Entomology Commons^™

Ectoparasite Presence In Select Northcentral Kansas Bat Species, Elizabeth Schumann

Master's Theses or Doctor of Nursing Practice

Working with other graduate students on a grant given to Fort Hays State University, from the Kansas Department of Wildlife, Parks and Tourism, I looked at presence and species of ectoparasites on bat species. The main goal of our grant was to quantify and qualify the status of the northern myotis (Myotis septentrionalis) in the state of Kansas, and to record data on any bycatch. I worked on our grant in the summer field seasons of 2016 and 2017, May to October, as described by the Indiana bat protocol.

Bats were captured by using mist nets set over …

Opda Regulates Maize Defense Against Aphids, Suresh Varsani, Sajjan Grover, Shaoqun Zhou, Kyle G. Koch, Pei-Cheng Huang, Michael V. Kolomiets, W. Paul Williams, Tiffany Heng-Moss, Gautam Sarath, Dawn S. Luthe, Georg Jander, Joe Louis

Department of Entomology: Faculty Publications

Copyright © 2019 American Society of Plant Biologists.

DOI:10.1104/pp.18.01472

Rnai Targeting Of Rootworm Troponin I Transcripts Confers Root Protection In Maize, Elane Fishilevich, Andrew J. Bowling, Meghan L. F. Frey, Po-Hao Wang, Wendy Lo, Murugesan Rangasamy, Sarah E. Worden, Heather E. Pence, Premchand Gandra, Shannon L. Whitlock, Greg Schulenberg, Eileen Knorr, Linda Tenbusch, Jamie R. Lutz, Stephen Novak, Ronda L. Hamm, Karl D. Schnelle, Andreas Vilcinskas, Kenneth E. Narva

Department of Entomology: Faculty Publications

Western corn rootworm, Diabrotica virgifera virgifera, is the major agronomically important pest of maize in the US Corn Belt. To augment the repertoire of the available dsRNA-based traits that control rootworm, we explored a potentially haplolethal gene target, wings up A (wupA), which encodes Troponin I. Troponin I, a component of the Troponin-Tropomyosin complex, is an inhibitory protein involved in muscle contraction. In situ hybridization showed that feeding on wupA-targeted dsRNAs caused systemic transcript knockdown in D. v. virgifera larvae. The knockdown of wupA transcript, and by extension Troponin I protein, led to deterioration of the …

P7 And P8 Proteins Of High Plains Wheat Mosaic Virus, A Negative-Strand Rna Virus, Employ Distinct Mechanisms Of Rna Silencing Suppression, Adarsh K. Gupta, Gary L. Hein, Satyanarayana Tatineni

Department of Entomology: Faculty Publications

High Plains wheat mosaic virus (genus Emaravirus), an octapartite negative-sense RNA virus, encodes two RNA silencing suppressors, P7 and P8. In this study, we found that P7 and P8 efficiently delayed the onset of dsRNA-induced transitive pathway of RNA silencing. Electrophoretic mobility shift assays (EMSA) revealed that only P7 protected long dsRNAs from dicing in vitro and bound weakly to 21- and 24-nt PTGS-like ds-siRNAs. In contrast, P8 bound strongly and relatively weakly to 21- and 24-nt ds-siRNAs, respectively, suggesting size-specific binding. In EMSA, neither protein bound to 180-nt and 21-nt ssRNAs at detectable levels. Sequence analysis revealed that …

Age- And Sex-Related Abc Transporter Expression In Pyrethroid-Susceptible And – Resistant Aedes Aegypti, Leslie C. Rault, Ellis J. Johnson, Scott T. O'Neal, Rui Chen, Sarah E. Mccomic, Daniel R. Swale, Troy D. Anderson

Department of Entomology: Faculty Publications

Resistance mechanisms to synthetic insecticides often include point mutations and increased expression of genes encoding detoxification enzymes. Since pyrethroids are the main adulticides used against Aedes aegypti, which vectors pathogens such as Zika virus, understanding resistance to this insecticide class is of significant relevance. We focused on adenosine triphosphate (ATP)-binding cassette (ABC) transporters in the pyrethroid-resistant Puerto Rico (PR) strain of Ae. aegypti. We investigated the expression patterns of six ABC transporters previously characterized as differentially expressed in insecticide-challenged mosquitoes, or increased mRNA expression in pyrethroid-resistant Ae. aegypti, by comparing PR to the Rockefeller (Rock) susceptible strain. …

