Entomology Commons^™

Prospects For Repellent In Pest Control: Current Developments And Future Challenges, Emilie Deletre, Bertrand Schatz, Denis Bourguet, Fabrice Chandre, Livy Williams, Alain Ratnadass, Thibaud Martin

All Peer-Reviewed Publications

The overall interest in environmentally safe pest control methods and the rise of insecticide resistance in pest populations have prompted medical and agricultural entomology research on insect repellents in recent years. However, conducting research on repellent is challenging for several reasons: (1) the different repellent phenomena are not well defined; (2) it is difficult to test for and quantify repellent; (3) the physiological mechanisms are poorly known; (4) the field efficacy appears to be highly variable. Here, we identified five different types of repellent: expellency, irritancy, deterrency, odor masking and visual masking, and described behavioral bioassays to differentiate between them. …

Vector Competence Of Aedes Aegypti In Transmitting Chikungunya Virus: Effects And Implications Of Extrinsic Incubation Temperature On Dissemination And Infection Rates, Sophiah Mbaika, Joel Lutomiah, Edith Chepkorir, Francis Mulwa, Christopher Khayeka-Wandabwa, Caroline Tigoi, Elijah Oyoo-Okoth, James Mutisya, Zipporah Ng'ang'a, Rosemary Sang

All Peer-Reviewed Publications

Background: Aedes aegypti is a competent arthropod vector of chikungunya virus (CHIKV). The rate at which the virus disseminate in the vector is limited by temperature of their environment which can be an important determinant of geographical and seasonal limits to transmission by the arthropods in the tropics. This study investigated the vector competence of Ae. aegypti for CHIKV at ambient temperature of 32 and 26 °C (Coastal and Western Kenya respectively) reared at Extrinsic Incubation Temperature (EIT) of 32 and 26 °C that resembles those in the two regions. Methods: Ae. aegypti eggs were collected from coastal and Western …

Dynamics Of Two Pathogens In A Single Tick Population, Alexis White

Biology and Medicine Through Mathematics Conference

No abstract provided.

Can Climate-Driven Change Influence Silicon Assimilation By Cereals And Hence The Distribution Of Lepidopteran Stem Borers In East Africa?, P. A. Calatayud, E. Njuguna, S. Mwalusepo, M. Gathara, G. Okuku, A. Kibe, B. Musyoka, D. Williamson, G. Ong'amo, G. Juma, T. Johansson, S. Subramanian, E. Gatebe, B. Le Ru

All Peer-Reviewed Publications

In East Africa, lepidopteran stemborers such as Chilo partellus and Busseola fusca are major constraints to production of maize, which is the main staple food crop in the region. Cereals depend on silicon (Si)-based defences to fight off herbivores. Using altitudinal ranges in the East African highlands as ecological surrogates for inferring climate change, it was shown that Si concentrations in soil and maize decreased with altitude. This was attributed, in part, to low temperatures at high altitudes, which negatively affected Si assimilation by maize. Experiments showed that B. fusca was more susceptible to Si than C. partellus. Hence the …

Relationships Between Nutrients, Periphyton Abundance, And Benthic Macroinvertebrate Scrapers In Ozark Highland Streams, Harrison Smith

Biological Sciences Undergraduate Honors Theses

We collected samples from seven Arkansas streams during the months of August 2014 and February 2015 with the objective of determining possible relationships between nutrients, periphyton abundance and benthic macroinvertebrates. We hypothesized that periphyton would be positively associated with nutrients, and that increases in periphyton biomass would increase the abundance and diversity of benthic scraper families. Samples were collected from three transects at each site. The periphyton was analyzed for carbon, nitrogen, phosphorus, and ash free dry mass and Chl-a content. Macroinvertebrates families belonging to the feeding group of grazers/scrapers were compared with water column and periphyton nutrient concentrations, as …

