Ecology and Evolutionary Biology Commons^™

Impacts Of Herbicide Drift On The Plant-Rhizobia Mutualism Is Dependent On Genetic Variation In Both Mutualists, Veronica Iriart

2023 Midwest Ecology & Evolution Conference

The symbiotic mutualism between plants and rhizobial bacteria that occurs within root nodules, wherein plants supply rhizobia with carbon in exchange for biologically fixed nitrogen, provides critical ecosystem services via plant growth promotion and nutrient cycling. However, it is unknown how contemporary anthropogenic stressors such as pesticide pollution affects this important ecological relationship. We grew red clover (Trifolium pratense) from 17 genetic families in the greenhouse either alone or in symbiosis with one of two rhizobial strains. We exposed half to a sublethal rate of the herbicide dicamba, equivalent to what wild plants experience when herbicides ‘drift’ off-target …

The Effects Of Imposed Drought And Nutrient Availability On Soil Microbial Biomass And Enzyme Activity In A Long-Term Restored Grassland, Ashley A. Wojciechowski

2023 Midwest Ecology & Evolution Conference

Restoring agricultural lands back to grassland aims to reverse many negative impacts of agriculture on the ecosystem including nutrient export from soil and depletion of soil carbon. Planting prairie on agricultural soil promotes recovery of soil microbial biomass and their enzymes, which create demand for nitrogen by microbes to reduce the rate of nutrient cycling and loss. A complicating factor in the development of a restored grassland the influence of several anthropogenic global changes including increasing frequency of drought and nutrient enrichment. Drought has been found to negatively affect microbial biomass, soil enzyme activity and nutrient cycling. Nutrient (often N) …

Investigating Pollinator Mismatch In Cardamine Concatenata And Claytonia Virginica, Nada Elgousi

2023 Midwest Ecology & Evolution Conference

Global warming is gradually affecting the local climate causing pollinators and plants to change their emergence and flowering times. This can potentially cause a phenology mismatch between pollinator activity and open flowers leading to limitation in seed production in plants. However, other factors such as intense predation or competition can also shift phenology. As part of a preliminary study exploring the mechanisms of phenology mismatch, Cardamine concatenata and Claytonia virginica, were observed to determine if there is a phenology shift due to competition with an invasive plant that flowers early and overlaps these two native species. In early spring, …

Does The Genome Of A Putatively Specialist Moth (Schinia Gracilenta) Contain A Signal Of The Indigenous Cultivation Of Its Host Plant (Iva Annua), Wren Renquist

2023 Midwest Ecology & Evolution Conference

When one species depends on one another for survival, they may have shared demographic histories. Further, when symbionts have shorter life spans, their rate of evolution may be quicker than that of their hosts. For this reason, detecting a recent demographic signal in a symbiont’s genome may often be easier than directly studying the host.

Here I study the slender flower moth (Schinia gracilenta) and its host plant, sumpweed (Iva annua). Sumpweed is thought to have been cultivated in North American ca. 5000 BC - AD 1700 and its range may have been expanded during this time. I collected S. …

Decadal Patterns Of Adaptation And Trait Variation Of The Dominant Prairie Grass Across The Midwest Rainfall Gradient And Response To Drought, Jack Sytsma

2023 Midwest Ecology & Evolution Conference

Frequent droughts are predicted to impact grasslands but intraspecific variation in response to drought is not well-known. Big bluestem (Andropogon gerardii) is a grass distributed across the Midwest rainfall gradient (MAP500-1200mm/yr) giving rise to climate-adapted ecotypes. This dominant grass is important for cattle forage and conservation. Reciprocal gardens were established in 2009 in ecological communities (driest to wettest: Colby, Hays, Manhattan, KS to Carbondale IL) and measured for over a decade. Rainfall was reduced using rainout shelters in three sites. Cover and biomass were monitored in plots over time, and height, width, photosynthetic rate, SPAD, and reproductive phenology …

Effects Of Extra-Pair Paternity On Provisioning Effort And Nest Defense In Male House Wrens (Troglodytes Aedon), Avery Dart

