Food Science Commons^™

Disparate Metabolic Responses In Mice Fed A High-Fat Diet Supplemented With Maize-Derived Non-Digestible Feruloylated Oligo- And Polysaccharides Are Linked To Changes In The Gut Microbiota, Junyi Yang, Laure B. Bindels, Rafael R. Segura Munoz, Inés Martínez, Jens Walter, Amanda Ramer-Tait, Devin J. Rose

Department of Food Science and Technology: Faculty Publications

Studies have suggested links between colonic fermentation of dietary fibers and improved metabolic health. The objectives of this study were to determine if non-digestible feruloylated oligo- and polysaccharides (FOPS), a maize-derived dietary fiber, could counteract the deleterious effects of high-fat (HF) feeding in mice and explore if metabolic benefits were linked to the gut microbiota. C57BL/6J mice (n = 8/group) were fed a low-fat (LF; 10 kcal% fat), HF (62 kcal% fat), or HF diet supplemented with FOPS (5%, w/w). Pronounced differences in FOPS responsiveness were observed: four mice experienced cecal enlargement and enhanced short chain fatty acid production, indicating …

Pharmacological Activity Of Costus Spicatus In Experimental Bothrops Atrox Envenomation, Leide Caroline Dos Santos Picanço, José Adolfo Homobono Machado Bittencourt, Shayanne Vanessa Correia Henriques, Juliane Silva Da Silva, Juliana Maria Da Silva Oliveira, José Renato Ribeiro, Antony-Babu Sanjay, José Carlos Tavares Carvalho, Didier Stien, Jocivânia Oliveira Da Silva

Department of Food Science and Technology: Faculty Publications

Context: Medicinal plants encompass a rich source of active compounds that can neutralize snake venoms or toxins. Costus spicatus (Jacq.) Sw. (Costaceae) is used by the Amazonian population to treat inflammation, pain and other pathological manifestations.

Objective: To evaluate the influence of C. spicatus aqueous extract on edema, peritonitis, nociception, coagulation, haemorrhage and indirect haemolytic activity induced by Bothrops atrox venom (BAV).

Materials and methods: Dried and pulverized leaves were extracted with distilled water. Envenoming was induced by administration of B. atrox snake venom in Swiss Webster mice. The experimental groups consisted of BAV (at the minimum dose to induce …

The Impact Of Long-Term Dietary Pattern Of Fecal Donor On In Vitro Fecal Fermentation Properties Of Inulin, Junyi Yang, Devin J. Rose

Department of Food Science and Technology: Faculty Publications

Although the composition of the gut microbiota is of interest, the functionality, or metabolic activity, of the gut microbiota is of equal importance: the gut microbiota can produce either harmful metabolites associated with human disease or beneficial metabolites that protect against disease. The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and …

Emergence Of A Potent Multidrug Efflux Pump Variant That Enhances Campylobacter Resistance To Multiple Antibiotics, Hong Yao, Zhangql Shen, Yang Wang, Fengru Deng, Dejun Liu, Gaowa Naren, Lel Dal, Chih-Chia Su, Bing Wang, Shaolin Wang, Congming Wu, Edward W. Yu, Qijing Zhang, Jianzhong Shen

Department of Food Science and Technology: Faculty Publications

Bacterial antibiotic efflux pumps are key players in antibiotic resistance. Although their role in conferring multidrug resistance is well documented, the emergence of “super” efflux pump variants that enhance bacterial resistance to multiple drugs has not been reported. Here, we describe the emergence of a resistance-enhancing variant (named RE-CmeABC) of the predominant efflux pump CmeABC in Campylobacter, a major zoonotic pathogen whose resistance to antibiotics is considered a serious antibiotic resistance threat in the United States. Compared to the previously characterized CmeABC transporters, RECmeABC is much more potent in conferring Campylobacter resistance to antibiotics, which was shown by increased …

Sequence-Based Methods For Detecting And Evaluating The Human Gut Mycobiome, Mallory J. Suhr, Nabaraj Banjara, Heather E. Hallen-Adams

