Entomology Commons^™

Exploring A Novel Substrate-Borne Vibratory Signal In The Wolf Spider Schizocosa Floridana, Malcolm F. Rosenthal, Eileen Hebets, Rowan H. Mcginley, Cody Raiza, James Starrett, Lin Yan, Damian O. Elias

Eileen Hebets Publications

Animals communicate using a diversity of signals produced by a wide array of physical structures. Determining how a signal is produced provides key insights into signal evolution. Here, we examine a complex vibratory mating display produced by male Schizocosa floridana wolf spiders. This display contains three discrete substrate-borne acoustic components (known as “thumps”, “taps”, and “chirps”), each of which is anecdotally associated with the movement of a different body part (the pedipalps, legs, and abdomen respectively). In order to determine the method of production, we employ a combination of high-speed video/audio recordings and SEM imaging of possible sound-producing structures. Previous …

Testing The Hypothesized Antipredator Defence Function Of Stridulation In The Spiny Orb-Weaving Spider, Micrathena Gracilis, Tyler B. Corey, Eileen A. Hebets

Eileen Hebets Publications

The observable diversity of antipredator defenses across organisms demonstrates predation’s impact on trait evolution. The functions of many traits that are presumed to have an antipredator function have never been directly tested. The spiny orb-weaving spider, Micrathena gracilis, for example, stridulates when grasped. While stridulation was first hypothesized to be an antipredator defense nearly 50 years ago, no data exist to support this hypothesis. To explore the form and function of M. gracilis stridulation, we first quantified the behavioral and acoustical properties of sound production. Next, using laboratory assays, we directly tested the effect of stridulation on survival with …

First Report Of A Gall Midge As A Parasitoid Of Weaver Ants, Bethelihem Mekonnen, Julien Haran, Robert S. Copeland, Christian W.W. Pirk, Abdullahi A. Yusuf, Emilie Deletre

All Peer-Reviewed Publications

Gall midges (Diptera: Cecidomyiidae) comprise a diverse family of small nematoceran flies. While most species are phytophagous, the family also includes predatory species, and endoparasitoids. Endoparasitic species have been reared from aphids and psyllids. We discovered parasitoid-infected weaver ant, Oecophylla longinoda (Latreille) (Hymenoptera: Formicidae) in nests on mango trees in coastal Kenya. Examination of 5 colonies revealed the presence of parasitoids in all of them, with parasitism rates ranging from 2 to 21%. Morphological examination of larval parasitoids indicated that they were gall midges, and molecular data were consistent with the identification. Attempts to rear adults were unsuccessful. This study …

First Report Of Brachyplatys Subaeneus (Westwood) (Hemiptera: Heteroptera: Plataspidae) In The United States, Joseph E. Eger Jr., Adam J. Pitcher, Susan E. Halbert, Cory Penca, Amanda C. Hodges

Insecta Mundi

Brachyplatys subaeneus (Westwood) (Hemiptera: Plataspidae), is reported from Miami Beach, FL, the first report from the United States. It was noticed first on a photo-sharing site (iNaturalist) providing an example of the importance of these sites for helping to track introduced species. Information on distribution, host plants and potential spread are provided along with photos and taxonomic characters to assist with identification of this recently introduced species.

ZooBank registration. urn:lsid:zoobank.org:pub:332C0E76-9AE9-4069-9240-F55CADC64165

Checklist Of The Caddisfly Family Rhyacophilidae (Insecta: Trichoptera) In India, Tabaraq Ali, Sajad H. Parey, Manpreet S. Pandher, Malkiat S. Saini

Insecta Mundi

The present checklist covers the fauna of the Trichopteran family Rhyacophilidae Stephens, 1836. Based on extensive surveys done during 2003–2019 and previous species records, we find that the family Rhyacophilidae is represented by two genera and 184 species in India. The genus Rhyacophila Pictet contains 165 species in India while the genus Himalopsyche Banks contains 19 described species. The Indian Himalayan region is inhabited by more species than the rest of India.

