Entomology Commons^™

Detection Of Trypanosome Infections In The Saliva Of Tsetse Flies And Buffy-Coat Samples From Antigenaemic But Aparasitaemic Cattle, P. A.O. Majiwa, R. Thatthi, S. K. Moloo, J. H.P. Nyeko

All Peer-Reviewed Publications

Relatively simple protocols employing non-radioactive DNA probes have been used for the detection of African trypano-somes in the blood of mammalian hosts and the saliva of live tsetse flies. In combination with the polymerase chain reaction (PCR), the protocols revealed trypanosomes in buffy-coat samples from antigenaemic but aparasitaemic cattle and in the saliva of live, infected tsetse flies. Furthermore, the protocols were used to demonstrate concurrent natural infections of single tsetse flies with different species of African trypanosomes. © 1994, Cambridge University Press. All rights reserved.

Isolation Of Trypanosoma Spp. From Wild Tsetse Flies Through Procyclic Expansion In Glossina Morsitans Centralis, Steve Mihok, Eli N. Munyoki, Freddie Masaninga, Paul N. Ndegwa, Reardon O. Olubayo

All Peer-Reviewed Publications

Procyclic trypanosomes from wild tsetse flies were membrane-fed to Glossina morsitans centralis in order to develop an optimal technique for propagating field isolates. A 70% success rate was achieved in isolating Trypanosoma simiae and a variety of genotypes of T. congolense originating from G. pallidipes, G. brevipalpis and G. swynnertoni. Parasites matured into forms infective for mammals, and could be maintained by passage of gut forms to new groups of flies. In experiments with laboratory stocks, we also passaged immature gut infections of T. congolense and T. brucei from various tsetse species to G. m. centralis. The optimal technique was …

Trypanosoma Simiae In The White Rhinoceros (Ceratotherium Simum) And The Dromedary Camel (Camelus Dromedarius), Steve Mihok, Erich Zweygarth, Eli N. Munyoki, John Wambua, Richard Kock

All Peer-Reviewed Publications

Trypanosoma simiae was identified as the cause of a disease outbreak in dromedary camels (Camelus dromedarius) introduced to Tsavo East National Park, confirming the susceptibility of camels to this pathogen. T. simiae was also isolated from a new host, the white rhinoceros (Ceratotherium simum) through xenodiagnosis with a susceptible tsetse species (Glossina morsitans centralis). A white rhinoceros showed some evidence of anaemia and lymphopaenia when harbouring T. simiae, but did not suffer any long-term health effects. © 1994.

Experimental Immunity Against Trypanosomiasis In Rabbits, Curtis Powell

All Peer-Reviewed Publications

Rabbits were immunized with specially prepared subcellular fractions of a cloned Trypanosoma brucei brucei stock 427. One fraction was found to be highly immunoprophylactic in rabbits exposed to the vector fly genus Glossina m. morsitans (tsetse flies) infected with EATRO Trypanosoma brucei brucei 1969. The most immunoprotective fraction IPFA from T. b. brucei 427 gave 75% protection to animals challenged by EATRO T. b. brucei 1969. IPFA also showed the highest IgG antibody titre against the heterologous and homologous strains. In in vitro studies, the sera from these immunized rabbits killed different strains of parasites, T. b. brucei strains 1969 …

The Influence Of Host Blood On Infection Rates In Glossina Morsitans Sspp. Infected With Trypanosoma Congolense, T. Brucei And T. Simiae, S. Mihok, R. O. Olubayo, N. Darji, E. Zweygarth

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Trypanosoma congolense, T. brucei and T. simiae isolated from wild-caught Glossina pallidipes were fed to laboratory-reared G. morsitans centralis and G. m. morsitans to determine the effect of host blood at the time of the infective feed on infection rates. Bloodstream forms of trypanosomes were membrane-fed to flies either neat, or mixed with blood from cows, goats, pigs, buffalo, eland, waterbuck and oryx. The use of different bloods for the infective feed resulted in differences in infection rates that were repeatable for both tsetse subspecies and most parasite stocks. Goat, and to a lesser extent, pig blood facilitated infection, producing …

Inhibitory Effect Of Trypanosoma Brucei Brucei On Glossina Morsitans Midgut Trypsin In Vitro, M. O. Imbuga, E. O. Osir, V. L. Labongo

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The ability of Trypanosoma brucei brucei to inhibit trypsin or trypsin-like enzymes in crude midgut homogenates of Glossina morsitans morsitans was studied in vitro. The isolated parasites caused a concentration-dependent decrease in midgut trypsin activity. Furthermore, trypanosomes lysed by repeated freeze-thawing had a similar effect on trypsin activity. In both cases, the inhibition by either intact or lysed parasites was partial as revealed by Dixon plots. Similarly, trypanosome membrane proteins stoichiometrically inhibited trypsin activity, suggesting that the enzyme interacts specifically with a moiety on the parasite surface. The Km and Ki values obtained in this case were 35 μm and …

