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Full-Text Articles in Entomology

An Update Of The Captive Management And Reintroduction Of The Salt Creek Tiger Beetle, Cicindela Nevadica Lincolniana (Coleoptera:Carabidae) At Omaha’S Henry Doorly Zoo & Aquarium, Sarah Jenkins, Traci Clevenger Jan 2015

An Update Of The Captive Management And Reintroduction Of The Salt Creek Tiger Beetle, Cicindela Nevadica Lincolniana (Coleoptera:Carabidae) At Omaha’S Henry Doorly Zoo & Aquarium, Sarah Jenkins, Traci Clevenger

Entomology Papers from Other Sources

Tiger beetles are a high speed predator, both as larvae and adults. There are over 2,600 species found worldwide. They are so fast that their eyes can’t gather enough light to process visual information while running and the beetles have to pause during pursuit to regain sight of their target. The fastest known tiger beetle can run up to 8 kilometers per hour (5 mph) which is comparable to a human running 772.5 kph (480 mph) when adjusted for body length (Yong, 2014). The adults are sexually dimorphic, with males possessing short white hairs on the inside of the tarsi …


Note On Ehrlichia Chaffeensis, Ehrlichia Ewingii, And “Borrelia Lonestari” Infection In Lone Star Ticks (Acari: Ixodidae), Nebraska, Usa, Amanda C. Maegli, J. Dustin Loy, Roberto Cortinas Jan 2015

Note On Ehrlichia Chaffeensis, Ehrlichia Ewingii, And “Borrelia Lonestari” Infection In Lone Star Ticks (Acari: Ixodidae), Nebraska, Usa, Amanda C. Maegli, J. Dustin Loy, Roberto Cortinas

Department of Entomology: Faculty Publications

The lone star tick, Amblyomma americanum (L.) (Acari: Ixodidae), is established in southeastern Nebraska yet the prevalence of tick-associated microorganisms is not known. An initial PCR-based analysis for Ehrlichia chaffeensis, Ehrlichia ewingii, and Borrelia infection in host-seeking adult ticks collected in southeast Nebraska was conducted. A total of 251 adult ticks collected in six sites in southeast Nebraska were tested. E. chaffeensis, E. ewingii, and Borrelia spp. were present, and the prevalence of each was approximately 1.6%. This study demonstrates that Ehrlichia spp. are present in Nebraska lone star tick populations.


Nebline, January 2015 Jan 2015

Nebline, January 2015

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Extension 2014 Highlights

Food & Health

Family Living

Farm & Acreage

Horticulture

Pests & Wildlife

4-H & Youth

Extension Calendar

and other extension news and events


Effect Of Seed Blends And Soil-Insecticide On Western And Northern Corn Rootworm Emergence From Mcry3a1+ Ecry3.1ab Bt Maize, Daniel L. Frank, Ryan Kurtz, N A. Tinsley, Aaron J. Gassmann, Lance J. Meinke, Daniel Moellenbeck, Michael E. Gray, Larry W. Bledsoe, Christan H. Krupke, Ronald E. Estes, Patrick Weber, Bruce E. Hibbard Jan 2015

Effect Of Seed Blends And Soil-Insecticide On Western And Northern Corn Rootworm Emergence From Mcry3a1+ Ecry3.1ab Bt Maize, Daniel L. Frank, Ryan Kurtz, N A. Tinsley, Aaron J. Gassmann, Lance J. Meinke, Daniel Moellenbeck, Michael E. Gray, Larry W. Bledsoe, Christan H. Krupke, Ronald E. Estes, Patrick Weber, Bruce E. Hibbard

Department of Entomology: Faculty Publications

Seed blends containing various ratios of transgenic Bt maize (Zea mays L.) expressing the mCry3A+eCry3.1Ab proteins and non-Bt maize (near-isoline maize) were deployed alone and in combination with a soil applied pyrethroid insecticide (Force CS) to evaluate the emergence of the western corn rootworm, Diabrotica virgifera virgifera LeConte, in a total of nine field environments across the Midwestern United States in 2010 and 2011. Northern corn rootworm, Diabrotica barberi Smith & Lawrence emergence was also evaluated in four of these environments. Both western and northern corn rootworm beetle emergence from all Bt treatments was significantly reduced when compared with beetle …


Parental Rna Interference Of Genes Involved In Embryonic Development Of The Western Corn Rootworm, Diabrotica Virgifera Virgifera Leconte, Chitvan Khajuria, Ana Maria Vélez, Murugesan Rangasamy, Haichuan Wang, Elane Fishilevich, Meghan L.F. Frey, Newton Portilho Carneiro, Premchand Gandra, Kenneth E. Narva, B. D. Siegfried Jan 2015

