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2015

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Full-Text Articles in Food Processing

Study On Process Conditions Of Superfine Grinding Modifying Insoluble Dietary Fiber From Potato Pulp, Zhang Gensheng, Ge Yingliang, Nie Zhiqiang, Yue Xiaoxia, Han Bing, Cheng Jianbo Dec 2015

Study On Process Conditions Of Superfine Grinding Modifying Insoluble Dietary Fiber From Potato Pulp, Zhang Gensheng, Ge Yingliang, Nie Zhiqiang, Yue Xiaoxia, Han Bing, Cheng Jianbo

Food and Machinery

Insoluble dietary fiber of potato slag was chosen to modify with superfine grinding using colloid mill. Inter-tooth space,of colloid mill, material-to-liquid ratio, treatment time are optimized by single-factor and orthogonal designed experiment and are defined with solubility process. Results shown optimum modified conditions of colloid mill: inter-tooth space 8.00 μm, 3 000 r/min for 5 min, material-to-liquid ratio 1∶60. Under these conditions, the solubility is 18.33%.


Study On Combined Drying Technology Of Lotus Root Chips, Xing Fang, Wang Jianhui, He Xinyi, Wu Haiqing, Wu Jiali, Liu Pei Dec 2015

Study On Combined Drying Technology Of Lotus Root Chips, Xing Fang, Wang Jianhui, He Xinyi, Wu Haiqing, Wu Jiali, Liu Pei

Food and Machinery

To obtain the the processing technology of lotus root chips, taken lotus root as raw materials, the effects offreeze drying time, hot-air drying temperature and hot-air drying time on quality of lotus chips by combination of freeze drying and hot-air drying were explored. Experiment results indicated the optimized technological parameters of combined freeze dring and hot-air drying were freeze drying 4h, then hot-air drying at 50 ℃ for 2 h. At those conditions, the color value L*, a*, b* and hardness value of lotus root slices were 77.65, 0.96, 9.03 and 385.3 g, respectively. The results showed that combined drying …


Influence Of Modified Attapulgite On Non-Biological Stability Of Beer, Shi Jinglue Dec 2015

Influence Of Modified Attapulgite On Non-Biological Stability Of Beer, Shi Jinglue

Food and Machinery

Report a study on the modification conditions of attapulgite towards improving its adsorption affinity and specificity of the chill haze protein in beer. Quantity of attapulgite added and adsorption time used were optimized using univariate and multivariate analysis with orthogonal parameter sets. Experiments conducted were focusing on the non-biological stability, foam stability and filtration performance of beer. Adsorption specificity test of the modified attapulgite with the chill haze protein in beer was also presented. An optimal condition for the modification was concluded that the attapulgite be heated at 303 ℃ for 2.54 h. With a quantity of 0.87 g/L added …


Brewing Process Optimization Of Gordon Euryale Seed Wine, Li Yan, Lin Meiying, Li Yunsong Dec 2015

Brewing Process Optimization Of Gordon Euryale Seed Wine, Li Yan, Lin Meiying, Li Yunsong

Food and Machinery

The gordon euryale seed starch was hydrolyzed by α-amylase and glucoamylase to prepare starch syrup, and then the saccharomyces cerevisiae was added to prepare the health wine. The technology parameters were optimized by single factor and orthogonal experiments. And the optimal fermentation conditions were gotten. With the ratio of mass to water of 1︰10, the optimum liquefaction conditions were: addition of α-amylase of 0.4%, liquefaction temperature 70 ℃, and liquefaction time 60 min. The optimum saccharifying conditions were: addition of glucoamylase 0.5%, saccharifying temperature 60 ℃,and saccharifying time of 1 h. Under the saccharifying conditions, the sugar content of the …


Optimization On Processing Of Trachinotus Ovatus Surimi Gel By Response Surface Methodology, Huang He, Wang Na, Chen Liang, Wu Wenlong Dec 2015

Optimization On Processing Of Trachinotus Ovatus Surimi Gel By Response Surface Methodology, Huang He, Wang Na, Chen Liang, Wu Wenlong

Food and Machinery

With Trachinotus ovatus surimi gel as the research object, using the fractional factorial experiment screened out five factors from the seven factors, such as rinsing time, rinsing times, mincing time and twice heating time, which had a great effect on the surimi gel strength and whiteness. And then through the steepest uphill test to approach the maximum response area of the 5 factors, the final optimized by central composite design response surface methodology, the optimal results showed that: the rinsing time was 3 min, rinsing 5 times, mincing time was 21 min, the first heating time was 10 min, second …


