Entomology Commons^™

The Uas-Gal4 System In D. Melanogaster: An Insight Into The Influence Of Micrornas On The Developmental Pathways Of The Wing, Emily R. Wilson

Honors Theses

By examining genetic pathways in D. melanogaster, a better understanding of the homologous regulatory mechanisms in humans can be utilized to further enhance knowledge of the roles of microRNA within development. This study utilizes the UAS-Gal4 system in order to produce a mutant phenotype capable of being visually studied and analyzed, focusing on the developmental pathway of the wing in D. melanogaster. Dissections of the wandering third instar larvae yielded wing disc tissue expressing the downregulation of loquacious and CG17386.

Capturing The Phylogeny Of Holometabola With Mitochondrial Genome Data And Bayesian Site-Heterogeneous Mixture Models, Fan Song, Hu Li, Pei Jiang, Xuguo Zhou, Jinpeng Liu, Changhai Sun, Alfried P. Vogler, Wanzhi Cai

Entomology Faculty Publications

After decades of debate, a mostly satisfactory resolution of relationships among the 11 recognized holometabolan orders of insects has been reached based on nuclear genes, resolving one of the most substantial branches of the tree-of-life, but the relationships are still not well established with mitochondrial genome data. The main reasons have been the absence of sufficient data in several orders and lack of appropriate phylogenetic methods that avoid the systematic errors from compositional and mutational biases in insect mitochondrial genomes. In this study, we assembled the richest taxon sampling of Holometabola to date (199 species in 11 orders), and analyzed …

Emergence Phenology And Nematode Associates Of The Woodwasp, Sirex Nigricornis F. (Hymenoptera: Siricidae), In Arkansas And Mississippi Forests, Jessica Ann Hartshorn

Graduate Theses and Dissertations

Sirex nigricornis F. (Hymenoptera: Siricidae) is a native woodwasp in eastern North America that inhabits dead and dying pine trees during its univoltine development. Sirex noctilio is native to Eurasia and North Africa and was discovered in the northeastern United States in 2004 after a century of accidental introductions across the Southern Hemisphere. Since then, it has spread to seven states and southern Ontario. There is concern about its potential to negatively affect the multi-billion dollar pine timber industry of the southeastern United States. Effective monitoring tools are necessary to track the spread and establishment of S. noctilio. Deladenus siricidicola …

Influence Of Host-Plant Surface Chemicals On The Oviposition Of The Cereal Stemborer Busseola Fusca, Gerald Juma, Gilles Clément, Peter Ahuya, Ahmed Hassanali, Sylvie Derridj, Cyrile Gaertner, Romain Linard, Bruno Le Ru, Brigitte Frérot, Paul André Calatayud

All Peer-Reviewed Publications

The chemical composition of plant surfaces plays a role in selection of host plants by herbivorous insects. Once the insect reaches the plant, these cues determine host acceptance. Laboratory studies have shown that the stem borer Busseola fusca (Lepidoptera: Noctuidae), an important pest of sorghum and maize in sub-Saharan Africa, is able to differentiate between host and non-host plant species. However, no information is available on the cues used by this insect to seek and accept the host plant. Thus, the role of surface phytochemical stimuli on host selection and oviposition by B. fusca was studied in the laboratory using …

Integrative Taxonomy Of North American Torrent Mites (Parasitengona: Torrenticolidae: Torrenticola), Jon Ray Fisher

Graduate Theses and Dissertations

Mites are notorious for being under-studied and incomplete knowledge of distribution, life-history, and morphology are commonplace. They typically gain attention through the relatively few pest species that plague our crops, pets, or bodies. Despite representing a successful radiation with an estimated 3–5 million species, relatively few mite lineages attract research attention from non-acarologists. However, the largest radiation of all mites—Parasitengona—has potential to bridge the gap between specialists and non-specialists. Parasitengona are the butterflies of the mite world. Obvious are the bright red mites searching for pollen on concrete, or the large, furry velvet mites coming out to mate after spring …

Influence Of Fatty Acids And Their Derivatives On Aphid Resistance In Arabidopsis And Tomato, Jiamei Li