A New Species Of Parorectis Spaeth From The North-Central United States, With Notes On Prothoracic And Head Morphology Of The Genus (Coleoptera: Chrysomelidae: Cassidinae: Cassidini), Edward G. Riley

Insecta Mundi

A new species of tortoise beetle from the north-central United States, Parorectis arenaria new species (Coleoptera: Chrysomelidae: Cassidinae: Cassidini), is described and illustrated. Comparative remarks and a key are provided to distinguish the new species from the three other species comprising the genus. Adults and larvae of the new species feed on Physalis L. (Solanaceae). The circum-foraminal ridge and antennal groove of the prothorax are described in detail. The pars stridens (file) of the stridulatory apparatus on the male cranium of the new species is illustrated. The pars stridens is present only in males of Parorectis Spaeth species. A patch …

First Record For Cypherotylus Dromedarius (Lacordaire) (Coleoptera: Erotylidae) From Panama, Brian J. Armitage, Alonso Santos Murgas

Insecta Mundi

Cypherotylus dromedarius (Lacordaire, 1842) (Coleoptera: Erotylidae) was described from French Guiana. Information about its current distribution is scattered throughout the literature and the internet. Specimens of this species were recently observed and photographed in the Boquete area, Chiriqui Province, Panama, and constitute a new record for the country of this species. Subsequently, we located two specimens deposited in the National Reference Collection of the G. B. Fairchild Museum of Invertebrates, University of Panama, from the same locality in Chiriqui Province. We summarize the distribution of this species from scattered sources and provide five new records.

Cypherotylus dromedarius (Lacordaire, 1842) (Coleoptera: …

Additional Type Designations For Fireflies (Coleoptera: Lampyridae) Of The Biologia Centrali Americana Housed In The Natural History Museum, London, Gavin J. Martin, Natalie A. Saxton

Insecta Mundi

Firefly (Coleoptera: Lampyridae) taxonomy has undergone numerous changes over the past 100 years. In order to help provide stability to the group, types for several of the Lampyridae of the Biologia Centrali Americana were determined or designated in early 2019. Here we provide treatments for the remaining Lampyridae and determine the holotype specimens for four species and designate lectotype specimens for 33 species.

ZooBank registration. urn:lsid:zoobank.org:pub:1EBF8DAB-3EC5-4671-9E15-C3D2213E3164

The Genus Ochodaeus In Italy: Taxonomy And Distribution (Coleoptera: Scarabaeoidea: Ochodaeidae), Stefano Ziani

Insecta Mundi

The author provides a taxonomic, nomenclatural and distributional review of the genus Ochodaeus Dejean, 1821 (Coleoptera: Scarabaeoidea: Ochodaeidae) in Italy. All Italian populations have been confirmed to belong to a single species, O. chrysomeloides (Schrank, 1781). After the study of a syntype, O. cychramoides Reitter, 1892, formerly considered an Italian endemic, is confirmed to be a junior synonym of O. chrysomeloides. Type material of O. chrysomeloides is believed to be destroyed, therefore a neotype is here designated and deposited at the Natural History Museum of Vienna, Austria. A lectotype is here designated for O. cychramoides and deposited in the Hungarian …

A New Central American Genus Of Pleasing Fungus Beetles (Coleoptera: Erotylidae) From The Ischyrus-Megischyrus Complex, Paul E. Skelley

Insecta Mundi

Antoinettia, new genus (Coleoptera: Erotylidae: Erotylinae: Tritomini), is erected for three species: A. audbala (Skelley), new combination, A. huhnei Skelley, new species, and A. kovariki (Skelley), new combination. A genus complex involving Ischyrus Lacordaire, 1842, and Megischyrus Crotch, 1873, is defined and a preliminary key to neotropical genera of Tritomini with coarsely facetted eyes is presented.

ZooBank registration. urn:lsid:zoobank.org:pub:7CF5D30C-C46E-4135-A188-B53B0A77B34E

Dos Nuevas Especies De Gorgojos Que Barrenan Los Frutos De Palma De Chontaduro De Los Géneros Parisoschoenus Y Cylindrocerus (Coleoptera: Curculionidae: Baridinae) De La Costa Pacífica De Colombia, Luis Miguel Constantino, Luis Carlos Pardo-Locarno

Insecta Mundi

Actualmente existen dos especies de gorgojos (Coleoptera: Curculionidae) que barrenan los frutos de la palma de chontaduro Bactris gasipaes Kunth en Colombia y que ocasionan pérdidas de hasta el 100% de la producción. Palmelampius heinrichi O’Brien es la principal plaga del cultivo en toda la costa pacífica y en vastas regiones de la amazonia y el piedemonte llanero. En este trabajo se describe una nueva especie del género Parisoschoenus Faust, P. bactrisae Constantino y Pardo-Locarno que ocurre simpátricamente con P. heinrichi en la costa pacífica de Colombia causando un daño similar y que se diferencia de la especie anterior por …

