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Food Science Commons

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Food Microbiology

Core for Applied Genomics and Ecology (CAGE)

2008

Articles 1 - 5 of 5

Full-Text Articles in Food Science

454 Applications: Just How Creative Can You Be? Sep 2008

454 Applications: Just How Creative Can You Be?

Core for Applied Genomics and Ecology (CAGE)

CONTENTS:
Deep Sequencing and Variant analysis
Microbial Population Structures in the Deep Marine Biosphere
Direct Selection of Human Genomic Loci by Microarray Hybridization
Whole Genome Sequencing and Resequencing
Transcriptome mapping
Specialized methods for cDNA sequencing
Library Normalization by dsDNA nuclease digestion


Next Generation Dna Sequencing And The Birth Of A Golden Era For Microbial Ecology Sep 2008

Next Generation Dna Sequencing And The Birth Of A Golden Era For Microbial Ecology

Core for Applied Genomics and Ecology (CAGE)

In 2005, a breakthrough technology was Announced that provided a quantum leap In DNA sequencing capacity.

This technology differed from the Sanger method In many ways:
1. Eliminates cloning—completely circumvents cloning bias
2. Uses bead technology—DNA templates are attached to sepharose beads
3. Relies on pyrosequencing chemistry—synthesis is sequential not partial
4. Parallelizes the sequencing process—hundreds of thousands of beads can be sequenced in parallel

The bottom line: it makes production-scale sequencing available to the common man.


Bioinformatics And Data Analysis Sep 2008

Bioinformatics And Data Analysis

Core for Applied Genomics and Ecology (CAGE)

CONTENTS:
Run-time applications
Post-run applications
Image processing
Signal processing
de novo Assembly
Reference Mapper
Variant analysis


Unl Announces Opening Of Core For Applied Genomics And Ecology (Cage), Andy Benson Jan 2008

Unl Announces Opening Of Core For Applied Genomics And Ecology (Cage), Andy Benson

Core for Applied Genomics and Ecology (CAGE)

The UNL Core for Applied Genomics and Ecology (CAGE) announces that it is now offering a new suite of services to UNL investigators and outside entities. Formerly known as the UNL Printed Microarray Core Facility, CAGE now offers Next Gen sequencing on its newly installed Roche-454 GS FLX. In support of the UNL Gut Function Initiative, CAGE specializes in application of the GS-FLX platform for analysis of complex microbial communities by massively parallel sequencing of 16S rRNA amplicons. CAGE also has expertise in shotgun sequencing applications on the instrument, including whole genome sequencing and analysis of BACs and metagenome libraries. …


Next Gen Sequencing: An Alternative To Culture-Based Microbiological Testing Jan 2008

Next Gen Sequencing: An Alternative To Culture-Based Microbiological Testing

Core for Applied Genomics and Ecology (CAGE)

Next Gen DNA Sequencing Enables You...
• to analyze microbial communities and ecosystems in food products
• perform food safety testing
• address spoilage and other non-safety issues
• optimize food processing
• create databases of “good” microbial profiles associated with target product characteristics