Entomology Commons^™

Unity In Defence: Honeybee Workers Exhibit Conserved Molecular Responses To Diverse Pathogens, Vincent Doublet, Yvonne Poeschl, Andreas Gogol-Döring, Cédric Alaux, Desiderato Annoscia, Christian Aurori, Seth M. Barribeau, Oscar C. Bedoya-Reina, Mark J.F. Brown, James C. Bull, Michelle L. Flenniken, David A. Galbraith, Elke Genersch, Sebastian Gisder, Ivo Grosse, Holly L. Holt, Dan Hultmark, H. Michael G. Lattorff, Yves Le Conte, Fabio Manfredini, Dino P. Mcmahon, Robin F.A. Moritz, Francesco Nazzi, Elina L. Niño, Katja Nowick, Ronald P. Van Rij, Robert J. Paxton, Christina M. Grozinger

All Peer-Reviewed Publications

Background: Organisms typically face infection by diverse pathogens, and hosts are thought to have developed specific responses to each type of pathogen they encounter. The advent of transcriptomics now makes it possible to test this hypothesis and compare host gene expression responses to multiple pathogens at a genome-wide scale. Here, we performed a meta-analysis of multiple published and new transcriptomes using a newly developed bioinformatics approach that filters genes based on their expression profile across datasets. Thereby, we identified common and unique molecular responses of a model host species, the honey bee (Apis mellifera), to its major pathogens and parasites: …

Hexapod Herald - Vol. 29, No. 2, March 2017

Hexapod Herald and Other Entomology Department Newsletters

Contents: Congratulations - Publications - Blasts from the Past - Grants - Faculty News - This 'n That - Meet an Online Student

Preliminary Study Of Pan Trapping In Longleaf Pine Flatwoods In Central Florida, Usa, A. B. Orfinger, T. B. Cogen, G. R. Irons, K. V. Kabilan, B. Molligoda, A. L. Moody, J. F. Wisdo, K. F. Phillips

Midsouth Entomologist

It is well documented that pan trap color greatly influences arthropod catch composition. Additionally, it has been demonstrated that pan trapping catch varies with habitat. The goal of this study is to present preliminary data on pan trap yield from an imperiled ecosystem marked by high rates of endemicity, longleaf pine flatwoods. Yellow, blue, and white pan traps were evaluated in a section of longleaf pine flatwoods in June 2017 in central Florida, USA. A total of 360 arthropod specimens representing nine taxonomic orders were recovered, with 117 individuals from yellow traps, 125 from blue, and 118 from white. Contrary …

A Role For Intercept Traps In The Ambrosia Beetle (Coleoptera: Curculionidae: Scolytinae) Ipm Strategy At Ornamental Nurseries, C. T. Werle, B. J. Sampson, M. E. Reding

Midsouth Entomologist

Invasive ambrosia beetles (Coleoptera: Curculionidae: Scolytinae) cause significant damage to ornamental nursery tree crops throughout the eastern United States. Depending on surrounding habitat, some nurseries can undergo large influxes of ambrosia beetles from the forest to susceptible nursery stock. Ethanol-baited intercept traps are highly effective as monitoring tools that can capture large numbers of dispersing ambrosia beetles. Beetle trap captures at varying distances within nursery interiors were determined across replicated transects that either included or lacked a row of edge intercept traps. Although nearly 90% of ambrosia beetle captures were from the edge intercept traps, there was no significant difference …

Nebline, March 2017

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Extension’s Agricultural Apps Put Management Tools in Producers’ Hands

Food & Health

Farm & Acreage

Pests & Wildlife

Horticulture

Early Childhood

4-H & Youth

and other extension news and events

2016 Soybean Insect Losses In The Southern Us, F. R. Musser, A. L. Catchot Jr., J. A. Davis, G. M. Lorenz, T. Reed, D. D. Reisig, S. D. Stewart, S. Taylor

Midsouth Entomologist

Estimated soybean insect costs and losses experienced during 2016 were collected and compiled following the 2016 growing season to provide a record of insect pressure and management practices for the year. This survey has been done annually in all participating states since at least 2011, with Mississippi having data back to 2004. The 2016 survey represents 10.8 million soybean acres across the southern United States. Overall, corn earworm was the most expensive pest in soybean followed closely by soybean looper and stink bugs. Total insect management costs rose slightly to $26.03 per acre during 2016. Estimated crop losses to insects …