Department of Food Science and Technology: Faculty Publications

We surveyed the fungal microbiota in 16 fecal samples from healthy humans with a vegetarian diet. Fungi were identified using molecular cloning, 454 pyrosequencing and a Luminex analyte specific reagent (ASR) assay, all targeting the ITS region of the rRNA genes. Fungi were detected in each fecal sample and at least 46 distinct fungal operational taxonomic units (OTUs) were detected, from two phyla — Ascomycota and Basidiomycota. Fusarium was the most abundant genus, followed by Malassezia, Penicillium, Aspergillus, and Candida. Commonly detected fungi such as Aspergillus and Penicillium, as well as known dietary fungi Agaricus bisporus and …

Conformational Stability Of Digestion-Resistant Peptides Of Peanut Conglutins Reveals The Molecular Basis Of Their Allergenicity, Danijela Apostolovic, Dragana Stanic-Vucinic, Harmen H.J. De Jongh, Govardus A.H. De Jong, Jelena Radosavljevic, Milica Radibratovic, Julie A. Nordlee, Joseph L. Baumert, Milos Milcic, Steve L. Taylor, Nuria Garrido Clua, Tanja Cirkovic Velickovic, Stef J. Koppelman

Department of Food Science and Technology: Faculty Publications

Conglutins represent the major peanut allergens and are renowned for their resistance to gastrointestinal digestion. Our aim was to characterize the digestion-resistant peptides (DRPs) of conglutins by biochemical and biophysical methods followed by a molecular dynamics simulation in order to better understand the molecular basis of food protein allergenicity. We have mapped proteolysis sites at the N- and C-termini and at a limited internal segment, while other potential proteolysis sites remained unaffected. Molecular dynamics simulation showed that proteolysis only occurred in the vibrant regions of the proteins. DRPs appeared to be conformationally stable as intact conglutins. Also, the overall secondary …

Prebiotics And Synbiotics: Dietary Strategies For Improving Gut Health, Janina A. Krumbeck, Maria X. Maldonado-Gomez, Amanda E. Ramer-Tait, Robert W. Hutkins

Department of Food Science and Technology: Faculty Publications

Purpose of review — A wide range of dietary carbohydrates, including prebiotic food ingredients, fermentable fibers, and milk oligosaccharides, are able to produce significant changes in the intestinal microbiota. These shifts in the microbial community are often characterized by increased levels of bifidobacteria and lactobacilli. More recent studies have revealed that species of Faecalibacterium, Akkermansia, and other less well studied members may also be enriched. We review the implications of these recent studies on future design of prebiotics and synbiotics to promote gastrointestinal health.

Recent findings — Investigations assessing the clinical outcomes associated with dietary modification of the …

Multistate Product Traceforward Investigation To Link Imported Romaine Lettuce To A Us Cyclosporiasis Outbreak – Nebraska, Texas, And Florida, June–August 2013, B. F. Buss, M. V. Joshi, J. L. Dement, V. Cantu, T. J. Safranek

Department of Food Science and Technology: Faculty Publications

During June–August 2013, 25 US states reported 631 cyclosporiasis cases including Nebraska and Iowa where a regional investigation implicated common-source imported salad mix served in two chain restaurants. At least two common-origin growing fields were likely sources of contaminated romaine lettuce. Using producer- and distributor-provided data, we conducted a grower-specific traceforward investigation to reveal exposures of ill US residents elsewhere who reported symptom onset during 11 June–1 July 2013, the time period established in the Nebraska and Iowa investigation. Romaine lettuce shipped on 2–6 June from one of these Mexico-origin growing fields likely caused cyclosporiasis in 78 persons reporting illness …

Threshold Dose Distribution And Eliciting Dose Of Cashew Nut Allergy, Johanna P. M. Van Der Valk, Roy Gerth Van Wijk, J. L. Baumert, Julie A. Nordlee, Berber J. Vlieg-Boerstra, Hans De Groot, Anthony E. J. Dubois, Nicolette W. De Jong

Department of Food Science and Technology: Faculty Publications

A previous study found that 137 of 179 cashew nut sensitized children (76.5%) suspected of having cashew nut allergy had a positive double-blind, placebo-controlled food challenge (DBPCFC result), with 63 of 137 children (46%) manifesting subjective and/or objective symptoms to the lowest dose (1 mg of cashew nut protein). The primary aim of this study was to determine the distribution of threshold doses and the eliciting doses (EDs) in this population. The secondary aim was to investigate whether children who reacted to 1 mg of cashew nut (n = 63) could react to even lower doses than 1 mg (low-dose …