Export Of Insect Ecosystem Services From Hayed Native Prairie, Wayne J. Ohnesorg

Department of Entomology: Dissertations, Theses, and Student Research

Natural areas, such as prairie, have the potential to provide the benefits of pest suppression and pollination for agricultural production. In Nebraska, prairie is often used for hay production. The impact of prairie hay production management practices on beneficial arthropods is not well understood. Four prairie hay meadows adjacent to row crop fields were selected in northeast Nebraska. The goal was to assess the impact of haying them on beneficial arthropods and the movement of natural enemies into adjacent crop fields. Three management practices were evaluated for hay harvested from prairie meadows one cut per growing season, two cuts per …

Effect Of Varied Disturbance Types On Dorylus (Anomma) Molestus Defensive Behavior In The West Usumbara Mountains, Tanzania., Ryan Mahar

Independent Study Project (ISP) Collection

The purpose of this study was to observe novel nest characteristics of Dorylus molestus (Siafu), as well as their defensive behavior in response to varied disturbance methods. The number of defensive soldiers at a specific section of a column was counted before and after each agitation. Varied agitation methods were used on columns without larvae presence to test how soldiers responded to different disturbance types. Disturbances on columns with larvae presence were compared to those on columns without larvae. Nest distribution, behavior, composition, and size were observed objectively. Mechanical disturbance was found to incite the greatest increase in defensive soldier …

Advances In Solid Phase Microextraction For The Analysis Of Volatile Compounds In Explosives, Tire Treatments, And Entomological Specimens, William D. Kranz

Open Access Dissertations

Solid phase microextraction is a powerful and versatile technique, well-suited to the analysis of numerous samples of forensic interest. The exceptional sensitivity of the SPME platform, combined with its adaptability to traditional GC-MS systems and its ability to extract samples with minimal work-up, make it appropriate to applications in forensic laboratories.

In a series of research projects, solid phase microextraction was employed for the analysis of explosives, commercial tire treatments, and entomological specimens. In the first project, the volatile organic compounds emanating from two brands of pseudo-explosive training aids for use in detector dog imprinting were determined by SPME-GC-MS, and …

Effects Of Environmental Factors On The Abundance Of Blacklegged Ticks, Jasmine L. Miller, Roger A. Lebrun, Howard S. Ginsberg

Senior Honors Projects

The nymphal stage of the blacklegged tick, Ixodes scapularis, is the major vector of Lyme disease, the most common vector-borne disease in North America. Tick abundance has generally been estimated using either flag/drag samples or samples from hosts. However, the biases of these sampling methods have not been adequately studied. We compared samples using both methods from sites in Massachusetts and Wisconsin. Tick abundance was compared with variables related to weather (temperature, relative humidity, and tick adverse moisture events), vegetation (canopy cover, tree density, shrub density, ground vegetation, and leaf litter cover), and host abundance (mice, small mammals, medium …

Visual, Vibratory, And Olfactory Cues Affect Interactions Between The Red Spider Mite Tetranychus Evansi And Its Predator Phytoseiulus Longipes, Ginette Yèyinou Azandémè-Hounmalon, Baldwyn Torto, Komi Kouma Mokpokpo Fiaboe, Sevgan Subramanian, Serge Kreiter, Thibaud Martin

All Peer-Reviewed Publications

Phytoseiulus longipes Evans (Mesostigmata: Phytoseiidae) is an exotic predator widely used in biological control programs for the red spider mite Tetranychus evansi Baker & Pritchard (Acari: Tetranychidae) in East Africa. However, little is known about the cues mediating this prey/predator interaction. Here, we performed behavioral assays to test the involvement of visual, vibratory, and olfactory cues using a combination of dead/living insects enclosed in either perforated or non-perforated transparent/opaque capsules. We monitored insect responses with a video tracking system and analyzed the data with Ethovision software. Our results showed avoidance behavior of T. evansi in the presence of live P. …

Approximating Optimal Release In A Deterministic Model For The Sterile Insect Technique, Sergio Ramirez, Luis F. Gordillo

