Ecology and Evolutionary Biology Commons^™

Beyond Genetics: Addressing Activity In Microbial Ecology, Scott Christopher Thomas

UNLV Theses, Dissertations, Professional Papers, and Capstones

The field of microbial ecology is on the cusp of another revolution, one that uses synergy to address the limitations of previous studies. My dissertation is representative of the new era of research to come, through combining the latest technological advances with established classical methods to provide a more complete understanding of microbial ecosystems. In my first study, I evaluate the conservation of central carbon metabolic pathways and activity along a temperature and diversity gradient (60-95 ℃) through the use of 16S rRNA gene amplicon data, metagenome sequencing, and position-specific 13C-labeled metabolite probing. In my second study, I provide the …

Larval-Ant Interactions In The Mojave Desert: Communication Brings Us Together, Alicia Mellor

UNLV Theses, Dissertations, Professional Papers, and Capstones

Butterflies are a diverse and essential group of pollinators whose abundance is predominantly determined by growth and survival of their larvae. In the family Lycaenidae, many species participate in a larval-ant mutualism where ants feed on nutrient-rich nectar produced by larvae and, in turn, protect those larvae from predators. Emerging evidence indicates larval scent in the form of cuticular hydrocarbons (CHCs), not nectar production, drives this interaction. This study takes two approaches to investigate CHCs as the driver behind the larval-ant mutualism in three butterfly species native to southern Nevada: Euphilotes bernardino martini (Martin’s blue), Brephidium exilis (western pygmy blue), …

Acute Salivary Steroid Hormone Responses During Coalitional And Dyadic Competitions In Hong Kong Juvenile Children, Timothy Mchale

UNLV Theses, Dissertations, Professional Papers, and Capstones

A large body of research links testosterone and cortisol responses to competition during adulthood. Little psychoneuroendocrine research has explored salivary steroid hormone responses to competition during middle childhood. This project investigated the relationship between acute salivary steroid hormone change, performance, competitor type, and outcome effects in three different field studies, while controlling for Body Mass Index (BMI) and pubertal development, in a population of ethnically Chinese, Hong Kong juvenile children, 8-11 years of age. The relative dynamics of salivary steroid change were assessed during a 1) coalitional physical competition (soccer) in boys, 2) a non-physical mixed-sex coalitional competition (math contest), …

Emerging Infectious Disease And The Imperiled Relict Leopard Frog, Anthony Wayne Waddle

UNLV Theses, Dissertations, Professional Papers, and Capstones

The disease chytridiomycosis, caused by the aquatic fungal pathogen Batrachochytrium dendrobatidis (Bd), has emerged as a major contributing factor for worldwide amphibian declines. Although relatively recently described, the impacts from the disease this pathogen causes have been definitively tied to amphibian declines, including some that occurred decades ago. In some cases, declines of individual species occurred with little documentation and are thus poorly understood. The relict leopard frog (Rana onca = Lithobates onca) has experienced such a decline and by the latter part of the 20th century only occurred in two general areas in southern Nevada. Recent research has found …

Thermal And Developmental Ecology Of Pupfish, Cyprinodon, Alexander Jones

UNLV Theses, Dissertations, Professional Papers, and Capstones

Pupfish from the genus Cyprinodon are among the most endangered groups of fishes on the planet, with nearly 40% of species being threatened with extinction. These pupfishes are often assumed to be the most temperature tolerant of all fish, coping with temperatures as low as 0 °C and as high as 44 °C. However, conflicting data exist and certain measures of thermal tolerance are not markedly higher in pupfish compared to common game fish. Pupfish egg production and growth has been known to be hampered at temperatures well below what they apparently experience in nature. I addressed why eggs fail …

Living On The Edge: Assessing The Effects Of Catastrophic Fire On Plants Utilized By Two Endemic Subspecies Of Spring Mountains Butterflies, Charles Ryan Herrmann

UNLV Theses, Dissertations, Professional Papers, and Capstones

In the past few decades there has been an increase in catastrophic, high-intensity, large-scale wildfires globally due to the combination of climate warming with more than a century of fire suppression policy. One region that has been drastically affected is the Western United States, as there has been an increase in ‘mega-fires’ in the past few decades. The 2013 Carpenter 1 Fire in the Spring Mountains, Nevada was the largest fire in recorded history in these mountains, spreading out over 11,137 hectares. Catastrophic fire like the Carpenter 1 Fire can have potentially devastating effects on endemic species inhabiting refugia on …

Behavioral Paleoecology Of Lower Cambrian Deposit Foragers: Reinterpreting Looping And Meandering Traces Using Optimal Foraging Theory And Quantitative Analysis, Zachary Andrew Jensen

