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Articles 181 - 210 of 295

Full-Text Articles in Forest Sciences

The Motion Of Trees In The Wind: A Data Synthesis, Toby D. Jackson, Sarab Sethi, Ebba Dellwik, Nikolas Angelou, Amanda Bunce, Tim Van Emmerik, Mark Rudnicki, Et. Al. Jul 2021

The Motion Of Trees In The Wind: A Data Synthesis, Toby D. Jackson, Sarab Sethi, Ebba Dellwik, Nikolas Angelou, Amanda Bunce, Tim Van Emmerik, Mark Rudnicki, Et. Al.

Michigan Tech Publications, Part 1

Interactions between wind and trees control energy exchanges between the atmosphere and forest canopies. This energy exchange can lead to the widespread damage of trees, and wind is a key disturbance agent in many of the world's forests. However, most research on this topic has focused on conifer plantations, where risk management is economically important, rather than broadleaf forests, which dominate the forest carbon cycle. This study brings together tree motion time-series data to systematically evaluate the factors influencing tree responses to wind loading, including data from both broadleaf and coniferous trees in forests and open environments. We found that …


A Systems Biology Approach Identifies A Regulator, Bplerf1, Of Cold Tolerance In Betula Platyphylla, Kaiwen Lv, Wenqi Wu, Hairong Wei, Guifeng Liu Jun 2021

A Systems Biology Approach Identifies A Regulator, Bplerf1, Of Cold Tolerance In Betula Platyphylla, Kaiwen Lv, Wenqi Wu, Hairong Wei, Guifeng Liu

Michigan Tech Publications, Part 1

Cold is an abiotic stress that can greatly affect the growth and survival of plants. Here, we reported that an AP2/ERF family gene, BplERF1, isolated from Betula platyphylla played a contributing role in cold stress tolerance. Overexpression of BplERF1 in B. platyphylla transgenic lines enhanced cold stress tolerance by increasing the scavenging capability and reducing H2O2 and malondialdehyde (MDA) content in transgenic plants. Construction of BplERF-mediated multilayered hierarchical gene regulatory network (ML-hGRN), using Top-down GGM algorithm and the transcriptomic data of BplERF1 overexpression lines, led to the identification of five candidate target genes of BplERF1 which include MPK20, …


Bel1-Like Homeodomain Protein Blh6a Is A Negative Regulator Of Cal5h2 In Sinapyl Alcohol Monolignol Biosynthesis In Poplar, Qiao Wang, Xinren Dai, Hongying Pang, Yanxia Cheng, Xiong Huang, Hui Li, Hairong Wei, Et. Al. Jun 2021

Bel1-Like Homeodomain Protein Blh6a Is A Negative Regulator Of Cal5h2 In Sinapyl Alcohol Monolignol Biosynthesis In Poplar, Qiao Wang, Xinren Dai, Hongying Pang, Yanxia Cheng, Xiong Huang, Hui Li, Hairong Wei, Et. Al.

Michigan Tech Publications, Part 1

Lignin is one of the major components of xylem cell walls in tree stems. The lignin in the wood of most flowering plants (dicotyledonous angiosperms) is typically polymerized from three monolignol precursors, coniferyl alcohol, sinapyl alcohol, and p-coumaroyl alcohol, resulting in guaiacyl (G), syringyl (S), and hydroxyphenyl (H) subunits, respectively. In this study, we focus on the transcriptional regulation of a coniferaldehyde 5-hydroxylase (CAld5H2) gene, which encodes a key enzyme for sinapyl alcohol biosynthesis. We carried out a yeast one-hybrid (Y1H) screen to identify candidate upstream transcription factors (TFs) regulating CAld5H2. We obtained 12 upstream TFs …


Successful Management Of Invasive Rats Across A Fragmented Landscape, Sarah K. Barney, Devin R. Leopold, Kainana Francisco, David J. Flaspohler, Tadashi Fukami, Christian P. Giardina, Daniel S. Gruner, Jessie L. Knowlton, William C. Pitt, Erin E. Wilson Rankin Jun 2021

Successful Management Of Invasive Rats Across A Fragmented Landscape, Sarah K. Barney, Devin R. Leopold, Kainana Francisco, David J. Flaspohler, Tadashi Fukami, Christian P. Giardina, Daniel S. Gruner, Jessie L. Knowlton, William C. Pitt, Erin E. Wilson Rankin