Hypericum Kalmianum (St. Johnswort) Confirmed As A New Host Of The Crapemyrtle Bark Scale In Virginia, U.S.A., Allen Szalanski

Entomology and Plant Pathology Faculty Publications and Presentations

Crapemyrtle bark scale, Acanthococcus lagerstroemiae (Hemiptera: Eriococcidae), is an invasive pest of crapemyrtle (Lagerstroemia spp.) that has expanded its range across the southeastern United States since its initial detection in 2004. Although previously considered host-specific to crapemyrtle in the United States, limited reports have suggested a broader host range. In 2018, infestations of scale insects were observed on Hypericum kalmianum (‘Blue Velvet’) at the Hampton Roads Agricultural Research and Extension Center in Virginia Beach, VA. Morphological identification and mitochondrial COI DNA sequencing confirmed the specimens as A. lagerstroemiae, with 99.5% sequence similarity to published GenBank records. Approximately 20% …

An Ecological Survey Of Forest Succession In A Northeast Ohio Ecosystem Following An Emerald Ash Borer Infestation, Joseph Haas

Williams Honors College, Honors Research Projects

This paper will be an ecological survey of a selected 28 acre piece of land in northeast Ohio impacted by the Emerald Ash Borer. This invasive species arrived in North America in 2002 and has since spread throughout the country wreaking havoc on the ash tree population. The purpose of this study is to determine what tree species are replacing the native ash trees in the succession of the forest. In order to achieve this goal, a section of the selected land will be blocked off and subdivided into manageable sections to facilitate data collection, namely the location of the …

Susceptibility Of Corn To Stink Bug (Dichelops Melacanthus) And Its Management Through Seed Treatment, Paulo Roberta Da Silva, Ademar Novais Istchuk, Thomas E. Hunt, Cristina Schetino Bastos, Jorge Braz Torres, Karolayne Lopes Campos, Josemar Foresti

Department of Entomology: Faculty Publications

We determined the susceptibility of vegetative corn stages to Dichelops melacanthus damage, and how seed treatment can reduce damage and yield loss. Two field trials were carried out. In the first, corn plants were artificially infested with D. melacanthus male/female pairs at rate of 0.5 pair per plant at different vegetative stages and infestation periods lasting 7-28 days (V1-V3, V1-V5, V1-V7, V1-V9, V3-V5, V3-V7, V3-V9, V5-V7, V5-V9, and V7-V9), plus a control without infestation. In the second, corn plants were artificially infested at a rate of one male/female pair per plant at different vegetative stages and infestation periods (V1-V3, V1-V5, …

A New Pest Species Of Resseliella (Diptera: Cecidomyiidae) On Soybean (Fabaceae) In North America, With A Description Of The Genus, Raymond Gagne, Junichi Yukawa, Ayman K. Elsayed, Anthony J. Mcmechan

Department of Entomology: Faculty Publications

A new species, Resseliella maxima Gagne, is reported that feeds on the lower stems of soybean, Glycine max (L.) Merr. (Fabaceae) in Nebraska, Iowa, South Dakota and Minnesota. It is described and illustrated and compared in detail with other Resseliella spp. DNA data support its status as a new species. A general description is given for Resseliella.

Wheat Streak Mosaic Virus Alters The Transcriptome Of Its Vector, Wheat Curl Mite (Aceria Tosichella Keifer), To Enhance Mite Development And Population Expansion, Adarsh K. Gupta, Erin D. Scully, Nathan A. Palmer, Scott M. Geib, Gautam Sarath, Gary L. Hein, Satyanarayana Tatineni

Department of Entomology: Faculty Publications

Wheat streak mosaic virus (WSMV; genus Tritimovirus; family Potyviridae) is an economically important wheat virus that is transmitted by the wheat curl mite (WCM; Aceria tosichella Keifer) in a persistent manner. Virus–vector coevolution may potentially influence vector gene expression to prolong viral association and thus increase virus transmission efficiency and spread. To understand the transcriptomic responses of WCM to WSMV, RNA sequencing was performed to assemble and analyse transcriptomes of WSMV viruliferous and aviruliferous mites. Among 7291 de novo-assembled unigenes, 1020 were differentially expressed between viruliferous and aviruliferous WCMs using edgeR at a false discovery rate ≤0.05. …