2023 Midwest Ecology & Evolution Conference

Some animals form socially monogamous pair bonds in which a male and female mate and raise young together. However, individuals within a socially monogamous pair bond may still mate with other individuals; this mating is defined as extra-pair mating (EPM). Extra-pair paternity (EPP) occurs when offspring arise from EPM. In wild birds, EPM is approximated by EPP. Males benefit from EPP by siring additional offspring without any investment aside from sperm, but the benefits of EPM for females are less clear. Females may gain fertility assurance, access to better foraging grounds, and nest protection from males, as males are incentivized …

The Contribution Of Sexual Selection To Trait Divergence In Rhagoletis Flies, Sophia Anner

2023 Midwest Ecology & Evolution Conference

Sexual selection, or the process that favors traits for acquiring mates, is a powerful mechanism driving sexual trait variation like vivid fish colors, melodic cricket songs, and elaborate jumping spider dances. Sexual selection can also drive divergence between populations and speciation, generating an immense diversity of species. Flies in the Rhagoletis genus exhibit different coloration, size, behavior, and most conspicuously, wing patterns between species. These traits are employed when flies communicate with each other, including during mating interactions, and yet the role of sexual selection in trait variation has been sorely understudied. I conducted mating trials and morphological analysis to …

Giant Mice On Small Islands: Mechanisms Of Gigantism In Peromyscus Maniculatus Gulf Island Populations, Rachel A. Berg

2023 Midwest Ecology & Evolution Conference

Island rodent populations frequently exhibit island gigantism, presenting with larger body sizes in comparison to mainland counterparts. Proposed mechanisms to explain gigantism include benign island habitats and lack of mainland predators and competitors. However, island populations tend to be denser than mainland populations, increasing conspecific competition. With extrinsic sources of pressure largely missing from island habitats, conspecific competition may become a selecting force for larger body sizes in island populations. Here we examine presence of gigantism in North American deermouse (Peromyscus maniculatus) populations throughout the Gulf Islands of British Columbia, Canada and assess if population density is an underlying …

Anthropogenic Influences Of Play In The Green Monkeys Of Barbados, Rose Amrhein

2023 Midwest Ecology & Evolution Conference

There are multiple theories behind the evolutionary origins and functions of play behavior in the animal kingdom, all with varying levels of support. Some argue play originated as a byproduct of individuals having excess energy in a resource-rich environment while others argue it arose as a way for young individuals to enhance the physical and mental skills needed to survive into adulthood. Many believe that both these theories are coexisting and applicable to some degree. However, previous play studies usually focused on one group of organisms, failing to consider how animals’ environment directly impacts the structure of their play and …

Evaluating Anti-Predator Behavior In A Captive Elk (Cervus Canadensis) Herd, Jordan L. Tandy

2023 Midwest Ecology & Evolution Conference

Predator-prey interactions drive decisions of either counterpart, meaning predators and the presence of perceived dangers, such as scent markings or calls, are capable of altering an organism’s behavior. On a spatial scale, risk and cost-benefit tradeoffs related to predation pressures create a landscape of fear (LOF), where prey avoid areas they associate with risk. Certain periods of time, such as birthing seasons, pose even greater risk to prey species. Research suggests that captive and/or historically captive animals tend to respond less effectively to predation than native populations that regularly experience threats of predation. I have gathered behavioral data on a …

Relationships Between Alternative Visual Methods To Quantify Tree Reproduction, Riley Adams

2023 Midwest Ecology & Evolution Conference

Many tree species have extremely high variability in their magnitude of reproduction over space and time. Annual seed crop variation in trees affects forest regeneration, and the seeds influence animal reproduction and wildlife population dynamics in numerous species (e.g., birds, mammals, and insects). The number of reproductive structures on trees is commonly estimated by varying visual counting methods, from categorical classifications as done by the National Phenology Network, to timed counts and counts of all visible reproductive structures. Different methods may produce different findings, and with recent multi-species databases, constructed on tree reproduction and for future projects, variation across methods …

Does Stress Physiology Mediate Disease Resistance In The Arizona Tiger Salamander (Ambystoma Mavortium Nebulosum)?, Megan Zerger

2023 Midwest Ecology & Evolution Conference

Amphibian biodiversity has greatly diminished in recent years due to disease. Panzootic pathogenic fungi Batrachochytrium dendrobatidis (Bd) causes the deadly disease chytridiomycosis (chytrid), a primary driver of catastrophic global amphibian declines and extinctions. The pathogenesis of chytrid is still unclear, as certain species and individuals within a species are differentially affected. Susceptibility and mortality of Bd are influenced by prolonged chronic stress. Prolonged glucocorticoid activity deleteriously affects many of the same physiological processes as Bd infections, and corticosterone is the primary glucocorticoid released by amphibians in response to stress. Thus, the objective of our study is to assess …

