking density (HSD). The study was conducted over 2 years with four replicates per treatment each year. Steers (277 ± 4.4 kg) were stocked at 7.5 steers/ha for NSD with a target grazing of 45 days starting in mid- February, and 22.5 steers/ha for HSD with a target grazing of 15 days in mid-March, such that the …

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Impact Of Shade In Beef Feedyards On Performance, Ear Temperature, And Heat Stress Measures, Thomas M. Winders, Brett A. Melton, Boyd M. Boyd, Casey N. Macken, J. C. Macdonald, Galen E. Erickson May 2023

Impact Of Shade In Beef Feedyards On Performance, Ear Temperature, And Heat Stress Measures, Thomas M. Winders, Brett A. Melton, Boyd M. Boyd, Casey N. Macken, J. C. Macdonald, Galen E. Erickson

Department of Animal Science: Faculty Publications

A 2-yr study (year 1: March to September 2017; year 2: February to August 2018) was conducted using crossbred steers (year 1: n = 1677; initial body weight [BW] = 372 kg, SD = 47; year 2: n = 1713; initial BW = 379 kg, SD = 10) in a commercial feedyard study in Eastern NE to determine the effects of shade on cattle performance, ear temperature, and cattle activity. Two treatments were evaluated using a randomized complete block design (n = 5 blocks based on arrival). Treatments were assigned randomly to pens and consisted of five pens without …

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Influenza C And D Viruses Demonstrated A Differential Respiratory Tissue Tropism In A Comparative Pathogenesis Study In Guinea Pigs, Chithra C. Sreenivasan, Runxia Liu, Rongyuan Gao, Yicheng Guo, Ben M. Hause, Milton Thomas, Ahsan Naveed, Travis Clement, Dana Rausch, Jane Christopher-Hennings, Eric Nelson, Julian Druce, Miaoyun Zhao, Radhey S. Kaushik, Qingsheng Li, Zizhang Sheng, Dan Dan, Feng Li May 2023

Influenza C And D Viruses Demonstrated A Differential Respiratory Tissue Tropism In A Comparative Pathogenesis Study In Guinea Pigs, Chithra C. Sreenivasan, Runxia Liu, Rongyuan Gao, Yicheng Guo, Ben M. Hause, Milton Thomas, Ahsan Naveed, Travis Clement, Dana Rausch, Jane Christopher-Hennings, Eric Nelson, Julian Druce, Miaoyun Zhao, Radhey S. Kaushik, Qingsheng Li, Zizhang Sheng, Dan Dan, Feng Li

Nebraska Center for Virology: Faculty Publications

Influenza C virus (ICV) is increasingly associated with community-acquired pneumonia (CAP) in children and its disease severity is worse than the influenza B virus, but similar to influenza A virus associated CAP. Despite the ubiquitous infection landscape of ICV in humans, little is known about its replication and pathobiology in animals. The goal of this study was to understand the replication kinetics, tissue tropism, and pathogenesis of human ICV (huICV) in comparison to the swine influenza D virus (swIDV) in guinea pigs. Intranasal inoculation of both viruses did not cause clinical signs, however, the infected animals shed virus in nasal …

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Adenoviral-Vectored Epigraph Vaccine Elicits Robust, Durable, And Protective Immunity Against H3 Influenza A Virus In Swine, Erika M. Petro-Turnquist, Matthew J. Pekarek, Nicholas Jeanjaquet, Cedric Wooledge, David J. Steffen, Hiep Vu, Eric A. Weaver May 2023

Adenoviral-Vectored Epigraph Vaccine Elicits Robust, Durable, And Protective Immunity Against H3 Influenza A Virus In Swine, Erika M. Petro-Turnquist, Matthew J. Pekarek, Nicholas Jeanjaquet, Cedric Wooledge, David J. Steffen, Hiep Vu, Eric A. Weaver

