Entomology Commons^™

Transferability Of Tsetse Habitat Models Between Different Regions In Kenya And Rwanda, Stella Gachoki, Anton Vrieling, Thomas A. Groen, Andrew Skidmore, Daniel Masiga

All Peer-Reviewed Publications

Accurate and reliable information on the distribution of tsetse habitats is crucial for the effective management of African Trypanosomiasis in sub-Saharan Africa. However, conducting large-scale surveillance of tsetse flies to develop distribution maps is impractical due to vast areas infested and limited resources available. To address this challenge, we evaluated the applicability of tsetse habitat models developed in the intensively sampled Shimba Hills National Reserve in Kenya for both the wet and the dry season, to two other regions in Kenya (Ruma National Park and Nguruman Conservancy) and one region in Rwanda (Akagera National Park). The models utilized satellite-based estimates …

Impacts On Malaria Transmission Of Altered Biogenic Amine Levels In {\It Anopheles} Mosquitoes: Insights From A Mathematical Model, Michael Robert

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.

Phylogenomics Reveals The History Of Host Use In Mosquitoes, John Soghigian, Charles Sither, Silvia Andrade Justi, Gen Morinaga, Brian K. Cassel, Christopher J. Vitek, Todd Livdahl, Siyang Xia, Andrea Gloria-Soria, Jeffrey R. Powell

School of Integrative Biological & Chemical Sciences Faculty Publications

Mosquitoes have profoundly affected human history and continue to threaten human health through the transmission of a diverse array of pathogens. The phylogeny of mosquitoes has remained poorly characterized due to difficulty in taxonomic sampling and limited availability of genomic data beyond the most important vector species. Here, we used phylogenomic analysis of 709 single copy ortholog groups from 256 mosquito species to produce a strongly supported phylogeny that resolves the position of the major disease vector species and the major mosquito lineages. Our analyses support an origin of mosquitoes in the early Triassic (217 MYA [highest posterior density region: …

Histamine Excretion In Common Indoor And Hematophagous Arthropods, Simona Principato, Alvaro Romero, Chow-Yang Lee, Kathleen Campbell, Dong-Hwan Choe, Coby Schal, Zachary C. Devries

UK CARES Faculty Publications

Histamine is a biogenic amine that regulates multiple physiological functions in diverse organisms, specifically playing a central role in the mammalian immune response. The common bed bug, Cimex lectularius L. (Hemiptera: Cimicidae), excretes histamine in large amounts in its feces as a component of its aggregation pheromone, which contaminates homes. The potential health risks associated with the presence of indoor histamine are unclear, but to predict future exposure risks, it is critical that we understand if other arthropods excrete histamine in any discernible phylogenetic pattern. In the present study, we evaluated histamine excretion by various arthropods; specifically those commonly found …

Effect Of Use Of Tsetse Repellant Collar Technology On The Farm Performance And Household Welfare Of Small-Scale Livestock Farmers In Kenya, Beatrice W. Muriithi, Kassie Menale, Gracious M. Diiro, Michael N. Okal, Daniel K. Masiga

All Peer-Reviewed Publications

Tsetse-transmitted Animal African Trypanosomosis (AAT) is a disease of economic importance to livestock development in Sub-Saharan Africa. The disease is widely managed using trypanocides that are expensive for smallholders and associated with health risks due to drug residues in animal products and drug resistance. Developed recently by the International Centre of Insect Physiology and Ecology (icipe) and partners, the novel Tsetse repellent collar technology (TRCT) provides an effective alternative control measure that targets the tsetse fly vector of AAT. This study assessed the impact of TRCT on household welfare and food security by using ex-post non-experimental data obtained from 632 …

Effect Of Temperature On The Microbiome Of A Laboratory Reared Colony Of Haemaphysalis Longicornis Ticks, Brianna Mitchell

Poster Presentations

Honors research poster.

