Forest Sciences Commons^™

Nitrogen Fertilization, Stand Age, And Overstory Tree Species Impact The Herbaceous Layer In A Central Appalachian Hardwood Forest, Lacey J. Smith, Kirsten Stephan

Faculty & Staff Scholarship

Research Highlights: Herb-layer community composition, abundance, species richness, and Shannon–Wiener diversity index are shaped by nitrogen fertilization, disturbance history, and the overstory tree species in its immediate vicinity. Background and Objectives: While the herbaceous layer in deciduous forests is increasingly recognized for its importance in various aspects of forest ecosystem function, this study sought to describe the factors impacting the herbaceous layer. Specifically, this study’s objective was to quantify and compare herb-layer species composition, cover, and other community indices in watersheds with (a) different levels of N deposition, (b) different stand ages due to differing disturbance histories, and (c) different …

Sustainability And Economics Of White Oak (Quercus Alba) Timber Supply In Kentucky, Gaurav Dhungel

Theses and Dissertations--Forestry and Natural Resources

Sustained timber supply relies upon balanced forest age distribution, where the level of harvesting and mortality is compensated by regeneration and growth among younger age classes. However, current forest inventory trends from the white oak growing region reveal sustainability threat from declining white oak regeneration and recruitment leading to disproportionate inventory structure. Consequently, there exists growing concern among stakeholders on long-term sustainability of white oak timber and its economic implications, particularly in Kentucky, because of the significance of the species to the state’s economy. This research aims to examine past and current inventory levels of white oak in Kentucky using …

The Impacts Of Imazapic On Garlic Mustard And Non-Target Forest Floor Vegetation In Central Kentucky’S Hardwood Forests, Pavan Kumar Podapati

Theses and Dissertations--Forestry and Natural Resources

Alliaria petiolata is an invasive biennial herb that poses a substantial threat to various ecosystems across the United States. Imazapic herbicide can control A. petiolata infestations, but there is limited peer-reviewed data on impacts of pre-emergent imazapic spraying to forest floor communities. This research examined the impacts of pre-emergent imazapic (0.84 kg/ha) with Pentra-Bark® surfactant on ground cover of A. petiolata and the spring perennials Claytonia virginica and Erigenia bulbosa. Experimental populations in randomized blocks within two forest stands in central Kentucky received the following treatments at 0.84 kg ai/ha: imazapic with Pentra-Bark®, glyphosate with Pentra-Bark®, Pentra-Bark® alone, and …

Southern Pine Beetle-Specific Rna Interference Demonstrates No Effects On Nontarget Insects, Hannah Marie Hollowell

Theses and Dissertations--Entomology

The endemic southern pine beetle (SPB), Dendroctonus frontalis Zimmermann, is a highly destructive bark beetle that has devastated coniferous forests of the southern United States for decades. Traditional management strategies have been unable to respond effectively to largescale SPB outbreaks, prompting exploration of novel techniques including RNA interference (RNAi). Double-stranded RNAs (dsRNAs) are effective at triggering gene silencing and inducing mortality in SPB, but determining delivery methods and demonstrating specificity of the treatments is necessary before this technology may progress to deployment.

I evaluated three SPB-specific dsRNAs on model nontarget insects to assess potential lethal effects, sublethal effects such as …

Environmental Data: Geospatial And Elevation Data, Albert J. Burky, Julia I. Chapman, Ryan W. Mcewan

Data Files: Drew Woods Permanent Plots

GPS Coordinates, Elevation, and Distance to Edge data for 32 sample plots in Drew Woods State Nature Preserve in Darke County, OH. Data key is provided in a supplemental file.

Seedling Growth And Soil Chemistry In A Chronosequence Of Forest Restoration From Agriculture, Michaela J. Woods, Sarah J. Frankenberg, Joseph R. Juodvalkis, Mary C. Lloyd, Meredith Cobb, Ryan Mcewan

Five Rivers MetroParks Collaboration Data Archive

Soils were collected from forest restorations that were 0, 7, 10, 50 and 100 years old and assessed for soil nutrients, chemistry, and enzymatic activity. Quercus macrocarpa seedlings were planted in each soil type, after 5 months of growth seedlings were destructively harvested. Seedling mass and the colonization rate of their roots by ectomycorrhizal fungi are reported.

The photo accompanying this data collection is of the researchers' germinating Quercus macrocarpa seeds.

