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Articles 661 - 690 of 777
Full-Text Articles in Ecology and Evolutionary Biology
Comparison Of Isotope-Based Biomass Pathways With Groundfish Community Structure In The Eastern Gulf Of Mexico, Sheri Ann Huelster
Comparison Of Isotope-Based Biomass Pathways With Groundfish Community Structure In The Eastern Gulf Of Mexico, Sheri Ann Huelster
USF Tampa Graduate Theses and Dissertations
This study compared traditional community analysis with stable-isotope trophic analysis to define process-based trophic elements of community structure in the eastern Gulf of Mexico, and developed a predictive capability regarding changes to fish community structure that would be expected from increasing eutrophication. Specifically, it used an existing trawl survey program (SEAMAP) to compare invertebrate herbivore (sponge and sea urchin) isotopes with groundfish isotopes, and then compared the resulting spatial patterns with spatial variation in community structure, as identified by cluster analysis. The comparison was applied to seven NMFS survey zones that extended offshore from the Caloosahatchee River, FL northwest to …
Historical And Current Population Patterns Of The Staghorn Coral (_Acropora Cervicornis_) In Dry Tortugas National Park, Kaitlyn Lizza
Historical And Current Population Patterns Of The Staghorn Coral (_Acropora Cervicornis_) In Dry Tortugas National Park, Kaitlyn Lizza
USF Tampa Graduate Theses and Dissertations
Acropora cervicornis was once one of the dominant reef building corals of the Caribbean, Florida Keys, and Dry Tortugas (DRTO), but since the 1970’s populations have been decimated throughout their geographic range. Recently, a repopulation was documented through detailed benthic surveys conducted by the U.S. Geological Survey at three locations (Pulaski Shoal, East Key, and Loggerhead Key) within DRTO. Benthic surveys using the U.S Geological Survey’s Along-Track Reef Imaging System (ATRIS) revealed hundreds of previously undocumented colonies.
These discoveries have provided a unique data-set, allowing a comparison between the historical (1883, 1976) and contemporary distributions (2009, 2011) of A. cervicornis. …
Comparison Of Gastropod Assemblages From Natural And Phosphate Mine Lakes Of Central Florida, William A. Mailand
Comparison Of Gastropod Assemblages From Natural And Phosphate Mine Lakes Of Central Florida, William A. Mailand
USF Tampa Graduate Theses and Dissertations
Investigations were made examining the relationships between gastropod species richness and abundance across 20 phosphate and 20 natural lakes in Central Florida. In additional to lake category, age of phosphate lakes was used to determine if phosphate lakes ever approximate natural lakes. Additional physical, chemical, and biological parameters, including chlorophyll a, Ca, secchi, phosphorous, conductance, fish predation, and recreational lake use were investigated in order to determine if they affected gastropods with lake age. Comparisons were also made between gastropod species richness and average abundance and two groups of dominant vegetation categories: Panicum, a structurally complex macrophyte, and Typha …
Impacts Of Agrochemical Pollution On Aquatic Communities And Human Disease, Neal T. Halstead
Impacts Of Agrochemical Pollution On Aquatic Communities And Human Disease, Neal T. Halstead
USF Tampa Graduate Theses and Dissertations
The global human population is expected to exceed 9 billion individuals by 2050, putting greater strain on the natural resources needed to sustain such a population. To feed this many people, some expect agricultural production will have to double and agrochemical use will have to increase anywhere from two- to five-fold relative to the turn of the century. Although industrial agriculture has provided many benefits to society, it has caused declines in biodiversity, both directly (e.g., through conversion of habitat) and indirectly (e.g., through contamination of adjacent natural habitats). Agricultural activity has also been linked to increased prevalence and intensity …
A Basic Guide For Empirical Environmental Social Science, Michael Cox
A Basic Guide For Empirical Environmental Social Science, Michael Cox
Dartmouth Scholarship