Using Dna Barcoding For Identification Of Some Psyllids (Hemiptera: Psylloidea) Intercepted At South Korea Ports-Of-Entry, Soo-Jung Suh, Deuk-Soo Choi

Insecta Mundi

Quick and accurate identification of intercepted psyllids (Hemiptera: Psylloidea) is an essential requirement for effective pest management and phytosanitary procedures. However, due to lack of morphological characters in the immature stages that can be used to distinguish species, other molecular methods, such as DNA barcoding are proving to be useful. The current study was to designed to generate comprehensive information on the identification of all developmental stages of eight species of psyllids intercepted on consignments of infested fresh cut flowers at the ports of entry in South Korea using DNA barcoding. It is considered that DNA barcoding is a reliable …

Comparison Of Native And Non-Native Predator Consumption Rates And Prey Avoidance Behavior In North America And Europe, Ayse Gül Ünlü, John J. Obrycki, Roman Bucher

Entomology Faculty Publications

Novel predator–prey interactions can contribute to the invasion success of non-native predators. For example, native prey can fail to recognize and avoid non-native predators due to a lack of co-evolutionary history and cue dissimilarity with native predators. This might result in a competitive advantage for non-native predators. Numerous lady beetle species were globally redistributed as biological control agents against aphids, resulting in novel predator–prey interactions. Here, we investigated the strength of avoidance behavior of the pea aphid (Acyrthosiphon pisum) toward chemical cues of native lady beetles and non-native Asian Harmonia axyridis and European Coccinella septempunctata and Hippodamia variegata …

Selection Of Reference Genes For Qrt-Pcr Analysis In Medicinal Plant Glycyrrhiza Under Abiotic Stresses And Hormonal Treatments, Yuping Li, Xiaoju Liang, Xuguo Zhou, Zhigeng Wu, Ling Yuan, Ying Wang, Yongqing Li

Entomology Faculty Publications

Best known as licorice, Glycyrrhiza Linn., a genus of herbaceous perennial legume, has been used as a traditional herbal medicine in Asia and a flavoring agent for tobacco and food industry in Europe and America. Abiotic stresses and hormonal treatments can significantly impact the development and metabolism of secondary metabolites in Glycyrrhiza. To better understand the biosynthesis of the trace-amount bioactive compounds, we first screened for the suitable reference genes for quantitative real-time reverse transcription PCR (qRT-PCR) analysis in Glycyrrhiza. The expression profiles of 14 candidate reference genes, including Actin1 (ACT), Clathrin complex AP1 (CAC), Cyclophilin (CYP), Heat-shock …

Identification Of Planktothrix (Cyanobacteria) Blooms And Effects On The Aquatic Macroinvertebrate Community In The Non-Tidal Potomac River, Usa, Joshua Henesy, Jennifer L. Wolny, John E. Mullican, Detbra S. Rosales, Joseph S. Pitula, Joseph W. Love

Virginia Journal of Science

Using transverse cross-sectional transects, a survey of 31 km of the non-tidal Potomac River was conducted from White’s Ferry, Virginia to Brunswick, Maryland, USA, between June and September in 2013 through 2015 to assess a recurring benthic cyanobacteria bloom. Abundant benthic cyanobacteria blooms were detected during the 2014 and 2015 sampling seasons and the primary taxon was identified morphologically and molecularly as Planktothrix cf. isothrix. When present, P. cf. isothrix blooms were concentrated from river center to the Maryland shoreline. This pattern was correlated with significantly greater benthic chlorophyll-a and phycocyanin concentrations. In an apparent response to …

A New Species Of Diolcus Mayr (Hemiptera: Heteroptera: Scutelleridae: Pachycorinae) With A Reexamination Of The Subfamily And Generic Placement Of Nesogenes Boscii (Fabricius) (Hemiptera: Heteroptera: Scutelleridae: Elvisurinae Or Pachycorinae), Joseph E. Eger Jr.