Temperature-Dependent Phenology Of Plutella Xylostella (Lepidoptera: Plutellidae): Simulation And Visualization Of Current And Future Distributions Along The Eastern Afromontane, Benignus V. Ngowi, Henri E.Z. Tonnang, Evans M. Mwangi, Tino Johansson, Janet Ambale, Paul N. Ndegwa, Sevgan Subramanian

All Peer-Reviewed Publications

There is a scarcity of laboratory and field-based results showing the movement of the diamondback moth (DBM) Plutella xylostella (L.) across a spatial scale. We studied the population growth of the diamondback moth (DBM) Plutella xylostella (L.) under six constant temperatures, to understand and predict population changes along altitudinal gradients and under climate change scenarios. Non-linear functions were fitted to continuously model DBM development, mortality, longevity and oviposition. We compiled the best-fitted functions for each life stage to yield a phenology model, which we stochastically simulated to estimate the life table parameters. Three temperature-dependent indices (establishment, generation and activity) were …

Green Tea Proanthocyanidins Cause Impairment Of Hormone-Regulated Larval Development And Reproductive Fitness Via Repression Of Juvenile Hormone Acid Methyltransferase, Insulin-Like Peptide And Cytochrome P450 Genes In Anopheles Gambiae Sensu Stricto, Jackson M. Muema, Steven G. Nyanjom, James M. Mutunga, Sospeter N. Njeru, Joel L. Bargul

All Peer-Reviewed Publications

Successful optimization of plant-derived compounds into control of nuisance insects would benefit from scientifically validated targets. However, the close association between the genotypic responses and physiological toxicity effects mediated by these compounds remains underexplored. In this study, we evaluated the sublethal dose effects of proanthocyanidins (PAs) sourced from green tea (Camellia sinensis) on life history traits of Anopheles gambiae (sensu stricto) mosquitoes with an aim to unravel the probable molecular targets. Based on the induced phenotypic effects, genes selected for study targeted juvenile hormone (JH) biosynthesis, signal transduction, oxidative stress response and xenobiotic detoxification in addition to vitellogenesis in females. …

The Role Of Hyalomma Truncatum On The Dynamics Of Rift Valley Fever: Insights From A Mathematical Epidemic Model, Sansao A. Pedro, Shirley Abelman, Henri E.Z. Tonnang

All Peer-Reviewed Publications

To date, our knowledge of Rift Valley fever (RVF) disease spread and maintenance is still limited, as flooding, humid weather and presence of biting insects such as mosquitoes, have not completely explained RVF outbreaks. We propose a model that includes livestock, mosquitoes and ticks compartments structured according to their questing and feeding behaviour in order to study the possible role of ticks on the dynamics of RVF. To quantify disease transmission at the initial stage of the epidemic, we derive an explicit formula of the basic reproductive number, R0. Using the concept of Metzler matrix, we state necessary conditions for …

Dietary Risk Assessment Of V-Atpase A Dsrnas On Monarch Butterfly Larvae, Huipeng Pan, Xiaowei Yang, Keith Bidne, Richard L. Hellmich, Blair D. Siegfried, Xuguo Zhou

Entomology Faculty Publications

By suppressing the expression of genes with essential biological functions, in plantaRNAi can negatively affect the development and survival of target pests. As a part of a concerted effort to assess the risks of RNAi transgenic crops on non-target organisms, we developed an in vivo toxicity assay to examine the impacts of ingested dsRNAs incurred to the monarch butterfly, Danaus plexippus (L.), an iconic eco-indicator in North America. To create the worst case scenario, the full-length v-ATPase A cDNAs from the target pest, western corn rootworm, Diabrotica virgifera virgifera, and the non-target D. plexippus were respectively cloned. A …

Dietary Risk Assessment Of V-Atpase A Dsrnas On Monarch Butterfly Larvae, Huipeng Pan, Xiaowei Yang, Keith Bidne, Richard L. Hellmich, Blair Siegfried, Xuguo Zhou

Department of Entomology: Faculty Publications

By suppressing the expression of genes with essential biological functions, in planta RNAi can negatively affect the development and survival of target pests. As a part of a concerted effort to assess the risks of RNAi transgenic crops on non-target organisms, we developed an in vivo toxicity assay to examine the impacts of ingested dsRNAs incurred to the monarch butterfly, Danaus plexippus (L.), an iconic eco-indicator in North America. To create the worst case scenario, the full-length v-ATPase A cDNAs from the target pest, western corn rootworm, Diabrotica virgifera virgifera, and the non-target D. plexippus were respectively cloned. A 400 …