In this paper, I address a gap in the literature on environmental social science by providing a basic rubric for the conduct of empirical research in this interdisciplinary field. Current literature displays a healthy diversity of methods and techniques, but this has also been accompanied by a lack of consistency in the way in which research in this area is done. In part this can be seen as resulting from a lack in supporting texts that would provide a basis for this consistency. Although relevant methods texts do exist, these are not written with this type of research explicitly in …
A Diagnostic Procedure For Applying The Social-Ecological Systems Framework In Diverse Cases, Jochen Hinkel, Michael E. Cox, Maja Schlufffdter, Claudia R. Binder
A Diagnostic Procedure For Applying The Social-Ecological Systems Framework In Diverse Cases, Jochen Hinkel, Michael E. Cox, Maja Schlufffdter, Claudia R. Binder
Dartmouth Scholarship
The framework for analyzing sustainability of social-ecological systems (SES) framework of Elinor Ostrom is a multitier collection of concepts and variables that have proven to be relevant for understanding outcomes in diverse SES. The first tier of this framework includes the concepts resource system (RS) and resource units (RU), which are then further characterized through lower tier variables such as clarity of system boundaries and mobility. The long-term goal of framework development is to derive conclusions about which combinations of variables explain outcomes across diverse types of SES. This will only be possible if the concepts and variables of the …
Why Do Caterpillars Whistle? Acoustic Mimicry Of Bird Alarm Calls In The Amorpha Juglandis Caterpillar, Jessica Lindsay
Why Do Caterpillars Whistle? Acoustic Mimicry Of Bird Alarm Calls In The Amorpha Juglandis Caterpillar, Jessica Lindsay
Undergraduate Theses, Professional Papers, and Capstone Artifacts
Caterpillar species possess a range of anti-predator defenses, from regurgitation to sonar jamming. The North American walnut sphinx caterpillar (Amorpha juglandis) produces a variety of whistling noises when pinched. Limited observations indicate that this causes avian predators to retreat, leaving the caterpillar alone. However, it is unknown why this whistle is such an effective deterrent. Interestingly, the A. juglandis whistle is acoustically similar to the “seet” alarm call that many bird species produce in response to their own predators. We propose that the A. juglandis whistle is a form of acoustic mimicry, in which the caterpillar protects itself …
Investigating The Role Of Polyploidy In The Response Of Chamerion Angustifolium To Increased Soil Nitrogen Availability And Insect Herbivory, Alex Bales
Dissertations, Master's Theses and Master's Reports
Human activities have more than doubled the input rate of nitrogen to terrestrial ecosystems. Increased soil nitrogen availability is associated with increased plant productivity, changes in plant resource allocation strategies, and changes in interactions between plants and their associated insect herbivores. For example, nitrogen-rich conditions can alter the physiological and chemical properties of plants in ways that increase their ability to deter herbivore attack (resistance) and maintain fitness following herbivore attack (tolerance). The ecological tolerance of plants to such abiotic and biotic environments is thought to be influenced by autopolyploidy, a commonly occurring phenomenon in plants in which individuals have …
Investigating Within-Canopy Variation Of Functional Traits And Cellular Structure Of Sugar Maple (Acer Saccharum) Leaves, Adam P. Coble
Investigating Within-Canopy Variation Of Functional Traits And Cellular Structure Of Sugar Maple (Acer Saccharum) Leaves, Adam P. Coble
Dissertations, Master's Theses and Master's Reports - Open
Patterns of increasing leaf mass per area (LMA), area-based leaf nitrogen (Narea), and carbon isotope composition (δ13C) with increasing height in the canopy have been attributed to light gradients or hydraulic limitation in tall trees. Theoretical optimal distributions of LMA and Narea that scale with light maximize canopy photosynthesis; however, sub-optimal distributions are often observed due to hydraulic constraints on leaf development. Using observational, experimental, and modeling approaches, we investigated the response of leaf functional traits (LMA, density, thickness, and leaf nitrogen), leaf carbon isotope composition (δ13C), and cellular structure to light availability, height, and leaf water …
Translocation And Telemetry Tracking Of Lake Sturgeon In The Menominee River, Mi / Wi, Jeremy G. Olach
Translocation And Telemetry Tracking Of Lake Sturgeon In The Menominee River, Mi / Wi, Jeremy G. Olach