Nebraska Center for Virology: Faculty Publications

Current methods of vaccination against swine Influenza A Virus (IAV-S) in pigs are infrequently updated, induce strain-specific responses, and have a limited duration of protection. Here, we characterize the onset and duration of adaptive immune responses after vaccination with an adenoviral-vectored Epigraph vaccine. In this longitudinal study we observed robust and durable antibody responses that remained above protective titers six months after vaccination. We further identified stable levels of antigen-specific T cell responses that remained detectable in the absence of antigen stimulation. Antibody isotyping revealed robust class switching from IgM to IgG induced by Epigraph vaccination, while the commercial comparator …

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Prostaglandin F2Α Regulates Mitochondrial Dynamics And Mitophagy In The Bovine Corpus Luteum, Michele R. Plewes, Emilia Przygrodzka, Corrine F. Monaco, Alexandria P. Snider, Jessica Jessica Keane, Patrick D. Burns, Jennifer R. Wood, Andrea S. Cupp, John S. Davis May 2023

Prostaglandin F2Α Regulates Mitochondrial Dynamics And Mitophagy In The Bovine Corpus Luteum, Michele R. Plewes, Emilia Przygrodzka, Corrine F. Monaco, Alexandria P. Snider, Jessica Jessica Keane, Patrick D. Burns, Jennifer R. Wood, Andrea S. Cupp, John S. Davis

Department of Animal Science: Faculty Publications

Prostaglandins are arachidonic acid-derived lipid mediators involved in numerous physiological and pathological processes. PGF2α analogues are therapeutically used for regulating mammalian reproductive cycles and blood pressure, inducing term labor, and treating ocular disorders. PGF2α exerts effects via activation of calcium and PKC signaling, however, little is known about the cellular events imposed by PGF2α signaling. Here, we explored the early effects of PGF2α on mitochondrial dynamics and mitophagy in the bovine corpus luteum employing relevant and well characterized in vivo and in vitro approaches. We identified PKC/ERK and AMPK as critical protein kinases essential for activation of mitochondrial fission proteins, …

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Utilizing NuTrack To Access The Activity Levels In Pigs With Varying Degrees Of Genetic Potential For Growth And Feed Intake, Dalton Obermier, Melanie Trenahile-Grannemann, Ty B. Schmidt, Tom Rathje, Benny Mote May 2023

Utilizing NuTrack To Access The Activity Levels In Pigs With Varying Degrees Of Genetic Potential For Growth And Feed Intake, Dalton Obermier, Melanie Trenahile-Grannemann, Ty B. Schmidt, Tom Rathje, Benny Mote

Department of Animal Science: Faculty Publications

Feed cost accounts for over two-thirds of the variable cost of production. In order to reduce feed costs without sacrificing production numbers, feed efficiency must be improved. Calorie expenditure has been difficult to quantify in the past but is understood to impact residual feed intake (RFI) greatly. The objective of this work was to utilize an advanced computer vision system to evaluate activity levels across sex and sire groups with different expected breeding value combinations for growth and feed intake. A total of 199 pigs from four different sire groups (DNA Genetics Line 600) High Feed Intake/High Growth (HIHG), Low …

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Appearance Of Choline Metabolites In Plasma And Milk When Choline Is Infused Into The Abomasum With Or Without Methionine, C. J.R. Jenkins, Paul J. Kononoff May 2023

Appearance Of Choline Metabolites In Plasma And Milk When Choline Is Infused Into The Abomasum With Or Without Methionine, C. J.R. Jenkins, Paul J. Kononoff

Department of Animal Science: Faculty Publications

Four lactating, ruminally cannulated Jersey cows, (mean ± standard deviation) 264 ± 54.2 d in milk and 484 ± 24.1 kg of body weight, were arranged in a 4 × 4 Latin square design to measure the effects of abomasal infusion of choline chloride with or without dl-Met on milk and plasma choline metabolites and plasma AA in cows fed a Met-deficient diet. Cows were randomly assigned to 1 of 4 experimental treatments: (1) control; no supplemental Met or choline (CON), (2) 13 g/d of choline ion delivered via abomasal infusion (CHO), (3) 13 g/d of Met delivered via abomasal …

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Gene Expression Changes Linked To Phenylpropanoid-Based Resistance To Fusarium Head Blight Of Wheat, Shiv Singla May 2023

