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International Centre of Insect Physiology and Ecology

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Articles 991 - 1020 of 1129

Full-Text Articles in Entomology

Selection Of Virulent Isolates Of Entomopathogenic Hyphomycetes Against Clavigralla Tomentosicollis Stål. And Evaluation In Cage Experiment Using Three Cowpea Varieties, S. Ekesi Dec 1999

Selection Of Virulent Isolates Of Entomopathogenic Hyphomycetes Against Clavigralla Tomentosicollis Stål. And Evaluation In Cage Experiment Using Three Cowpea Varieties, S. Ekesi

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The virulence of 8 isolates of entomopathogenic hyphomycetes against adult and 5th instar nymph of Clavigralla tomentosicollis was evaluated in the laboratory at 4 different concentrations of inoculum. At all concentrations, Beauveria bassiana CPD 9 and Metarhizium anisopliae CPD 5 caused the highest mortality in adult bug ranging from 58 to 97% and 53 to 100%, respectively at 7 days post inoculation. The same isolates had the shortest LT50 (3.5 and 4.1 days, respectively) and the lowest LC50 (1.8 × 105 and 9.8 × 104 conidia ml-1) values in adult insects. In nymphs, M. anisopliae CPD 5 was the most …

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Infochemicals In Mosquito Host Selection: Human Skin Microflora And Plasmodium Parasites, Marieta A.H. Braks, Robert A. Anderson, Bart G.J. Knols Oct 1999

Infochemicals In Mosquito Host Selection: Human Skin Microflora And Plasmodium Parasites, Marieta A.H. Braks, Robert A. Anderson, Bart G.J. Knols

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The interaction between the African malaria vector Anopheles gambiae and its human host has traditionally been viewed within a bitrophic context, considering only the human and the mosquito. Recently, the influence of the Plasmodium parasite on the interaction has been recognized, because it affects the physiology and/or behaviour of humans and mosquitoes. However, studies on odour-mediated host-seeking behaviour of An. gambiae and other Diptera have provided evidence that a fourth group of organisms should be taken into consideration. Human skin microflora play a role in the production of odorous compounds that might function as kairomones for mosquitoes. Here, Marieta Braks, …

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Cleome Hirta Essential Oil As Livestock Tick (Rhipicephalus Appendiculatus) And Maize Weevil (Sitophilus Zeamais) Repellent, M. W. Ndungu, S. C. Chhabra, W. Lwande Oct 1999

Cleome Hirta Essential Oil As Livestock Tick (Rhipicephalus Appendiculatus) And Maize Weevil (Sitophilus Zeamais) Repellent, M. W. Ndungu, S. C. Chhabra, W. Lwande

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The repellency of the essential oil of the shrub Cleome hirta and of three identified constituents (phytol, (+)-cedrol, n-octacosane) was evaluated against the livestock tick, Rhipicephalus appendiculatus and the maize weevil, Sitophillus zeamais. In a tick climbing repellency bioassay, the oil exhibited repellency which, at the highest dose, was comparable to that of the commercial arthropod repellent N,N-diethyltoluamide (DEET). In a Y-tube olfactometer bioassay, the oil showed higher or comparable repellency against S. zeamais relative to DEET at all the doses tested. The potential of C. hirta in livestock tick and maize weevil control is discussed. Copyright (C) 1999 Elsevier …

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The Effects Of A Tsetse Dna Virus Infection On The Functions Of The Male Accessory Reproductive Gland In The Host Fly Glossina Morsitans Centralis (Diptera; Glossinidae), Rosemary C. Sang, Walter G.Z.O. Jura, Leonard H. Otieno, Richard W. Mwangi, Peter Ogaja Jun 1999

The Effects Of A Tsetse Dna Virus Infection On The Functions Of The Male Accessory Reproductive Gland In The Host Fly Glossina Morsitans Centralis (Diptera; Glossinidae), Rosemary C. Sang, Walter G.Z.O. Jura, Leonard H. Otieno, Richard W. Mwangi, Peter Ogaja

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Freshly deposited third instar Glossina morsitans centralis larvae were infected with the tsetse DNA virus by microinjection, and at emergence adult males were separated from the females and fed on rabbit blood every second day for 8 days. A control group treated with sterile saline were handled similarly. They were dissected, and comparative observations made on the appearance and size of the accessory reproductive glands (ARG) in infected and control males. Regularly fed 8-day-old males from infected and control groups were mated to 2-day-old normal females obtained from the insectay. After separation from copula, the females were dissected and the …

