Food Science Commons^™

Mechanisms Of Hard-To-Cook Defect In Cowpeas: Verification Via Microstructure Examination, Keshun Liu, Yen-Con Hung, R. Dixon Phillips

Food Structure

It has been hypothesized that part of the hard-to-cook (HTC) defect in cowpeas is due to decreases in solubility and thermal stability of intracellular proteins during storage since coagulated proteins would limit water to starch and prevent full swelling during cooking. To verify this hypothesis, effects of soaking and heating temperature on texture, water absorption, protein water extractability and microstructure of control and HTC cowpea seeds (aged or treated) were studied. Scanning electron microscopy showed no structural difference between dry control and dry aged seeds. However, after soaking, in contrast to the control, aged seeds exhibited a coarse protein matrix …

An Apparatus For A New Microcube Encapsulation Of Fluid Milk In Preparation For Transmission Electron Microscopy, M. C. Alleyne, D. J. Mcmahon, N. N. Youssef, S. Hekmat

Food Structure

A simple apparatus has been developed for a new "microcube" encapsulation of fluid milk samples in their prefixation preparation for electron microscopy. The new technique is based on making cubic wells in an agar gel layer, filling them with fluid milk samples, and sealing them with another agar gel layer . The individual wells are then separated by cutting from the initial block, providing 0.5 mm walls around the samples. The embedded material (milk, buttermilk, yogurt, etc.) is fixed, dehydrated , and embedded in a resin for transmission electron microscopy. The procedure is simpler, and more versatile, reliable, and reproducible …

Whey Proteins As Microencapsulating Agents. Microencapsulation Of Anhydrous Milkfat - Structure Evaluation, M. Rosenberg, S. L. Young

Food Structure

Microencapsulation of milkfat may open new fields of application for this milk constituent by transforming it into dry and stable powder. Research has been undertaken to study the microstructure of whey protein based, anhydrous milkfat-containing, spray-dried microcapsules. Whey protein concentrates of 50% and 75% protein and whey protein isolate were evaluated as microencapsulating agents (wall materials). The effects of wall composition, fat load , and drying conditions on the capsule's structure were studied by scanning electron microscopy (SEM). Spherical capsules with smooth , wrinkle-free surfaces were observed in all cases. Whey protein isolate-based microcapsules dried at a temperature higher than …

Microstructural Studies In Fat Research, I. Heertje

Food Structure

Microstructural studies play an important role in establishing the relation between composition, processing and final properties of many food products. In order to arrive at a full description of microstructure many visualization- and preparation techniques are needed. A number of fatty products such as shortenings, margarine , butter, and low fat spreads are discussed from a microstructural point of view. Examples of the influence of process parameters on microstructure and rheological properties are given. In particular, attention is paid to the fat crystalline matrix and the emulsion structure.

Further, a new methodology is described making it possible to study interactions …

The Stabilisation Of Air In Foods Containing Fat - A Review, B. E. Brooker

Food Structure

Foods containing aqueous solutions of proteins readily foam when air is introduced into them. When fat is also present, interaction between the two components at the air/water interface may produce a stable foam with characteristic bulk properties. In the case of dairy foams (such as whipped cream), bubbles produced in the whipping process are initially stabilised by the adsorption of protein at the air/water interfaces.

Commonly encountered defects in whipping cream arise when large triglyceride crystals, formed in masses of free fat, adsorb to the air/water interface during whipping at the expense of fat globules.

In other food systems , …

Microstructure And Rheological Properties Of Composites Of Potato Starch Granules And Amylose: A Comparison Of Observed And Predicted Structures, K. Svegmark, A.-M. Hermansson

Food Structure

Potato starch granules were gelatinised in amylose solution to study the effect of adding amylose to a highswelling granular starch system. The effects of varying the amount of potato starch from 1-10% , added to a solution of 2% amylose, were studied by means of dynamic viscoelastic measurements and light microscopy.