Michigan Tech Publications, Part 1

Summary Introduced mammalian predators are responsible for the decline and extinction of many native species, with rats (genus Rattus) being among the most widespread and damaging invaders worldwide. In a naturally fragmented landscape, we demonstrate the multi-year effectiveness of snap traps in the removal of Rattus rattus and Rattus exulans from lava-surrounded forest fragments ranging in size from 10 ha. Relative to other studies, we observed low levels of fragment recolonization. Larger rats were the first to be trapped, with the average size of trapped rats decreasing over time. Rat removal led to distinct shifts in the foraging height and …


Chromosome-Level Genome Assembly Of A Regenerable Maize Inbred Line A188, Guifang Lin, Cheng He, Jun Zheng, Dal Hoe Koo, Ha Le, Huakun Zheng, Hairong Wei, Et. Al. Jun 2021

Chromosome-Level Genome Assembly Of A Regenerable Maize Inbred Line A188, Guifang Lin, Cheng He, Jun Zheng, Dal Hoe Koo, Ha Le, Huakun Zheng, Hairong Wei, Et. Al.

Michigan Tech Publications, Part 1

Background: The maize inbred line A188 is an attractive model for elucidation of gene function and improvement due to its high embryogenic capacity and many contrasting traits to the first maize reference genome, B73, and other elite lines. The lack of a genome assembly of A188 limits its use as a model for functional studies. Results: Here, we present a chromosome-level genome assembly of A188 using long reads and optical maps. Comparison of A188 with B73 using both whole-genome alignments and read depths from sequencing reads identify approximately 1.1 Gb of syntenic sequences as well as extensive structural variation, including …


Coarse Woody Debris Decomposition Assessment Tool: Model Development And Sensitivity Analysis, Zhaohua Dai, Carl C. Trettin, Andrew Burton, Martin Jurgensen, Deborah S. Page-Dumroese, Brian T. Forschler, Jonathan S. Schilling, Daniel L. Lindner Jun 2021

Coarse Woody Debris Decomposition Assessment Tool: Model Development And Sensitivity Analysis, Zhaohua Dai, Carl C. Trettin, Andrew Burton, Martin Jurgensen, Deborah S. Page-Dumroese, Brian T. Forschler, Jonathan S. Schilling, Daniel L. Lindner

Michigan Tech Publications, Part 1

Coarse woody debris (CWD) is an important component in forests, hosting a variety of organisms that have critical roles in nutrient cycling and carbon (C) storage. We developed a process-based model using literature, field observations, and expert knowledge to assess woody debris decomposition in forests and the movement of wood C into the soil and atmosphere. The sensitivity analysis was conducted against the primary ecological drivers (wood properties and ambient conditions) used as model inputs. The analysis used eighty-nine climate datasets from North America, from tropical (14.2° N) to boreal (65.0° N) zones, with large ranges in annual mean temperature …


Transcriptome-Wide Identification And Characterization Of Micrornas In Diverse Phases Of Wood Formation In Populus Trichocarpa, Ruiqi Wang, Mengxuan Reng, Shuanghui Tian, Cong Liu, He Cheng, Yingying Liu, Hairong Wei, Et. Al. Jun 2021

Transcriptome-Wide Identification And Characterization Of Micrornas In Diverse Phases Of Wood Formation In Populus Trichocarpa, Ruiqi Wang, Mengxuan Reng, Shuanghui Tian, Cong Liu, He Cheng, Yingying Liu, Hairong Wei, Et. Al.

Michigan Tech Publications, Part 1

We applied miRNA expression profiling method to Populus trichocarpa stems of the three developmental stages, primary stem (PS), transitional stem (TS), and secondary stem (SS), to investigate miRNA species and their regulation on lignocellulosic synthesis and related processes. We obtained 892, 872, and 882 known miRNAs and 1727, 1723, and 1597 novel miRNAs, from PS, TS, and SS, respectively. Comparisons of these miRNA species among different developmental stages led to the identification of 114, 306, and 152 differentially expressed miRNAs (DE-miRNAs), which had 921, 2639, and 2042 candidate target genes (CTGs) in the three respective stages of the same order. …


The Diverse Roles Of Cytokinins In Regulating Leaf Development, Wenqi Wu, Kang Du, Xiangyang Kang, Hairong Wei Jun 2021

The Diverse Roles Of Cytokinins In Regulating Leaf Development, Wenqi Wu, Kang Du, Xiangyang Kang, Hairong Wei