Regional Investigation Of A Cyclosporiasis Outbreak Linked To Imported Romaine Lettuce – Nebraska And Iowa, June–August 2013, Bryan F. Buss, M. V. Joshi, C. D. Allensworth, A. Garvey, K. Obbink, S. Mandernach, Thomas J. Safranek

Department of Food Science and Technology: Faculty Publications

A regional, multistate investigation into a June–August 2013 cyclosporiasis outbreak was conducted in Nebraska, Iowa, and neighbouring states. Cases were confirmed on the basis of laboratory and clinical findings. Of 227 cases in Iowa (n = 140) and Nebraska (n=87) residents, 162 (71%) reported dining at chain A/B restaurants – 96% reported house salad consumption. A case-control study identified chain A/B house salad as the most likely vehicle. Traceback was conducted to ascertain production lot codes of bagged salad mix (iceberg and romaine lettuce, red cabbage, and carrots) served as house salad in implicated restaurants. A single production lot code …

Allergenicity Attributes Of Different Peanut Market Types, Stef J. Koppelman, Shyamali Jayasena, Dion Luykx, Erik Schepens, Danijela Apostolovic, Govardus A.H. De Jong, Thomas G. Isleib, Julie A. Nordlee, Joseph L. Baumert, Steve L. Taylor, Hsiaopo Cheng, Sohelia Maleki

Department of Food Science and Technology: Faculty Publications

Four different market classes of peanut (Runner, Virginia, Spanish, and Valencia) are commonly consumed in Western countries, but for some consumers peanuts are a main cause of food-induced anaphylaxis. Limited information is available on the comparative allergenicity of these distinct market classes. The aim of this study was to compare allergenicity attributes of different peanut cultivars. The protein content and protein profiles were highly comparable for all tested cultivars. All cultivar samples contained the major allergens Ara h 1, Ara h 2, Ara h 3 and Ara h 6, as assessed by SDS-PAGE and RP-HPLC, although some minor differences in …

Two Clinical Isolates Of Mycoplasma Hyosynoviae Showed Differing Pattern Of Lameness And Pathogen Detection In Experimentally Challenged Pigs, João Carlos Gomes-Neto, Matthew Raymond, Leslie Bower, Alejandro Ramirez, Darin M. Madson, Erin L. Strait, Everett L. Rosey, Vicki J. Rapp-Gabrielson

Department of Food Science and Technology: Faculty Publications

Mycoplasma (M.) hyosynoviae is known to colonize and cause disease in growing-finishing pigs. In this study, two clinical isolates of M. hyosynoviae were compared by inoculating cesarean-derived colostrum-deprived and specific-pathogen-free growing pigs. After intranasal or intravenous inoculation, the proportion and distribution pattern of clinical cases was compared in addition to the severity of lameness. Tonsils were found to be the primary site of colonization, while bacteremia was rarely detected prior to the observation of clinical signs. Regardless of the clinical isolate, route of inoculation, or volume of inocula, histopathological alterations and tissue invasion were detected in multiple …

Identification And Phylogeny Of The First T Cell Epitope Identified From A Human Gut Bacteroides Species, Maria Elisa Perez-Muñoz, Payal Joglekar, Yi-Ji Shen, Kuan Y. Chang, Daniel A. Peterson

Department of Food Science and Technology: Faculty Publications

Host T cell reactivity toward gut bacterial epitopes has been recognized as part of disease pathogenesis. However, the specificity of T cells that recognize this vast number of epitopes has not yet been well described. After colonizing a C57BL/6J germ-free mouse with the human gut symbiotic bacteria Bacteroides thetaiotaomicron, we isolated a T cell that recognized these bacteria in vitro. Using this T cell, we mapped the first known non-carbohydrate T cell epitope within the phylum Bacteroidetes. The T cell also reacted to two other additional Bacteroides species. We identified the peptide that stimulated the T cell by …

Distillation Time As Tool For Improved Antimalarial Activity And Differential Oil Composition Of Cumin Seed Oil, Valtcho D. Zheljazkov, Archana Gawde, Charles L. Cantrell, Tess Astatkie, Vicki Schlegel