Haemaphysalis longicornis is a species of tick native to eastern Asia, including eastern China, Japan, eastern Russia, and Korea. To date, it has invaded and has now established its existence in Australia, New Zealand, and several of the Pacific Islands. This species of tick has recently been introduced to the United States, having a confirmed sighting in November 2017 on a sheep farm in New Jersey and since establishing populations in 18 states along the east coast and Appalachia. Based on the existing locations of H. longicornis in its native regions, as well as in the United …

Prevalence Of Ehrlichia And Rickettsia Within Ticks In Arkansas State Parks, Haley Stanton

Animal Science Undergraduate Honors Theses

Within the past few decades, the number of diagnoses of tick-borne diseases – such as spotted fever rickettsiosis and ehrlichiosis – has steadily increased throughout the United States, with Arkansas having one of the highest rates in the country. These diseases pose a risk to both humans and animals, as both can acquire these infections from ticks. Due to the increasing geographic range of ticks, rise in cases, and likely underdiagnosis of these diseases, this study aimed to identify the infection rate of ticks themselves in multiple state parks in Arkansas. Four Arkansas state parks were each visited once a …

Effect Of Temperature On The Microbiome Of A Laboratory-Reared Colony Of Haemaphysalis Longicornis Ticks, Brianna L. Mitchell

Honors Theses

Haemaphysalis longicornis ticks are invasive to the United States with potential to transmit several tick-borne pathogens that are native to the United States. Based on existing locations of H. longicornis in its native regions in Asia, as well as its invasive populations that are established in the United States, several geographic range prediction models have been produced to help understand future range expansion and distribution of this invasive tick in North America. Unfortunately, these models do not all agree and there is uncertainty associated with the potential geographic range expansion of H. longicornis ticks in North America. Climate can affect …

Prevalence Of Dog Heartworm (Dirofilaria Immitis) Infection In Invasive Aedes Aegypti And Aedes Albopictus Mosquitoes In Southern California, Irene Hui Ying Lee, Donghwi Kim, Romina Lee

Pacific Undergraduate Research and Creativity Conference (PURCC)

No abstract provided.

Characterizing Movement Patterns Of Nomadic Pastoralists And Their Exposure To Rift Valley Fever In Kenya, G. Mosomtai, J. L. Kasiiti, R. M. Murithi, P. Sandström, T. Landmann, O. W. Lwande, O. A. Hassan, C. Ahlm, R. Sang, M. Evander, Z. Szantoi, G. Ottavianelli

All Peer-Reviewed Publications

The role of animal movement in spreading infectious diseases is highly recognized by various legislations and institutions such as the World Organisation for Animal Health and the International Animal Health Code. The increased interactions at the nexus of human-animal-ecosystem interface have seen an unprecedented introduction and reintroduction of new zoonotic diseases with high socio-economic impacts such as the COVID-19 pandemic. Rift Valley fever (RVF) is a zoonotic disease that affects both humans and animals and is transmitted by Aedes mosquitoes or through contact with the body fluids of infected animals. This study seeks to characterize movement patterns of pastoralist and …

Entomophagy: Mealworm Protein In An Inpatient Hospital Setting (Engagement And Education To Rollout Entomophagy For Improved Nutrition), Caryl Showalter

Department of Entomology: Distance Master of Science Projects

The consumption of insects (entomophagy) is a well-practiced phenomenon in many parts of the world. Western societies, including the US, are among the few places that do not participate in this practice. However, entomophagy has the potential to ease nutritional burdens, globally. While many may believe that nutritional deficits are a relic of more economically challenging times or a problem specifically linked to the low economic countries, the truth is that any area can face this problem. This was made clear in the US during the coronavirus lockdown with an estimated doubling in food insecurity associated with a lack of …

Nature Journaling With Insects: A Guide And Journal Template, Danielle Positeri

Department of Entomology: Distance Master of Science Projects

An Introduction to Nature Journaling

Nature journaling is a natural history based activity centered on journaling one’s experiences and observations based on surrounding environment and natural lifeforms encountered. A tool commonly used by those who study natural history, famous naturalists such as Charles Darwin and John Muir were known to keep nature journals. Although those who make their careers in environmental sciences or field work keep professional, very detailed journals, journaling can also be a great activity for those who simply enjoy nature. Public educators teach nature journaling to young students to encourage building a plethora of skills: mindfulness, attention …

