Entomology Commons^™

Suitability Of Blue Ash (Fraxinus Quadrangulata) And Green Ash (F. Pennsylvanica) To Emerald Ash Borer (Agrilus Planipennis) And Its Larval Parasitoid Tetrastichus Planipennisi., Donnie "L. " Peterson

Open Access Theses

Emerald ash borer (EAB) (Agrilus planipennis ) is a primary pest that has killed tens of millions of North American ash (Fraxinus spp.) trees. The larval parasitoid Tetrastichus planipennisi was introduced from China as part of a classical biological control program for long-term EAB management. The high mortality rates of ash trees greatly reduce the number of EAB hosts and may make it difficult for parasitoids to persist. However, blue ash ( F. quadrangulata ) is relatively resistant and appears to be able to survive EAB infestation. If natural enemies can attack EAB in infested blue ash they …

Nebline, October 2014

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Smart Snacking

Food & Health

Family Living

Farm & Acreage

Home & Family Living

Horticulture

Pests & Wildlife

4-H & Youth

Extension Calendar

and other extension news and events

The Role Of Chemical Attractants In The Use Of Tick Traps For Life Stages Of Tick Species Ixodes Scapularis, Dermacentor Variabilis And Amblyomma Maculatum, Pamela C. Kelman

Biological Sciences Theses & Dissertations

Amblyomma maculatum, Ixodes scapularis and Dermacentor variabilis are three of the hard-bodied ticks found in the United States. This study explored three aspects of the appetence process of these tick species: attraction, desiccation survival, and locomotive activity. The first portion of this work was to improve one of the standard methods used for collecting ticks from the environment, the tick trap. To determine the attractants needed to capture all life stages of the three chosen species of ticks in the field using tick traps, a series of experiments were conducted using laboratory-reared larval, nymphal and adult A. maculatum, I. scapularis …

Dangerous Mating Systems: Signal Complexity, Signal Content And Neural Capacity In Spiders, Marie E. Herberstein, Anne E. Wignall, Eileen Hebets, Jutta M. Schneider

Eileen Hebets Publications

Spiders are highly efficient predators in possession of exquisite sensory capacities for ambushing prey, combined with machinery for launching rapid and determined attacks. As a consequence, any sexually motivated approach carries a risk of ending up as prey rather than as a mate. Sexual selection has shaped courtship to effectively communicate the presence, identity, motivation and/or quality of potential mates, which help ameliorate these risks. Spiders communicate this information via several sensory channels, including mechanical (e.g. vibrational), visual and/or chemical, with examples of multimodal signaling beginning to emerge in the literature. The diverse environments that spiders inhabit have further shaped …

Multimodal Sensory Reliance In The Nocturnal Homing Of The Amblypygid Phrynus Pseudoparvulus (Class Arachnida, Order Amblypygi)?, Eileen A. Hebets, Alfonso Aceves-Aparicio, Samuel Aguilar-Argüello, Verner P. Bingman, Ignacio Escalante, Eben J. Gering, David R. Nelson, Jennifer Rivera, José Ángel Sánchez-Ruiz, Laura Segura-Hernández, Virginia Settepani, Daniel D. Wiegmann, Jay A. Stafstrom

Eileen Hebets Publications

Like many other nocturnal arthropods, the amblypygid Phrynus pseudoparvulus is capable of homing. The environment through which these predators navigate is a dense and heterogeneous tropical forest understory and the mechanism(s) underlying their putatively complex navigational abilities are presently unknown. This study explores the sensory inputs that might facilitate nocturnal navigation in the amblypygid P. pseudoparvulus. Specifically, we use sensory system manipulations in conjunction with field displacements to examine the potential involvement of multimodal—olfactory and visual—stimuli in P. pseudoparvulus’ homing behavior. In a first experiment, we deprived individuals of their olfactory capacity and displaced them to the opposite side of …

Stability, Bifurcation And Chaos Analysis Of Vector-Borne Disease Model With Application To Rift Valley Fever, Sansao A. Pedro, Shirley Abelman, Frank T. Ndjomatchoua, Rosemary Sang, Henri E.Z. Tonnang