Application Of Extrusion Technology For Preparing Defatted Black Soybean Meal Reconstituted Powder, Zhang Lin, Li Jianke, Ren Caixia, Sun Xuesi, Qin Lingxiang Dec 2015

Application Of Extrusion Technology For Preparing Defatted Black Soybean Meal Reconstituted Powder, Zhang Lin, Li Jianke, Ren Caixia, Sun Xuesi, Qin Lingxiang

Food and Machinery

The extruding and expanding experiment was studied with the black soybean meal as material, mixing corn starch by twin-screw extruder to make reconstituted powder, The best expanded technical parameters were determined, and studied the formula, the extrusion ratio, the reconstituability, the viscosity, the color of this powder flour. The optimal formula of the powder was as follows: black soybean meal 30%, corn starch 45%, sugar 15%, maltodextrin 10%, creamer 6%.


Inactivation Of Escherichia Coli O157:H7 And Shiga Toxin Producing E. Coli (Stec) Throughout Beef Summer Sausage Production And The Use Of High Pressure Processing As An Alternative Intervention To Thermal Processing, Eric L. Oliver Dec 2015

Inactivation Of Escherichia Coli O157:H7 And Shiga Toxin Producing E. Coli (Stec) Throughout Beef Summer Sausage Production And The Use Of High Pressure Processing As An Alternative Intervention To Thermal Processing, Eric L. Oliver

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Escherichia coli O157:H7 and six additional serogroups of Shiga Toxin-producing E. coli (STEC-7) have been implicated in foodborne illness outbreaks related to fermented sausage products and are considered adulterants by the USDA-FSIS. The objective of this study was to evaluate high pressure processing (HPP) as an alternative to thermal treatment to reduce STEC-7 meeting the USDA-FSIS performance standards (5.0-log reduction). A rifampicin-resistant, non-pathogenic E. coli (surrogate) cocktail, was used in manufacturing testing. Sausages were fermented at ~42.2°C, slowly increased to 54.4°C internal temperature, cold showered, and chilled for 6 hours. Sampling occurred post-fermentation (PF; pH 5.0), internal temperature 48.9°C (I-48.9°C), …


Impacts Of Feedstock And Parboiling Conditions On Quality Characteristics Of Parboiled Rice, Kanokporn Leethanapanich Dec 2015

Impacts Of Feedstock And Parboiling Conditions On Quality Characteristics Of Parboiled Rice, Kanokporn Leethanapanich

Graduate Theses and Dissertations

Parboiling involves soaking, steaming, and drying, and is known to improve head rice yield (HRY), remove chalkiness, and retain nutrients. Because of an increasing number of rice cultivars developed, commingling of rice may happen during harvesting, distributing, or storage. Using commingled rice with a wide range of gelatinization temperatures (To) as a feedstock may cause inconsistent parboiled rice products. Therefore, this study was aimed at investigating the effect of soaking temperature and steaming duration on the quality of parboiled, commingled rice. Rough rice samples of individual or commingled rice cultivars with different To ranges were soaked at different temperatures (25, …


A Simulation Platform For Accurate Prediction Of In-Bin Drying And Storage Of Rough Rice, Houmin Zhong Dec 2015

A Simulation Platform For Accurate Prediction Of In-Bin Drying And Storage Of Rough Rice, Houmin Zhong

Graduate Theses and Dissertations

Natural air (NA), in-bin drying and storage of rough rice generally maintains high grain quality, but the associated slow movement and occasional stagnation of the drying front during the process may result in problems of rice quality reduction, mold growth, and mycotoxin development, especially for rough rice in the top layers of the bin. Using modeling techniques to simulate in-bin rough rice drying in typically-encountered field scenarios may provide a tool for rapidly predicting the grain condition as drying progresses. The objectives for this study were to (1) investigate accurate models for predicting equilibrium moisture content (EMC) of rough rice …


Extraction, Purification And Characterization Of A Pure Peptide From Soybean To Demonstrate Anti-Proliferation Activity On Human Cancer Cells And Test The Ability Of Soy Peptide Fractions In Reducing The Activity Of Angiotensin-I Converting Enzyme, Srinivas Jairam Rayaprolu Dec 2015

Extraction, Purification And Characterization Of A Pure Peptide From Soybean To Demonstrate Anti-Proliferation Activity On Human Cancer Cells And Test The Ability Of Soy Peptide Fractions In Reducing The Activity Of Angiotensin-I Converting Enzyme, Srinivas Jairam Rayaprolu