Graduate Theses and Dissertations

Fatty acid desaturases (FADs) are enzymes that act in the chloroplast or the endoplasmic reticulum (ER) to incorporate double bonds into the acyl chains of fatty acids, and recent evidence indicates that at least one of these enzymes, FAD7, also influences plant resistance to aphids. FAD7 is an enzyme in the chloroplast that is found throughout the plant kingdom and that desaturates 16- and 18-carbon fatty acids (FAs) with two double bonds (dienoic acids) to generate FAs with three double bonds (trienoic acids). In tomato (Solanum lycopersicum) and the model plant Arabidopsis thaliana, mutants with impaired FAD7 function are more …

Biology, Ecology, And Management Of Nonnative Ambrosia Beetles (Coleoptera: Curculionidae: Scolytinae) In Ornamental Plant Nurseries, Christopher M. Ranger, Michael E. Reding, Peter B. Schultz, Jason B. Oliver, Steve D. Frank, Karla M. Addesso, Juang Hong Chong, Blair Sampson, Christopher Werle, Stanton Gill, Charles Krause

Agricultural and Environmental Sciences Faculty Research

Xylosandrus germanus (Blandford) and Xylosandrus crassiusculus (Motschulsky) (Coleoptera: Curculionidae: Scolytinae) are two of the most damaging nonnative ambrosia beetle pests in ornamental plant nurseries. Adult females tunnel into the stems and branches of host plants to create galleries with brood chambers. Hosts are infected with symbiotic Ambrosiella spp. fungi that serve as food for the larvae and adults. Plants can also become infected with secondary opportunistic pathogens, including Fusarium spp. Both X. germanus and X. crassiusculus have broad host ranges, and infestations can result in “toothpicks” of extruded chewed wood and sap flow associated with gallery entrances, canopy dieback, stem …

Height Perception Of Bed Bugs, Cimex Lectularius, Loren D. Hall

Capstone Research Projects

It has been demonstrated that there is no significant difference in Bed bug preference for colored columns of two heights and that Bed bugs tend to hide in harborages when not stimulated. More information, however, is still needed on their visual perception and general behavior. The following study investigated height perception of Bed bugs by determining the total number of bugs that migrated toward Bed bug interceptors representing different elevations over several trials. A significant difference was found between the number of bugs that migrated toward the control and experimental elevations (3”, 6”, 9”) and the number that migrated toward …

Nontarget Effects Of Orchard Pesticides On Natural Enemies: Lessons From The Field And Laboratory, Elizabeth H. Beers, Nicholas J. Mills, Peter W. Shearer, David R. Horton, Eugene R. Milickzy, Kaushalya G. Amarasekare, Lessando M. Gontijo

Agricultural and Environmental Sciences Faculty Research

The nontarget effects of insecticide programs used to control codling moth, Cydia pomonella L. (Lepidoptera: Tortricidae), were studied in large-plot field trials in apples, pears, and walnuts in the western United States. We assessed the health of the natural enemy community by sampling the abundance of natural enemies and by monitoring for outbreaks of secondary pests. The insecticides used in the field tests overlapped those tested in laboratory bioassays. Using these parallel lab and field studies, we examined two hypotheses: 1) pesticides found to have negative effects on natural enemy fitness in laboratory bioassays will predict reductions in natural enemy …

Aphid Performance Changes With Plant Defense Mediated By Cucumber Mosaic Virus Titer, Xiaobin Shi, Yang Gao, Shuo Yan, Xin Tang, Xuguo Zhou, Deyong Zhang, Yong Liu

Entomology Faculty Publications

Background: Cucumber mosaic virus (CMV) causes appreciable losses in vegetables, ornamentals and agricultural crops. The green peach aphid, Myzus persicae Sulzer (Aphididae) is one of the most efficient vectors for CMV. The transmission ecology of aphid-vectored CMV has been well investigated. However, the detailed description of the dynamic change in the plant-CMV-aphid interaction associated with plant defense and virus epidemics is not well known.

Results: In this report, we investigated the relationship of virus titer with plant defense of salicylic acid (SA) and jasmonic acid (JA) during the different infection time and their interaction with aphids in CMV-infected tobacco plants. …

Managing Drought Stress In California Agricultural Systems, Gregory D. Brittain Jr.