Biotic Potential And Reproductive Parameters Of Spodoptera Frugiperda (J. E. Smith, 1797) (Lepidoptera: Noctuidae), Débora Goulart Montezano, Alexandre Specht, Daniel R. Sosa-Gomez, Vânia F. Roque-Specht, Juaci V. Malaquias, Silvana L. Vieira De Paula Moraes, Julie A. Peterson, Thomas E. Hunt

Department of Entomology: Faculty Publications

The fall armyworm, Spodoptera frugiperda (J. E. Smith, 1797) (Lepidoptera: Noctuidae), a native to the Americas and recently reported in Africa, Germany, the Netherlands and India, is a significant pest of many crop species. Although a widespread and important pest, information on its biology and development are incomplete and require detailed study. In this study, the biotic potential and reproductive parameters of S. frugiperda were evaluated under controlled conditions (25 ± 1 °C, 70 ± 10% RH and 14 hour photophase). The longevity, pre-, post- and oviposition periods, fecundity, and fertility of 30 pairs were evaluated. The longevity of …

Composition And Host-Use Patterns Of A Scarab Beetle (Coleoptera: Scarabaeidae) Community Inhabiting The Canopy Of A Lowland Tropical Rainforest In Southern Venezuela, Susan Kirmse, Brett C. Ratcliffe

Department of Entomology: Faculty Publications

The adult scarab beetle fauna of the canopy in a lowland tropical rainforest in southern Venezuela was observed and collected by means of a 42 m-tall tower crane for a complete year. This first census of an entire Amazonian canopy scarab community was embedded within the interdisciplinary research project "Towards an understanding of the structure and function of a Neotropical rainforest ecosystem with special reference to its canopy" organized by the Austrian Academy of Science. The Scarabaeidae represented one of the most species-rich beetle families in the canopy of the crane plot and were therefore selected for a detailed analysis …

Soil Arthropod Abundance And Diversity Following Land Application Of Swine Slurry, Nichole R. Schuster, Julie A. Peterson, John E. Gilley, Linda R. Schott, Amy M. Schmidt

Department of Entomology: Faculty Publications

Soil arthropods play an important role in nutrient cycling and maintenance of soil structure, and their abundance and diversity provide an indication of the biological quality of soil. Land application of livestock manure provides crop nutrients and may also impact the soil arthropod community. This study was conducted to quantify soil arthropod abundance and diversity for a pe-riod of one year following swine manure application via broadcast or injec-tion. Arthropods were extracted from plot soil samples using Berlese funnels, identified and counted, and the QBS index (Qualità Biologica del Suolo) was calculated for each soil sample. Collembola (Hypogastruridae and Isotomi-dae) …

Effect Of Neem-Based Botanicals And Abamectin 1.8% Ec Against Phyllocnistis Citrella1 In Citrus Reticulata (Rutaceae) Nursery Plantations, Muhammad Arshad, Muhammad Irfan Ullah, Muhammad Afzal, Jaime Molina-Ochoa, Omar Francisco Prado-Rebolledo, John E. Foster

Department of Entomology: Faculty Publications

Citrus leafminer, Phyllocnistis citrella Stainton, is an economically important insect pest of citrus in Pakistan, Mexico, the United States and many other citrus-producing countries. Extract of Azadirachta indica A. Juss at 5 and 7% and its oil at 1 and 1.5% concentrations were tested in comparison to synthetic insecticide abamectin 1.8% EC against P. citrella in nursery plantations of Citrus reticulata L. Control of all larval instars of citrus leafminer was good with application of abamectin and A. indica oil during fall 2015 and summer 2016. Abamectin suppressed 75-90% of P. citrella larvae both seasons. A. indica oil at greater …

Temperature Dependent Development Of Sugarcane Aphids Melanaphis Sacchari, (Hemiptera: Aphididae) On Three Different Host Plants With Estimates Of The Lower And Upper Threshold For Fecundity, Michael A. De Souza, J. Scott Armstrong, W. Wyatt Hoback, Phillip G. Mulder, Sulochana Paudyal, John E. Foster, Mark E. Payton, Josephine Akosa