Parental Rna Interference Of Genes Involved In Embryonic Development Of The Western Corn Rootworm, Diabrotica Virgifera Virgifera Leconte, Chitvan Khajuria, Ana Maria Vélez, Murugesan Rangasamy, Haichuan Wang, Elane Fishilevich, Meghan L.F. Frey, Newton Portilho Carneiro, Premchand Gandra, Kenneth E. Narva, B. D. Siegfried

Department of Entomology: Faculty Publications

RNA interference (RNAi) is being developed as a potential tool for insect pest management and one of the most likely target pest species for transgenic plants that express double stranded RNA (dsRNA) is the western corn rootworm. Thus far, most genes proposed as targets for RNAi in rootworm cause lethality in the larval stage. In this study, we describe RNAi-mediated knockdown of two developmental genes, hunchback (hb) and brahma (brm), in the western corn rootworm delivered via dsRNA fed to adult females. dsRNA feeding caused a significant decrease in hb and brm transcripts in the adult …


Selection Of Reference Genes For Rt-Qpcr Analysis In The Monarch Butterfly, Danaus Plexippus (L.), A Migrating Bio-Indicator, Huipeng Pan, Xiaowei Yang, Keith Bidne, Richard L. Hellmich, Blair D. Siegfried, Xuguo Zhou Jan 2015

Selection Of Reference Genes For Rt-Qpcr Analysis In The Monarch Butterfly, Danaus Plexippus (L.), A Migrating Bio-Indicator, Huipeng Pan, Xiaowei Yang, Keith Bidne, Richard L. Hellmich, Blair D. Siegfried, Xuguo Zhou

Department of Entomology: Faculty Publications

Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a powerful technique to quantify gene expression. To facilitate gene expression study and obtain accurate results, normalization relative to stably expressed reference genes is crucial. The monarch butterfly, Danaus plexippus (L.), is one of the most recognized insect species for its spectacular annual migration across North America. Besides its great voyages, D. plexippus has drawn attention to its role as a bio-indicator, ranging from genetically modified organisms (GMOs) to natural ecosystems. In this study, nine reference genes from D. plexippus genome were selected as the candidate reference genes. The expression profiles of these …


Early Detection And Mitigation Of Resistance To Bt Maize By Western Corn Rootworm (Coleoptera: Chrysomelidae), David A. Andow, Steven G. Pueppke, Arthur W. Schaafsma, Aaron J. Gassmann, Thomas W. Sappington, Lance Meinke, Paul D. Mitchell, Terrance M. Hurley, Richard L. Hellmich, R. Pat Porter Jan 2015

Early Detection And Mitigation Of Resistance To Bt Maize By Western Corn Rootworm (Coleoptera: Chrysomelidae), David A. Andow, Steven G. Pueppke, Arthur W. Schaafsma, Aaron J. Gassmann, Thomas W. Sappington, Lance Meinke, Paul D. Mitchell, Terrance M. Hurley, Richard L. Hellmich, R. Pat Porter

Department of Entomology: Faculty Publications

Transgenic Bt maize that produces less than a high-dose has been widely adopted and presents considerable insect resistance management (IRM) challenges.Western corn rootworm, Diabrotica virgifera virgifera LeConte, has rapidly evolved resistance to Bt maize in the field, leading to local loss of efficacy for some corn rootworm Bt maize events. Documenting and responding to this resistance has been complicated by a lack of rapid diagnostic bioassays and by regulatory triggers that hinder timely and effective management responses. These failures are of great concern to the scientific and agricultural community. Specific challenges posed by western corn rootworm resistance to Bt maize, …


A Monographic Revision Of The Genus Hoplopyga Thomson, 1880 (Coleoptera: Scarabaeidae: Cetoniinae: Gymnetini), Jennifer M. Shaughney, Brett C. Ratcliffe Jan 2015

A Monographic Revision Of The Genus Hoplopyga Thomson, 1880 (Coleoptera: Scarabaeidae: Cetoniinae: Gymnetini), Jennifer M. Shaughney, Brett C. Ratcliffe

