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2023

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Articles 391 - 420 of 1079

Full-Text Articles in Ecology and Evolutionary Biology

Effects Of Nonnative Plants On Space-Use In Eastern Box Turtles, Emily Ilene Crago May 2023

Effects Of Nonnative Plants On Space-Use In Eastern Box Turtles, Emily Ilene Crago

Graduate Theses

Introduction of nonnative plants outside their natural range has caused widespread reduction in the abundance and diversity of native plant species. Nonnative plants typically form dense, often monotypic, thickets that affect the ability of animals to find food, reproduce, avoid predation risk, and thermoregulate. By doing so, nonnative plants have the potential to displace resident animals from areas otherwise suitable as animal habitat. However, limited studies have investigated whether resident animals avoid areas dominated by nonnative plants. This study investigated impacts of nonnative plants on eastern box turtles (Terrapene carolina carolina), a species that has been declining throughout their range. …


Exploring The Relationship Between Dreissenids And The Deep Chlorophyll Layer In The Laurentian Great Lakes, Lara E. Hargrave May 2023

Exploring The Relationship Between Dreissenids And The Deep Chlorophyll Layer In The Laurentian Great Lakes, Lara E. Hargrave

Great Lakes Center Masters Theses

In the Laurentian Great Lakes, two invasive bivalves Dreissena polymorpha and Dreissena rostriformis bugensis have induced a number of changes, including increased water transparency, alterations in nutrient cycling, decreased chlorophyll concentrations, and changes in phytoplankton community composition and structure. These changes are likely to have implications for the deep chlorophyll layer (DCL), a feature characterized by phototrophic organisms reaching abundance at light levels far below ideal for photosynthesis. Though the impacts of D. polymorpha and D. r. bugensis in the Great Lakes have been studied for at least the last three decades, little is known about how these ecosystem engineers …


The Effects Of Recent Climate Change On Spring Phenology, With A Special Focus On Patterns Of Bee Foraging, Michael Stemkovski May 2023

The Effects Of Recent Climate Change On Spring Phenology, With A Special Focus On Patterns Of Bee Foraging, Michael Stemkovski

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The date on which plants flower and on which bees begin to pollinate varies year-to-year depending on differences in weather. This seasonal timing is known as phenology, and it is already clear that climate change has pushed the spring phenology of many species earlier by increasing temperatures. This is particularly clear in flowering plants, but studying how and why the phenology of pollinators is shifting is more difficult. Most flowering plants rely on pollinators such as bees for their reproduction, and most bees rely on flowers for their sustenance, so bee and flower phenology has to overlap for the crucial …


Neonate Atlantic Sharpnose Shark (Rhizoprionodon Terraenovae) Relative Abundance And Body Condition In Two South Carolina Estuaries Varying In Urbanization, Rileigh E. Hawk May 2023

Neonate Atlantic Sharpnose Shark (Rhizoprionodon Terraenovae) Relative Abundance And Body Condition In Two South Carolina Estuaries Varying In Urbanization, Rileigh E. Hawk

Electronic Theses and Dissertations

Urbanization near estuaries has been shown to affect the growth and survival of juvenile sharks using the system as a nursery. North Inlet and Murrells Inlet, South Carolina, are similarly-sized, tidally-dominated, bar-built estuaries with extensive Sporobolus-lined tidal creeks but differ in degree of human impact. Previously, Murrells Inlet was shown to have a lower abundance and diversity of large sharks than North Inlet and Atlantic Sharpnose Sharks (Rhizoprionodon terraenovae) were shown to use North Inlet as a primary nursery. To examine potential differences in neonate shark abundance and growth between a developed estuary, Murrells Inlet, and a protected estuary, North …


An Integrative Approach To Modelling Human-Wildlife Coexistence Landscapes In The Northern Great Plains, Keifer Titus May 2023

An Integrative Approach To Modelling Human-Wildlife Coexistence Landscapes In The Northern Great Plains, Keifer Titus

All Dissertations

Restoring wildlife populations in a human dominated world requires a deep understanding of the ecological conditions required for species persistence as well as the human social factors that influence restoration outcomes. Until recently, the majority of prior research has focused on understanding the ecological conditions and human social processes that contribution to wildlife restoration success separately, and often assign a higher value to ecological factors. I studied the human dimensions surrounding ongoing wildlife restoration efforts in the Northern Great Plains (NGP) of Montana to better understand how human social factors can affect and inform restoration efforts for a variety of …


