Entomology Commons^™

Assessment Of Variation In Susceptibility Of The Fall Armyworm, Spodoptera Frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), To Bacillus Thuringiensis Toxins, Karen Ferreira Da Silva

Department of Entomology: Dissertations, Theses, and Student Research

The fall armyworm, Spodoptera frugiperda, is a polyphagous insect pest affecting multiple crops. Fall armyworm is managed with insecticides and corn hybrids expressing insecticidal proteins derived from Bacillus thuringiensis. The early detection of insect resistance is important for making appropriate management decisions informs IPM and IRM recommendations.

The objective of the first study was to establish baseline susceptibility of fall armyworm populations to the Cry1F Bt insecticidal protein, emphasizing collections from locations where fall armyworm overwinters in the U.S. Fall armyworm neonates were exposed to artificial diet treated with increasing Cry1F concentrations, and mortality and growth inhibition were …

Monographic Revision Of The Genus Hoplopyga Thomson, 1880 (Coleoptera: Scarabaeidae: Cetoniinae: Gymnetini), Jennifer M. Shaughney

Department of Entomology: Dissertations, Theses, and Student Research

A comprehensive revision of the genus Hoplopyga Thomson, 1880 is presented. Eighteen species are redescribed and two new species are described. A key for identification, distribution maps, and images of the 20 species are provided. Hoplopyga ruteri Antoine is removed from the genus and placed in junior synonymy with Macrocranius similis Schürhoff. Lectotypes are designated for Hoplopyga brasiliensis (Gory and Percheron), Hoplopyga corumbana Schürhoff, and Hoplopyga multipunctata (Gory and Percheron). The following new synonyms are established: Hoplopyga lucidiventris (Thomson) is Hoplopyga foeda (Schaum); Hoplopyga spurca (Janson) is Hoplopyga liturata (Olivier); Hoplopyga monacha (Gory and Percheron) and Hoplopyga rubida …

Soil Preferences Of Nicrophorus Beetles And The Effects Of Compaction On Burying Behavior, Kelly A. Willemssens

School of Natural Resources: Dissertations, Theses, and Student Research

The American burying beetle, Nicrophorus americanus Olivier was declared federally endangered in 1989 and many efforts to prevent this species from going extinct are ongoing. The Nicrophorus beetles bury small carcasses for reproductive purposes. They also reside in the soil during times of daily and seasonal inactivity. To better understand why N. americanus is in decline, the importance of soil texture, moisture, vegetation, gravel, the burial depth, and the effect of compaction on their burying behavior was examined.

All tested species preferred moist soils with N. orbicollis having a significant preference for wet (pN. marginatus had a significant preference for …

Genetic Variability And Geographic Diversity Of The Common Bed Bug (Hemiptera: Cimicidae) Populations From The Midwest Using Microsatellite Markers, Ralph B. Narain, Sreedevi Madhusoodhanan Lalithambika, Shripat T. Kamble

Department of Entomology: Faculty Publications

With the recent global resurgence of the bed bugs (Cimex lectularius L.), there is a need to better understand its biology, ecology, and ability to establish populations. Bed bugs are domestic pests that feed mainly on mammalian blood. Although bed bugs have not been implicated as vectors of pathogens, their biting activity inflicts severe insomnia and allergic reactions. Moreover, they have recently developed resistance to various insecticides, which requires further molecular research to determine genetic variation and appropriate interventions. Population dynamics, including genetic differentiation and genetic distance of 10 populations from the Midwest were analyzed in this study. …

Evaluation Of Foraging Patterns Of Linepithema Humile (Mayr), The Argentine Ant, To Improve Bait Placement In Natural Park Habitats, Jinbo Song

All Dissertations

Linepithema humile (Mayr), the Argentine ant, is an invasive ant species and a significant pest in natural and managed habitats of the southeastern United States. In the natural sites of Lake Greenwood State Park (LGSP) in the Piedmont region of South Carolina, L. humile has invaded. Although park personnel treat problem areas with liquid insecticides, control of L. humile through the park areas is minimal. The primary objectives of this study were to determine the optimal foraging distance of L. humile, evaluate over-wintering nest temperatures and foraging activity of L. humile, and evaluate liquid bait placement to control L. humile. …

Crop Residue Disappearance And Macrofauna Activity In Sub-Humid Western Kenya, J. Kihara, C. Martius, A. Bationo