Insecta Mundi

Diolcus thomasi Eger new species (Hemiptera: Heteroptera: Scutelleridae: Pachycorinae), is de­scribed from Cayman Brac and Little Cayman Island and compared to D. chrysorrhoeus (Fabricius), its closest congener. Nesogenes boscii (Fabricius) is removed from the Elvisurinae and placed in the Pachycorinae. The relationship of N. boscii to D. thomasi and D. chrysorrhoeus is discussed.

Diolcus Mayr (Hemiptera: Heteroptera: Scutelleridae: Pachycorinae) currently contains six valid species: D. cassi­doides (Walker) from Hispaniola, D. chrysorrhoeus (Fabricius) from the southeastern US and Texas, D. disjunctus Barber from Puerto Rico, D. irroratus (Fabricius) from Florida and many of …

Suitability Of Native Milkweed (Asclepias) Species Versus Cultivars For Supporting Monarch Butterflies And Bees In Urban Gardens [Research Data], Daniel A. Potter

Entomology Research Data

Public interest in ecological landscaping and gardening is fueling a robust market for native plants. Most plants available to consumers through the horticulture trade are cultivated forms that have been selected for modified flowers or foliage, compactness, or other ornamental characteristics. Depending on their traits, some native plant cultivars seem to support pollinators, specialist insect folivores, and insect-based vertebrate food webs as effectively as native plant species, whereas others do not. There is particular need for information on whether native cultivars can be as effective as true or “wild-type” native species for supporting specialist native insects of conservation concern. Herein …

Evaluation Of Systemic Imidacloprid And Herbicide Treatments On Flatheaded Borer (Coleoptera: Buprestidae) Management In Field Nursery Production, Karla M. Addesso, Jason B. Oliver, Nadeer N. Youssef, Donna C. Fare

Agricultural and Environmental Sciences Faculty Research

The flatheaded appletree borer, Chrysobothris femorata (Olivier) (Coleoptera: Buprestidae), and related species are deciduous tree pests. Female beetles prefer to oviposit at tree bases, and larvae tunnel beneath the bark, which weakens or kills young or newly transplanted trees. In the first objective of this study, Discus N/G (2.94% imidacloprid + 0.7% cyfluthrin) applied at six lower-than-labeled rates (0.0, 0.98, 1.97, 3.94, 5.91, and 7.87 ml/cm of average trunk dia.) was evaluated for protection of field-grown maples. A second objective evaluated imidacloprid with and without herbicides to assess the impact of weed competition at the tree base on insecticide effectiveness. …

The Impact Of Plant Essential Oils And Fine Mesh Row Covers On Flea Beetle (Chrysomelidae) Management In Brassicaceous Greens Production, Robert Brockman, Ryan Kuesel, Kendall Archer, Kyla O’Hearn, Neil Wilson, Delia W. Scott, Mark A. Williams, Ricardo Bessin, David J. Gonthier

Entomology Faculty Publications

Brassicaceous leafy greens are an important crop for small growers but are difficult to produce due to damage by flea beetles. Flea beetles are problematic for growers as they chew many small holes through leaves rendering produce unmarketable. We tested the efficacy of several essential oils, the woven-mesh row cover ProtekNet, and the spunbonded row cover Agribon, compared to organic and conventional insecticides and no spray controls in the spring and fall of 2019. We found that the two row cover treatments (Agribon and ProtekNet) provided the best control of flea beetles and associated damage. Thyme oil was highly phytotoxic …

Description Of A New Species Of Chrysina Kirby (Coleoptera: Scarabaeidae: Rutelinae) From The Sierra Azul, Oaxaca, Mexico, A New Synonymy, And Notes On Chrysina Species Found In The Sierra Azul, José Monzón Sierra, Julian Federico Blackaller-Bages, David C. Hawks

Insecta Mundi

A new species of Chrysina Kirby, C. clavellina Monzón, Blackaller, and Hawks (Coleoptera: Scarabaeidae: Rutelinae: Rutelini) is described from Santiago Clavellinas in the Sierra Azul in Oaxaca, Mexico. Chrysina cosijoezai (Ramírez-Ponce and Curoe) is placed as a junior synonym of C. lacordairei (Boucard), and notes on the Chrysina fauna of this mountain range are included.

