Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Forest Sciences
Single-Cell Transcriptome And Network Analyses Unveil Key Transcription Factors Regulating Mesophyll Cell Development In Maize, Shentong Tao, Peng Liu, Yining Shi, Yilong Feng, Jingjing Gao, Lifen Chen, Hairong Wei, Et, Al,
Single-Cell Transcriptome And Network Analyses Unveil Key Transcription Factors Regulating Mesophyll Cell Development In Maize, Shentong Tao, Peng Liu, Yining Shi, Yilong Feng, Jingjing Gao, Lifen Chen, Hairong Wei, Et, Al,
Michigan Tech Publications
BACKGROUND: Maize mesophyll (M) cells play important roles in various biological processes such as photosynthesis II and secondary metabolism. Functional differentiation occurs during M-cell development, but the underlying mechanisms for regulating M-cell development are largely unknown. RESULTS: We conducted single-cell RNA sequencing (scRNA-seq) to profile transcripts in maize leaves. We then identified coregulated modules by analyzing the resulting pseudo-time-series data through gene regulatory network analyses. , , , and () families were highly expressed in the early stage, whereas () and families were highly expressed in the late stage of M-cell development. Construction of regulatory networks revealed that these transcript …
Chromosome-Level Genome Assembly Of A Regenerable Maize Inbred Line A188, Guifang Lin, Cheng He, Jun Zheng, Dal Hoe Koo, Ha Le, Huakun Zheng, Hairong Wei, Et. Al.
Chromosome-Level Genome Assembly Of A Regenerable Maize Inbred Line A188, Guifang Lin, Cheng He, Jun Zheng, Dal Hoe Koo, Ha Le, Huakun Zheng, Hairong Wei, Et. Al.
Michigan Tech Publications
Background: The maize inbred line A188 is an attractive model for elucidation of gene function and improvement due to its high embryogenic capacity and many contrasting traits to the first maize reference genome, B73, and other elite lines. The lack of a genome assembly of A188 limits its use as a model for functional studies. Results: Here, we present a chromosome-level genome assembly of A188 using long reads and optical maps. Comparison of A188 with B73 using both whole-genome alignments and read depths from sequencing reads identify approximately 1.1 Gb of syntenic sequences as well as extensive structural variation, including …