Food Science Commons^™

Comparison Of Allergen Legislation Between India And Ireland, Naresh Machha

Theses

2 Abstract Food allergen is one of the major issues in countries like Ireland and India. In Ireland, allergen and ingredients labelling are considered as the topmost problems in the Irish food business. The problems can be solved under a strict framework guided by strong regulations. The problem is bigger in India than Ireland as in India, there is no proper legalized standard in the context of food allergens so the people who are allergic to certain food products are on high risk. Labelling is a major issue in India due to pluralism and diverse linguistic barriers. Ireland operated FSAI …

A Study Of Consumer Perception Of Antibiotic Resistance And Antibiotic Use In Ireland, Andrea Todea

Theses

The purpose of this study was to assess the knowledge of Irish consumers regarding antibiotic use in Ireland. Data was obtained on antibiotic use, frequency, associated source and general awareness regarding antibiotics and antibiotic resistance. A cross-sectional survey was designed to analyse consumer’s perception and knowledge of antibiotic and antibiotic resistance. A total of 763 completed questionnaires were collected. The questionnaire was targeted for general public, based in Ireland. Fifty-seven percent of respondents are trying to completely avoid antibiotics, unless absolutely necessary. Half of respondents (50%) took antibiotics in the last 12 months. The majority of respondents obtained antibiotics through …

Investigation Of Consumer Attitudes Towards Emerging Novel Food Processing Technologies In The Republic Of Ireland, Emine Berna Cibik

Theses

Food industry and governmental organisations are continuously working on innovative food processing technologies in order to produce safe foods without causing undesirable changes in the food products. Food is a very sensitive area so consumers are conservative in accepting food products produced from novel technologies compared to other products. Food is basic needs of humans and it is becoming more and more globalized recently, but public perceptions are not same in different countries. The aim of this research was to investigate consumer attitudes toward novel food processing technologies in Republic of Ireland. A quantitative research was conducted and data was …

An Investigation Into The Prevalence Of Food Hypersensitivity Amongst Children In The Early Years Service In Ireland, Ruta Patel

Theses

In the early years services in Ireland, the study studied the prevalence of hypersensitivity to food and the associated food among children between the aging of 0 and 12. Participants were 980 EYS recorded with the State and Department of Education of the Republic of Ireland and Northern Ireland during 2019. In the descriptive-analytical report, the management guideline for answering these questions by the EYS was circulated electronically via emails and included information regarding class, age, food hypersensitivity and food that induce hypersensitivity among children. Results on the prevalence of allergens to Food and Coeliac diseases and current policies to …

Food Proteins: Sustainable Sources Of Proteins Responsible For Emulsions Stability In Food Products, Gleicy Kelly Oliveira Nogueria

Theses

One of the major challenges manufactures are facing is the fact that consumer’s personal health standards are changing constantly, modern consumers are willing to know more about the ingredients used in their food. The relationship with ethics and sustainability is leading to changes in the food industry. Seen this, manufactures are opting for other sources of ingredients, such as plant-based alternatives. However, the use of these sort of products need to be developed due to of their unknown properties. The use of sustainable plant proteins provides a variety of benefits to the product, such as added nutritional value as fiber, …

Prevalence, Antibiotic-Resistance And Growth Profile Of Vibrio Spp., Isolated From Imported Fish In The Local Markets, Tarfa Ali Mohamed

Theses

Seafood and fish are important food components for a large section of the world population. Seafood is prone to bacterial contamination, many are pathogenic to human and marine animals, and three species, Vibrio mimicus, Vibrio parahaemolyticus, and Vibrio vulnificus, are responsible for most cases of seafood-related human illness caused by Vibrio species. The study on the prevalence of these microorganisms in seafood of the United Arab Emirates is vital due to the cultural background of the Emiratis as a coastal heritage. A study was conducted to assess the prevalence of Vibrio spp. in imported fishes from local …

Evaluation Of Phytochemical Composition And Anti-Cancer Potential In Root Extracts Of Moringa Peregrina (Forssk.) Fiori, Salama Khamis Sultan Saeed Al Kaabi

Theses

Natural products provide many bioactive lead molecules for the discovery of new medicines. Naturally, derived phytochemicals have exhibited tremendous biological activities including anticancer activity. More than 60% of antitumor medicines are closely associated with natural products. In the present study, hexane, chloroform, acetone and, methanol extracts from roots of Moringa peregrina were screened for phytochemical analysis and anticancer activity. Phytochemical analysis was performed by Gas Chromatography and Mass spectrometry (GC-MS). The anticancer potential of the extracts was done on the human breast cancer cell line (MCF-7) and cell viability was measured using WST-1 Cell Proliferation Assay Kit. The cell line …

