Food Science Commons^™

Drainage Ditch Research Reveals Opportunities For Cleaning Up Runoff, Ann Perry

Agricultural Research Magazine

There is no typical drainage ditch,” says Agricultural Research Service ecologist Matt Moore. “And until recently, farmers just thought of them as something they need to drain water off their fields. But we can use these ditches to minimize pesticide and nutrient losses in runoff—and it can be done without taking any cropland out of production.”

Moore, who works at the ARS National Sedimentation Laboratory in Oxford, Mississippi, has been wading through edge of- field drainage ditches since he was a boy planting rice on his family’s farm in Arkansas. The ditches—as common in agricultural landscapes as the fields they …

Going Beyond The Barnyard To Stop Stable Flies, David B. Taylor, J.J. Zhu, Sandra Avant

Agricultural Research Magazine

Livestock producers may not be able to see the difference between stable flies and other flies at a distance, but they can definitely see the stable flies’ effect on their cattle as the animals stop grazing and bunch together to minimize the number of bites they’re getting.

Stable flies are among the most important arthropod pests of cattle in the United States. Their painful bites can reduce milk production in dairy cows, decrease weight gain in beef cattle, and reduce feed efficiency.

Generally, insecticide sprays are used to help keep stable flies off animals, especially their legs, where the flies …

Citrus Greening: A Puzzle With Many Pieces, Gail Wisler

Agricultural Research Magazine

Finding the solution to some crop diseases is like solving a jigsaw puzzle. The solution has many pieces, and fitting them together can be a challenge. Citrus greening, also known as “Huanglongbing” (HLB), is just such a disease. HLB is costing Florida citrus growers millions of dollars each year in lost revenue from unproductive trees. It continues to spread and is the most serious threat to the U.S. citrus industry in history. The disease was recently confirmed in commercial groves in Texas and in a homeowner’s yard in California. Both states are important citrus producers, and if HLB spreads in …

Helping Citrus Growers Deal With A Nasty Invader, Dennis O’Brien

Agricultural Research Magazine

Growers battling a devastating citrus disease may soon benefit from a one-two punch from Florida researchers. Two Agricultural Research Service scientists at the U.S. Horticultural Research Laboratory in Fort Pierce are using two very different strategies to help citrus growers and processors deal with Huanglongbing (HLB), a disease that poses a major threat to the survival of the citrus industry and is costing it millions of dollars each year.

Elizabeth Baldwin, research leader of the Citrus and Subtropical Products Research Unit, is leading a team developing technology that orange juice processors can use to determine whether their product has the …

New Technology For Harvesting The Power Of Beneficial Fungi, Mark Jackson, Jan Suszkiw

Agricultural Research Magazine

Biopesticides containing beneficial fungi are often grown on grains or other solids, but Agricultural Research Service scientists have found that a liquid diet might be cheaper and better. The approach, known as “liquid culture fermentation,” offers several advantages, including lower material costs and increased yields of certain forms of pestkilling fungi like Isaria or Metarhizium that can be sprayed directly onto crop plants or applied to soil as a biological alternative to using synthetic pesticides.

For decades, biopesticide makers have cultured fungi like these on moistened grains or other solid substrates to prompt them into churning out billions of specialized …

January 2013- Locations Featured In This Magazine Issue

Agricultural Research Magazine

The Agricultural Research Service has about 100 labs all over the country.

Locations Featured in This Magazine Issue

Plant Gene Expression Center, Albany, California 1 research unit ■ 12 employees

Corvallis, Oregon 3 research units ■ 133 employees

Davis, California 3 research units ■ 114 employees

San Joaquin Valley Agricultural Sciences Center, Parlier, California 3 research units ■ 125 employees

U.S. Salinity Laboratory, Riverside, California 2 research units ■ 36 employees

Pullman, Washington 6 research units 136 employees

Fort Collins, Colorado 7 research units ■ 141 employees

Northern Plains Agricultural Research Laboratory, Sidney, Montana 2 research units ■ 72 employees …

Orange Juice Amino Acids May Reveal Secrets To Quelling Citrus Greening Disease, Andrew P. Breksa Iii, Marcia Wood

Agricultural Research Magazine

Amino acids in orange juice may hold clues to the mostly secret, highly successful attack strategy of a powerful plant pathogen. Recent studies of these compounds may pave the way to a safe, effective, environmentally friendly way to vanquish this orchard enemy.