The granules gelatinised in amylose solution had a lower degree of swelling than those gelatinised in water. The restricted swelling in amylose was reflected in a decrease in the complex shear modulus (G*) at 75'C. GeJatinisation in 2% amylopectin also caused a decrease in G*, but gelatinisation in 2% …

Relationship Between The Starch Granule Structure And The Textural Properties Of Heat-Induced Surimi Gels, V. Verrez-Bagnis, B. Bouchet, D. J. Gallant

Food Structure

Starch, used as a textural additive in heat-induced surimi gel, influences the rigidity of the protein gel matrix and hence the gel strength according to its botanical characteristics. The present study focuses on the correlations existing between the textural properties of heatind uced surimi gels obtained by physical measurements and the characteristics of different commercial starches. The gelatinization temperature of starch was closely related to the expressible moisture, work to fracture, and elongation. Behaviour of starchy components during thermal processing and its relationship to fish protein gel matrix were studied by light and electron microscopy. These studies showed differences in …

Structure-Compressive Stress Relationships In Mixed Dairy Gels, J. M. Aguilera, J. E. Kinsella, M. Liboff

Food Structure

Mixed dairy gels (including a control without fat) of skim milk powder (SMP) and whey protein isolate (WPI) containing fat globules were formed by heating protein emulsions to 90°( and by acid release from glucono- 0-lactone to provide a pH of 4.3-4.4 . Fat globules with artificial protein membranes (FGAPM) were prepared by homogenization of a butter oil /water mixture in the presence of WPI while fat globules without membranes were stabili zed with polyoxyethylene sorbitan monolaurate (Tween 20). Both emulsions were added at a 4% (w/w) leve l to solutions having 3% SMP and 8.3% WPI. The gel contai …

A Tutorial And Comprehensive Bibliography On The Identification Of Foreign Bodies Found In Food, D. F. Lewis

Food Structure

The paper presents a philosophical approach to the investigation of foreign body incidents in food. The suggested procedure has similarities with "key" procedures used for taxonomy. However, the wide diversity of materials encountered and the need to determine something of the history of the offending item demands modifications to this process. The categorisation may be made on instinct or experience, and appropriate confirmatory tests will be applied in the light of the initial categorisation. Cardinal rules are proposed: firstly , the receipt of the items should be well documented; and secondly, the initial observations should be as non-destructive as possible. …

Practical Aspects Of Electron Microscopy In Dairy Research, Miloslav Kalab

Food Structure

Milk products are based mostly on casein micelles, fat globules, and whey proteins. The former two constituents are corpuscular and whey proteins become corpuscular when coagulated. Structural changes in these basic constituents during processing have been studied by electron microscopy. This review discusses the structures of yoghurt, curd, cheeses (hard cheeses, mould-ripened cheeses, cream cheeses, and process cheese), cream, milk powders, and nontraditional dairy products. Defects and deviations from traditional structures of these products are explained where the causes are known. Examples of such causes are foaming of milk, presence of unusual ingredients (bacterial polysaccharides, whey protein concentrates), and alterations …

Fluidization And Its Applications To Food Processing, N. C. Shilton, K. Niranjan

Food Structure

This paper is a comprehensive review of the science behind fluidization of food materials, and its applications in food processing. Fluidization is a process by which a bed of particulate materials exhibts fluid -like behaviour as a result of fluid flowing through it. Fluidization can be carried out by liquids or gases and different forms of fluidization occur depending on the type of fluidizing medium and the properties of the particulate material , this can have an important effect on the type of processes that can be carried out using fluidization.

Typical food processing applications of fluidization include freezing and …

Hydrocolloids As Food Emulsifiers And Stabilizers, Nissim Garti, Dov Reichman

Food Structure

Hydrocolloids are water-soluble biopolymers consisting of high molecular weight polysaccharides known as viscosity builders, gelification agents and stabilizers of food systems.

Several hydrocolloids such as gum arabic (acacia), tragacanth, xanthan and certain modified gums have been mentioned as food additives having special functions such as: "retardation of precipitation of dispersed solid particles and coalescence of oil droplets". The role of these gums as emulsifiers remained somewhat obscure. The present review is an attempt to bring the relevant studies together and to throw some light on the functionalities of the gums as surface active agents and food emulsifiers. In addition, some …

A Low-Temperature Scanning Electron Microscopy Study Of Ice Cream. Ii. Influence Of Selected Ingredients And Processes, K. B. Caldwell, H. D. Goff, D. W. Stanley

Food Structure

The objective of this study was to examine the influence of processing parameters, viz., incorporation of polysaccharide stabilizers, freezing rates, and storage times and temperatures on the microstructure of ice cream. As the freezing rate was reduced, ice crystals and air bubbles increased in size. However, quenchfreezing also contributed to poor texture; thus an optimum freezing rate exists for the production of suitably- sized ice crystals and texture in ice cream. Model systems of polysaccharide stabilizer solutions were seen to have a characteristic network structure when quenchfrozen which was altered by the addition of sucrose. Stabilized ice cream initially had …