Graduate Theses and Dissertations

Proteins are of major interest for nutritional value, functionality and sensory characteristics in foods, and biological activity as health-promoting ingredients. A large number of potential biological activities are encrypted within the primary structures of animal and plant proteins. This research has been based on utilizing a legume by-product, soybean meal, to obtain value-added protein products. Forty four Arkansas grown soybean lines were subjected to analysis for protein and amino acid (profile) content to select lines that have high protein attributes. Three soybean lines, R95-1705 (high yielding, non-transgenic and high protein), N98-445A and S03-543CR (bred for high oleic acid - monounsaturated …


Microbial Safety, Shelf Life Stability, Quality Preservation, And Consumer Acceptance Of Vacuum-Infused, Irradiated Chicken Breast Meat Treated With Natural Plant Extracts And Organic Acids, Kenneth Franklin Over Dec 2015

Microbial Safety, Shelf Life Stability, Quality Preservation, And Consumer Acceptance Of Vacuum-Infused, Irradiated Chicken Breast Meat Treated With Natural Plant Extracts And Organic Acids, Kenneth Franklin Over

Graduate Theses and Dissertations

Contamination of poultry products with food borne pathogens is a consistently recurring problem in the United States and the world. Recalls associated with chicken contamination are responsible for considerable economic losses to the poultry industry. Many decontamination steps are currently in place to reduce the likelihood of contaminated products reaching the consumer, but despite these safeguards, contamination events are still uncomfortably frequent. Irradiation is a safe and effective means of ensuring the safety of both ready-to-cook and ready-to-eat poultry but is not utilized in the United States. This is due, in part, to certain irradiation dose-dependent quality characteristic defects associated …


Composition Of Four Selected Arkansas-Grown Non-Gmo Soybean Cultivars And The Development Of High Protein Beverage Using A Selected Soybean Protein Hydrolysate, Quyen Tran Phuong Nguyen Dec 2015

Composition Of Four Selected Arkansas-Grown Non-Gmo Soybean Cultivars And The Development Of High Protein Beverage Using A Selected Soybean Protein Hydrolysate, Quyen Tran Phuong Nguyen

Graduate Theses and Dissertations

Protein beverages made with non-genetically modified (non-GM) ingredients have been in demand due to the increasing consumers’ interest in healthy eating habit. Beverages formulated with non-GM soy protein are rare in the United States market since 93% soybeans planted in U.S (2013) are labeled as GM. This study aimed to develop protein hydrolysate beverages using one cultivar among the four Arkansas grown non-GM soybean cultivars including Osage, R95-1705, R08-4004 and R05-4969. R08-4004 was selected since it had the highest amounts of the three branched amino acids including Phenylalanine (51.4 mg/g), Leucine (74.9 mg/g), and Isoleucine (49.7 mg/g) with lowest amount …


The Future Of Farming In Capable And Small Hands: The Young Farmer’S Movement In Waterloo Region 1907-1924, Morgan Williams Nov 2015

The Future Of Farming In Capable And Small Hands: The Young Farmer’S Movement In Waterloo Region 1907-1924, Morgan Williams

Laurier Undergraduate Journal of the Arts

No abstract provided.


Study On Food Nutrition Of Left-Behind Children By Latent Class Model, Zhang Zhenxia Oct 2015

Study On Food Nutrition Of Left-Behind Children By Latent Class Model, Zhang Zhenxia

Food and Machinery

The latent class model was used to study the special group of children who lacked food nutrition, the left behind children, and the effects were also studied, including the food nutritional status and the individuals genetic and the food and nutrition intake, mothers care, and basic medical and health services. Through research and analysis, two groups of children who were left behind were identified, the food nutrition malnutrition and the food nutrition condition good, and the proportion of malnourished children was higher with 69.4%. In the Two groups of left behind children, the main difference lies in the mother care, …


Design And Research Of Fruit Detection And Grading Machine By Clamping Fruit Stems, Wang Yunxiang, Ma Benxue, Jia Yanting, Yang Jie Oct 2015

Design And Research Of Fruit Detection And Grading Machine By Clamping Fruit Stems, Wang Yunxiang, Ma Benxue, Jia Yanting, Yang Jie

Food and Machinery

No abstract provided.