Department of Entomology: Faculty Publications

A comprehensive revision of the genus Hoplopyga Thomson, 1880 is presented. Eighteen species are redescribed, and two new species are described. A key for identification, distribution maps, and images of the 20 species are provided. Hoplopyga ruteri Antoine is removed from the genus and placed in junior synonymy with Macrocranius similis Schürhoff. Lectotypes are designated for Hoplopyga brasiliensis (Gory and Percheron), Hoplopyga corumbana Schürhoff, and Hoplopyga multipunctata (Gory and Percheron). The following new synonymies are established: Hoplopyga lucidiventris (Thomson) is Hoplopyga foeda (Schaum); Hoplopyga spurca (Janson) is Hoplopyga liturata (Olivier); Hoplopyga monacha (Gory and Percheron) and Hoplopyga rubida (Gory and …


Short-Horned Grasshopper Subfamilies Feed At Different Rates On Big Bluestem And Switchgrass Cultivars, Muhammad Ullah, Fatima Mustafa, William W. Hoback, Mathew L. Brust, Kerri M. Farnsworth-Hoback, John E. Foster Jan 2015

Short-Horned Grasshopper Subfamilies Feed At Different Rates On Big Bluestem And Switchgrass Cultivars, Muhammad Ullah, Fatima Mustafa, William W. Hoback, Mathew L. Brust, Kerri M. Farnsworth-Hoback, John E. Foster

Department of Entomology: Faculty Publications

Grasshopper species belonging to subfamilies Melanoplinae, Gomphocerinae and Oedipodinae were tested for their feeding rate on three types of grass. All grasshopper species were offered Shawnee and Kanlow cultivars of switchgrass, Panicum virgatum L. and big bluestem, Andropogon gerardii Vitman. The grasshoppers, Melanoplus femurrubrum and Melanoplus differentialis were also tested for their feeding on turgid or wilted leaves of the Shawnee cultivar of switchgrass. We found that M. differentialis consumed more switchgrass compared to big bluestem while M. femurrubrum and Arphia xanthoptera consumed the most Shawnee switchgrass. The M. differentialis consumed more turgid grass compared to wilted switchgrass. The feeding …


Genome-Wide Transcript Profiling Reveals Novel Breast Cancer-Associated Intronic Sense Rnas, Sang Woo Kim, Elane Fishilevich, Gustavo Arango-Argoty, Yuefeng Lin, Guodong Liu, Zhihua Li, A. Paula Monaghan, Mark Nichols, Bino John Jan 2015

Genome-Wide Transcript Profiling Reveals Novel Breast Cancer-Associated Intronic Sense Rnas, Sang Woo Kim, Elane Fishilevich, Gustavo Arango-Argoty, Yuefeng Lin, Guodong Liu, Zhihua Li, A. Paula Monaghan, Mark Nichols, Bino John

Department of Entomology: Faculty Publications

Non-coding RNAs (ncRNAs) play major roles in development and cancer progression. To identify novel ncRNAs that may identify key pathways in breast cancer development, we performed high-throughput transcript profiling of tumor and normal matched-pair tissue samples. Initial transcriptome profiling using high-density genome-wide tiling arrays revealed changes in over 200 novel candidate genomic regions that map to intronic regions. Sixteen genomic loci were identified that map to the long introns of five key protein-coding genes, CRIM1, EPAS1, ZEB2, RBMS1, and RFX2. Consistent with the known role of the tumor suppressor ZEB2 in the cancer-associated epithelial to mesenchymal transition (EMT), in situ …


Behavioural Responses Of Stable Flies To Cattle Manure Slurry Associated Odourants, K Tangtrakulwanich, T A. Albuquerque, G. J. Brewer, F. P. Baxendale, L Zurek, D N. Miller, D. B. Taylor, K A. Friesen, J. J. Zhu Jan 2015

Behavioural Responses Of Stable Flies To Cattle Manure Slurry Associated Odourants, K Tangtrakulwanich, T A. Albuquerque, G. J. Brewer, F. P. Baxendale, L Zurek, D N. Miller, D. B. Taylor, K A. Friesen, J. J. Zhu

Department of Entomology: Faculty Publications

Stable flies (Stomoxys calcitrans [Diptera: Muscidae] L.) are blood-feeding synanthropic pests, which cause significant economic losses in livestock. Stable fly antennae contain olfactory sensilla responsive to host and host environment-associated odours. Field observation indicated that the abundance of stable flies increased significantly in grasslands or crop fields when cattle manure slurry was applied. Major volatile compounds emanating from manure slurry were collected and identified. Behavioural responses of stable flies to those compounds were investigated in laboratory bioassays and field-trapping studies. Results from olfactometer assays revealed that phenol, p-cresol and m-cresol were attractive to adult stable flies. When …


Comparisons Of Antifeedancy And Spatialrepellency Of Three Natural Product Repellents Against Horn Flies, Haematobia Irritans (Diptera:Muscidae), Junwei J. Zhu, Gary J. Brewer, David J. Boxler, Kristina Friesen, David B. Taylor Jan 2015