Acetate Metabolism In The Fungal Pathogen Cryptococcus Neoformans, Oly Ahmed May 2023

Acetate Metabolism In The Fungal Pathogen Cryptococcus Neoformans, Oly Ahmed

All Dissertations

Cryptococcus neoformans is an environmental basidiomycetous fungus with a worldwide distribution and a wide range of habitats. Inhalation of the desiccated yeasts or spores of C. neoformans often leads to opportunistic pulmonary infections in immunocompromised individuals, and in severe cases causes lethal meningitis following hematogenous dissemination. During infection, depending on the tissue and disease state, the invading fungi experience a range of nutrient microenvironments within the host body. As a result, rapid metabolic adaptations geared towards efficient utilization of carbon sources alternative to glucose become one of the prime determinants of survival and growth for the pathogen. Incidentally, cryptococcal infection …


Variations In Thermoregulation Strategies Of Northern And Southern Populations Of Mischocyttarus Mexicanus, Taylor Guild May 2023

Variations In Thermoregulation Strategies Of Northern And Southern Populations Of Mischocyttarus Mexicanus, Taylor Guild

Honors Theses

Mischocyttarus mexicanus is a species of eusocial paper wasp that is found across much of the Southeastern coastal region of the United States. Contrasting wintering strategies have been seen in northern versus southern populations. Anecdotally, northern populations overwinter by clustering on top of palmetto fronds, their preferred nesting substrate, in a sort of "taco" shape, while southern populations stand on their nests that hang on the underside of the palmetto frond throughout the year. I tested three hypotheses related to the adaptive value of northern over-frond clustering behavior: 1) Over-frond clustering is thermoregulation related, meaning it would be temperature driven. …


Drivers Of White-Tailed Deer (Odocoileus Virginianus) Behavior, Survival, And Population Growth In The Piedmont Of South Carolina, Michael Muthersbaugh May 2023

Drivers Of White-Tailed Deer (Odocoileus Virginianus) Behavior, Survival, And Population Growth In The Piedmont Of South Carolina, Michael Muthersbaugh

All Dissertations

Prey species adjust behaviors in response to various stimuli, responding to both top-down and bottom-up pressures. Large herbivores must attempt to avoid predation either through adjusting behaviors or seeking spatial or temporal refugia, which can scale up to influence individual fitness and ultimately population dynamics. Specifically, predation risk has a great potential to influence ungulate populations by impacting behaviors and survival. Our objective was to quantify ungulate behavioral and population response to a regionally important predator through a case study – white-tailed deer (Odocoileus virginianus) responses to coyotes (Canis latrans) on private lands in the Piedmont …


Rattus Norvegicus As A Biological Detector Of Clandestine Remains And The Use Of Ultrasonic Vocalizations As A Locating Mechanism, Gabrielle M. Johnston May 2023

Rattus Norvegicus As A Biological Detector Of Clandestine Remains And The Use Of Ultrasonic Vocalizations As A Locating Mechanism, Gabrielle M. Johnston

Master's Theses

In investigations, locating missing persons and clandestine remains are imperative. One way that first responder and police agencies can search for the remains is by using cadaver dogs as biological detectors. Cadaver dogs are typically used due to their olfactory sensitivity and ability to detect low concentrations of volatile organic compounds produced by biological remains. Cadaver dogs are typically chosen for their stamina, agility, and olfactory sensitivity. However, what is not taken into account often is the size of the animal and the expense of maintaining and training the animal. Cadaver dogs are typically large breeds that cannot fit in …


Sand Escarpments As A Potential Barrier To The Nesting Distribution Of Sea Turtles, Dakota Ronco May 2023

Sand Escarpments As A Potential Barrier To The Nesting Distribution Of Sea Turtles, Dakota Ronco

Undergraduate University Honors Capstones

Sea turtles are a part of both the marine and terrestrial ecosystems as primary and secondary consumers. By laying nests on the beaches and migrating across the ocean, sea turtles are significant in maintaining the nutrient (nitrogen and phosphorus) flux between terrestrial and marine ecosystems. Nests bring nutrients into the sand by decomposing unhatched eggs and egg remains. The distribution of their nests is significant in getting insights into the population density of sea turtles and what drives the nesting location, which ultimately regulates nutrient flux. The location of their nests is driven by sandy beach ecosystem structures, especially active …