Known as Candidatus Liberibacter asiaticus, this microbe causes citrus greening disease, also called “Huanglongbing,” or “HLB.” (See article on page 4).

Agricultural Research Service chemist Andrew P. Breksa III and University of California-Davis professor Carolyn M. Slupsky have compared the amino acid composition of juice from commercially grown oranges. They used nuclear magnetic resonance spectroscopy to study juice …

Agricultural Research Magazine, January 2013

Agricultural Research Magazine

Table of Contents

4 Helping Citrus Growers Deal With a Nasty Invader

7 Orange Juice Amino Acids May Reveal Secrets to Quelling Citrus Greening Disease

8 Drainage Ditch Research Reveals Opportunities for Cleaning Up Runoff

11 Crop-Friendly Bacteria Tapped To Battle Fungal Marauders

12 Advantages of Understanding the Lady Beetle Diet

13 New Uses for Sugar Beet Pulp

14 Going Beyond the Barnyard To Stop Stable Flies

16 The Real Dirt on Biosolids as Soil Amendments

17 With Hot Air Treatment, Bacteria Fly the Coop

18 ARS Works Toward Control of Brown Marmorated Stink Bug

21 New Technology for Harvesting …

New Uses For Sugar Beet Pulp, Linshu Liu, Rosalie Marion Bliss

Agricultural Research Magazine

More than 1 million tons of sugar beet pulp are generated annually by U.S. beet sugar industries. Finding profitable uses for the biodegradable pulp, which is the leftover residue from sugar extraction, is critical for the long-term economic viability of U.S. agribusiness.

Agricultural Research Service researchers and colleagues have long been studying the potential of sugar beet pulp utilization. Now, chemist LinShu Liu and plant physiologist Arland Hotchkiss, both with the Dairy and Functional Foods Unit at ARS’s Eastern Regional Research Center in Wyndmoor, Pennsylvania, and colleagues have found new uses for sugar beet pulp.

In collaboration with professor Jinwen …

Ars Works Toward Control Of Brown Marmorated Stink Bug, Sharon Durham, Dennis O’Brien

Agricultural Research Magazine

The brown marmorated stink bug (BMSB) is wreaking havoc in U.S. homes, gardens, and agricultural operations, causing personal and economic woe. Agricultural Research Service scientists are exploring various aspects of monitoring and control of this increasingly important insect pest, which is an invasive Asian species known as a sporadic pest of many tree fruit crops in China, Korea, and Japan. Along with being a household nuisance, it is a major economic threat to producers of orchard fruits such as apple, peach, and pear; garden vegetables and row crops; and many ornamental species.

Since its detection in the northeastern United States …

Guia Tecnica Para La Produccion Artesanal De Semilla De Variedades De Polinizacion Libre De Sorgo En America Central, René Clará Valencia

INTSORMIL Scientific Publications

La producción de semilla artesanal de sorgo es un complemento en la producción y uso de semilla certificada y tiene como objetivo el de atender a los agricultores que por diversas razones no acceden a la semilla certificada, pero son sujetos potenciales de producción y desarrollo del cultivo de sorgo. En términos generales son los agricultores de menos recursos, que representan la mayoría de productores de sorgo en América Central, los que se ven limitados a la utilización de las variedades mejoradas. Debemos de meditar sobre la utilización de las variedades mejoradas de polinización libre y probablemente el poco acceso …

Crop-Friendly Bacteria Tapped To Battle Fungal Marauders, Patricia Okubara, Jan Suszkiw

Agricultural Research Magazine

Soil-dwelling bacteria that depend on wheat and barley roots for their “room and board” could soon make good on their debt. Researchers are investigating the microbes’ potential to biologically control root-rot fungi that cause crop yield losses of 10-30 percent annually in the U.S. Pacific Northwest and other parts of the world.

