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Full-Text Articles in Population Biology
Comparative Phylogeography Of Central African Duikers Using Non-Invasive Sampling Methods, Stephan Ntie
Comparative Phylogeography Of Central African Duikers Using Non-Invasive Sampling Methods, Stephan Ntie
University of New Orleans Theses and Dissertations
The present study sets out to assess patterns of evolutionary diversification in central African duikers (subfamily Cephalophinae). The sampling strategy consisted of collecting geo-referenced duiker feces across 43 sites and seven countries. However, several challenges related to the use of non-invasive samples needed to be addressed prior to large scale DNA amplification. First, the best storage method for obtaining DNA from fecal samples needed to be established. Our study revealed that while silica is best for nuclear microsatellite analyses, RNAlater is the best storage medium for maximal mitochondrial amplification. Moreover, extracting DNA as early as possible always provided the …