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Isolation Of Trypanosoma Brucei From The Monitor Lizard (Varanus Niloticus) In An Endemic Focus Of Rhodesian Sleeping Sickness In Kenya, Zipporah Njagu, Steve Mihok, Elizabeth Kokwaro, Didier Verloo Mar 1999

Isolation Of Trypanosoma Brucei From The Monitor Lizard (Varanus Niloticus) In An Endemic Focus Of Rhodesian Sleeping Sickness In Kenya, Zipporah Njagu, Steve Mihok, Elizabeth Kokwaro, Didier Verloo

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Monitor lizards were sampled along the shores of Lake Victoria to detect natural infections of potentially human-infective trypanosomes. In an area with endemic rhodesian sleeping sickness, one of 19 lizards was infected (Busia, Kenya). Six of ten lizards also showed indirect evidence of infection with Trypanosoma brucei (antibody ELISA). In an area with no recent history of human disease (Rusinga Island), no parasites were found and no antibodies to T. brucei were detected. The isolate was identified as T. brucei through xenodiagnosis (completion of the life cycle in the salivary glands of tsetse), and through molecular techniques (positive reactions with …

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Trypanosome Infection Rate In Cattle At Nguruman, Kenya, R. A. Tarimo-Nesbitt, T. K. Golder, R. D. Dransfield, M. F. Chaudhury, R. Brightwell Feb 1999

Trypanosome Infection Rate In Cattle At Nguruman, Kenya, R. A. Tarimo-Nesbitt, T. K. Golder, R. D. Dransfield, M. F. Chaudhury, R. Brightwell

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Trypanosome infection rate in cattle at Nguruman was investigated in a study conducted in 1984-1986. Shifting pastoralism significantly reduced trypanosome infections in cattle. The cattle were more heavily infected with Trypanosoma congolense (16.5%) than Trypanosoma vivax (4.95%) and Trypanosoma brucei (0.19%). Trypanosoma theileri was observed only once among the cattle examined. Mixed trypanosome infections in cattle were observed to be 2.75% and 0.014% for T. congolense/T. vivax and T. congolense/T. brucei, respectively. The duration of infection in the cattle was 55 days for T. congolense and 79 days for T. vivax. High infections in cattle were observed 2 months after …

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Insights For The Management Of Different Locust Species From New Findings On The Chemical Ecology Of The Desert Locust, Ahmed Hassanali, Magzoub Omer Bashir Jan 1999

Insights For The Management Of Different Locust Species From New Findings On The Chemical Ecology Of The Desert Locust, Ahmed Hassanali, Magzoub Omer Bashir

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Recent studies on the chemical ecology of the desert locust, Schistocerca gregaria, have unravelled a rich and complex system of pheromonal and other semiochemical-mediated communication in this insect. The key pheromones associated with such behavioural processes as social cohesion and communal egg-laying have been characterised. The semiochemical bases of such physiological processes as maturation of solitarious insects before the arrival of seasonal rains, development of gregarious traits in crowded solitarious insects, development of maturation synchrony in gregarious insects, and maternal transfer of gregarious characters to the offspring are better understood. Desert locust semiochemicals are finding two principal uses: as tools …

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Comparative Evaluation Of Cotesia Flavipes And C. Sesamiae (Hymenoptera: Braconidae) For The Management Of Chilo Partellus (Lepidoptera: Pyralidae) In Kenya, M. N. Sallam, W. A. Overholt, E. Kairu Jan 1999

Comparative Evaluation Of Cotesia Flavipes And C. Sesamiae (Hymenoptera: Braconidae) For The Management Of Chilo Partellus (Lepidoptera: Pyralidae) In Kenya, M. N. Sallam, W. A. Overholt, E. Kairu

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Cotesia flavipes Cameron, a gregarious larval endoparasitoid native to the Indo-Australian region, was imported from Pakistan and released in Kenya in 1993 for management of the exotic stemborer, Chilo partellus (Swinhoe). Recent reports confirmed the successful establishment of the parasitoid in three locations in Kenya and in northern Tanzania. Functional response studies on this parasitoid and an indigenous congener, Cotesia sesamiae (Cameron), indicated that C. flavipes had a higher searching ability and attacked more larvae when Chilo partellus was the host. When a native stemborer, Sesamia calamistis Hampson, was the host, there was no significant difference between numbers attacked by …