The bacteria are members of the genus Pseudomonas and include 11 strains that stymie the growth of Pythium and Rhizoctonia fungi, which are responsible for dampingoff and root-rot diseases of wheat and barley. The pathogens thrive in cool, moist soils and can reach especially high levels in crop …

Characterization Of Putative Ige-Reactive N-Terminal Peptides From The 7s Vicilin-Like Globulin Of English Walnut (Juglans Regia), M Downs, J. Baumert, S Taylor, E. N. C. Mills

Department of Food Science and Technology: Faculty Publications

Background The 7S vicilin-like seed storage globulins represent an important class of food allergens. In many plants, the 7S globulins are synthesized as a precursor polypeptide, which undergoes post-translational proteolysis to remove a portion of the N-terminus to produce the mature protein sequence. However, the fate of the cleaved N-terminal region is largely unknown. We have identified and characterized putative IgE-binding peptides derived from the 7S vicilin N-terminus in English walnut (Juglans regia).

Methods The low-molecular weight (LMW) protein fraction from raw, defatted English walnuts (cv. Chandler) was purified using Con-A sepharose, gel filtration, and anion exchange chromatography. …

Diet-Induced Alterations Of Host Cholesterol Metabolism Are Likely To Affect The Gut Microbiota Composition In Hamsters, Inés Martínez, Diahann J. Perdicaro, Andrew W. Brown, Susan Hammons, Trevor J. Carden, Timothy P. Carr, Kent M. Eskridge, Jens Walter

Department of Food Science and Technology: Faculty Publications

The gastrointestinal microbiota affects the metabolism of the mammalian host and has consequences for health. However, the complexity of gut microbial communities and host metabolic pathways make functional connections difficult to unravel, especially in terms of causation. In this study, we have characterized the fecal microbiota of hamsters whose cholesterol metabolism was extensively modulated by the dietary addition of plant sterol esters (PSE). PSE intake induced dramatic shifts in the fecal microbiota, reducing several bacterial taxa within the families Coriobacteriaceae and Erysipelotrichaceae. The abundance of these taxa displayed remarkably high correlations with host cholesterol metabolites. Most importantly, the associations …

The Protein Structure Determines The Sensitizing Capacity Of Brazil Nut 2s Albumin (Ber E1) In A Rat Food Allergy Model, Jolanda Hm Van Bilsen, Léon Mj Knippels, André H. Penninks, Willem F. Nieuwenhuizen, Harmen Hj De Jongh, Stef J. Koppelman

Department of Food Science and Technology: Faculty Publications

It is not exactly known why certain food proteins are more likely to sensitize. One of the characteristics of most food allergens is that they are stable to the acidic and proteolytic conditions in the digestive tract. This property is thought to be a risk factor in allergic sensitization. The purpose of the present study was to investigate the contribution of the protein structure of 2S albumin (Ber e1), a major allergen from Brazil nut, on the sensitizing capacity in vivo using an oral Brown Norway rat food allergy model. Disulphide bridges of 2S albumin were reduced and …

Allergenicity Assessment Of A Genetically Modified Protein-Recombinant Human Lactoferrin, Cui Zhou, Na Sun, Jing Wang, Jing Lu, Jing Tian, Richard E. Goodman, Ning Li, Huilian Che, Kunlun Huang

Department of Food Science and Technology: Faculty Publications

Background: Recombinant human lactoferrin (rhLF) has previously suggested serving as food additive due to the natural iron binding properties that provide anti-microbial activity. Recombinant cows have been produced that express hLf in milk. However, the potential allergenicity of hLf has not been previously assessed. This research is conducted to evaluate the potential allergenicity of rhLF as a prerequisite for food use.