All Peer-Reviewed Publications

This paper investigates a RVF epidemic model by qualitative analysis and numerical simulations. Qualitative analysis have been used to explore the stability dynamics of the equilibrium points while visualization techniques such as bifurcation diagrams, Poincaré maps, maxima return maps and largest Lyapunov exponents are numerically computed to confirm further complexity of these dynamics induced by the seasonal forcing on the mosquitoes oviposition rates. The obtained results show that ordinary differential equation models with external forcing can have rich dynamic behaviour, ranging from bifurcation to strange attractors which may explain the observed fluctuations found in RVF empiric outbreak data, as well …

Microclimate Modification And Insect Pest Exclusion Using Agronet Improve Pod Yield And Quality Of French Bean, Elisha Otieno Gogo, Mwanarusi Saidi, Jacob Mugwa Ochieng, Thibaud Martin, Vance Baird, Mathieu Ngouajio

All Peer-Reviewed Publications

French bean [Phaseolus vulgaris (L.)] is among the leading export vegetable in Africa, mostly produced by small-scale farmers. Unfavorable environmental conditions and heavy infestations by insect pests are among the major constraints limiting production of the crop. Most French bean producers grow their crop in open fields outdoors subject to harsh environmental conditions and repeatedly spray insecticides in a bid to realize high yield. This has led to rejection of some of the produce at the export market as a result of stringent limits on maximum residue levels. Two trials were conducted at the Horticulture Research and Teaching Field, Egerton …

Structural Characterization Of Nanofiber Silk Produced By Embiopterans (Webspinners), J. Bennett Addison, Thomas M. Osborn Popp, Warner S. Weber, Janice Edgerly-Rooks, Gregory P. Holland, Jeffery L. Yarger

Biology

Embiopterans produce silken galleries and sheets using exceptionally fine silk fibers in which they live and breed. In this study, we use electron microscopy (EM), Fourier-transform infrared (FT-IR) spectroscopy, wide angle X-ray diffraction (WAXD) and solid-state nuclear magnetic resonance (ssNMR) techniques to elucidate the molecular level protein structure of webspinner (embiid) silks. Silks from two species Antipaluria urichi and Aposthonia ceylonica are studied in this work. Electron microscopy images show that the fibers are about 90–100 nm in diameter, making webspinner silks among the finest of all known animal silks. Structural studies reveal that the silk protein core is dominated …

De Novo Transcriptome Of The Hemimetabolous German Cockroach (Blattella Germanica), Xiaojie Zhou, Kun Qian, Ying Tong, Junwei Jerry Zhu, Xinghui Qiu, Xiaopeng Zeng

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Background: The German cockroach, Blattella germanica, is an important insect pest that transmits various pathogens mechanically and causes severe allergic diseases. This insect has long served as a model system for studies of insect biology, physiology and ecology. However, the lack of genome or transcriptome information heavily hinder our further understanding about the German cockroach in every aspect at a molecular level and on a genome-wide scale. To explore the transcriptome and identify unique sequences of interest, we subjected the B. germanica transcriptome to massively parallel pyrosequencing and generated the first reference transcriptome for B. germanica.

Methodology/Principal Findings: A …

Colonization Of Onions By Endophytic Fungi And Their Impacts On The Biology Of Thrips Tabaci, Alexander M. Muvea, Rainer Meyhöfer, Sevgan Subramanian, Hans Michael Poehling, Sunday Ekesi, Nguya K. Maniania

All Peer-Reviewed Publications

Endophytic fungi, which live within host plant tissues without causing any visible symptom of infection, are important mutualists that mediate plant-herbivore interactions. Thrips tabaci (Lindeman) is one of the key pests of onion, Allium cepa L., an economically important agricultural crop cultivated worldwide. However, information on endophyte colonization of onions, and their impacts on the biology of thrips feeding on them, is lacking. We tested the colonization of onion plants by selected fungal endophyte isolates using two inoculation methods. The effects of inoculated endophytes on T. tabaci infesting onion were also examined. Seven fungal endophytes used in our study were …

