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Full-Text Articles in Ecology and Evolutionary Biology

Fine-Scale Genetic Structure In Rhizosphere Microbial Communities Associated With Chamaecrista Fasciculata (Fabaceae), Mahboubeh Hosseinalizadeh Nobarinezhad, Lisa E. Wallace Jan 2023

Fine-Scale Genetic Structure In Rhizosphere Microbial Communities Associated With Chamaecrista Fasciculata (Fabaceae), Mahboubeh Hosseinalizadeh Nobarinezhad, Lisa E. Wallace

Biological Sciences Faculty Publications

Soil microbiota of the rhizosphere are an important extension of the plant phenotype because they impact the health and fitness of host plants. The composition of these communities is expected to differ among host plants due to influence by host genotype. Given that many plant populations exhibit fine-scale genetic structure (SGS), associated microbial communities may also exhibit SGS. In this study, we tested this hypothesis using Chamaecrista fasciculata, a legume species that has previously been determined to have significant SGS. We collected genetic data from prokaryotic and fungal rhizosphere communities in association with 70 plants in an area of …


The Effect Of Disturbance And Invasion On Fungal And Plant Communities Over An Elevational Gradient, Adam N. Trautwig Oct 2021

The Effect Of Disturbance And Invasion On Fungal And Plant Communities Over An Elevational Gradient, Adam N. Trautwig

Doctoral Dissertations

High-elevation ecosystems are at risk of disruption from the future effects of climate change. Sub-alpine meadows are a source of unique plant populations, intraspecific variation of elevationally extreme populations, and vital sources of fresh water resources. We evaluated the whole fungal communities, arbuscular mycorrhizal fungal (AMF) communities, plant communities, and edaphic variables of sub-alpine meadows in undisturbed, disturbed, and disturbed with a non-native member of the Brassicaceae (Thlaspi arvense) plots. In conjunction with measuring the effects of disturbance on native communities we conducted potting experiments on a dominant grass of sub-alpine meadows (Festuca thurberi). We also …


Dna Barcoding Of The High-Altitude Artemisia And Nepeta Species, Vyacheslav Dushenkov, Csanad Gurdon, Shukhratdzhon Satorov Jun 2021

Dna Barcoding Of The High-Altitude Artemisia And Nepeta Species, Vyacheslav Dushenkov, Csanad Gurdon, Shukhratdzhon Satorov

Publications and Research

DNA barcoding was performed for four medicinal plant species from the mountain region of Tajikistan. The nucleotide sequences for Artemisia sieberi, Artemisia scoparia, Artemisia vulgaris, and Nepeta glutinosa were deposited into the GenBank at the National Center for Biotechnology Information.


A Cophylogenetic Analysis Of Fungus Gardening Ants And Their Symbiotic Fungi, Katherine Beigel Dec 2020

A Cophylogenetic Analysis Of Fungus Gardening Ants And Their Symbiotic Fungi, Katherine Beigel

Biology Theses

Fungus-growing ants (Tribe Attini) and their fungal cultivars share a 50-million-year coevolutionary history. Large scale phylogenetic analyses depict a strong co-phyletic signal among ants and their farmed fungi yet fungus sharing among unrelated ant lineages is somewhat widespread. An overview of sharing has been hampered by a lack of genetic markers that exhibit intraspecific variation and surveys across geographic regions. For example, previous studies have shown similar sequences of the internal transcribed spacer (ITS) region of fungus in different species of Trachymyrmex, suggesting that these ant species are farming the same fungal clone. To examine whether this was a …


Molecular Differentiation Of Astragalus Species And Varieties From The Western United States: The Chloroplast Dna Bridge Between Evolution And Molecular Systematics, Marwa Neyaz, Daniel Cook, Rebecca Creamer Mar 2020

Molecular Differentiation Of Astragalus Species And Varieties From The Western United States: The Chloroplast Dna Bridge Between Evolution And Molecular Systematics, Marwa Neyaz, Daniel Cook, Rebecca Creamer

Poisonous Plant Research (PPR)

Locoweeds are the most widespread poisonous plant problem in the world and have been reported in the Western United States since the 1800s, causing tremendous losses in livestock. Consumption of locoweeds by grazing animals stimulates the neurological disease, locoism, characterized by weight loss, ataxia, and lack of muscular coordination. The name locoweed is used for Astragalus and Oxytropis species known to contain swainsonine, the toxic principle produced by the plant endophytic fungus Undifilum. Astragalus includes 2,500-3,000 species and many varieties that have almost identical morphological characteristics that overlap among species, leading to improper identification. Therefore, the aim of this study …


Further Description Of Hybridization In The Sea Stars Asterias Rubens And Asterias Forbesi Through The Application Of Species-Specific Genetic Markers, Callie Bateson Jan 2015

