Entomology Commons^™

Genetic Differentiation Of Western Corn Rootworm Populations (Coleoptera: Chrysomelidae) Relative To Insecticide Resistance, Hong Chen, Haichuan Wang, Blair D. Siegfried

Department of Entomology: Faculty Publications

Abstract

As the single most important pest of field corn, Zea mays L., throughout most of the Corn Belt, the western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), has undergone repeated selection for resistance to a variety of insecticides that persist widely among Nebraska populations. In this study, we used 11 microsatellite markers to genotype two populations with high levels of resistance to methyl-parathion and aldrin (Polk and Stromsburg), two populations with low and intermediate levels of resistance (Mead and Clay Center) from Nebraska, and one population from outside the Corn Belt (Safford, Arizona). The genetic diversity measured by …

Bean Leaf Beetle (Coleoptera: Chrysomelidae) And Bean Pod Mottle Virus In Soybean: Biology, Ecology, And Management, Buyung A. R. Hadi, Jeffrey D. Bradshaw, Marlin E. Rice, John J. Hill

Department of Entomology: Faculty Publications

Bean leaf beetle, Cerotoma trifurcata (Förster), is a pest of soybean found in many production areas in the United States. The bean leaf beetle larvae feed on soybean root nodules, whereas the adults feed on the above ground parts of soybean such as cotyledon, leaves, and pods. Bean leaf beetle is also a very efficient vector of Bean pod mottle virus, a widespread virus of soybean in the south and southeastern United States with recent expansion into the north central region of the country. This article summarizes bean leaf beetle biology, ecology, and its impact on soybean production in the …

Oviposition Induced Volatile Emissions From African Smallholder Farmers' Maize Varieties, Amanuel Tamiru, Toby J.A. Bruce, Charles A.O. Midega, Christine M. Woodcock, Michael A. Birkett, John A. Pickett, Zeyaur R. Khan

All Peer-Reviewed Publications

Maize (corn), Zea mays, is a genetically diverse crop, and we have recently shown that certain open pollinated varieties (OPVs) of Latin American origin possess a trait not present in mainstream commercial varieties: they produce volatiles in response to stemborer oviposition that are attractive to stemborer parasitoids. Here, we tested whether a similar tritrophic effect occurs in the African OPVs 'Nyamula' and 'Jowi'. Herbivore induced plant volatiles (HIPVs) were collected from plants exposed to egg deposition by the stemborer Chilo partellus. In a four-arm olfactometer bioassay, the parasitic wasp Cotesia sesamiae preferred samples containing HIPVs from plants with eggs to …

Nebline, February 2012

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Letter From The Dean, Michael Vayda

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Distribution Of Genes And Repetitive Elements In The Diabrotica Virgifera Virgifera Genome Estimated Using Bac Sequencing, Brad S. Coates, Analiza P. Alves, Haichuan Wang, Kimberly K. O. Walden, B. Wade French, Nicholas J. Miller, Craig A. Abel, Hugh M. Robertson, Thomas W. Sappington, Blair D. Siegfried

Department of Entomology: Faculty Publications

Feeding damage caused by the western corn rootworm, Diabrotica virgifera virgifera, is destructive to corn plants in North America and Europe where control remains challenging due to evolution of resistance to chemical and transgenic toxins. A BAC library, DvvBAC1, containing 109,486 clones with 104 ± 34.5 kb inserts was created, which has an ~4.56X genome coverage based upon a 2.58 Gb (2.80 pg) flow cytometry-estimated haploid genome size. Paired end sequencing of 1037 BAC inserts produced 1.17Mb of data (~0.05% genome coverage) and indicated ~9.4 and 16.0% of reads encode, respectively, endogenous genes and transposable elements (TEs). …

Discovery: The Student Journal Of Dale Bumpers College Of Agricultural, Food And Life Sciences - Volume 13 2012, Several Authors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Biology Department Newsletter, No.2, Sacred Heart University

Biology Newsletter

No abstract provided.