UNLV Theses, Dissertations, Professional Papers, and Capstones

The early Cambrian was a period of transition during which the seafloor environment was changing from a microbial-mat-dominated environment to a bioturbation-dominated environment. The result was a patchy landscape of variable food resources for foraging burrowers to exploit. Looping trace fossils, such Psammichnites gigas and Taphrhelminthopsis nelsoni, appear in strata worldwide during this transitional period, and the ecological niche they filled is a subject of debate among ichnologists. The objectives of this study are (1) to determine the foraging strategies preserved by looping traces through the application of optimal foraging theory and quantitative analysis, and (2) use those results to …

Sexual Determination From Frontal Sinus Analysis In A Subadult Population Using Archival Radiographic Records, Erica Crosta

UNLV Theses, Dissertations, Professional Papers, and Capstones

The goal of this study is to use the improved imaging capability of cone-beam computerized tomography (CBCT) to investigate the development and sexual dimorphism of the frontal sinus and surrounding supraorbital region in the subadult population of urban Southern Nevada. CBCT radiographs were obtained from the UNLV School of Dental Medicine archival dental records. Five hundred and fifty six of these radiographs were reviewed for the study. Two hundred and sixteen patients (92 males, 124 females) between the ages of 7 and 20 years were included based on inclusion/exclusion criteria. Samples were categorized into 3 subadult age groups for analysis: …

Behavioral Reactions To Emotional And Physical Infidelity: An Evolutionary Perspective, Mandy Walsh Walsh

UNLV Theses, Dissertations, Professional Papers, and Capstones

This thesis examined the effects of sex and type of partner infidelity, including both physical (i.e., sexually involved with another person) and emotional (i.e., emotionally involved with another person) infidelity, on mate abandonment behaviors. Previous research has demonstrated sex differences in emotional responses to infidelity, where men react more negatively to physical infidelity and women react more negatively to emotional infidelity. While various studies have investigated perceived behavioral reactions using imagined scenarios, this study expands current research by utilizing actual retrospective reports. It was hypothesized that males would engage in significantly more mate abandonment behaviors after experiencing a physical infidelity, …

Incomplete Denitrification In Thermus Species, Chrisabelle Mefferd

UNLV Theses, Dissertations, Professional Papers, and Capstones

Members the bacterial genus Thermus have been shown to be incomplete denitrifiers, terminating with nitrite or nitrous oxide (N2O). However, the ability to carry out denitrification and the evolution of nitrogen oxide reductase genes in Thermus remains poorly understood. This study tests the hypothesis that incomplete denitrification is common in Thermus and seeks to uncover patterns in the evolution of denitrification pathways in Thermus. Denitrification capacity was determined in a collection of 25 strains representing ten species of Thermus and phylogenetic analysis was performed to determine whether denitrification genes evolved horizontally in Thermus. No strains in this study reduced nitrate …

Water Source Partitioning For Shrubland Transpiration In A Mixed Phreatophytic Plant Community, Amanda Jean Wagner

UNLV Theses, Dissertations, Professional Papers, and Capstones

Groundwater in the basins of east central Nevada (Great Basin Desert, NV) has been permitted for export to southern Nevada. These basins are also vulnerable to changes in climate that could lead to declining recharge. Any decline in groundwater levels no matter the driving force could significantly impact phreatophytes (deep rooted shrubs), which utilize water from both the vadose zone and the capillary fringe to meet plant water needs, to support growth, and for overall health. A reduction in groundwater due to these forces could have a significant impact on the decoupling of phreatophytes from what is normally a reliable …

Physiology And Genetics Of Starvation-Selected Drosophila Melanogaster, Christopher Michael Hardy

UNLV Theses, Dissertations, Professional Papers, and Capstones

In nature, organisms have evolved to survive in stressful environments. This has driven organisms to adopt a wide range of unique adaptations. Investigating the mechanistic basis of these adaptations is an important tool for discovery that has led to major advances in science and medicine.