Trypanosome Infection Rates In Tsetse Flies (Diptera: Glossinidae) And Cattle During Tsetse Control Operations In The Kagera River Region Of Rwanda, Steve Mihok, Leonard H. Otieno, Christopher S. Tarimo

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Trypanosome infections were monitored in three species of tsetse fly (Glossina pallidipes Austen, G. morsitans centralis Machado, and G. brevipalpis News-tead) at four locations in the Kagera River region of Rwanda from May 1989 to September 1990. Two of the four areas (Mpanga Ranch and Bukora Ranch) were subjected to tsetse fly suppression operations with odour-baited traps. Proboscis infections of the Trypanosoma congolense and T. vivax types accounted for roughly equal numbers of the 207 mature infections detected (3.8%). Variation in infection rates was area-specific rather than tsetse species-specific. Order of magnitude differences in tsetse fly densities among areas were …

Studies On Tsetse Midgut Factors That Induce Differentiation Of Bloodstream Trypanosoma Brucei Brucei In Vitro, M. O. Imbuga, E. O. Osir, V. L. Labongo, N. Darji, L. H. Otieno

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An in vitro system for studying the transformation of bloodstream forms of Trypanosoma brucei brucei into procylic (midgut) forms is described. In this system, transformation of the parasites was stimulated by Glossina morsitans morsitans midgut homogenates at 27° C but not at 4° C. The transformation-stimulating capacity was irreversibly destroyed by heating the midgut homogenates at 60° C for 1 h. A correlation was established between the transformation activity of the midgut homogenates and trypsin activity. The protease inhibitors (soybean trypsin inhibitor and N-p-tosyl-l-lysine-chloromethyl-ketone) inhibited trypsin activity and completely blocked the transformation of the parasites. Furthermore, the midgut homogenates could …

Influence Of D(+)-Glucosamine On Infection Rates And Parasite Loads In Tsetse Flies (Glossina Spp.) Infected With Trypanosoma Brucei, Steve Mihok, Leonard H. Otieno, Nafisa Darji, David Munyinyi

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Teneral Glossina morsitans centralis, G. m. morsitans and G. pallidipes were infected with three different clones of Trypanosoma brucei in blood containing d(+)-glucosamine, an inhibitor of tsetse midgut lectin. On average, 5 days of d(+)-glucosamine treatment tripled infection rates, without affecting the proportion of infections that matured. Total infection rates were equal in males and females, but twice as many infections matured in males. Counts of parasites in the guts and salivary glands of 277 flies revealed order of magnitude differences among flies, with females consistently having 2-3-times as many parasites as males. Parasite numbers varied in a sex-specific manner …

Infection Rates In Glossina Morsitans Morsitans Fed On Waterbuck And Boran Cattle Infected With Trypanosoma Congolense, Steve Mihok, Reardon O. Olubayo, David F. Wesonga

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Teneral Glossina morsitans morsitans were fed on waterbuck (Kobus defassa) and Boran cattle (Bos indicus) infected experimentally with Trypanosoma congolense clone IL2895. Infection rates in tsetse varied from 9 to 31% when fed on cattle, and from 2 to 59% when fed on waterbuck. In waterbuck, infections were often detected through the development of parasites in tsetse at times when parasitaemia could not be detected through microscopic examination of blood. Male and female, and 1- and 2-day-old flies were equally susceptible to infection on both hosts. Infection in tsetse was associated with a 14% absolute reduction in survival during the …

Pathogenicity Of Tsetse-Transmitted Trypanosoma Congolense For Waterbuck (Kobus Defassa) And Boran Cattle (Bos Indicus), R. O. Olubayo, S. Mihok, D. F. Wesonga, E. R.M. Mbwabi

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Five waterbuck (Kobus defassa) and four Boran cattle (Bos indicus) were infected with Trypanosoma congolense IL2895 using Glossina morsitans morsitans. At the same time, two waterbuck and two cattle were inoculated intravenously with bloodstream forms. With both methods of challenge, cattle had short prepatent periods followed by a continuous high parasitaemia. All cattle became severely anaemic and had to be treated with trypanocidal drugs to prevent death. In contrast, tsetse and intravenous challenge of waterbuck resulted in a long prepatent period, followed by brief, intermittent levels of low parasitaemia, and eventual selfcure. Waterbuck did not become anaemic, even during short …

Sheep Lice : Prospects For Eradication, F C. Wilkinson

Journal of the Department of Agriculture, Western Australia, Series 4

Sheep biting lice have been controlled over the past 86 years in Western australia by a combination of routine insecticidal treatment after shearing and action by the department of Agriculture officers under legislation.