Michigan Tech Publications, Part 1

Leaves provide energy for plants, and consequently for animals, through photosynthesis. Despite their important functions, plant leaf developmental processes and their underlying mechanisms have not been well characterized. Here, we provide a holistic description of leaf developmental processes that is centered on cytokinins and their signaling functions. Cytokinins maintain the growth potential (pluripotency) of shoot apical meristems, which provide stem cells for the generation of leaf primordia during the initial stage of leaf formation; cytokinins and auxins, as well as their interaction, determine the phyllotaxis pattern. The activities of cytokinins in various regions of the leaf, especially at the margins, …


Beyond Planning Tools: Experiential Learning In Climate Adaptation Planning And Practices, Kristen M. Schmitt, Todd A. Ontl, Stephen D. Handler, Maria K. Janowiak, Leslie A. Brandt, Patricia Butler-Leopold, P. Danielle Shannon, Courtney L. Peterson, Christopher W. Swanston May 2021

Beyond Planning Tools: Experiential Learning In Climate Adaptation Planning And Practices, Kristen M. Schmitt, Todd A. Ontl, Stephen D. Handler, Maria K. Janowiak, Leslie A. Brandt, Patricia Butler-Leopold, P. Danielle Shannon, Courtney L. Peterson, Christopher W. Swanston

Michigan Tech Publications, Part 1

In the past decade, several dedicated tools have been developed to help natural resources professionals integrate climate science into their planning and implementation; however, it is unclear how often these tools lead to on-the-ground climate adaptation. Here, we describe a training approach that we developed to help managers effectively plan to execute intentional, climate-informed actions. This training approach was developed through the Climate Change Response Framework (CCRF) and uses active and focused work time and peer-to-peer interaction to overcome observed barriers to using adaptation planning tools. We evaluate the effectiveness of this approach by examining participant evaluations and outlining the …


Measuring Dynamic Changes In The Spatial Pattern And Connectivity Of Surface Waters Based On Landscape And Graph Metrics: A Case Study Of Henan Province In Central China, Bo Mu, Guohang Tian, Gengyu Xin, Miao Hu, Panpan Yang, Yiwen Wang, Hao Xie, Audrey L. Mayer, Yali Zhang May 2021

Measuring Dynamic Changes In The Spatial Pattern And Connectivity Of Surface Waters Based On Landscape And Graph Metrics: A Case Study Of Henan Province In Central China, Bo Mu, Guohang Tian, Gengyu Xin, Miao Hu, Panpan Yang, Yiwen Wang, Hao Xie, Audrey L. Mayer, Yali Zhang

Michigan Tech Publications, Part 1

An understanding of the scientific layout of surface water space is crucial for the sustainable development of human society and the ecological environment. The objective of this study was to use land-use/land-cover data to identify the spatiotemporal dynamic change processes and the influencing factors over the past three decades in Henan Province, central China. Multidisciplinary theories (landscape ecology and graph theory) and methods (GIS spatial analysis and SPSS correlation analysis) were used to quantify the dynamic changes in surface water pattern and connectivity. Our results revealed that the water area decreased significantly during the periods of 1990–2000 and 2010–2018 due …


Research Inequity In The Plant Sciences, Theresa M. Culley, Megan Philpott, Robert Tunison, Benjamin J. Merritt, José M. Barreiro Sanchez, Alexis Wafer, Rebecca Holdren Apr 2021

Research Inequity In The Plant Sciences, Theresa M. Culley, Megan Philpott, Robert Tunison, Benjamin J. Merritt, José M. Barreiro Sanchez, Alexis Wafer, Rebecca Holdren

Michigan Tech Publications, Part 1

Do all plant biologists worldwide have equal access to novel methods, enabling them to be equally productive, publish, and receive credit for their research? Or does reduced access to cutting-edge techniques in countries with lower financial resources create an inequity for researchers located there? Such disparities and biases do exist within our discipline and must be addressed if we are to move forward as a more just society. Applications in Plant Sciences has taken steps to address this important issue of research inequity, as outlined below. We now call upon the entire botanical community—researchers, editors and reviewers, funding agencies, and …


Comparing Genetic Diversity In Three Threatened Oaks, Emma Suzuki Spence, Jeremie B. Fant, Oliver Gailing, M. Patrick Griffith, Kayri Havens, Andrew L. Hipp, Priyanka Dipak Kadav, Andrea Kramer, Patrick Thompson, Raakel Toppila, Murphy Westwood, Jordan Wood, Bethany A. Zumwalde, Sean Hoban Apr 2021

Comparing Genetic Diversity In Three Threatened Oaks, Emma Suzuki Spence, Jeremie B. Fant, Oliver Gailing, M. Patrick Griffith, Kayri Havens, Andrew L. Hipp, Priyanka Dipak Kadav, Andrea Kramer, Patrick Thompson, Raakel Toppila, Murphy Westwood, Jordan Wood, Bethany A. Zumwalde, Sean Hoban