Predicting The Impact Of Climate Change On Regional And Seasonal Abundance Of The Mealybug Phenacoccus Solenopsis Tinsley (Hemiptera: Pseudococcidae) Using Temperature-Driven Phenology Model Linked To Gis, Babasaheb B. Fand, Henri E.Z. Tonnang, Mahesh Kumar, Santanu K. Bal, Naveen P. Singh, D. V.K.N. Rao, Ankush L. Kamble, Dhananjay D. Nangare, Paramjit S. Minhas

All Peer-Reviewed Publications

The mealybug Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) is a highly invasive and polyphagous pest of global incidence. The fundamental hypothesis of the present study was that the temperature variations due to global climate change may affect seriously the future distribution and abundance of P. solenopsis, which might further aggravate the crop yield losses. We employed a temperature-based phenology model of P. solenopsis in a geographic information system for mapping population growth potentials of P. solenopsis. The three risk indices viz., establishment risk index, generation index and activity index were computed using interpolated temperature data from worldclim database for current (2000) …

Field Evaluation Of Natural Human Odours And The Biogent-Synthetic Lure In Trapping Aedes Aegypti, Vector Of Dengue And Chikungunya Viruses In Kenya, Eunice A. Owino, Rosemary Sang, Catherine L. Sole, Christian Pirk, Charles Mbogo, Baldwyn Torto

All Peer-Reviewed Publications

Background: Methods currently used in sampling adult Aedes aegypti, the main vector of dengue and chikungunya viruses are limited for effective surveillance of the vector and accurate determination of the extent of virus transmission during outbreaks and inter - epidemic periods. Here, we document the use of natural human skin odours in baited traps to improve sampling of adult Ae. aegypti in two different endemic areas of chikungunya and dengue in Kenya - Kilifi and Busia Counties. The chemistry of the volatiles released from human odours and the Biogent (BG)-commercial lure were also compared.

Vector Competence Of Aedes Aegypti Populations From Kilifi And Nairobi For Dengue 2 Virus And The Influence Of Temperature, Edith Chepkorir, Joel Lutomiah, James Mutisya, Francis Mulwa, Konongoi Limbaso, Benedict Orindi, Zipporah Ng'ang'a, Rosemary Sang

All Peer-Reviewed Publications

Background: Susceptibility of Ae. aegypti mosquito to dengue virus (DENV) varies geographically and can be influenced by climatic factors such as temperature, which affect the incidence, seasonality and distribution of vector-borne diseases. The first outbreak of dengue fever (DF) in Kenya occured in 1982 in the coastal towns of Malindi and Kilifi. Unlike Nairobi where no active dengue transmission has been reported, DF is currently re-emerging at the Coast causing major outbreaks. This study investigated the vector competence of Ae. aegypti populations from two urban areas, Kilifi (Coast of Kenya) and Nairobi (Central Kenya), for DEN-2 virus and the influence …

Organic Treatments For Control Of Pepper Weevil (Coleoptera: Curculionidae), Karla M. Addesso, Philip A. Stansly, Barry C. Kostyk, Heather J. Mcauslane

Agricultural and Environmental Sciences Faculty Research

The pepper weevil, Anthonomus eugenii Cano (Coleoptera: Curculionidae) is a major pest of pepper (Capsicum spp.; Solanales: Solanaceae) in the southern United States, Mexico, Central America and the Caribbean. Feeding and oviposition cause flower and fruit abscission and internal fruit damage resulting in serious yield losses. Females lay eggs in flower buds and small fruits, shielding larvae from contact pesticides, leaving only the adult stage vulnerable. The purpose of this study was to investigate low-risk and organic products for use against the pepper weevil to provide both organic and conventional growers with more control options. A neem product (Ecozin® …

Nebline, September 2014

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: 4-H Youth Showcase Their Achievements at Super Fair