Effect Of Draw Ph On The Development Of Curd Structure During The Manufacture Of Mozzarella Cheese, L. J. Kiely, P. S. Kindstedt, G. M. Hendricks, J. E. Levis, J. J. Yun, D. M. Barbano

Food Structure

The impact of varying the pH of whey at whey drainage (5.9, 6.15 or 6.4) on the development of curd structure during the manufacture of Mozzarella cheese was investigated using scanning electron microscopy. Dramatic changes in curd structure were apparent with stage of manufacture , in particular the stretching step which aligned the protein fibers.

Of additional interest is the effect of draw pH on the structure of curd at whey drainage. When whey was drained at pH 6.4, an open poorly fused network of paracasein particles was observed in the cheese curd. Jn contrast, lowering the draw pH (6.15 …

Ultrastructural Changes In Cherimoya Fruit Injured By Chilling, Margarita Gutierrez, Maria Del Mar Sola, Luis Pascual, Maria Isabel Rodriguez-Garcia, Alberto M. Vargas

Food Structure

Cherimoya (Annona cherimola Mill.) is an important fruit crop that is grown in the South of Spain. Ultrastructural modifications of cherimoya fruit were studied after low-temperature storage. When cherimoya was stored at 4 oc for 6 days, the starch grains did not suffer degradation and the cell walls remained intact. The membrane systems were severely damaged , result ing in a loss of cell compartmentalization. Cherimoya rewarmed to 22 0 C after 9 days of low temperature storage is not able to recover , showing the irreversibility of the ultra structural changes. In addition, disorgani zation of the internal lamella …

Thermal Processing Effects On Rice Characteristics, Shuh-Ming Chang, Hsiu-Chen Yang

Food Structure

Whole kernels of four cultivars of milled rice were treated under different conditions according to our traditional methods such as boiling, steaming, autoclaving or roasting with sand. During the vari ous processing conditions, the moisture contents of the kernel , heating temperatures, pressures and heating times were varied. In this paper, the physicochemical properties of those processed samples were investigated and compared.

Based on degree of gelatinization , water absorption index , water solubility index , swelling power, viscoamylograms and X-ray diffraction patterns, the propenies of waxy rice samples arc different from that of non -waxy rice samples. They are …

A Moving Optical Fibre Technique For Structure Analysis Of Heterogeneous Products: Application To Different Foodstuffs, J. C. Akkerman, C. G. J. Bisperink, A. D. Ronteltap

Food Structure

The macro structure of many soft heterogeneous products can be studied by a moving optical fibre technique . The method i s based upon an optical sensor that penetrates into the heterogeneous medium. The optical sensor discriminates between materials differing in refractive index or in scattering properties. Coupling of the signal of the optical sensor to its position reveals the spatial distribution of the materials in the product. Applications shown include bread, soft ice-cream, chocolate mousse and draining curd. The major advantages of the method are its speed and ease; moreover, the sample is hardly disturbed by the sensor. The …

Objective Measurement Of The Visual Aspect Of Dry Sausage Slices By Image Analysis, A-C. Roudot, F. Duprat, M.-G. Grotte, G. O'Lidha

Food Structure

The visual aspect of food is a problem very often neglected for different reasons; one being the difficulty of modelling vision. However, it has been shown that , on simple synthetic images, automatic sorting in the same manner as human vision is possible.

Distribution heterogeneity of spots within an image is one important parameter for its characterization. A new algorithm was tested over simple numerical pictures that gave good agreement with visual appreciation. Images of twenty dry sausage slices were analyzed and different factors corresponding to aspect structure were calculated. Principal component analyst:s based on these factors all owed discrimination …

Effects Of Processing On The Microstructure Of Oat (Avena Sativa) Bran Concentrate And The Physicochemical Properties Of Isolated B-Glucans, K. Autio, Y. Malkki, T. Virtanen

Food Structure

Fluorescence microscopy was used to study the microstructure of oat cell walls during concentration of oat bran and isolation of ,B-glucans. The bran concentrate separated from c.v. Nasta contained mainly the aleurone and subaleurone endosperm layers, whereas that separated from a commercial bran mixture contained more endosperm . In contrast to Nasta, the commercial bran mixture contained ,B-glucan degrading enzymes, which survived the ,B-glucan isolation procedure. In the presence of enzymes, the solubility and yield of ,B-glucans improved but the viscosity decreased when it was measured at the same ,B-glucan concentration. For inactivation of the enzymes, 80% ethanol at 78 …