Gene Expression Changes Linked To Phenylpropanoid-Based Resistance To Fusarium Head Blight Of Wheat, Shiv Singla

Department of Plant Pathology: Dissertations, Theses, and Student Research

Fusarium graminearum is a devastating pathogen of wheat that causes Fusarium head blight (FHB) and contaminates the grain with the mycotoxin deoxynivalenol (DON). Resistance to FHB is quantitative and it is important to identify additional genes conferring resistance against it. The goal of this thesis was to examine if the constitutive expression of two sorghum phenylpropanoid pathway genes, SbCCoAOMT (encoding caffeoyl-CoA O-methyltransferase) and SbC3’H (encoding p-coumarate 3-hydroxylase), in the moderately-susceptible spring wheat CB037 can provide Type-I and Type-II resistance to F. graminearum and determine the underlying mechanisms of the enhanced resistance. The constitutive expression lines (CCoAOMT413, CCoAOMT421, C3H112, …

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A Review Of How Bioinformatics And Genome Sequencing Are Affecting Precision Medicine, Taylor S. Hickey May 2023

A Review Of How Bioinformatics And Genome Sequencing Are Affecting Precision Medicine, Taylor S. Hickey

Honors Program: Senior Projects (Public)

Advancement in genomic sequencing and bioinformatics methods have been affecting biomedical research through precision medicine, especially in the area of cancer. Vaccine therapies can be developed using neoantigens that target specific mutations in tumors. The goals of this research are to identify mutations that lead to cancer and then define subpopulations in which patients can easily be identified. The future goal is to have targeted vaccines that are specific to each subpopulation ready to be used in treatment of their cancer. Limitations to reaching these goals have been due to tumor heterogeneity, cancer location, and difficulty in creating neoantigens for …

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Urbanization Affects Web Abundance And Aggregation Of A Funnel‑Weaving Spider, Agelenopsis Pennsylvanica (Agelenidae), Brandi J. Pessman, Madison Hays, Earl Agpawa, Eileen Hebets May 2023

Urbanization Affects Web Abundance And Aggregation Of A Funnel‑Weaving Spider, Agelenopsis Pennsylvanica (Agelenidae), Brandi J. Pessman, Madison Hays, Earl Agpawa, Eileen Hebets

Eileen Hebets Publications

Animals distribute themselves within habitats based on a variety of environmental conditions, including those impacted by urbanization. Suspected global declines in urban arthropod biodiversity have required that we examine how urban conditions affect the distribution of this ecologically important group. Throughout North America, funnel-weaving spiders (Agelenidae) are prevalent across urban habitats and actively choose sites to build webs. We compared Agelenopsis pennsylvanica abundance and distribution between two distinct urban habitats: an urban center (university campus) and an urban forest (city park). These urban habitats differed significantly in features like plant diversity and proximity to roads and highways. We searched along …

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Dataset Of Rna-Seq Transcriptome Of The Fetal Liver At Day 83 Of Gestation Associated With Periconceptual Maternal Nutrition In Beef Heifers, Wellison J.S. Diniz, Alison K. Ward, Kacie L. Mccathy, Cierrah J. Kassetas, Friederike Baumgaertner, Lawrence P. Reynolds, Pawel P. Borowicz, Kevin K. Sedivec, James D. Kirsch, Sheri T. Dorsam, Tammi L. Neville, J. Chris Forcherio, Ronald Scott, Joel S. Caton, Carl R. Dahlen Apr 2023

Dataset Of Rna-Seq Transcriptome Of The Fetal Liver At Day 83 Of Gestation Associated With Periconceptual Maternal Nutrition In Beef Heifers, Wellison J.S. Diniz, Alison K. Ward, Kacie L. Mccathy, Cierrah J. Kassetas, Friederike Baumgaertner, Lawrence P. Reynolds, Pawel P. Borowicz, Kevin K. Sedivec, James D. Kirsch, Sheri T. Dorsam, Tammi L. Neville, J. Chris Forcherio, Ronald Scott, Joel S. Caton, Carl R. Dahlen

