Ecology and Evolutionary Biology Commons^™

Genome-Wide Changes In Genetic Diversity In A Population Of Myotis Lucifugus Affected By White-Nose Syndrome, Thomas M. Lilley, Ian W. Wilson, Kenneth A. Field, Deeann Reeder, Megan E. Vodzak, Gregory G. Turner, Allen Kurta, Anna S. Blomberg, Samantha Hoff, Carl J. Herzog, Brent J. Sewall, Steve Paterson

Faculty Journal Articles

Novel pathogens can cause massive declines in populations, and even extirpation of hosts. But disease can also act as a selective pressure on survivors, driving the evolution of resistance or tolerance. Bat white-nose syndrome (WNS) is a rapidly spreading wildlife disease in North America. The fungus causing the disease invades skin tissues of hibernating bats, resulting in disruption of hibernation behavior, premature energy depletion, and subsequent death. We used whole-genome sequencing to investigate changes in allele frequencies within a population of Myotis lucifugus in eastern North America to search for genetic resistance to WNS. Our results show low FST values …

Driver Of Avian Haemosporidian Diversity And Host Specificity In The Solomon Islands, Xena Marie Mapel

Biology ETDs

Avian haemosporidians are protist parasites that are distributed worldwide. We investigated these parasites in a tropical South Pacific archipelago to answer two questions: 1) Which community characteristics influence haemosporidian diversity; 2) Does host specificity vary across islands? To answer these questions we sequenced a mitochondrial gene and created linear models to establish relationships between select variables and diversity. In addition, we calculated host breadth of each lineage and constructed models to infer predictors of diversity. The percent of infected individuals on each island was correlated with island area. Haemosporidian phylogenetic diversity on a given island was associated with the proportion …

An Ecopath With Ecosim Analysis On Offshore Petroleum Platform Influences On Gulf Of Mexico Red Snapper, Valentin Gomez

LSU Master's Theses

Offshore oil and gas platforms have had a significant presence in the Gulf of Mexico since the 1950s. An important secondary function of these structures is that they provide artificial habitat to fisheries, most notably Red snapper. Policy changes intended to reduce the risk associated with aging infrastructure have reduced the number of standing platforms from 4044 to 1867 from 2001 to 2018. The effect this loss of habitat has on Red snapper was tested by creating three scenarios of platform changes and modeling the perturbation from 2005 to 2050. The simulation was accomplished using the ecological model Ecopath with …

Developing Tadpoles Exhibit Metabolic And Organ Size Plasticity In Competitive Rearing Environments, Emma Kimberly

Undergraduate Honors Thesis Projects

Abstract

Plasticity is the ability of an organism to respond to environmental variation by expressing different phenotypes. In Red-eyed treefrog tadpoles, Agalychnis callidryas, competitive environments induce long guts and short tails. Despite having a larger gut, tadpoles reared with competition do not increase intake when food becomes available. Pilot data suggest that this is because they have lower metabolic rates. The ability to maintain a larger gut with a depressed metabolic rate is confusing because guts are energetically expensive, and suggests that another energetic trade-off is taking place. The purpose of this study was to investigate the effect of …

Combined Opportunistic And Equilibrium Life-History Traits Facilitate Successful Invasions Of The Shimofuri Goby (Tridentiger Bifasciatus), Jiao Qin, Miao Xiang, Meixiang Jia, Fei Cheng, Bjorn V. Schmidt, Jian Liu, Songguang Xie

Faculty Publications

The life-history traits of a non-native population of Shimofuri goby (Tridentiger bifasciatus) were investigated in Nansi Lake, which is a storage lake on the East Route of the South-to-North Water Transfer Project, China. The results were analyzed in combination with data from other non-native populations of this species to reveal the life-history traits that caused its successful invasions. Mature gonads in April and May suggest that the species spawns in this period, and cohort tracking and age analysis revealed that individuals had a one-year lifespan. The relative fecundity was 2,825 ± 614 eggs/g. A short lifespan and high fecundity are …

Fungicide Sensitivity Of Sclerotinia Sclerotiorum Isolates Selected From Five Different States That Use Different Fungicide Treatments, Cristian Wulkop Gil