Dissertations, Master's Theses and Master's Reports - Open
The Menominee River is a Michigan-Wisconsin boundary water historically traversed by lake sturgeon (Acipenser fulvescens), but now contains fragmented populations between hydroelectric dams. Though fish passage is currently being implemented on the lower dams, it is unclear whether sturgeon given access to historic spawning grounds would use them. In 2012 and 2013, a total of 15 pre-spawning sturgeon were captured, implanted with sonic transmitters and translocated upstream over two hydroelectric dams to the stretch below the historic spawning site of Sturgeon Falls. Sturgeon were then tracked via five stationary receivers from April 2012 until August 2013, and with …
Biogeochemical Cycling In Lake Superior Tributaries: Seasonality, Quantity And Quality Of Export, Ashley Anne Coble
Biogeochemical Cycling In Lake Superior Tributaries: Seasonality, Quantity And Quality Of Export, Ashley Anne Coble
Dissertations, Master's Theses and Master's Reports - Open
Seasonal and spatial variability in environmental factors may affect dissolved organic matter composition and nutrient transformation and retention in streams. The objective of this research was to quantify and describe seasonality, quantity, and quality of nutrient processing and export of ammonium (NH4), soluble reactive phosphate (SRP), and dissolved organic carbon (DOC) into Lake Superior through intensive study in a small 1st order watershed coupled with snapshot measurements across 12 tributaries that varied in size, location, and wetland coverage. Our results suggest biodegradable C is exported from a small headwater stream year-round and that DOC mineralization rates can be …
Oviposition Site Selection By Mosquitoes: Predator-Released Kairomones And Responses To Different Taxa, Lauren Larea Eveland
Oviposition Site Selection By Mosquitoes: Predator-Released Kairomones And Responses To Different Taxa, Lauren Larea Eveland
Electronic Theses and Dissertations
Oviposition site selection is a critical reproductive behavior for egg-laying organisms that offer little to no parental care. Determining the mechanisms females use to assess sites is critical to understanding life history traits, community structure, and species interactions. Mosquitoes are excellent model organisms for studying oviposition site selection for numerous reasons: 1) a poor decision can lead to zero reproductive success, 2) females actively assess and choose among habitat patches when locating an oviposition site, 3) larvae remain in the habitat where they hatch until they metamorphose, and 4) they lay conspicuous egg rafts that allow direct assessment of oviposition. …
Assessing The Permeability Of Landscape Features To Animal Movement: Using Genetic Structure To Infer Functional Connectivity, Sarah J. Anderson, Elizabeth M. Kierepka, Robert K. Swihart, Emily K. Latch
Assessing The Permeability Of Landscape Features To Animal Movement: Using Genetic Structure To Infer Functional Connectivity, Sarah J. Anderson, Elizabeth M. Kierepka, Robert K. Swihart, Emily K. Latch
Department of Forestry & Natural Resources Faculty Publications
Human-altered environments often challenge native species with a complex spatial distribution of resources. Hostile landscape features can inhibit animal movement (i.e., genetic exchange), while other landscape attributes facilitate gene flow. The genetic attributes of organisms inhabiting such complex environments can reveal the legacy of their movements through the landscape. Thus, by evaluating landscape attributes within the context of genetic connectivity of organisms within the landscape, we can elucidate how a species has coped with the enhanced complexity of human altered environments. In this research, we utilized genetic data from eastern chipmunks (Tamias striatus ) in conjunction with spatially explicit habitat …
Ecology/ Graduate Students/ Westra & Walton/ University Of Oregon/ 2012, Brian Westra, Dean Walton
Ecology/ Graduate Students/ Westra & Walton/ University Of Oregon/ 2012, Brian Westra, Dean Walton
Data Information Literacy Case Study Directory
The case study covers a one-shot seminar style Data Information Literacy program offered to graduate students associated with an NSF funded Ecology project. Topics covered included file formats, publishing data, metadata, preservation and data citation. Materials include a book chapter describing the case study, the lesson plan for the session and the evaluation form used to gather feedback.