Adaptive Plasticity Of Coloration In Response To Environmental Change, Karissa Coffield

2023 Midwest Ecology & Evolution Conference

When rapid environmental changes occur, different selective forces can create phenotypic trade-offs in which a trait can provide fitness benefits or costs under different environmental conditions. Amphibians are particularly vulnerable to environmental change, and previous research has revealed that some species will plastically respond to variation in temperature and ultra-violet radiation (UVR) by altering their coloration. Divergent selection on coloration may change with elevation and climate induced shifts in temperature because high temperatures are likely to result in lighter color morphs but as elevation increases, UVR exposure increases leading to the prediction that darker color morphs will be more common. …

Trait Variation In Bryophytes At Karst Cave Entrances, Chenoa Briggs

2023 Midwest Ecology & Evolution Conference

Cave entrances are critical ecosystems in their role as ecotones between the interior cave environment and the surrounding external environment. Bryophytes are highly sensitive to environmental changes and many species found at caves are specialists to those habitats. This study aims to identify how functional traits of bryophytes are affected by the cave environment. Bryophytes and environmental variables were sampled from six cave entrances. Functional trait data was collected from literature. The data from this study supplies information on the functional traits of bryophytes and can provide insight into the effects of environmental pressures on community assembly.

Seroton-Ing Down The Stress: Serotonergic Activity In Response To Predators In Threespined Stickleback (Gasterosteus Aculeatus), Michaela M. Rogers

2023 Midwest Ecology & Evolution Conference

Animals constantly face stress in the environment due to rapid human-induced changes. A strong interest lies in the evolution of coping mechanisms that can help organisms deal with and respond to these stressors. Serotonin is a neurotransmitter that is highly conserved across taxonomic groups and is upregulated in response to a broad range of stressors, including predator exposure. Serotonin upregulation can help organisms cope with the threat of a predator by decreasing fear and anxiety. Previous studies have shown an increase in serotonergic activity following acute predator exposures in mice, rats, and fish after only one exposure, but less is …

Assessing The Health And Behavioral Effects Of Turtle Racing On Eastern Box Turtles (Terrapene Carolina), Michelle K. Weaver

2023 Midwest Ecology & Evolution Conference

Turtle racing involves the public capturing largely wild-caught box turtles (Terrapene spp.) and racing them at standalone events or at races incorporated into larger family events (e.g., festivals). These turtle races are legal in most states because of a lack of regulations and/or funding to enforce regulations that may exist. Moreover, we lack information on the potential effects (e.g., disease transmission, survival, movements, and general health) of races on the turtles that could help support the implementation of regulations. Our objective was to understand the short-term and long-term health and behavioral effects of turtle races on eastern box turtles (Terrapene …

Endophytic Fungi And Their Competitive Hierarchy And Effects Of Changing Environmental Nitrogen., Haley G. Bryant, Kylea R. Garces

2023 Midwest Ecology & Evolution Conference

Endophytes are fungi that live symbiotically within the roots of plants. These fungi can serve different functions within the roots, and are thought to form a competitive hierarchy that allows them to coexist. It is not well established how specific species act within this endophytic dynamic, especially with each other. This experiment looked to better understand these relationships through Grime’s C-S-R model; growing the extracted fungal species in different treatments: control, stress, low nitrogen, high nitrogen, and comparing each growth rate and biomass over a two-week period. The species looked at in this study were extracted from dominant dune-building grass …

A Novel Approach To Scoring Rhythmic Variation In Evolving White-Throated Sparrow (Zonotrichia Albicolis) Songs, Taylor Hiers

2023 Midwest Ecology & Evolution Conference

Studies show that the White-throated Sparrow's (Zonotrichia albicolis) song is evolving across North America. Previously, most songs ended in a series of "triplets" consisting of three notes. Sometime after the 1960s, a new variant arose in western Canada that ended in a series of "doublets" consisting of two notes. A recent study found that the doublet variant has since spread rapidly eastward across the continent, replacing the triplet variant. We set out to document this shift in southwest Missouri, which is part of the sparrow's wintering range. We found that triplets were significantly more common than doublets, indicating …