Bugged By Bugs: A Comprehensive Guide To Pest Control, Brandon Runyon

Department of Entomology: Distance Master of Science Projects

This book aims to create a user-friendly guide tocommercial pest control practices covering German cockroaches, bed bugs, odorous house ants, pavement ants, brown recluse spiders, wolf spiders, house flies, and fruit flies. We will address these pests in the following locations: hotels, restaurants, apartments, and offices.

Surveillance For Ticks And Tick-Borne Pathogens In Kentucky, Anna Rosalee Pasternak

Theses and Dissertations--Entomology

Tick-borne diseases are an emerging threat to human and animal health. In Kentucky, tick-borne disease surveillance has identified rising incidences of spotted fever rickettsiosis, ehrlichiosis, and Lyme disease. Since these diseases occur through the bites of infected ticks, effective prevention efforts are reliant upon knowing where the risk of exposure to tick bites exists. Historical data on tick distribution in Kentucky is variable, with very little reported on a statewide scale, leaving vector control workers, public health personnel, physicians, veterinarians, and others to rely on outdated, intermittent, or out-of-state information. In my dissertation, I surveyed ticks and select tick-borne pathogens …

Transcriptomic Profiling Of Trypanosoma Congolense Mouthpart Parasites From Naturally Infected Flies, Sara Silva Pereira, Kawira Mathenge, Daniel Masiga, Andrew Jackson

All Peer-Reviewed Publications

Background: Animal African trypanosomiasis, or nagana, is a veterinary disease caused by African trypanosomes transmitted by tsetse flies. In Africa, Trypanosoma congolense is one of the most pathogenic and prevalent causes of nagana in livestock, resulting in high animal morbidity and mortality and extensive production losses. In the tsetse fly, parasites colonise the midgut and eventually reach the mouthparts, from where they can be transmitted as the fly feeds on vertebrate hosts such as cattle. Despite the extreme importance of mouthpart-form parasites for disease transmission, very few global expression profile studies have been conducted in these parasite forms. Methods: Here, …

Identification Of Possible Vectors For Histomoniasis In Turkeys On Commercial Farms In Arkansas And Oklahoma, Treva Dillon

Graduate Theses and Dissertations

Histomonas meleagridis, is a parasitic protozoan, commonly found in the gallinaceous species of birds and is the causative agent of Blackhead disease. The cecal worm, known as Heteraskis gallinarum, acts as a vector in the spread of H. meleagridis in gallinaceous birds. H. meleagridis develops inside the cecal worm and in its eggs which allows H. meleagridis a method to survive in the environment. Blackhead disease causes high mortality rates in both chickens and turkeys; however, turkeys are noted to have a higher mortality rate compared to chickens. Molecular diagnostic testing such as PCR has been developed by creating primers …

Motociclistas Y Accidentalidad. Una Mirada Desde La Perspectiva Cultural, Juan Manuel Andrade Navia, Miguel Ángel Mahecha Bermúdez, Dagoberto Páramo Morales, Elías Ramírez Plazas

Ciencias Administrativas, Económicas y Contables

Las principales causas de accidentes de tránsito en el 2020 estaban asociadas al comportamiento humano de los actores en la vía, siendo las principales: conducir con exceso de velocidad (40,1%); no respetar las normas de tránsito (28,9 %); conducir en estado de embriaguez (9,7 %); y la violación de las normas de tránsito por los peatones (9,2 %). La ANSV en 2016 recordó un dato preocupante: el 53 % de las personas fallecidas eran motociclistas y el 55 % de los lesionados se encuentran entre 20 y 34 años, es decir, en la edad más productiva para la economía nacional. …