Department of Animal Science: Faculty Publications

Herein, we present a dataset based on the RNA-Seq analysis of liver tissue from bovine female fetuses at day 83 of gestation. The findings were reported in the main article, “Periconceptual maternal nutrition affects fetal liver programming of energy- and lipid-related genes”[1] . These data were generated to investigate the effects of periconceptual maternal vitamin and mineral supplementation and rates of body weight gain on the transcript abundance of genes associated with fetal hepatic metabolism and function. To this end, crossbred Angus beef heifers ( n = 35) were randomly assigned to 1 of 4 treatments in a 2 × …

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Influence Of Ambient Water Coloration On Habitat And Conspecific Choice In The Female Lake Malawi Cichlid, Metriaclima Zebra, Noori Choi, Nicolas Mathevon, Eileen A. Hebets, Marilyn Beauchaud Apr 2023

Influence Of Ambient Water Coloration On Habitat And Conspecific Choice In The Female Lake Malawi Cichlid, Metriaclima Zebra, Noori Choi, Nicolas Mathevon, Eileen A. Hebets, Marilyn Beauchaud

Eileen Hebets Publications

Female cichlid fish living in African great lakes are known to have sensory systems that are adapted to ambient light environments. These sensory system adaptations are hypothesized to have influenced the evolution of the diverse male nuptial coloration. In rock-dwelling Lake Malawi mbuna cichlids, however, the extent to which ambient light environments influence female sensory systems and potentially associated male nuptial coloration remains unknown. Yet, the ubiquitous blue flank coloration and UV reflection of male mbuna cichlids suggest the potential impacts of the blue-shifted ambient light environment on these cichlid’s visual perception and male nuptial coloration in the shallow water …

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Dataset Of Rna-Seq Transcriptome Of The Fetal Liver At Day 83 Of Gestation Associated With Periconceptual Maternal Nutrition In Beef Heifers, Wellison J.S. Diniz, Alison K. Ward, Kacie L. Mccathy, Cierrah J. Kassetas, Friederike Baumgaertner, Lawrence P. Reynolds, Pawel P. Borowicz, Kevin K. Sedivec, James D. Kirsch, Sheri T. Dorsam, Tammi L. Neville, J. Chris Forcherio, Ronald Scott, Joel S. Caton, Carl R. Dahlen Apr 2023

Dataset Of Rna-Seq Transcriptome Of The Fetal Liver At Day 83 Of Gestation Associated With Periconceptual Maternal Nutrition In Beef Heifers, Wellison J.S. Diniz, Alison K. Ward, Kacie L. Mccathy, Cierrah J. Kassetas, Friederike Baumgaertner, Lawrence P. Reynolds, Pawel P. Borowicz, Kevin K. Sedivec, James D. Kirsch, Sheri T. Dorsam, Tammi L. Neville, J. Chris Forcherio, Ronald Scott, Joel S. Caton, Carl R. Dahlen

Department of Animal Science: Faculty Publications

Herein, we present a dataset based on the RNA-Seq analysis of liver tissue from bovine female fetuses at day 83 of gestation. The findings were reported in the main article, “Periconceptual maternal nutrition affects fetal liver programming of energy- and lipid-related genes”[1] . These data were generated to investigate the effects of periconceptual maternal vitamin and mineral supplementation and rates of body weight gain on the transcript abundance of genes associated with fetal hepatic metabolism and function. To this end, crossbred Angus beef heifers ( n = 35) were randomly assigned to 1 of 4 treatments in a 2 ×2 …

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Application Of A Novel Lytic Jerseyvirus Phage Lpsent1 For The Biological Control Of The Multidrug-Resistant Salmonella Enteritidis In Foods, Rashad R. Al-Hindi, Mona G. Alharbi, Ibrahim Alotibi, Sheren A. Azhari, Khloud M. Algothmi, Ahmed Esmael Apr 2023