All Peer-Reviewed Publications

Rapid disappearance of crop residue used as soil surface cover in conservation tillage systems reduces the envisaged soil cover benefits. This study was conducted in a conservation tillage experiment, established in 2003 in Nyabeda, western Kenya, to (1) characterize crop residue disappearance during crop growth, and (2) assess termite activity and characteristics of soil (carbon concentration and aggregate stability) in termite-molded sheetings and mound soil. Loss of surface-placed residue in the presence of macrofauna (defined as >1 mm) was up to 83 % in 3.5 months, compared to 33 % in the absence of macrofauna. Overall, residue loss was up …

Monitoring Abundance Of Ips Bark Beetles And Determining Related Tree Mortality In Arkansas And Texas, Chandler Stefan Barton

Graduate Theses and Dissertations

The abundance of the southern pine engraver beetles, Ips avulsus (Eichhoff), I. grandicollis (Eichhoff), and I. calligraphus (Germar), was monitored with pheromone-baited traps in 2012 and 2013 in Arkansas and eastern Texas. Additionally, vehicle-based and comprehensive ground-based surveys were used to estimate and confirm the amount of dead and fading trees in each trapping site. Pine engraver beetles have historically been recognized as secondary invaders of healthy trees, but observations of pine mortality in Texas and Arkansas appeared to correspond with high population densities of Ips spp. Forest sites exhibiting subjectively high or low incidence of Ips-associated mortality in Arkansas …

Phylogeny And Diversity Of Entamoeba In Cockroaches, With An Emphasis On Periplaneta Americana, Mustafa Husain Fakhri

Graduate Theses and Dissertations

While the parasitic Amoebozoan Entamoeba histolytica has been well-studied for its role in human pathogenesis, the biodiversity of invertebrate-inhabiting Entamoeba has scarcely been investigated. Using molecular methods, I searched for Entamoeba in the guts of cockroaches from four of the six Blattodean families. Entamoeba small-subunit rRNA genes were recovered from all eight species of cockroaches tested, five of which represent newly discovered hosts. Phylogenetic analysis of over 190 sequences revealed a novel and highly diverse clade of cockroach-inhabiting Entamoeba, separate from the clade predominated by vertebrate-inhabitants. These results double the known genetic diversity of Entamoeba and suggest that they may …

Carbon Dioxide Sensitivity In Two Disjunct Populations Of The Pitcher-Plant Mosquito, Wyeomyia Smithii, Daniel L. Allen Jr.

Honors College Theses

The pitcher-plant mosquito, Wyeomyia smithii, utilizes carbon dioxide receptors primarily on their maxillary palps to seek potential hosts for blood meals. Two disjunct populations of W. smithii were analyzed to test for differences in carbon dioxide sensitivity that would correlate to varying levels of autogeny, ranging from the autogenous Northern populations (from North Carolina through Canada) to the anautonenous Southern populations (Florida – Louisiana), with the Georgia population exhibiting a shift from autogeny to anautogeny over the past two decades. I compared Georgia (Tattnall Co.) and Florida populations using blood feeding assays and olfactometry assays. Willingness to blood feed …

A Comprehensive Selection Of Reference Genes For Rt-Qpcr Analysis In A Predatory Lady Beetle, Hippodamia Convergens (Coleoptera: Coccinellidae), Huipeng Pan, Xiaowei Yang, Blair D. Siegfried, Xuguo Zhou

Entomology Faculty Publications

Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a reliable, rapid, and reproducible technique for measuring and evaluating changes in gene expression. To facilitate gene expression studies and obtain more accurate RT-qPCR data, normalization relative to stable reference genes is required. In this study, expression profiles of seven candidate reference genes, including β-actin (Actin), elongation factor 1 α (EF1A), glyceralde hyde-3-phosphate dehydro-genase (GAPDH), cyclophilins A (CypA), vacuolar-type H+-ATPase (ATPase), 28S ribosomal RNA (28S), and 18S ribosomal RNA (18S) from Hippodamia convergens were investigated. H. …

Producing Quality Barley For The Malting Industry, Haley H. Oser Dph

Doctor of Plant Health Program: Dissertations and Student Research

The University of Nebraska – Lincoln Doctor of Plant Health program requires each student to fulfill a professional internship over the last summer of the program. For my internship, I worked as a Barley Scientist Intern for MillerCoors and Golden Malting in Golden, Colorado. During this internship I gained a fundamental understanding of malting barley production with a significant emphasis on grain quality. Barley produced for malting must fulfill strict industry quality standards before it is accepted by the head maltsters. These quality standards include: a high germination rate, low moisture content, protein content within an acceptable range and the …

