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Articles 121 - 150 of 151
Full-Text Articles in Food Processing
Preparation And Anti-Exercise Fatigue Effect Evaluation Of Γ-Aminobutyric Acid-Enriched Milk Fermented With Lactic Acid Bacteria, Dong Liu, Yuning Yang, Fangfei Dou, Yuxing Li, Minghai Ju
Preparation And Anti-Exercise Fatigue Effect Evaluation Of Γ-Aminobutyric Acid-Enriched Milk Fermented With Lactic Acid Bacteria, Dong Liu, Yuning Yang, Fangfei Dou, Yuxing Li, Minghai Ju
Journal of Food Science and Biotechnology
[Objective ] This study aims to prepare γ-aminobutyric acid (GABA )-enriched milk fermented with lactic acid bacteria and evaluate its anti-exercise fatigue effect.[Method ] High GABA-producing lactic acid bacteria were selected and used to ferment milk with a commercial starter culture.A mouse model of exercise fatigue was established by four weeks of exhaustive swimming training.After four weeks,the exhaustive swimming time,serum biochemical indicators,antioxidant indicators,and energy storage substance levels were measured and analyzed.[Result ] Lactobacillus plantarum (L.plantarum ) LP- 5 was selected as the strain for fermentation,and its GABA yield reached (1.340±0.003) g/L.The fermented milk showed the titratable acidity of (46.29±2.01) ºT,the …
Research Progress In Application Of Ultra-High Pressure Sterilization In The Processing Of Prepared Dishes, Haisheng Dong, Peng Zang
Research Progress In Application Of Ultra-High Pressure Sterilization In The Processing Of Prepared Dishes, Haisheng Dong, Peng Zang
Journal of Food Science and Biotechnology
As a non-thermal sterilization technology,ultra-high pressure sterilization can protect the thermosensitive nutrients and maintain the texture of food,demonstrating unique technical advantages in the processing of prepared dishes.By a review of domestic and foreign literature and performance of relevant equipment,this paper introduced the principle,equipment,and parameters of ultra-high pressure sterilization.Considering the parameters of ultra-high pressure sterilization and the effects of food ingredients on the pressure tolerance of different microbial species,the factors affecting the effect of ultra-high pressure sterilization were analyzed.In addition,the effects of ultra-high pressure sterilization on the physical and chemical properties,sensory quality,enzyme activity,microorganisms count and bioactive substances of prepared dishes were …
Research Progress In Biological Activities , Mechanisms Of Action , And Applications Of Antimicrobial Peptides, Yuxin Jiao, Ruolan Shan, Xufeng Wang, Maolong Chen, Li Wen, Yunhui Cheng, Hao Wu
Research Progress In Biological Activities , Mechanisms Of Action , And Applications Of Antimicrobial Peptides, Yuxin Jiao, Ruolan Shan, Xufeng Wang, Maolong Chen, Li Wen, Yunhui Cheng, Hao Wu
Journal of Food Science and Biotechnology
The widespread abuse of traditional antibiotics has led to the emergence and development of antimicrobial peptides due to the resultant bacterial resistance.Antimicrobial peptides demonstrate high efficacy in combating infectious pathogens and possess unique antimicrobial mechanisms and biocompatibility,effectively reducing the bacterial resistance rate.The high structural adaptability endows antimicrobial peptides with multiple biological activities,enabling targeted action against diverse types of pathogens and abnormal cells.Accordingly,antimicrobial peptides exhibit a promising development prospect.Enhanced stability contributes to prolonging the in vivo half-life and improving the bioavailability of antimicrobial peptides,which holds crucial importance for improving the clinical translation value of antimicrobial peptides.The author summarizes the research progress …
Mechanisms Of The Quality Changes Of Polygonati Rhizoma Processed By Steaming-Drying Cycles, Zicong Yu, Liuping Fan
Mechanisms Of The Quality Changes Of Polygonati Rhizoma Processed By Steaming-Drying Cycles, Zicong Yu, Liuping Fan
Journal of Food Science and Biotechnology