Application Of A Novel Lytic Jerseyvirus Phage Lpsent1 For The Biological Control Of The Multidrug-Resistant Salmonella Enteritidis In Foods, Rashad R. Al-Hindi, Mona G. Alharbi, Ibrahim Alotibi, Sheren A. Azhari, Khloud M. Algothmi, Ahmed Esmael

Nebraska Center for Virology: Faculty Publications

Non-typhoidal Salmonella is the tremendously predominant source of acquired foodborne infection in humans, causing salmonellosis which is a global threat to the healthcare system. This threat is even worse when it is combined with the incidence of multidrug-resistant Salmonella strains. Bacteriophage therapy has been proposed as a promising potential candidate to control a diversity of foodborne infective bacteria. The objective of this study designed to isolate and characterize lytic phages infecting zoonotic multi-drug resistant and strong biofilm producer Salmonella enterica serovar Enteritidis EG.SmE1 and then apply the isolated phage/s as a biocontrol agent against infections in ready-to-eat food articles including …

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The Sars-Cov-2 Utr’S Intrudes Host Rbp’S And Modulates Cellular Splicing, Anjali Singh, Kush Kumar Pandey, Shubham Kumar Agrawal, Rupesh K. Srivastava, Sankar Bhattacharyya, Bhupendra Verma Apr 2023

The Sars-Cov-2 Utr’S Intrudes Host Rbp’S And Modulates Cellular Splicing, Anjali Singh, Kush Kumar Pandey, Shubham Kumar Agrawal, Rupesh K. Srivastava, Sankar Bhattacharyya, Bhupendra Verma

Nebraska Center for Virology: Faculty Publications

SARS-CoV-2 is a novel coronavirus that causes a potentially fatal respiratory disease known as coronavirus disease (COVID-19) and is responsible for the ongoing pandemic with increasing mortality. Understanding the host-virus interaction involved in SARS-CoV-2 pathophysiology will enhance our understanding of the mechanistic basis of COVID-19 infection. The characterization of post-transcriptional gene regulatory networks, particularly pre-mRNA splicing, and the identification and characterization of host proteins interacting with the 5' and 3'UTRs of SARS-CoV-2 will improve our understanding of posttranscriptional gene regulation during SARS-CoV-2 pathogenesis. Here, we demonstrate that either SARS-CoV-2 infection or exogenous overexpression of the 5' and 3’UTRs of the …

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Evaluating Draft Epa Emissions Models For Laying Hen Facilities, Yijie Xiong, Guoming Li, Brett C. Ramirez, Robert T. Burns, Richard S. Gates Apr 2023

Evaluating Draft Epa Emissions Models For Laying Hen Facilities, Yijie Xiong, Guoming Li, Brett C. Ramirez, Robert T. Burns, Richard S. Gates

Department of Animal Science: Faculty Publications

In August 2021, the U.S. Environmental Protection Agency (EPA) released draft models to estimate daily NH3, H2S, PM10, PM2.5, and TSP emissions from egg-layer houses (high-rise and manure-belt) and manure storage using inputs of daily mean ambient temperature, relative humidity (RH), and hen inventory. These models were developed from refined datasets generated by the National Air Emissions Monitoring Study fieldwork completed in 2009. Notably, they do not include data for cage-free housing. Currently, 66% of U.S. laying hens are housed in cages; thus, these models, if adopted, will have a substantial impact …

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White Coat Color Of A Black Angus Calf Attributed To An Occurrence Of The Delr217 Variant Of Mitf, Jessica Lynn Petersen, Renae L. Sieck, David J. Steffen Apr 2023

White Coat Color Of A Black Angus Calf Attributed To An Occurrence Of The Delr217 Variant Of Mitf, Jessica Lynn Petersen, Renae L. Sieck, David J. Steffen

Department of Animal Science: Faculty Publications

A white calf, with minimal pigmented markings, was born to two registered Black Angus parents. Given the possibility of an unknown recessive or de novo dominant mutation, whole-genome sequencing was conducted on the trio of individuals. A 3-bp in-frame deletion in MITF was identified; this mutation was unique to the calf but identical to the delR217 variant reported in both humans and murine models of Waardenburg syndrome type 2A and Tietz syndrome. Given the coat color phenotype and identity of the mutation, our data support that this calf represents the first instance of this recurring MITF mutation in cattle.