Department of Entomology: Faculty Publications

The sugarcane aphid, Melanaphis sacchari (Zehntner) is a serious economic threat to grain sorghum across the U.S. sorghum belt. It can develop on multiple grass hosts but only appears to survive winter temperatures in Texas near the Mexican border, Florida and Louisiana. Because survival and reproduction of aphids is dependent on access to appropriate nutrition and temperatures at which metabolic processes are maintained, we evaluated the growth, reproduction and survival of sugarcane aphids at constant temperatures (5°C,10°C,15°C,20°C,25°C,30°C,35°C) on three known hosts, sorghum, Johnsongrass, and Columbus grass. Longevity, fecundity, number of female nymphs/d, reproductive period in d, and intrinsic rate of …

Keys To Adults Of All Genera And Larvae Of 19 Species Of Gymnetini (Coleoptera: Scarabaeidae: Cetoninae) In The New World, With A Species Checklist And Descriptions Of Two New Genera And Species From Mexico And Mertinique, Brett C. Ratcliffe

Department of Entomology: Faculty Publications

Keys to adults of all 27 genera and larvae of 19 species in 10 genera of Gymnetini that occur in the New World are presented. Supplementing the key to adults is a checklist of all species, their synonyms, and all literature citations associated with the nomenclatural epithets. Two new genera, Gymnephoria Ratcliffe and Madiana Ratcliffe and Rome, with one new species each, are described from Mexico and Martinique, respectively.

Optimizing Egg Recovery From Wild Northern Corn Rootworm Beetles (Coleoptera: Chrysomelidae), Adriano E. Pereira, Dalton C. Ludwick, Julie Barry, Lance J. Meinke, Daniel J. Moellenbeck, Mark R. Ellersieck, Jordan D. Reinders, Ryan W. Geisert, Keiran Hyte, Amanda Ernwall, Kyle J. Paddock, Bruce E. Hibbard

Department of Entomology: Faculty Publications

The northern corn rootworm, Diabrotica barberi Smith & Lawrence (Coleoptera: Chrysomelidae), is one of the most important insect pests in the U.S. Corn Belt. Efforts to obtain eggs from wild northern corn rootworm populations using techniques developed for other rootworm species have been unsuccessful due to lack of oviposition. In 2016, we evaluated four oviposition media in choice tests within each of three female densities in 30.5 × 30.5 × 30.5 cm BugDorm cages. The number of eggs laid per female was significantly affected by female density and the interaction of female density × oviposition media, but oviposition was relatively …

Chlorothalonil Exposure Alters Virus Susceptibility And Markers Of Immunity, Nutrition, And Development In Honey Bees, Scott T. O'Neal, Alison M. Reeves, Richard D. Fell, Carlyle C. Brewster, Troy D. Anderson

Department of Entomology: Faculty Publications

Chlorothalonil is a broad spectrum chloronitrile fungicide that has been identified as one of the most common pesticide contaminants found in managed honey bees (Hymenoptera: Apidae: Apis mellifera L.), their food stores, and the hive environment. While not acutely toxic to honey bees, several studies have identified potential sublethal effects, especially in larvae, but comprehensive information regarding the impact of chlorothalonil on adults is lacking. The goal of this study was to investigate the effects of exposure to a field relevant level of chlorothalonil on honey bee antiviral immunity and biochemical markers of general and social immunity, as well as …

Community Composition, Abundance, And Phenology Of Stink Bugs (Hemiptera: Pentatomidae) In Soybean In The North Central Region Of The United States, Daniela Pezzini, Christina D. Difonzo, Deborah L. Finke, Thomas Hunt, Janet J. Knodel, Christian H. Krupke, Brian P. Mccornack, Andrew P. Michel, Christopher R. Philips, Adam J. Varenhorst, Robert J. Wright, Robert Koch

Department of Entomology: Faculty Publications

Stink bugs (Hemiptera: Pentatomidae) are an increasing threat to soybean (Fabales: Fabaceae) production in the North Central Region of the United States, which accounts for 80% of the country’s total soybean production. Characterization of the stink bug community is essential for development of management programs for these pests. However, the composition of the stink bug community in the region is not well defined. This study aimed to address this gap with a 2-yr, 9-state survey. Specifically, we characterized the relative abundance, richness, and diversity of taxa in this community, and assessed phenological differences in abundance of herbivorous and predatory stink …