[Objective ] This study aims to investigate the effects of steaming-drying cycles on the quality changes of Polygonati rhizoma and optimize the processing technology to enhance its application value.[Method ] Slices of Polygonati rhizoma processed by 0~5 steaming-drying cycles were prepared.The drying rate and drying kinetics during the drying stage after each cycle were analyzed,and a drying model was fitted.Changes in color difference,appearance,hardness,and mass fractions of polysaccharides,reducing sugars,polyphenols,flavonoids,saponins,and calcium oxalate raphides were measured.[Result ] The number of steaming-drying cycles significantly affected the quality of Polygonati rhizoma.The drying rate decreased as the drying time increased,conforming to the Modified Page model,while the …
Determination Of Microorganisms And Analysis Of Factors Influencing Osmophilic Yeasts In Apis Cerana Honey, Mei Song, Hongxia Li, Zhaolong Liu, Dong Qiao, Na Li, Lanzhen Chen
Determination Of Microorganisms And Analysis Of Factors Influencing Osmophilic Yeasts In Apis Cerana Honey, Mei Song, Hongxia Li, Zhaolong Liu, Dong Qiao, Na Li, Lanzhen Chen
Journal of Food Science and Biotechnology
[Objective ] This study determined the mold count,total plate count,and osmophilic yeast count in Apis cerana honey and explored the correlations of osmophilic yeasts with quality parameters and storage conditions of the honey.[Method ] A total of 105 Apis cerana honey samples were used for the determination of mold count,total plate count,and osmophilic yeast count,as well as odor,moisture,phenolic acids,and carbohydrates.The correlations between quality indexes and osmophilic yeasts and the effects of different storage conditions on osmophilic yeast count were explored.[Result ] Among the 105 samples,1,1,and 67 samples showed the mold count,total plate count,and osmophilic yeast count exceeding the national standards.All …
Photosensitizer-Loaded Glycogen For Photodynamic Therapy Of Tumor, Keke Ma, Jiurui Fang, Huijie Zhang, Jinghua Chen
Photosensitizer-Loaded Glycogen For Photodynamic Therapy Of Tumor, Keke Ma, Jiurui Fang, Huijie Zhang, Jinghua Chen
Journal of Food Science and Biotechnology
[Objective ] The aim of this study is to synthesize aminated glycogen (NG) for loading of the photosensitizer TTVBA and construct a nano drug delivery system (NGT NPs ) for photodynamic therapy (PDT ) of tumors.[Method ] Positively charged NG was synthesized through modification of glycogen with diethylenetriamine (DETA ).Then,TTVBA was encapsulated by NG via electrostatic interaction to prepare NGT NPs.The prepared NGT NPs were characterized by infrared spectroscopy,dynamic light scattering (DLS ),and transmission electron microscopy (TEM ).The hemolysis assay was performed to evaluate the biocompatibility of NGT NPs.The reactive oxygen species (ROS ) generation capability of cells treated with …
Prokaryotic Expression , Purification , And Polyclonal Antibody Preparation Of Chlamydomonas Reinhardtii Channelrhodopsin 1, Jiurong Wang, Bin Xue, Zhenchuan Fan
Prokaryotic Expression , Purification , And Polyclonal Antibody Preparation Of Chlamydomonas Reinhardtii Channelrhodopsin 1, Jiurong Wang, Bin Xue, Zhenchuan Fan
Journal of Food Science and Biotechnology
[Objective ] This study aims to prepare a highly specific antibody against channelrhodopsin- 1 (ChR 1) from Chlamydomonas reinhardtii (C.reinhardtii ) and investigate its regulatory mechanisms in cellular phototaxis and negative phototaxis.[Method ] The recombinant expression vector pET- 28a(+)-chr1 was constructed and transformed into Escherichia coli (E.coli ) BL21 (DE3) competent cells.The expression of the fusion protein 6×His∷ChR 1 was induced with IPTG,and the obtained protein was purified by nickel-affinity chromatography.The purified protein was used to immunize New Zealand white rabbits for the production of ChR 1 antiserum.ChR 1-specific IgG antibodies were enriched from the antiserum through protein A purification.The …
Engineering Escherichia Coli Tolerance To Improve Succinic Acid Production, Jingyu Pan, Yongsheng Tang, Jia Liu, Cong Gao, Xiaomin Li, Wei Song, Wanqing Wei, Guipeng Hu, Jing Wu, Liming Liu, Xiulai Chen
Engineering Escherichia Coli Tolerance To Improve Succinic Acid Production, Jingyu Pan, Yongsheng Tang, Jia Liu, Cong Gao, Xiaomin Li, Wei Song, Wanqing Wei, Guipeng Hu, Jing Wu, Liming Liu, Xiulai Chen
Journal of Food Science and Biotechnology