Late Cretaceous Marine Vertebrates From The Tarrant Formation, Tarrant County, Texas U.S.A, Aaron Johnson, Kenshu Shimada

2023 Midwest Ecology & Evolution Conference

The Tarrant Formation (middle Cenomanian: ca. 96 million years ago) is a stratigraphic member of the Upper Cretaceous Eagle Ford Group in Texas, U.S.A., and consists of calcareous sandstone formed near the East Texas Embayment of the Late Cretaceous Western Interior Seaway, an epicontinental seaway in North America. We examined vertebrate fossils from the Tarrant Formation based on specimens collected through surface collecting and bulk sampling from three localities in Tarrant County, Texas, including the type locality of a pachyrhizodontid fish, Polcynichthys lloydhilli. Our preliminary examination shows that the fossil fauna includes shark species such as Ptychodus anonymous, Archaeolamna sp., …

Habitiat Structure And Ecomorphology Of Fishes In Neotropical Blackwater Creeks., Devya Hemraj-Naraine

2023 Midwest Ecology & Evolution Conference

Freshwater ecosystems provide habitat for a diversity of aquatic flora and fauna. However, anthropogenic activities like mining and pollution alters aquatic habitats and the ecosystem services they provide. In South America, which hosts one quarter of all freshwater fish species, aquatic habitats are under considerable encroachment from human development. We surveyed nine blackwater creeks tributary to the Demarara River along a major Highway in Guyana to determine whether fish morphology and community structure correlate with habitat characteristics among sites with different degrees of human impact. Twenty-three morphological traits were measured on 3-5 adult specimens of each species. The most abundant …

Pollinators And Flowering Resources In Louisville Urban Green Spaces, Sarah Hobbs

2023 Midwest Ecology & Evolution Conference

Urban Greenspaces can appear in many different forms such as parks, urban orchards, and community gardens. These spaces provide important ecosystem services such as enhanced biodiversity, improved soil health, and increased carbon sequestration. Greenspaces such as parks and community gardens have been relatively well studied in the last few decades compared to urban orchards, despite the multitude of ecosystem services that can be provided or enhanced by these orchards. We hypothesize that urban orchards will provide the optimal conditions for pollinator communities because urban orchards normally have the greatest variety of vegetation and flowering resources, which is positively correlated with …

Herbarium Records Reveal Sex-Specific Effects Of Climate Warming On Phenology Of Fragaria Virginiana, Ethan R. Richardson

2023 Midwest Ecology & Evolution Conference

Plant populations tend to be hermaphroditic, but in some cases can diverge into a dioecious sexual system, or one that possesses both male and female members. Prior studies show a correlation between sex systems evolution such as these and ecological variables. These variables impose challenges on plants' ability to propagate and in a dioecious population, males and females have been seen to respond differently to different environmental contexts. What is still being studied is the degree to which biogeography contributes to the sexual differentiation of dioecious species. While evidence supports the idea of a latitudinal gradient contributing to sexual differentiation, …

How Accurately Structure Can Identify Hybrids When Comparing Different Numbers Of Species And Marker Types And Numbers, Chloe Hendrikse

2023 Midwest Ecology & Evolution Conference

Hybridization is a common problem in conservation. It can be difficult to identify hybrid individuals but doing so is often necessary in order to make conservation recommendations. For example, botanical gardens and seed banks want to identify if individuals are hybrids of two or more species to determine if offspring could be used for conservation or restoration, and to analyze the risk of possible extinction via introgression. Our project looks at how well the program STRUCTURE can detect and cluster hybrids, their parent species, and other pure species, which we created using simulations. We build on past studies, which typically …

Functional Implications Reflected In Neurocranial Morphology Of Lamniform Sharks (Elasmobranchii: Lamniformes), Jake J. Wood

2023 Midwest Ecology & Evolution Conference

Lamniform sharks (Elasmobranchii: Lamniformes) display diverse habitat preferences and lifestyles. Based on museum specimens and published illustrations, we examined the proportion of the anteroposterior length of the rostral, olfactory, optic, and otic regions with respect to the neurocranial length in each lamniform species as a proxy of relative importance of each sensory region (electroreception, olfaction, vision, and ‘coordination’) to its lifestyle. We show that: 1) Carcharias and Megachasma (ragged-tooth and megamouth) have short rostral and large otic regions indicative of slower-swimming pelagic lifestyles with elevated coordination for balancing in the water column; 2) Alopias pelagicus, A. vulpinus, …