Unraveling The Genetic Mechanisms Involved In The Evolution And Development Of The Thoracic Appendages In Insects, Victor Medved
Unraveling The Genetic Mechanisms Involved In The Evolution And Development Of The Thoracic Appendages In Insects, Victor Medved
Wayne State University Dissertations
Insects display the greatest amount of structural and functional variation among animal groups, particularly in regard to their appendage morphology. These differences can range from the diverse pigmentation patterns between fore- and hindwings to changes in the size and shape of legs. The greatly enlarged jumping hind leg in crickets and grasshoppers is one of the best known illustrations of such diversity, representing a unique feature for the entire order of these insects (Orthoptera). Previous work from our lab has shown that the homeotic gene Ultrabithorax (Ubx) plays a key role in the enlargement of hind legs not only in …
Habitat Value Of Restored Intertidal Shoreline For Fish And Macrobenthic Communities In Northeast Florida, Shannon K. Dunnigan
Habitat Value Of Restored Intertidal Shoreline For Fish And Macrobenthic Communities In Northeast Florida, Shannon K. Dunnigan
UNF Graduate Theses and Dissertations
Oyster reefs are declining worldwide, as well as the economic and ecological value of oysters to their respective systems. Numerous restoration efforts have been undertaken in hopes of re-establishing these shellfish populations. This study evaluated a restoration project within the Guana Tolomato Matanzas estuary in northeast Florida, U.S.A., to investigate community structure as well as seasonal patterns in species abundance and diversity of juvenile fish and benthic macrofauna within restored and unrestored intertidal habitats along the Guana Peninsula. The first objective was to determine whether the artificially created reefs provided similar quantity and diversity of benthic macrofauna as adjacent unrestored …
Reproductive Biology Of Syntopic Blackside Dace And Southern Redbelly Dace In Two Kentucky Streams, Brajaan Hayes
Reproductive Biology Of Syntopic Blackside Dace And Southern Redbelly Dace In Two Kentucky Streams, Brajaan Hayes
Online Theses and Dissertations
When a rare endemic species and an ecologically similar, but more cosmopolitan species share habitat, understanding how they interact with each other is important for conservation efforts. This is especially true if both species rely on the same spawning habitat, which could potentially lead to competitive displacement or hybridization. It is known that the federally threatened Blackside Dace and common Southern Redbelly Dace are ecologically similar species, occur syntopically in Kentucky in the upper Cumberland River basin, and utilize some of the same host species for nest sites; however, it is unclear if the two fish species are spawning site …
Effects Of Supplemental Food On The Behavior, Aggression, And Paternity Status Of Male Indigo Buntings (Passerina Cyanea), Bradley Mcleod
Effects Of Supplemental Food On The Behavior, Aggression, And Paternity Status Of Male Indigo Buntings (Passerina Cyanea), Bradley Mcleod
Online Theses and Dissertations
Breeding is an energetically costly activity for birds. If energy-limited, birds may alter their time budgets, reducing time spent in some activities and spending more time in others. To date, no experimental study has investigated the possible effect of food availability on male mate guarding behavior. Additionally, previous results from food supplementation studies are mixed. My objectives were to determine how food supplementation might influence the breeding behavior of male Indigo Buntings (Passerina cyanea). I predicted that, compared to non-supplemented males, food-supplemented males would: 1) spend less time foraging, 2) spend more time singing, 3) spend more time mate guarding, …
Flight Vocalizations And Displays Of Adult Chimney Swifts (Chaetura Pelagica): Description And Function, Louise Ann Peppe
Flight Vocalizations And Displays Of Adult Chimney Swifts (Chaetura Pelagica): Description And Function, Louise Ann Peppe
Online Theses and Dissertations
The vocalizations of many songbirds have been well documented and analyzed, but less is known about the vocal behavior of many non-passerines, including swifts. While flying alone as well as during aerial displays with conspecifics, Chimney Swifts (Chaetura pelagica) often utter a twitter call consisting of a series of high-frequency chip notes. However, little is known about the possible function(s) of swift flight displays and their twitter call. My objectives were to record, analyze, and document the aerial behaviors and associated vocalizations of adult Chimney Swifts. I studied swifts at the Blue Grass Army Depot in Madison County, Kentucky, where …
Fate Of Captive-Reared Bobwhite Quail Released In Central Kentucky, Adam Carter
Fate Of Captive-Reared Bobwhite Quail Released In Central Kentucky, Adam Carter
Online Theses and Dissertations