Michigan Tech Publications, Part 1

Genetic diversity is a critical resource for species’ survival during times of environmental change. Conserving and sustainably managing genetic diversity requires understanding the distribution and amount of genetic diversity (in situ and ex situ) across multiple species. This paper focuses on three emblematic and IUCN Red List threatened oaks (Quercus, Fagaceae), a highly speciose tree genus that contains numerous rare species and poses challenges for ex situ conservation. We compare the genetic diversity of three rare oak species-Quercus georgiana, Q. oglethorpensis, and Q. boyntonii-to common oaks; investigate the correlation of range size, population size, and the abiotic environment with genetic …


Intended And Unintended Consequences Of Wolf Restoration To Yellowstone And Isle Royale National Parks, Douglas W. Smith, Rolf O. Peterson Apr 2021

Intended And Unintended Consequences Of Wolf Restoration To Yellowstone And Isle Royale National Parks, Douglas W. Smith, Rolf O. Peterson

Michigan Tech Publications, Part 1

Wolves (Canis lupus), a once widely distributed species, were systematically removed from many temperate zone ecosystems due to conflicts with humans. A change in human attitudes and cultural norms has brought about a recovery in some suitable areas, yet reintroductions are still controversial. Two notable reintroduction areas in the United States were Yellowstone and Isle Royale National Parks. Both proposals caused polarization and debate. In Yellowstone opposition focused on outside the park effects, mainly wolves killing livestock and wild game also desired by human hunters. At Isle Royale, opposition was mostly about human interventions into nature and impairment of wilderness …


Hb-Pls: A Statistical Method For Identifying Biological Process Or Pathway Regulators By Integrating Huber Loss And Berhu Penalty With Partial Least Squares Regression, Wenping Deng, Kui Zhang, Cheng He, Sanzhen Liu, Hairong Wei Mar 2021

Hb-Pls: A Statistical Method For Identifying Biological Process Or Pathway Regulators By Integrating Huber Loss And Berhu Penalty With Partial Least Squares Regression, Wenping Deng, Kui Zhang, Cheng He, Sanzhen Liu, Hairong Wei

Michigan Tech Publications, Part 1

Gene expression data features high dimensionality, multicollinearity, and non-Gaussian distribution noise, posing hurdles for identification of true regulatory genes controlling a biological process or pathway. In this study, we integrated the Huber loss function and the Berhu penalty (HB) into partial least squares (PLS) framework to deal with the high dimension and multicollinearity property of gene expression data, and developed a new method called HB-PLS regression to model the relationships between regulatory genes and pathway genes. To solve the Huber-Berhu optimization problem, an accelerated proximal gradient descent algorithm with at least 10 times faster than the general convex optimization solver …


Finding Purpose In The Conservation Of Biodiversity By The Commingling Of Science And Ethics, John A. Vucetich, Ewan A. Macdonald, Dawn Burnham, Jeremy T. Bruskotter, Dominic D.P. Johnson, David W. Macdonald Mar 2021

Finding Purpose In The Conservation Of Biodiversity By The Commingling Of Science And Ethics, John A. Vucetich, Ewan A. Macdonald, Dawn Burnham, Jeremy T. Bruskotter, Dominic D.P. Johnson, David W. Macdonald

Michigan Tech Publications, Part 1

Averting the biodiversity crisis requires closing a gap between how humans tend to behave, individually and collectively, and how we ought to behave—“ought to” in the sense of behaviors required to avert the biodiversity crisis. Closing that gap requires synthesizing insight from ethics with insights from social and behavioral sciences. This article contributes to that synthesis, which presents in several provocative hypotheses: (i) Lessening the biodiversity crisis requires promoting pro-conservation behavior among humans. Doing so requires better scientific understanding of how one’s sense of purpose in life affects conservation-relevant behaviors. Psychology and virtue-focused ethics indicate that behavior is importantly influenced …


Bird Communities In African Cocoa Agroforestry Are Diverse But Lack Specialized Insectivores, Crinan Jarrett, Thomas B. Smith, Tabe T.R. Claire, Diogo F. Ferreira, Melanie Tchoumbou, Malange N.F. Elikwo, Jared D. Wolfe, Kristin Brzeski, Andreanna J. Welch, Rachid Hanna, Luke L. Powell Mar 2021