Doctor of Plant Health Program: Dissertations and Student Research

California is currently facing a historic drought, and this has led many farmers in the state to severely cut back on irrigation. Optimal use of water for irrigation requires a comprehensive understanding of how plants respond physiologically to water stress (Chapter 1). By monitoring water requirements in crops and managing irrigation to meet those requirements, growers can significantly reduce water use (Chapter 2). This can be done through improving application efficiency of irrigation technology as well as increasing the water use efficiency of the crops themselves. Deficit irrigation practices can be used to manipulate the physiology of water use in …

Ecological Risks Of The Conventional Insecticide/Fungicide Seed Treatment Mixture Of Thiamethoxam And Mefenoxam In Soybean On Beneficial Insects, Carolina Camargo

Department of Entomology: Dissertations, Theses, and Student Research

The impact of neonicotinoid seed treatments on beneficial insects has been a controversial topic during the last years. While neonicotinoids are usually used as mixtures with systemic fungicides, few studies have examined the impact of the mixtures on beneficial insects. Pesticide mixtures can have synergistic, additive, or antagonistic effects on the toxicity of neonicotinoids on non-target species.

Thiamethoxam with mefenoxam is the most used neonicotinoid insecticide/fungicide mixture applied to soybean. Based on the systemic nature of thiamethoxam and mefenoxam, residues of this insecticide/fungicide mixture can be present in soybean vegetative and floral tissue with potential impacts to beneficial insects. This …

Export Of Insect Ecosystem Services From Hayed Native Prairie, Wayne J. Ohnesorg

Department of Entomology: Dissertations, Theses, and Student Research

Natural areas, such as prairie, have the potential to provide the benefits of pest suppression and pollination for agricultural production. In Nebraska, prairie is often used for hay production. The impact of prairie hay production management practices on beneficial arthropods is not well understood. Four prairie hay meadows adjacent to row crop fields were selected in northeast Nebraska. The goal was to assess the impact of haying them on beneficial arthropods and the movement of natural enemies into adjacent crop fields. Three management practices were evaluated for hay harvested from prairie meadows one cut per growing season, two cuts per …

Arthropods Infesting Small Mammals (Insectivora And Rodentia) Near Cedar Point Biological Station In Southwestern Nebraska, Lindsey Howell, Katelyn Jelden, S. Elizabeth Rácz, Scott L. Gardner, Donald D. Gettinger

Insecta Mundi

Cedar Point Biological Station (CPBS) is located in the mixed grass prairie of the central Great Plains, at the transition between the subregions known as the “tall grass” and “short grass” prairies. Adding to the habitat diversity, there are wetlands and riparian habitats associated with the North Platte River and the edge of the Sandhills region of north central Nebraska. This concurrence of habitats supports a diverse small mammal community. The purpose of this paper is to assemble all published information on ectoparasites associated with small mammals (Insectivora, Rodentia) of southwestern Nebraska, and to report the results of an intensive …

North American Entomophagy, Joohi Schrader, Dennis G. A. B Oonincx, Maria Pontes Ferreira

Nutrition and Food Science Faculty Research Publications

Eating insects is not a common Northern American practice today. However, in the past a variety of insect species was consumed in Northern America (north of Mexico including Greenland). The aim of this literature review is to provide an historical overview of North American entomophagy based upon both peer and non-peer reviewed sources on this topic. Regional differences in insect consumption and reasons for being underreported are discussed. We show that North American natives, and in certain cases colonists, collected and consumed a large variety of edible insects. These are categorized per order and where available, information on how these …

Phylogeny Of The Oniticellini And Onthophagini Dung Beetles (Scarabaeidae, Scarabaeinae) From Morphological Evidence, T. Keith Philips

Biology Faculty Publications

A phylogenetic study was conducted to hypothesize relationships of most of the genera of the Oniticellini and Onthophagini for the first time using morphological characters from a diverse array of external and internal sclerites. The monophyly and sister relationship of both tribes was found using Bayesian and parsimony analyses with heavily to moderately weighted data. An alternative hypothesis based on parsimony analyses of unweighted or slightly weighted data show a paraphyletic Oniticellini without the Onthophagini, although recognition of the subtribe Helictopleurina as a tribe would eliminate non-monophyly.