White Pine Blister Rust Distribution In New Hampshire 1900-2018: Exploring The Impacts Of An Exotic Pathogen On Forest Composition And Succession, Janine Marr

Antioch University Dissertations & Theses

White pine blister rust (WPBR) has been affecting New Hampshire’s white pines for more than a century, yet no data exist on the long-term effects of the non-native disease on the state’s forests, particularly with respect to the regeneration and sustainability of white pine, and forest succession. This study aimed to address the gaps in the literature by exploring: 1) the current distribution, incidence, and severity of WPBR in New Hampshire; 2) the application of two historical hazard ratings models, one climatic, and one biotic; and 3) the long-term effects of the disease on forest composition, structure, and succession. Historical …

Effectiveness Of Hybrid Micro-Learning For Financial Education Of Filipino Women Migrants In Italy, Manuela Prandini, Pier Paolo Ficarelli

Journal of Public Affairs and Development

Faced with the dramatic changes effected by the COVID-19 pandemic, adult education has to capitalize on new learning and teaching paradigms. In recent years, technology-based micro-learning has grown into an established educational approach. Its focus is on short, well-defined units with the use of interactive multimedia available on digital channels. The flexibility of self-regulated learning through bite-sized tasks combined with synchronous virtual sessions for sharing and discussion can be a game-changer for non-formal adult education, capable of overcoming participants’ reduced time availability and the complications of face-to-face exchanges at the present time. Using a mixed-method approach, this action research study …

Perceived Stressors Of Filipinos In South Korea And The Development Of An Appreciative Resilience-Focused Wellness Program, Monica H. Walet, Emely D. Dicolen, Joeffrey M. Calimag

Journal of Public Affairs and Development

People are faced with numerous challenges brought by the COVID-19 pandemic. It has affected all the areas of their well-being - physical, psycho-emotional, social, financial, and spiritual. Overseas Filipinos, specifically the Filipinos in South Korea, are not exempted from these. It is in this light that this research analyzed the factors that affect the well-being of Filipinos in South Korea and propose a sustainable program that would provide the needed support to navigate their unique challenges and perceived requirements for personal growth. A total of 353 Filipinos from various sojourns participated in the survey: Employment Permit System workers, marriage migrants, …

Academe-Led Community Organizing In The Philippines’S Economically Disadvantaged Areas: The Case Of Science And Technology Community-Based For Inclusive Development (Stc4id) Program, Emilia S. Visco, Maria Emilinda T. Mendoza, Diana A. Torio, Dhino B. Geges, Clarice C. Pulumbarit, Jennifer Marie S. Amparo, Ron Jay P. Dangcalan, Regina Karla J. Gache, April Charmaine C. Soltura, Lorie M. Alborida, King Niño B. Manzano

Journal of Public Affairs and Development

Part of the mandate of educational institutions, particularly State Colleges and Universities (SUCs) is to provide various forms of community, public, and volunteer services that contribute to the country’s social and economic development, hence the emergence of extension programs facilitated by the academe. Considered a novel concept is the academe-led participatory extension program such as the DOST-PCAARRD’s Science and Technology Community-Based for Inclusive Development (STC4iD) Program. This program introduces new community organizing (CO) perspectives in the geographically, economically, and socially disadvantaged areas (GESDA) of the Philippines. Hence, there is much to be learned about how academe-led CO can be instrumental …

Collaborative Conservation And Backcountry Weed Control: A Case Study Of The Great Burn Proposed Wilderness, Christopher James Prange

Graduate Student Theses, Dissertations, & Professional Papers

Noxious weeds have become a management priority on public lands in the United States. Managing invasive weed populations in natural areas with complex ecosystems presents issues that need a systems-wide approach over long temporal and spatial scales. This broad-scale problem increasingly demands collaborative efforts. While collaborative conservation has become a tool in natural resource management during the 21^st century, it is less commonly applied in weed control in backcountry wilderness areas. Programs that have been initiated are understudied. Accordingly, this research was conducted through semi-structured interviews to gather perspectives of weed professionals involved in a collaborative backcountry weed program …

Sula Study Revisited: 20-Year Post-Fire Regeneration In The Southern Bitterroot Valley, Montana., Luke Alan Rymniak