[Objective ] This study aims to enhance the high osmotic pressure tolerance of Escherichia coli (E.coli ) for the efficient production of succinic acid.[Method ] Adaptive evolution,transcriptomic analysis,reverse metabolic engineering,and fermentation optimization were employed for improving E.coli tolerance to high osmotic pressure.[Result ] By analyzing the key factors influencing succinic acid production by fermentation,osmotic pressure was identified as the critical factor reducing succinic acid production efficiency.A mutant strain,E.coli FN-6,with tolerance to high osmotic pressure was obtained through adaptive evolution.The key gene cusC responsible for osmotic pressure tolerance was identified by transcriptomic analysis.After optimization of the expression level of cusC,the engineered …
Study On The Antioxidant Effects Of Natural Antioxidants And Their Derivatives On Anchovy (Engraulis Japonicus ) Oil, Xuyang Ding, Fujun Liu, Fawen Yin, Dayong Zhou
Study On The Antioxidant Effects Of Natural Antioxidants And Their Derivatives On Anchovy (Engraulis Japonicus ) Oil, Xuyang Ding, Fujun Liu, Fawen Yin, Dayong Zhou
Journal of Food Science and Biotechnology
[Objective ] The aim of this study is to evaluate and improve the antioxidant effects of natural antioxidants and their derivatives on anchovy (Engraulis japonicus ) oil.[Method ] The 60 ℃ accelerated oxidation model was used to evaluate the antioxidant effects of phytic acid,tea polyphenols (TP),rosemary extract,ascorbyl palmitate,vitamin E,tea polyphenol palmitate (TPP),and bamboo leaf extract on anchovy oil based on the peroxide value (POV ),thiobarbituric acid reactive substances (TBARS ) value,relative intensity of free radicals,and fatty acid composition.[Result ] TPP (single antioxidant ) and TPP+TP (composite antioxidant ) demonstrated the strongest antioxidant effects on anchovy oil.[Conclusion ] Although the antioxidant …
Establishment Of Surface-Enhanced Raman Spectroscopy For Detection Of Escherichia Coli O157:H7, Panxue Wang, Cen Li, Xiang Li, Ying Liu, Guoliang Li
Establishment Of Surface-Enhanced Raman Spectroscopy For Detection Of Escherichia Coli O157:H7, Panxue Wang, Cen Li, Xiang Li, Ying Liu, Guoliang Li
Journal of Food Science and Biotechnology
[Objective ] This study aims to improve the detection specificity and sensitivity for Escherichia coli (E.coli ) O157:H7.[Method ] A high ly specific and sensitive method for the detection of E.coli O157:H7 was established based on magnetic separation and signal amplification strategies combined with surface-enhanced Raman spectroscopy (SERS ).Aptamers were used as recognition elements and combined with magnetic beads and polystyrene (PS) microspheres to construct a separation probe (MB@Apt ) and a signal amplification Raman tag (4-MBN@PS@Apt ),respectively.[Result ] In the presence of E.coli O157:H7,MB@Apt and 4-MBN@PS@Apt formed a “sandwich ” structured composite (MB@Apt/ E.coli O157:H7/4-MBN@PS@Apt ),which could be rapidly …
Rapid Determination Of Vitamin B 1 In Infant Formula Milk Powder By Fluorescent Microsphere Immunochromatographic Assay, Qianqian Lu, Ting Yang, Yongwei Feng, Jialin Hu, Hua Kuang, Chuanlai Xu, Lingling Guo
Rapid Determination Of Vitamin B 1 In Infant Formula Milk Powder By Fluorescent Microsphere Immunochromatographic Assay, Qianqian Lu, Ting Yang, Yongwei Feng, Jialin Hu, Hua Kuang, Chuanlai Xu, Lingling Guo
Journal of Food Science and Biotechnology
[Objective ] A fluorescent immunochromatographic assay was established based on vitamin B 1 monoclonal antibody to measure the vitamin B 1 in infant formula milk powder.[Method ] The performance of vitamin B 1 monoclonal antibody was evaluated.On this basis,a highly sensitive fluorescent immunochromatographic test strip was developed.[Result ] The vitamin B 1 monoclonal antibody showed high sensitivity,affinity,and specificity.The fluorescent immunochromatographic test strip showed a visual limit of detection (vLOD ) of 10 μg/kg,a cut-off value of 200 μg/kg,and a calculated limit of detection (cLOD ) of 2.056 μg/kg.In addition,the recovery rate of this method was 97.3%~115.4%.The test results of the …
Colloidal Carbon-Labelled Immunochromatographic Assay For Rapid Detection Of Marbofloxacin Residues In Animal-Derived Foods, Xiujin Chen, Biao An, Jian Tao, Xinhao Yin, Yao Wang, Huawei Niu, Zhaozhou Li, Min Zhang
Colloidal Carbon-Labelled Immunochromatographic Assay For Rapid Detection Of Marbofloxacin Residues In Animal-Derived Foods, Xiujin Chen, Biao An, Jian Tao, Xinhao Yin, Yao Wang, Huawei Niu, Zhaozhou Li, Min Zhang
Journal of Food Science and Biotechnology