Comparisons Of Antifeedancy And Spatialrepellency Of Three Natural Product Repellents Against Horn Flies, Haematobia Irritans (Diptera:Muscidae), Junwei J. Zhu, Gary J. Brewer, David J. Boxler, Kristina Friesen, David B. Taylor

Department of Entomology: Faculty Publications

BACKGROUND: Horn flies are among the most important biting fly pests of cattle in the United States. Horn fly management is largely dependent upon pesticides, which ultimately leads to the rapid development of insecticide resistance. Alternative control strategies, including repellents, have shown promising results in reducing fly biting. In the present study, we examined the efficacy and longevity of recently identified natural product repellents against horn flies.

RESULTS: Catnip oil, geraniol and C8910 acids reduced horn fly feeding in a laboratory bioassay and also exhibited spatial repellency in the olfactometer. Residual activity was observed for up to 3 days in …


A Protocol To Preserve The Integrity Of Stable Fly (Diptera: Muscidae) Dna For Long Distance Shipment, K. M. Kneeland, Steven R. Skoda, Elizabeth A. Claassen, John E. Foster Jan 2015

A Protocol To Preserve The Integrity Of Stable Fly (Diptera: Muscidae) Dna For Long Distance Shipment, K. M. Kneeland, Steven R. Skoda, Elizabeth A. Claassen, John E. Foster

Department of Entomology: Faculty Publications

Population genetic studies on a global scale may be hampered by the ability to acquire quality samples from distant countries. Preservation methods must be adequate to prevent samples from decay during shipping, so an adequate quantity of quality DNA can be extracted for analysis, and materials used must not be prohibited by the pertinent Postal Service. We tested a shipping protocol for stable flies which preserves the samples and adheres to Postal regulations. Samples were soaked in 95% ethanol for 6, 12, 24 and 48 hours; the ethanol was then poured off. Samples remained 'dry' for 2, 4, 6 and …


Assessing Chemical Control Of Earthworms At Airports, Thomas W. Seamans, Bradley F. Blackwell, Glen E. Bernhardt, Daniel A. Potter Jan 2015

Assessing Chemical Control Of Earthworms At Airports, Thomas W. Seamans, Bradley F. Blackwell, Glen E. Bernhardt, Daniel A. Potter

United States Department of Agriculture Wildlife Services: Staff Publications

Earthworms originating from Europe (e.g., Lumbricus spp., Aporrectodea spp.), which are common in the United States and southern Canada can create hazardous conditions at airports by attracting birds that pose a threat to aircraft. These nonnative earthworms are also considered pests on golf courses and sports fields, as well as having detrimental effects on temperate boreal forests. No toxicants or repellents are currently registered for earthworm control in the United States. Our purpose was to identify products that could be used to repel or suppress nonnative earthworms on airports or other managed sites where they pose a hazard or nuisance. …


Evolution Of Hematophagous Habit In Triatominae (Heteroptera: Reduviidae), Fernando Otálora-Luna, Antonio J. Pérez-Sánchez, Claudia Sandoval, Elis Aldana Jan 2015

Evolution Of Hematophagous Habit In Triatominae (Heteroptera: Reduviidae), Fernando Otálora-Luna, Antonio J. Pérez-Sánchez, Claudia Sandoval, Elis Aldana

Harold W. Manter Laboratory of Parasitology: Library Materials

All members of Triatominae subfamily (Heteroptera: Reduviidae), potential vectors of Trypanosoma cruzi, etiologic agent of the Chagas disease, feed on blood. Through evolution, these bugs have fixed special morphological, physiological, and behavioral aptations (adaptations and exaptations) adequate to feed on blood. Phylogeny suggests that triatomines evolved from predator reduvids which in turn descended from phytophagous hemipterans. Some pleisiomorphic traits developed by the reduvid ancestors of the triatomines facilitated and modeled hematophagy in these insects. Among them, mouthparts, saliva composition, enzymes, and digestive symbionts are the most noticeable. However, the decisive step that allowed the shift from predation to hematophagy …


Sequence-Characterized Amplified Regions That Differentiate New World Screwworms From Other Potential Wound-Inhabiting Flies, Joan A Christen, Steven R. Skoda, Tiffany Heng-Moss, Donald J. Lee, John E. Foster Jan 2015

Sequence-Characterized Amplified Regions That Differentiate New World Screwworms From Other Potential Wound-Inhabiting Flies, Joan A Christen, Steven R. Skoda, Tiffany Heng-Moss, Donald J. Lee, John E. Foster