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Evidence For Additional Pheromonal Components Mediating Oviposition Aggregation In Schistocerca Gregaria, Baldwyn Torto, Yousif O.H. Assad, Peter G.N. Njagi, Ahmed Hassanali Jan 1999

Evidence For Additional Pheromonal Components Mediating Oviposition Aggregation In Schistocerca Gregaria, Baldwyn Torto, Yousif O.H. Assad, Peter G.N. Njagi, Ahmed Hassanali

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Oviposition responses of gregarious adult gravid females of the desert locust Schistocerca gregaria to sand previously used for oviposition by gregarious females (contaminated sand, with eggs and froth removed) and to sterilized sand (noncontaminated sand) were compared in bioassays. Gregarious females preferentially oviposited into contaminated sand. The number of egg pods laid by the females was found to correlate with the frequency of use of the contaminated sand for oviposition. Charcoal-trapped volatiles from the contaminated sand also elicited similar responses from gregarious females. Gas chromatography-electroantennographic detection (GC-EAD) analysis of the trapped volatiles revealed the presence of three electrophysiologically active compounds …

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Diapause Termination Of Chilo Partellus (Swinhoe) And Chilo Orichalcociliellus Strand (Lepidoptera: Pyralidae), V. C. Ofomata, W. A. Ovekholt, R. I. Egwuatu Jan 1999

Diapause Termination Of Chilo Partellus (Swinhoe) And Chilo Orichalcociliellus Strand (Lepidoptera: Pyralidae), V. C. Ofomata, W. A. Ovekholt, R. I. Egwuatu

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The intensity of diapause of field-collected diapausing larvae of Cliilo partcllus (Swinhoe) and Cliilo oriclmlcocilielltis Strand (Lepidoptera: Pyrnlidae) was compared in the laboratory at 28 ± 0.5°C under a photopcriod of 12:12 (L:D)h. Chilo partcllus terminated diapause faster than C. oriclmlcociliellus. Mean time to diapause termination was 9.7 days for C. parlellus and 14.4 days for C. oriclinlcociliellns. Male and female C. parlellns larvae terminated diapause and adults emerged at the same time, while significant differences were found in diapause termination and in emergence times between male and female C. orichnlcocilk'llits. © 1999 ICIPE.

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Spatial Distribution And Habitat Characterization Of Anopheline Mosquito Larvae In Western Kenya, Noboru Minakawa, Clifford M. Mutero, John I. Githure, John C. Beier, Guiyun Yan Jan 1999

Spatial Distribution And Habitat Characterization Of Anopheline Mosquito Larvae In Western Kenya, Noboru Minakawa, Clifford M. Mutero, John I. Githure, John C. Beier, Guiyun Yan

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Studies were conducted to characterize larval habitats of anopheline mosquitoes and to analyze spatial heterogeneity of mosquito species in the Suba District of western Kenya. A total of 128 aquatic habitats containing mosquito larvae were sampled, and 2,209 anopheline and 10,538 culicine larvae were collected. The habitats were characterized based on size, pH, distance to the nearest house and to the shore of Lake Victoria, coverage of canopy, surface debris, algae and emergent plants, turbidity, substrate, and habitat types. Microscopic identification of third and fourth-instar anopheline larvae did not yield any Anopheles funestus or other anophelines. A total of 829 …

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Responses Of Glossina Fuscipes Fuscipes (Diptera: Glossinidae) And Other Diptera To Carbon Dioxide In Linear And Dense Forests, M. M. Mohamed-Ahmed, S. Mihok Jan 1999

Responses Of Glossina Fuscipes Fuscipes (Diptera: Glossinidae) And Other Diptera To Carbon Dioxide In Linear And Dense Forests, M. M. Mohamed-Ahmed, S. Mihok

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The responses of Glossina fuscipes fuscipes Newstead and other Diptera to carbon dioxide were studied in linear and dense forests along the shores of Lake Victoria, Kenya. Flies were caught in biconical traps and were intercepted with electric nets while in flight near traps. Carbon dioxide dispensed at a high rate (51 min-1) in linear forest failed to increase the numbers of tsetse attracted to or caught in traps. In contrast, catches of non-biting Muscidae, Stomoxyinae and Tabanidae were improved by up to 11 times. Inside dense forest, carbon dioxide released at half this rate increased both the numbers of …