[Objective ] A colloidal carbon-labelled immunochromatographic assay with competitive inhibition was developed for the detection of marbofloxacin (MBF ) residues in milk powder and chicken.[Method ] The anti-MBF polyclonal antibody prepared in the author 's laboratory was employed to produce the colloidal carbon-labelled immunoprobe.The effects of pH of reaction system,resuspension solution,treatment solution of sample pad,standard dilution buffer,mass concentration of colloidal carbon,mass concentration of primary antibody,mass concentration of coating antigen,and mass concentration of secondary antibody on the MBF colloidal carbon-labelled immunochromatographic assay were investigated.The sensitivity,specificity,stability,and accuracy of the method were determined.[Result ] Under optimal conditions,the cut-off value of the method was …
Construction Of Staphylococcus Aureus Detection Method Based On, Silver Nanoclusters Dna, Xiaotong Lu, Jinzhao Li, Xinjun Du
Construction Of Staphylococcus Aureus Detection Method Based On, Silver Nanoclusters Dna, Xiaotong Lu, Jinzhao Li, Xinjun Du
Journal of Food Science and Biotechnology
[Objective ] A simple and sensitive detection method for Staphylococcus aureus is established for the efficient detection of this pathogen in food.[Method ] Targeting Staphylococcus aureus,a simple and sensitive fluorescent detection method for Staphylococcus aureus,EXPAR-P-AgNCs,was developed based on exponential amplification reaction (EXPAR ) combined with DNA silver nanoclusters (P-AgNCs ).A recognition probe was designed to initiate EXPAR in the presence of Staphylococcus aureus,generating a large amount of target DNA(T).The binding of T to hairpin P displaced the G-rich strand from P-AgNCs,resulting in a decrease in the relative fluorescence intensity of P-AgNCs and enabling the detection of Staphylococc us aureus.[Result ] …
Physicochemical Indicators , Microbial Diversity , And Volatile Substances In The Qupi And Quxin Of High-Temperature Daqu, Dan Xie, Cheng Wu, Wenyu Zhao, Jianfeng Hu, Pingyan Cheng, Yuanlin Bi, Junlin Yang, Xiaolong You, Diqiang Wang
Physicochemical Indicators , Microbial Diversity , And Volatile Substances In The Qupi And Quxin Of High-Temperature Daqu, Dan Xie, Cheng Wu, Wenyu Zhao, Jianfeng Hu, Pingyan Cheng, Yuanlin Bi, Junlin Yang, Xiaolong You, Diqiang Wang
Journal of Food Science and Biotechnology
[Objective ] This study aims to explore the physicochemical indicators,microbial diversity,and volatile substances in the Qupi (surface ) and Quxin (core) of high-temperature Daqu.[Method ] High-throughput sequencing and headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS ) were employed to compare the microbial diversity,volatile substances,and physicochemical indicators between Qupi and Quxin of three types of Daqu.[Result ] The moisture mass fraction in Qupi of the three types of Daqu was lower than that in Quxin (P<0.05),and the moisture mass fraction was the lowest in the Qupi of white Daqu (WP) and the highest in the Quxin of yellow Daqu (YX).The acidity of Qupi and Quxin were in the order of Quxin of black Daqu (BX) > YX > Qupi of black Daqu (BP) > Quxin of white Daqu (WX) > Qupi of yellow Daqu (YP) > WP.High-throughput sequencing results showed that Firmicutes and …
Heterologous Expression And Application Of A Thermophilic Lytic Polysaccharide Monooxygenase, Le Xie, Longyun Peng, Yun Hu, Hongyan Ren, Haiyan Sun, Fubao Sun
Heterologous Expression And Application Of A Thermophilic Lytic Polysaccharide Monooxygenase, Le Xie, Longyun Peng, Yun Hu, Hongyan Ren, Haiyan Sun, Fubao Sun
Journal of Food Science and Biotechnology
[Objective ] This study aims to investigate the application of a novel thermophilic lytic polysaccharide monooxygenase (LPMO ) in the efficient cellulase-mediated hydrolysis of lignocellulosic substrates.[Method ] The thermophilic enzyme mgLPMO 10,derived from a compost metagenome,was heterologously expressed in Escherichia coli (E.coli ).Its expression level was improved by modifying the signal peptide of the expression vector and induction conditions (temperature,IPTG concentration,and induction time ).The enzyme was then characterized in terms of the optimal temperature,optimal pH,thermal stability,pH stability,and the effects of metal ions on its enzyme activity.Subsequently,the synergistic hydrolysis efficiency of mgLPMO 10 with two thermophilic endoglucanases (TnCelB and DtCelB ) …
Preparation , Performance , And Application Of A High-Performance Affinity Chromatography Medium Based On Staphylococcus Aureus Protein A, Zihao Guo, Chaoqing Yan, Haifeng Xia