Mathematics and Statistics Faculty Publications

Cost/benefit analyses are essential to support management planning and decisions before launching any pest control program. In particular, applications of the sterile insect technique (SIT) are often prevented by the projected economic burden associated with rearing processes. This has had a deep impact on the technique development and its use on insects with long larval periods, as often seen in beetles. Under the assumptions of long adult timespan and multiple mating, we show how to find approximate optimal sterile release policies that minimize costs. The theoretical framework proposed considers the release of insects by pulses and finds approximate optimal release …

Spatial Analysis Of Forest Crimes In Mark Twain National Forest, Missouri, Karun Pandit, Eddie Bevilacqua, Giorgos Mountrakis, Robert W. Malmsheimer

Journal of Geospatial Applications in Natural Resources

Forest crime mitigation has been identified as a challenging issue in forest management in the United States. Knowledge of the spatial pattern of forest crimes would help in wisely allocating limited enforcement resources to curb forest crimes. This study explores the spatial pattern of three different types of forest crimes: fire crime, illegal timber logging crime, and occupancy use crime in the Salem-Patosi Ranger District of Mark Twain National Forest. Univariate and bivariate Ripley’s K-functions were applied to explore the spatial patterns in crime events, like clustering and attraction among forest crime types. Results reveal significant clustering for each forest …

Comparison Of Terrain Indices And Landform Classification Procedures In Low-Relief Agricultural Fields, Derek A. Evans, Karl W. J. Williard, Jon E. Schoonover

Journal of Geospatial Applications in Natural Resources

Landforms control the spatial distribution of numerous factors associated with agronomy and water quality. Although curvature and slope are the fundamental surface derivatives used in landform classification procedures, methodologies for landform classifications have been performed with other terrain indices including the topographic position index (TPI) and the convergence index (CI). The objectives of this study are to compare plan curvature, the convergence index, profile curvature, and the topographic position index at various scales to determine which better identifies the spatial variability of soil phosphorus (P) within three low relief agricultural fields in central Illinois and to compare how two methods …

Discordant Data And Interpretation Of Results From Wildlife Habitat Models, Anita T. Morzillo, Michael G. Wing, Justin Long

Journal of Geospatial Applications in Natural Resources

Wildlife habitat management is an important part of natural resource management. As a result, there are a large number of models and tools for wildlife habitat assessment. A consequence of the many assessment tools is inconsistency when comparing results between tools, which may lead to potential confusion management decisions. Our objective was to compare results from two wildlife habitat models – one being relatively coarse (HUC5) scale and not spatially dynamic and the other being finer scale spatial data based on a 30 m spatial resolution –for habitat assessment of three species across the West Cascades of Oregon: Northern spotted …

Spiders From Multiple Functional Guilds Are Exposed To Bt-Endotoxins In Transgenic Corn Fields Via Prey And Pollen Consumption, Julie A. Peterson, John J. Obrycki, James D. Harwood

Department of Entomology: Faculty Publications

A comprehensive assessment of risk to natural enemies from Bt-endotoxins from bioengineered crops must evaluate potential harm, as well as exposure pathways in non-target arthropod food webs. Despite being abundant generalist predators in agricultural fields, spiders (Araneae) have often been overlooked in the context of Bt crop risk assessment. Spiders and their prey were collected from transgenic corn fields expressing lepidopteran-specific Cry1Ab, coleopteran-specific Cry3Bb1, both proteins, and a non-transgenic near isoline. Spiders and prey were screened for Cry1Ab and Cry3Bb1 using qualitative enzyme-linked immunosorbent assay. Spiders from the three most common functional guilds, wandering sheet-tangle weavers, orb-weavers, and ground runners, …

Role Of Microsatellite Markers In Molecular Population Genetics Of Fruit Flies With Emphasis On The Bactrocera Dorsalis Invasion Of Africa, Fathiya M. Khamis, Anna R. Malacrida

All Peer-Reviewed Publications

Microsatellites, also referred to as short tandem repeats (STRs) or simple sequence repeats (SSRs) are short sequences of tandem repeats of 1-6 bp in length in clusters of less than 150 bp flanked by sections of non-repetitive unique sequences that are scattered throughout the nuclear genome. These markers are co-dominant and hypervariable, revealing many alleles per locus; they are inherited in Mendelian fashion making them useful for detecting genetic variability within species. Once isolated and characterized, microsatellites can also be used in closely related taxa. Microsatellites can be amplified, even from highly degraded DNA, and are very simple to score. …