Department of Entomology: Faculty Publications

Abstract. New World screwworms, Cochliomyia hominivorax (Coquerel, 1858), were once devastating pests of warmblooded animals in the United States before they were successfully eradicated using the sterile insect technique. Guarding against the introduction of screwworms to North America or any other screwworm-free area relies on rapid, reliable identification of suspected cases. In the current study, the DNA from excised markers generated by randomly amplified polymorphic DNA polymerase chain reaction was used as the basis to generate 2 species-specific sequence-characterized amplified region molecular markers. Resulting primer pairs, named CR92A1 and J1A2 (each with forward and reverse components), produced amplicons of 852 …


Transcriptional Profiling Of Resistant And Susceptible Buffalograsses In Response To Blissus Occiduus (Hemiptera: Blissidae) Feeding, Crystal M Ramm, Michael Wachholtz, Keenan Amunsen, Teresa J. Donze, Tiffany Heng-Moss, Paul Twigg, Nathan A. Palmer, Gautam Sarath, Frederick P. Baxendale Jan 2015

Transcriptional Profiling Of Resistant And Susceptible Buffalograsses In Response To Blissus Occiduus (Hemiptera: Blissidae) Feeding, Crystal M Ramm, Michael Wachholtz, Keenan Amunsen, Teresa J. Donze, Tiffany Heng-Moss, Paul Twigg, Nathan A. Palmer, Gautam Sarath, Frederick P. Baxendale

Department of Entomology: Faculty Publications

ABSTRACT Understanding plant resistance mechanisms at a molecular level would provide valuable insights into the biological pathways impacted by insect feeding, and help explain specific plant tolerance mechanisms. As a first step in this process, we conducted next-generation sequencing using RNA extracted from chinch bug-tolerant and -susceptible buffalograss genotypes at 7 and 14 d after chinch bug feeding. Sequence descriptions and gene ontology terms were assigned to 1,701 differentially expressed genes. Defense-related transcripts were differentially expressed within the chinch bug-tolerant buffalograss, Prestige, and susceptible buffalograss, 378. Interestingly, four peroxidase transcripts had higher basal expression in tolerant control plants compared with …


Susceptibility Of Nebraska Western Corn Rootworm (Coleoptera: Chrysomelidae) Populations To Bt Corn Events, David S. Wangila, Aaron J. Gassmann, Jennifer L. Petzold-Maxwell, B. Wade French, Lance J. Meinke Jan 2015

Susceptibility Of Nebraska Western Corn Rootworm (Coleoptera: Chrysomelidae) Populations To Bt Corn Events, David S. Wangila, Aaron J. Gassmann, Jennifer L. Petzold-Maxwell, B. Wade French, Lance J. Meinke

Department of Entomology: Faculty Publications

Transgenic plants have been widely adopted by growers to manage the western corn rootworm, Diabrotica virgifera virgifera LeConte, in field corn. Because of reduced efficacy in some Nebraska fields after repeated use of Cry3Bb1-expressing hybrids, single plant bioassays were conducted in 2012 and 2013 to characterize the susceptibility of western corn rootworm populations to the rootwormactive proteins Cry3Bb1, mCry3A, and Cry34/35Ab1. Results demonstrate that there are heritable differences in susceptibility of Nebraska western corn rootworm populations to rootworm-active Bt traits. Proportional survival and corrected survival data coupled with field histories collectively support the conclusion that a level of field resistance …


Transcriptional Analysis Of Susceptible And Resistant European Corn Borer Strains And Their Response To Cry1f Protoxin, Neetha Nanoth Vellichirammal, Haichuan Wang, Seong-Il Eyun, Estuko N. Moriyama, Brad Coates, Nicholas Miller, Blair Siegfried Jan 2015

Transcriptional Analysis Of Susceptible And Resistant European Corn Borer Strains And Their Response To Cry1f Protoxin, Neetha Nanoth Vellichirammal, Haichuan Wang, Seong-Il Eyun, Estuko N. Moriyama, Brad Coates, Nicholas Miller, Blair Siegfried

Department of Entomology: Faculty Publications

Background: Despite a number of recent reports of insect resistance to transgenic crops expressing insecticidal toxins from Bacillus thuringiensis (Bt), little is known about the mechanism of resistance to these toxins. The purpose of this study is to identify genes associated with the mechanism of Cry1F toxin resistance in European corn borer (Ostrinia nubilalis Hübner). For this, we compared the global transcriptomic response of laboratory selected resistant and susceptible O. nubilalis strain to Cry1F toxin. We further identified constitutive transcriptional differences between the two strains.

