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Full-Text Articles in Population Biology

Fungicide Sensitivity Of Sclerotinia Sclerotiorum Isolates Selected From Five Different States That Use Different Fungicide Treatments, Cristian Wulkop Gil Apr 2020

Fungicide Sensitivity Of Sclerotinia Sclerotiorum Isolates Selected From Five Different States That Use Different Fungicide Treatments, Cristian Wulkop Gil

UCARE Research Products

Sclerotinia sclerotiorum is a plant pathogenic fungus that causes a disease called white mold that can infect more than 450 plant species including soybeans, dry beans, green beans, canola, and sunflower. This pathogen is capable of up to $252M in losses every year (U.S. Canola Association, 2014). Fungicides are widely used in developed agricultural systems to control disease. However, resistance to the most effective fungicides has emerged and spread in pathogen populations and there have been multiple reports of S. sclerotiorum isolates becoming resistant to certain fungicides. Since different fields in different states use different fungicide treatments on plants and …


Bacterial Community Structure Of Contrasting Soils Underlying Bornean Rain Forests: Inferences From Microarray And Next-Generation Sequencing Methods, Sabrina E. Russo, Ryan Legge, Karrie A. Weber, Eoin L. Brodie, Katherine C. Goldfarb, Andrew K. Benson, Sylvester Tan Jan 2012

Bacterial Community Structure Of Contrasting Soils Underlying Bornean Rain Forests: Inferences From Microarray And Next-Generation Sequencing Methods, Sabrina E. Russo, Ryan Legge, Karrie A. Weber, Eoin L. Brodie, Katherine C. Goldfarb, Andrew K. Benson, Sylvester Tan

School of Biological Sciences: Faculty Publications

Soil microbial diversity is vast, and we lack even basic understanding of how this diversity is distributed ecologically. Using pyrosequencing and microarray methods, we quantified the structure of bacterial communities in two contrasting soils underlying Bornean rain forest (clay and sandy loam) that differ markedly in soil properties, aboveground tree flora, and leaf litter decomposition rates. We found significant soil-related taxonomic and phylogenetic differences between communities that, due to their proximity, are independent of climate. Bacterial communities showed distinct compositional and taxon-abundance distributions that were significantly correlated with the structure of the overlying tree community. Richness of bacteria was greater …


Phylogeographical Structure And Evolutionary History Of Two Buggy Creek Virus Lineages In The Western Great Plains Of North America, Abinash Padhi, Amy T. Moore, Mary Bomberger Brown, Jerome E. Foster, Martin Pfeffer, Kathryn P. Gaines, Valerie A. O'Brien, Stephanie A. Strickler, Allison E. Johnson, Charles R. Brown Sep 2008

Phylogeographical Structure And Evolutionary History Of Two Buggy Creek Virus Lineages In The Western Great Plains Of North America, Abinash Padhi, Amy T. Moore, Mary Bomberger Brown, Jerome E. Foster, Martin Pfeffer, Kathryn P. Gaines, Valerie A. O'Brien, Stephanie A. Strickler, Allison E. Johnson, Charles R. Brown

School of Natural Resources: Faculty Publications

Buggy Creek virus (BCRV) is an unusual arbovirus within the western equine encephalitis complex of alphaviruses. Associated with cimicid swallow bugs (Oeciacus vicarius) as its vector and the cliff swallow (Petrochelidon pyrrhonota) and house sparrow (Passer domesticus) as its amplifying hosts, this virus is found primarily in the western Great Plains of North America at spatially discrete swallow nesting colonies. For 342 isolates collected in Oklahoma, Nebraska, Colorado, and North Dakota, from 1974 to 2007, we sequenced a 2076 bp region of the 26S subgenomic RNA structural glycoprotein coding region, and analyzed phylogenetic relationships, …


Experimental Inoculation Of House Sparrows (Passer Domesticus) With Buggy Creek Virus, Kathryn P. Huyvaert, Amy T. Moore, Nicholas A. Panella, Eric A. Edwards, Mary Bomberger Brown, Nicholas Komar, Charles R. Brown Jan 2008

Experimental Inoculation Of House Sparrows (Passer Domesticus) With Buggy Creek Virus, Kathryn P. Huyvaert, Amy T. Moore, Nicholas A. Panella, Eric A. Edwards, Mary Bomberger Brown, Nicholas Komar, Charles R. Brown

School of Natural Resources: Faculty Publications

We performed experimental inoculations of house sparrows (Passer domesticus) with Buggy Creek virus (BCRV), a poorly known alphavirus (Togaviridae) vectored primarily by the swallow bug (Hemiptera: Cimicidae: Oeciacus vicarius) that is an ectoparasite of the cliff swallow (Petrochelidon pyrrhonota) and house sparrow. Viremias were detected by plaque assay in two of six birds on days 1–3 postinoculation; viremia was highest on day 2. Viral RNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) in blood of six of 12 birds ranging from day 1 to day 15 postinoculation. Infectious BCRV was detected in …


Bird Movement Predicts Buggy Creek Virus Infection In Insect Vectors, Charles R. Brown, Mary Bomberger Brown, Amy T. Moore, Nicholas Komar Jan 2007

Bird Movement Predicts Buggy Creek Virus Infection In Insect Vectors, Charles R. Brown, Mary Bomberger Brown, Amy T. Moore, Nicholas Komar

School of Natural Resources: Faculty Publications

Predicting the spatial foci of zoonotic diseases is a major challenge for epidemiologists and disease ecologists. Migratory birds are often thought to be responsible for introducing some aviozoonotic pathogens such as West Nile and avian influenza viruses to a local area, but most information on how bird movement correlates with virus prevalence is anecdotal or indirect. We report that the prevalence of Buggy Creek virus (BCRV) infection in cimicid swallow bugs (Oeciacus vicarius), the principal invertebrate vector for this virus, was directly associated with the likelihood of movement by cliff swallows (Petrochelidon pyrrhonota), an amplifying host …


Ecological Correlates Of Buggy Creek Virus Infection In Oeciacus Vicarius, Southwestern Nebraska, 2004, Amy T. Moore, Eric A. Edwards, Mary Bomberger Brown, Nicholas Komar, Charles R. Brown Jan 2007

Ecological Correlates Of Buggy Creek Virus Infection In Oeciacus Vicarius, Southwestern Nebraska, 2004, Amy T. Moore, Eric A. Edwards, Mary Bomberger Brown, Nicholas Komar, Charles R. Brown

School of Natural Resources: Faculty Publications

Buggy Creek virus (family Togaviridae, genus Alphavirus, BCRV) is an alphavirus within the western equine encephalitis virus complex whose primary vector is the swallow bug, Oeciacus vicarius Horvath (Hemiptera: Cimicidae), an ectoparasite of the colonially nesting cliff swallow, Petrochelidon pyrrhonota, that is also a frequent host for the virus.We investigated ecological correlates of BCRV infection in 100-bug pools at 14 different swallow colony sites in southwestern Nebraska from summer 2004, by using plaque assay on Vero cells to identify cytopathic virus and reverse transcription-polymerase chain reaction to identify noncytopathic viral RNA. We found 26.7% of swallow bug pools …