Structure And Rheology Of String Cheese, S. Taneya, T. Izutsu, T. Kimura, T. Shioya

Food Structure

String cheese samples ranging in pH from 5.0 to 5.9 were prepared by kneading Mozzarella curd and stretching it 2 to 80 times. Rheological properties were examined by compression, stress relaxation , and flow property tests. Stringiness was tested by a device specifically designed for this purpose: a standardized string was pulled from the sample at a direction perpendicular to the orientation of the curd and the amount of fibres resulting from this process was quantitated using digital image analysis.

Force-deformation curves implied that the curd prepared by kneading may be considered to be an incompressible viscoelastic body similar to …

Computer Modeling: The Adjunct Micro Technique For Lipids, J. W. Hagemann, J. A. Rothfus

Food Structure

Chemistry by computer provides access to microsystem information not readily achieved by other means. Simple computational analysis of saturated triglyceride polymorphism by molecular mechanics within constraints provided by X-ray data show that polymethylene interactions determine hydrocarbon crystal properties when they account for more than 60 percent of the total molecular interaction energy. Modeling predicts multiple , nearly equivalent, a -form triglycerides and a -form character in liquid near-crystalline triglycerides. Symmetrical molecules pack better than asymmetric molecules in a-form configurations and transform readily to 13'forms if activated sufficiently to disrupt lateral chain interaction and allow dimensional displacements during transformation. The formation …

Microstructure And Texture Of Khoa, G. R. Patil, A. A. Patel, Paula Allan-Wojtas, Miloslav Kalab

Food Structure

Khoa, a partially dehydrated milk product indigenous to India, was prepared from buffalo milk by boiling it vigorously in an open pan and reducing its volume to approximately 25% within 30 min. The hot semi-solid product (Khoa pat) was held at 20•c for 3 h (fresh, cooled Khoa) or 48 h (swred Khoa); the products were either worked with a pestle in a mortar for 5 min or were left without working.

Structural features of Khoa were studied by light microscopy and electron microscopy. Freshly prepared coo led Khoa had a granular structure consisting of protein granules several hundred micrometers …

Importance Of Enzymes To Value-Added Quality Of Foods, J. R. Whitaker

Food Structure

New uses of enzymes are of major importance for value-added quality of foods. At the production and postharvest handling levels, enzymes associated with maturation , color , texture, flavor and nutritional changes are important. Recent examples are discussed which include longer storage prior to initiation of ripening , deletion or control of genes that result in softening1 browning and other quality defects and the enhancement of genes that improve the nutritional quality of foods. At the processing level, new uses of enzymes are described to change the properties of proteins and lipids , to eliminate off-flavor in beers and sterilized …

Light Microscopy Measurements Of Ice Recrystallization In Frozen Corn Starch Pastes Using Isothermal Freeze Fixation, C. Ferrero, M. N. Martino, N. E. Zaritzky

Food Structure

Isothermal freeze fixation was used to analyze ice recrystallization by light microscopy in a 10 % (W/W) frozen corn starch paste during storage at temperatures in the range of -5 to -20 °C.

Different formulations were tested in order to obtain a suitable fixative for this method of indirect observation of the ice crystals. A solution of formaldehyde, ethanol and water (10:45:45 V:V) was selected because it minimized substitution-induced distortion and contraction of the matrix. The diffusion coefficients of the selected fixative in the frozen system were measured at different temperatures in conditions of unidirectional mass transfer in a semi …

Scanning Electron Microscopy And Ultraviolet Absorption Microspectrophotometry Of Plant Cell Types Of Different Biodegradabilities, D. E. Akin, L. L. Rigsby

Food Structure

Phenolic components within cell walls are the greatest limitation to biodegradation of plants, but the location and types of compounds within specific walls are poorly understood . Leaf blades of warm-season (Coastal and Coastcross-1 bermuda grasses and sudangrass) and cool-season grasses (orchardgrass and tall fescue) and leaflets of legumes (alfalfa and lespedeza) were evaluated before and after incubation with rumen microorganisms for 7 days to determine cell types and wall structures most resistant to biodegradation. UV absorption microspectrophotometry was used to characterize aromatics within selected undegraded cell types, which had been shown in digestion studies to vary in biodegradability, from …