UCARE: Research Products

Sclerotinia sclerotiorum is a plant pathogenic fungus that causes a disease called white mold that can infect more than 450 plant species including soybeans, dry beans, green beans, canola, and sunflower. This pathogen is capable of up to $252M in losses every year (U.S. Canola Association, 2014). Fungicides are widely used in developed agricultural systems to control disease. However, resistance to the most effective fungicides has emerged and spread in pathogen populations and there have been multiple reports of S. sclerotiorum isolates becoming resistant to certain fungicides. Since different fields in different states use different fungicide treatments on plants and …

Relative Model Fit Does Not Predict Topological Accuracy In Single-Gene Protein Phylogenetics, Stephanie J. Spielman

College of Science & Mathematics Departmental Research

It is regarded as best practice in phylogenetic reconstruction to perform relative model selection to determine an appropriate evolutionary model for the data. This procedure ranks a set of candidate models according to their goodness of fit to the data, commonly using an information theoretic criterion. Users then specify the best-ranking model for inference. Although it is often assumed that better-fitting models translate to increase accuracy, recent studies have shown that the specific model employed may not substantially affect inferences. We examine whether there is a systematic relationship between relative model fit and topological inference accuracy in protein phylogenetics, using …

Scorpions Of The Horn Of Africa (Arachnida: Scorpiones). Part Xxiii. Buthus (Buthidae), With Description Of Two New Species, František Kovařík, František Šťáhlavský, Hassan S. A. Elmi

Euscorpius

New data are presented on the distribution of the genus Buthus Leach, 1815 in the Horn of Africa, mainly in Somaliland, acquired during expeditions in 2011–2019. Buthus berberensis Pocock, 1900, for which the exact locality was not known, was collected again. B. zeylensis Pocock, 1900 is restored from synonymy and elevated to species rank, based on a study of 75 recently collected specimens. Two new species, B. pococki sp. n. and B. somalilandus sp. n., are described, fully complemented with color photographs of live and preserved specimens, as well as their habitats. In addition to the analyses of external …

Scorpion Predation In Cuba: New Cases And A Review, Tomás M. Rodríguez-Cabrera,, Rolando Teruel, Ernesto Morell Savall

Euscorpius

The ecology of Cuban scorpions is very insufficiently studied and the scarce existing information on their natural enemies is dispersed in the literature. However, scorpions in general are well known to play an important role both as predators and prey in natural ecosystems. Herein we present new instances of predation on different species of scorpions in Cuba, and a review on the topic

Variation In The Microbiota Associated With Daphnia Magna Across Genotypes, Populations, And Temperature, Jonas Frankel-Bricker, Michael J. Song, Maia J. Benner, Sarah Schaack

University Author Recognition Bibliography: 2020

Studies of how the microbiome varies among individuals, populations, and abiotic conditions are critical for understanding this key component of an organism’s biology and ecology. In the case of Daphnia, aquatic microcrustaceans widely used in population/community ecology and environmental science studies, understanding factors that influence microbiome shifts among individuals is useful for both basic and applied research contexts. In this study, we assess differences in the microbiome among genotypes of D. magna collected from three regions along a large latitudinal gradient (Finland, Germany, and Israel). After being reared in the lab for many years, we sought to characterize any …

Nine-Mile Prairie Environs: Master Plan, April 2020, Center For Grassland Studies

Center for Grassland Studies: Newsletters

A red and white checkered water tower stands atop the hills on Lincoln’s northwest fringe. The tower sustains water pressure and is a waypoint finder for air traffic approaching the Lincoln airfield. In addition to these services, the tower’s checker pattern can symbolize the surrounding patchwork of native unplowed tallgrass prairie that comprises the Nine-Mile Prairie (NMP) Environs. At the core is NMP, a 230-acre public property full of tallgrass prairie biodiversity and Nebraska history. An area surrounding NMP, referred to as the “Environs” in the context of this strategic planning document, is almost entirely grassland or agricultural cropland. The …

Artificial Perches As A Technique For Enhancing Tropical Forest Restoration: A Case Study From The Dominican Republic, Spencer Schubert, Ally S. Lahey, Ashley R. Weisman, Eric L. Walters

College of Sciences Posters

Recovering secondary forests on degraded agricultural lands represents a promising opportunity to offset global carbon emissions as well as increasing local biodiversity and ecosystem services. In the insular tropical forests of the Caribbean, frugivorous birds are the primary seed dispersers for most native woody plants and have a large influence on regeneration dynamics during forest succession. In 2017, we initiated an experimental forest restoration program incorporating artificial perches on private farms within the Rio Yaque del Norte watershed in La Vega province, Dominican Republic. Five restoration plots (0.15–0.25 ha) were constructed in pastures near deforested streams. In each plot, 6-12 …