Bird Communities In African Cocoa Agroforestry Are Diverse But Lack Specialized Insectivores, Crinan Jarrett, Thomas B. Smith, Tabe T.R. Claire, Diogo F. Ferreira, Melanie Tchoumbou, Malange N.F. Elikwo, Jared D. Wolfe, Kristin Brzeski, Andreanna J. Welch, Rachid Hanna, Luke L. Powell

Michigan Tech Publications, Part 1

Forests are being converted to agriculture throughout the Afrotropics, driving declines in sensitive rainforest taxa such as understorey birds. The ongoing expansion of cocoa agriculture, a common small-scale farming commodity, has contributed to the loss of 80% rainforest cover in some African countries. African cocoa farms may provide habitat for biodiversity, yet little is known about their suitability for vertebrate fauna, or the effect of farm management on animal communities. Here, we report the first in-depth investigation into avian diversity and community composition in African cocoa, by assembling a dataset of 9,566 individual birds caught across 83 sites over 30 …


Birds And Bioenergy Within The Americas: A Cross‐National, Social–Ecological Study Of Ecosystem Service Tradeoffs, Jessie L. Knowlton, Kathleen E. Halvorsen, David J. Flaspohler, Christopher R. Webster, Jesse Abrams, Sara M. Almeida, Stefan L. Arriaga‐Weiss, Brad Barnett, Maíra R. Cardoso, Pablo V. Cerqueira, Diana Córdoba, Marcos Persio Dantas‐Santos, Jennifer L. Dunn, Amarella Eastmond, Gina M. Jarvi, Julian A. Licata, Ena Mata‐Zayas, Rodrigo Medeiros, M. Azahara Mesa‐Jurado, Lízbeth Yamily Moo‐Culebro, Cassandra Moseley, Erik Nielsen, Colin Phifer, Erin Pischke, Chelsea Schelly, Theresa Selfa, Chelsea A. Silva, Tatiana Souza, Sam R. Sweitz Mar 2021

Birds And Bioenergy Within The Americas: A Cross‐National, Social–Ecological Study Of Ecosystem Service Tradeoffs, Jessie L. Knowlton, Kathleen E. Halvorsen, David J. Flaspohler, Christopher R. Webster, Jesse Abrams, Sara M. Almeida, Stefan L. Arriaga‐Weiss, Brad Barnett, Maíra R. Cardoso, Pablo V. Cerqueira, Diana Córdoba, Marcos Persio Dantas‐Santos, Jennifer L. Dunn, Amarella Eastmond, Gina M. Jarvi, Julian A. Licata, Ena Mata‐Zayas, Rodrigo Medeiros, M. Azahara Mesa‐Jurado, Lízbeth Yamily Moo‐Culebro, Cassandra Moseley, Erik Nielsen, Colin Phifer, Erin Pischke, Chelsea Schelly, Theresa Selfa, Chelsea A. Silva, Tatiana Souza, Sam R. Sweitz

Michigan Tech Publications, Part 1

Although renewable energy holds great promise in mitigating climate change, there are socioeconomic and ecological tradeoffs related to each form of renewable energy. Forest‐related bioenergy is especially controversial, because tree plantations often replace land that could be used to grow food crops and can have negative impacts on biodiversity. In this study, we examined public perceptions and ecosystem service tradeoffs between the provisioning services associated with cover types associated with bioenergy crop (feedstock) production and forest habitat‐related supporting services for birds, which themselves provide cultural and regulating services. We combined a social survey‐based assessment of local values and perceptions with …


Early Bud-Break 1 And Early Bud-Break 3 Control Resumption Of Poplar Growth After Winter Dormancy, Abdul Azeez, Yiru Chen Zhao, Rajesh Kumar Singh, Yordan S. Yordanov, Madhumita Dash, Pal Miskolczi, Katja Stojkovic, Steven H. Strauss, Rishikesh P. Bhalerao, Victor Busov Feb 2021

Early Bud-Break 1 And Early Bud-Break 3 Control Resumption Of Poplar Growth After Winter Dormancy, Abdul Azeez, Yiru Chen Zhao, Rajesh Kumar Singh, Yordan S. Yordanov, Madhumita Dash, Pal Miskolczi, Katja Stojkovic, Steven H. Strauss, Rishikesh P. Bhalerao, Victor Busov

Michigan Tech Publications, Part 1

Bud-break is an economically and environmentally important process in trees and shrubs from boreal and temperate latitudes, but its molecular mechanisms are poorly understood. Here, we show that two previously reported transcription factors, EARLY BUD BREAK 1 (EBB1) and SHORT VEGETATIVE PHASE-Like (SVL) directly interact to control bud-break. EBB1 is a positive regulator of bud-break, whereas SVL is a negative regulator of bud-break. EBB1 directly and negatively regulates SVL expression. We further report the identification and characterization of the EBB3 gene. EBB3 is a temperature-responsive, epigenetically-regulated, positive regulator of bud-break that provides a direct link to activation of the cell …