Mapk Signaling Pathway Alters Expression Of Midgut Alp And Abcc Genes And Causes Resistance To Bacillus Thuringiensis Cry1ac Toxin In Diamondback Moth, Zhaojiang Guo, Shi Kang, Defeng Chen, Qingjun Wu, Shaoli Wang, Wen Xie, Xun Zhu, Simon W. Baxter, Xuguo Zhou, Juan Luis Jurat-Fuentes, Youjun Zhang

Entomology Faculty Publications

Insecticidal crystal toxins derived from the soil bacterium Bacillus thuringiensis (Bt) are widely used as biopesticide sprays or expressed in transgenic crops to control insect pests. However, large-scale use of Bt has led to field-evolved resistance in several lepidopteran pests. Resistance to Bt Cry1Ac toxin in the diamondback moth, Plutella xylostella (L.), was previously mapped to a multigenic resistance locus (BtR-1). Here, we assembled the 3.15 Mb BtR-1 locus and found high-level resistance to Cry1Ac and Bt biopesticide in four independent P. xylostella strains were all associated with differential expression of a midgut membrane-bound alkaline phosphatase (ALP …

Phylogenetic Molecular Species Delimitations Unravel Potential New Species In The Pest Genus Spodoptera Guenée, 1852 (Lepidoptera, Noctuidae), Pascaline Dumas, Jérôme Barbut, Bruno Le Ru, Jean François Silvain, Anne Laure Clamens, Emmanuelle D'Alençon, Gael J. Kergoat

All Peer-Reviewed Publications

Nowadays molecular species delimitation methods promote the identification of species boundaries within complex taxonomic groups by adopting innovative species concepts and theories (e.g. branching patterns, coalescence). As some of them can efficiently deal with large single-locus datasets, they could speed up the process of species discovery compared to more time consuming molecular methods, and benefit from the existence of large public datasets; these methods can also particularly favour scientific research and actions dealing with threatened or economically important taxa. In this study we aim to investigate and clarify the status of economically important moths species belonging to the genus Spodoptera …

Water Vapor Uptake Across The Cocoon Wall Of The Introduced Pine Sawfly Diprion Similis (Hartig) (Hymenoptera: Diprionidae, Elizabeth Henderson

Thinking Matters Symposium Archive

For many Holarctic insect species, overwintering in a tightly spun cocoon provides protection against predators and pathogens, physical damage, and extreme fluctuations in environmental conditions. Research with insect cocoons has shown that the physical properties of the cocoon wall play an important role in regulating the diffusion of water vapor and respiratory gases. In an attempt to better understand properties of water vapor diffusion across the cocoon wall, we measured relative amounts of water uptake or loss in overwintering cocoons of the Introduced Pine Sawfly (Diprion similis (Hartig)) in Maine. We also characterized the relative density of silk fibers arranged …

Nebline, April 2015

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: 4-H Clubs of Excellence Award Recognizes Successful Clubs

Food & Health

Family Living

Farm & Acreage

Horticulture

Pests & Wildlife

4-H & Youth

Extension Calendar

and other extension news and events

Special Pullout Section: Weed Awareness

Eco-Evolutionary Factors Drive Herbivore-Induced Plant Volatiles, Which Intercept Plant Defense, Elizabeth K. Rowen

Open Access Theses

Herbivore-induced plant volatiles (HIPVs) mediate a wide variety of interactions in ecosystems. However, many volatile compounds are found across plant taxa, and some are exploited to attract natural enemies for biological control. Subtle changes in HIPV blends can produce profound changes in the behavior of predators, parasitoids and herbivores, and may also alter the defensive processes of neighboring plants. I investigated different ecological and evolutionary factors to understand how plant volatile emissions are affected by feeding guild, herbivore diet breadth and domestication. I meta-analyzed 109 studies and found that specialists induce more total volatiles than generalists. Domesticated species have stronger …

Potential Of Ozone Technology For German Cockroach (Bblattella Germanica (L.)) Management, Yanlin Tian

Open Access Theses

German cockroaches (Blattella germanica (L.)) are important urban pests that adversely impact human health. Previous studies indicated that ozone technology (ozone gas) can be used as a management tool to control insect pests in grain storage facilities, stored foods and some finished products. The goal of my study was to broaden the applicability of ozone from traditional stored grain pests to B. germanica. The first part of my study determined the concentration-time (CT) relationship of ozone required to achieve 100% mortality in various life stages (adults, nymphs and eggs) ofB. germanica. Results showed that eggs were the most ozone-tolerant …