Food & Health

Family Living

Farm & Acreage

Home & Family Living

Horticulture

Pests & Wildlife

4-H & Youth

Extension Calendar

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Factors Limiting The Spread Of The Protective Symbiont Hamiltonella Defensa In Aphis Craccivora Aphids, Hannah R. Dykstra, Stephanie R. Weldon, Adam J. Martinez, Jennifer A. White, Keith R. Hopper, George E. Heimpel, Mark K. Asplen, Kerry M. Oliver

Entomology Faculty Publications

Many insects are associated with heritable symbionts that mediate ecological interactions, including host protection against natural enemies. The cowpea aphid, Aphis craccivora, is a polyphagous pest that harbors Hamiltonella defensa, which defends against parasitic wasps. Despite this protective benefit, this symbiont occurs only at intermediate frequencies in field populations. To identify factors constraining H. defensa invasion in Ap. craccivora, we estimated symbiont transmission rates, performed fitness assays, and measured infection dynamics in population cages to evaluate effects of infection. Similar to results with the pea aphid, Acyrthosiphon pisum, we found no consistent costs to infection using …

Influence Of Resource Levels, Organic Compounds And Laboratory Colonization On Interspecific Competition Between The Asian Tiger Mosquito Aedes Albopictus (Stegomyia Albopicta) And The Southern House Mosquito Culex Quinquefasciatus, D.W. Allgood, Donald A. Yee

Faculty Publications

The mosquitoes Aedes albopictus (Stegomyia albopicta) (Skuse) and Culex quinquefasciatus (Say) (Diptera: Culicidae) are common inhabitants of tyres and other artificial containers, which constitute important peridomestic mosquito breeding habitats. We tested the hypotheses that interspecific resource competition between the larvae of these species is asymmetrical, that the concentration of chemicals associated with decomposing detritus affects the competitive outcomes of these species, and that wild and colonized strains of Cx. quinquefasciatus are affected differently by competition with Ae. albopictus. We conducted two laboratory competition experiments wherein we measured survivorship and estimated population growth (λ′) in both species under …

Life History Of Paracoccus Marginatus (Hemiptera: Pseudococcidae) On Four Host Plant Species Under Laboratory Conditions, Kaushalya G. Amarasekare, Catharine M. Mannion, Lance S. Osborne, Nancy D. Epsky

Agricultural and Environmental Sciences Faculty Research

Life history of the mealybug, Paracoccus marginatus Williams and Granara de Willink, on three ornamental plants [Hibiscus rosa-sinensis L., Acalypha wilkesiana (Muell.-Arg.), and Plumeria rubra L.] and one weed species (Parthenium hysterophorus L.) was studied under laboratory conditions. Mealybugs were able to develop, survive, and reproduce on all four hosts; however, there were differences in the life history parameters. Adult females that developed on acalypha and parthenium emerged ≈1 d earlier than those that developed on hibiscus and plumeria. Adult males had a longer developmental time on plumeria than on the other hosts. Survival of first- and second-instar …

Sma-Causing Missense Mutations In Survival Motor Neuron (Smn) Display A Wide Range Of Phenotypes When Modeled In Drosophila, Kavita Praveen, Ying Wen, Kelsey M. Gray, John J. Noto, Akash R. Patlolla, Gregory D. Van Duyne, A. Gregory Matera

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Mutations in the human survival motor neuron 1 (SMN) gene are the primary cause of spinal muscular atrophy (SMA), a devastating neuromuscular disorder. SMN protein has a well-characterized role in the biogenesis of small nuclear ribonucleoproteins (snRNPs), core components of the spliceosome. Additional tissue-specific and global functions have been ascribed to SMN; however, their relevance to SMA pathology is poorly understood and controversial. Using Drosophila as a model system, we created an allelic series of twelve Smn missense mutations, originally identified in human SMA patients. We show that animals expressing these SMA-causing mutations display a broad range of …