Design Of Starter-Making Process Control System Using Bidirectional Variable Frequency Vibration Pressing, Li Hong, Wang Wenle, Hua Yong Oct 2015

Design Of Starter-Making Process Control System Using Bidirectional Variable Frequency Vibration Pressing, Li Hong, Wang Wenle, Hua Yong

Food and Machinery

Based on the condition of lower mud extracting ability of starter making, developed a starter making method of rotary bidirectional variable frequency vibration pressing which is designed on Programmable Logic Controller(PLC), and the hardware and software design of PLC field controlling as well as host control system was achieved. The results showed that starter produced by this method had the advantage of density uniformity, good porosity, modest hardness, easy demoulding, smooth surface, exterior condition and full degree surface, and it also has an ideal effect on mud extracting.


Reseach On Quality Changes During Fruit Development Of Chayote, Li Yu, Qin Wen, Du Xiaoqin, Liu Ji Oct 2015

Reseach On Quality Changes During Fruit Development Of Chayote, Li Yu, Qin Wen, Du Xiaoqin, Liu Ji

Food and Machinery

The changes of growth phase and quality indicators during different growth stages of chayote were measured and analyzed. The results indicated that the growth curve of chayote showed single sigmoid pattern and the trends of quality indicators were different with fruit development dynamics. Respiration rate decreased rapidly with fruit development and respiration peak appeared at maturity; the contents of Vc, chlorophyll and hydrolysis amino acids were higher during the young fruit development, then decreased to a low level; while cellulose content and hardness showed the opposite trends. The fruit had fully grown with higher content of amino acids, lower respiratory …


Research Progress In Starch Modification By Heat Moisture Treatment, Liao Luyan, Wu Weiguo Oct 2015

Research Progress In Starch Modification By Heat Moisture Treatment, Liao Luyan, Wu Weiguo

Food and Machinery

The heat moisture treatment refers to the relative lower humidity and temperature in a certain time, and it is a physical modification method of starch properties change. This method costs lower and avoid the pollution by chemical modification, and achieve the purpose of improving starch specific features, which has been showing a good application prospect in modified starch. This paper introduces the effects on the starch structure and properties by heat moisture treatment in detail, and finally expounds the mechanism of the heat moisture treatment starch and the research progress in food applications.


Effect Of Matrix On Glassy Carbohydrate Products Comprising Sweet Orange Oil, Chang Dawei, Liu Shuxing, Liu Ning Oct 2015

Effect Of Matrix On Glassy Carbohydrate Products Comprising Sweet Orange Oil, Chang Dawei, Liu Shuxing, Liu Ning

Food and Machinery

Encapsulation in glassy carbohydrates via extrusion is the low temperature technology and considered to be suitable for encapsulating heat-sensitive materials. The selection of matrix is the important factor. The temperatures of extruder barrel were T1 (65 ℃),T2 (85 ℃),T3 (105 ℃) and T4 (95 ℃). Screw speed was 60 r/min and the feeding speed was 1.2 kg/h. The effects of matrix including maltodextrin, maltodextrin added sucrose and maltodextrin added modified starch on the extrudates was investigated. The results showed that the extruded products were the glassy state. The glass transition temperature (Tg) of the …


Application Of Rice Husk Lignin In The Polyurethane Foam, Shi Gang, Zhu Qinfu, Jiang Shuai, Zhang Xin, Ni Caihua, Li Ying Oct 2015

Application Of Rice Husk Lignin In The Polyurethane Foam, Shi Gang, Zhu Qinfu, Jiang Shuai, Zhang Xin, Ni Caihua, Li Ying

Food and Machinery

Due to the high-cost and non-renewable materials for producing polyurethane foam, the lignin of rice as raw material, partially substituting phenol, was to synthetize lignin polyurethane foam. Firstly, the lignin was extracted from rice through ethyl alcohol. Then, the lignin was modified by epoxy chloropropane to increase the number of hydroxyl groups. The modified lignin was readily soluble in the polyether polyol, and could replace part of polyether polyol to mix with isocyanate and other additives developing resource-efficient polyurethane foam. This study not only provides the feasible solution of producing lignin polyurethane foam, but also makes effective use of agricultural …


Effect Of Xanthan On Gelling Properties Of Myofibrillar Protein-Soy Protein Composite With Various Salt Levels, Lin Yuhui, Li Pengpeng, He Zhiyong, Qin Fang, Wu Shengfang, Chen Jie Oct 2015

Effect Of Xanthan On Gelling Properties Of Myofibrillar Protein-Soy Protein Composite With Various Salt Levels, Lin Yuhui, Li Pengpeng, He Zhiyong, Qin Fang, Wu Shengfang, Chen Jie

Food and Machinery

No abstract provided.