Preparation , Performance , And Application Of A High-Performance Affinity Chromatography Medium Based On Staphylococcus Aureus Protein A, Zihao Guo, Chaoqing Yan, Haifeng Xia
Journal of Food Science and Biotechnology
[Objective ] Staphylococcus aureus protein A (SPA ) is widely used for the purification of monoclonal antibodies due to its high specificity for immunoglobulin G (IgG).However,the high costs of commercial media have limited the further development of SPA.To address this issue,this paper aims to prepare a high-performance affinity chromatography medium based on SPA as the substitute of introduced affinity chromatography media.[Method ] SPA expressed by the recombinant strain was purified via Ni2+ affinity chromatography and then coupled to the Rigose HF matrix to prepare an self-prepared affinity chromatography medium.[Result ] The self-prepared medium had a static saturated adsorption capacity of …
Phrg-Rapg-Degu Quorum Sensing Regulates Menaquinone- 7 Production In Bacillus Subtilis, Junbao Huang, Xilin Huang, Yu Chen, Xuli Gao, Yani Luo, Wei Tao, Yongyuan Liu, Mingyu Guo, Jing Wu, Yan Liu
Phrg-Rapg-Degu Quorum Sensing Regulates Menaquinone- 7 Production In Bacillus Subtilis, Junbao Huang, Xilin Huang, Yu Chen, Xuli Gao, Yani Luo, Wei Tao, Yongyuan Liu, Mingyu Guo, Jing Wu, Yan Liu
Journal of Food Science and Biotechnology
[Objective ] The effects of the transcription regulation system on menaquinone- 7 (MK- 7) production in Bacillus subtilis (B.subtilis ) 168 were studied by excavating transcription regulators.[Method ] With the signal molecule PhrG pentapeptide,RapG,and the transcription regulator DegU,a PhrG-RapG-DegU regulatory system was successfully constructed in B.subtilis 168 to fine-tune the expression of key genes,so that the expression of key genes in the MK- 7 synthesis pathway can respond to the changes in cell density.[Result ] The production of MK- 7 in strain BS 06 reached 102.47 mg/L through this system.[Conclusion ] Fine tuning of quorum sensing can effectively improve the …
Effects Of Edible Fungal Polysaccharides On The Proliferation And Metabolism Of Lactobacillus Rhamnosus Gg, Shengyao Wu, Zikun Ye, Weiyi Xie, Yi Wang, Haotian Ouyang, Chengcheng Zhang, Shenyan Ma
Effects Of Edible Fungal Polysaccharides On The Proliferation And Metabolism Of Lactobacillus Rhamnosus Gg, Shengyao Wu, Zikun Ye, Weiyi Xie, Yi Wang, Haotian Ouyang, Chengcheng Zhang, Shenyan Ma
Journal of Food Science and Biotechnology
[Objective ] The aim of this study is to explore the effects of edible fungal polysaccharides on the proliferation and metabolism of Lactobacillus rhamnosus GG (LGG ).[Method ] Flarnmulina velutipes polysaccharides (FVP ),Tremella fueiformls polysaccharides (TFP),Auricularia auricula polysaccharides (AAP ),lentinan (LNT ),Hericium erinaceus polysaccharides (HEP ),and Poria cocos polysaccharides (PCP ) were selected as carbon sources for the culture of LGG.The viable bacterial count and the concentration of short-chain fatty acids (SCFAs ) were determined.Two polysaccharides were selected from the six polysaccharides and added in different mass ratios to the LGG-fermented milk,and then the pH and textural properties of the …
Nutritional Components And Immunoregulatory Mechanisms Of Lactoferrin Formula, Mengying Zhou, Liujie Zheng, Ruiqing Yao, Hongyu Wu, Gangliang Hu, Zhengquan Huo, Yinhua Ni
Nutritional Components And Immunoregulatory Mechanisms Of Lactoferrin Formula, Mengying Zhou, Liujie Zheng, Ruiqing Yao, Hongyu Wu, Gangliang Hu, Zhengquan Huo, Yinhua Ni
Journal of Food Science and Biotechnology
[Objective ] This study aims to investigate the immunoregulatory mechanisms of lactoferrin formula and its four nutritional components (α-lactalbumin,N-acetylneuraminic acid,yeast β-glucan,and lactoferrin ) in RAW 264.7 macrophages.[Method ] M1 and M 2 macrophage polarization were induced by lipopolysaccharides (LPS) and interleukin (IL)-4,respectively.Quantitative real-time PCR,flow cytometry,and Western blot were employed to analyze macrophage polarization and related signaling pathways.[Result ] Lactoferrin formula enhanced the phagocytic activity of macrophages and dose-dependently inhibited M 1 polarization while promoting M 2 polarization within the range of 10~50 µg/mL.A combination of four nutritional components exhibited similar effects,with greater effects than the three-component combination.Additionally,the lactoferrin formula upregulated …