Integrated Management Of Fruit Flies-Case Studies From Uganda, Brian E. Isabirye, Caroline K. Nankinga, Alex Mayamba, Anne M. Akol, Ivan Rwomushana

All Peer-Reviewed Publications

Fruit flies (Diptera: Tephritidae) pose a threat to commercialisation of the horticulture industry in Uganda. They impair the quality and quantity of fruits produced, and limit access to lucrative regional and global markets. Here we explore past and present efforts, and future plans for research and management, of fruit flies in Uganda. Early research geared towards collection and identification of fruit flies recognised the pest status of many species and highlighted the need for establishing sustainable management strategies. Subsequently large-scale research initiatives have substantially increased knowledge on the biology and ecology of fruit flies in Uganda. Based on these studies, …

Intrinsic Fluctuations And Driven Response Of Insect Swarms, Rui Ni, James G. Puckett, Eric R. Dufresne, Nicholas T. Ouellette

Physics and Astronomy Faculty Publications

Animals of all sizes form groups, as acting together can convey advantages over acting alone; thus, collective animal behavior has been identified as a promising template for designing engineered systems. However, models and observations have focused predominantly on characterizing the overall group morphology, and often focus on highly ordered groups such as bird flocks. We instead study a disorganized aggregation (an insect mating swarm), and compare its natural fluctuations with the group-level response to an external stimulus. We quantify the swarm’s frequency-dependent linear response and its spectrum of intrinsic fluctuations, and show that the ratio of these two quantities has …

Evaluating Sub-Lethal Effects Of Orchard-Applied Pyrethroids Using Video-Tracking Software To Quantify Honey Bee Behaviors, Erin M. Ingram, Julie Augustin, Marion D. Ellis, Blair Siegfried

Department of Entomology: Faculty Publications

Managed honey bee, Apis mellifera L., colonies are contracted to pollinate fruit and nut orchards improving crop quality and yield. Colonies placed in orchards are potentially exposed to pyrethroid insecticides used for broad-spectrum pest control. Pyrethroids have been reported to pose minimal risk to bees due to their low application rates in the field and putative repellent properties. This repellency is believed to alter foraging behavior with the benefit of preventing bees from encountering a lethal dose in the field. However, sub-lethal exposure to pyrethroids may adversely impact bee behavior potentially resulting in social dysfunction or disruption of foraging.

This …

Climate-Adapted Companion Cropping Increases Agricultural Productivity In East Africa, Charles A.O. Midega, Toby J.A. Bruce, John A. Pickett, Jimmy O. Pittchar, Alice Murage, Zeyaur R. Khan

All Peer-Reviewed Publications

Production of cereals, the main staple and cash crops for millions of farmers in sub-Saharan Africa (SSA) is severely constrained by parasitic striga weed Striga hermonthica, stemborers and poor soil fertility. A companion cropping system known as 'push-pull' overcomes these constraints while providing additional soil fertility and forage grass benefits to smallholder farmers. To ensure the technology's long-term sustainability in view of the current and further potential aridification as a consequence of climate change, drought-tolerant crops, Brachiaria cv mulato (border crop) and greenleaf desmodium (intercrop), have been identified and incorporated into a 'climate-adapted push-pull'. The aims of the current study …

Biochemical, Physiological, And Anatomical Insights Into Aphid-Bioenergy Switchgrass Interactions, Travis J. Prochaska

Department of Entomology: Dissertations, Theses, and Student Research

Switchgrass, Panicum virgatum L., a perennial, warm-season grass native to North America, is a candidate for development as a bioenergy crop. Previously, warm-season grasses were considered to be relatively pest free in their native habitats. However, recent studies using the hemipteran family Aphididae have shown phloem-feeding insects can lead to significant injury in switchgrass. The objectives of this research were to: 1) gain physiological, biochemical, and anatomical insights into insect-bioenergy switchgrass interactions to determine potential insect resistance mechanisms among susceptible and resistant switchgrass genotypes; and 2) to generate and evaluate diverse segregating populations of switchgrass, both resistant and susceptible, to …