Of the three Oniticellini subtribes, the Helictopleurina and Drepanocerina are monophyletic. There is …

Uncertainty And Sensitivity Analysis Of A Rift Valley Fever Model, S. A. Pedro, H. E.Z. Tonnang, S. Abelman

All Peer-Reviewed Publications

Rift Valley fever (RVF) is a vector-borne viral disease with pronounced health and economic impacts on domestic animals and humans in much of sub-Saharan Africa. Using techniques of uncertainty and sensitivity analysis (both local derivatives and sampling-based methods) of a mathematical model for RVF transmission in livestock by two population of mosquitoes (Aedes and Culex). We systematically investigate the relative importance of each model parameter for both disease epidemic and endemic activities. The relationship between vertical transmission and basic reproduction number reveals that during epidemic activities vertical transmission accelerates the course of the outbreak as it increases the size of …

Permian Scorpions From The Petrified Forest Of Chemnitz, Germany, Jason A. Dunlop, David A. Legg, Paul L. Selden, Victor Fet, Joerg W. Schneider, Ronny Rößler

Biological Sciences Faculty Research

Background: Paleozoic scorpions (Arachnida: Scorpiones) have been widely documented from the Carboniferous Period; which hosts a remarkable assemblage of more than sixty species including both putative stem- and crown-group fossils. By contrast the succeeding Permian Period is almost completely devoid of records, which are currently restricted to a trace fossil from the early Permian of New Mexico, USA and some limb fragments from the late Permian of the Vologda Region, Russia.

Results: ?Opsieobuthus tungeri sp. nov. from the Petrified Forest of Chemnitz, Germany represents the first complete body fossils of scorpions from the Permian. Explosive volcanism preserved these remarkable …

Host-Parasite Associations Of Small Mammal Communities And Implications For The Spread Of Lyme Disease, Matthew J. Buchholz

Masters Theses & Specialist Projects

Many zoonotic pathogens of concern to human and veterinary health are maintained in the environment within small mammal reservoirs and vectored to new hosts by ectoparasitic arthropods. While the ecological relationships among small mammals, ectoparasites, and disease-causing symbiotic microorganisms are important to these dynamics, little is known about them across much of North America. The sylvatic cycle of Borrelia burgdorferi, the etiologic agent of Lyme disease, is of particular interest because Lyme disease is the most common vector-borne disease of humans in the United States. However, cases of Lyme disease are primarily confined to the northeastern and Midwestern United States, …

Effect Of Varied Disturbance Types On Dorylus (Anomma) Molestus Defensive Behavior In The West Usumbara Mountains, Tanzania., Ryan Mahar

Independent Study Project (ISP) Collection

The purpose of this study was to observe novel nest characteristics of Dorylus molestus (Siafu), as well as their defensive behavior in response to varied disturbance methods. The number of defensive soldiers at a specific section of a column was counted before and after each agitation. Varied agitation methods were used on columns without larvae presence to test how soldiers responded to different disturbance types. Disturbances on columns with larvae presence were compared to those on columns without larvae. Nest distribution, behavior, composition, and size were observed objectively. Mechanical disturbance was found to incite the greatest increase in defensive soldier …

Advances In Solid Phase Microextraction For The Analysis Of Volatile Compounds In Explosives, Tire Treatments, And Entomological Specimens, William D. Kranz

Open Access Dissertations

Solid phase microextraction is a powerful and versatile technique, well-suited to the analysis of numerous samples of forensic interest. The exceptional sensitivity of the SPME platform, combined with its adaptability to traditional GC-MS systems and its ability to extract samples with minimal work-up, make it appropriate to applications in forensic laboratories.

In a series of research projects, solid phase microextraction was employed for the analysis of explosives, commercial tire treatments, and entomological specimens. In the first project, the volatile organic compounds emanating from two brands of pseudo-explosive training aids for use in detector dog imprinting were determined by SPME-GC-MS, and …

Nebline, April 2016

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Climate Impacts on Cropping Systems in Eastern Nebraska

Food & Health

Family Living

Farm & Acreage

Horticulture

Pests & Wildlife

4-H & Youth

Extension Calendar

and other extension news and events

Special Pullout Section: Weed Awareness

Tolerance As A Novel Mechanism Of Hessian Fly Control On Wheat, Kirsten E. Roe

Open Access Theses

The effects of Hessian fly (Mayetiola destructor Say) infestation on the putative tolerant wheat line Pioneer ®brand variety 25R78 were investigated at the seedling stage. Measurements, including leaf and tiller number, leaf growth rate, and total leaf lengths were recorded for two time intervals, 16 and 32 days post infestation (dpi). At 16 dpi, total leaf length changes and leaf growth rates were significantly lower for infested tolerant plants versus uninfested plants. No permanent growth effects occurred in the 32-day set. There were no significant differences in change in leaf length and leaf growth rate in infested tolerant plants …

Reclaiming Lost Territory: The Response Of Owyhee Harvester Ants To Forager Intrusions By Neighboring Colonies, Brett D. Howell

