Forest Sciences Commons^™

Single-Cell Transcriptome And Network Analyses Unveil Key Transcription Factors Regulating Mesophyll Cell Development In Maize, Shentong Tao, Peng Liu, Yining Shi, Yilong Feng, Jingjing Gao, Lifen Chen, Hairong Wei, Et, Al,

Michigan Tech Publications

BACKGROUND: Maize mesophyll (M) cells play important roles in various biological processes such as photosynthesis II and secondary metabolism. Functional differentiation occurs during M-cell development, but the underlying mechanisms for regulating M-cell development are largely unknown. RESULTS: We conducted single-cell RNA sequencing (scRNA-seq) to profile transcripts in maize leaves. We then identified coregulated modules by analyzing the resulting pseudo-time-series data through gene regulatory network analyses. , , , and () families were highly expressed in the early stage, whereas () and families were highly expressed in the late stage of M-cell development. Construction of regulatory networks revealed that these transcript …