Nebline, January 2012

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: UNL Food Processing Center Provides Valuable Assistance to Food Manufacturers

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2011 Annual Report Special Pullout Section

Contents, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Instructions For Authors, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Invertebrate Abundances And Diversity Of A Six Year Old Organic Apple Orchard In Northwest Arkansas, Cory Johnson, Brina Smith, Mary C. Savin

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Ground cover mulch applications in perennial systems can have multiple benefits, one of which may be to enhance the size and diversity of the ground surface faunal community. To determine if ground cover and organic fertilizer applications altered invertebrate communities, litters in an experimental 0.4-ha organic apple orchard in Fayetteville, Ark. were sampled during a four week period beginning in February 2012. The orchard was planted in 2006 in a replicated 4 × 3 factorial design with organic ground cover and fertilizer treatments applied annually each April. Invertebrates were extracted using Berlese funnels and hand sorting techniques. Ground covers (wood …

Larval Survival And Plant Injury Of Cry1ab-Susceptible, -Resistant, And -Heterozygous Genotypes Of The Sugarcane Borer On Transgenic Corn Containing Single Or Pyramided Bt Genes, David S. Wangila, B. Rogers Leonard, Yaoyu Bai, Graham P. Head, Fangneng Huang

Department of Entomology: Faculty Publications

Transgenic corn (Zea mays L.) products expressing multiple Bt proteins targeting a same group of insect pests have become commercially available in the U.S. The first commercialized pyramided Bt corn technologies for managing lepidopteran pests include Genuity® VT Triple Pro™ and Genuity® SmartStax™. In this study, larval survival and plant injury of Cry1Ab-susceptible (Cry1Ab-SS), -resistant (Cry1Ab-RR), and -heterozygous (Cry1Ab-RS) genotypes of the sugarcane borer, Diatraea saccharalis F., on five commercial corn hybrids were evaluated in a leaf tissue bioassay and two greenhouse trials during 2010–2011. The five hybrids included two non-Bt corn and three Bt corn hybrids representing three …

Mortality Impact Of Mon863 Transgenic Maize Roots On Western Corn Rootworm Larvae In The Field, T. L. Clark, D. L. Frank, B. W. French, Lance J. Meinke, D. Moellenbeck, T. T. Vaughn, B. E. Hibbard

Department of Entomology: Faculty Publications

Mortality of western corn rootworm (Diabrotica virgifera virgifera LeConte) due to feeding on MON863 transgenic maize (Zea mays L.) expressing the Cry3Bb1 protein was evaluated at three Missouri sites in both 2003 and 2004 and at one site each in South Dakota, Nebraska, and Iowa in 2004. To do this, survivorship relative to survivorship on isoline maize (i.e., the same genetic background, but without Cry3Bb1) was evaluated. Comparisons were made using low (1650–2500 eggs/m) and high (3300–3500 eggs/m) western corn rootworm egg densities. Significantly fewer beetles were recovered from MON863 than from isoline maize. Emergence from MON863 as …

Using Sex Pheromone Traps In The Decision-Making Process For Pesticide Application Against Fall Armyworm (Spodoptera Frugiperda [Smith] [Lepidoptera: Noctuidae]) Larvae In Maize, Ivan Cruz, Maria De Lourdes Corrêa Figueiredo, Rafael Braga Da Silva, Ivana Fernandes Da Silva, Cristiane De Souza Paula, John E. Foster

Department of Entomology: Faculty Publications

The fall armyworm, Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae), is a major pest of maize and frequently demands control measures. The timing of insecticide application is a key factor in determining its efficiency, so an experiment was designed to investigate this. Application of insecticide was based on three criteria: (i) the number of trap-caught moths in a Delta-type trap with a commercial sex pheromone lure placed in the center of the target area, soon after plant emergence; (ii) the percentage of plants exhibiting pinhole-type damage (10% or 20%), and (iii) the percentage of plants exhibiting shot hole–type damage (10% or 20%) …

Susceptibility Of Cry1ab Maize-Resistant And -Susceptible Strains Of Sugarcane Borer (Lepidoptera: Crambidae) To Four Individual Cry Proteins, Liping Zhang, Fangneng Huang, B. Rogers Leonard, Mao Chen, Thomas Clark, Yu Cheng Zhu, David S. Wangila, Fei Yang, Ying Niu

Department of Entomology: Faculty Publications

Sugarcane borer, Diatraea saccharalis (F.), is a major target of Bt maize in South America and many areas of the US mid-south region. Six laboratory strains of D. saccharalis were established from six single-pair F2 families possessing major resistance alleles to Cry1Ab maize hybrids. Susceptibility of the six strains was evaluated on diet treated with each of four purified trypsin-activated Bt proteins, Cry1Ab, Cry1Aa, Cry1Ac, and Cry1F. Bt susceptibility of the six strains was compared with that of known Cry1Ab-susceptible and -resistant strains of D. saccharalis. At least two of the six strains demonstrated a similar level (>526-fold) …

Nebline, November/December 2011

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

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Screenhouse Transmission Of Beauveria Bassiana From Infected To Non-Infected Adults Of Banana Weevil, C. A. Omukoko, J. M. Wesonga, E. M. Kahangi, L. S. Wamocho, K. N. Maniania