Leaf Litter Arthropod Communities Not Responsive To Nutrient Pulse Subsidies, Andrew R. Seiler, Stephen P. Yanoviak

2023 Midwest Ecology & Evolution Conference

Spatial subsidies are the asymmetric movement of resources from a donor habitat to a distinct recipient habitat. Arthropod rain, i.e., the fall of arthropods from tree crowns to the forest understory, is a potentially important subsidy link between canopy food webs and detritus-based litter food webs. We added homogenized fragments of Blaptica dubia cockroaches in high (12g m-2) and low (2g m-2) concentrations to replicated quadrats of leaf litter as a pulse subsidy in a temperate forest. Controls were: 1) zero subsidy and 2) addition of foam pellets in place of cockroach fragments. Litter in each quadrat was subsampled 2, …

Sexual Selection, Female Body Size, And Fecundity In Drosophila Melanogaster, Calvin Herrick

2023 Midwest Ecology & Evolution Conference

In Drosophila melanogaster it is well established that male sexual preference emphasizes high fecundity in females and females with larger body sizes. It is hypothesized that male preference for large females is due to a positive correlation between body size and fecundity. Here, we take advantage of a long-running experiment, where lineages of flies were made significantly larger (L-lineages) and smaller (S-lineages) during 400+ generations of artificial selection. In the first part of this study, we measured female Drosophila fecundity, based only on differences in evolved size and found substantial differences in fecundity. In the second part of this study, …

Habitat Characteristics And Winter Use Of Woodpecker-Excavated Tree Cavities, Adrianna Yoder

2023 Midwest Ecology & Evolution Conference

Avifauna are in decline in North America, including red-headed woodpeckers (Melanerpes erythrocephalus). Red-headed woodpeckers are negatively impacted by urbanization, which has decreased their ideal habitats of oak-savannas and through the management of urban trees there has been a reduction in the amount of tree decay, a necessary feature of suitable trees that woodpeckers can make cavities in. Cavities are created and used as nests in the breeding season (spring/summer) and as roost-sites in the non-breeding season (fall/winter). In the Chicagoland region, during the fall some red-headed woodpeckers migrate while others do not and remain overwinter, making them facultative …

Air Pollution Is Associated With Increased Stress In Urban Songbirds, Reagan Earlywine

2023 Midwest Ecology & Evolution Conference

Air pollution has been suggested to be a major driver of decline in native bird populations. Understanding how air pollution affects avian physiology and how air pollution exposure may be reduced may help develop effective conservation solutions. Here we tested whether elevated air pollution exposure was associated with increased physiological stress response in free-living urban American robins, and whether urban vegetation mitigated this effect. Specifically, we asked whether the concentration of the stress-related glucocorticoid hormone corticosterone was predicted by exposure to two metrics of air pollution: nitrogen dioxide (NO₂) and ultrafine particulates (UFPs); and two metrics of green …

Recognition Of Conspecifics Under Elevated Turbidity, Ryan L. Gaynor

2023 Midwest Ecology & Evolution Conference

A key component of sociality is the ability of individuals to collect relevant social information about groupmates. Human-induced rapid environmental change (HIREC) can interfere with the collection of accurate social information by altering organisms’ sensory environment. In aquatic ecosystems anthropogenic disturbances can often cause an increase in water turbidity, thereby altering the visual environment of a body of water. This change to the sensory environment can degrade or obscure visual information used by individuals to mediate social behavior and recognize conspecifics. Neolamprologus pulcher is a cooperatively breeding cichlid that lives in small groups and utilize a variety of visual cues …

Spider Web Morphology Is Associated With Damage By Falling Woody Debris, Abigail Nienaber

2023 Midwest Ecology & Evolution Conference

Web-building spiders are important predators in most terrestrial habitats. Web damage is common and presents potential fitness consequences for the spider. Falling woody debris is likely a major source of web damage for forest-dwelling spiders, especially Leucauge venusta, a widespread species that builds horizontal webs. We quantified damage area, general dimensions, and spatial orientation of L. venusta webs in two Kentucky forests. We also measured woody debris fall from the canopy. We experimentally damaged webs with falling debris in the lab to test the hypothesis that L. venusta change their web orientation following disturbance. The relative area of webs …