Influence Of Protein And Starch On Commercial Cigarette Sensory Quality By Partial Least Square Regression, Su Jiakun, Luo Juanmin, Xu Da, Zhao Juan, Shen Ning, Yu Jingyang, Xia Shuqin, Cai Jibao, Zhang Xiaoming Oct 2015

Influence Of Protein And Starch On Commercial Cigarette Sensory Quality By Partial Least Square Regression, Su Jiakun, Luo Juanmin, Xu Da, Zhao Juan, Shen Ning, Yu Jingyang, Xia Shuqin, Cai Jibao, Zhang Xiaoming

Food and Machinery

The Partial Least Square Regression (PLSR) analysis was used to study the specific influence of protein and starch contents on sensory quality of 10 different kinds of commercial cigarettes. The results showed that there were significant negative correlation between protein, starch and astringency, throat irritation, nasal irritation, indicating that the lower protein and starch content in tobacco, the lower astringency, throat irritation, nasal irritation and higher sensory quality.


Optimization On Tobacco Powder Extracts By Maillard Reaction, Li Xinqin, Zhang Jing, Tian Zhaofu, Li Zhihua, Zhang Peng, Bai Jiafeng, Yu Jingyang, Long Zhangde, Zhang Xiaoming Oct 2015

Optimization On Tobacco Powder Extracts By Maillard Reaction, Li Xinqin, Zhang Jing, Tian Zhaofu, Li Zhihua, Zhang Peng, Bai Jiafeng, Yu Jingyang, Long Zhangde, Zhang Xiaoming

Food and Machinery

Different kinds of amino acids and saccharides were added to the concentrated tobacco extract for Maillard reaction. Based on the results of single factor tests, the optimized reaction conditions were as follows: ratio of xylose to glycine 2∶1(m∶m), xylose addition 6%, initial pH 6.5, reaction temperature 100 ℃, reaction time 3 h. The evaluation results demonstrated that the toast aroma of Maillard reaction products of xylose and glycine was enhanced significantly, and accompanied by significant reduction of offensive odor and wood. Furthermore, the flavor of product got well improved.The method of SPME was chosen to extract the volatile compounds of …


Effects On Aroma Components Of Weifang Tobacco With Different Drying Methods, Sun Qiang Oct 2015

Effects On Aroma Components Of Weifang Tobacco With Different Drying Methods, Sun Qiang

Food and Machinery

In order to research the influence of aroma components with different drying methods on different parts of tobacco, three parts of Shandong Weifang tobacco was used as the experimental material to study the change of aromatic components in pyrolytic products of cut tobacco at the two methods that sheet tube drying and pneumatic drying. The results showed that, the whole amount of aromatic components increased in both way, sheet tube drying obviously affected the upper and central tobacco, and pneumatic drying was more obvious affectting the underneath tobacco.


Research For Quantitative Classification Of Dry Red Wine In Uniform Color Space, Chen Xiaoyi, Zhang Junxiang, Wang Hong Oct 2015

Research For Quantitative Classification Of Dry Red Wine In Uniform Color Space, Chen Xiaoyi, Zhang Junxiang, Wang Hong

Food and Machinery

The experiment collected 13 samples from the bottle stored red wine which are made in the Eastern Foot of Helan Mountain, 2012 and 2013. Method by ultraviolet spectrophotometer. Quantitative appraisal of color of red wine is established according to the parameters of hue (H*), saturation(C*) and brightness(L*) are analyzed in CIE L*a*b* (recommended by International Commission on Illumination). The hue is divided into 3 levels, the chroma is divided into 5 levels, the lightness is divided into 5 levels. construct the CIE L*a*b* model …


An Edge Detection For Sunflower Seeds Based On Improved Canny Algorithm, Xu Can, Zhang Qiuju Oct 2015

An Edge Detection For Sunflower Seeds Based On Improved Canny Algorithm, Xu Can, Zhang Qiuju

Food and Machinery

Aiming at the edge-detection for a class of objects of irregular shape, such as sunflower seeds, but the traditional Canny algorithm has many shortcomings. This paper proposed three improvements: ① The adaptive high threshold method based on the gradient statistic difference was to overcome the problem that the traditional Canny algorithm might lose part valid edge information when the gray of goals and background in the image had a relatively wide change, which had higher signal-to-noise ratio; ② It proposed an algorithm of extending at the endpoints based on the gradient direction was made up for the defect of getting …