Effects Of Stream Permanence On The Stoneflies (Plecoptera) Of The Mammoth Cave Region, Taylor Mcroberts

Masters Theses & Specialist Projects

Stoneflies (Plecoptera) are often associated with cold perennial streams, but many species also inhabit intermittent streams that experience reduced or lack of flow during summer and autumn. In this study the influence of stream permanence on stonefly assemblage composition and spatial distribution in the Mammoth Cave region, Kentucky, USA, was addressed based on a 14 month sampling regime from the fullest range of stream sizes and habitable flow regions available. Adult stoneflies were collected monthly at Mammoth Cave National Park and the Western Kentucky University Green River Preserve. Collections were done from December 2018–October 2019 using a standard timed protocol …

Nutrient Removal Potential Of Constructed Wetlands In Grand Lake St. Marys Watershed, Stephen J. Jacquemin, Jocelyn Birt, Benjamin Strang, Conner Ewing, Bradley Axe, T. Dirksen

Lake Campus Research Symposium Abstracts and Posters

Constructed wetlands are becoming an increasingly important management tool to reduce nutrient rich agricultural runoff in the Great Lakes region. The objective of this study was to assess the removal efficiency of two constructed wetlands operating on tributaries of Grand Lake St. Marys (Prairie Creek and Coldwater Creek) located in northwest Ohio. Water samples were collected weekly during 2019 year from inflow and outflow points where they were analyzed for nutrient (nitrate-N, total phosphorus, dissolved reactive phosphorus) concentrations following standard EPA colorimetric methods. Overall, while both wetlands experienced high mean nutrient inputs (concentrations in mg/L) across both fall and summer …

Continuous Water Quality Monitoring Platform For Grand Lake St Marys, Aaron Neikamp, Alex Lehman, Brandon Siefring, Jason Evers, Ryan M. Spicer, Shayna R. Petitjean

Lake Campus Research Symposium Abstracts and Posters

For the past decade, Grand Lake St. Marys (GLSM) has struggled to provide a stable and clean water source for the community affecting people and businesses alike. A safe level of microcystin –a toxin in the harmful algal blooms–is 20 ppb in recreational water, and GLSM has seen an excess of 82 ppb. As of now, there is no solution to continuously monitor the water quality; therefore, corrective actions are only based off intermittent samples taken by hand. A solution to this issue would be a water quality platform (WQP) that monitors parameters such as water and air temperature, conductivity, …

Substrate-Borne Vibrational Communication In Veiled Chameleons (Chamaeleo Calyptratus) During Courtship, Breeding, And Territoriality, Lauren Kappel

Masters Theses & Specialist Projects

Substrate-borne vibrations, or biotremors, are utilized by vertebrates found in unique environments because biotremors are an effective way to transmit signals through dense media. Previous studies have shown that veiled chameleons (Chamaeleo calyptratus) are able to produce biotremors via specialized neck muscles. I hypothesized that during courtship and/or breeding, the veiled chameleon, a tree-dwelling species, would communicate with biotremors through branches. Additionally, I hypothesized that female call characteristics would differ between reproductive condition (i.e., receptive and non-receptive), while male call characteristics would differ between behavioral contexts (i.e., territorial vs. courtship). Chameleons were paired (one male, one female) and placed on …

Potential Of Pasture Grasses To Reduce Soil Runoff In Simulated Spring Seeding Applications, Aubrey Jaqueth, Stephen J. Jacquemin

Lake Campus Research Symposium Abstracts and Posters

Declines in surface water quality has emerged as one of the foremost environmental, social, and political issues in the Midwestern United States over the past several decades. One of the leading causes of water quality issues in this region has been linked to non-point source surface runoff of soil, nutrients, and chemicals from primarily agricultural landscapes. Surface runoff negatively affects water by facilitating eutrophic conditions and additionally, negatively impacts landscapes by reducing the organic and top soil layers leading to production declines. Thus, best management practices that focus on reducing runoff rates in agricultural acreage are a high priority. The …

Delineation Of Undescribed, Morphologically Cryptic Cave Beetles Of The Pseudanophthalmus Pubescens Species-Group (Coleoptera: Carabidae: Trechinae), Jedidiah John Nixon