Sublethal Effects Of The Insecticide Pyrifluquinazon On The European Honey Bee (Hymenoptera: Apidae), James M. Wilson, Troy D. Anderson, Thomas P. Kuhar

Department of Entomology: Faculty Publications

Pyrifluquinazon (PQZ) is an Insecticide Resistance Action Committee (IRAC) Group 9 insecticide that has recently been registered for use in the United States for control of soft-bodied sucking insect pests. Although it has been classified as practically nontoxic to honey bees, Apis mellifera L. (Hymenoptera: Apidae), based on acute contact bioassays, additional information on sublethal effects of this insecticide on honey bees is lacking. Using a combination of laboratory assays with video movement tracking software and near-field evaluations of colonies foraging in a high-tunnel experiment, we determined that, when fed PQZ at a concentration of 84 mg active ingredient (ai)/liter …

Will Whitcomb: Entomologist And Old-School Naturalist, John R. Ruberson

Department of Entomology: Faculty Publications

It’s a great pleasure and privilege to reflect on the life and career of Dr. Willard Hall Whitcomb. I tend to agree with Norm Leppla’s assessment that “Dr. Willard Whitcomb, without a doubt, was the most colorful entomological character I have ever known.” I knew Will chiefly by scientific reputation before briefly meeting him in 1988 for the first time. My first significant interactions with Will came in 1994 while I was with the University of Georgia in Tifton, and we tossed ideas back and forth until his death in 2002. However, I never felt that I knew the man …

Wheat Stem Maggot (Diptera: Chloropidae): An Emerging Pest Of Cover Crop To Corn Transition Systems, Gabriela Inveninato Carmona, Jennifer M. Rees, Ronald C. Seymour, Robert J. Wright, Anthony J. Mcmechan

Department of Entomology: Faculty Publications

The wheat stem maggot (Meromyza americana Fitch) (WSM) is a minor pest of wheat, rye, and other grasses. In 2017, growers in Nebraska reported dead center whorls and excessive tillering in early-season cornfields that followed wheat or rye terminated after planting corn. A survey was conducted to evaluate the risk factors for this insect in cover crop to corn transition systems. In each field, management practices and the percentage of injured plants were recorded. Symptomatic corn plants were collected from each field and dissected to determine larval and plant characteristics. In a few cases, small patches of a field …

Ecophysiological Determinants Of Sexual Size Dimorphism: Integrating Growth Trajectories, Environmental Conditions, And Metabolic Rates, Marie-Claire Chelini, John P. Delong, Eileen A. Hebets

Eileen Hebets Publications

Sexual size dimorphism (SSD) often results in dramatic differences in body size between females and males. Despite its ecological importance, little is known about the relationship between developmental, physiological, and energetic mechanisms underlying SSD. We take an integrative approach to understand the relationship between developmental trajectories, metabolism, and environmental conditions resulting in extreme female-biased SSD in the crab spider Mecaphesa celer (Thomisidae). We tested for sexual differences in growth trajectories, as well as in the energetics of growth, hypothesizing that female M. celer have lower metabolic rates than males or higher energy assimilation. We also hypothesized that the environment in …

Differential Spatial Gradients Of Wheat Streak Mosaic Virus Into Winter Wheat From A Central Mite-Virus Source, Abby R. Stilwell, Donald Rundquist, David B. Marx, Gary L. Hein

Department of Entomology: Faculty Publications

The wheat curl mite (WCM), Aceria tosichella Keifer, transmits three potentially devastating viruses to winter wheat. An increased understanding of mite movement and subsequent virus spread through the landscape is necessary to estimate the risk of epidemics by the virus in winter wheat. Owing to the small size of WCMs, their dispersal via wind is hard to monitor; however, the viruses they transmit produce symptoms that can be detected with remote sensing. The objective of this study was to characterize the spatial dispersal of the virus from a central mite-virus source. Virus infection gradients were measured spatially by using aerial …

Advances In Molecular Research On Bed Bugs (Hemiptera: Cimicidae), Sanjay Basnet, Shripat T. Kamble

Department of Entomology: Faculty Publications

With the resurgence and increase in infestations of the common bed bug, Cimex lectularius L. (Hemiptera: Cimicidae), across the world, there has been renewed interest in molecular research on this pest. In this paper, we present current information on the biology, medical importance, management practices, behavior and physiology, and molecular research conducted on bed bugs. The majority of molecular studies are focused towards understanding the molecular mechanism of insecticide resistance. Bed bugs are hematophagous insects with no prior record of vectoring any disease organisms. An improved understanding of how bed bugs lack vector competency may provide information to prevent disease …

