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Full-Text Articles in Food Science

North Atlantic Vibrio Vulnificus Surveillance From Postharvest Oysters At A Us Shellfish Processing Facility, Kenneth Lavalley, Joseph Dealteris, Michael Rice, Marta Gomez-Chiarri Dec 2013

North Atlantic Vibrio Vulnificus Surveillance From Postharvest Oysters At A Us Shellfish Processing Facility, Kenneth Lavalley, Joseph Dealteris, Michael Rice, Marta Gomez-Chiarri

Marta Gomez-Chiarri

Postharvest surveillance for Vibrio vulnificus by a commercial processing facility was conducted from May 2001 to September 2003. Harvest areas included the Delaware Bay, Long Island Sound and Prince Edward Island. Occurrence followed a seasonal distribution. Low densities were observed in June, increased through August, and became rare by September. Given the ubiquitous nature of marine Vibrios, it was not surprising to find V. vulnificus everywhere, which was investigated. Observations confirmed the importance of strict time/temperature and product handling practices by shellfish harvesters, dealers and processors. Mishandling led to a potentially dangerous and uncharacteristically high V. vulnificus observation.


Synthesis And Antimicrobial Evaluation Of Carbohydrate And Polyhydroxylated Non-Carbohydrate Fatty Acid Ester And Ether Derivatives, Aoife Smith, Patricia Nobmann, Gary Henehan, Paula Bourke, Julie Dunne Oct 2008

Synthesis And Antimicrobial Evaluation Of Carbohydrate And Polyhydroxylated Non-Carbohydrate Fatty Acid Ester And Ether Derivatives, Aoife Smith, Patricia Nobmann, Gary Henehan, Paula Bourke, Julie Dunne

Articles

A series of fatty acid ester and ether derivatives have been chemically synthesised based on carbohydrate and non-carbohydrate polyhydroxylated scaffolds. The synthesised compounds, along with their corresponding fatty acid monoglyceride antimicrobials, were evaluated for antimicrobial activity against Staphylococcus aureus and Escherichia coli. Of the derivatives synthesised several of the carbohydrate based compounds have antimicrobial efficacy comparable with commercially available antimicrobials. The results suggest that the nature of the carbohydrate core plays a role in the efficacy of carbohydrate fatty acid derivatives as antimicrobials.


454 Applications: Just How Creative Can You Be? Sep 2008

454 Applications: Just How Creative Can You Be?

Core for Applied Genomics and Ecology (CAGE)

CONTENTS:
Deep Sequencing and Variant analysis
Microbial Population Structures in the Deep Marine Biosphere
Direct Selection of Human Genomic Loci by Microarray Hybridization
Whole Genome Sequencing and Resequencing
Transcriptome mapping
Specialized methods for cDNA sequencing
Library Normalization by dsDNA nuclease digestion


Next Generation Dna Sequencing And The Birth Of A Golden Era For Microbial Ecology Sep 2008

Next Generation Dna Sequencing And The Birth Of A Golden Era For Microbial Ecology

Core for Applied Genomics and Ecology (CAGE)

In 2005, a breakthrough technology was Announced that provided a quantum leap In DNA sequencing capacity.

This technology differed from the Sanger method In many ways:
1. Eliminates cloning—completely circumvents cloning bias
2. Uses bead technology—DNA templates are attached to sepharose beads
3. Relies on pyrosequencing chemistry—synthesis is sequential not partial
4. Parallelizes the sequencing process—hundreds of thousands of beads can be sequenced in parallel

The bottom line: it makes production-scale sequencing available to the common man.


Bioinformatics And Data Analysis Sep 2008

Bioinformatics And Data Analysis

Core for Applied Genomics and Ecology (CAGE)

CONTENTS:
Run-time applications
Post-run applications
Image processing
Signal processing
de novo Assembly
Reference Mapper
Variant analysis


North Atlantic Vibrio Vulnificus Surveillance From Postharvest Oysters At A Us Shellfish Processing Facility, Kenneth Lavalley, Joseph Dealteris, Michael Rice, Marta Gomez-Chiarri Jun 2008

North Atlantic Vibrio Vulnificus Surveillance From Postharvest Oysters At A Us Shellfish Processing Facility, Kenneth Lavalley, Joseph Dealteris, Michael Rice, Marta Gomez-Chiarri

Michael A Rice

Postharvest surveillance for Vibrio vulnificus by a commercial processing facility was conducted from May 2001 to September 2003. Harvest areas included the Delaware Bay, Long Island Sound and Prince Edward Island. Occurrence followed a seasonal distribution. Low densities were observed in June, increased through August, and became rare by September. Given the ubiquitous nature of marine Vibrios, it was not surprising to find V. vulnificus everywhere, which was investigated. Observations confirmed the importance of strict time/temperature and product handling practices by shellfish harvesters, dealers and processors. Mishandling led to a potentially dangerous and uncharacteristically high V. vulnificus observation.