Thinking Big And Thinking Small: A Conceptual Framework For Best Practices In Community And Stakeholder Engagement In Food, Energy, And Water Systems, Andrew Kliskey, Paula Williams, David L. Griffith, Virginia H. Dale, Chelsea Schelly, Anna Maria Marshall, Valoree Gagnon, Weston M. Eaton, Kristin Floress Feb 2021

Thinking Big And Thinking Small: A Conceptual Framework For Best Practices In Community And Stakeholder Engagement In Food, Energy, And Water Systems, Andrew Kliskey, Paula Williams, David L. Griffith, Virginia H. Dale, Chelsea Schelly, Anna Maria Marshall, Valoree Gagnon, Weston M. Eaton, Kristin Floress

Michigan Tech Publications, Part 1

Community and stakeholder engagement is increasingly recognized as essential to science at the nexus of food, energy, and water systems (FEWS) to address complex issues surrounding food and energy production and water provision for society. Yet no comprehensive framework exists for supporting best practices in community and stakeholder engagement for FEWS. A review and meta‐synthesis were undertaken of a broad range of existing models, frameworks, and toolkits for community and stakeholder engagement. A framework is proposed that comprises situational awareness of the FEWS place or problem, creation of a suitable culture for engagement, focus on power‐sharing in the engagement process, …


Genome Sequence And Evolution Of Betula Platyphylla, Su Chen, Yucheng Wang, Lili Yu, Tao Zheng, Sui Wang, Zhen Yue, Sapna Kumari, Hairong Wei, Et. Al. Feb 2021

Genome Sequence And Evolution Of Betula Platyphylla, Su Chen, Yucheng Wang, Lili Yu, Tao Zheng, Sui Wang, Zhen Yue, Sapna Kumari, Hairong Wei, Et. Al.

Michigan Tech Publications, Part 1

Betula L. (birch) is a pioneer hardwood tree species with ecological, economic, and evolutionary importance in the Northern Hemisphere. We sequenced the Betula platyphylla genome and assembled the sequences into 14 chromosomes. The Betula genome lacks evidence of recent whole-genome duplication and has the same paleoploidy level as Vitis vinifera and Prunus mume. Phylogenetic analysis of lignin pathway genes coupled with tissue-specific expression patterns provided clues for understanding the formation of higher ratios of syringyl to guaiacyl lignin observed in Betula species. Our transcriptome analysis of leaf tissues under a time-series cold stress experiment revealed the presence of the MEKK1–MKK2–MPK4 …


Bacterium-Enabled Transient Gene Activation By Artificial Transcription Factor For Resolving Gene Regulation In Maize, Mingxia Zhao, Zhao Peng, Yang Qin, Ling Zhang, Bin Tian, Yueying Chen, Hairong Wei, Et. Al. Feb 2021

Bacterium-Enabled Transient Gene Activation By Artificial Transcription Factor For Resolving Gene Regulation In Maize, Mingxia Zhao, Zhao Peng, Yang Qin, Ling Zhang, Bin Tian, Yueying Chen, Hairong Wei, Et. Al.

Michigan Tech Publications, Part 1

Cellular functions are diversified through intricate transcription regulations, and an understanding gene regulation networks is essential to elucidating many developmental processes and environmental responses. Here, we employed the Transcriptional-Activator Like effectors (TALes), which represent a family of transcription factors that are synthesized by members of the γ-proteobacterium genus Xanthomonas and secreted to host cells for activation of targeted host genes. Through delivery by the maize pathogen, Xanthomonas vasicola pv. vasculorum, designer TALes (dTALes), which are synthetic TALes, were used to induce the expression of the maize gene glossy3 (gl3), a MYB transcription factor gene involved in the …


Microrna319-Mediated Gene Regulatory Network Impacts Leaf Development And Morphogenesis In Poplar, Yanxia Cheng, Lihu Wang, Manzar Abbas, Xiong Huang, Qiao Wang, Aimin Wu, Hairong Wei, Et. Al. Feb 2021

Microrna319-Mediated Gene Regulatory Network Impacts Leaf Development And Morphogenesis In Poplar, Yanxia Cheng, Lihu Wang, Manzar Abbas, Xiong Huang, Qiao Wang, Aimin Wu, Hairong Wei, Et. Al.