Adoption Of Modern Beekeeping And Its Impact On Honey Production In The Former Mwingi District Of Kenya: Assessment Using Theory-Based Impact Evaluation Approach, Hippolyte D. Affognon, W. S. Kingori, A. I. Omondi, M. G. Diiro, B. W. Muriithi, S. Makau, S. K. Raina

All Peer-Reviewed Publications

This study used the theory-based impact evaluation approach to understand as to how promotion of beekeeping by the Commercial Insects Programme (CIP) of the International Centre of Insect Physiology and Ecology (icipe) has had an impact on honey production in the former Mwingi District of Kenya (now merged into Kitui County). We examined the adoption of modern hives promoted by icipe and applied data envelopment analysis (DEA) to assess the technical efficiency (TE) of participants in CIP. CIP participants had an average TE of 0.56, which was significantly higher than that of non-participants in the former Kitui District (average TE …

Predicting The Impact Of Temperature Change On The Future Distribution Of Maize Stem Borers And Their Natural Enemies Along East African Mountain Gradients Using Phenology Models, Sizah Mwalusepo, Henri E.Z. Tonnang, Estomih S. Massawe, Gerphas O. Okuku, Nancy Khadioli, Tino Johansson, Paul André Calatayud, Bruno Pierre Le Ru

All Peer-Reviewed Publications

Lepidopteran stem borers are among the most important pests of maize in East Africa. The objective of the present study was to predict the impact of temperature change on the distribution and abundance of the crambid Chilo partellus, the noctuid Busseola fusca, and their larval parasitoids Cotesia flavipes and Cotesia sesamiae at local scale along Kilimanjaro and Taita Hills gradients in Tanzania and Kenya, respectively. Temperature-dependent phenology models of pests and parasitoids were used in a geographic information system for mapping. The three risk indices namely establishment, generation, and activity indices were computed using current temperature data record from local …

Time-Frequency Analysis Reveals Pairwise Interactions In Insect Swarms, James G. Puckett, Rui Ni, Nicholas T. Ouellette

Physics and Astronomy Faculty Publications

The macroscopic emergent behavior of social animal groups is a classic example of dynamical self-organization, and is thought to arise from the local interactions between individuals. Determining these interactions from empirical data sets of real animal groups, however, is challenging. Using multicamera imaging and tracking, we studied the motion of individual flying midges in laboratory mating swarms. By performing a time-frequency analysis of the midge trajectories, we show that the midge behavior can be segmented into two distinct modes: one that is independent and composed of low-frequency maneuvers, and one that consists of higher-frequency nearly harmonic oscillations conducted in synchrony …

Speed And Resolution In The Age Of Technological Reproducibility, Shawn Taylor

Theses and Dissertations

The rate of acceleration of the biologic and synthetic world has for a while now, been in the process of exponentially speeding up, maxing out servers and landfills, merging with each other, destroying each other. The last prehistoric relics on Earth are absorbing the same oxygen, carbon dioxide and electronic waves in our biosphere as us. A degraded .jpeg enlarged to full screen on a Samsung 4K UHD HU8550 Series Smart TV - 85” Class (84.5” diag.). Within this composite ecology, the ancient limestone of the grand canyon competes with the iMax movie of itself, the production of Mac pros, …

The Impacts Of Water Availability On Macroinvertebrate, Ector Matias Martell

Open Access Theses & Dissertations

In many regions around the world climate is changing and biotic communities are changing with it. In areas like the southwestern U.S., drought is one of the major sources of disturbance. Although droughts are an integral part of the natural hydrological cycles, they can have devastating effects on freshwater ecosystems when disrupted by extended periods of drought, resulting in changes to the structure and function of biotic communities. In this study, we surveyed four streams in the Sacramento Mountains of New Mexico from 2010 to 2014: Agua Chiquita, Rio Peñasco, Wills Creek, and the Sacramento River; These years encompassed an …