Isolation And In Vitro Inhibition Of Mycotoxin-Metabolizing Strains From Domestic Barley, Ran Qiu, Junhui Zhong, Xiaomin Chang, Lin Xiao, Yanlong Bai, Tengfei Yu
Isolation And In Vitro Inhibition Of Mycotoxin-Metabolizing Strains From Domestic Barley, Ran Qiu, Junhui Zhong, Xiaomin Chang, Lin Xiao, Yanlong Bai, Tengfei Yu
Journal of Food Science and Biotechnology
[Objective ] This study aims to assess the effects of Fusarium on the malting process and malt quality of barley,screen out the microorganism with inhibitory activity against Fusarium,and explore the potential of this microorganism for degrading deoxynivalenol (DON ).[Method ] The mycotoxin-producing strain was isolated from domestic Dan ’er barley contaminated with pathogenic fungi,and the interaction between the pathogen and the host was studied.The germination rate,water sensitivity,and DON mass fraction were compared between the barley infected for 5 d and standard barley.The DON mass fraction,activities of protease,β-glucanase,and amylase,and physicochemical indicators (free amino acids,soluble nitrogen,and β-glucan mass concentration ) of malt …
Research Progress In Effects Of Bile Acids And Their Receptors On Intestinal Function, Lu Jin, Jiansheng Jin, Yongqiang Gu, Lulu Zhou, Feifei Han
Research Progress In Effects Of Bile Acids And Their Receptors On Intestinal Function, Lu Jin, Jiansheng Jin, Yongqiang Gu, Lulu Zhou, Feifei Han
Journal of Food Science and Biotechnology
Bile acids are the main end products of cholesterol metabolism in the body,playing an important regulatory role in the digestion and metabolism of nutrients,especially lipids.The intestine is the main organ for digestion and absorption,as well as an important site for bile acids to achieve liver-intestinal circulation.Its function is closely related to the interaction between bile acids as signaling molecules and their receptors.Focusing on the metabolism and regulatory mechanism of bile acids,the author reviews the roles and underlying mechanisms of bile acids and their receptors in regulating intestinal barriers,immune function,and gut microbiota.The aim is to provide reference for related research on …
In Vitro Probiotic Effects Of Dextran With A Small Molecular Weight, Dingkun Zhang, Lei Wang, Ziling Yi, Beibei Wei, Deming Rao, Zhiyong Guo, Sheng Chen, Jing Wu
In Vitro Probiotic Effects Of Dextran With A Small Molecular Weight, Dingkun Zhang, Lei Wang, Ziling Yi, Beibei Wei, Deming Rao, Zhiyong Guo, Sheng Chen, Jing Wu
Journal of Food Science and Biotechnology
[Objective ] The aim of this study is to explore the effects of dextran with a small molecular weight (SDex ) on the viability of intestinal microorganisms.[Method ] The probiotic effects of laboratory-designed SDex containing only α-1,6 glycosidic bonds was evaluated by in vitro fermentation with nine typical intestinal strains,including strains from the genera Bifidobacterium and Lactobacillus,as well as species such as Escherichia coli and Enterococcus faecalis.The microbial proliferation and organic acid production during fermentation were compared among SDex,commercial dextran 20 (Dex 20),and isomalto-oligosaccharides (IMOs ).[Result ] SDex and IMOs significantly promoted the proliferation of probiotics such as Bifidobacterium and …
Application Of Crispr/Cas System-Based Biosensors In Detection Of Foodborne Pathogens, Miao Zou, Jingyi Zeng, Dandan Zeng, Yuhan Fu, Xu Zhang, Bo Zhao, Tianlu Mo
Application Of Crispr/Cas System-Based Biosensors In Detection Of Foodborne Pathogens, Miao Zou, Jingyi Zeng, Dandan Zeng, Yuhan Fu, Xu Zhang, Bo Zhao, Tianlu Mo
Journal of Food Science and Biotechnology
The CRISPR/Cas system can recognize foreign DNA,cut the target,and block or silence the expression of the foreign gene.Due to its precise targeting function,the CRISPR/Cas system has been developed as a highly efficient gene editing tool.With high specificity and operability,the CRISPR/Cas system has been combined with biosensors to improve the detection specificity and efficiency of the sensors.This review introduces the characteristics and applications of CRISPR/Cas system,analyzes the latest progress in the application of CRISPR/Cas system-based biosensors in the detection of foodborne pathogens,and summarizes and prospects the application of such biosensors.