In July 1987 the sheep industry and the State Government will implement a plan to eradicate lice from sheep flocks in the agricultural areas and eventually in the pastoral areas. To achieve this the co-operation of many sectors of the industry will be required. Assuming co-operation is forthcoming, it is possible that by 1996 wool from agricultural areas will be free of lice and insecticidal treatment form …

A Spray Programme For Table Grape Production In Western Australia, I. J. Cameron, P. Wood, A. Sproul

Viticulture published reports

This bulletin details the main sprays that can be used in table grape vineyards in Western Australia. Growers are advised to refer to the manufacturer's label for detailed recommendations since rates vary according to formulation. New chemicals are continually being marketed and any omissions are regretted and no preferences for any material or supplier is implied.

Bulletin No 4101 - Flystrike - A Manual For Its Prevention And Control, N Monzu Dr

Bulletins 4000 -

The sheep blowfly is still regarded as the Australian sheep industry's most­damaging pest. Estimates of the cost to the industry, through production losses and prevention and control measures, exceed $ I 00 million in a year. This bulletin comprises the most relevant information available on:

• Blowfly types and life cycles

• Conditions predisposing sheep to flystrike

• The mechanism of flystrike

• Methods of preventing and controlling strike.

• Current research - including the development of a forecasting system for body strike.

'Dermo' Fleece Rot And Body Strike On Sheep, S G. Gherardi, S. S. Sutherland, N. Monzu

Journal of the Department of Agriculture, Western Australia, Series 4

Blowfly strike is one of the major problems confronting the sheep industry in Austrsalia, with an estimated totsalcost of control eceeding $100 million each year.

Sheep are susceptible to five types of strike: body, breach, poll, pizzle and wound. Body strike, is of greater economic importance because its sporadic occurence from year to year makes it difficult to predict and cintrol. Outbreaks of body strike after rain can affect large numbers of sheep and inflict severe production losses.

In eastern Australia fleece rot is generally considered to be the main predisposing factor to blowfly strike, but in Western Australia evidence …

Allergies Related To Mosquitoes, Repellents, And Insecticides, James T.C. Li, Charles E. Reed

Journal of the Minnesota Academy of Science

Man's skin response to the mosquito bite exhibits great individual variability. The everyday immediate reaction consists o f a red, or erythematous wheal that lasts only one or two hours. Twenty to 24 hours after the mosquito bite, a delayed reaction of erythema, swelling, and itching may also occur. An individual may exhibit an immediate reaction, delayed reaction, both reactions, or neither reaction. Studies suggest that sensitization to mosquito saliva may be responsible for the inflammatory response. This hypothesis is supported by histologic studies which demonstrate striking infiltration of inflammatory cells at the site of mosquito bites. Severe local reactions …

Mosquitoes As Vectors Of Disease In Minnesota, John W. Washburn

Journal of the Minnesota Academy of Science

Diseases due to mosquito-borne viruses occur every summer in Minnesota. The incidence of Western encephalitis and LaCrosse encephalitis is usually low, but outbreaks of Western encephalitis have occurred in the past. Evidence of Jamestown Canyon virus activity has been found in Minnesota. This virus may represent a newly-recognized cause of central nervous system disease and encephalitis. The epidemiology of the mosquito-borne encephalitis viruses found in Minnesota and the methods of disease surveillance and control are discussed.

The Bush Tick, R B. Besier

Journal of the Department of Agriculture, Western Australia, Series 4

A newly discovered livestock parasite has focused attention on farms in the Walpole district in the south-west of Western Australia. Ticks submitted to the Department of Agriculture in December 983 were identified as the bush tick, Haemaphysalis longicornis. An intensive campaign to locate areas of infestation and to limit spread was started in January 1984.

The bush tick is a pest of livestock in some areas of New South Wales and Queensland. It is important in a narrow coastal strip from Sydney to Brisbane where total summer rainfall can exceed 1000 mm. In this area heavy tick infestation causes …

Minnesota's Response: The Mosquito Research Program, John Washburn, Nancy Read

Journal of the Minnesota Academy of Science

The Minnesota Mosquito Research Program (MMRP) was established in September 1984 in response to a request by Governor Perpich. The Minnesota Department of Health (MDH), as the lead agency, brought together a Working Group of distinguished North American experts to discuss the problems related to mosquito research and control. This Working Group- with advice from legislators and representatives from the community, environmental groups, and state and federal agencies- made recommendations for the development of a mosquito research and control program in Minnesota.

The Working Group divided its recommendations into two areas, research and administrative structure. An extensive research program in …

United States Naval Medical Research Unit Number Three, Cairo, Egypt, Medical Zoology Department Translation List, Harry Hoogstraal

United States Naval Medical Research Unit 3: Publications

List of translations of medical parasitology and medical entomology papers prepared by the United States Naval Medical Research Unit Number 3 in Cairo, Egypt. The papers were translated from numerous languages, such as Russian (most were translated from Russian), Bulgarian, Chinese, Czech, Finnish, French, German, and Japanese, into English. The publication dates of the original papers ranged from about 1915 to 1983. Harry Hoogstraal translated some or all of the papers.