Distribution And Abundance Of Key Vectors Of Rift Valley Fever And Other Arboviruses In Two Ecologically Distinct Counties In Kenya, Rosemary Sang, Samwel Arum, Edith Chepkorir, Gladys Mosomtai, Caroline Tigoi, Faith Sigei, Olivia Wesula Lwande, Tobias Landmann, Hippolyte Affognon, Clas Ahlm, Magnus Evander

All Peer-Reviewed Publications

Background: Rift Valley fever (RVF) is a mosquito-borne viral zoonosis of ruminants and humans that causes outbreaks in Africa and the Arabian Peninsula with significant public health and economic consequences. Humans become infected through mosquito bites and contact with infected livestock. The virus is maintained between outbreaks through vertically infected eggs of the primary vectors of Aedes species which emerge following rains with extensive flooding. Infected female mosquitoes initiate transmission among nearby animals, which amplifies virus, thereby infecting more mosquitoes and moving the virus beyond the initial point of emergence. With each successive outbreak, RVF has been found to expand …

The One Health Approach To Identify Knowledge, Attitudes And Practices That Affect Community Involvement In The Control Of Rift Valley Fever Outbreaks, Osama Ahmed Hassan, Hippolyte Affognon, Joacim Rocklöv, Peter Mburu, Rosemary Sang, Clas Ahlm, Magnus Evander

All Peer-Reviewed Publications

Rift Valley fever (RVF) is a viral mosquito-borne disease with the potential for global expansion, causes hemorrhagic fever, and has a high case fatality rate in young animals and in humans. Using a cross-sectional community-based study design, we investigated the knowledge, attitudes and practices of people living in small village in Sudan with respect to RVF outbreaks. A special One Health questionnaire was developed to compile data from 235 heads of household concerning their knowledge, attitudes, and practices with regard to controlling RVF. Although the 2007 RVF outbreak in Sudan had negatively affected the participants’ food availability and livestock income, …

Resistance Monitoring Of Oebalus Pugnax F., Rice Stink Bug, Against Select Insecticides, Audra Lee Cross

Student Theses and Dissertations

Rice stink bug, Oebalus pugnax F. is a pest of rice that impacts quality of grain. Pyrethroid insecticides are the main control method. However, there is concern that pyrethroid insecticides are not providing adequate control, due to development of insecticide resistance. With this in mind, efforts were focused on monitoring rice stink bug resistance to λ-cyhalothrin, a common pyrethroid, and obtaining baseline data for a new chemistry of insecticides, a neonicotinoid (dinotefuran), for rice stink bug. For the purposes of the research presented here the adult vial test was used for monitoring λ-cyhalothrin resistance and a modified adult vial test …

Phylogenetic Analysis And Systematics Of The Acrapex Unicolora Hampson Species Complex (Lepidoptera, Noctuidae, Noctuinae, Apameini), With The Description Of Five New Species From The Afrotropics, Bruno Le Ru, Claire Capdevielle-Dulac, Boaz K. Musyoka, Beatrice Pallangyo, Mohamedi Njaku, Onésime Mubenga, Gilson Chipabika, Rose Ndemah, Grégoire Bani, Richard Molo, George Ong’Amo, Gael J. Kergoat

All Peer-Reviewed Publications

Ten morphologically similar species of Acrapex Hampson, 1891 (Lepidoptera, Noctuidae, Noctuinae, Apameini) from Central and Eastern Africa are reviewed, including five new species: Acrapex kafula le Ru sp. nov., A. kavumba le Ru sp. nov., A. kiakouama le Ru sp. nov., A. miscantha le Ru sp. nov. and A. simillima le Ru sp. nov. Evidence is provided to transfer the monotypic genus Poecopa Bowden, 1956 to the genus Acrapex. Host plants of five species are recorded, some of them for the first time. Acrapex kavumba sp. nov., A. miscantha sp. nov. and A. simillima sp. nov. were found on one …

Nebline, February 2017

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: 3rd Graders Learn About Life Cycle By Hatching Eggs in Classrooms

Food & Health

Farm & Acreage

Pests & Wildlife

Horticulture

Early Childhood

4-H & Youth

and other extension news and events

Teaching Morphological Species Identification To Forensic Science Students: Advantages, Problems And Results, Gregory Nigoghosian, Lauren Weidner, Ludmila Nunes, Trevor Stamper

IMPACT Presentations

Poster presented at the 69th American Academy of Forensic Science Annual Meeting, New Orleans, LA, USA. The poster discusses findings of a study of how students identify morphological species.