Results: An O. nubilalis midgut transcriptome of 36,125 transcripts was assembled de novo from …


Degree-Day Prediction Models For The Flight Phenology Of Western Bean Cutworm (Lepidoptera: Noctuidae) Assessed With The Concordance Correlation Coefficient, Anthony A. Hanson, Roger D. Moon, R. J. Wright, Thomas E. Hunt, William D. Hutchinson Jan 2015

Degree-Day Prediction Models For The Flight Phenology Of Western Bean Cutworm (Lepidoptera: Noctuidae) Assessed With The Concordance Correlation Coefficient, Anthony A. Hanson, Roger D. Moon, R. J. Wright, Thomas E. Hunt, William D. Hutchinson

Department of Entomology: Faculty Publications

Western bean cutworm, Striacosta albicosta (Smith) (Lepidoptera: Noctuidae), is a native, univoltine pest of corn and dry beans in North America. The current degree-day model for predicting a specified percentage of yearly moth flight involves heat unit accumulation above 10°C after 1 May. However, because the moth’s observed range has expanded into the northern and eastern United States, there is concern that suitable temperatures before May could allow for significant S. albicosta development. Daily blacklight moth catch and temperature data from four Nebraska locations were used to construct degree-day models using simple or sine-wave methods, starting dates between 1 January …


Estimation Of Efficacy Functions For Products Used To Manage Corn Rootworm Larval Injury, N A. Tinsley, P. D. Mitchell, Robert Wright, Lance J. Meinke, R. E. Estes, M. E. Gray Jan 2015

Estimation Of Efficacy Functions For Products Used To Manage Corn Rootworm Larval Injury, N A. Tinsley, P. D. Mitchell, Robert Wright, Lance J. Meinke, R. E. Estes, M. E. Gray

Department of Entomology: Faculty Publications

Maize, Zea mays L., is an economically important crop grown throughout the world. Corn rootworm, Diabrotica spp. (Coleoptera: Chrysomelidae), larvae constitute a significant economic threat to maize production in the United States, where yield losses and management costs associated with corn rootworm species exceed $1 billion annually. Furthermore, the introduction of the western corn rootworm, D. virgifera virgifera LeConte, into maize-producing regions of Europe has made managing corn rootworm larval injury an international concern. Larvae injure maize plants by feeding on root tissue and are the primary target of management activities. Products commonly used to protect root systems from injury …


Diel Patterns Of Colaspis Brunnea And Colaspis Crinicornis (Coleoptera: Chrysomelidae) In Southeastern Nebraska, Kentaro Miwa, Lance Meinke Jan 2015

Diel Patterns Of Colaspis Brunnea And Colaspis Crinicornis (Coleoptera: Chrysomelidae) In Southeastern Nebraska, Kentaro Miwa, Lance Meinke

Department of Entomology: Faculty Publications

A field study was conducted to increase our understanding of diel activity patterns of Colaspis brunnea (F.) and Colaspis crinicornis Schaeffer (Coleoptera: Chrysomelidae) in key crop habitats. Within 24-h periods, C. brunnea was sampled in clover fields (primarily red clover, Trifolium pretense (L.), with some sweet clover, Melilotus officinalis (L.) Pallas, and downy brome, Bromus tectorum (L.)) and soybean, Glycine max (L.) Merrill, fields, using a sweep-net, while whole-plant-count sampling was used to monitor C. crinicornis densities in field corn, Zea mays (L.). Sweep-net captures of C. brunnea were significantly greater at night than during the day, suggesting possible vertical …


Developmental Biology And Effects Of Adult Diet On Consumption, Longevity, And Fecundity Of Colaspis Crinicornis (Coleoptera: Chrysomelidae), Kentaro Miwa, Lance Meinke Jan 2015

Developmental Biology And Effects Of Adult Diet On Consumption, Longevity, And Fecundity Of Colaspis Crinicornis (Coleoptera: Chrysomelidae), Kentaro Miwa, Lance Meinke

Department of Entomology: Faculty Publications

The chrysomelid beetle Colaspis crinicornis Schaeffer (Coleoptera: Chrysomelidae) occurs primarily in the Great Plains region of the United States. Little is known about the biology and ecology of this species, but over the last decade, it has become increasingly common in the corn, Zea mays L., and soybean, Glycine max (L.) Merrill, agroecosystem of southeastern Nebraska. As part of a larger comprehensive project to understand the natural history and pest potential of this species, laboratory experiments were conducted to study the developmental biology, morphological characters of immature stages, and the effect of adult diet on consumption, longevity, and fecundity. Females …