Characterization Of Hessian Fly From Israel, Alisha J Johnson

Open Access Dissertations

Mayetiola destructor Say, the Hessian fly, is a gall midge and a member of the Dipteran family Cecidomyiidae. It is a common pest of wheat found throughout all of the major wheat growing areas of the world and poses a serious economic threat to the United States (US), particularly in the Southeast winter wheat region. Damage to wheat is done solely by feeding first and second in-star larvae. Hessian fly (Hf) infestations result in a loss in grain yield by the stunting and/or killing of seedling wheat plants in the winter and by causing breakage at the nodes of the …

Investigation Of Dried Distillers Grains With Solubles (Ddgs) Susceptibility To Red Flour Beetle (Tribolium Castaneum (Herbst)) Infestation, Mahsa Fardisi

Open Access Dissertations

Protecting livestock feed from insect infestation is a common challenge that feed facility managers and farmers have to deal with. It becomes an issue of importance because insect infestation degrades feed quality and quantity. Dried Distillers Grains with Solubles (DDGS) is added to livestock feed because of its high protein content. As DDGS usage in animal feed increases, understanding the susceptibility of DDGS, when mixed with livestock feed, to insect infestation is necessary for safe feed storage. This research addressed four questions. The first looked at the susceptibility of DDGS, when stored as a raw ingredient, to Tribolium castaneum (Herbst) …

The Role Of Entomology In Environmental And Science Education: Comparing Outreach Methods For Their Impact On Student And Teacher Content Knowledge And Motivation, Faith J. Weeks

Open Access Dissertations

Outreach programming can be an important way for local students and teachers to be exposed to new fields while enhancing classroom learning. University-based outreach programs are offered throughout the country, including most entomology departments as few individuals learn about insects in school and these programs can be excellent sources of entomological education, as well as models to teach environmental and science education. Each department utilizes different instructional delivery methods for teaching about insects, which may impact the way in which students and teachers understand the insect concepts presented. To determine the impact of using entomology to enhance science and environmental …

Undercover Predators: Vegetation Mediates Foraging, Trophic Cascades, And Biological Control By Omnivorous Weed Seed Predators, Carmen K Blubaugh

Open Access Dissertations

Weed pressure is the most costly challenge that vegetable growers face, requiring more labor investment than other production inputs. Vertebrate and invertebrate seed predators destroy a large percentage of weed propagules on the soil surface, and their ecosystem services may ease labor requirements for farmers in herbicide-free systems. Cover provided by living vegetation is an important predictor of seed predator activity, and my dissertation takes a comprehensive approach to understanding the behavior, predator, and environment-mediated mechanisms by which cover impacts weed seed predation in crop environments. ^ First, I performed a meta-analysis of 27 studies to quantitatively evaluate what is …

Carrion-Associated Arthropods In Rural And Urban Environments, Serena Daye Gross

Open Access Dissertations

Preferences of arthropods are important in forensic entomology, as the species collected can help determine the postmortem interval or if remains have been moved. This study looked at arthropods that are attracted to pig carcasses in rural and urban habitats in Northwest Indiana. The species and number of carrion-associated arthropods that are found at a carcass vary with habitat type, month, and stage of decomposition. Lucilia sericata (Diptera: Calliphoridae) is a synanthropic species that was found in higher numbers in urban locations for both adults and larvae. Though L. sericata had higher numbers in urban habitats, caution must be exercised …

Monarch Butterfly, Sam Baker

Sustainability

No abstract provided.

Internship In The Entomological Collections In The Smithsonian Institution (Si), National Museum Of Natural History (Nmnh): A Species-Level Inventory Of The Superfamily Gelechioidea (Insecta: Lepidoptera)., Sherry V. Spriggs

Department of Entomology: Distance Master of Science Projects

The internship was completed in the Department of Entomology at the Smithsonian Institution’s National Museum of Natural History located in Washington, DC. The internship started November 2014 and ended April 2015 and consisted of working in the collection room one day per week, usually on Tuesdays. One of the goals of the Department of Entomology, National Museum of Natural History, Smithsonian Institution is to compile a comprehensive inventory all of the species in its collections. As a component of this over-arching goal, the primary project of the internship was to compile a species-level inventory of the superfamily Gelechioidea (Insecta: Lepidoptera).