Molecular Xenomonitoring Using Mosquitoes To Map Lymphatic Filariasis After Mass Drug Administration In American Samoa, Mark A. Schmaedick, Amanda L. Koppel, Nils Pilotte, Melissa Torres, Steven A. Williams, Stephen L. Dobson, Patrick J. Lammie, Kimberly Y. Won

Entomology Faculty Publications

BACKGROUND: Mass drug administration (MDA) programs have dramatically reduced lymphatic filariasis (LF) incidence in many areas around the globe, including American Samoa. As infection rates decline and MDA programs end, efficient and sensitive methods for detecting infections are needed to monitor for recrudescence. Molecular methods, collectively termed 'molecular xenomonitoring,' can identify parasite DNA or RNA in human blood-feeding mosquitoes. We tested mosquitoes trapped throughout the inhabited islands of American Samoa to identify areas of possible continuing LF transmission after completion of MDA.

METHODOLOGY/PRINCIPLE FINDINGS: Mosquitoes were collected using BG Sentinel traps from most of the villages on American Samoa's largest …

Nebline, August 2014

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Cherry Creek Habitat Provides Opportunities for Pollinator Education

Food & Health

Family Living

Farm & Acreage

Home & Family Living

Horticulture

Pests & Wildlife

4-H & Youth

Extension Calendar

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Special pullout section: Lancaster County Super Fair 2014 Schedule & Map

Hexapod Herald - Vol. 26, No. 4, August 2014

Hexapod Herald and Other Entomology Department Newsletters

Contents: Welcome - Congratulations - Faculty News - Publications - Grants - Travel - Meet an Off-Campus Student - Student News

Repair Of The Proboscis Of Brush-Footed Butterflies (Lepidoptera: Nymphalidae), Suellen Pometto

All Theses

A key feature of the order Lepidoptera is the coilable proboscis, present in over 99% of lepidopteran species. The proboscis is used to obtain liquid nutrition, usually floral nectar. The proboscis is assembled from two elongate galeae immediately after emergence of the adult from the pupa. What happens if the galeae become separated? I studied the process of repair of the proboscis, behaviorally and functionally, at the organismal level. My research questions were as follows: 1) is the proboscis capable of repair, 2) is saliva necessary to proboscis repair, and 3) is the repaired proboscis able to acquire fluids? Test …

An Evaluation Of Watermelon (Citrullus Spp.) Germplasm For Additional Sources Of Resistance To The Twospotted Spider Mite (Tetranychus Urticae Koch)., Hector Cantu Jr.

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Fourteen U.S plant introduction (PI) accessions of C. lanatus var. lanatus (4), C. lanatus var. citroides (5) C. colocynthis (5) and a known susceptible commercial cultivar ‘Sugar Baby’ were evaluated for resistance to the twospotted spider mite, Tetranychus urticae Koch, in a limited free-choice and free-choice bioassay under laboratory conditions. The limited free choice bioassay, involved nine Petri dish cages that held five randomly assigned leaves individually inoculated with two adult females and one adult male. Eggs, larva, and adults were counted over a nine day period. The free choice bioassay involved the even distribution of three mite infested …

Host Specificity And Ectoparasite Load Of Bat Flies In Utila, Honduras, Courtney Miller

Senior Honors Theses

Bat flies (Streblidae) are obligate blood-feeding ectoparasites of bats that display varying degrees of host specificity. A total of 265 streblid bat flies were collected from 122 bats belonging to the families Phyllostomidae and Natalidae from Utila, the smallest bay island of Honduras. Out of four host-parasite associations, three were considered primary. Out of the three bat species analyzed, one had significantly lower parasite prevalence and another had significantly higher parasite load and intensity. Both male and female bats were equally likely to be infested and variables of parasite density did not differ amongst host sex for any species. However, …

Rna Interference As A Tool For The Functional Analysis Of Genes In The Colorado Potato Beetle, Leptinotarsa Decemlineata (Say), Ashley Danielle Yates

Department of Entomology: Dissertations, Theses, and Student Research

RNA interference (RNAi) is a naturally occurring phenomenon in eukaryotes in which a double-stranded RNA (dsRNA) suppresses the expression of a target gene. RNAi has markedly changed the way in which functional genetics studies are performed, especially in non-model organisms. In insects, the efficacy of RNAi is influenced by several factors, including the species and the methods of dsRNAs delivery.