Further Description Of Hybridization In The Sea Stars Asterias Rubens And Asterias Forbesi Through The Application Of Species-Specific Genetic Markers, Callie Bateson

Honors Program Theses

Hybridization occurs when two separate species reproduce to form hybrid offspring. If the hybrid offspring backcross with individuals of their parental species, the subsequent offspring may have varying amounts of the parental genomes, if viable. Following the retreat of the Last Glacial Maxima 20,000 years ago, two sea star species, Asterias forbesi and A. rubens came into secondary contact in the Gulf of Maine where they began to hybridize. Previous research in our lab studied the frequency of hybridization at four locations along the east coast (Quebec, Nova Scotia, New Hampshire, and New York) using morphological identifications as well two …


Phylogenetic Evaluation Of Series Delimitations In Section Palmata (Acer, Aceroideae, Sapindaceae) Based On Sequences Of Nuclear And Chloroplast Genes, Jianhua Li Mar 2011

Phylogenetic Evaluation Of Series Delimitations In Section Palmata (Acer, Aceroideae, Sapindaceae) Based On Sequences Of Nuclear And Chloroplast Genes, Jianhua Li

Aliso: A Journal of Systematic and Floristic Botany

Acer section Palmata (Japanese maples) is the largest section within the genus; however, series delimitations within section Palmata have not been evaluated in a phylogenetic context. Both maximum parsimony and maximum likelihood analyses of DNA sequence data of nuclear rDNA ITS and chloroplast genes (ndhF, trnL-trnF, and psbA-trnH) from 23 species of Acer section Palmata show that traditional series do not form individual clades. Results from this study support the most recent taxonomic treatment of Acer that does not recognize any series in section Palmata. Nuclear and chloroplast phylogenies are significantly …


Phylogeny Of Abildgaardieae (Cyperaceae) Inferred From Its And Trnl–F Data, Kioumars Ghamkhar, Adam D. Marchant, Karen L. Wilson, Jeremy J. Bruhl Dec 2007

Phylogeny Of Abildgaardieae (Cyperaceae) Inferred From Its And Trnl–F Data, Kioumars Ghamkhar, Adam D. Marchant, Karen L. Wilson, Jeremy J. Bruhl

Aliso: A Journal of Systematic and Floristic Botany

Within the tribe Abildgaardieae, the relationships between Fimbristylis and its relatives have not been certain, and the limits of Fimbristylis have been unclear, with Bulbostylis and Abildgaardia variously combined with it and each other. The relationships and limits of tribes Abildgaardieae and Arthrostylideae and their genera were evaluated across 49 representative species using parsimony and maximum likelihood analyses of ITS (nuclear ribosomal) and trnL–F (plastid) DNA sequence data separately and combined. The evolutionary reconstructions derived from sequences of cpDNA and nrDNA disagree about the position of tribe Arthrostylideae relative to Abildgaardieae; Arthrostylis and Actinoschoenus are either nested within Abildgaardieae …


Systematics Of The Tribe Stipeae (Gramineae) Using Molecular Data, Surrey Jacobs, Randall Bayer, Joy Everett, Mirta Arriaga, Mary Barkworth, Alexandru Sabin-Badereau, Amelia Torres, Francisco Vázquez, Neil Bagnall Dec 2007

Systematics Of The Tribe Stipeae (Gramineae) Using Molecular Data, Surrey Jacobs, Randall Bayer, Joy Everett, Mirta Arriaga, Mary Barkworth, Alexandru Sabin-Badereau, Amelia Torres, Francisco Vázquez, Neil Bagnall

Aliso: A Journal of Systematic and Floristic Botany

Internal transcribed spacer (ITS) sequences have been determined for a wide range of stipoid grasses (Poaceae, Pooideae, Stipeae). Nardus was confirmed as the most appropriate outgroup. Anisopogon is consistently included among the stipoid genera. Lithachne and Oryza form a clade and are clearly not close to Stipeae, and there is no support for including Brachyelytrum within Stipeae. Ampelodesmos and Diarrhena do appear among the core taxa in some analyses, but their positions are unstable and the evidence for retaining them is limited. So far there is inadequate support for rejecting them from Stipeae, so they should be included in any …


A Systematic Approach To Subtribe Loliinae (Poaceae: Pooideae) Based On Phylogenetic Evidence, Pilar Catalán, Pedro Torrecilla, José A. López-Rodríguez, Jochen Müller, Clive A. Stace Dec 2007

A Systematic Approach To Subtribe Loliinae (Poaceae: Pooideae) Based On Phylogenetic Evidence, Pilar Catalán, Pedro Torrecilla, José A. López-Rodríguez, Jochen Müller, Clive A. Stace