Comparison Of The Genome Profiles Between Head And Body Lice, Jae Soon Kang, Yong-Ho Cho, Ju Hyeon Kim, Sang Hyeon Kim, Seungil Yoo, Seung-Jae Noh, Junhyung Park, Kyong-Sup Yoon, J. Marshall Clark, Barry R. Pittendrigh, Jongsik Chun, Si Hyeock Lee

Biological Sciences Faculty Research, Scholarship, and Creative Activity

The body louse (Pediculus humanus humanus) is known to have diverged from the head louse (P. humanus capitis) but genomic differences between these two subspecies still remain unexplored. To compare genomic profiles between head and body lice, whole genome sequences of head lice were determined by next generation sequencing methods based on both Illumina Genome analyzer and Roche GS FLX pyrosequencing and compared with the reference genome sequences of the body louse. Total consensuses generated by mapping to the body louse genome in conjunction with de novo assembly of head louse genome sequences revealed a head louse genome size of …

Non-Target Effects Of Clothianidin On Monarch Butterflies, Jacob R. Pecenka, Jonathan G. Lundgren

Natural Resource Management Faculty Publications

Monarch butterflies (Danaus plexippus) frequently consume milkweed in and near agroecosystems and consequently may be exposed to pesticides like neonicotinoids. We conducted a dose response study to determine lethal and sublethal doses of clothianidin using a 36-h exposure scenario. We then quantified clothianidin levels found in milkweed leaves adjacent to maize fields. Toxicity assays revealed LC₁₀, LC₅₀, and LC₉₀ values of 7.72, 15.63, and 30.70 ppb, respectively. Sublethal effects (larval size) were observed at 1 ppb. Contaminated milkweed plants had an average of 1.14 ±0.10 ppb clothianidin, with a maximum of 4 ppb in a …

Responses Of Parasitoids To Volatiles Induced By Chilo Partellus Oviposition On Teosinte, A Wild Ancestor Of Maize, Daniel M. Mutyambai, Toby J.A. Bruce, Charles A.O. Midega, Christine M. Woodcock, John C. Caulfield, Johnnie Van Den Berg, John A. Pickett, Zeyaur R. Khan

All Peer-Reviewed Publications

Maize, a genetically diverse crop, is the domesticated descendent of its wild ancestor, teosinte. Recently, we have shown that certain maize landraces possess a valuable indirect defense trait not present in commercial hybrids. Plants of these landraces release herbivore-induced plant volatiles (HIPVs) that attract both egg [Trichogramma bournieri Pintureau & Babault (Hymenoptera: Trichogrammatidae)] and larval [Cotesia sesamiae Cameron (Hymenoptera: Braconidae)] parasitoids in response to stemborer egg deposition. In this study, we tested whether this trait also exists in the germplasm of wild Zea species. Headspace samples were collected from plants exposed to egg deposition by Chilo partellus Swinhoe (Lepidoptera: Crambidae) …

Flea Beetles On Vegetables, Bonnie Bunn, Diane Alston, Marion Murray

All Current Publications

Flea beetles are common and problematic in Utah. They are present in late spring and early summer on many vegetable crops and ornamental plants. Adult flea beetles are small, shiny insects that have enlarged hind legs, allowing them to jump great distances when disturbed.

Pyrosequencing Reveals A Shift In Symbiotic Bacteria Populations Across Life Stages Of Bactrocera Dorsalis, Awawing A. Andongma, Lun Wan, Yong-Cheng Dong, Ping Li, Nicolas Desneux, Jennifer A. White, Chang-Ying Niu

Entomology Faculty Publications

Bactrocera dorsalis is one of the most economically important fruit flies around the world. In this study, 454 pyrosequencing was used to identify the bacteria associated with different developmental stages of B. dorsalis. At ≥ 97% nucleotide similarity, total reads could be assigned to 172 Operational Taxonomic Units belonging to six phyla. Proteobacteria dominated in immature stages while Firmicutes dominated in adult stages. The most abundant families were Enterococcaceae and Comamondaceae. The genus Comamonas was most abundant in pupae whereas completely absent in adults. Some identified species had low sequence similarity to reported species indicating the possibility of …

Stably Expressed Housekeeping Genes Across Developmental Stages In The Two-Spotted Spider Mite, Tetranychus Urticae, Chunxiao Yang, Huipeng Pan, Yong Liu, Xuguo Zhou

Entomology Faculty Publications

Quantitative real-time PCR (qRT-PCR) is a reliable and reproducible technique for measuring mRNA expression. To facilitate gene expression studies and obtain more accurate qRT-PCR analysis, normalization relative to stable housekeeping genes is mandatory. In this study, ten housekeeping genes, including beta-actin (Actin), elongation factor 1 α (EF1A), glyceralde hyde-3-phosphate dehydrogenase (GAPDH), ribosomal protein L13 (RPL13), ribosomal protein 49 (RP49), α-tubulin (Tubulin), vacuolar-type H+-ATPase (v-ATPase), succinate dehydrogenase subunit A (SDHA) , 28S ribosomal RNA (28S), and 18S ribosomal RNA (18S) from …