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The Effects Of Temperature And Mito-Nuclear Interaction On Male Fertility In Drosophila., Violetta Bakunina Mar 2023

The Effects Of Temperature And Mito-Nuclear Interaction On Male Fertility In Drosophila., Violetta Bakunina

Honors Program: Senior Projects (Public)

The (simw501);Ore genotype is a strain of Drosophila melanogaster that has impaired mitochondrial function as a result of a disrupted interaction between a mitochondrially-encoded tRNATyr and the nuclear-encoded synthetase that aminoacylates mt-tRNATyr. We hypothesized that disruption of mitochondrial protein synthesis and oxidative phosphorylation (OXPHOS) in this genotype decreases mitochondrial translation and results in the sterility of (simw501);OreR males at 28oC. The results of Mitotracker staining showcased the reduced Mean Fluorescence Intensity (MFI), which confirms that sterility has an energetic basis, yet also that mature sperm function is compromised. Cytological dissection of male gonads …

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Pen Surface Temperature As A Predictor Of Dailywater Intake And Tympanic Temperature In Steers Finished In Feedlots, Rodrigo A. Arias, Terry L. Mader Mar 2023

Pen Surface Temperature As A Predictor Of Dailywater Intake And Tympanic Temperature In Steers Finished In Feedlots, Rodrigo A. Arias, Terry L. Mader

Department of Animal Science: Faculty Publications

Adequate estimation of water demand in cattle production feed yards is of high importance, especially due to reduced water availability because of changes in rain precipitation patterns and amounts. The pen surface in feed yards receives and reflects solar radiation, affecting along with other factors the microclimate to which cattle are exposed. This study aimed to describe the relationship between the pen surface temperature with the daily water intake and the tympanic temperature of finishing steers. Climate variables, including solar radiation, air temperature, relative humidity, and wind speed plus pen surface temperature and soil temperature at 10.2 cm depth were …

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Evaluation Of Four Thermal Comfort Indices And Their Relationship With Physiological Variables In Feedlot Cattle, Rodrigo A. Arias, Terry L. Mader Mar 2023

Evaluation Of Four Thermal Comfort Indices And Their Relationship With Physiological Variables In Feedlot Cattle, Rodrigo A. Arias, Terry L. Mader

Department of Animal Science: Faculty Publications

Climatic data from different years and experiments conducted in Nebraska were used to estimate four comfort thermal indices and to predict the risk of heat stress and its relationship with pen surface temperature (PST). These included the temperature–humidity index (THI), the adjusted THI (THIadj), the heat load index (HLI), and THIPST using pen surface temperature instead of air temperature. Respiration rates (RR), tympanic temperatures (TT), and panting scores (PS) were also collected in each year and from each location. During 2007, mean values of soil temperature, PST, outgoing shortwave radiation, and TT were greater than in 2008 …

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Fresh Produce As A Potential Vector And Reservoir For Human Bacterial Pathogens: Revealing The Ambiguity Of Interaction And Transmission, Ahmed Esmael, Rashad R. Al-Hindi, Raed S. Albiheyri, Mona G. Alharbi, Amani A. R. Filimban, Mazen S. Alseghayer, Abdulaziz M. Almaneea, Meshari Ahmed Alhadlaq, Jumaa Ayubu, Addisu D. Teklemariam Mar 2023

Fresh Produce As A Potential Vector And Reservoir For Human Bacterial Pathogens: Revealing The Ambiguity Of Interaction And Transmission, Ahmed Esmael, Rashad R. Al-Hindi, Raed S. Albiheyri, Mona G. Alharbi, Amani A. R. Filimban, Mazen S. Alseghayer, Abdulaziz M. Almaneea, Meshari Ahmed Alhadlaq, Jumaa Ayubu, Addisu D. Teklemariam