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Intra- And Interspecific Aggregation Responses Of Locusta Migratoria Migratorioides And Schistocerca Gregaria And A Comparison Of Their Pheromone Emissions, Abdoulaye Niassy, Baldwyn Torto, Peter G.N. Njagi, Ahmed Hassanali, Daniel Obeng-Ofori, Jonathan N. Ayertey Jan 1999

Intra- And Interspecific Aggregation Responses Of Locusta Migratoria Migratorioides And Schistocerca Gregaria And A Comparison Of Their Pheromone Emissions, Abdoulaye Niassy, Baldwyn Torto, Peter G.N. Njagi, Ahmed Hassanali, Daniel Obeng-Ofori, Jonathan N. Ayertey

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Gregarious nymphs of Schistocerca gregaria and Locusta migratoria migratorioides are often seen to march together in common bands in the field. In the present study, the intraspecific aggregation responses of nymphal and older stages of the two insects were compared with their interspecific responses. Unlike S. gregaria, L. m. migratorioides shows a lesser pattern of stage and sex differentiation in its aggregation pheromone biology. Thus, although fifth-instar nymphs did not respond significantly to the adult pheromone, adults responded significantly to the nymph pheromone. No cross- stage aggregative responses occur in S. gregaria. In the adults of S. gregaria, production of …

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Development Of Odour-Baited Traps For Glossina Swynnertoni (Diptera: Glossinidae), P. N. Ndegwa, S. Mihok Jan 1999

Development Of Odour-Baited Traps For Glossina Swynnertoni (Diptera: Glossinidae), P. N. Ndegwa, S. Mihok

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Three new prototype traps, (S1-S3), were developed during studies of the behavioural ecology of Glossina swynnertoni Austen in Kenya and Tanzania. The traps were compared in latin square experiments relative to the regular biconical trap as a standard and a selection of other conventional tsetse traps. Observations were also made on fly behaviour in the vicinity of traps using electric nets and sticky materials. When baited with acetone and 1-octen-3-ol, the S1 trap was 3.5 times as effective in catching G. swynnertoni in Kenya as the biconical trap. In Tanzania, the relative performance of the S1 and biconical traps differed; …

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Potential Of The Entomopathogenic Fungus, Metarhizium Anisopliae (Metsch.) Sorokin For Control Of The Legume Flower Thrips, Megalurothrips Sjostedti (Trybom) On Cowpea In Kenya, S. Ekesi, N. K. Maniania, K. Ampong-Nyarko, I. Onu Nov 1998

Potential Of The Entomopathogenic Fungus, Metarhizium Anisopliae (Metsch.) Sorokin For Control Of The Legume Flower Thrips, Megalurothrips Sjostedti (Trybom) On Cowpea In Kenya, S. Ekesi, N. K. Maniania, K. Ampong-Nyarko, I. Onu

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Field experiments were conducted at Mbita, western Kenya for two seasons to evaluate the potential of the entomopathogenic fungus, Metarhizium anisopliae (Metsh.) Sorokin, for biological control of the legume flower thrips, Megalurothrips sjostedti (Trybom), on cowpea. An ultra-low-volume (ULV) oil/aqueous formulation and a high-volume (HV) aqueous formulation of conidia were applied three times each at two concentrations of 1 x 1011 and 1 x 1013 conidia ha-1. Compared with the untreated controls, both formulations significantly reduced thrips populations and plant damage in both seasons. Adult insects collected from fungal-treated plots and reared in the laboratory showed a high mortality due …

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Evaluation Of Mortality Factors And Risk Analysis For The Design Of An Integrated Pest Management System, Olivier Roux, Johann Baumgärtner Jun 1998

Evaluation Of Mortality Factors And Risk Analysis For The Design Of An Integrated Pest Management System, Olivier Roux, Johann Baumgärtner