Heat-Induced Structural Changes In Acid-Modified Barley Starch Dispersions, K. Autio, K. Poutanen, T. Suortti, E. Pessa

Food Structure

The effect of heat treatment on the gel formation and microstructure of barley starch dispersions , hydrolyzed with I M HCI for 0.5, 1.0 and 4.0 hours , was studied by dynamic viscoelastic methods and by light microscopy . The effects of acid hydrolysis on the molecular weight of amylopectin and amylose were studied by high -performance liquid chromatography (HPLC) with post-column iodine staining.

Microstructural studies of 8% hydrolyzed barley starch dispersions heated to 90 0 C showed that even a short acid treatment induced considerable changes in the granule structure. The molecular weight of amylopectin decreased substantially. As the …

Effect Of Ionizing Y-Radiation On Thermoluminescence And Electron Spin Rosonance Intensities In Milk Protein Concentrate Powders, J. Kispeter, L. I. Horvath, L. I. Kiss

Food Structure

Milk protein concentrate powder has found a wide application as a food ingredient. We investigated the effects of ionizing ')'-radiation at doses ranging from 2 to 20 kGy on electron spin resonance and thermoluminescence intensities in samples of milk protein concentrate powder with varying protein contents (36-73 %wt), containing addltional Fe++ ions (12-910 ppm) and stored under different conditions.

Electron spin resonance and thermoluminescence intensities increased unambiguously with absorbed -ydose. Added Fe++ ions showed a quenching effect as measured by both methods. Storage conditions affected strongly the decay of electron spin resonance signal intensity . Lineshape analysis of thermoluminescence curves …

Structure Of Margarines Made With Low Erucic Acid Rapeseed Oil, J. Hojerova, S. Schmidt, J. Krempasky

Food Structure

Margarine based on hydrogenated low erucic acid rapeseed oil shows a strong tendency to produce an unacceptable grainy structure. This is caused by rapid transition of the B-form into the B-form of fat crystals. Sorbitan esters, monoacylglycerols, and a blend of acylglycerols were investigated as possible B-stabilizers. Polymorphic transitions were evaluated by visual assessment, examination by polarized light microscopy, and x-ray diffraction analysis in margarines prepared on a laboratory scale and containing varying amounts of stabilizers. The evaluation was extended over a 7-week period of storage at 10 C. Sorbitan esters of fatty acids were found to be the most …

Microstructural Differences Among Adzuki Bean (Vigna Angularis) Cultivars, Anup Engquist, Barry G. Swanson

Food Structure

Scanning electron microscopy (SEM) was used to study microstructural differences among five adzuki bean cultivars: Erimo, Express, Hatsune, Takara and VBSC. Seed coat surfaces showed different patterns of cracks, pits and deposits . Cross-sections of the seed coats revealed well organized layers of elongated palisade cells followed by many layers of amorphous parenchyma cells. Typical sub-epidermal layers of organized columnar, hour-glass cells were characteristica11y absent in the five cultivars of adzuki beans. The cross-sections of the cotyledons revealed intercellular materials between storage cells. Storage cells in Hatsune cultivar were loosely packed with many intercellular spaces as compared to the other …

Scanning Electron Microscopy Structure And Firmness Of Papain Treated Apple Slices, Yaguang Luo, Max E. Patterson, Barry G. Swanson

Food Structure

'Mcintosh' apple (Malus domesrica Borkh.) slices were treated with papain. Textural changes were recorded with an Instron Universal Testing Machine. Structural changes and distribution of microorganisms in apple tissues after treatment were observed with a scanning electron microscope (SEM). Apple slices submerg ed in a 1% papain solution were significantly firmer than apple slices submerged in the distilled water control for a 24 hour period (P < 0.05). Three and four days after slicing , a significantly smaller decay index was observed on the apple slices submerged in papain solution than on the control slices. Under SEM, less severe cell wall breakdown was observed on the apple tissues treated with papain than on apple tissues without treatment. Less spores were also observed on the papain treated apple slices than apple slices without treatment. Apple tissues treated with papain solution and distilled water also demonstrated noticeable st ru ctural differences. The apple tissues treated with papain solution for 18 hours retained the original cell structure while the cells in the apple tissues treated with distilled water collapsed.