Relationships Between Horizontal And Vertical Movements Of Shortfin Mako Sharks (Isurus Oxyrinchus) In The Western North Atlantic Ocean, Katie Gheysen May 2023

Relationships Between Horizontal And Vertical Movements Of Shortfin Mako Sharks (Isurus Oxyrinchus) In The Western North Atlantic Ocean, Katie Gheysen

Senior Honors Projects

Highly mobile pelagic predators, such as tuna, swordfish, and sharks, often undergo changes in depth over a 24-h period and exhibit diel vertical migration (DVM), presumably to maximize foraging efficiency. Studies on the diving behavior of shortfin mako sharks (Isurus oxyrinchus) have been inconclusive as to whether this species consistently exhibits DVM. We investigated relationships between horizontal and vertical movements in shortfin mako sharks by using location and depth data collected from SPLASH tags and pop-up satellite archival tags (PSAT). A state-space model was applied to location data derived from SPLASH tags to generate continuous tracks and to …


Trace Dna Detection Using Diamond Dye: A Recovery Technique To Yield More Dna, Leah Davis May 2023

Trace Dna Detection Using Diamond Dye: A Recovery Technique To Yield More Dna, Leah Davis

Master's Theses

This study aspires to find a new screening approach to trace DNA recovery techniques to yield a higher quantity of trace DNA from larger items of evidence. It takes the path of visualizing trace DNA on items of evidence with potential DNA so analysts can swab a more localized area rather than attempting to recover trace DNA through the general swabbing technique currently used for trace DNA recovery. The first and second parts consisted of observing trace DNA interaction with Diamond Dye on porous and non-porous surfaces.

The third part involved applying the Diamond Dye solution by spraying it onto …


Assessing The Utility Of High-Throughput Phenotyping For Ecological Applications, Annabree Corlew May 2023

Assessing The Utility Of High-Throughput Phenotyping For Ecological Applications, Annabree Corlew

Honors Theses

A phenotype describes the expressed traits of an organism. These traits can provide insight into interactions between an organism and its environment, making them a key focus of ecological research. But the process of phenotyping traits can be tedious to collect for large-scale experiments. High-throughput phenotyping software has the ability to speed up the phenotyping process by allowing multiple traits to be phenotyped at once. However, the utility of this relatively new and continually developing computational tool is still being assessed, especially beyond applications focused on agricultural crops and grasses. I investigated the accuracy and efficiency of the open-access high-throughput …


Species Diversity Of Coleoptera Populations In An Urban Garden In Chattanooga, Tennessee, Mallory Anderson May 2023

Species Diversity Of Coleoptera Populations In An Urban Garden In Chattanooga, Tennessee, Mallory Anderson

Honors Theses

Insects are an essential part of the function of ecosystems. The majority of entomological faunistic research has been conducted primarily in rural areas due to high diversity, space for bulky traps, and less outside influence from human activities. However, the need for time-series insect biodiversity data, in rural areas and urban areas alike, is paramount in the wake of our current sixth mass extinction event. Previous research has shown that the greater Chattanooga area is comprised of a diverse population of insects in the Coleoptera (beetles) order, and as shown in the University of Tennessee at Chattanooga’s (UTC) Insect Collection. …


Impacts Of Bacterial Evolution On Host Lethality In Drosophila, Andrew Preston May 2023

Impacts Of Bacterial Evolution On Host Lethality In Drosophila, Andrew Preston

College of Arts & Sciences Senior Theses

Evolution is the process by which species change their genetic traits, such as the pathogenicity of bacteria, over time in response to changes in their environment. Although the genetic mechanisms underlying many evolutionary processes have been revealed, it is still not well understood how opportunistic pathogens, such as Pseudomonas aeruginosa, become virulent. The overall goal of this thesis is to test the Coincidental Evolution Hypothesis, which proposes that the virulence of opportunistic pathogens evolves coincidentally as a by-product of their interaction with their natural predators. I hypothesized that the virulence of ancestral Pseudomonas aeruginosa changes over time if it co-evolves …


It’S Free Real Estate: Benthic Assemblage Composition On Bluehead Chub Nests In Southeastern Streams, Isabel Evelyn May 2023