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A probabilistic approach of survival analysis based on the theory of competing risks was applied to the potato tuber moth Phthorimaea operculella (Zeller) affecting tubers in Tunisian rustic stores. Exponential curve of death, i.e. constant force of mortality functions were used in a case study on a cohort time basis. The additive property of crude cause-specific forces of mortality is used to combine mortality factors, which permits to judge different control strategies. In an integrated pest management perspective, the effect of basic mortalities, i.e innate and dispersal related mortalities is recombined with native natural enemies. Thereafter, we calculate the force …

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Electrophoretic And Phylogenetic Analyses Of Selected Allopatric Populations Of The Cotesia Flavipes Complex (Hymenoptera: Braconidae), Parasitoids Of Cereal Stemborers, Susan W. Kimani-Njogu, William A. Overholt, James B. Woolley, Charles O. Omwega Apr 1998

Electrophoretic And Phylogenetic Analyses Of Selected Allopatric Populations Of The Cotesia Flavipes Complex (Hymenoptera: Braconidae), Parasitoids Of Cereal Stemborers, Susan W. Kimani-Njogu, William A. Overholt, James B. Woolley, Charles O. Omwega

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Electrophoretic studies were conducted on the Cotesia flavipes Cameron complex and Cotesia glomerata L. (Hymenoptera: Braconidae). Esterase, glucosephosphate isomerase, hexokinase, sorbital dehydrogenase and phosphogluconate dehydrogenase had fixed alleles for the three species and could be used to distinguish them. Cotesia chilonis (Matsumura) and Cotesia sesamiae (Cameron), have a genetic identity value characteristic of distinct species (Nei's genetic identity, I = 0.587). Cladistic analysis of the allozyme data indicated that allopatric populations currently included in C. flavipes may not be a monophyletic group.

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Short Report: Influence Of Centers For Disease Control Light Trap Position, Relative To A Human-Baited Bed Net, On Catches Of Anopheles Gambiae And Culex Quinquefasciatus In Tanzania, Leonard E.G. Mboera, Japhet Kihonda, Marieta A.H. Braks, Bart G.J. Knols Jan 1998

Short Report: Influence Of Centers For Disease Control Light Trap Position, Relative To A Human-Baited Bed Net, On Catches Of Anopheles Gambiae And Culex Quinquefasciatus In Tanzania, Leonard E.G. Mboera, Japhet Kihonda, Marieta A.H. Braks, Bart G.J. Knols

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The best position for Centers for Disease Control (Atlanta, GA) light traps, in relation to human-occupied bed nets for trapping of host-seeking Anopheles gambiae Giles and Culex quinquefasciatus Say mosquitoes, was determined in Tanzania. Significantly higher catches were recorded for both species when the trap was positioned at the foot end of the bed, near the top of the net. Parity rates were significantly higher near the top of the net than at the level of the host. Since trap position affects the catch size and the proportion of infectious mosquitoes therein, standardized use of this sampling technique for estimating …

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Evidence Of Antibiosis Resistance To The Spotted Stemborer, Chilo Partellus (Swinhoe) In A Maize (Zea Mays L.) Population, C. J.M. Mutinda, K. N. Saxena, S. O. Ajala, P. O. Aviecho Jan 1998

Evidence Of Antibiosis Resistance To The Spotted Stemborer, Chilo Partellus (Swinhoe) In A Maize (Zea Mays L.) Population, C. J.M. Mutinda, K. N. Saxena, S. O. Ajala, P. O. Aviecho

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S2 line and S2 test-cross recurrent selection procedures were initiated to improve the resistance of a maize (Zen mays L.) population to the spotted stemborer, Chllo partclltis (Swinhoe) (Lepidoptera: Pyralidae). Thirty-five selections developed from the population were evaluated for resistance to the stemborer in western Kenya. Resistance was rated according to leaf feeding (LF), stem tunnelling (ST) and deadheart formation (DH) following artificial infestation with first-instar C. pnrtcUus. Larval establishment and growth were also assessed. The reduction in foliar damage ratings, percentage stem length tunnelled, and percentage of plants showing deadheart after one cycle of recurrent selection were 4.38 %, …

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Cost Of Tsetse Trapping Using The Ng2g Trap: A Case Study In Kenya, George Tombe Lako Jan 1998

Cost Of Tsetse Trapping Using The Ng2g Trap: A Case Study In Kenya, George Tombe Lako

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In a study to determine the costs of tsetse trapping in terms of construction materials, labour, transport and maintenance, two hundred forty-six traps used to control Glossina pallidipes in an area of 100 square kilometres at Nguruman, Kenya were evaluated from November 1990 to July 1992. The total cost of materials, labour and servicing per trap per year, was US$ 30.6 which, at that time was equivalent to 1071 Kenya shillings. ©1998ICIPE.