Diversity Of Lepidopteran Stemborers And Their Parasitoids On Maize And Wild Host Plants In The Rain Forest Of Kisangani, Dr Congo, Onésime Mubenga Kankonda, Benjamin Dudu Akaibe, George Otieno Ong’Amo, Bruno Pierre Le Ru

All Peer-Reviewed Publications

Lepidopteran stemborers are considered as the most important insect pests of cereal crops in Sub-Saharan Africa. However, limited information exists on stemborers and their parasitoids in the rain forest of the DR Congo. In order to enhance the understanding of the pest ecology, sampling in both cultivated and wild habitats has been recommended as a bottom line to any action aiming at the design of pest management measures. Following this recommendation, a survey was carried out in the rain forest of the DR Congo on maize and wild host plants. We collected eighteen stemborer and one earborer species belonging to …

At Lunch With A Killer: The Effect Of Weaver Ants On Host-Parasitoid Interactions On Mango, Valentina Migani, Sunday Ekesi, Katharina Merkel, Thomas Hoffmeister

All Peer-Reviewed Publications

Predator-prey interactions can affect the behaviour of the species involved, with consequences for population distribution and competitive interactions. Under predation pressure, potential prey may adopt evasive strategies. These responses can be costly and could impact population growth. As some prey species may be more affected than others, predation pressure could also alter the dynamics among species within communities. In field cages and small observation cages, we studied the interactions between a generalist predator, the African weaver ant, Oecophylla longinoda, two species of fruit flies that are primary pests of mango fruits, Ceratitis cosyra and Bactrocera dorsalis, and their two exotic …

Selection Of Fungal Isolates For Virulence Against Three Aphid Pest Species Of Crucifers And Okra, W. Bayissa, S. Ekesi, S. A. Mohamed, G. P. Kaaya, J. M. Wagacha, R. Hanna, N. K. Maniania

All Peer-Reviewed Publications

Aphids are regarded as important pest problems of vegetable crops worldwide. Most vegetable growers in sub-Saharan Africa heavily rely on synthetic chemical insecticides for aphids’ control. Fungus-based biopesticides are being considered as alternatives to chemical insecticides. This study evaluates virulence of five isolates of Metarhizium anisopliae and three of Beauveria bassiana against Brevicoryne brassicae, Lipaphis pseudobrassicae, and Aphis gossypii, and their thermotolerance and conidial yield as a prerequisite for strain selection. The study also evaluates performance of the best isolate in screenhouse experiment against target aphid species. Three isolates of M. anisopliae ICIPE30, ICIPE62, and ICIPE69 outperformed the others, causing …

Clarifying The Source Of Conicofrontia Sesamoides Hampson (Lepidoptera: Noctuidae) Population In South African Sugarcane Using Morphological Identification And Mitochondrial Dna Analysis, Y. Assefa, M. Goftishu, C. Capdevielle-Dulac, B. Le Ru

All Peer-Reviewed Publications

Valid identification of a novel pest species and clarifying its origin are the primary steps in understanding population structure and development of biocontrol programs. In this study geographical populations of Conicofrontia sesamoides Hampson (Lepidoptera: Noctuidae) collected during surveys conducted in the years 2009, 2014 and 2015 were morphologically identified and their genetic diversity was analysed by using sequences of the mitochondrial cytochrome-c oxidase I (COI) gene in an attempt to examine host plant, or/and altitude associated differences among populations and determine the source of the newly recorded population of this species in the South African sugarcane. The C. sesamoides specimens …

Can Toxicants Used Against Cotton Mealybug Phenacoccus Solenopsis Be Compatible With An Encyrtid Parasitoid Aenasius Bambawalei Under Laboratory Conditions?, Hayat Badshah, Farman Ullah, Paul Andre Calatayud, Hidayat Ullah, Bashir Ahmad

All Peer-Reviewed Publications

The cotton mealybug, Phenacoccus solenopsis Tinsley (Sternorrhyncha: Pseudococcidae) is a serious pest of various cultivated plants in Pakistan. Recent reports show that the parasitoid Aenasius bambawalei Hayat (Hymenoptera: Encyrtidae) is a good biocontrol agent of the pest. Compatibleness is important in any IPM programme, and the insecticide used must have little or no effects on the biological control agent. This study investigated the compatibility of neem treatments and a commercial insecticide, imidacloprid on A. bambawalei. Bioassays were laid out in a completely randomized design (CRD) under laboratory conditions. Results showed that the adult stage of the parasitoid was more susceptible …