Nebraska Center for Virology: Faculty Publications

The consumer demand for fresh produce (vegetables and fruits) has considerably increased since the 1980s for more nutritious foods and healthier life practices, particularly in developed countries. Currently, several foodborne outbreaks have been linked to fresh produce. The global rise in fresh produce associated with human infections may be due to the use of wastewater or any contaminated water for the cultivation of fruits and vegetables, the firm attachment of the foodborne pathogens on the plant surface, and the internalization of these agents deep inside the tissue of the plant, poor disinfection practices and human consumption of raw fresh produce. …

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Spoilage Pseudomonas Survive Common Thermal Processing Schedules And Grow In Emulsified Meat During Extended Vacuum Storage, Samuel C. Watson, Rebecca A. Furbeck, Samodha C. Fernando, Byron D. Chaves, Gary Sullivan Mar 2023

Spoilage Pseudomonas Survive Common Thermal Processing Schedules And Grow In Emulsified Meat During Extended Vacuum Storage, Samuel C. Watson, Rebecca A. Furbeck, Samodha C. Fernando, Byron D. Chaves, Gary Sullivan

Department of Animal Science: Faculty Publications

Some Pseudomonas species are common meat spoilage bacteria that are often associated with the spoilage of fresh meat. The recently reported ability of these bacteria to also spoil cooked and vacuum packaged meat products has created the need to investigate all potential routes of spoilage they may be able to utilize. The objective of this experiment was to determine if spoilage Pseudomonas spp. survive thermal processing and grow during refrigerated storage under vacuum. Pseudomonas spp. isolates collected from spoiled turkey products were inoculated into a salted and seasoned meat emulsion that was vacuum sealed and thermally treated to final temperatures …

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Functional Annotation Of The Animal Genomes: An Integrated Annotation Resource For The Horse, Sichong Peng, Anna R. Dahlgren, Callum G. Donnelly, Erin N. Hales, Jessica Lynn Petersen, Rebecca R. Bellone, Ted Kalbfleisch, Carrie J. Finno Mar 2023

Functional Annotation Of The Animal Genomes: An Integrated Annotation Resource For The Horse, Sichong Peng, Anna R. Dahlgren, Callum G. Donnelly, Erin N. Hales, Jessica Lynn Petersen, Rebecca R. Bellone, Ted Kalbfleisch, Carrie J. Finno

Department of Animal Science: Faculty Publications

The genomic sequence of the horse has been available since 2009, providing critical resources for discovering important genomic variants regarding both animal health and population structures. However, to fully understand the functional implications of these variants, detailed annotation of the horse genome is required. Due to the limited availability of functional data for the equine genome, as well as the technical limitations of short-read RNAseq, existing annotation of the equine genome contains limited information about important aspects of gene regulation, such as alternate isoforms and regulatory elements, which are either not transcribed or transcribed at a very low level. To …

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Comparison Of Two Diagnostic Assays For The Detection Of Serum Neutralizing Antibody To Porcine Epidemic Diarrhea Virus, Justin Brown, Korakrit Poonsuk, Ting-Yu Cheng, Chris Rademacher, Erin Kalkwarf, Liying Tian, Lauren A. Mckeen, Chong Wang, Luis Gimenez-Lirola, Gimenez-Lirola Baum, Locke A. Karriker Feb 2023

Comparison Of Two Diagnostic Assays For The Detection Of Serum Neutralizing Antibody To Porcine Epidemic Diarrhea Virus, Justin Brown, Korakrit Poonsuk, Ting-Yu Cheng, Chris Rademacher, Erin Kalkwarf, Liying Tian, Lauren A. Mckeen, Chong Wang, Luis Gimenez-Lirola, Gimenez-Lirola Baum, Locke A. Karriker

Nebraska Center for Virology: Faculty Publications

Lactogenic immunity is important for the protection of piglets against many pathogens including porcine epidemic diarrhea virus. Circulating neutralizing antibodies levels in sow sera may help determine if a detectable immune response could confer protection to piglets. Neutralizing antibodies can be detected through various diagnostic assays. This study evaluated the diagnostic characteristics of two neutralizing antibody assays for porcine epidemic diarrhea virus neutralizing antibodies in serum of challenged gilts. Four treatment groups, control, non-vaccinated, vaccinated prior to challenge, and vaccinated following challenge, were comprised of 20 gilts. Serum sample were collected from each gilt prior to and following challenge with …