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Effect Of Species, Age, And Sex Of Tsetse On Response To Infection By Metarhizium Anisopliae, Nguya K. Maniania, Adedapo Odulaja Jan 1998

Effect Of Species, Age, And Sex Of Tsetse On Response To Infection By Metarhizium Anisopliae, Nguya K. Maniania, Adedapo Odulaja

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Laboratory studies were carried out to determine the effect of sex and age on the susceptibility of tsetse, Glossina morsitans morsitans and G. m. centralis, to the entomopathogenic fungus, Metarhizium anisopliae. Both species of host flies were susceptible to fungal infection. Female flies were generally more susceptible than male flies. Three host ages (40, 20, and <1 day-old) were used; the youngest group was most resistant to fungal infection. Interactions between species, sex and age were significant on many occasions. Age usually accounted for the largest variability in mortality, followed by sex. All flies of age 40 days died between 7 and 8 days after infection whereas some of the younger flies, especially age 0, lived longer than 10 days. Log10 day probit (LDP) mortality regressions fitted well to most of the data sets. LDP slopes were significant and high, ranging between 4.3 and 12.8, indicating a generally high mortality rate of increase over days. The slopes differed significantly between species, sexes, and ages, but grouping by age was more intra homogeneous than by species or sex. The 50% lethal time mortalities (LT50) ranged between 4 and 7 days for age 0, 3 and 6 days for age 20, and about 5 days for age 40. Corresponding ranges of the LT95 were 8 to 20, 5 to 10, and 6 to 7 days for ages 0, 20 and 40, respectively. The significance of these results in the fungal disease transmission by tsetse is discussed.

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Brood Guarding Behavior And Life History Characteristics Of Goniozus Indicus Ashmead (Hymenoptera: Bethylidae), A Larval Ectoparasitoid Of Lepidopteran Stemborers, Keiji Takasu, William A. Overholt Jan 1998

Brood Guarding Behavior And Life History Characteristics Of Goniozus Indicus Ashmead (Hymenoptera: Bethylidae), A Larval Ectoparasitoid Of Lepidopteran Stemborers, Keiji Takasu, William A. Overholt

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Brood guarding behavior and life history characteristics of Goniozus indicus Ashmead (Hymenoptera: Bethylidae) were examined in the laboratory. Longevity was increased when females were provided host frass, and females reared in association with hosts lived longer than females maintained separately from hosts. Females that were allowed free access to hosts oviposited at intervals of one to eight days and never parasitized more than one host per day. Females laid 5-25 eggs per host, and life time fecundity was 15-52 eggs at 25°C, 7-60 eggs at 28°C, and 5-25 eggs at 31°C. Females exhibited egg guarding behavior, remaining with the hosts …

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The Effects Of A Dna Virus Infection On The Reproductive Otential Of Female Tsetse Flies, Glossina Morsitans Centralis And Glossina Morsitans Morsitans (Diptera: Glossinidae), Rosemary C. Sang, Walter G.Z.O. Jura, Leonard H. Otieno, Richard W. Mwangi Jan 1998

The Effects Of A Dna Virus Infection On The Reproductive Otential Of Female Tsetse Flies, Glossina Morsitans Centralis And Glossina Morsitans Morsitans (Diptera: Glossinidae), Rosemary C. Sang, Walter G.Z.O. Jura, Leonard H. Otieno, Richard W. Mwangi

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Reproductive anomalies associated with the tsetse DNA virus infection in the female tsetse hosts, Glossina morsitans centralis Machado and Glossina morsitans morsitans Westwood, inoculated with the virus during the 3rd instar larval stage were studied and the data compared to those obtained from the control females injected with sterile physiological saline. Virus infected flies had significantly longer first and second pregnancy cycles (P<0.0001) and produced pupae that were of significantly less weight in milligrams (P<-0.0001) compared to controls. Transmission of the virus to progeny was not absolute and only 21% of G. m. centralis and 48% of G. m. morsitans first progeny flies from infected females developed salivary gland hypertrophy as a result of transmission from mother to progeny. The virus infected females produced significantly fewere pupae compared to the controls during the experimental period (P<0.00001).