Se describe una nueva especie de Chrysina Kirby, C. clavellina Monzón, Blackaller, y Hawks (Coleoptera: Scarabaeidae: Rutelinae) de Santiago Clavellinas en la Sierra Azul en Oaxaca, México. Chrysina cosijoezai (Ramírez-Ponce y Curoe) se sitúa como un sinónimo de C. lacordairei (Boucard) y se incluyen notas …

A New Cryptic Species Of Metallic Chrysina Kirby (Coleoptera: Scarabaeidae: Rutelinae) From Western Honduras, José Monzón Sierra, David C. Hawks

Insecta Mundi

A new species of Chrysina Kirby (Coleoptera: Scarabaeidae: Rutelinae), Chrysina porioni Monzón and Hawks, is described from the Sierra del Merendón west of San Pedro Sula, Honduras. This species is very similar to C. ericsmithi (Monzón and Cano), with which it has long been confused.

Resumen. Se describe una nueva especie de Chrysina Kirby (Coleoptera: Scarabaeidae: Rutelinae), Chrysina porioni Monzón y Hawks, de la Sierra del Merendón al oeste de San Pedro Sula, Honduras. Esta especies es muy parecida a C. ericsmithi (Monzón y Cano), con la cuál se ha confundido por mucho tiempo.

Nebline, September/October 2020

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Fall Invaders: What You Can Do

Extension Partners With LPS on Handwashing Videos

Plant A Row for the Hungry

Recipe of the Month

Pruning Everbearing Raspberries

Seasonal Needle Drop on Evergreens

Garden Guide: Things to Do This Month

Disasters Don't Wait. Make Your Plan Today!

Extension Offers Training for Emergency Preparedness and Coping

Upcoming Early Childhood Trainings

Nitrate in Drinking Water

Heart of 4-H Volunteer Award: Tracy Pracheil

Heart of 4-H Volunteer Award: Amy Vander Woude

4-H Announcements for enrolled 4-H'ers or 4-H volunteers

4-H Information Night

State 4-H Horse Expo Top Results

4-H at Super Fair a Blend of …

New Records Document Cystiphora Sonchi (Vallot) (Diptera: Cecidomyiidae) And Associated Parasitoids (Hymenoptera) In The Continental United States, Louis S. Hesler, Michael W. Gates, Eric A. Beckendorf

Insecta Mundi

A gall midge, Cystiphora sonchi (Vallot, 1827) (Diptera: Cecidomyiidae), is reported for the first time in the continental United States of America from the states of Minnesota, North Dakota, and South Dakota. The gall midge is an obligate parasite of Sonchus L., including perennial sowthistle, Sonchus arvensis L., a weed that was the impetus for earlier releases of C. sonchi as a biological control in Canada. Patches of S. arvensis were commonly infested with C. sonchi, and often leaves were densely galled. Dissections of galled leaves led to the novel finding of multiple C. sonchi larvae in some individual …

Reproductive Compatibility Among Populations And Host‐Associated Lineages Of The Common Bed Bug (Cimex Lectularius L.), Zachary C. Devries, Richard G. Santangelo, Warren Booth, Christopher G. Lawrence, Ondřej Balvín, Tomáš Bartonička, Coby Schal

Entomology Faculty Publications

As populations differentiate across geographic or host‐association barriers, interpopulation fertility is often a measure of the extent of incipient speciation. The bed bug, Cimex lectularius L., was recently found to form two host‐associated lineages within Europe: one found with humans (human‐associated, HA) and the other found with bats (bat‐associated, BA). No unequivocal evidence of contemporary gene flow between these lineages has been found; however, it is unclear whether this is due to an inability to produce viable “hybrid” offspring. To address this question and determine the extent of compatibility between host‐associated lineages, we set up mating crosses among populations of …

Interplay Of Phytohormones Facilitate Sorghum Tolerance To Aphids, Sajjan Grover, Earl Agpawa, Gautam Sarath, Scott E. Sattler, Joe Louis

Department of Entomology: Faculty Publications

Key message -- Interactions among phytohormones are essential for providing tolerance of sorghum plants to aphids.