Methods: A comparison was made of the bioactivity, physicochemical properties and glycosylation profile between rhLF and natural hLF. The amino acid sequence of hLf was compared to known allergens. Additionally, the stability of hLf in pepsin and a human …

Evaluation Of Endogenous Allergens For The Safety Evaluation Of Genetically Engineered Food Crops: Review Of Potential Risks, Test Methods, Examples And Relevance, Richard E. Goodman, Rakhi Panda, Harsha Ariyarathna

Department of Food Science and Technology: Faculty Publications

The safety of food produced from genetically engineered (GE) crops is assessed for potential risks of food allergy on the basis of an international consensus guideline outlined by the Codex Alimentarius Commission (2003). The assessment focuses on evaluation of the potential allergenicity of the newly expressed protein(s) as the primary potential risk using a process that markedly limits risks to allergic consumers. However, Codex also recommended evaluating a second concern, potential increases in endogenous allergens of commonly allergenic food crops that might occur due to insertion of the gene. Unfortunately, potential risks and natural variation of endogenous allergens in non-GE …

The Protein Structure Determines The Sensitizing Capacity Of Brazil Nut 2s Albumin (Ber E1) In A Rat Food Allergy Model, Jolanda H. M. Van Bilsen, Leon M.J. Knippels, Andre H. Penninks, Willem F. Nieuwenhuizen, Harmen H.J. De Jongh, Stef J. Koppelman

Department of Food Science and Technology: Faculty Publications

It is not exactly known why certain food proteins are more likely to sensitize. One of the characteristics of most food allergens is that they are stable to the acidic and proteolytic conditions in the digestive tract. This property is thought to be a risk factor in allergic sensitization. The purpose of the present study was to investigate the contribution of the protein structure of 2S albumin (Ber e1), a major allergen from Brazil nut, on the sensitizing capacity in vivo using an oral Brown Norway rat food allergy model. Disulphide bridges of 2S albumin were reduced and …

Digestibility And Ige-Binding Of Glycosylated Codfish Parvalbumin, Harmen H. J. De Jongh, Carlos López Robles, Eefjan Timmerman, Julie A. Nordlee, Poi-Wah Lee, Joseph L. Baumert, Robert G. Hamilton, Steve L. Taylor, Stef J. Koppelman

Department of Food Science and Technology: Faculty Publications

Food-processing conditions may alter the allergenicity of food proteins by different means. In this study, the effect of the glycosylation as a result of thermal treatment on the digestibility and IgE-binding of codfish parvalbumin is investigated. Native and glycosylated parvalbumins were digested with pepsin at various conditions relevant for the gastrointestinal tract. Intact proteins and peptides were analysed for apparent molecular weight and IgE-binding. Glycosylation did not substantially affect the digestion. Although the peptides resulting from digestion were relatively large (3 and 4 kDa), the IgE-binding was strongly diminished. However, the glycosylated parvalbumin had a strong propensity to form dimers …

A Systematic Review Of The Effect Of Thermal Processing On The Allergenicity Of Tree Nuts, L. J. Masthoff, R. Hoff, K. C. M. Verhoeckx, H. Van Os-Medendorp, A. Michelsen-Huisman, Joseph L. Baumert, S. G. Pasmans, Y. Meijer, A. C. Knulst

Department of Food Science and Technology: Faculty Publications

Background: Allergenicity of foods can be influenced by processing. Tree nuts are an important source of nutrition and increasingly consumed; however, processing methods are quite variable and data are currently lacking on the effects of processing on allergenicity.

Objective: To perform a systematic literature review on the effects of food processing on the allergenicity of tree nuts.

Methods: A systematic literature search of PubMed and Embase databases was performed, with screening of references, related articles and citations. Studies were included if they assessed the allergenicity or immunogenicity of processed nuts.