All Peer-Reviewed Publications

Laboratory studies have revealed great potential of entomopathogenic fungus Beauveria bassiana for use against the banana weevil, Cosmopolites sordidus, in banana. Adult banana weevils exposed to wet conidia of Beauveria bassiana suffered high mortalities ranging from 50-70% by 40 days post exposure when a concentration of 1×109 was used in the laboratory with three isolates ICIPE 273, M313 and M207 which were selected on the basis of high virulence against C. sordidus and high sporulation. However, impractical field delivery methods and high costs associated with the application of B. bassiana against C. sordidus prevent its use and commercialisation in banana …

Management Of Alien Invasive Insect Pest Species And Diseases Of Fruits And Vegetables: Experiences From East Africa, B. Nyambo, S. Sevgan, A. Chabi-Olaye, S. Ekesi

All Peer-Reviewed Publications

The East African region is currently struggling to cope with several alien invasive insect pest species (AIS): the diamondback moth (DBM), fruit flies, Liriomyza leafminers, thrips and spider mites to name just a few. These insect pests have wide host range, are difficult to control with synthetic pesticides, and most are of quarantine status. AIS are becoming frequent pests in horticulture production systems, threat to food security, market access and rural livelihoods. The impact of these pests is aggravated by variability in climatic conditions, few taxonomic expertise for accurate and timely identification of new species, inadequate knowledge on viable alternative …

Horticultural Pest Management And The African Economy: Successes, Challenges And Opportunities In A Changing Global Environment, S. Ekesi, A. Chabi-Olaye, S. Subramanian, C. Borgemeister

All Peer-Reviewed Publications

In many African countries, horticulture is the fastest growing agricultural sub-sector contributing significantly to national incomes. Diverse vegetables, fruits and spices are grown for domestic and export markets. Horticultural exports in Africa has increased by more than 50% in recent years and currently contributes >20% of agricultural exports by volume and income generation. Despite the growth, production has not kept pace with increasing demand due largely to biotic constraints attributed to arthropod pests. Agricultural research aimed at reducing insect pest damage can lead to increased horticultural production and improved rural livelihoods. icipe in collaboration with National Agricultural Research and Extension …

Nebline, October 2011

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

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Life History Of An Exotic Soft Scale Insect Phalacrococcus Howertoni (Hemiptera: Coccidae) Found In Florida, Kaushalya G. Amarasekare, Catharine M. Mannion

Agricultural and Environmental Sciences Faculty Research

We investigated the life history of an exotic soft scale insect, Phalacrococcus howertoni Hodges and Hodgson that damages croton and several other economically important ornamental and fruit plants in Florida. There was no difference in the development, survival, and reproduction of this scale insect species when reared either on croton or on buttonwood at 27 ± 1°C, 12:12 (L:D) h and 65% R. H. The scale insect practiced ovoviviparity. We observed that eggs that came out from vulva hatched to first instars immediately. Newly hatched nymphs had a tendency stay underneath the female body for somewhat less than approximately 24 …

Nebline, September 2011

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

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Nebline, August 2011

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

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Lancaster County Super Fair Schedule & Map Special Pullout Section

Nebline, July 2011

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Urban Agriculture is a Growing Trend

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Nebline, June 2011

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Lower Platte South Natural Resources District Trails in Lancaster County Offer Recreation & Education

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Nebline, May 2011

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Nature, Gardening & Science Come Together in the Butterfly Garden

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Nebline, April 2011

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Supplemental Kits Make Learning Nutrition Fun

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Nebline, March 2011

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Connect, Learn, and Share With Extension

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Weed Awareness Insert

Crop Updates 2011 - Pests And Diseases, Jeff Russell, Paul Umina, Svetlana Micic, Laura Fagan, Mark Seynour, Tony Dore, Pam Burgess, John Majewski, Manisha Shankar, Rob Loughman

Crop Updates

This session covers four papers from different authors:

1. Grains biosecurity – everyone’s business, Jeff Russell, Department of Agriculture and Food

2. Control of insect and mite pests in grains – insecticide resistance and integrated pest management (IPM), Paul Umina¹, Svetlana Micic² and Laura Fagan³, ¹CESAR and The University of Melbourne, ²Department of Agriculture and Food, ³University of Western Australia

3. Effect of cropping rotations on pest mites of broadacre agriculture, Svetlana Micic, Mark Seymour, Tony Dore and Pam Burgess, Department of Agriculture and Food

4. Common bunt resistance in …