The fate of pen-raised bobwhite quail (Colinus virginianus) released onto two farms (Site 1 and 2) and a reclaimed coal surface mine (Site 3) in south-central Kentucky during the summers of 2008 and 2009 was assessed. Of the 57 bobwhite quail radio-tagged and released in this study, the fate of 26 (45%) was unknown because the telemetry signal was lost and the birds could not be located. Within 34 days after release, mortality of the remaining 31 radio-tagged birds was 100%. Raptors, coyotes, and mesomammal predators were responsible for most (84%) of the identified quail mortality; accounting for the deaths …
Offspring Retrieval Behaviors After Brood Loss In The Convict Cichlid, Heather Witherspoon Govert
Offspring Retrieval Behaviors After Brood Loss In The Convict Cichlid, Heather Witherspoon Govert
Online Theses and Dissertations
Parents provide care for their young in several different ways. One of these is searching for and retrieving any young that may become displaced from their nest. In monogamous biparental species, parents may share care of their young, including retrieval efforts. Convict cichlids (Amatitlania siquia) are an example of a monogamous biparental fish that show shared parental care of young. In the wild, these cichlids defend nests and search for missing young in response to complete or catastrophic brood loss. This retrieval behavior is important for both parents because parents, especially the males, have been observed to abandon their mate …
Vegetation-Based Metrics Of Biotic Integrity For Assessing The Ecological Condition Of Wetlands Of Kentucky, Tanner Matthew Morris
Vegetation-Based Metrics Of Biotic Integrity For Assessing The Ecological Condition Of Wetlands Of Kentucky, Tanner Matthew Morris
Online Theses and Dissertations
Over the last two centuries, wetland acreage across the world has significantly declined due to human disturbances. It has been estimated that Kentucky has lost over 80% of its wetland area. In response to these losses occurring across the United States, the Clean Water Act was passed to halt this dramatic decline and to restore the ecological integrity of waters of the United States. To enforce the Clean Water Act, a number of ecological assessment techniques have been developed to quantify the ecological quality of the waters of the United States. Kentucky recently adopted a rapid method for assessing the …
Validating A Kentucky Wetland Rapid Assessment Method For Forested Riverine Wetlands Using Vegetation, Bird Surveys, And Landscape Analysis, Johnryan Andrew Polascik
Validating A Kentucky Wetland Rapid Assessment Method For Forested Riverine Wetlands Using Vegetation, Bird Surveys, And Landscape Analysis, Johnryan Andrew Polascik
Online Theses and Dissertations
Within the last two centuries, Kentucky has undergone wetland losses exceeding 80 percent (approximately 500,000 hectares). As a response to these losses, the Kentucky Division of Water (KDOW) and Eastern Kentucky University (EKU) developed the Kentucky Wetland Rapid Assessment Method (KY-WRAM) to evaluate the condition of Kentucky's remaining wetlands. The goal of this study was to validate the KY-WRAM for forested riverine wetlands using a vegetation index of biotic integrity (VIBI), bird surveys, and landscape development index (LDI). Specific objectives of this study were to: 1) determine the correlation between bird species richness, VIBI, and LDI with the KY-WRAM in …
Effect Of Variation In Nestling Hunger Levels On The Begging Behavior Of Nestlings And The Provisioning Behavior Of Adult American Kestrels, Katheryn Ann Watson
Effect Of Variation In Nestling Hunger Levels On The Begging Behavior Of Nestlings And The Provisioning Behavior Of Adult American Kestrels, Katheryn Ann Watson
Online Theses and Dissertations
Little is known about how variation in nestling begging intensity influences the behavior of adult raptors and how responses of adult males and females to such variation might differ. My objective was to manipulate the begging intensity of nestling American Kestrels (Falco sparverius) and examine the responses of adult males and females. I studied 12 pairs of kestrels nesting in nest boxes from 1 March to 1 July 2014 at the Blue Grass Army Depot, Madison County, Kentucky. Nest boxes were modified with a separate compartment for a camcorder to record nestling behavior, and a second camcorder was placed outside …
Distribution, Movement, And Ecology Of Etheostoma Spilotum (Gilbert), The Kentucky Arrow Darter, In Gilberts Big Creek And Elisha Creek, Red Bird River Basin, Clay And Leslie Counties, Kentucky, Jonathan Bradley Baxter
Distribution, Movement, And Ecology Of Etheostoma Spilotum (Gilbert), The Kentucky Arrow Darter, In Gilberts Big Creek And Elisha Creek, Red Bird River Basin, Clay And Leslie Counties, Kentucky, Jonathan Bradley Baxter
Online Theses and Dissertations
The Kentucky Arrow Darter (KAD), Etheostoma spilotum, is an endemic species to the Upper Kentucky River Basin and is currently proposed for listing as threatened under the Endangered Species Act. The ecology and population status of this benthic species is poorly understood, so this study was designed to investigate the species’ movement capabilities, population dynamics, and overall ecology in two streams (Gilberts Big and Elisha Creek) in the Red Bird Ranger District, Daniel Boone National Forest, Kentucky. Project objectives included quantification of movement patterns, identification of microhabitat use, and estimation of population size in both streams. Sampling was conducted during …