Entomological Assessment Of Tsetse-Borne Trypanosome Risk In The Shimba Hills Human-Wildlife-Livestock Interface, Kenya, Faith I. Ebhodaghe, Armanda D.S. Bastos, Michael N. Okal, Daniel K. Masiga

All Peer-Reviewed Publications

Shimba Hills is a wildlife area in Kenya and a major focus of tsetse-borne trypanosomes in East Africa. In Shimba Hills, tsetse-borne trypanosomes constrain animal health and smallholder livelihoods. However, epidemiological data to guide hotspot-targeted control of infections are limited. This study assessed the dynamics of tsetse-borne trypanosome risk in Shimba Hills with the objective to describe infection hotspots for targeted control. Tsetse flies (n = 696) collected in field surveys between November 2018 and September 2019 in Shimba Hills were characterized for chronological age and phenotypic sizes and screened for trypanosome and cattle DNA. Entomological inoculation rates for trypanosome …

Origins Of High Latitude Introductions Of Aedes Aegypti To Nebraska And Utah During 2019, Andrea Gloria-Soria, Ary Faraji, Jeff Hamik, Gregory White, Shanon Amsberry, Matthew Donahue, Bryan Buss, Evlyn Pless, Luciano Veiga Cosme, Jeffrey R. Powell

Department of Educational Psychology: Faculty Publications

Aedes aegypti (L.), the yellow fever mosquito, is also an important vector of dengue and Zika viruses, and an invasive species in North America. Aedes aegypti inhabits tropical and sub-tropical areas of the world and in North America is primarily distributed throughout the southern United States states and Mexico. The northern range of Ae. aegypti is limited by cold winter months and establishment in these areas has been mostly unsuccessful. However, frequent introductions of Ae. aegypti to temperate, non-endemic areas during the warmer months can lead to seasonal activity and disease outbreaks. Two Ae. aegypti incursions were …

Observing The Distribution Of Mosquito Bites On Humans To Inform Personal Protection Measures Against Malaria And Dengue Vectors, Winifrida P. Mponzi, Johnson K. Swai, Emmanuel W. Kaindoa, Khamis Kifungo, Alvaro E. Eiras, Elis P.A. Batista, Nancy S. Matowo, Peter O. Sangoro, Marceline F. Finda, Arnold S. Mmbando, Tegemeo Gavana, Halfan S. Ngowo, Fredros O. Okumu

All Peer-Reviewed Publications

Background Understanding mosquito biting behaviours is important for designing and evaluating protection methods against nuisance biting and mosquito-borne diseases (e.g. dengue, malaria and zika). We investigated the preferred biting sites by Aedes aegypti and Anopheles arabiensis on adult volunteers in standing or sleeping positions; and estimated the theoretical protection limits affordable from protective clothing or repellent-treated footwear. Methods Adult volunteers dressed in shorts and t-shirts were exposed to infection-free laboratory-reared mosquitoes inside screened chambers from 6am to noon (for day-biting Ae. aegypti) or 6pm to midnight (night-biting An. arabiensis). Attempted bites on different body parts were recorded. Comparative observations were …

Influence Of The Type Of Pollen Diet On The Survival, Body Weight, And Immune Response In The African Honeybee, Michael N.K. Muturi, Joel L. Bargul, H. Michael G. Lattorff

All Peer-Reviewed Publications

Pollen nutrition is critical for the development and well-being of the honeybee. Previous studies have compared the effect of pollen and carbohydrate-only diet on honeybee physiology. The effect of a monofloral versus polyfloral diet on the African honeybee (Apis mellifera scutellata) is poorly understood. This knowledge is critical as diversity-rich habitats are being altered to less diverse environments through increased urbanization and intensified agricultural activities, particularly in sub-Saharan Africa. Here, we report how lowly diverse (LD) and highly diverse (HD) pollen diets influence honeybee life-history traits and physiology. To achieve this, we fed caged bees with the two pollen diets …

What Is The Prevalence Of Dirofilaria Immitis (Dog Heartworm) In Invasive Aedes Mosquitoes?, Leen Yousef, Johan Espinoza Corrales, Daniela H. Dolar, Taylor K. Trieu, Aisha Popalzai, Agamchat Dhami

Pacific Undergraduate Research and Creativity Conference (PURCC)

No abstract provided.