Masters Theses & Specialist Projects

The genus Pseudanophthalmus Jeannel is a widespread and extremely diverse taxon of troglobiont beetle endemic to the karst of eastern North America, with its distribution centered in Tennessee and Kentucky. Despite lying near the heart of this region, the pubescens species-group was thought to contain many undescribed species. In this study, the validities of several of the late Dr. Thomas Barr’s unpublished putative new species in the pubescens-group were tested both morphologically and molecularly. Body measurements (length and width for head, thorax, and abdomen), as well as male genital morphology were compared to see if they reflected theorized species limits.. …

An Intergrative Ecological And Taxonomic Assessment Of The Stoneflies (Insecta, Plecoptera) Of The Black Mountains, North Carolina, Usa, Madeline Lake Metzger

Masters Theses & Specialist Projects

The Black Mountains are a part of the Blue Ridge region of the Appalachian Mountains and include Mount Mitchell, the highest peak east of the Mississippi River at 2,037 m (= 6,684 ft). A comprehensive inventory of the stonefly fauna was conducted between 2014-2019 within Mt. Mitchell State Park and adjacent Pisgah National Forest. Collections occurred seasonally from 44 unique locations, covering a broad range of stream sizes and elevations in the region. Sequencing of the mitochondrial cytochrome c oxidase subunit I (mtCOI) gene was completed to assist with making positive determinations to species for females of taxonomically challenging genera …

Can Protozoa Prove The Beginning Of The World?, Karina L. Burton

Classical Conversations

Protozoa are magnificent creatures. They exhibit all of the functions intrinsic to living organisms: irritability, metabolism, growth and reproduction. Within these functions, there are numerous examples of mutations that occur in order for organisms to adapt to their given environments. Irritability is demonstrated in protozoa by their use of pseudopodia, flagella, or cilia for motility; it has been shown that such locomotors exhibit diversity while maintaining similar protein and chemical structures that appear to be a result of evolutionary processes. Metabolism in protozoa is similar to that of larger animals, but their diet is unique. They primarily feast upon bacteria, …

Lightning Damage Stimulates Beetle Activity In A Tropical Forest, Samantha C. Vaughn, Kane A. Lawhorn, Evan M. Gora, Steve P. Yanoviak

Undergraduate Arts and Research Showcase

Disturbance alters the structure of ecological communities. Localized disturbances in tropical rainforests often create canopy gaps - patches of forest where large trees have fallen or are defoliated. Lightning is a major cause of large-tree mortality, and consequently gaps, in tropical forests. Lightning-caused gaps consist of abundant dead standing wood which likely is a predictable resource for saproxylic arthropods, specifically wood-boring beetles (Coleoptera). The goal of this study was to provide a preliminary evaluation of the beetles that are attracted to lightning-damaged trees in a tropical forest. We placed flight intercept traps in the subcanopy of 8 trees (4 struck …

Distortion Of The Local Magnetic Field Appears To Neither Disrupt Nocturnal Navigation Nor Cue Shelter Recognition In The Amblypygid Paraphrynus Laevifrons, Daniel D. Wiegmann, Patrick Casto, Eileen Hebets, Verner P. Bingman

Eileen Hebets Publications

Many arthropods are known to be sensitive to the geomagnetic field and exploit the field to solve spatial problems. The polarity of the geomagnetic field is used, for instance, as an orientation cue by leafcutter ants as they travel on engineered trails in a rainforest and by Drosophila larvae as they move short distances in search of food. A ubiquitous orientation cue like the geomagnetic field may be especially useful in complex, cluttered environments like rainforests, where the reliability of celestial cues used to navigate in more open environments may be poor. The neotropical amblypygid Paraphrynus laevifrons is a nocturnal …

A Phenotypic Comparison Of Regional Populations Of Hemileuca Maia (Drury, 1773) With Designations Of New Subspecies (Bombycoidea, Saturniidae, Hemileucinae), Harry Pavulaan

The Taxonomic Report of the International Lepidoptera Survey

Following refinement of the type locality of Hemileuca maia to the Long Island Pine Barrens of New York State by the author (Pavulaan, 2020), an evaluation of phenotypic characters of regional populations of H. maia is presented. The Long Island population is the nominotypical subspecies. Populations in southeastern coastal New England and offshore islands are presently considered nominotypical maia. However, several continental inland populations show evidence of subspecific variation. Four new subspecies are designated. Detailed phenotypic information of other interior regions is lacking.