Examining The Impacts Of Valley Fills In Stream Ecosystems On Amphibian And Aquatic Insect Communities In Southeastern Kentucky, John Clayton Bourne
Examining The Impacts Of Valley Fills In Stream Ecosystems On Amphibian And Aquatic Insect Communities In Southeastern Kentucky, John Clayton Bourne
Online Theses and Dissertations
Chapter 1. Abstract: Biodiversity is not evenly distributed, and understanding factors that determine spatial patterns of species diversity remains a key question in ecology. Because of their relatively high abundance and complex life cycles, stream salamanders and aquatic insects are important trophic links and serve a critical role in transferring energy. Despite this importance little research has examined their community structure simultaneously in aquatic ecosystems. The primary objective of this research was to determine the structure of these communities across natural areas of southeastern Kentucky and understand what factors impact their abundances and distributions. To address this, we sampled eight …
Wolf-Cougar Co-Occurrence In The Central Canadian Rocky Mountains, Ellen Brandell
Wolf-Cougar Co-Occurrence In The Central Canadian Rocky Mountains, Ellen Brandell
Undergraduate Theses, Professional Papers, and Capstone Artifacts
Cougars and wolves are top carnivores that influence the dynamics of an ecosystem, including prey behavior and dynamics, and interspecific competition. Studies about the interactions between wolves and cougars typically find wolves are dominant competitors to cougars. We examined single-species, single-season occupancy models and co-occurrence models of wolves and cougars in the Central Canadian Rocky Mountains to understand interactions between these two species on a grand landscape. Data was collected from 2012-2013 using remote wildlife cameras and separated into seasons. Naïve occupancy estimates were larger for wolves in both seasons, but both species had smaller ranges in winter. There were …
Space Use By Resident And Transient Coyotes In An Urban–Rural Landscape Mosaic, N. Mitchell, M. W. Strohbach, R. Pratt, W. C. Finn, Eric G. Strauss
Space Use By Resident And Transient Coyotes In An Urban–Rural Landscape Mosaic, N. Mitchell, M. W. Strohbach, R. Pratt, W. C. Finn, Eric G. Strauss
Center for Urban Resilience Scholarship
Context. Coyotes (Canis latrans) have adapted successfully to human landscape alteration in the past 150 years and in recent decades have successfully moved into urban areas. While this causes concern about human–wildlife conflicts, research also suggests that coyotes tend to avoid humans and human activity in urban areas. For improving management, a better understanding of space use by coyotes is needed.
Aims. To study how coyote social behaviour influences fine-scale space use in urban areas we present results from an extensive, multi-year GPS telemetry study (2005–13). The study area in coastal Rhode Island is a mosaic of rural, …
Context-Dependent Individual Variation In Foraging Behaviour And Parental Care In House Sparrows, David Moldoff
Context-Dependent Individual Variation In Foraging Behaviour And Parental Care In House Sparrows, David Moldoff
Theses and Dissertations--Biology
Behaviors can exhibit a wide degree of plasticity depending on the environmental context in which they are expressed. Despite this, repeatable differences have been found among and within individuals across a wide range of taxa. For my thesis, I investigated individual differences in foraging and parental care. In the first experiment, I assessed house sparrows (Passer domesticus) for domain-generality among neophobia, habituation and associative learning as they are all responses to novelty. While the results of the study find individual differences in each of these contexts the conclusion supported separate mechanisms for each response (domain-specificity). In the second experiment, I …
Evaluation Of A Formula That Categorizes Female Gray Wolf Breeding Status By Nipple Size, Shannon M. Barber-Meyer, L. David Mech
Evaluation Of A Formula That Categorizes Female Gray Wolf Breeding Status By Nipple Size, Shannon M. Barber-Meyer, L. David Mech
United States Geological Survey, Northern Prairie Wildlife Research Center: Publications
The proportion by age class of wild Canis lupus (Gray Wolf) females that reproduce in any given year remains unclear; thus, we evaluated the applicability to our long-term (1972–2013) data set of the Mech et al. (1993) formula that categorizes female Gray Wolf breeding status by nipple size and time of year. We used the formula to classify Gray Wolves from 68 capture events into 4 categories (yearling, adult non-breeder, former breeder, current breeder). To address issues with small sample size and variance, we created an ambiguity index to allow some Gray Wolves to be classed into 2 categories. We …