Results: The search resulted in 32 articles suitable for …

Combinatorial Evaluation Of In Vivo Distribution Of Polyanhydride Particle-Based Platforms For Vaccine Delivery, Latrisha K. Petersen, Lucas M. Huntimer, Katharine Walz, Amanda E. Ramer-Tait, Michael Wannemueler, Balaji Narasimhan

Department of Food Science and Technology: Faculty Publications

Several challenges are associated with current vaccine strategies, including repeated immunizations, poor patient compliance, and limited approved routes for delivery, which may hinder induction of protective immunity. Thus, there is a need for new vaccine adjuvants capable of multi-route administration and prolonged antigen release at the site of administration by providing a depot within tissue. In this work, we designed a combinatorial platform to investigate the in vivo distribution, depot effect, and localized persistence of polyanhydride nanoparticles as a function of nanoparticle chemistry and administration route. Our observations indicated that the route of administration differentially affected tissue residence times. All …

Pinto Beans (Phaseolus Vulgaris L.) As A Functional Food: Implications On Human Health, Cristiane R. S. Câmara, Carlos A. Urrea, Vicki Schlegel

Department of Food Science and Technology: Faculty Publications

Most foods are considered functional in terms of providing nutrients and energy to sustain daily life, but dietary systems that are capable of preventing or remediating a stressed or diseased state are classified as functional foods. Dry beans (Phaseolus vulgaris L.) contain high levels of chemically diverse components (phenols, resistance starch, vitamins, fructooligosaccharides) that have shown to protect against such conditions as oxidative stress, cardiovascular disease, diabetes, metabolic syndrome, and many types of cancer, thereby positioning this legume as an excellent functional food. Moreover, the United States has a rich dry bean history and is currently a top producer …

Neisseria Oralis Sp. Nov., Isolated From Healthy Gingival Plaque And Clinical Samples, William J. Wolfgang, Teresa V. Passaretti, Reashma Jose, Jocelyn Cole, An Coorevits, Andrea N. Carpenter, Sherly Jose, Anita Van Landschoot, Jacques Izard, Donna J. Kohlerschmidt, Peter Vandamme, Floyd E. Dewhirst, Mark A. Fisher, Kimberlee A. Musser

Department of Food Science and Technology: Faculty Publications

A polyphasic analysis was undertaken of seven independent isolates of gram-negative cocci collected from pathological clinical samples from New York, Louisiana, Florida, and Illinois and healthy subgingival plaque from a patient in Virginia, USA. The 16S rRNA gene sequence similarity among these isolates was 99.7–100 %, and the closest species with a validly published name was Neisseria lactamica (96.9 % similarity to the type strain). DNA–DNA hybridization confirmed that these isolates are of the same species and are distinct from their nearest phylogenetic neighbor, N. lactamica. Phylogenetic analysis of 16S and 23S rRNA gene sequences indicated that the …

Yield Gap Analysis With Local To Global Relevance—A Review, Martin K. Van Ittersum, Kenneth Cassman, Patricio Grassini, Joost Wolf, Pablo Tittonell, Zvi Hochman

Department of Agronomy and Horticulture: Faculty Publications

Yields of crops must increase substantially over the coming decades to keep pace with global food demand driven by population and income growth. Ultimately global food production capacity will be limited by the amount of land and water resources available and suitable for crop production, and by biophysical limits on crop growth. Quantifying food production capacity on every hectare of current farmland in a consistent and transparent manner is needed to inform decisions on policy, research, development and investment that aim to affect future crop yield and land use, and to inform on-ground action by local farmers through their knowledge …

Severe Reactions To A Soy Containing Beverage In Peanut Allergic Individuals Not Avoiding Soy, Benjamin Remington, Julie A. Nordlee, John R. Bacon, Marc E. Dyer, Steve L. Taylor, Joe L. Baumert

Department of Food Science and Technology: Faculty Publications

Rationale: Three peanut-allergic individuals experienced severe reactions to a nationally distributed high-protein beverage in the same month. The major protein sources in the beverage were soy-based ingredients and cows’ milk. These individuals did not avoid soy in their diet and previously consumed various soy products without adverse reactions. Peanut contamination of the beverage was not detected by specific ELISA, so attention shifted to the soy ingredients. The research objective was to elucidate differences between the soy milk and soy protein isolate used in the beverage and other forms of soy safely consumed by these individuals.