We study how organisms survive life without food, or starvational stress. Environmental stressors have shaped the quantity and quality of food sources across the globe. This has led to vast differences in the ability of some organisms to tolerate starvation over others. Many researchers have used Drosophila melanogaster as a model to study global patterns …

Assessing The Importance Of Nurse Plant Associations To The Growth Of Pre-~Reproductive Yucca Brevifolia, Eric James Chameroy

UNLV Theses, Dissertations, Professional Papers, and Capstones

Facilitation and competition among plant species, in addition to abiotic factors, play an important role in determining plant community structure in arid and semi-arid environments. I conducted a study in Dry Lake Valley, Lincoln County, Nevada, USA to investigate the importance of nurse plant associations to pre-reproductive Yucca brevifolia (Joshua tree). Dry Lake Valley lies within a transition desert between the Mojave and Great Basin Deserts with ecotonal plant communities consisting of species representative of both deserts. A vegetation survey described the communities in which this study was conducted as a Y. brevifolia woodland dominated by an understory of Ephedra …

Stable Isotope Analysis Of Bison Latifrons And Paleoecological Inferences, Fabian Ceron Hardy

UNLV Theses, Dissertations, Professional Papers, and Capstones

Bison latifrons was a large Pleistocene herbivore that is traditionally hypothesized to have been adapted to living in forest openings and woodlands. According to this view, the species was primarily a browser of high-growing, woody plants. Very little isotopic work has been conducted on this species, and there have been no prior studies of high altitude localities containing this species. This study aims to address both of these issues.

B. latifrons is known from sites in several states, including California, Idaho, Colorado, Nevada, and Florida, among others. These sites provide diverse examples of this species’ habitat and the opportunity to …

Decontamination Protocols For Watercraft And Wildland Firefighting Equipment In Preventing The Spread Of Invasive Quagga (Dreissena Rostriformis Bugensis) And Zebra (Dreissena Polymorpha) Mussels, Ashlie Watters

UNLV Theses, Dissertations, Professional Papers, and Capstones

Quagga and zebra mussels (Dreissena rostriformis bugensis and Dreissena polymorpha) are two invasive species introduced via ballast water discharged by large oceanic cargo ships to the North American Great Lakes in the late 1980s. Once established, the mussels spread quickly. In January 2007, D. rostriformis bugensis was discovered in Lake Mead, NV-AZ, and in that same year, mussels were confirmed further south on the Colorado River in Lakes Mojave and Havasu. Dreissenids clog water intake pipes, water filtration systems, and electric generating plants. The mussels also ruin boat motors, damage recreational equipment, and once established in the reservoir, routine maintenance …

Prymnesium Parvum And Fish Kills In A Southern Nevada Man-~Made Reservoir, Tara Gregg

UNLV Theses, Dissertations, Professional Papers, and Capstones

The water chemistry of Lake Las Vegas (LLV), a Southern Nevada man-made reservoir, is influenced by both anthropogenic and natural sources. These sources affect the reservoir's water chemistry, which may promote harmful algal blooms (HABs) leading to massive fish kill events. Fish kills, caused by the golden algaePrymnesium parvum (P. parvum), continue to pose a threat in the reservoir. However, no effective treatments to controlP. parvumin large reservoirs such as LLV have been determined. This cross-sectional study evaluated important variables that may affectP. parvumHABs in LLV, including non-P. parvumbiomass (mg/m3), total zooplankton abundance (#/m3), temperature, TDS (salinity), mineral concentration (Ca, …

Pronghorn (Antilocapra Americana) Offspring Recruitment On The Carrizo Plain National Monument: Evaluating The Effects Of Low Population Density And Marginal Habitat Quality., Diego Ramirez Johnson

UNLV Theses, Dissertations, Professional Papers, and Capstones

Demographic fluctuation among ungulate populations is strongly linked to variability in recruitment. Rates of recruitment are subject to various forms of density-dependent and density-independent regulation. For species which benefit from the presence of conspecifics, reduced population density can decrease rates of recruitment and trigger a decline in per capita growth. Termed the Allee effect, this scenario can cause demographic collapse and population extinction. For many ungulate species, predation on juveniles is reduced when the timing and distribution of births is synchronized within a local population. Because birth synchrony is density-dependent, it may act as a mechanism for the Allee effect …

Assessing Growth Response To Climate Controls In A Great Basin Artemisia Tridentata Plant Community, Lorenzo F. Apodaca

UNLV Theses, Dissertations, Professional Papers, and Capstones

An assessment of the growth response of key vegetative species to climatic variability is vital to identifying possible local impacts on ecosystems faced with imminent climate change. With current climate projections in Nevada predicting a shift to an even more arid climate with greater year-to-year variability, the imperative exists to identify the effects of specific climatic controls on plant growth and to research methods to assess large-scale vegetative changes, especially in more remote areas where readily available data sets may be lacking. This study utilized annual growth ring indices constructed from big sagebrush (Artemisia tridentatassp.tridentata) stems collected in Spring Valley, …

Time-Dependent Random Effect Poisson Random Field Model For Polymorphism Within And Between Two Related Species, Shilei Zhou