It’S Free Real Estate: Benthic Assemblage Composition On Bluehead Chub Nests In Southeastern Streams, Isabel Evelyn

All Theses

Positive interactions such as facilitative habitat modification are often strong drivers in structuring communities. The Bluehead Chub (Nocomis leptocephalus) is a common fish in Piedmont streams of the southeastern USA that builds gravel mound spawning nests that other native fish species utilize as critical spawning substrate. These nests are colonized by other freshwater benthic organisms, though few studies have investigated these colonizing assemblages. The goal of this study was to quantify differences in benthic assemblage composition and abundance between Bluehead Chub nests and unmodified streambed substrate as well as relative taxa microhabitat associations in relation to stream reach-wide …


An Investigation Into The Origin And Molecular Mechanism Of Species-Specific Craniofacial Sexual Dimorphism In Lake Malawi Cichlids, Ashleigh Brandon May 2023

An Investigation Into The Origin And Molecular Mechanism Of Species-Specific Craniofacial Sexual Dimorphism In Lake Malawi Cichlids, Ashleigh Brandon

All Theses

Explaining the underlying source that generates the phenotypic diversity ubiquitous in nature has been a major goal of biologists since the time of Darwin. In addition to the phenotypic variation that exists between different species, another potent source of morphological diversity is found within a species, in the form of phenotypic differences that are specific to males and females, termed sexual dimorphism. The largest craniofacial bone, the mandible, exhibits such extreme sexual dimorphism that it may be used to forensically determine sex. This dimorphism occurs in most of the craniofacial complex and contributes to sex-biased disease frequencies, such as a …


Hibernating Tricolored Bats At A Southeastern Recreation Site: Microsite Use And Public Perceptions, Rebecca Brown May 2023

Hibernating Tricolored Bats At A Southeastern Recreation Site: Microsite Use And Public Perceptions, Rebecca Brown

All Theses

High bat mortality from white-nose syndrome (WNS) has increased the need to manage hibernation sites. Management decisions should be based on science, and when sites are accessible to the public, also need to consider human dimensions. We used Stumphouse Tunnel, a recreation site and tricolored bat (Perimyotis subflavus) hibernaculum in northwestern South Carolina, as a case study to understand potential conflicts between conservation and recreation. The population declined by 90% after the arrival of white-nose syndrome (WNS), followed by stabilization and slight increases. Because the increase was associated with more bats roosting in the publicly accessible section, a potential …


Immediate Response Of Bats To Prescribed Fire And Impact Of Experiences On Women's Self-Image In Natural Resources Professions, Zebria Hicks May 2023

Immediate Response Of Bats To Prescribed Fire And Impact Of Experiences On Women's Self-Image In Natural Resources Professions, Zebria Hicks

All Theses

To inform use of prescribed fire management practice in the southeastern US, we studied its impact on bats, which are important and at-risk species. We evaluated if prescribed fire had a positive, neutral, or negative effect on bat activity in the two weeks following the burns. We recorded bat activity after prescribed burns in February and March 2022 in northwestern South Carolina in select hardwood and pine stands and control sites ≥ 500 m from burn boundaries. We measured insect abundance, canopy cover, basal area, and understory density at each site. We recorded 687 passes during our 45-day study period. …


Growth, Mortality, And Movement Of Endemic Bartram's Bass And Invasive Alabama Bass In Upper Savannah River Basin Tributaries, Tyler Zumwalt May 2023

Growth, Mortality, And Movement Of Endemic Bartram's Bass And Invasive Alabama Bass In Upper Savannah River Basin Tributaries, Tyler Zumwalt

All Theses

Bartram’s Bass are an endemic to the Upper Savannah River basin in North Carolina, South Carolina, and Georgia. Bartram’s Bass are facing many risks, primary among them being hybridization with nonnative Alabama Bass. More recently, nonnative Alabama Bass and their hybrid congeners have been observed moving from reservoirs into tributaries where the remaining Bartram’s Bass populations exist. Thus, for Chapter 1 we sought to better understand the threat of nonnative gene spreading in tributary systems. To do this, we 1) quantified the movement of pure Alabama Bass, Bartram’s Bass, and their hybrids, 2) assessed correlations between movement and abiotic variables, …