Antibiotic Resistance And Transmission Characteristics Of Enterococcus Faecalis From The Pork Production Chain In Hangzhou, Min Qian, Wei Zhou, Zhijin Zhou, Enbao Zhang, Jianzhong Han, Daofeng Qu
Antibiotic Resistance And Transmission Characteristics Of Enterococcus Faecalis From The Pork Production Chain In Hangzhou, Min Qian, Wei Zhou, Zhijin Zhou, Enbao Zhang, Jianzhong Han, Daofeng Qu
Journal of Food Science and Biotechnology
[Objective ] This study aims to evaluate the antibiotic resistance and transmission risk of Enterococcus faecalis (E.faecalis ) in the pork production chain.[Method ] A total of 300 samples were collected from the surface of live pigs,carcasses,and the environment in 2 pig farms,1 slaughterhouse,and 2 markets in Hangzhou,Zhejiang.The strain identification,antibiotic resistance phenotypes,resistance genotypes,and virulence genotypes were performed by 16S rDNA sequences,broth microdilution method (MIC ),and PCR.In addition,multilocus sequencing typing (MLST ) and conjugational transfer tests were conducted.[Result ] The 125 isolates of E.faecalis showed high resistance to antibiotics such as tiamulin,tetracycline,and doxycycline,and the isolates from pig farms showed higher antibiotic …
Evaluation Of The Clinical Alleviation Effect Of Bacillus Coagulans Ccfm 1041 On Functional Constipation, Huahao Cai, Linlin Wang, Qingwei Yao, Xudan Shen, Xingxing Chen, Shumao Cui
Evaluation Of The Clinical Alleviation Effect Of Bacillus Coagulans Ccfm 1041 On Functional Constipation, Huahao Cai, Linlin Wang, Qingwei Yao, Xudan Shen, Xingxing Chen, Shumao Cui
Journal of Food Science and Biotechnology
[Objective ] Functional constipation is a chronic gastrointestinal disorder caused by multiple factors,with a relatively high and increasing prevalence in China.Previous studies have found that Bacillus coagulans (B.coagulans )CCFM 1041 can alleviate symptoms of functional constipation in mice.[Method ] A double-blind,randomized controlled clinical trial was conducted to evaluate the clinical efficacy of CCFM 1041 in relieving functional constipation.A total of 73 participants were observed for changes in constipation-related indicators,serum levels of gastrointestinal regulatory neurotransmitters,and gut microbiota before and after the intervention.[Result ] After taking CCFM 1041,84.2% of participants experienced increased bowel movement frequency,and 94.7% of participants showed improved stool consistency.Compared …
Changes In Volatile Aldehydes Of Soymilk After Acid-Precipitation And Its Influencing Factors, Mapeizhan Lou, Caimeng Zhang, Xiangzhen Kong, Xingfei Li, Yeming Chen, Xiaoxiao Feng, Yufei Hua
Changes In Volatile Aldehydes Of Soymilk After Acid-Precipitation And Its Influencing Factors, Mapeizhan Lou, Caimeng Zhang, Xiangzhen Kong, Xingfei Li, Yeming Chen, Xiaoxiao Feng, Yufei Hua
Journal of Food Science and Biotechnology
[Objective ] Acid precipitation,a common method in plant protein processing,can efficiently achieve protein enrichment and removal of non-protein components (including off-flavor substances ) on a large scale.This study aims to explore the causes for the abnormal increases in aldehydes of soymilk after acid precipitation.[Method ] Changes in volatile compounds of soymilk before and after acid precipitation were analyzed by gas chromatography-mass spectrometry (GS-MS ).Soymilk components were fractionated by centrifugation,and the contributions of different fractions to the increases in aldehydes were investigated.The changes in the molar concentrations of lipid hydroperoxides before and after acid precipitation were determined,and a molar ratio between …
Identification Of Honey Authenticity By Terahertz Spectroscopy, Luelue Huang, Zhiqi Wang, Miaoling Liu, Bin Li
Identification Of Honey Authenticity By Terahertz Spectroscopy, Luelue Huang, Zhiqi Wang, Miaoling Liu, Bin Li
Journal of Food Science and Biotechnology