Diversity Of The Major Histocompatibility Complex In African Penguins (Spheniscus Demersus) In Situ, Athena Schalk

Undergraduate Honors Thesis Projects

The Major Histocompatibility Complex (MHC) is responsible for the immune response in all jawed vertebrates and protects individuals against a variety of pathogens and diseases. Maintaining genetic diversity within the MHC exons is critical to protecting endangered species. African penguins (Spheniscus demersus) are in danger of losing their MHC diversity in isolated populations due to reductions in population size associated with environmental changes and human activity. This study analyzes the diversity within the exons in the DNA encoding the MHC by amplifying the exons through polymerase chain reaction and identifying alleles through denaturing gradient gel electrophoresis. Wild populations of …

Assessing Microplastic Pollution In Four Mile Run, An Urban Stream In Northern Virginia, Kurt Moser, Tessa Naughton-Rockwell, Louisa Wang, Ethan Litmans, Amanda Manoogian

Virginia Journal of Science

Microplastics are pollutants of concern in waterways and oceans for their persistence and impact on aquatic life and food webs. This study demonstrates a low-cost land-based sampling method to assess the extent of microplastic pollution found in Four Mile Run, an urban stream in Northern Virginia. Microplastic particle counts in environmental and treated wastewater ranged from 0.01-0.24 particles L^-1 (mean 0.08 particles L^-1) and from 2 to 446 µg L^-1 (mean 70 µg L^-1), with fibers found to be the most common microplastic category. Treated wastewater effluent was found to be a significant source …

Physiological And Behavioral Effects Of Climate Change On Wildlife: An Introduction And Overview, Andy Clarke

Senior Honors Theses

Planetary environmental system changes have been recorded and documented for several decades. Fluctuations that were first noticed in atmospheric carbon-dioxide levels have now extended into global pattern changes. Climatic variations that were initially non-threatening variabilities have since been observed creating significant biological influences. The results and evidence of the effects of worldwide environmental climate change on wildlife and biotic environments are worth examining because of the impacts it has on the planet. These climatic effects extend from changes in distribution and diversity patterns of terrestrial mammals to sea-life impacts and recovery trends. Possible wildlife benefits may include increased humidity and …

West Coast Rock Lobster Whale Entanglement Mitigation Workshop, Jason How, L Orme, Graeme Baudains, Pia Dobson, M Taylor, Simon De Lestang

Fisheries Occasional Publications

No abstract provided.

The Impact Of Nicotine Accumulation Exposure On Lithobates Catebeianus Larvae Mortality, Luke Micek

UCARE: Research Products

Cigarette butts are the most littered item in the world and contain over 4,000 chemicals, including the organic compound nicotine (Slaughter et al. 2011). Billions of cigarette butts are littered each year which may wash into bodies of water, impacting the local wildlife. To determine how introduced chemicals impact the environment, it is important to study its effects on indicator species. Amphibians, such as Lithobates catebeianus, act as indicator species due to their extreme sensitivity to chemical changes in its environment. The purpose of this research project was to obtain data to help determine the impact nicotine accumulation has …

Spider Prey Of Mud-Dauber Wasps In Southeastern Nebraska, Earl Agpawa, Tyler Corey, Eileen Hebets

UCARE: Research Products

Predator-prey interactions between organisms provide a window into behaviors, adaptions, and evolutionary histories of both groups. One group of organisms of would be female spider-specialist mud-dauber wasps (Hymenoptera: Sphecidae) who seek out and sting spiders to bring back to their nests for their larvae to feed upon. This interaction provides a unique means of quantifying the diversity and abundance of the prey they capture. In this study, we inventoried spiders from within collected nests of mud-dauber wasps Sceliphron caementarium (Drury) and Chalybion californicum (Saussure) from agricultural land and in a forest corridor. It was found that nests collected in agricultural …

Using Δ18o To Track Po4 Entering The Western Basin Of Lake Erie, Melanie M. Marshall, Gabrielle K. Metzner, Kevin E. Mccluney

Lake Campus Research Symposium Abstracts and Posters

Algal blooms in the Western Basin of Lake Erie are dependent upon nutrients provided by major rivers within Northwest Ohio. To develop more accurate methods of defining which of these waterways is the largest contributor, a proof of concept study is being conducted using δ18O of phosphate molecules. In the summer of 2016, under relatively low stream flow conditions, 10-20L samples of water were collected at the several major branches within the Portage River, at the mouths of the Portage, Maumee, and Sandusky Rivers, and at two locations within the Western Basin. In the spring of 2017, these collections were …