Methods: Specific IgE levels from …

Molecular Characterization Of Host-Specific Biofilm Formation In A Vertebrate Gut Symbiont, Steven A. Frese, Donald A. Mackenzie, Daniel A. Peterson, Robert Schmaltz, Teresa Fangman, You Zhou, Chaomei Zhang, Andrew K. Benson, Liz A. Cody, Francis Mulholland, Nathalie Juge, Jens Walter

Department of Food Science and Technology: Faculty Publications

Although vertebrates harbor bacterial communities in their gastrointestinal tract whose composition is host-specific, little is known about the mechanisms by which bacterial lineages become selected. The goal of this study was to characterize the ecological processes that mediate host-specificity of the vertebrate gut symbiont Lactobacillus reuteri, and to systematically identify the bacterial factors that are involved. Experiments with monoassociated mice revealed that the ability of L. reuteri to form epithelial biofilms in the mouse forestomach is strictly dependent on the strain’s host origin. To unravel the molecular basis for this host-specific biofilm formation, we applied a combination of transcriptome …

Characterization Of Starch By Vibrational Spectroscopy, Brandon H. Holder

Department of Food Science and Technology: Dissertations, Theses, and Student Research

To develop a dispersive Raman spectroscopic method for measuring amylose-amylopectin ratios of corn starch mixtures, 67 mixtures were prepared by randomly mixing waxy and normal corn starches. Amylose contents were measured using a dual wavelength iodine binding colorimetric method. Raman data were collected from 250 to 3200 cm^-1 using optimized instrument parameters. Partial least-squares (PLS) and principal components regression (PCR) were used to prepare multivariate calibration models; however, PLS commonly outperformed PCR. Truncating the spectra to 250 to 2000 cm^-1 improved the results (r² of validation = 0.831, SEP = 2.90%). Removal of a cold water swelling …

Differential Surface Deposition Of Complement Proteins On Logarithmic And Stationary Phase Leishmania Chagasi Promastigotes, Amanda Ramer-Tait, Soi Meng Lei, Bryan H. Bellaire, Jeffrey K. Beetham

Department of Food Science and Technology: Faculty Publications

Previous works demonstrated that various species of Leishmania promastigotes exhibit differential sensitivity to complement-mediated lysis (CML) during development. Upon exposure to normal human serum (NHS), cultures of Leishmania chagasi promastigotes recently isolated from infected hamsters (fewer than 5 in vitro passages) are CML-sensitive when in the logarithmic growth phase but become CML-resistant upon transition to the stationary culture phase. Visualization by light and electron microscopy revealed dramatic morphological differences between promastigotes from the 2 culture phases following exposure to NHS. Flow cytometric analysis demonstrated that surface deposition of the complement components C3, C5, and C9 correlated inversely with promastigote CML-resistance. …

New Technologies For Whole Wheat Processing: Addressing Milling And Storage Issues, Andres F. Doblado-Maldonado

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Whole wheat flour production and demand has increased dramatically during the last decade due to evidence supporting the benefits of whole grains in the diet. Hence, the food industry has provided a wide variety of new whole grain products. There are unique challenges that accompany whole wheat flour production, especially related to milling and storage. The present thesis provides new strategies on the adaptation of new technologies to overcome whole wheat processing issues. These issues are first discussed in a literature review and then followed by three research studies. In the first study, retail whole wheat flours were evaluated for …

Evidence Of Transmission Of Escherichia Coli O157:H7 To The Tissues Or Phyllo-Plane Of Wheat, From Contaminated Soil, Seeds Or Water, Bismarck Antonio Martinez

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Escherichia coli O157:H7 is a human pathogen that can cause a wide spectrum of disease symptoms, such as bloody diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS). Escherichia coli O157:H7 illness are mainly associated with undercooked beef; however, in recent years outbreaks have been linked to fresh produce such as spinach, lettuce, and sprouts. In 2009, flour was implicated as a contamination source in the consumption of raw cookie dough resulting in 77 illness-cases. The objective of this research was to determine the possible route of transmission of E. coli O157:H7 into the phyllo-plane of wheat using contaminated seed, soil …