Graduate Student Theses, Dissertations, & Professional Papers

In the summer of 2000, a number of large fires burned in the southern Bitterroot Valley near Sula, Montana. Research was conducted in 2001 and 2003 in the fire-affected areas of the French Basin and Larid Creek areas in order to investigate the effects of environmental variables, fire severity, and post-fire management on vegetation regeneration. In 2020 these areas were remeasured to understand trends over time by evaluating the impact of these same factors 20 years post fire. The results showed that the effects of environmental variables, fire severity, and post-fire management on vegetation regeneration were varied. The most influential …

Seed Oil Content And Fatty Acid Profiles Of Endemic Phoenix Theophrasti Greuter, Phoenixroebelenii O'Brien, Phoenix Caneriensis Hort. Ex Chabaud, And Phoenix Dactylifera L.Grown In The Same Locality In Turkey, Zeynep Ergün

Turkish Journal of Agriculture and Forestry

Plants-based oils and fats usually obtained from seeds have been indispensable substances for mankind, both in nutritional and industrial respect. This study aimed to determine the total amount of oil and the fatty acid compositions of Phoenix theophrasti Greuter, P. roebelenii O'Brien, P. caneriensis Hort. Ex Chabaud, and P. dactylifera L. The seeds of these four Phoenix species were collected from the same location, east Mediterranean agroecological conditions when the fruits were fully ripened, which means the environmental factors that may affect the oil content and the fatty acid compositions were controlled. The highest oil content of the samples was …

Influence Of Different Drying Methods And Cold Storage Treatments On The Postharvestquality And Nutritional Properties Of P. Ostreatus Mushroom, Beyhan Ki̇bar

Turkish Journal of Agriculture and Forestry

This study was conducted to determine the effects of different drying methods and cold storage treatments on the postharvest quality and nutritional properties of P. ostreatus mushroom. In the study, six different treatments including natural drying, oven drying (60 °C), microwave drying (450 W), storage at -18 °C, 4 °C and 10 °C were evaluated. After drying and cold storage, pH value, ash, protein, color, total phenolic content, total flavonoid content, antioxidant activity and element contents in mushroom samples were detected. According to findings obtained from the study, drying and cold storage treatments significantly (p < 0.01) affected the postharvest quality and nutritional composition of P. ostreatus. The protein content, ash content, L* color value, total phenolic content, total flavonoid content, antioxidant activity and essential mineral contents such as potassium, magnesium, phosphorus, calcium, iron, zinc, and manganese of P. ostreatus decreased after drying and cold storage compared to the fresh mushroom sample. As a result, the best treatment was determined as storage at 4 °C in terms of protection the quality and nutritional value of P. ostreatus during postharvest storage. In addition, natural drying was the most effective treatment among drying methods, and it can be used successfully for longterm storage of this mushroom. However, it was concluded that microwave drying method was not suitable for the preservation of P. ostreatus since it caused significant nutrient and quality losses. The results of the present study will provide useful information about postharvest preservation and processing of P. ostreatus for researchers working on this issue.

Effects Of Biological Metabolism Of Metasequoia Glyptostroboides On Nutrient Elementcontent And Enzyme Activity In Seedling Soil, Cuiyue Dong

Turkish Journal of Agriculture and Forestry

Soil has high spatial variability. Under the change of water level, soil nutrient element content and enzyme activity do not show regular changes. Therefore, the effects of biological metabolism on soil nutrient element content and enzyme activity in the adult stage of Metasequoia glyptostroboides were proposed. The characteristics of the soil were extracted, the irreversibility, accumulation, concealment and refractory rationality of the nutrient element content in the soil were expounded. According to the soil characteristics, the relationship between the nutrient element content and enzyme activity in the soil was analysed. The pH value, organic matter, lead and manganese contents of …

Acceleration Of Growth In Tomato Seedlings Grown With Growth Retardant, Ufuk Uçan, Atnan Uğur

Turkish Journal of Agriculture and Forestry

In vegetable growing, seedling is the foundation of a successful growing. Use of commercial seedlings have become more popular in recent years due to effective use of labour and land and economic concerns. It is aimed to produce small vigorous seedlings with sufficient roots and free of diseases and pests in the commercial seedling enterprises. Although certain cultural applications are used for production of small-sized seedlings, growth retarding chemicals are generally preferred due to their effective results. There are some concerns regarding on-going retarding effects of such chemicals under field conditions. In this study, it is aimed to remove the …