Tick Surveillance And Pathogen Detection In Eastern South Dakota, Holly E. Black

Honors Thesis

Tick-borne diseases threaten the health of humans, animals, and ecosystems, but surveillance efforts are lacking in some regions. Throughout 2019, 2020, and 2021, we conducted tick surveys aimed at determining the status of the black-legged tick (Ixodes scapularis), the American dog tick (Dermacentor variabilis) and the lone star tick (Amblyoma americanum) in eastern South Dakota. Each year, ticks were collected using a flagging method and identified in the lab. A subset were tested for the presence of Borrelia burgdorferi, the causative agent of Lyme disease, by polymerase chain reaction (PCR). Dermacentor variabilis was the most commonly identified tick, …

Genetic Connectivity Of Trypanosomes Between Tsetse-Infested And Tsetse-Free Areas Of Kenya, Naomi N. Kimenyi, Kelvin M. Kimenyi, Nelson O. Amugune, Merid N. Getahun

All Peer-Reviewed Publications

The prevalence rates of trypanosomes, including those that require cyclical transmission by tsetse flies, are widely distributed in Africa. Trypanosoma brucei and Trypanosoma congolense are actively maintained in regions where there are no tsetse flies although at low frequencies. Whether this could be due to an independent evolutionary origin or multiple introduction of trypanosomes due to continuous movement of livestock between tsetse-free and -infested areas is not known. Thus, the aim of the study was to carry out microsatellite genotyping to explore intra-specific genetic diversity between T. (Trypanozoon), T. congolense and Trypanosoma vivax from the two regions: tsetse infested and …

Oral Susceptibility To Ivermectin Is Over Fifty Times Greater In A Wild Population Of Anopheles Albimanus Mosquitoes From Belize Than The Stecla Laboratory Reference Strain Of This Mosquito, Staci M. Dreyer, Kelsey J. Morin, Marla Magaña, Marie Pott, Donovan Leiva, Nicole L. Achee, John P. Grieco, Jefferson A. Vaughan

Biology Faculty Publications

Background

The STECLA strain of Anopheles albimanus has been in continuous colony for many years and is the reference strain on which genomic studies for the species are based. Recently, the STECLA strain was demonstrated to be much less susceptible to ivermectin ingested in a blood meal (4-day LC50 of 1468 ng/ml) than all other Anopheles species tested to-date (LC50 values range from 7 to 56 ng/ml). The ability of An. albimanus to survive ingestion of ivermectin at concentrations far beyond that typically found in the blood of ivermectin-treated people or livestock (i.e., 30–70 ng/ml) could invalidate the use of …

Larval Habitats, Species Composition And Distribution Of Malaria Vectors In Regions With Autochthonous And Imported Malaria In Roraima State, Brazil, Nathália Coelho Vargas De Almeida, Jaime Louzada, Maycon Sebastião Alberto Santos Neves, Thiago M. Carvalho, Júlio Castro‑Alves, Teresa Fernandes Silva‑Do‑Nascimento, Ananias A. Escalante, Joseli Oliveira‑Ferreira

Harold W. Manter Laboratory of Parasitology: Library Materials

Background: Malaria control requires local action. Assessing the vector diversity and abundance provides information on the local malariogenic potential or risk of transmission. This study aimed to determine the Anopheles species composition, habitats, seasonal occurrence, and distribution in areas with autochthonous and imported malaria cases in Roraima State.