Study On Volatile Flavor Compounds In Different Parts Of Dairy Veal Calf, Yuan Qi, Luo Aiping, He Guangzhong, Zhou Guojun Oct 2015

Study On Volatile Flavor Compounds In Different Parts Of Dairy Veal Calf, Yuan Qi, Luo Aiping, He Guangzhong, Zhou Guojun

Food and Machinery

Determined the difference of brisket, tenderloin, topside from 120 d dairy veal calf. The muscle was sampled at slaughter and subjected to volatile flavor compounds analysis by SPME—GC—MS. The results showed that: the same type of volatile flavor compounds was different in different parts of the veal. Brisket had the least amount of aldehydes, which was only 32.181%. Brisket had the largest amount of hydrocarbons, which is twice as much as tenderloin, and topside. Topside had the lowest level of ketones, which is only 2.928%. Different parts of meat sample had different type and proportion of total volatiles flavor compounds. …


Effects Of Quinolones On Hemolymph Enzymes Of Chinese Mitten Crab, Eriocheir Sinensis, Wang Haichao, Zhang Xingtao, Dong Zeng, Wu Xiao Oct 2015

Effects Of Quinolones On Hemolymph Enzymes Of Chinese Mitten Crab, Eriocheir Sinensis, Wang Haichao, Zhang Xingtao, Dong Zeng, Wu Xiao

Food and Machinery

Norfloxacin, ofloxacin and enorfloxacin, the most common used quinolones in aquaculture, were evaluated for the effects on hemolymph enzymes of Chinese mitten crab, Eriocheir sinensis. The hemolymphs were collected at regular time for detection of phenoloxidase(PO), peroxidase(POD), alkaline phosphatase(ALP), lysozyme(LSZ), superoxide dismutase(SOD), glutamic-oxaloacetic transaminase(AST) and glutamic-pyruvic transaminase(ALT) after Chinese mitten crab were injected intramuscularly with quinolones at concentration of 5 μg/kg. The results showed that activity of PO, POD, ALP, LSZ and SOD increased respectively(P<0.05), and the effect of enorfloxacin experimental group was very obvious(P<0.01), and decreased to the normal levels as the control group at 96 h. The activity of AST and ALT after quinolones injection showed significantly lower during the experimental period(P<0.05). These results indicated that quinolones did not result in inhibition on hemolymph immune enzyme and damage on viscera organs. Moreover, quinolones showed immune enhancement on at concentration of 5 μg/kg.


Optimization On Culture Medium For Laccase Production From Coriolus Hirsutus By Residues Of Traditional Chinese Medicine, Yue Kun, Pan Zhiheng, Sun Yongmin Oct 2015

Optimization On Culture Medium For Laccase Production From Coriolus Hirsutus By Residues Of Traditional Chinese Medicine, Yue Kun, Pan Zhiheng, Sun Yongmin

Food and Machinery

In order to make full use of residue from traditional Chinese medicine, the different kinds of residues of traditional Chinese Medicine for laccase production by Coriolus hirsutus were investigated. It is capable of producing laccase with the three kinds of residues of traditional Chinese Medicine. The result showed that the optimal medium composition was as follows: wheat bran 3%, peptone 02%, residues of Chinese rhubarb 12%, MgSO4 3 g/L,KH2PO4 3 g/L, pH value 5.0. Enzyme activity under the optimal culture conditions was 4 828.2 U/mL, which was 1.4 fold of that of the original medium.


Development Of A Loop-Mediated Isothermal Amplification Assay For Visual Detection Of Staphylococcus Aureus, Song Taoping, Qiu Huali, Wang Shujuan, Peng Xinkai, Yang Lixia Oct 2015

Development Of A Loop-Mediated Isothermal Amplification Assay For Visual Detection Of Staphylococcus Aureus, Song Taoping, Qiu Huali, Wang Shujuan, Peng Xinkai, Yang Lixia

Food and Machinery

To develop a loop-mediated isothermal amplification (LAMP) assay for visual detecting Staphylococcus aureus, 2 sets of specific primers were designed and 6 primers targeting the nuc gene was selected. The method could detect Staphylococcus aureus within 45 min at a constant temperature of 63 ℃. The sensitivity of the method was 1 pg/μL, and had no cross reaction with other pathogens. The result indicated that the LAMP assay was a rapid, specific and simple tool for field diagnosis of Staphylococcus aureus.