Aliso: A Journal of Systematic and Floristic Botany

Loliinae (Poaceae, Pooideae) encompass a large group of genera closely related to Festuca, the largest genus in the subtribe, which as traditionally circumscribed has been shown to be highly paraphyletic. In this investigation we combined molecular and morphological data representing 20 genera of Loliinae and closely related subtribes. Combined analysis of nucleotide sequences from the nuclear ITS and chloroplast trnL–F regions and structural characters recovered a consensus topology that shows Loliinae to be monophyletic and possessing two main clades—the fine-leaved Festuca clade that includes Ctenopsis, Micropyrum, Narduroides, Psilurus, Vulpia, and Wangenheimia, and …


Phylogenetics Of Chloridoideae (Gramineae): A Preliminary Study Based On Nuclear Ribosomal Internal Transcribed Spacer And Chloroplast Trnl–F Sequences, J. Travis Columbus, Rosa Cerros-Tlatilpa, Michael S. Kinney, Maria Elena Siqueiros-Delgado, Hester L. Bell, M. Patrick Griffith, Nancy F. Refulio-Rodriguez Dec 2007

Phylogenetics Of Chloridoideae (Gramineae): A Preliminary Study Based On Nuclear Ribosomal Internal Transcribed Spacer And Chloroplast Trnl–F Sequences, J. Travis Columbus, Rosa Cerros-Tlatilpa, Michael S. Kinney, Maria Elena Siqueiros-Delgado, Hester L. Bell, M. Patrick Griffith, Nancy F. Refulio-Rodriguez

Aliso: A Journal of Systematic and Floristic Botany

The phylogeny of Chloridoideae (Gramineae) was inferred from parsimony analyses of DNA sequences from two genomes—the chloroplast trnL intron, trnL 3' exon, and trnL–F intergenic spacer, and the nuclear ribosomal internal transcribed spacer region (ITS1 + 5.8S + ITS2). Eighty species representing 66 chloridoid genera were sampled, including all but four of the native New World genera. Analyses of the individual and combined data sets were performed. The phylogenies were found to be highly congruent. Of the four tribes and seven subtribes of Chloridoideae sensu Clayton and Renvoize (1986) whose phylogenetic status could be tested with our …


Phylogeny And Biogeography Of Endemic Festuca (Poaceae) From New Zealand Based On Nuclear (Its) And Chloroplast (Trnl–Trnf) Nucleotide Sequences, Kelvin M. Lloyd, Angela M. Hunter, David A. Orlovich, Suzanne J. Draffin, Alan V. Stewart, William G. Lee Dec 2007

Phylogeny And Biogeography Of Endemic Festuca (Poaceae) From New Zealand Based On Nuclear (Its) And Chloroplast (Trnl–Trnf) Nucleotide Sequences, Kelvin M. Lloyd, Angela M. Hunter, David A. Orlovich, Suzanne J. Draffin, Alan V. Stewart, William G. Lee

Aliso: A Journal of Systematic and Floristic Botany

We investigated the phylogenetic relationships of the endemic New Zealand (NZ) species of Festuca (Poaceae, Pooideae) by assessing sequence variation from the nuclear internal transcribed spacers (ITS) and a chloroplast intergenic spacer (trnL–trnF) and by measuring DNA content using flow cytometry. The ITS and trnL–trnF data sets were congruent in showing that the NZ species of Festuca have two origins. One group, containing F. coxii, F. luciarum, F. multinodis, and F. ultramafica, is closely related to Festuca sect. Aulaxyper. The other group includes a clade of five endemic …


Molecular Phylogenetics Of Bromus (Poaceae: Pooideae) Based On Chloroplast And Nuclear Dna Sequence Data, Jeffery M. Saarela, Paul M. Peterson, Ryan M. Keane, Jacques Cayouette, Sean W. Graham Dec 2007

Molecular Phylogenetics Of Bromus (Poaceae: Pooideae) Based On Chloroplast And Nuclear Dna Sequence Data, Jeffery M. Saarela, Paul M. Peterson, Ryan M. Keane, Jacques Cayouette, Sean W. Graham

Aliso: A Journal of Systematic and Floristic Botany

We conducted a phylogenetic analysis to characterize relationships among Bromus and test the monophyly of five of the seven morphologically distinct groups within Bromus (Poaceae: Pooideae) that have been treated as sections, subgenera, or genera. We sequenced the chloroplast trnL (UAA) intron, the 3'-end of the chloroplast ndhF gene, and the internal transcribed spacers (ITS) of the nuclear ribosomal DNA region for 46 species that represent a large proportion of the morphological and geographical diversity in the genus. Independent analyses of plastid and nuclear ribosomal data identified several lineages in Bromus, but there is some evidence of …