Food Microbiology Commons^™

Phages Of Non-Diary Lactococci: Isolation And Characterization Of Phi L47, A Phage Infecting The Grass Isolate Lactococcus Lactis Ssp Cremoris Dpc6860, Daniel Cavanagh, Caitríona M. Guinane, Horst Neve, Aidan Coffey, R. Paul Ross, Gerald F. Fitzgerald, Olivia Mcauliffe

Department of Biological Sciences Publications

Lactococci isolated from non-dairy sources have been found to possess enhanced metabolic activity when compared to dairy strains. These capabilities may be harnessed through the use of these strains as starter or adjunct cultures to produce more diverse flavor profiles in cheese and other dairy products. To understand the interactions between these organisms and the phages that infect them, a number of phages were isolated against lactococcal strains of non-dairy origin. One such phage, ΦL47, was isolated from a sewage sample using the grass isolate L. lactis ssp. cremoris DPC6860 as a host. Visualization of phage virions by transmission electron …

Physical And Biochemical Factors Affecting Breast Fillet Tenderness In Broilers Reared For Divergent Market Demands, Valerie Belle Brewer

Graduate Theses and Dissertations

Market trends have dictated that broilers be reared for divergent market demands for decades. Also, broiler genetic strains have been adapted to meet market demands which include: genetic selection for improved breast meat, the practice of growing birds to older ages and greater market weights to meet demands, and decreasing postmortem (PM) aging time to improve processing efficiency and fillet yield. These production practices can also affect product quality, specifically boneless breast fillet tenderness and water-holding capacity. To address these factors, a series of experiments were conducted to determine the effect of strain and slaughter age on meat quality parameters, …

Food, Parasites, And Epidemiological Transitions: A Broad Perspective, Karl Reinhard, Luis Fernando Ferreira, Françoise Bouchet, L. Sianto, J. M.F. Dutra, A. Iniguez, Daniela Leles, M. Le Bailley, Martín Horacio Fugassa, Elisa Pucu, Adauto Araújo

Karl Reinhard Publications

Pathoecology provides unique frameworks for understanding disease transmission in ancient populations. Analyses of Old and New World archaeological samples contribute empirically to our understanding of parasite infections. Combining archaeological and anthropological data, we gain insights about health, disease, and the way ancient people lived and interacted with each other and with their environments. Here we present Old and New World parasite evidence, emphasizing how such information reflects the different ways ancient populations exploited diverse environments and became infected with zoonotic parasites. It is clear that the most common intestinal helminthes (worm endoparasites) were already infecting ancient inhabitants of the New …

Development Of Rapid Detection Methods And Novel Control Measures For Salmonella In Poultry, Si Hong Park

Graduate Theses and Dissertations

This dissertation consists of three research parts: 1) development of rapid detection methods for foodborne pathogens; 2) immune response of chicken cells against Salmonella and bacteriophage P22; 3) evaluation of novel control measures for poultry productions. In order to develop rapid and accurate detection methods for foodborne pathogens, two types of PCR assays were utilized. Three foodborne pathogens included Campylobacter, Escherichia coli and Salmonella in watershed were qualitatively and quantitatively detected by multiplex PCR and qPCR (chapter 2). Since Salmonella species are commonly present in poultry and poultry products as well as most popular foodborne pathogen in …

Feed Supplementation With Natural Extracts Of Cranberry And Its Efficacy On Campylobacter Colonization In Poultry, Ann Woo-Ming

Graduate Theses and Dissertations

Campylobacter spp. has been identified as one of the leading causative agents of food borne diarrheal illness. Epidemiological evidence has shown that poultry is the main source for human infection. Poultry are asymptomatic carriers of Campylobacter within their gastrointestinal tract, with colonization levels reaching 106-108 cfu/g cecal content. Surveys of domestic poultry flocks have estimated that approximately 90% of flocks are positive for Campylobacter colonization. Risk assessment studies have determined that by reducing levels of Campylobacter colonization during rearing, incidences of human infection will be significantly reduced. Currently there are no consistently effective treatments to eliminate Campylobacter from poultry flocks. …

Investigation Of In-Package Ozonation: The Effectiveness Of Ozone To Inactive Salmonella Enteritidis On Raw, Shell Eggs, Austin Donner

The Journal of Purdue Undergraduate Research

Food production, handling, and distribution practices pose a constant threat to the quality and safety of food products. The objective of this research is to evaluate an innovative in-package ozonation process to reduce Salmonella enteritidis on raw, shell eggs. Previous research has shown that in-package ozonation eliminates contaminants from outside sources, reduces pathogens, and extends shelf life. In this study, raw, shell eggs were inoculated with Salmonella enteritidis and exposed to ozonation treatment. Microbial recoveries were then tested to determine bacterial reductions. Measurements included: relative humidity (34 percent at 5^oC), surface temperatures (^oC), ozone concentrations, bacterial …