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Was Kaposi’S Sarcoma-Associated Herpesvirus Introduced Into China Via The Ancient Silk Road? An Evolutionary Perspective, Zhenqiu Liu, Qiwen Fang, Jialu Zuo, Veenu Minhas, Charles Wood, Na He, Tiejun Zhang Feb 2023

Was Kaposi’S Sarcoma-Associated Herpesvirus Introduced Into China Via The Ancient Silk Road? An Evolutionary Perspective, Zhenqiu Liu, Qiwen Fang, Jialu Zuo, Veenu Minhas, Charles Wood, Na He, Tiejun Zhang

Nebraska Center for Virology: Faculty Publications

Kaposi’s sarcoma-associated herpesvirus (KSHV) has become widely dispersed worldwide since it was first reported in 1994, but the seroprevalence of KSHV varies geographically. KSHV is relatively ubiquitous in Mediterranean areas and the Xinjiang Uygur Autonomous Region, China. The origin of KSHV has long been puzzling. In the present study, we collected and analysed 154 KSHV ORF-K1 sequences obtained from samples originating from Xinjiang, Italy, Greece, Iran and southern Siberia using Bayesian evolutionary analysis in BEAST to test the hypothesis that KSHV was introduced into Xinjiang via the ancient Silk Road. According to the phylogenetic analysis, 72 sequences were subtype A …

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Journal Of Dairy Science® 2022 Editorial Report, Paul J. Kononoff Feb 2023

Journal Of Dairy Science® 2022 Editorial Report, Paul J. Kononoff

Department of Animal Science: Faculty Publications

In 2022, the 105th volume of the Journal of Dairy Science was published. As we review the journal’s last year, it may be useful to reflect on the purpose of scientific publishing. This can be simply stated as the dissemination of results that extends knowledge to open the door for new concepts or advances in the field (Chan, 2018). Similar to other journals, in addition to original research articles, Journal of Dairy Science also publishes invited reviews and other paper types to pursue this purpose. The journal also has other valuable roles. For example, scientific journals actively support authors in …

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Periconceptual Maternal Nutrition Affects Fetal Liver Programming Of Energy- And Lipid-Related Genes, Wellison J. S. Diniz, Alison K. Ward, Kacie L. Mccathy, Cierrah J. Kassetas, Friederike Baumgaertner, Lawrence P. Reynolds, Pawel P. Borowicz, Kevin K. Sedivec, James D. Kirsch, Sheri T. Dorsam, Tammi L. Neville, J. Chris Forcherio, Ronald Scott, Joel S. Caton, Carl R. Dahlen Feb 2023

Periconceptual Maternal Nutrition Affects Fetal Liver Programming Of Energy- And Lipid-Related Genes, Wellison J. S. Diniz, Alison K. Ward, Kacie L. Mccathy, Cierrah J. Kassetas, Friederike Baumgaertner, Lawrence P. Reynolds, Pawel P. Borowicz, Kevin K. Sedivec, James D. Kirsch, Sheri T. Dorsam, Tammi L. Neville, J. Chris Forcherio, Ronald Scott, Joel S. Caton, Carl R. Dahlen

Department of Animal Science: Faculty Publications

During pregnancy, the fetus relies on the dam for its nutrient supply. Nutritional stimuli during fetal organ development can program hepatic metabolism and function. Herein, we investigated the role of vitamin and mineral supplementation (VTM or NoVTM—at least 71 days pre-breeding to day 83 of gestation) and rate of weight gain (low (LG) or moderate (MG)—from breeding to day 83) on the fetal liver transcriptome and the underlying biological pathways. Crossbred Angus beef heifers (n = 35) were randomly assigned to one of four treatments in a 2 × 2 factorial design (VTM_LG, VTM_MG, NoVTM_LG, and NoVTM_MG). Gene expression …

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