Plant’s encounter with insect herbivores trigger defense signaling networks that fine-tune plant resistance to insect pests. Although it is well established that phytohormones contribute to antixenotic- and antibiotic-mediated resistance to insect pests, their role in conditioning plant tolerance, the most durable and promising category of host plant resistance, is largely unknown. Here, we screened a panel of sorghum (Sorghum bicolor) inbred lines to identify and characterize sorghum tolerance to sugarcane aphids (SCA; Melanaphis sacchari Zehntner), a relatively new and devastating pest of sorghum in …

Population Abundance Of Varroa Destructor And Its Effects On Apis Mellifera Scutellata Colonies In Kenya, Sammy Kiprotich Cheruiyot, Ruth Kahuthia-Gathu, Jenard Patrick Mbugi, Elliud Muli, H. Michael G. Lattorff

All Peer-Reviewed Publications

The devastating effects of Varroa destructor Anderson and Trueman on Western honeybee colonies (Apis mellifera L.) have been well documented. Not only do these mites cause physical damage to parasitized individuals when they feed on them, but they also transmit viruses and other pathogens, weaken colonies and ultimately may cause their death. Unlike the subspecies of European origin, the honeybees of African origin suffer less from mite infestations. Absconding is one of the factors contributing to low V. destructor population in honeybee colonies as it creates a brood-free period. For a long time, researchers hypothesized that absconding was the main …

Interactions Between Two Parasitoids Of Tephritidae: Diachasmimorpha Longicaudata (Ashmead) And Psyttalia Cosyrae (Wilkinson) (Hymenoptera: Braconidae), Under Laboratory Conditions, Shepard Ndlela, Samira Abuelgasim Mohamed, Abdelmutalab G.A. Azrag, Paul Nduati Ndegwa, George Otieno Ong’Amo, Sunday Ekesi

All Peer-Reviewed Publications

The braconid wasp, Diachasmimorpha longicaudata (Ashmead), was introduced in Kenya from Hawaii for classical biological control of the invasive tephritid, Bactrocera dorsalis Hendel. Following reports that D. longicaudata had formed new associations with Ceratitis cosyra, laboratory experiments were conducted to assess the interaction between the introduced and the native parasitoid of C. cosyra; Psyttalia cosyrae (Wilkinson) under three scenarios: B. dorsalis only, C. cosyra only and mixed populations of the two species. Parasitoids were introduced to the host as sole, sequential and simultaneous releases. Host searching and probing events were five times higher for D. longicaudata than P. cosyrae with …

Repellence And Fumigant Toxicity Of Essential Oils Of Ocimum Gratissimum And Ocimum Kilimandscharicum On Tuta Absoluta (Lepidoptera: Gelechiidae), Flaure Rosette Ehawa Essoung, Alain Tcho Tadjong, Sumesh Chander Chhabra, Samira Abuelgasim Mohamed, Ahmed Hassanali

All Peer-Reviewed Publications

Tuta absoluta Meyrick originates in South America and is now one of the most important insect pests of Solanaceae in different parts of the world, including Africa. Its control has relied primarily on chemical insecticides, which are associated with negative ecological effects. In the present study, essential oils of Ocimum gratissimum and O. kilimandscharicum were tested for repellence and fumigant toxicity on the adult stages under laboratory conditions. The oil of O. gratissimum was more repellent, but its toxicity was comparable with that of O. kilimandscharicum. The major constituents of O. gratissimum were methyl eugenol (39.5%) and eugenol (29.7%). Those …

Two New Species Of Corynellus Bates, 1885 (Coleoptera: Cerambycidae: Cerambycinae), Antonio Santos-Silva, James E. Wappes

Insecta Mundi

Two new species of Corynellus (Coleoptera: Cerambycidae: Cerambycinae) are described: C. rebeccae Santos-Silva and Wappes, from Bolivia; and C. elianae Santos-Silva and Wappes, from Panama. A key to species of Corynellus is provided.

The Matsucoccus Cockerell, 1909 Of Florida (Hemiptera: Coccomorpha: Matsucoccidae): Potential Pests Of Florida Pines, Muhammad Z. Ahmed, Charles H. Ray, Matthew R. Moore, Douglass R. Miller

Insecta Mundi

Matsucoccus krystalae Ahmed and Miller, new species, (Hemiptera: Coccomorpha: Matsucoccidae) is described based on morphological characters of adult females and third-instar males. We designate the lectotype of Matsucoccus alabamae Morrison. Matsucoccus alabamae Morrison and M. gallicolus Morrison are redescribed, also based on adult females and third-instar males. Detailed illustrations and descriptions are presented for each species and an identification key for the five species occurring in the eastern U.S. is provided. Analysis of 18S, 28S D2/D3, and 28S D10 loci were performed to support morphological determination. Barcodes using 5′ COI of M. alabamae and M. krystalae were generated, the …