The Antimicrobial Activity Of Cold Pressed Terpeneless Valencia Orange Oil At Cold Temperatures, Sean Jeffrey Pendleton

Graduate Theses and Dissertations

This research was undertaken to identify an antimicrobial for use during chilling in beef processing, including periods of temperature abuse, as well as to evaluate membrane filtration as means to remove essential oils from bacteria to prevent further antibacterial action. Cold pressed terpeneless Valencia orange oil was examined in combination with various temperatures (37°C, 10°C, and 4°C) to determine its antimicrobial activity against various strains of bacteria. The strains were tested using a ninety-six well microtiter plate method, with or without membrane filtration. The compound 2, 3, 5-triphenyl tetrazolium chloride was used as a growth indicator. Serial, two fold dilutions …

Letter From The Dean, Lalit Verma

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Investigating The Effectiveness Of Malic Acid, Nisin, And Grape Seed Extract Incorporated Into Whey-Protein Coatings To Inhibit The Growth Of Listeria Monocytogenes On Ready-To-Eat Poultry, Amanda Bettasso, Navam Hettiarachychy, Vidya Chitturi, Michael Johnson

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

The ability to control growth of Listeria monocytogenes on ready-to-eat poultry products with the antimicrobials nisin, malic acid, and grape seed extract incorporated into whey-protein coatings was evaluated. The antimicrobials were incorporated into the coating solution alone and in combinations. One gram pieces of turkey frankfurters were coated with the coating solutions and then inoculated with L. monocytogenes and stored at 4°C for 28 days. The inhibitory effect of the coatings on turkey frankfurter pieces was evaluated on d 0, 7, 14, 21, and 28. Coatings containing 2% malic acid, 3% malic acid, and the combination of nisin (6,000 IU/g) …

Effects Of Pre-Milking Teat Sanitation On The Quality Of Raw Milk, Laurie Depiazzi, Ian Bell

Bulletins 4000 -

The effect of Quartermate pre-milking teat sanitation on milk quality was investigated in twelve dairy herds over a period of eight weeks. Pre-milking teat sanitation was carried out on all cows for six weeks on each of six farms and mesophile and thermophile bacteria counts of raw milks were compared before the milk line (“cluster” samples), and at various points within milking plants. Rates of mastitis, vat milk iodide levels and specific bacteria were also monitored.

Food Habits Of Salmo Trutta And Salvelinus Fontinalis (Pisces: Salmonidae) In Relation To Seasonal Changes And Mitigative Liming Effects In Dogway Fork Of The Cranberry River, West Virginia., Jason A. Morgan

Theses, Dissertations and Capstones

Dogway Fork, a tributary of the Cranberry River in West Virginia, is acidic due to acid precipitation and poorly buffered soils. This study is part of a long-term investigation involving the effects of continuous limestone neutralization on the fishes and benthos of an acid stream. Prior to treatment in 1988, the stream had a pH of 4.5 and there were no fish present. Since treatment began, the pH of the stream has risen to 6.8. Nine species of fish have been collected and six species have reproduced following the improvement in water quality. Densities of specific acid-sensitive benthic macroinvertebrates have …

Uht Milk : Expanding The Market, Caroline Love, Ian Bell, Martin Robertson

Journal of the Department of Agriculture, Western Australia, Series 4

An increase in the export of UHT (Ultra Heat Treated) milk to Asian markets is potentially worth millions of dollars to the dairy inmdustry. This increase however, relies on the shelf life of UHT products being extended to nine months. Spoilage of UHT milk can be caused by bacterial spores which originate on-farm and are resistant to processing. Expansion in the UHT market therefore relies on a continued reduction in the number of these spores getting into raw milk. Caroline Love, Ian Bell and Martin Robertson report on the sources of spores entering milk on-farm and the control methods available.

Mastitis : Its Prevention And Control. 1. The Udder In Health And Disease, F C. Wilkinson

Journal of the Department of Agriculture, Western Australia, Series 4

This is the first of a series of four articles on mastitis which will appear in consecutive issues of the Journal of Agriculture.

This is an introductory article describing the cow's udder in health and disease.

Tests For The Determination Of Milk Quality, H Maslin

Journal of the Department of Agriculture, Western Australia, Series 4

Foolproof tests devised to determine the quality of milk delivered to the factories can detect sub-standard milk with accuracy.

They have an important place in helping achieve a high standard of quality in our dairy products.

Water Cooling Towers : For Cooling Milk On The Dairy Farm, K Needham

Journal of the Department of Agriculture, Western Australia, Series 4

Experience on dairy farms where water cooling towers have been installed has shown that the quality and grading of milk and cream has been improved and maintained, particularly during the summer.

MOST defects in the quality of dairy produce can be attributed to the activity of micro-organisms which cause spoilage.