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Full-Text Articles in Food Science
Development Of Bacteriophage Based Detection Technique For Food Safety And Environment Monitoring, Ziyuan Wang
Development Of Bacteriophage Based Detection Technique For Food Safety And Environment Monitoring, Ziyuan Wang
Doctoral Dissertations
Foodborne or waterborne pathogens which are responsible for numerous worldwide outbreaks of disease have caused serious health problems and enormous economic cost. Development of new bacterial detection technologies with high sensitivity and specificity is of great importance for food safety and public health. This has highlighted the significance of developing rapid and effective techniques for pathogen screening. Thus we aim to explore a bacteriophage mediated detection system for foodborne or waterborne bacteria monitoring. Bacteriophage as a novel recognition probe has been applied in various biosensor by immobilizing phage particles on solid sensor surface. They possess high specificity for targeted microorganism …
Bacteriophage-Based Colorimetric Detection Of Escherichia Coli In Drinking Water, Juhong Chen
Bacteriophage-Based Colorimetric Detection Of Escherichia Coli In Drinking Water, Juhong Chen
Doctoral Dissertations
One of the major safety causes in drinking water is from the bacteria contamination, especially in developing countries and resource-limited settings. Although many of these Escherichia coli (E. coli) strains in drinking water are nonpathogenic, they sever as the indicator for bacterial contamination. And, the more widely used method to detect E. coli in drinking water is to determine the activity of β-galactosidase (β-gal), which is released by E. coli. Rapid, sensitive and inexpensive detection of E. coli in drinking water can reduce the risk of food-borne bacteria infection and stop the disease widely spreading. In this …
Advanced Materials For Rapid Diagnostics In Food, Agriculture And Healthcare, Charmaine K.W. Koo
Advanced Materials For Rapid Diagnostics In Food, Agriculture And Healthcare, Charmaine K.W. Koo
Doctoral Dissertations
Paper based devices are an emerging trend for micro total analysis systems due to their rapid, sensitive and specific attributes. Lateral flow assays (LFAs), the predecessor of paper-fluidic devices, are developed for many applications which range from nucleic acid and antibody detection to commercial home pregnancy tests. With growing interest in replacing conventional detection methods, multistep assays are needed. These assays require multiple test reagents, therefore, there is a need to control fluid flow for the development of complex paper-based devices. We were able fabricate paper-fluidic platforms where we used electrowetting-on-dielectrics to create valves on paper. With this method, we …
Bacteriophage: Bioengineered Bacterial Detection And Applications, Samuel D. Alcaine
Bacteriophage: Bioengineered Bacterial Detection And Applications, Samuel D. Alcaine
Doctoral Dissertations
Bacteria are ubiquitous and vital constituents of our environment, our foods, and our bodies. A small percentage of this vast, microbial population is pathogenic to humans, but represents a significant burden on public health. There is a current public health focus on two subgroups: foodborne pathogenic bacteria and antibiotic resistance bacteria. A key challenge for public health is the rapid identification of these bacteria to prevent their consumption and to ensure proper treatment for infections. This challenge calls for the development of novel, low-cost diagnostics that combine sensitivity and accuracy with speed and ease-of-use. Bacteriophages represent rapid, readily targeted, and …
Application Of Bacteriophage Cocktail In Leafy Green Wash Water To Control Salmonella Enterica, Andrea W. Lo
Application Of Bacteriophage Cocktail In Leafy Green Wash Water To Control Salmonella Enterica, Andrea W. Lo
Masters Theses
Produce is responsible for 46% of all foodborne illnesses in the USA. Salmonella enterica causes 19,000 hospitalizations each year, and has been associated with produce. Presently, chlorine based sanitizers are most often used, however organic matter reduces its antimicrobial activity. Bacteriophage treatments are an all-natural, alternative method for pathogen inactivation. The objective of this study was to determine the efficacy of a five-strain bacteriophage treatment against a S. enterica cocktail in simulated wash waters at different temperatures. Bacteriophage and S. enterica were enumerated in simulated wash water solutions. One set of experiments studied bacteriophage and S. enterica growth in TSB+vegetable …