Optimization Of Agrobacterium-Mediated Transformation And Regeneration For Crispr/Cas9 Genome Editing Of Commercial Tomato Cultivars, Zafer Seçgi̇n, Musa Kavas, Kubi̇lay Yildirim

Turkish Journal of Agriculture and Forestry

Tomato (Solanum lycopersicum) is the second most important horticultural crop worldwide that is widely used as a model plant in genetic manipulation of Solanaceae. CRISPR/Cas9 system has been successfully utilized in several studies for genome edition of model tomato cultivars. However, these genome editing systems should be also optimized for commercial tomato cultivar for direct application of genome editing in field conditions. In this study, we have optimized an Agrobacterium-mediated gene transfer and regeneration system for CRISPR/Cas9 genome editing in two commercial tomato cultivars for the first time. The effect of explant type, genotype, pre-transformation time, Agrobacterium concentration, infection time, …

Influences On Conifer Drought Responses In Northern California, Wallis L. Robinson

Cal Poly Humboldt theses and projects

California is experiencing increasingly severe and prolonged droughts, which are contributing to changes in tree stress and forest mortality. Many factors affect a tree’s drought response, including competition, climate, and site and tree characteristics. Northern California provides a suitable venue to explore the effects of these factors, as it spans a variety of site conditions and includes habitat for conifers with different adaptations and requirements. This study used annual ¹³C discrimination and growth metrics to assess differences in drought resistance and resilience in conifers adapted to coastal and montane ranges at both wet and dry sites, as well as …

A Spatiotemporal Assessment Of Fish Assemblage Response To Land-Use Change And The Evaluation Of Edna Metabarcoding For Describing Diverse Fish Communities, Timothy M. Owen

Theses and Dissertations

Fish assemblages are often assessed as a biological proxy for environmental health. While humans value healthy environments for the ecosystem services and recreational opportunities they provide, it is increasingly evident that such resources can be paradoxically degraded by anthropogenic activities. In this investigation, we studied the relationship between different intensities of anthropogenic land-use change and habitat-driven fish assemblage response across multiple spatiotemporal scales. Secondarily, we explored the efficacy of eDNA metabarcoding against conventional electrofishing techniques for the purpose of describing complete fish communities. This study was conducted in the Tuckahoe Creek basin near Richmond, Virginia. This James River tributary serves …

Use Of Redwood Basal Hollows By Bats: A Focus On The Townsend's Big-Eared Bat On The North Coast Of California, Amon Jotesh Armstrong

Cal Poly Humboldt theses and projects

Loss of roosting resources, either through disturbance or removal, negatively affects bats. For sensitive species, such as the Townsend’s big-eared bat (Corynorhinus townsendii), determining roost requirements is a critical component in conserving their habitat. Such cavity roosting bats on the North Coast of California may use hollows in large redwood trees. In this study, I examined the factors determining the use of basal tree hollows by different bat species at eight redwood forest sites in Del Norte, Humboldt, and Mendocino Counties, California. Bat guano was collected from 179 basal hollow roosts from 2017 to 2018, and guano mass …

Recreation, Vegetation Management, And Disease Impact Sympatric Carnivore Activity In California's East Bay Parks, Leigh J. Douglas

Cal Poly Humboldt theses and projects

East Bay Regional Park District designated over 1000 ha of protected wildland-urban interface habitat in the hills of California’s East Bay Area for invasive tree removal to reduce fire risk and restore native habitat over a 10-year period starting in 2016. From June to November 2019, 36 camera traps were deployed using a stratified two-pronged detection approach of surveying recreation and wildlife trails to assess the impact of vegetation management on the spatiotemporal distribution of sympatric carnivore species while accounting for potential impacts of human activity and proximity to development. The sampling effort resulted in 5,191 cumulative trap nights, 2,739 …

Post-Fire Effects In Chaparral And Oak Ecosystems Of Northern California, Abigail M. Jones

Cal Poly Humboldt theses and projects

The issue of wildfires, hazard fuels management, and post-fire tree mortality has become an increasingly common topic in the western United States. This thesis is composed of two studies, with the first study, Chapter 1, examining fuel treatment effectiveness and the second study, Chapter 2, striving to characterize post-fire mortality in oaks.