Department of Food Science and Technology: Faculty Publications

A steam distillation extraction kinetics experiment was conducted to estimate essential oil yield, composition, antimalarial, and antioxidant capacity of cumin (Cuminum cyminum L.) seed (fruits). Furthermore, regression models were developed to predict essential oil yield and composition for a given duration of the steam distillation time (DT). Ten DT durations were tested in this study: 5, 7.5, 15, 30, 60, 120, 240, 360, 480, and 600 min. Oil yields increased with an increase in the DT. Maximum oil yield (content, 2.3 g/100 seed), was achieved at 480 min; longer DT did not increase oil yields. The concentrations of the …

The Human Gut Mycobiome: Pitfalls And Potentials — A Mycologist's Perspective, Mallory J. Suhr, Heather E. Hallen-Adams

Department of Food Science and Technology: Faculty Publications

We have entered the Age of the Microbiome, with new studies appearing constantly and whole journals devoted to the human microbiome. While bacteria outnumber other gut microbes by orders of magnitude, eukaryotes are consistently found in the human gut, and are represented primarily by the fungi. Compiling 36 studies spanning from 1917 to 2015, we found at least 267 distinct fungal taxa have been reported from the human gut, and seemingly every new study includes one or more fungi not previously described from this niche. This diversity, while impressive, is illusory. If we examine gut fungi, we will quickly observe …

Orally Administered Extract From Prunella Vulgaris Attenuates Spontaneous Colitis In Mdr1a-/- Mice, Kelley M.K. Haarberg, Meghan J. Wymore Brand, Anne-Marie C. Overstreet, Catherine C. Hauck, Patricia A. Murphy, Jesse M. Hostetter, Amanda Ramer-Tait, Michael J. Wannemuehler

Department of Food Science and Technology: Faculty Publications

AIM: To investigate the ability of a Prunella vulgaris (P. vulgaris) ethanolic extract to attenuate spontaneous typhlocolitis in mdr1a^-/- mice.

METHODS: Vehicle (5% ethanol) or P. vulgaris ethanolic extract (2.4 mg/d) were administered daily by oral gavage to mdr1a^-/- or wild type FVB^WT mice from 6 wk of age up to 20 wk of age. Clinical signs of disease were noted by monitoring weight loss. Mice experiencing weight loss in excess of 15% were removed from the study. At the time mice were removed from the study, blood and colon tissue were collected for analyses that …

Resistant Starches For The Management Of Metabolic Diseases, Laure B. Bindels, Jens Walter, Amanda E. Ramer-Tait

Department of Food Science and Technology: Faculty Publications

Purpose of review—Recent clinical trials and animal studies indicate that resistant starches (RS) may be beneficial therapeutic tools for the management of metabolic diseases. The purpose of this review is to summarize these findings and discuss the established and proposed mechanisms by which RS exert their benefits. We also examine open questions regarding how RS improve metabolism and propose future research directions for the field.

Recent findings—Data from both humans and animal models clearly support a role for RS in improving a variety of metabolic features; however, discrepancies do exist regarding specific effects. Concomitant improvements in both insulin …

Probiotics And Methods Of Obtaining Same, Jens Walter, Bob Thomas, Thomas Burkey

Department of Food Science and Technology: Faculty Publications

This disclosure describes novel probiotics, and also describes novel methods by which such probiotics can be obtained.

Correlation Of Periodontal Pathogens In Concurrent Endodontic-Periodontal Diseases, Amela Lačević, Federico Foschi, Lejla Pojskić, Naris Pojskić, Kasim Bajrović, Jacques Izard

Department of Food Science and Technology: Faculty Publications

Objectives: This study investigated the correlation between Tannerella forsythia, Porphyromonas gingivalis, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans at dual sites in concurrent endodonticperiodontal diseases.

Material and methods: Samples were collected from endodontium and periodontium in cases of concurrent endodontic-periodontal diseases from thirty participants. The sensitivity and specificity of SYBR Green real-time PCR was used to identify the targeted species. Absolute number of targeted genome copies in tested samples were extrapolated from respective calibration curve.

Results: No statistical difference was found in the number of detected endodontic-periodontal pathogens between the endodontium and periodontium. The Pearson test …

Ability Of The Gut Microbiota To Produce Pufa-Derived Bacterial Metabolites: Proof Of Concept In Germ-Free Versus Conventionalized Mice, Céline Druart, Laure B. Bindels, Robert Schmaltz, Audrey M. Neyrinck, Patrice D. Cani, Jens Walter, Amanda E. Ramer-Tait, Nathalie M. Delzenne

Department of Food Science and Technology: Faculty Publications

Scope—The gut microbiota is able to modulate host physiology through the production of bioactive metabolites. Our recent studies suggest that changes in gut microbiota composition upon prebiotics supplementation alter tissue levels of PUFA-derived metabolites in mice. However, in vivo evidence that gut microbes produces PUFA-derived metabolites is lacking. This study aimed to decipher the contribution of gut microbes versus that of the host in PUFA-derived metabolite production.