Transcriptomic Response Of Wolf Spider, Pardosa Pseudoannulata, To Transgenic Rice Expressing Bacillus Thuringiensis Cry1ab Protein, Juan Wang, Yuande Peng, Kaifu Xiao, Baoyang Wei, Jilin Hu, Zhi Wang, Qisheng Song, Xuguo Zhou

Entomology Faculty Publications

Background: Bacillum thuringiensis (Bt) toxin produced in Cry1-expressing genetically modified rice (Bt rice) is highly effective to control lepidopteran pests, which reduces the needs for synthetic insecticides. Non-target organisms can be exposed to Bt toxins through direct feeding or trophic interactions in the field. The wolf spider Pardosa pseudoannulata, one of the dominant predators in South China, plays a crucial role in the rice agroecosystem. In this study, we investigated transcriptome responses of the 5th instar spiders fed on preys maintained on Bt- and non-Bt rice.

Results: Comparative transcriptome analysis resulted in 136 differentially expressed genes (DEGs) …

The Mayfly Newsletter, Donna J. Giberson

The Mayfly Newsletter

The Mayfly Newsletter is the official newsletter of the Permanent Committee of the International Conferences on Ephemeroptera.

Genome Editing Of Wnt-1, A Gene Associated With Segmentation, Via Crispr/Cas9 In The Pine Caterpillar Moth, Dendrolimus Punctatus, Huihui Liu, Qun Liu, Xuguo Zhou, Yongping Huang, Zhen Zhang

Entomology Faculty Publications

The pine caterpillar moth, Dendrolimus punctatus, is a devastating forest pest. Genetic manipulation of this insect pest is limited due to the lack of genomic and functional genomic toolsets. Recently, CRISPR/Cas9 technology has been demonstrated to be a promising approach to modify the genome. To investigate gene functions during the embryogenesis, we introduced CRISPR/Cas9 system in D. punctatus to precisely and effectively manipulate gene expressions inmutant embryos. Compared to controls, knocking out of DpWnt-1, a gene well known for its role in the early body planning, led to high embryonic mortality. Among these mutants, 32.9% of the embryos …

Integrating Kaolin Clay For Ambrosia Beetle Management In Ornamental Crops Of Eastern Redbud, Christopher T. Werle, Karla M. Addesso, Blair J. Sampson, Jason B. Oliver, John J. Adamczyk

Agricultural and Environmental Sciences Faculty Research

Invasive ambrosia beetles (Coleoptera: Curculionidae) are an important pest problem at ornamental tree nurseries. Available chemical treatments are not completely effective and, due to the length of the beetle dispersal period and insecticide breakdown, repeated treatments can become costly in terms of application expense and nontarget impacts. Additional options are needed to reduce application frequency and to provide an acceptable level of crop protection. Four treatments were tested using ethanol-injected eastern redbud trees at research sites in Mississippi (MS) and Tennessee (TN) over 2 years (2014–15), with the number of new ambrosia beetle galleries compared over time on 1) nontreated …

Hexapod Herald - Vol. 29, No. 1, January 2017

Hexapod Herald and Other Entomology Department Newsletters

Content: Welcome - Faculty News - Grants - Congratulations - Student News - Blast from the Past - Publications - Condolences (Dr. Kenneth P. Pruess)

Nebline, January 2017

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Extension 2016 Highlights

Food & Health

Farm & Acreage

Horticulture

Pests & Wildlife

4-H & Youth

Early Childhood

and other extension news and events

Bioassessment Of Four Karst Springs At Hobbs State Park – Conservation Area With A Focus On Diving Beetle (Dytiscidae: Hydroporinae) Species Of Concern, Scott Longing, L. A. Mack, Brian E. Haggard

Journal of the Arkansas Academy of Science

Four springs were surveyed at Hobbs State Park-Conservation Area to provide an initial bioassessment and to determine occurrences of two endemic predaceous diving beetles of concern, Heterosternuta sulphuria and Sanfilippodytes sp. Habitat in the four spring runs were dominated by bedrock and gravel substrate with heavy accumulations of leaf litter. Thirty-three taxa representing 11 orders were collected from the four springs. Non-insect taxa included Oligochaeta, Physidae, and Isopoda, and predominant insect orders included Ephemeroptera, Coleoptera, Diptera, and Trichoptera. The total number of taxa across springs ranged from seven to 19, with total abundances ranging from 39 to 86 individuals. No …

Bumpers College Students In Action, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.