Prior to wildfire, fuel reduction projects may take place to decrease the likelihood of high severity fire around human infrastructure and communities. Within California’s chaparral ecosystems, common treatment types include hand-thinning, prescribed burning, mechanical mastication, and mechanical mastication followed with prescribed burning. Because chaparral has a longer historical …

Identification Of Genetic Basis Associated With Agronomic Traits In A Global Safflower Panelusing Genome-Wide Association Study, Fawad Ali, Muhammad Azhar Nadeem, Ephrem Habyarimana, Muhammad Tanveer Altaf, Muzaffer Barut, Cemal Kurt, Hassan Javed Chaudhary, Iftikhar Hussain Khalil, Mehtap Yildiz, Gönül Cömertpay, Muhammad Qasim Shahid, Faheem Shahzad Baloch

Turkish Journal of Agriculture and Forestry

Safflower is an underutilized and minor oilseed crop that received less attention from the scientific community compared to other oilseed crops like soybean and sunflower. Exploring the genetic basis associated with agronomic traits is crucial for markerassisted breeding of safflower. A genome-wide association study was conducted using a total of 12,232 DArTseq markers to identify the marker-trait association for important agronomic traits in an international safflower panel derived from 26 different geographical countries of the world. Statistically significant genotypic effects (p < 0.05) were observed across mean data of both locations (Pakistan and Turkey). Moderate to high heritability estimates were observed for the studied traits. Studied material showed higher performance for all traits except seeds per capitulum in Pakistan compared to Turkey. Phenotypic diversity for important agronomic traits, such as plant height (60.08 to 121.48 cm), capitula per plant (8.7 to 80.4), seeds per capitulum (15 to 42.05), and seed yield per plant (4.85 to 51.02 g), was illustrated. Seed yield per plant showed a highly significant and positive correlation with capitula per plant (0.4985***). Constellation plot analysis resulted in four groups, i.e. A, B, C, and D. Genotyping by sequencing resulted in 12,232 DArTseq markers being used for the investigation of marker-trait association through mixed linear model (Q + K) approach. DArT-38077549 showed significant association with capitula per plant, while two markers (DArT-22763576, DArT-22763253) were associated with plant height. A total of two markers (DArT-38079422, DArT-100043360) were associated with seeds per capitulum. A total of five DArTseq markers showed significant association with seed yield per plant and maximum variation was resulted by DArT-100004992. The results of this study provide a new insight to understand the genetic basis associated with agronomic traits in safflower. We envisage that significant markers identified through this investigation may be applicable in future safflower marker-assisted breeding programs.

Genetic Characterization Of Almond (Prunus Amygdalus L) Using Microsatellite Markersin The Area Of Adriatic Sea, Jasna Hasanbegovic, Semina Hadziabulic, Mirsad Kurtovic, Fuad Gasi, Biljana Lazovic, Boris Dorbic, Azra Skender

Turkish Journal of Agriculture and Forestry

The use of microsatellite (SSR) markers has successfully found its application in genetic characterization and examination of the origin of a large number of fruit species. Mediterranean germplasm is characterized by a great variety of almond genotypes. The study covered two geographically distant regions Montenegro (Bar) and Croatia (Sibenik) in a sample of 60 almond genotypes. Genetic analysis of almonds involved the use of ten microsatellite primers for genetic characterization of 60 examined genotypes, which successfully amplified PCR products and were highly polymorphic. Nine microsatellite markers used for the genetic characterization of almonds are derived from Prunus persica (UDP97-402, UDP98-411, …

Comparison Of Phenotypic And Marker-Assisted Selection In Turkish Cultivars And Global Genotypes Of Chickpea For Resistance To Pathotypes Of Ascochyta Rabiei (Pass.) Labr., Hüseyi̇n Kabakci, Göksel Özer

Turkish Journal of Agriculture and Forestry

The pathotypes of Ascochyta rabiei, which causes ascochyta blight, show a high pathogenic variation. Forty-four chickpeas, including 26 cultivars commonly cultivated in Turkey and 18 global genotypes, were characterized for resistance status to ascochyta blight following inoculation with four pathotypes of A. Rabiei. The pathogenicity experiments were conducted using the whole-plant inoculation method and completely randomized designs with three replicates. The pathogenicity tests revealed that 32, 17, 3, and 1 chickpea showed resistance reactions to pathotypes I, II, III, and IV, respectively. These chickpeas were genotyped with STMS (GAA47, TA146, and TA194), SCAR (SCK13603, SCAE19336, and SCY17590), and an allele-specific …