Methods and results—To achieve this goal, we compared the proportion of PUFA-derived metabolites and the expression of fatty acid desaturases in germ-free (GF) and conventionalized (CONV) mice fed either a low …

A Prototype On-Line Aotf Hyperspectral Image Acquisition System For Tenderness Assessment Of Beef Carcasses, Govindarajan Konda Naganathan, Kim Cluff, Ashok Samal, Chris Calkins, David D. Jones, Carol Lorenzen, Jeyamkondan Subbiah

Department of Food Science and Technology: Faculty Publications

A prototype on-line acousto-optic tunable filter (AOTF)-based hyperspectral image acquisition system (λ = 450– 900 nm) was developed for tenderness assessment of beef carcasses. Hyperspectral images of ribeye muscle on stationary hanging beef carcasses (n = 338) at 2-day postmortem were acquired in commercial beef slaughter or packing plants. After image acquisition, a strip steak was cut from each carcass, vacuum packaged, aged for 14 days, cooked, and slice shear force tenderness scores were collected by an independent lab. Beef hyperspectral images were mosaicked together and principal component (PC) analysis was conducted to reduce the spectral dimension. Six different textural …

Fungi Inhabiting The Healthy Human Gastrointestinal Tract: A Diverse And Dynamic Community, Heather E. Hallen-Adams, Stephen D. Kachman, Jaehyoung Kim, Ryan Legge, Inés Martínez

Department of Food Science and Technology: Faculty Publications

Fungal DNA was selectively amplified, and the ITS region sequenced, from fecal samples taken from 45 healthy human volunteers at one (21 volunteers) or two (24 volunteers) time points. Seventy-two operational taxonomic units, representing two phyla and ten classes of fungi, were recovered. Candida yeasts, notably C. tropicalis (present in 51 samples), and yeasts in the Dipodascaceae (39 samples), dominated, while 38 OTUs were detected in a single sample each. Fungi included known human symbionts (Candida, Cryptococcus, Malassezia and Trichosporon spp.), common airborne fungi (Cladosporium sp.) and fungi known to be associated with food ( …

The Gut Microbiota Of Rural Papua New Guineans: Composition, Diversity Patterns, And Ecological Processes, Ines Martinez, James C. Stegen, Maria X. Maldonado-Gomez, A. Murat Eren, Peter M. Siba, Andrew R. Greenhill, Jens Walter

Department of Food Science and Technology: Faculty Publications

Although recent research revealed an impact of westernization on diversity and composition of the human gut microbiota, the exact consequences on metacommunity characteristics are insufficiently understood, and the underlying ecological mechanisms have not been elucidated. Here, we have compared the fecal microbiota of adults from two non-industrialized regions in Papua New Guinea (PNG) with that of United States (US) residents. Papua New Guineans harbor communities with greater bacterial diversity, lower inter-individual variation, vastly different abundance profiles, and bacterial lineages undetectable in US residents. A quantification of the ecological processes that govern community assembly identified bacterial dispersal as the dominant process …

Diversity Of Yeast And Mold Species From A Variety Of Cheese Types, Nabaraj Banjara, Mallory J. Suhr, Heather E. Hallen-Adams

Department of Food Science and Technology: Faculty Publications

To generate a comprehensive profile of viable fungi (yeasts and molds) on cheese as it is purchased by consumers, 44 types of cheese were obtained from a local grocery store from 1 to 4 times each (depending on availability) and sampled. Pure cultures were obtained and identified by DNA sequence of the ITS region, as well as growth characteristics and colony morphology. The yeast Debaryomyces hansenii was the most abundant fungus, present in 79 % of all cheeses and 63 % of all samples. Penicillium roqueforti was the most common mold, isolated from a variety of cheeses in addition to …

Food Fish Identification From Dna Extraction Through Sequence Analysis, Heather E. Hallen-Adams