Hyperspectral Imaging Evaluation Of The Freshness Of Mushrooms (Agaricus Bisporus), Meera Hmoud Saeed Aldhaheri

Theses

Hyperspectral imaging (HSI) is a non-destructive analytical tool that can be used for sensing multiple attributes of foods. This thesis evaluates the application of HSI for measuring the freshness of the mushroom Agaricus bisporus (A. bisporus) in comparison with traditional seed methods. Three separate experiments were performed with 135 mushroom samples stored either in packed or unpacked conditions for 11 days. The overall results suggest that the HSI spectral region between 400 and 1000 nm is suitable for the inspection of mushroom freshness. Two distinguishing variables that aligned with physicochemical and microbiological analysis were recognized. Moisture loss was the main …

Fat Uptake Reduction In Deep-Fried Chicken Breast, Lovie G. Matthews

Theses

Obesity is the deposit of excessive adipose tissue on the body due to the overconsumption of nutritionally inadequate foods. The fat in the deep-fat fried chicken is derived from the oil absorption during frying. High consumption of deep-fat fried foods may lead to cardiovascular diseases and obesity. The main objectives of this experiment were to reduce the fat uptake in deep-fat fried chicken breast by developing an edible coating made of chicken breast protein isolates (CPI) as well as replace the corn starch in batter with sweet potato starch to further examine the quality attributes of the battering systems. In …

Isolation, Identification And Characterisation Of Enterococcus Faecalis Isolates For The Generation Of Multifuctional Bioactive Peptides From Milk, Kenneth Patrick Graham

Theses

Enterococci are lactic acid bacteria (LAB) that have the paradoxical position of possessing biochemical properties of technological importance, but are also considered as opportunistic pathogens capable of causing infection in immunocompromised patients. However, they have a long history of safe use in foods and have been evaluated in several studies for their role as starter, adjunct, probiotic and protective cultures. They show higher proteolytic activities than other LAB and have demonstrated the ability to produce bioactive peptides during the fermentation of milk. The aim of this project was to identify proteolytic Enterococcus faecalis isolates capable of generating health-promoting bioactive peptides …

A Modified Rcm Medium For The Growth Of Lactobacillus Bulgaricus, Ayowole Caleb Oyeniran

Theses

In this study, we modified reinforced clostridial medium (RCM) to selectively enumerate and isolate Lactobacillus bulgaricus, a probiotic and an important starter culture in the dairy industry. The disparity in the reported carbohydrate fermentation pattern of L. bulgaricus was used to develop a growth medium not only selective for L. bulgaricus but significantly inhibitory to the growth of other lactic acid bacteria. A recently modified RCM (mRCM) was optimized for this study by the addition of 0.5% fructose, 0.5% dextrose, 1% maltose and 0.25% sodium pyruvate while replacing lactose as a carbohydrate source. The cell recovery and bacterial counts of …

Study Of Rheological, Chemical, And Microbiological Properties Of Chami, A Traditional Emirati Soft Cheese, Aysha Othman Abdullah Al Katheeri

Theses

In this work, ten Emirati Chami cheese samples were collected and analyzed to determine their chemical composition, texture, rheology, and microbiological properties. Chami cheeses showed large variations in moisture (60.9–84.1%), protein (7.5–14.6%), fat (0.5–7.8%), and ash (3.4–8.0%) contents as well as in pH (3.6–4.4), and water activity (0.977–0.999%). The variation in fat content of samples suggested that extra fat was added; this added fat was either butter or vegetable oil as indicated by the fatty acid composition. The different samples showed peaks of variable size for fat melting. The sodium content in the 10 cheese samples varied from 223–2410 mg/kg, …

Bill Owens: A Us Craft Beer Pioneer, 1982-2001, Patrick Walls

Theses

Bill Owens is a pioneer in the United States craft brewing industry through his efforts as an advocate, writer, publisher, brewer, and entrepreneur who created a lasting legacy by influencing generations of brewers and beer fans. Owens wrote the first book on homebrewing equipment (How to Build a Small Brewery: Draft Beer in Ten Days in 1982). He opened the third brewpub in the country (Buffalo Bill's Brewery in Hayward, California in 1983) where, in 1985, he introduced the first commercial pumpkin beer among other beer style firsts. Owens published numerous brewery-focused magazines that featured many illustrious beer writers. …