The Anti-Microbial Efficacy Of Plant Essential Oil Combinations And Interactions With Food Ingredients, Jorge Gutierrez, Catherine Barry-Ryan, Paula Bourke May 2008

The Anti-Microbial Efficacy Of Plant Essential Oil Combinations And Interactions With Food Ingredients, Jorge Gutierrez, Catherine Barry-Ryan, Paula Bourke

Articles

The objective of this study was to evaluate the efficacy of plant essential oils (EOs) in combination and to investigate the effect of food ingredients on their efficacy. The EOs assessed in combination included basil, lemon balm, marjoram, oregano, rosemary, sage and thyme. Combinations of EOs were initially screened against Bacillus cereus, Escherichia coli, Listeria monocytogenes and Pseudomonas aeruginosa using the spot-on-agar test. The influence of varying concentrations of EO combinations on efficacy was also monitored using E. coli. These preliminary studies showed promising results for oregano in combination with basil, thyme or marjoram. The checkerboard method was then used …


Unl Announces Opening Of Core For Applied Genomics And Ecology (Cage), Andy Benson Jan 2008

Unl Announces Opening Of Core For Applied Genomics And Ecology (Cage), Andy Benson

Core for Applied Genomics and Ecology (CAGE)

The UNL Core for Applied Genomics and Ecology (CAGE) announces that it is now offering a new suite of services to UNL investigators and outside entities. Formerly known as the UNL Printed Microarray Core Facility, CAGE now offers Next Gen sequencing on its newly installed Roche-454 GS FLX. In support of the UNL Gut Function Initiative, CAGE specializes in application of the GS-FLX platform for analysis of complex microbial communities by massively parallel sequencing of 16S rRNA amplicons. CAGE also has expertise in shotgun sequencing applications on the instrument, including whole genome sequencing and analysis of BACs and metagenome libraries. …


Next Gen Sequencing: An Alternative To Culture-Based Microbiological Testing Jan 2008

Next Gen Sequencing: An Alternative To Culture-Based Microbiological Testing

Core for Applied Genomics and Ecology (CAGE)

Next Gen DNA Sequencing Enables You...
• to analyze microbial communities and ecosystems in food products
• perform food safety testing
• address spoilage and other non-safety issues
• optimize food processing
• create databases of “good” microbial profiles associated with target product characteristics


Aislamiento E Identificación De Bacterias Ácido Lácticas A Partir De Leche Cruda Y Queso Paipa Elaborado En Los Municipios De Pacho Cundinamarca Y Belén Boyacá, Juan Carlos Suarez Murcia Jan 2008

Aislamiento E Identificación De Bacterias Ácido Lácticas A Partir De Leche Cruda Y Queso Paipa Elaborado En Los Municipios De Pacho Cundinamarca Y Belén Boyacá, Juan Carlos Suarez Murcia

Zootecnia

La fermentación de los alimentos por bacterias ácido lácticas es una de las formas más antiguas de conservación usadas por el hombre. Uno de los alimentos resultante de dichas fermentaciones es el queso Paipa, el cual es uno de los productos mas representativos de nuestro país en cuanto a quesos semimadurados, desafortunadamente, este tipo de queso se produce en forma artesanal, haciéndolo riesgoso para la salud pública, debido a que la leche utilizada en este proceso no es sometida a tratamiento térmico. En el presente trabajo, se realizaron análisis físico-químicos a la leche y al queso Paipa identificándolo como un …


Investigating The Effectiveness Of Malic Acid, Nisin, And Grape Seed Extract Incorporated Into Whey-Protein Coatings To Inhibit The Growth Of Listeria Monocytogenes On Ready-To-Eat Poultry, Amanda Bettasso, Navam Hettiarachychy, Vidya Chitturi, Michael Johnson Jan 2008

Investigating The Effectiveness Of Malic Acid, Nisin, And Grape Seed Extract Incorporated Into Whey-Protein Coatings To Inhibit The Growth Of Listeria Monocytogenes On Ready-To-Eat Poultry, Amanda Bettasso, Navam Hettiarachychy, Vidya Chitturi, Michael Johnson

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

The ability to control growth of Listeria monocytogenes on ready-to-eat poultry products with the antimicrobials nisin, malic acid, and grape seed extract incorporated into whey-protein coatings was evaluated. The antimicrobials were incorporated into the coating solution alone and in combinations. One gram pieces of turkey frankfurters were coated with the coating solutions and then inoculated with L. monocytogenes and stored at 4°C for 28 days. The inhibitory effect of the coatings on turkey frankfurter pieces was evaluated on d 0, 7, 14, 21, and 28. Coatings containing 2% malic acid, 3% malic acid, and the combination of nisin (6,000 IU/g) …


Optimisation Of Steamer Jet-Injection To Extend The Shelf Life Of Fresh-Cut Lettuce, Daniel Rico, Ana Belen Martin-Diana, Catherine Barry-Ryan, Gary Henehan, J. Barat, Jesus Maria Frias Jan 2008

Optimisation Of Steamer Jet-Injection To Extend The Shelf Life Of Fresh-Cut Lettuce, Daniel Rico, Ana Belen Martin-Diana, Catherine Barry-Ryan, Gary Henehan, J. Barat, Jesus Maria Frias

Articles

Optimisation of short time blanching (steaming) was investigated using response surface methodology by analysing quality and microbial and nutritional markers over the shelflife of packaged fresh-cut lettuce. Steamer treatment time (5–10 s) and storage (1–10 days) were used as independent factors in order to optimise the process. Longer treatments (>10 s) were not feasible because of extensive damage caused to lettuce tissue. Significantly (p < 0.05) higher values of luminosity (L*), greenness (−a*) and sensory panel scores (fresh appearance, general acceptability and absence of browning) were obtained with samples treated for longer times with the steamer. Activity of browning-related enzymes present in photosynthetic tissue decreased in all the cases, however, longer treatment time (7.5–10 s) was needed with vascular tissues to reduce to similar activity values. These differences could be explained by the variation in tissue thickness. The longest steam treatment (10 s) reduced and maintained significantly (p < 0.05) lower mesophilic load than shorter treatments (5–8.5 s). However, significant (p < 0.05) decreases in vitamin C and carotenoids were observed in samples treated with longer treatments. Steamer treatment of 10 s could be considered the optimum time for maintaining the shelflife (mainly texture and browning) of fresh-cut lettuce for 7–10 days in optimum conditions.