UNLV Theses, Dissertations, Professional Papers, and Capstones

Molecular evolution is partially driven by mutation, selection, random genetic drift, or combination of the three factors. To quantify the magnitude of these genetic forces, a previously developed time-dependent fixed effect Poisson random field model provides powerful likelihood and Bayesian estimates of mutation rate, selection coefficient, and species divergence time. The assumption of the fixed effect model that selection intensity is constant within a genetic locus but varies across genes is obviously biologically unrealistic, but it serves the original purpose of making statistical inference about selection and divergence between two related species they are individually at mutation-selection-drift inequilibrium. By relaxing …

Ichnology And Paleoecology Of The Jurassic Aztec Sandstone, Heather Marie Stoller

UNLV Theses, Dissertations, Professional Papers, and Capstones

In this study I describe and interpret tracks and trackways of the Jurassic Aztec Sandstone of southern Nevada and southern California. This study involved mapping of all known tracks and trackways, including foot length, stride length, and trackway width. Photogrammetric data, collected by Bureau of Land Management scientists, were utilized for several trackways in Red Rock Canyon National Conservation Area.

More than three hundred tracks belonging to five ichnotaxa were documented within the Aztec Sandstone, including about 165 tridactylGrallator, 250 tetradactyl (four-toed)Brasilichniumtracks, and 7 arthropod trackways ofOctopodichnusandPaleohelcura. Four of the five ichnotaxa were not previously reported from the Aztec Sandstone. …

Paleoecology Of Late Pleistocene Megaherbivores: Stable Isotope Reconstruction Of Environment, Climate, And Response, Aubrey Mae Bonde

UNLV Theses, Dissertations, Professional Papers, and Capstones

Late Pleistocene megaherbivore communities of the Pacific and Mountain West states of California and Nevada are under-analyzed in regard to ecological function (diet, mobility, niche partitioning, and range of ecological tolerance). Stable isotope analysis is a powerful tool that is known to recover primary paleodiet and paleoenvironmental information from biogenic materials, such as enamel and dentin. This dissertation explores the use of carbon and oxygen stable isotopes in Late Pleistocene (40-10 Ka) megaherbivore teeth to gain a better understanding of inter- and intra-specific behavior and reconstruct Late Pleistocene ecosystems of California and Nevada. Radiocarbon dates exist for most of the …

Understanding Basin Specific Life History Characteristics Of Lake Mead Quagga Mussels (Dreissena Bugensis) And A Potential Treatment Using Uv Radiation In Laboratory Studies, Melissa Thaw

UNLV Theses, Dissertations, Professional Papers, and Capstones

The quagga mussel (Dreissena bugensis) is an aquatic invasive species that is spreading throughout Lake Mead and other western waterways. Unlike their native waters in Eurasia, Lake Mead exhibits year round warm temperatures, high calcium levels and a lack of natural predators, all of which are very favorable conditions for their growth and spread. Dreissena bugensis reproduce and colonize hard surfaces rapidly, where they filter large amounts of water. They disrupt the aquatic food chain and interfere with infrastructure that is exposed to lake water. There is an urgent need to understand Dreissena bugensis life history characteristics within this new …

Integrating, Developing, And Testing Methods To Generate More Cohesive Approaches To Biogeographic Inference, Mallory Elizabeth Eckstut

UNLV Theses, Dissertations, Professional Papers, and Capstones

As a fundamental component of the developing discipline of conservation biogeography, broadscale analyses of biotic assembly and disassembly across multiple temporal and spatial scales provide an enhanced understanding of how geologic transformations and climate oscillations have shaped extant patterns of biodiversity. As with any scientific field, there are limitations in the case of biogeographic historical reconstructions. Historical reconstructions are only as robust as the theoretical underpinnings of the methods of reconstruction (including data collection, quality, analysis, and interpretation). Nevertheless, historical reconstructions of species distributions can help inform our understanding of how species respond to environmental change.

My dissertation takes a …

Dispersal Ecology Of Desert Mosses Along Gradients Of Elevation, Wildfire Disturbance And Local Niche, Robert Joseph Smith

UNLV Theses, Dissertations, Professional Papers, and Capstones

Much of the variation in plant communities can be explained by the dispersal of individuals across landscapes, an ecological process that contributes to clinal variation, post-disturbance recovery and habitat occupancy. The role of dispersal is of particular concern for Mojave Desert plant communities that may not be able to tolerate recent departures from historical fire regimes. The aim of this thesis was to infer how dispersal is reflected by patterns of diversity in disturbed and undisturbed bryophyte communities in the Spring Mountains of southern Nevada. Chapter 2 presents an examination of sub-surface bryophyte communities (propagule "soil-banks") along a 1400 m …

The Evaluation Of Water Storage In Death Valley Using Grace Satellite Data, Maile Sweigart