McNair Scholars Research Journal

Neighboring colonies of the Owyhee harvester ant, Pogonomyrmex salinus, often share non-overlapping foraging boundaries in the areas between their nests. We found that interactions between neighbors along these foraging boundaries were infrequent but peaceful, and usually resulted in one or both individuals becoming agitated and scurrying away in opposite directions. Interactions between neighbors were necessary to maintain the foraging ranges of their respective colonies. An exclusion experiment showed that when one colony of a pair situated 5-7 m apart was denied access to its foraging range, individuals from the other colony would usually (i.e., in 7 out of 10 cases) …

Nestmate Recognition In Odorous House Ants (Tapinoma Sessile): Effects Of Social Plasticity, Urbanization, And Laboratory Maintenance, Timothy J. Luttermoser

Open Access Theses

Odorous house ants (Tapinoma sessile) are a widespread North American ant species and common nuisance pest. In addition to their pest status, odorous house ants are of interest as a model system for understanding the factors that lead to variable queen number and nesting strategy across ants, as well as possible insight into common traits of exotic invasive (or “tramp”) ant species. While T. sessile is native to North America, in urban environments it forms large supercolonies with many queens and nest sites connected by trails, similar to a variety of exotic invasive ants, most of which are …

Section Abstracts: Entomology

Virginia Journal of Science

Abstracts of the Entomology Section for the 94th Annual Virginia Academy of Science Meeting, May 18-20, 2016, at University of Mary Washington, Fredericksburg, VA.

Key To The Species Of Megarhyssa (Hymenoptera, Ichneumonidae, Rhyssinae) In America, North Of Mexico, Victoria G. Pook, Michael J. Sharkey, David B. Wahl

Entomology Faculty Publications

A dichotomous and an interactive key to the species of Megarhyssa (Hymenoptera: Ichneumonidae) in America, north of Mexico are presented. A diagnosis accompanied by images is provided for male and female wasps of each of the four species, Megarhyssa atrata, Megarhyssa greenei, Megarhyssa macrurus and Megarhyssa nortoni.

Dispersal And Sampling Of The Wheat Stem Sawfly, Cephus Cintus Norton, (Hymenoptera: Cephidae), Christopher Mccullough

Department of Entomology: Dissertations, Theses, and Student Research

The wheat stem sawfly, Cephus cinctus Norton (Hymenoptera: Cephidae), is a serious insect pest of wheat, Triticum aestivum L., in the northern central Great Plains. The sawfly has been a pest of wheat in Montana, North Dakota, and Canada since the early 20^th century. It was first detected in Nebraska winter wheat in the early 1990s, in Scotts Bluff County. The sawfly has since spread throughout the Nebraska Panhandle region and become a pest of serious concern. To gain a better understanding of the sawfly in Nebraska, investigations on the emergence, dispersal, and sampling of the sawfly were conducted. …

Cry1f Resistance Among Lepidopteran Pests: A Model For Improved Resistance Management?, Ana María Vélez, Neetha Nanoth Vellichirammal, Juan Luis Jurat-Fuentes, Blair Siegfried

Department of Entomology: Faculty Publications

The Cry1Fa protein from the bacterium Bacillus thuringiensis (Bt) is known for its potential to control lepidopteran pests, especially through transgenic expression in maize and cotton. The maize event TC1507 expressing the cry1Fa toxin gene became commercially available in the United States in 2003 for the management of key lepidopteran pests including the European corn borer, Ostrinia nubilalis, and the fall armyworm, Spodoptera frugiperda. A high-dose/refuge strategy has been widely adopted to delay evolution of resistance to event TC1507 and other transgenic Bt crops. Efficacy of this strategy depends on the crops expressing a high dose of the …

Effects Of Environmental Factors On The Abundance Of Blacklegged Ticks, Jasmine L. Miller, Roger A. Lebrun, Howard S. Ginsberg

Senior Honors Projects

The nymphal stage of the blacklegged tick, Ixodes scapularis, is the major vector of Lyme disease, the most common vector-borne disease in North America. Tick abundance has generally been estimated using either flag/drag samples or samples from hosts. However, the biases of these sampling methods have not been adequately studied. We compared samples using both methods from sites in Massachusetts and Wisconsin. Tick abundance was compared with variables related to weather (temperature, relative humidity, and tick adverse moisture events), vegetation (canopy cover, tree density, shrub density, ground vegetation, and leaf litter cover), and host abundance (mice, small mammals, medium …