Michigan Tech Publications, Part 1

MicroRNA319 (miR319) has been implicated in leaf development in a number of plant species. Here we study the roles of miR319a and its regulated network in leaf development in poplars. Over-expression of miR319a in Populus alba × Populus glandulosa caused dwarf statures, narrow leaf blades and serrated leaf margins. The vascular bundles and bundle sheaths in transgenic leaves had more layers of cells than those in the leaves of control plants, indicating enhanced lignification in these cells. Among the 93 putative targets of miR319a predicted with the psRNATarget tool, only three genes, TCP (TEOSINTE BRANCHED1, CYCLOIDEA, and …


Consequences Of Increased Variation In Peatland Hydrology For Carbon Storage: Legacy Effects Of Drought And Flood In A Boreal Fen Ecosystem, Evan Kane, Catherine M. Dieleman, Danielle L. Rupp, Kevin H. Wyatt Feb 2021

Consequences Of Increased Variation In Peatland Hydrology For Carbon Storage: Legacy Effects Of Drought And Flood In A Boreal Fen Ecosystem, Evan Kane, Catherine M. Dieleman, Danielle L. Rupp, Kevin H. Wyatt

Michigan Tech Publications, Part 1

Globally important carbon (C) stores in boreal peatlands are vulnerable to altered hydrology through changes in precipitation and runoff patterns, groundwater inputs, and a changing cryosphere. These changes can affect the extent of boreal wetlands and their ability to sequester and transform C and other nutrients. Variation in precipitation patterns has also been increasing, with greater occurrences of both flooding and drought periods. Recent work has pointed to the increasing role of algal production in regulating C cycling during flooded periods in fen peatlands, but exactly how this affects the C sink-strength of these ecosystems is poorly understood. We evaluated …


Inaugural Editorial, Hairong Wei Jan 2021

Inaugural Editorial, Hairong Wei

Michigan Tech Publications, Part 1

No abstract provided.


Growth-Regulating Factor 5 (Grf5)-Mediated Gene Regulatory Network Promotes Leaf Growth And Expansion In Poplar, Wenqi Wu, Jiang Li, Qiao Wang, Kaiwen Lv, Kang Du, Hairong Wei Jan 2021

Growth-Regulating Factor 5 (Grf5)-Mediated Gene Regulatory Network Promotes Leaf Growth And Expansion In Poplar, Wenqi Wu, Jiang Li, Qiao Wang, Kaiwen Lv, Kang Du, Hairong Wei

Michigan Tech Publications, Part 1

Although polyploid plants have larger leaves than their diploid counterparts, the molecular mechanisms underlying this difference (or trait) remain elusive. Differentially expressed genes (DEGs) between triploid and full-sib diploid poplar trees were identified from two transcriptomic data sets followed by a gene association study among DEGs to identify key leaf growth regulators. Yeast one-hybrid system, electrophoretic mobility shift assay, and dual-luciferase assay were employed to substantiate that PpnGRF5-1 directly regulated PpnCKX1. The interactions between PpnGRF5-1 and growth-regulating factor (GRF)-interacting factors (GIFs) were experimentally validated and a multilayered hierarchical regulatory network (ML-hGRN)-mediated by PpnGRF5-1 was constructed with top-down graphic Gaussian model …


Root Biomass And Production By Two Cushion Plant Species Of Tropical High-Elevation Peatlands In The Andean Páramo, Esteban Suárez, Segundo Chimbolema, Rodney Chimner, Erik A. Lilleskov Jan 2021

Root Biomass And Production By Two Cushion Plant Species Of Tropical High-Elevation Peatlands In The Andean Páramo, Esteban Suárez, Segundo Chimbolema, Rodney Chimner, Erik A. Lilleskov

Michigan Tech Publications, Part 1

High-elevation peatlands in the Andes are receiving increasing attention for their biodiversity and their high rates of carbon accumulation. However, the ecology of these peatlands and the environmental factors that control their carbon dynamics remain under-studied. Here we report on the patterns of root biomass productivity and turnover rates for two cushion plant species (Distichia muscoides, Plantago rigida) that commonly dominate high-elevation peatlands (> 4200 m a.s.l.) in the Andean páramo landscape of Northern Ecuador. Root biomass for P. rigida ranged from 680 to 864 g m-2 and was approximately 40 % higher than for D. muscoides (507–620 g m-2). …


Fine-Scale Movements And Behaviors Of Coyotes (Canis Latrans) During Their Reproductive Period, Michael J. Chamberlain, Bradley S. Cohen, Patrick H. Wightman, Emily Rushton, Joseph W. Hinton Jan 2021