Seasonal Variation In Condition, Body Lipid, Reproductive Investment, And Diet In Brook Stickleback (Culaea Inconstans), Matthew P. Basista, Randal Snyder, Christopher Pennuto, Alicia Perez-Fuentetaja May 2023

Seasonal Variation In Condition, Body Lipid, Reproductive Investment, And Diet In Brook Stickleback (Culaea Inconstans), Matthew P. Basista, Randal Snyder, Christopher Pennuto, Alicia Perez-Fuentetaja

Great Lakes Center Masters Theses

North-temperate fishes are subject to significant changes in abiotic and biotic conditions across seasons, which are likely reflected in temporal differences in energy dynamics, reproductive investment, and diet. This study explores seasonal changes in body lipid content, female reproductive investment (GSI), body condition (Fulton’s K), and diet in brook sticklebacks (Culaea inconstans) in Western New York. I expected body lipid content and body condition would decline during the breeding season reflecting energy expenditure for reproduction, and these parameters would increase in the summer and fall prior to the onset of winter. Based on previous studies, I also expected …


Diversification And Convergence Following The Transition From Saltwater To Freshwater In Stingrays., Autumn D. Magnuson May 2023

Diversification And Convergence Following The Transition From Saltwater To Freshwater In Stingrays., Autumn D. Magnuson

College of Arts & Sciences Senior Theses

One of the most fundamental questions in biology is why some groups of organisms are more diverse than others. Classic hypotheses for explaining differences in diversity consider distinctions in time, place, resources, and competitors as the staging grounds for differential diversification. Freshwater and saltwater environments have similar levels of diversity despite significant differences in size, so studying transitions between the two systems can provide insights into evolutionary processes. Despite the challenges associated with this transition, stingrays have invaded freshwater habitats multiple times across different continents, making them useful for better understanding these systems. In this study, I evaluated the frequency …


Unveiling The Ancestral Function Of A Neuroendocrine Regulator, Pou-I/Pit1: Insights From Gene Expression Analysis In The Sea Anemone Nematostella Vectensis, Miguel Agostinho Pina Da Silva May 2023

Unveiling The Ancestral Function Of A Neuroendocrine Regulator, Pou-I/Pit1: Insights From Gene Expression Analysis In The Sea Anemone Nematostella Vectensis, Miguel Agostinho Pina Da Silva

Graduate Theses and Dissertations

Cnidaria (i.e., sea anemones, jellyfish, corals) and Bilateria (i.e., vertebrates, sea stars, fruit flies), are sister groups that diverged around 600 million years ago. Despite the long evolutionary time, many cellular differentiation mechanisms, cell types, tissues and behaviors are conserved. Such as neurons, mechanosensory hair cells, feeding behaviors, peristaltic movements, and sleep. Recent advances in genomics, molecular biology and microscopy have fueled an increased interest in understanding cnidarian nervous and neuroendocrine systems. Understanding the developmental mechanisms and the mode of operation of Cnidarian nervous systems helps to reconstruct the ancestral nervous system of the last common ancestor of Cnidaria and …


Effects Of Maternal Disease History On Provisioning, Brooding, And Offspring Outcomes, Sakura Roberts May 2023

Effects Of Maternal Disease History On Provisioning, Brooding, And Offspring Outcomes, Sakura Roberts

Biological Sciences Undergraduate Honors Theses

Disease within a population has the ability to shape the development, evolution, and general performance of a species. Pathogen exposure to hosts can influence their physiology and behavioral patterns to further shape offspring immunity. Parental conditions experienced by offspring during early development can benefit survival and fitness (e.g. increasing provisioning rates), as well as help deter against similar diseases experienced by parents. By testing if parental behavior changes can better prepare offspring outcomes for disease exposure, such as disease severity or duration of infection, we can see the beneficial impacts it has on disease dynamics and host-pathogen processes. Incubation temperature, …


Exploring A Potential Bias In Detection Of Mesopredators By Cameras, Rylee Gibson May 2023

Exploring A Potential Bias In Detection Of Mesopredators By Cameras, Rylee Gibson

Biological Sciences Undergraduate Honors Theses

Mesopredators, such as the raccoon (Procyon locor), Virginia opossum (Didpelphis virginiana), and striped skunk (Mephitis mephitis) play crucial ecological roles as predators, prey, and disease vectors across much of the United States. Because of their importance and the way that populations of these mesopredators can dramatically increase due to human-subsidized resources, it is imperative that studies attempting to quantify mesopredator community composition are accurate and unbiased. However, it has recently been suggested that not all mammals trigger motion-activated wildlife game cameras at the same rate and for some species detection probability may be biased. My goals for this thesis were …