Evaluation Of Reference Genes For Real-Time Quantitative Pcr Analysis In Southern Corn Rootworm, Diabrotica Undecimpunctata Howardi (Barber), Saumik Basu, Adriano E. Pereira, Daniele H. Pinheiro, Haichuan Wang, Arnubio Valencia-Jimenez, Blair D. Siegfried, Joe Louis, Xuguo 'Joe' Zhou, Ana Maria Velez

Department of Entomology: Faculty Publications

Quantitative reverse transcription PCR (RT-qPCR) is one of the most efficient, reliable and widely used techniques to quantify gene expression. In this study, we evaluated the performance of six southern corn rootworm, Diabrotica undecimpunctata howardi (Barber), housekeeping genes (HKG), β-actin (Actin), β-tubulin (Tubulin), elongation factor 1 alpha (EF1α), glyceraldehyde-3 phosphate dehydrogenase (GAPDH), 40 S ribosomal protein S9 (RpS9) and ubiquitin-conjugating protein (Ubi), under different experimental conditions such as developmental stage, exposure of neonate and adults to dsRNA, exposure of adults to different temperatures, different 3^rd instar larva …

Nanomaterials For Double-Stranded Rna Delivery, Stuart Lichtenberg

Theses and Dissertations--Plant and Soil Sciences

RNA interference has enormous potential as a potent, specific, and environmentally friendly alternative to small molecule pesticides for crop protection. The use of exogenous double-stranded RNA offers flexibility in targeting and use in crops in which transgenic manipulation is not an option. The combination of RNAi with nanotechnology offers further advantages that are not available with dsRNA alone. In this work, I have evaluated several different combinations of nanomaterials and polymers for use in RNAi-based pest control systems. First, I have characterized the use of chitosan/dsRNA polyplex nanoparticles for gene knockdown using the model nematode Caenorhabditis elegans. Though chitosan/dsRNA …

Do Saproxylic Curculionids Affect The Fitness Of Co-Occurring Cerambycids?, Sheila R. Heath

Dissertations and Theses

Saproxylic insects sometimes coexist in incredibly high numbers under bark and share common resources. Thus, interactions between species are possible and could even explain their coexistence. This study investigates evidence of negative or positive effects of curculionid beetles (Coleoptera: Curculionidae) on cerambycid beetles (Coleoptera: Cerambycidae) that coexisted in dead tree branches in Costa Rica. Co-occurrence analysis and generalized regressions were used to test associations between cerambycid and curculionid species. Three cerambycid species that each co-occurred with a curculionid species were selected to measure fitness. Fitness measures of the cerambycid were compared with abundance of the co-occurring curculionid to assess the …

Investigating The Prevalence Of An Insecticide Resistance Gene In Culex Mosquito Populations Of Central Illinois, Kylee R. Noel

Masters Theses

The globalization of society has led to the spread of the vectors, mosquitoes, and diseases. Each year millions of people are killed by diseases vectored by mosquitoes. For example, West Nile Virus alone killed over 2,000 people in North America in 2017; 8 of those deaths being in Illinois. Two prominent vectors of West Nile Virus are Culex pipiens and Culex restuans; which are difficult to differentiate based upon morphological features. In this study, mosquitoes collected from two Illinois counties, Coles County and Champaign County, were first identified based upon morphology and this identification was then compared to probe-based …

Cold Tolerance, Temperature Mediated Discontinuous Gas Exchange, And Emergence Of The Blue Orchard Mason Bee (Osmia Lignaria), Logan Kral

All Master's Theses

The relationship between low temperatures, emergence and supercooling point of Osmia lignaria were the subject of this study. One hundred sixty-eight bees were subjected to 5 pre-wintering temperature treatments (two constant temperature controls - 22^oC, 14^oC, one of which with and one without a 12h photoperiod, and three 12h:12h thermoperiod treatments – 14:10^oC, 14:5^oC, and 14:0^oC) and were then evaluated in terms of emergence time and post-emergence vigor. An additional 70 bees were tested for metabolic rate and discontinuous gas exchange in response to test temperature conditions. An additional sample …