Department of Food Science and Technology: Faculty Publications

This experiment exposed third and fourth year undergraduates and graduate students taking a course in advanced food analysis to DNA extraction, polymerase chain reaction (PCR), and DNA sequence analysis. Students provided their own fish sample, purchased from local grocery stores, and the class as a whole extracted DNA, which was then subjected to PCR, gel electrophoresis and Sanger sequencing. Students retrieved their sequences and identified their fish using the NCBI BLAST nucleotide database. Slightly more than half of the samples yielded sequences identical or close to expected (based on the identification of the fish on the packaging); some other samples …

Sourdough Lactic Acid Bacteria As Antifungal And Mycotoxin-Controlling Agents, Yousef I. Hassan, Ting Zhou, Lloyd B. Bullerman

Department of Food Science and Technology: Faculty Publications

Sourdough starter cultures are rich sources of endogenous lactic acid bacteria. The extended shelf lives of sourdough breads are attributed to a large array of organic acids and low-molecular-weight metabolites produced during the fermentation process. Different species belonging to the lactic acid bacteria group of microorganisms, mainly Lactobacillus and Leuconostoc, are increasingly gaining the attention as possible means for inhibiting mold growth in animal feed and human food chains. In addition, certain lactic acid bacteria strains isolated from sourdough starters were also shown to reduce mycotoxins concentrations in contaminated products either by binding or degradation. This short review will …

Processing Effects On Four Prebiotic Carbohydrates Supplemented In An Extruded Cereal And A Low Ph Drink, Rebbeca M. Duar, Pei Tze Ang, Michelle Hoffman, Randy Wehling, Robert W. Hutkins, Vicki Schlegel

Department of Food Science and Technology: Faculty Publications

Prebiotic carbohydrates are added as functional ingredients to a variety of processed foods. Data on the stability of prebiotics during food processing in complex matrices remain limited. The objective of this project was to determine the stability of fructooligosaccharides (FOS), inulin, galactooligosaccharides (GOS), and resistant starch (RS₂), when added as ingredients (1% w/w) to an extruded cereal and a low pH drink. The cereal was prepared using different screw speeds and barrel temperatures. GOS was not affected by any of the extrusion conditions, whereas inulin decreased significantly at 140 and 170°C. FOS levels decreased in all extrusion conditions, …

Alignment Behaviors Of Short Peptides Provide A Roadmap For Functional Profiling Of Metagenomic Data, Rohita Sinha, Jennifer L. Clarke, Andrew K. Benson

Department of Food Science and Technology: Faculty Publications

Background: Functional assignments for short-read metagenomic data pose a significant computational challenge due to perceived unpredictability of alignment behavior and the inability to infer useful functional information from translated protein-fragments/peptides. To address this problem, we have examined the predictability of short peptide alignments by systematically studying alignment behavior of large sets of short peptides generated from well-characterized proteins as well as hypothetical proteins in the KEGG database.

Results: Using test sets of peptides modeling the length and phylogenetic distributions of short-read metagenomic data, we observed that peptides from well-characterized proteins had indistinguishable alignments to proteins from the same orthologous family …

Host Genetic Architecture And The Landscape Of Microbiome Composition: Humans Weigh In, Andrew K. Benson

Department of Food Science and Technology: Faculty Publications

Comparative analyses of the control of mammalian microbiomes by host genetic architecture reveal striking conserved features that have implications for the evolution of host–microbiome interactions.

Quantitative Real-Time Polymerase Chain Reaction For Detecting Mycoplasma Hyosynoviae And Mycoplasma Hyorhinis In Pen-Based Oral, Tonsillar, And Nasal Fluids, João Carlos Gomes Neto, Leslie Bower, Barbara Z. Erickson, Chong Wang, Matthew Raymond, Erin L. Strait

Department of Food Science and Technology: Faculty Publications

Mycoplasma (M.) hyorhinis and M. hyosynoviae are pathogens known to cause disease in pigs post-weaning. Due to their fastidious nature, there is increased need for culture-independent diagnostic platforms to detect these microorganisms. Therefore, this study was performed to develop and optimize quantitative real-time PCR (qPCR) assays to rapidly detect M. hyorhinis and M. hyosynoviae in pen-based oral fluids as well as nasal and tonsillar fluids as proxies for samples used in swine herd surveillance. Two methods of genomic DNA extraction, automated versus manual, were used to compare diagnostic test performance. A wean-to-finish longitudinal study was also carried out to demonstrate …