A Technique For Physiologically Age-Grading Female Stable Flies, Stomoxys Calcitrans (L.), P. J. Scholl

Nebraska Agricultural Experiment Station: Historical Research Bulletins

A method of dissection followed by staining was initiated to allow physiological age- grading based on ovarian development in order to more completely describe the physiological development of adult female stable flies [Stomoxys calcitrans (L. )]. The gonotrophic developmental continuum from a non-differentiated cell in teneral females to mature eggs at the time of oviposition was first arbitrarily divided into six stages by using distinct landmarks within the developing oocyte . Then, nulliparous, uniparous, and biparous+ females were differentiated on the basis of the presence or absence of stained follicular relics in the ovariole sheaths. The combination of the …

Mulesing : Still The Best Weapon Against Fly-Strike, R A. Mills

Journal of the Department of Agriculture, Western Australia, Series 4

IN SPITE of modern chemicals and insecticides, blow-fly strike remains one of the major problems facing sheep farmers today.

Flystrike Control Methods, H E. Fels

Journal of the Department of Agriculture, Western Australia, Series 4

A short article outlining methods to control flystrike.

Control Of Insects In Cotton, D G. Shedley, D. L. Hardy, P. J. Michael

Journal of the Department of Agriculture, Western Australia, Series 4

Results of two experiments at the Ord Irrigation area in 1968 indicate that farmers may be spending more than necessary to obtain economic control of cotton pests in some seasons.

One experiment clearly demonstrated the relationship between low insect numbers and improved cotton yields—the second indicated that a moderate but well timed spray schedule, especially during the early part of the season, can result in more economic insect control than an intensive spray schedule.

A Brief History Of The Namru-3 Medical Zoology Program, Harry Hoogstraal

United States Naval Medical Research Unit 3: Publications

The NAMRU-3 Medical Zoology program began during Easter week of 1950. We had just closed the NAMRU-3 field station at Torit, in Equatoria Province of soulheastern Sudan, and were heading homeward to the United States. However, the offer to remain in Egypt for a year or two to develop a Medical Zoology department at NAMRU-3 provided an interesting challenge after working for several years in tropical forests and grasslands of Africa and other continents. We shall briefly describe subsequent events under four headings: (1) Medical Zoology in Egypt, (2) Ticks and Tickborne Diseases of the World, (3) Kala Azar in …

Arthropods Of Medical Importance In Asia (And The European Ussr), Part Ii: Data On Arthropods Other Than Mosquitoes, Bernard V. Travis, Renato M. Labadan

Harold W. Manter Laboratory of Parasitology: Library Materials

Includes information on black flies, sand flies, midges, horse flies, biting flies, non-biting flies, fleas, bugs, urticating and vesicating arthropods, ticks, mites, and miscellaneous arthropods of medical importance from Asia and the European USSR.

The Fecal Shedding Of Listeria Monocytogenes By Conventional And "Germ Free" Mice, Margaret Joyce Ramsay

Loma Linda University Electronic Theses, Dissertations & Projects

A series of experiments involving Listeria monocytogenes was undertaken to study first the fecal shedding of Listeria by conventional mice challenged intraperitoneally with Listeria and second the fecal shedding and ability to survive of germ free mice with similar challenge. In addition, mites infesting Listeria infected mice were cultured for their possible contamination by Listeria.

Conventional mice were challenged intraperitoneally with a single near LD₅₀ dose of Listeria. Fecal Listeria were detected by collecting specimens from each mouse daily for the first 4 days and every second day thereafter. The feces was held at 7°C for 14 …

Did This Cause This? [Sarcoptic Mange Of Pigs], F C. Wilkinson

Journal of the Department of Agriculture, Western Australia, Series 4

The mite on the left was detected by using a microscope with a magnification of 100 times to examine a skin scraping taken from the above pig.

This confirmed the suspicion that sarcoptic mange mites were the cause of the skin lesions.

The Animal Quarantine Service, R K. Kent

Journal of the Department of Agriculture, Western Australia, Series 4

Exotic diseases are a constant threat to Australia's animal industry and there are many reasons for the uncompromising ban on the import of certain animals from overseas.

Such diseases as foot and mouth and blue tongue would cost Australia a tremendous amount of money if they became established here.

Just how Australia protects itself against the introduction of exotic diseases is described by Veterinary Surgeon, R. K. Kent, M.R.C.V.S., in this article .

Control Of Lice On Pigs, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

LICE are responsible for serious losses in the pig industry, as apart from the reduced growth rates and loss of condition resulting from the constant irritation, the lice are carriers of disease.