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Fat Body Triacylglycerol Lipase In Solitary And Gregarious Phases Of Schistocerca Gregaria (Forskal) (Orthoptera: Acrididae), Dorington O. Ogoyi, Ellie O. Osir, Norah K. Olembo Jan 1998

Fat Body Triacylglycerol Lipase In Solitary And Gregarious Phases Of Schistocerca Gregaria (Forskal) (Orthoptera: Acrididae), Dorington O. Ogoyi, Ellie O. Osir, Norah K. Olembo

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The activities of the fat body triacylglycerol lipase were determined using 14C-trioleoylglycerol. Resting activities were estimated at 1.98 ± 0.24 and 1.95 ± 0.53 nmol free fatty acid (FFA)/hr/mg protein for gregarious and solitary locusts, respectively. Administration of adipokinetic hormone (AKH) I led to the activation of lipase with peak activities occurring after 30 min. In the solitary locusts, activities of 2.28 ± 0.16 and 2.30 ± 0.43 nmol FFA/hr/mg were obtained following administration of 10 and 2 pmol AKH I, respectively. In the gregarious locusts, enzyme activities of 3.17 ± 0.66 and 2.47 ± 0.39 nmol FFA/hr/mg were obtained …

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Suitability Of Busseola Fusca And Sesamia Calamistis (Lepidoptera: Noctuidae) For The Development Of Two Populations Of Cotesia Sesamiae (Hymenoptera: Braconidae) In Kenya, A. J. Ngi-Song, W. A. Overholt, R. Stouthamer Jan 1998

Suitability Of Busseola Fusca And Sesamia Calamistis (Lepidoptera: Noctuidae) For The Development Of Two Populations Of Cotesia Sesamiae (Hymenoptera: Braconidae) In Kenya, A. J. Ngi-Song, W. A. Overholt, R. Stouthamer

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The suitability of Sesamia calamistis Hampson and Busseola fusca (Fuller) for the development of two geographical populations of Cotesia sesamiae (Cameron) was examined in the laboratory. One population of the parasitoid was collected from the coast of Kenya and the other from the inland. Both populations of the parasitoid could develop on S. calamistis. On B. fusca, the inland population of C. sesamiae was able to develop, while the population from the coastal area of Kenya was encapsulated. Mating studies revealed that the two parasitoid populations were partially reproductively isolated. Unidirectional incompatibility, possibly caused by the Wolbachia infection, was observed …

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The Magnitude Of Site And Time Interaction Effect In Tsetse Fly (Diptera: Glossinidae) Trap Catches, A. Odulaja, S. Mihok, I. M. Abu-Zinid Jan 1998

The Magnitude Of Site And Time Interaction Effect In Tsetse Fly (Diptera: Glossinidae) Trap Catches, A. Odulaja, S. Mihok, I. M. Abu-Zinid

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Site and time effects are important factors determining trap catches of tsetse flies. These factors may interact significantly and therefore confound interpretation of time series data used for population monitoring. We therefore investigated the magnitude and importance of site x time interactions in trap catches of Glossina pallidipes Austen and G. longipennis Corti using a 2200 trap-days (400 trap-months) data set. The interaction was found to be significant (P < 0.05) in 46-100% of the combinations of different numbers of months and sites between 2 and 12. The mean percent variance due to the interaction ranged between 4% and 28% for G. pallidipes and 12% and 36% for G. longipennis. The interaction was usually less important than the effect of site alone but more important than the effect of time alone. These results suggest that tsetse researchers should examine critically the adequacy of existing approaches to population monitoring with traps and to testing new traps and odour baits.

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Olfactory Responses Of Glossina Fuscipes Fuscipes (Diptera: Glossinidae) To The Monitor Lizard Varanus Niloticus Niloticus, M. M. Mohamed-Ahmed Jan 1998

Olfactory Responses Of Glossina Fuscipes Fuscipes (Diptera: Glossinidae) To The Monitor Lizard Varanus Niloticus Niloticus, M. M. Mohamed-Ahmed