Nutritional Labelling Providing Education On Its Usage Could It Impact On Patient's Food Choice Selection For Those In The Obese Classification?, Celia Lane

Theses

A build-up of too much body fat can cause significant health risks and development of medical conditions and disabilities (National Institute Clinical Excellence (NICE), 2014). The causes of obesity involve the disproportionate intake of calories that are not utilised (World Health Organisation (WHO), 2016). The rising trend towards obesity threatens to bankrupt medical systems (Heifer and Shultz, 2014). There has been a heighten awareness from the general public in the last decade of the affiliation between health and nutrition (Irish Universities Nutrition Alliance (lUNA), 2011). In light of the ever increasing obesity trend, individuals still desire to take control of …

Development And Validation Of Gas Chromatographic Methods For Free Fatty Acid Determination In Dairy Products, David Mannion

Theses

Quantification of free fatty acids (FFA) in dairy products by gas chromatography flame ion'^ation detection is an important requirement for qua'ity, research, nutritional, authenticity and legislative purposes. Common procedures are typically variants of either the fatty acid methyl esterification (FAME) or direct on-column injection methods developed more than two decades ago. This study involved the development of an alternative novel derivatization method using butylation, incorporating automation, reducing solvent usage that is applicable for a wide range of dairy products. The accuracy, precision, linearity, limits of detection and limits of quantification of all three methods were assessed for the quantification of …

Listeria Monocytogenes'. A Genomic Perspective On Its Pathogenesis, Persistence And Bacteriophages., Aidan Casey

Theses

The objective of this study was to investigate the genetic characteristics of Listeria monocytogenes that contribute to the threat it poses to the food processing industry. Transcriptomic analysis of a persistent L. monocytogenes isolate following exposure to an industrial detergent identified key upregulations in biological processes involved m peptidoglycan biosynthesis, chemotaxis and motility. Two bacteriophages against serotype 4b and 4e isolates of L. monocytogenes were isolated and sequenced. Comparative genomic and functional analysis identified the receptor binding proteins in each phage, whi]e transmission electron microscopy demonstrated a conserved baseplate architecture which also exists in other Gram­positive phages. Comparative genomics of …

Exploiting Bacteriophages And Associated Peptidoglycan Hydrolases With Potential For Biocontrol In Food Related Applications, Lorraine Endersen

Theses

The work presented in this thesis describes the isolation and characterisation of novel bacteriophages, and the subsequent exploitation of their bactericidal properties against two opportunistic food related pathogens, Mycobacterium avium subsp. paratuberculosis (MAP) and Cronobacter sakazakii.

MAP is a proven animal pathogen known to cause Johne’s disease in cattle but has been implicated as a causative agent of Crohn’s disease in humans. Despite the fact that this association has yet to be proven, significant focus has been directed towards evaluating the consequences of consuming milk contaminated with MAP. Accordingly, six mycobacteriophages were isolated and characterised in terms of temperature and …

Studies On The Initial Stage Of Lipid Oxidation In Bulk Oils, Elizabeth Budilarto

Theses

During the oxidation of bulk oils, oxidation products (i.e. peroxide values, conjugated dienes and thiobarbituric acid reactive substances) are formed gradually and increased sharply at the end of the induction period. Tocophero1s were consumed, some water was formed, and micelles increased in size during the induction period of vegetable oils oxidized in bulk. The evidence that the evolution of micellar size was in parallel with the end of induction period corroborates the recognition that micelles are the active site of oxidation. The interaction of α-tocophero1 and three synergists: ascorby1 palmitate, phosphatidy1choline and L-lysine were studied in cod liver oil, to …

Development Of Value Added Products From Spent Kluyveromyces Marxianus Yeast, Rahul Sharma

Theses

Biochemical analysis of spent Kluyveromyces marxianus yeast confirmed the following composition on a dry matter basis: 43.16 % crude protein, 34.56 % total carbohydrate, 7.02 % RNA, and 13.12 % ash content. Carbohydrate components were shown to consist of 20.97 % β-glucan, 9.76 % mannan and 1.21 % chitin while protein characterisation confirmed this yeast’s protein to be a good source of essential amino acids. Total nitrogen, α-amino nitrogen, and descriptive sensory analysis was carried out on yeast extracts derived from yeast autolysis processes carried out at different pH and temperature values. Yeast extract obtained at 50ºC, pH 7.0 was …