Fine-Scale Movements And Behaviors Of Coyotes (Canis Latrans) During Their Reproductive Period, Michael J. Chamberlain, Bradley S. Cohen, Patrick H. Wightman, Emily Rushton, Joseph W. Hinton

Michigan Tech Publications, Part 1

In canids, resident breeders hold territories but require different resources than transient individuals (i.e., dispersers), which may result in differential use of space, land cover, and food by residents and transients. In the southeastern United States, coyote (Canis latrans) reproduction occurs during spring and is energetically demanding for residents, but transients do not reproduce and therefore can exhibit feeding behaviors with lower energetic rewards. Hence, how coyotes behave in their environment likely differs between resident and transient coyotes. We captured and monitored 36 coyotes in Georgia during 2018–2019 and used data from 11 resident breeders, 12 predispersing residents (i.e., offspring …


High-Quality De Novo Assembly Of The Eucommia Ulmoides Haploid Genome Provides New Insights Into Evolution And Rubber Biosynthesis, Yun Li, Hairong Wei, Jun Yang, Kang Du, Jiang Li, Ying Zhang, Tong Qiu, Zhao Liu, Yongyu Ren, Lianjun Song, Xiangyang Kang Dec 2020

High-Quality De Novo Assembly Of The Eucommia Ulmoides Haploid Genome Provides New Insights Into Evolution And Rubber Biosynthesis, Yun Li, Hairong Wei, Jun Yang, Kang Du, Jiang Li, Ying Zhang, Tong Qiu, Zhao Liu, Yongyu Ren, Lianjun Song, Xiangyang Kang

Michigan Tech Publications, Part 1

We report the acquisition of a high-quality haploid chromosome-scale genome assembly for the first time in a tree species, Eucommia ulmoides, which is known for its rubber biosynthesis and medicinal applications. The assembly was obtained by applying PacBio and Hi–C technologies to a haploid that we specifically generated. Compared to the initial genome release, this one has significantly improved assembly quality. The scaffold N50 (53.15 MB) increased 28-fold, and the repetitive sequence content (520 Mb) increased by 158.24 Mb, whereas the number of gaps decreased from 104,772 to 128. A total of 92.87% of the 26,001 predicted protein-coding genes identified …


High-Purity Recycling Of Hematite And Zn/Cu Mixture From Waste Smelting Slag, Yang Huo, Xiang Song, Suiyi Zhu, Yu Chen, Xue Lin, Yaqiong Wu, Zhan Qu, Ting Su, Xinfeng Xie Dec 2020

High-Purity Recycling Of Hematite And Zn/Cu Mixture From Waste Smelting Slag, Yang Huo, Xiang Song, Suiyi Zhu, Yu Chen, Xue Lin, Yaqiong Wu, Zhan Qu, Ting Su, Xinfeng Xie

Michigan Tech Publications, Part 1

In this study, Zn/Cu-bearing smelting slag was recycled via an integrated acid dissolution and hematite precipitation method. The slag was dissolved in nitric acid to generate an acid solution containing 23.5 g/L Fe, 4.45 g/L Zn and 2.81 g/L Cu, which was subjected to hydrothermal treatment with the addition of levulinic acid (LA). More than 99.95% of the initial Fe content was removed as hematite particles with diameters of approximately 200 nm, and the residual Fe concentration in the acid was 0.43 mg/L. The generated hematite contained 97.3% Fe2O3, 0.64% ZnO and 0.58% CuO. Greater than 99% of the initial …


Comparative Proteomic Analysis Of Leaves At Different Ages In Allotriploid Populus, Jiang Li, Yi Wang, Hairong Wei, Xiangyang Kang Nov 2020

Comparative Proteomic Analysis Of Leaves At Different Ages In Allotriploid Populus, Jiang Li, Yi Wang, Hairong Wei, Xiangyang Kang

Michigan Tech Publications, Part 1

Triploid poplar trees have been shown to have a number of growth advantages, especially much bigger leaves that contribute greatly to the increased biomass. In this study, we focused on the relationships between leaf age and leaf metabolism in triploids. We performed comparative proteomic analysis of the 5th (FDR5), 10th (FDR10), and 25th (FDR25) leaves from the apical meristems in allotriploids originated from first-division restitution (FDR). A total of 1970, 1916, and 1850 proteins were identified in the FDR5, FDR10, and FDR25, respectively. Principle component analysis (PCA) and differentially accumulated protein (DAP) analysis showed that FDR10 and FDR25 displayed higher …