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Visual and olfactory responses of Glossina fuscipes fuscipes Newstead to the monitor lizard, Varanus niloticus niloticus Laurenti were studied using various catching devices near Lake Victoria, Kenya. Electric nets baited with visible lizards caught more males (x2.1) and significantly more females (x2.0) than unbaited nets. Lizards concealed in electrified black Pvc pipe models, simulating the shape and size of a monitor lizard, increased significantly the catches of tsetse by 2.1 times. Fresh lizard urine dispensed at an evaporation rate of 500 to 1000 mg/h also increased significantly the catches of flies at biconical traps, electrified models and electric nets. Identification …

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Comparison Of Nest Structure And Caste Parameters Of Sympatric Species Of Odontotermes (Termitidae, Macrotermitinae) In Kenya, J. P.E.C. Darlington Dec 1997

Comparison Of Nest Structure And Caste Parameters Of Sympatric Species Of Odontotermes (Termitidae, Macrotermitinae) In Kenya, J. P.E.C. Darlington

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Four species of Odontotermes are common in semi-arid grassland around Kajiado. They all build centralised nests consisting of a cluster of discrete chambers housing the fungus combs. In at least one nest of each species all the chambers were measured and mapped, and the fungus combs extracted, dried and weighed. Three species, O. monodon, O. anceps and O. tanganicus, build 'classical' nests with open vertical shafts that link up underground, apparently acting as a ventilation system. The fourth species O. zambesiensis has no open shafts and no ventilation system. Each of the four species has fungus combs that are distinctly …

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Effects Of Juvenile Hormone Treatment On Phase Changes And Pheromone Production The Desert Locust, Schistocerca Gregaria (Forskal) (Orthoptera: Acrididae), Amer I. Tawfik, Ellie O. Osir, Ahmed Hassanali, Sayed H. Ismail Nov 1997

Effects Of Juvenile Hormone Treatment On Phase Changes And Pheromone Production The Desert Locust, Schistocerca Gregaria (Forskal) (Orthoptera: Acrididae), Amer I. Tawfik, Ellie O. Osir, Ahmed Hassanali, Sayed H. Ismail

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The roles of juvenile hormone III (JH III) on phase changes and pheromone production were examined in laboratory-reared gregarious desert locust, Schistocerca gregaria (Forskal). The hormone was applied to 5th instar nymphs and newly emerged adult locusts. Generally, the 5th instar nymphs exhibited a higher sensitivity to hormone treatments than the adults. Hormone applications inhibited pheromone production (as measured by the amounts of phenylacetonitrile released). In addition, JH III had a significant effect on the external colouration and absorbance ratios of the haemolymph pigments. It is concluded that the effects of exogenous JH III on gregarious locusts represent a shift …

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Effects Of Tsetse Dna Virus Infection On The Survival Of A Host Fly,Glossina Morsitans Centralis(Diptera; Glossinidae), Rosemary C. Sang, Walter G.Z.O. Jura, Leonard H. Otieno, Peter M. Tukei, Richard W. Mwangi May 1997

Effects Of Tsetse Dna Virus Infection On The Survival Of A Host Fly,Glossina Morsitans Centralis(Diptera; Glossinidae), Rosemary C. Sang, Walter G.Z.O. Jura, Leonard H. Otieno, Peter M. Tukei, Richard W. Mwangi

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Freshly deposited third instarGlossina morsitans centralislarvae were infected with the tsetse DNA virus by microinjection. At emergence comparative observations were made on longevity and feeding behavior of infected and control flies. Gut tissues from the control and virus-infected flies were fixed and processed for light and electron microscopy. The longevity of infected flies was significantly reduced compared to that of the controls (P<0.05). The main mortality factors in the virus-infected flies with severe lesions in the salivary glands were starvation due to failure to feed and clotting of blood in and/or rupture of the crop. Rupture of the midgut also caused some mortalities. Infected flies probed significantly more times during feeding to repletion (P<0.05) and took significantly longer to feed compared to the control flies (P<0.05). Infected flies which fed took significantly less blood compared to the controls (P<0.05). Histological studies revealed pathological changes in the epithelial cells of the anterior midgut secretory midgut and the posterior midgut. There was severe disintegration of the membranous organelles, especially the mitochondria and rough endoplasmic reticulum, leading to extensive vacuolation in such epithelial cells. No viral particles were observed in the secretory and posterior midgut. Virions were observed in the anterior midgut lumen and occasional particles were seen invading the epithelial cells in this area of the midgut, especially in heavily infected flies. © 1997 Academic Press.

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