Methods: A longitudinal study was conducted from January 2017 to October 2018, sampling larvae and adult mosquitoes in three municipalities of Roraima State: Boa Vista, Pacaraima and São João da Baliza. These areas have different risks of malaria importation. Four to six mosquito larval habitats were selected for larval sampling …

Editorial: Insights In Vector Biology: 2021, Baldwyn Torto, David P. Tchouassi

All Peer-Reviewed Publications

This editorial summarizes collections on the Research Topic “Insights in Vector Biology’’. Vectors of four major tropical diseases including malaria, Chagas, dengue and West Nile were considered. The editorial discusses vectors contributing to residual malaria transmission that reside mainly outdoors and not targeted by indoor vector control interventions, notably, long lasting insecticidal nets and indoor residual spraying; the concept of paratransgenesis- a parasite transmission blocking strategy which uses genetically-modified microbes made to produce antimalarial effectors inside the vector for malaria control; assessment of insecticide resistance in malaria vectors; insecticide resistance in Chagas disease vectors; reproductive biology of the dengue vector; …

Microsporidia: A Promising Vector Control Tool For Residual Malaria Transmission, Tullu Bukhari, Roland Pevsner, Jeremy Keith Herren

All Peer-Reviewed Publications

Long-lasting insecticidal nets (LLINs) and indoor residual spraying (IRS) have resulted in a major decrease in malaria transmission. However, it has become apparent that malaria can be effectively transmitted despite high coverage of LLINs/IRS. Residual transmission can occur due to Plasmodium-carrying Anopheles mosquitoes that are insecticide resistant and have feeding and resting behavior that reduces their chance of encountering the currently deployed indoor malaria control tools. Residual malaria transmission is likely to be the most significant hurdle to achieving the goal of malaria eradication and research and development towards new tools and strategies that can control residual malaria transmission is …

Farmers’ Knowledge, Perceptions, And Practices On Animal Trypanosomosis And The Tsetse Fly Vector: A Cross-Sectional Study Around Kenya’S Arabuko-Sokoke Forest Reserve At The Livestock-Wildlife Interface, Erick K. Serem, Joel L. Bargul, Moses M. Ngari, Osman A. Abdullahi, David M. Mburu

All Peer-Reviewed Publications

Background: Animal African trypanosomosis (AAT) is a veterinary disease caused by trypanosomes transmitted cyclically by tsetse flies. AAT causes huge agricultural losses in sub-Saharan Africa. Both tsetse flies and trypanosomosis (T&T) are endemic in the study area inhabited by smallholder livestock farmers at the livestock-wildlife interface around Arabuko-Sokoke Forest Reserve (ASFR) in Kilifi County on the Kenyan coast. We assessed farmers’ knowledge, perceptions and control practices towards T&T. Methods: A cross-sectional study was conducted during November and December 2017 to collect data from 404 randomly selected cattle-rearing households using a structured questionnaire. Descriptive statistics were used to determine farmers’ knowledge, …

The Combat Project: Controlling And Progressively Minimizing The Burden Of Vector-Borne Animal Trypanosomosis In Africa, Alain Boulangé, Veerle Lejon, David Berthier, Sophie Thévenon, Geoffrey Gimonneau, Marc Desquesnes, Samuel Abah, Prudenciène Agboho, Kalinga Chilongo, Tsegaye Gebre, Assane Gueye Fall, Dramane Kaba, Stefan Magez, Daniel Masiga, Enock Matovu, Aldjibert Moukhtar, Luis Neves, Pamela A. Olet, Soumaïla Pagabeleguem, William Shereni, Brice Sorli, Moeti O. Taioe, María Teresa Tejedor Junco, Rehab Yagi, Philippe Solano, Giuliano Cecchi

All Peer-Reviewed Publications

Vector-borne diseases affecting livestock have serious impacts in Africa. Trypanosomosis is caused by parasites transmitted by tsetse flies and other blood-sucking Diptera. The animal form of the disease is a scourge for African livestock keepers, is already present in Latin America and Asia, and has the potential to spread further. A human form of the disease also exists, known as human African trypanosomosis or sleeping sickness. Controlling and progressively minimizing the burden of animal trypanosomosis (COMBAT) is a four-year research and innovation project funded by the European Commission, whose ultimate goal is to reduce the burden of animal trypanosomosis (AT) …