Effect Of Temperature On The Phenology Of Chilo Partellus (Swinhoe) (Lepidoptera, Crambidae); Simulation And Visualization Of The Potential Future Distribution Of C. Partellus In Africa Under Warmer Temperatures Through The Development Of Life-Table Parameters, N. Khadioli, Z. E.H. Tonnang, E. Muchugu, G. Ong'amo, T. Achia, I. Kipchirchir, J. Kroschel, B. Le Ru

All Peer-Reviewed Publications

Maize (Zea mays) is a major staple food in Africa. However, maize production is severely reduced by damage caused by feeding lepidopteran pests. In East and Southern Africa, Chilo partellus is one of the most damaging cereal stem borers mainly found in the warmer lowland areas. In this study, it was hypothesized that the future distribution and abundance of C. partellus may be affected greatly by the current global warming. The temperature-dependent population growth potential of C. partellus was studied on artificial diet under laboratory conditions at six constant temperatures (15, 18, 20, 25, 28, 30, 32 and 35 °C), …

Microclimate Modification And Insect Pest Exclusion Using Agronet Improve Pod Yield And Quality Of French Bean, Elisha Otieno Gogo, Mwanarusi Saidi, Jacob Mugwa Ochieng, Thibaud Martin, Vance Baird, Mathieu Ngouajio

All Peer-Reviewed Publications

French bean [Phaseolus vulgaris (L.)] is among the leading export vegetable in Africa, mostly produced by small-scale farmers. Unfavorable environmental conditions and heavy infestations by insect pests are among the major constraints limiting production of the crop. Most French bean producers grow their crop in open fields outdoors subject to harsh environmental conditions and repeatedly spray insecticides in a bid to realize high yield. This has led to rejection of some of the produce at the export market as a result of stringent limits on maximum residue levels. Two trials were conducted at the Horticulture Research and Teaching Field, Egerton …

Predicting The Impact Of Climate Change On Regional And Seasonal Abundance Of The Mealybug Phenacoccus Solenopsis Tinsley (Hemiptera: Pseudococcidae) Using Temperature-Driven Phenology Model Linked To Gis, Babasaheb B. Fand, Henri E.Z. Tonnang, Mahesh Kumar, Santanu K. Bal, Naveen P. Singh, D. V.K.N. Rao, Ankush L. Kamble, Dhananjay D. Nangare, Paramjit S. Minhas

All Peer-Reviewed Publications

The mealybug Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) is a highly invasive and polyphagous pest of global incidence. The fundamental hypothesis of the present study was that the temperature variations due to global climate change may affect seriously the future distribution and abundance of P. solenopsis, which might further aggravate the crop yield losses. We employed a temperature-based phenology model of P. solenopsis in a geographic information system for mapping population growth potentials of P. solenopsis. The three risk indices viz., establishment risk index, generation index and activity index were computed using interpolated temperature data from worldclim database for current (2000) …

Vector Competence Of Aedes Aegypti Populations From Kilifi And Nairobi For Dengue 2 Virus And The Influence Of Temperature, Edith Chepkorir, Joel Lutomiah, James Mutisya, Francis Mulwa, Konongoi Limbaso, Benedict Orindi, Zipporah Ng'ang'a, Rosemary Sang

All Peer-Reviewed Publications

Background: Susceptibility of Ae. aegypti mosquito to dengue virus (DENV) varies geographically and can be influenced by climatic factors such as temperature, which affect the incidence, seasonality and distribution of vector-borne diseases. The first outbreak of dengue fever (DF) in Kenya occured in 1982 in the coastal towns of Malindi and Kilifi. Unlike Nairobi where no active dengue transmission has been reported, DF is currently re-emerging at the Coast causing major outbreaks. This study investigated the vector competence of Ae. aegypti populations from two urban areas, Kilifi (Coast of Kenya) and Nairobi (Central Kenya), for DEN-2 virus and the influence …