Effects Of 1-Methylcyclopropene (1-Mcp) Treatment On Antioxidant Enzymes And Fruit Quality Parameters Of Cold-Stored Baby Squash, Mehmet Seçki̇n Kurubaş, Jasmina Sabotic, Mustafa Erkan

Turkish Journal of Agriculture and Forestry

In this study, the effects of 1-methylcyclopropene (1-MCP) treatment on the antioxidant enzymes and fruit quality parameters of baby squash (Cucurbita pepo L., cv. Soleil F1 and İskender F1) during cold storage was investigated. After being freshly harvested, the İskender F1 (green-skinned) and Soleil F1 (yellow-skinned) baby squash were treated with 250 and 500 ppb concentrations of 1-MCP and stored at 10 °C for 14 days. The 250 ppb 1-MCP treatment suppressed the loss of total soluble solid, ascorbic acid, total phenolic, and total flavonoid contents during storage in the Soleil F1 squash. Similarly, the 250 ppb 1-MCP treatment delayed …

Comparative Analysis Of The Relationship Between Morphological, Physiological, And Biochemical Properties In Spinach (Spinacea Oleracea L.) Under Deficit Irrigation Conditions, Musa Seymen

Turkish Journal of Agriculture and Forestry

Drought is an abiotic stress factor that negatively affects plant development and causes economic losses in plant production, and its effect is getting significant every day. Spinach contains a high amount of water and drought stress affects its development as well as causing changes in its biochemical contents. In this study, it is aimed to determine the changes in the morphological, physiological, and biochemical contents of spinach grown under deficit irrigation conditions in different stages of development. For this purpose, by considering five different irrigation levels (I100, I80, I60, I40, and I20), and 4 harvest times determined according to the …

The Potential Of Göktürk 2 Satellite Images For Mapping Burnt Forest Areas, Murat Kuruca, Di̇lek Küçük Matci, Uğur Avdan

Turkish Journal of Agriculture and Forestry

Using remotely sensed data to identify burnt forest areas produces fast, economical, and highly accurate results. Accordingly, in this study we investigate the capabilities of Göktürk-2, Turkey's national satellite, for mapping burnt forest areas. We compare our results with those obtained from Landsat-8 and Worldview-2 satellite images, which are frequently used for mapping burnt areas. The capabilities of the satellites are compared, in terms of detecting burnt forest areas, using support vector machine (SVM) and rotation forest (RF) classification, which are advanced methods. According to the results of the accuracy analysis, SVM classification gives similar kappa statistics and overall accuracy …

Differences Of Flavonoid Structural Genes Preferentially Expressed In Brown And Green Natural Colored Cotton, Öner Canavar, Mark D. Rausher

Turkish Journal of Agriculture and Forestry

We compared the expression levels of some structural genes involved in the flavonoid pathway between two brown cotton lines (brown 16 and light brown 14) and two green cotton lines (greenish 12 and light green 5). Gene expression levels of six structural genes F3'h (flavonoid 3'-hydroxylase), F3'5'h (flavonoid 3'5'-hydroxylase), Dfr (dihydroflavonol 4-reductase), Lar (leucoanthocyanidin reductase), Ans (anthocyanidin synthase), and Anr (anthocyanidin reductase) were all substantially highly expressed in both brown cotton lines than in green cotton lines. Our study also revealed differences in expression levels between the two brown cotton lines. F3'h and F3'5'h had higher expression in the brown …

Rapid And Efficient In Vitro Regeneration Of Transplastomic Potato (Solanum Tuberosum L.) Plants After Particle Bombardment, Md Jakir Hossain, Emre Aksoy, Zahi̇de Nesli̇han Öztürk Gökçe, Faiz Ahmad Joyia, Muhammad Sarwar Khan, Allah Bakhsh

Turkish Journal of Agriculture and Forestry

In contrast with traditional nuclear gene transformation, transplastomic technology has opened a new horizon for the transgenic plant research that offers several beneficial aspects including the convenient use of transgene stacking and the generation of high expression levels of foreign proteins. However, this technology has been well adopted and established in tobacco, the introduction and adoption of cost-effective, swift, and reproducible protocol for in vitro regeneration of transplastomic potato is challenging and laborious. The present research aimed to develop such prompt and efficient protocol to instigate and revive the regeneration potential with the combinations of different plant growth regulators (PGRs). …