Quantitative Trait Locus Mapping And Functional Genomics Of An Organophosphate Resistance Trait In The Western Corn Rootworm, Diabrotica Virgifera Virgifera, B. S. Coates, A. P. Alves, H. Wang, X. Zhou, T. Nowatzki, H. Chen, M. Rangasamy, H. M. Robertson, C. W. Whitfield, K. K. Walden, Stephen D. Kachman, B. W. French, L. J. Meinke, D. Hawthorne, C. A. Abel, T. W. Sappington, B. D. Siegfried, N. J. Miller Jan 2015

Quantitative Trait Locus Mapping And Functional Genomics Of An Organophosphate Resistance Trait In The Western Corn Rootworm, Diabrotica Virgifera Virgifera, B. S. Coates, A. P. Alves, H. Wang, X. Zhou, T. Nowatzki, H. Chen, M. Rangasamy, H. M. Robertson, C. W. Whitfield, K. K. Walden, Stephen D. Kachman, B. W. French, L. J. Meinke, D. Hawthorne, C. A. Abel, T. W. Sappington, B. D. Siegfried, N. J. Miller

Department of Entomology: Faculty Publications

The western corn rootworm, Diabrotica virgifera virgifera, is an insect pest of corn and population suppression with chemical insecticides is an important management tool. Traits conferring organophosphate insecticide resistance have increased in frequency amongst D. v. virgifera populations, resulting in the reduced efficacy in many corn-growing regions of the USA. We used comparative functional genomic and quantitative trait locus (QTL) mapping approaches to investigate the genetic basis of D. v. virgifera resistance to the organophosphate methyl-parathion. RNA from adult methyl-parathion resistant and susceptible adults was hybridized to 8331 microarray probes. The results predicted that 11 transcripts were significantly up-regulated in …


Ethylene Contributes To Maize Insect Resistance1-Mediated Maize Defense Against The Phloem Sap-Sucking Corn Leaf Aphid, Joe Louis, Saumik Basu, Suresh Varsani, Lina Castano-Duque, Victoria Jiang, W. Paul Williams, Gary W. Felton, Dawn S. Luthe Jan 2015

Ethylene Contributes To Maize Insect Resistance1-Mediated Maize Defense Against The Phloem Sap-Sucking Corn Leaf Aphid, Joe Louis, Saumik Basu, Suresh Varsani, Lina Castano-Duque, Victoria Jiang, W. Paul Williams, Gary W. Felton, Dawn S. Luthe

Department of Entomology: Faculty Publications

Signaling networks among multiple phytohormones fine-tune plant defense responses to insect herbivore attack. Previously, it was reported that the synergistic combination of ethylene (ET) and jasmonic acid (JA) was required for accumulation of the maize insect resistance1 (mir1) gene product, a cysteine (Cys) proteinase that is a key defensive protein against chewing insect pests in maize (Zea mays). However, this study suggests that mir1-mediated resistance to corn leaf aphid (CLA; Rhopalosiphum maidis), a phloem sap-sucking insect pest, is independent of JA but regulated by the ET-signaling pathway. Feeding by CLA triggers the rapid accumulation of mir1 transcripts in the resistant …


Morphology And Proteome Characterization Of The Salivary Glands Of The Western Chinch Bug (Hemiptera: Blissidae), Crystal Ramm, Astri Wayadande, Lisa Baird, Renu Nandakumar, Nandakumar Madayiputhiya, Keenan Amundsen, Teresa Donze-Reiner, Frederick Baxendale, Gautam Sarath, Tiffany Heng-Moss Jan 2015

Morphology And Proteome Characterization Of The Salivary Glands Of The Western Chinch Bug (Hemiptera: Blissidae), Crystal Ramm, Astri Wayadande, Lisa Baird, Renu Nandakumar, Nandakumar Madayiputhiya, Keenan Amundsen, Teresa Donze-Reiner, Frederick Baxendale, Gautam Sarath, Tiffany Heng-Moss

Department of Entomology: Faculty Publications

The western chinch bug, Blissus occiduus Barber, is a serious pest of buffalograss, Buchloe dactyloides (Nuttall) due to physical and chemical damage caused during the feeding process. Although previous work has investigated the feeding behaviors of chinch bugs in the Blissus complex, no study to date has explored salivary gland morphology and the associated salivary complex of this insect. Whole and sectioned B. occiduus salivary glands were visualized using light and scanning electron microscopy to determine overall structure and cell types of the salivary glands and their individual lobes. Microscopy revealed a pair of trilobed principal glands and a pair …