Utilisation Of Spent Dairy Yeast Kluyveromyces Marxianus For The Production Of Flavoured Yeast Extracts., Brendan Mccarra

Theses

Spent dairy yeast Kluyveromyces marxianus (SDYK), a by-product of whey to ethanol fermentations, was investigated as a natural biomass source for the production of alternative flavoured yeast extracts. SDYK which was characterised by a high protein content (55.3% (w/w) ± 0.8) and comparable levels of flavor enhancing amino acids to that of S. cerevisiae was prepared for induced autolysis at different pH values in the presence of inductors sodium chloride and the proteolytic enzyme papain. An induced autolysis process was developed for the production of flavoured yeast extracts in which pH and autolytic treatment applied were found to have a …

Investigation Of Factors Influencing Trichloromethane Levels In Milk And Its Control., Siobhan Ryan

Theses

Trichloromethane (TCM) is an undesirable chemical residue that can develop in milk. While TCM levels in milk are not within ranges of public health concern, it is important that minimal levels are maintained in order to promote the marketing of Irish dairy products abroad. Germany has enacted strict regulations on TCM concentrations in foodstuffs with a recommended level of0.002 mg/kg and re-analyse those milks, subsequent to cause identification and provision of advice on reducing TCM levels in milk. Milk samples were analysed using Gas chromatography (GC) with electron capture detection. The GC method was adapted and a standard curve was …

Evaluation Of Novel Low Molecular Weight Antimicrobial Compounds Produced By Lactic Acid Bacteria., Jiahui Leona Guo

Theses

Fungal food spoilage plays a pivotal role in the deterioration of food and feed systems and some fungi are also able to produce compounds toxic for humans and animals. The mycotoxins produced by fungi can cause serious health hazards and include toxins which are cancerogenic, immunotoxic, teratogenic, neurotoxic, nephrotoxic or hepatotoxic effects and Kashin-Beck disease. In addition to this, fungal spoilage/pathogens cause losses of marketable quality and hygiene of foodstuffs, resulting in major economic problem throughout the world. Nowadays, food spoilage can be prevented using physical and chemical methods, but no efficient strategy has been proposed so far to reduce …

Development Of Pcr Assays To Detect And Characterise Salmonella Isolates From Cheese And Meat Products By Utilizing An Integrated Genomics Approach, Noelle Geraldine Mccarthy

Theses

Salmonella is an extremely important foodborne pathogen which is responsible for millions of cases of gastroenteritis annually. Salmonella outbreaks have been associated with numerous common food stuffs such as meat, dairy and seafood. Traditional culture based methods for the detection Salmonella are labourious and costly. Rapid detection and serovar identification would dramatically decrease the time required to identify Salmonella outbreaks and potential health risks.

In the US 99% of all Salmonella infections are caused by members of Salmonella enterica subspecies 1. Serovars within subspecies 1 can vary due to host specificity and pathogenicity. DNA sequencing projects have provided valuable genomic …

System Identification Of A Pasteurisation Process: A Comparison Of Direct And Indirect Methods, Niel Canty

Theses

The primary focus of this research is to identify a linear model for a direct steam injection (DSI) pasteurisation process which is a component of a large-scale pilot evaporation process widely used in the dairy industry. The pasteurisation process has been identified as a key component of the evaporation process. The author believes that no previously published work has focused on identifying models for a DSI pasteurisation process which makes this work an original contribution to the area. The pasteurisation process can be modelled as a single-input, single-output (SISO) process with a measurable disturbance. Thus two transfer functions were required …

The Development Of Functional Food Ingredients From Yeast Cell Wall Beta Glucans, Susan Keogh

Theses

The intracellular contents of spent yeast can be used to produce flavours and flavour enhancers. However there still remains after this process, a significant quantity of cell wall material, which makes up 20-25% of the dry weight of yeast biomass. A major component of the yeast cell wall is the polysaccharide P-glucan. This project involved the investigation of the extraction and characterisation of p-glucan as well as its evaluation as a functional food ingredient. A preparation of spent yeast p-glucan was obtained from two types of spent yeast species, Saccharomyces cervisiae (brewers yeast) and Kluyveromyces marxianus (dairy yeast) obtained from …