The Colorado potato beetle (CPB), Leptinotarsa decemlineata (Say), is a pest of the plant family Solanaceae. RNAi in the CPB is of interest for potential use in insect management and as a tool to study the interaction with host plants. …

Characterization Of Greenbug Feeding Behavior And Aphid (Hemiptera: Aphididae) Host Preference In Relation To Resistant And Susceptible Tetraploid Switchgrass Populations, Kyle G. Koch, Nathan Palmer, Mitch Stamm, Jeff D. Bradshaw, Erin Blankenship, Lisa M. Baird, Gautam Sarath, Tiffany M. Heng-Moss

Department of Entomology: Faculty Publications

Two choice studies were performed to evaluate greenbug, Schizaphis graminum (Rondani), and yellow sugarcane aphid, Sipha flava (Forbes), preference for two tetraploid switchgrass populations, Summer and Kanlow, and one experimental hybrid, K×S, derived by crossing Kanlow (male)×Summer (female) plants. Additionally, an assessment of S. graminum feeding behavior was performed on the same switchgrass populations, by using the electrical penetration graph (EPG) technique. Choice studies for S. flava indicated a lack of antixenosis, with no preference by aphids among any of the switchgrass populations at any time point. However, choice studies with S. graminum indicated a preference for the K×S plants …

Push-Pull: A Novel Ipm Strategy For The Green Revolution In Africa, Zeyaur R. Khan, Charles A.O. Midega, Jimmy O. Pittchar, John A. Pickett

All Peer-Reviewed Publications

Africa faces serious challenges in feeding its population, mainly due to poor yields of cereals that serve as both staple and cash crops occasioned by insect pests, weeds, and poor soil fertility, and more recently effects of climate change. A novel IPM approach dubbed push-pull has been developed and implemented in eastern Africa, based on locally available companion plants, that effectively addresses these constraints resulting in substantial grain yield increases. The technology involves intercropping cereal crops with stemborer moth repellent crops (push), the forage legume desmodium or molasses grass, and planting the attractive Napier grass (pull) as a border crop. …

Similar Differentiation Patterns Between Pbp Expression Levels And Pheromone Component Ratios In Two Populations Of Sesamia Nonagrioides, Nicolas Glaser, Brigitte Frérot, Ene Leppik, Christelle Monsempes, Claire Capdevielle-Dulac, Bruno Le Ru, Thomas Lecocq, Myriam Harry, Emmanuelle Jacquin-Joly, Paul Andre Calatayud

All Peer-Reviewed Publications

Pheromone-binding proteins (PBPs) are thought to contribute to the specificity of the pheromone detection system through an initial selective binding with pheromone molecules. Here, we report different expression levels of PBP transcripts in the antennae of two populations of the stemborer Sesamia nonagrioides (Lepidoptera: Noctuidae), one collected in Europe and one in sub-Saharan Africa. The three PBP transcripts previously identified in this species were found to be expressed in both male and female antennae. Whereas PBP3 did not show any differential expression, PBP1 and PBP2 appeared to be expressed differently according to the population origin and sex. Simultaneously, we measured …

Potential Interactions Of Early Season Herbicides And Insecticides In Cotton: Thrips Control And Plant Health, Derek Lane Clarkson

Graduate Theses and Dissertations

Cotton growth early in the season is affected by damaging levels of thrips in many production fields in Arkansas. During this time, insecticides used to control thrips and herbicides used to control weeds are often present at the same time on the cotton plant. This research explores how various combinations of herbicides and insecticides influence thrips numbers and cotton plant growth parameters early in the growing season. Pathways evaluated included interactions between preemergence herbicides and insecticide seed treatments and interactions between common tank-mixed foliar herbicides and selected foliar insecticides. No interactions in thrips control or plant growth were observed when …