[Objective ] This study aims to identify honey authenticity by terahertz (THz) spectroscopy.[Method ] Different honey samples were analyzed by stable isotope ratio mass spectrometry (IRMS ),and the results served as a reference for terahertz spectroscopy.The sample requirements and analytical conditions of terahertz spectroscopy were verified by comparing the results of these two methods.[Result ] Liquid honey samples exhibited no discernible absorption peaks.However,significant differences in refractive index were observed among samples.The presence of water in liquid honey,particularly at high levels,affected the refractive index,potentially leading to misjudgment.After removal of water from liquid honey via freeze-drying,terahertz attenuated total reflection was employed for …
Effects Of Lactobacillus Plantarum Fermentation On Flavonoids And Organic Acids In Mango Juice, Ying Yang, Dongdong Yang, Li Li, Xuemei He, Dongqing Ye
Effects Of Lactobacillus Plantarum Fermentation On Flavonoids And Organic Acids In Mango Juice, Ying Yang, Dongdong Yang, Li Li, Xuemei He, Dongqing Ye
Journal of Food Science and Biotechnology
[Objective ] This study aimed to investigate the effects of Lactobacillus plantarum (L.plantarum ) fermentation on the metabolism of flavonoids and organic acids in mango juice.[Method ] Six strains of L.plantarum were selected for fermentation of the juice samples from two mango varieties ‘Tainong' and ‘Guiqi'.The mass concentrations of organic acids and flavonoids in the juice samples were determined by ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS ).Orthogonal partial least squares discriminant analysis was employed to compare the metabolites in the mango juice samples fermented with different strains.[Result ] L.plantarum fermentation significantly enhanced the total antioxidant capacity (T-AOC ) …
Effects Of Palmitic Acid-Rich Diet On Tumor Growth And Epithelial-Mesenchymal Transition In The Mouse Model Of Prostate Cancer, Jiayao Chen, Fengjiao Huang, Bingqian Sun, Yongquan Chen, Xiaoying Wang
Effects Of Palmitic Acid-Rich Diet On Tumor Growth And Epithelial-Mesenchymal Transition In The Mouse Model Of Prostate Cancer, Jiayao Chen, Fengjiao Huang, Bingqian Sun, Yongquan Chen, Xiaoying Wang
Journal of Food Science and Biotechnology
[Objective ] This study aims to explore the effect of palmitic acid (PA)-rich diet on prostate cancer (PCa) in mice.[Method ] The TCGA database and immunohistochemistry (IHC) were used to analyze the differential expression of fatty acid synthase (FASN ) between the PCa tissue and adjacent normal tissue.In addition,twelve nude mice were subcutaneously implanted with LNCaP cells and assigned into two groups.One group was fed a PA-rich diet,while the other group was fed a normal control (NC) diet.Twelve mice overexpressing the C-myc gene (C-mycT) were assigned into two groups and fed the same diets as the nude mice.The growth of …
Metagenomic Analysis Of Microbial Community Structure And Functional Genes In Horse Milk From Northern Xinjiang, Gaoshaer Kayierhali, Zhiang Fu, Danhua Ouyang, Haini Bayinda, Bakhetgul Manatbay
Metagenomic Analysis Of Microbial Community Structure And Functional Genes In Horse Milk From Northern Xinjiang, Gaoshaer Kayierhali, Zhiang Fu, Danhua Ouyang, Haini Bayinda, Bakhetgul Manatbay
Journal of Food Science and Biotechnology
[Objective ] Horse milk has a high nutritional value and a unique flavor,which are closely related to its complex microbial composition,and different geographical locations and climates will affect the microbial community structure of horse milk.This study aims to explore the effects of different geographical locations on the microbial community structure in horse milk.[Method ] Metagenomic technology was employed to analyze the microbial diversity of 14 batches of horse milk samples from 3 different regions in northern Xinjiang,and the functional genes were excavated.[Result ] A total of 26 species of microorganisms were identified,belonging to 9 phyla,14 classes,25 orders,28 families,and 45 genera.The …