Past And Present Patterns Of Neutral And Adaptive Genetic Diversity In Wild Mandrills (Mandrillus Sphinx), Anna Weber May 2023

Past And Present Patterns Of Neutral And Adaptive Genetic Diversity In Wild Mandrills (Mandrillus Sphinx), Anna Weber

LSU New Orleans Theses and Dissertations

Although primates have fascinated researchers and the public alike for generations, one species that has remained enigmatic is the mandrill (Mandrillus sphinx), a large Cercopithecine monkey endemic to Central Africa. Mandrills are currently in decline due to bushmeat hunting, urbanization, and habitat loss. Neutral and adaptive genetic diversity are important tools for understanding evolutionary history and future viability, since diversity influences a species’ ability to adapt to a changing environment. However, thus far, minimal genetic information has been available for wild mandrills. Because of the dense vegetation in their tropical forest habitat, studying wild mandrills has proven to …


The Scaling Method: Body Mass Reconstruction Of East African Hominins, Julianna Rose May 2023

The Scaling Method: Body Mass Reconstruction Of East African Hominins, Julianna Rose

Anthropology Undergraduate Honors Theses

This thesis details a series of novel methods developed to estimate body masses of hominin fossils using 3-D point cloud registration software. All analyses were conducted through 3-D modeling software that supported the remote study of five fossil femora from East Africa. The fossil computer models were repeatedly aligned with anatomically modern human femora to determine their scaling relationship with the objective of using the scaling factor of the human references to estimate the body mass of the fossils, on the basis of the femoral head breadth. Body mass estimates obtained through this technique were compared to estimates using more …


Science, Community, And Culture: A Holistic Approach To Ecological Research And Education, Laura Whipple May 2023

Science, Community, And Culture: A Holistic Approach To Ecological Research And Education, Laura Whipple

All NMU Master's Theses

Global biodiversity has declined at an alarming rate over the past century as a result of many complex human-induced environmental changes. Standardized surveys have historically been used to identify drivers of species declines, but such studies are often resource-intensive, resulting in significant spatial and temporal data gaps when researchers lack the resources necessary to maintain such studies. One promising solution for overcoming gaps in standardized studies is the integration of species observations by community members (e.g., community science). Along with improving modeling techniques to address biodiversity declines, the education of future ecologists on the importance of Indigenous ecological knowledge, robust …


Patterns And Sources Of Variation In Heterospecific Pollen Deposition In Flowers Of The Native Blue Cardinal Flower (Lobelia Siphilitica), Allie Drinnon May 2023

Patterns And Sources Of Variation In Heterospecific Pollen Deposition In Flowers Of The Native Blue Cardinal Flower (Lobelia Siphilitica), Allie Drinnon

Undergraduate Honors Theses

Plants species interactions via pollinators are a model system to understand the mechanisms that generate plant diversity in nature. However, most studies have focused on plant-plant interactions via pollinator attraction while ignoring the role of plant-plant interactions via pollen transfer. Heterospecific pollen transfer (henceforth HP) can be common and have negative fitness effects. Negative HP fitness effects may prompt the evolution of adaptive strategies to minimize them. However, the extent of spatial variation in HP load size within and among populations, a tenet for natural selection, remains unexplored. Such knowledge would hence constitute a first step in advancing our understanding …


East Tennessee Spatial And Temporal Species-Specific Bat Activity Patterns, Seth Morelock May 2023

East Tennessee Spatial And Temporal Species-Specific Bat Activity Patterns, Seth Morelock

Undergraduate Honors Theses

Determining the activity among species-specific bat populations within specific habitat selections can help contribute to the conservation of Appalachian bat species. This study examines the differences in species-specific bat activity patterns between three ponds of variable sizes and a southeast-facing open field with a wooded edge. Four Song Meter SM4BAT FS bioacoustics detectors were used on a 15-acre property in Fall Branch, TN, with a wildlife acoustics detector being placed at three ponds and one field. There were three stages of data collection for this study which all took place during 2023. The first stage was during the spring (March …