Transcriptional Responses Of Tolerant And Susceptible Soybeans To Soybean Aphid (Aphis Glycines Matsumura) Herbivory, Travis J. Prochaska, Teresa Donze-Reiner, L. Marchi-Werle, N. A. Palmer, Thomas E. Hunt, Gautam Sarath, Tiffany Heng-Moss Jan 2015

Transcriptional Responses Of Tolerant And Susceptible Soybeans To Soybean Aphid (Aphis Glycines Matsumura) Herbivory, Travis J. Prochaska, Teresa Donze-Reiner, L. Marchi-Werle, N. A. Palmer, Thomas E. Hunt, Gautam Sarath, Tiffany Heng-Moss

Department of Entomology: Faculty Publications

The soybean aphid, Aphis glycines Matsumura, was introduced in 2000 to North America and has become one of the most significant pests to soybean, Glycine max (L.) Merrill, production. Possible solutions to this problem are the use of resistant plants and the understanding of the genes involved in plant resistance. In this study, we sought to better understand the genes involved in the tolerance response of soybean plants to the soybean aphid, utilizing tolerant (KS4202) and susceptible (K-03-4686) plants. Studies were conducted under greenhouse conditions. Leaf samples of both tolerant and susceptible plants were collected at day 5 and day …


Impact Of Wheat Streak Mosaic Virus And Triticum Mosaic Virus Coinfection Of Wheat On Transmission Rates By Wheat Curl Mites, C. Oliveira-Hofman, Stephen N. Wegulo, Satyanarayana Tatineni, G. L. Hein Jan 2015

Impact Of Wheat Streak Mosaic Virus And Triticum Mosaic Virus Coinfection Of Wheat On Transmission Rates By Wheat Curl Mites, C. Oliveira-Hofman, Stephen N. Wegulo, Satyanarayana Tatineni, G. L. Hein

Department of Entomology: Faculty Publications

Wheat streak mosaic virus (WSMV) and Triticum mosaic virus (TriMV) are transmitted by the wheat curl mite (WCM, Aceria tosichella), and coinfections of wheat by these viruses are common in the field. Previous work has shown that mite genotypes vary in their ability to transmit TriMV. However, the degree to which coinfection of wheat modifies WCM vector competence has not been studied. The objective was to determine whether mite genotypes differed in virus transmission ability when feeding on wheat coinfected by WSMV and TriMV. First, WCM genotype type 2 was used to determine virus transmission rates from mock-, WSMV-, …


Planting Date And Variety Selection For Management Of Viruses Transmitted By The Wheat Curl Mite (Acari: Eriophyidae), Anthony J. Mcmechan, Gary Hein Jan 2015

Planting Date And Variety Selection For Management Of Viruses Transmitted By The Wheat Curl Mite (Acari: Eriophyidae), Anthony J. Mcmechan, Gary Hein

Department of Entomology: Faculty Publications

Wheat is an important food grain worldwide, and it is the primary dryland crop in the western Great Plains. A complex of three viruses (Wheat streak mosaic, Wheat mosaic, and Triticum mosaic viruses) is a common cause of loss in winter wheat production in the Great Plains. All these viruses are transmitted by the wheat curl mite (Aceria tosichella Keifer). Once these viruses are established, there are no curative actions; therefore, prevention is the key to successful management. A study was designed to evaluate preventative management tactics (planting date, resistant varieties) for reducing the impact from this virus complex. …


On-Plant Larval Movement And Feeding Behavior Of Fall Armyworm (Lepidoptera: Noctuidae) On Reproductive Corn Stages, Luiz E. R. Pannuti, Edson L. L. Baldin, Thomas E. Hunt, Silvana L. Vieira De Paula Moraes Jan 2015

On-Plant Larval Movement And Feeding Behavior Of Fall Armyworm (Lepidoptera: Noctuidae) On Reproductive Corn Stages, Luiz E. R. Pannuti, Edson L. L. Baldin, Thomas E. Hunt, Silvana L. Vieira De Paula Moraes

Department of Entomology: Faculty Publications

Spodoptera frugiperda J.E. Smith (fall armyworm) is considered one of the most destructive pests of corn throughout the Americas. Although this pest has been extensively studied, little is known about its larval movement and feeding behavior on reproductive compared to vegetative corn stages. Thus, we conducted studies with two corn stages (R1 and R3) and four corn plant zones (tassel, above ear, ear zone, and below ear) in the field at Concord, NE (USA), and in the field and greenhouse at Botucatu, SP (Brazil), to investigate on-plant larval movement. The effects of different corn tissues (opened tassel, …