UNLV Theses, Dissertations, Professional Papers, and Capstones

As drought conditions spread across the United States, concerns over water supplies, water use, and water management policies are growing and possible contributing environmental factors are continually being scrutinized. This thesis examines Death Valley as an analog for Southern Nevada and utilizes NASA EOS data, combined with ancillary climate data, to assess the effect of decadal climate variability on groundwater storage in the Death Valley area. Historical climate data, combined with satellite imagery observations, were compiled and calculated for analyses. Conclusions derived from statistical analyses infer trends between GRACE (Gravity Recovery and Climate Experiment) satellite data and fluctuating levels of …

Understanding Effects Of Changes In Land Use, Environmental Parameters, Habitat Characteristics On The Great Basin Aquatic Invertebrates Using Ecological Stoichiometric Theory, Knut Mehler

UNLV Theses, Dissertations, Professional Papers, and Capstones

Many freshwater ecosystems in the Great Basin have undergone dramatic alteration over the past decades due to groundwater withdrawal, water diversion and changes in land use. These changes have affected benthic food web dynamics by altering the availability of chemical key elements for primary and secondary producers, by loss of biodiversity and extinction of endemic species. However, only few studies are available that addresses the link between changes in land use, habitat characteristics and its effects on benthic macroinvertebrates (BMI) communities comprehensively and little attention has been given to integrating physiochemical aspects to link ecosystem functions.

This dissertation analyzes the …

The Effects Of Starvation Selection On Drosophila Melanogaster Life History And Development, Lauren A. Reynolds

UNLV Theses, Dissertations, Professional Papers, and Capstones

In nature, animals may endure periods of famine to complete their life cycles. Starvation stress will increase in populations as climates around the world change. To predict how populations may respond to such a stress, laboratory experimentation becomes essential. The evolutionary process of adaptation, its innovations and their trade-offs, can be studied in populations experiencing starvation stress. For this purpose outbred populations ofDrosophila melanogasterwere selected for starvation resistance in the laboratory.

After 60+ generations of starvation selection the starvation-selected flies have gone from surviving 2-3 days without food to 12-14 days without food. How this amazing feat of resistance is …

Soil Moisture Recognition And The Spatial Distribution Of Storm Activity In The Mojave Desert Using High-Resolution Aster And Modis Imagery For Thermophysical Mapping, Russell J. Skuse

UNLV Theses, Dissertations, Professional Papers, and Capstones

Climate models suggest that the Mojave Desert ecoregion is vulnerable to becoming drier in the future, and as the human population grows and development increases, environmental stresses will likely increase. Determining the spatial distribution and variation of soil moisture on a regional scale is an essential component to climate change, hydrologic, and habitat analyses. Soil permeability and sediment stability are characteristics that have been shown to be measurable from remote sensing observations. The primary objective of this project is to map the mechanical composition of the surface materials in the Mojave Desert ecoregion with implications for soil permeability, sediment stability, …

Restoration Of Biological Soil Crust On Disturbed Gypsiferous Soils In Lake Mead National Recreation Area, Eastern Mojave Desert, Lindsay P. Chiquoine

UNLV Theses, Dissertations, Professional Papers, and Capstones

Biological soil crusts (BSCs) are sensitive to anthropogenic disturbances. Natural recovery takes many years. Active restoration decreases recovery time. Native BSC inocula, which included lichens and mosses, salvaged from gypsiferous soil habitats in Lake Mead National Recreation Area (LMNRA) in the eastern Mojave Desert were stored dry for two years and applied to disturbed soil after a road reconstruction in LMNRA and also used in laboratory experiments to test inoculation technique effectiveness. After 18 months, field results revealed positive relationships between inoculation and the presence of macroscopic BSC cover, cyanobacteria abundance, soil stability, and ammonium concentrations. Chlorophyll fluorescence monitoring of …

The Abundance And Distribution Pattern Of Quagga Mussels In The Las Vegas Bay Of Lake Mead, Nevada And Potential Correlation With Environmental Parameters, Scott Rainville

UNLV Theses, Dissertations, Professional Papers, and Capstones

The non-native quagga mussel [Dreissena bugensis (Andrusov, 1897)] was initially discovered in Boulder Basin of Lake Mead, Nevada-Arizona on January 6, 2007. This occurrence marked the first biological invasion of dreissenid mussels in the Western U.S., following a significant North American range expansion of invasive quagga mussels from populations that invaded and colonized the Great Lakes Region in the Northeastern U.S. during 1991. This nonindigenous mollusk species quickly spread from Boulder Basin and became established throughout the entire reservoir by the end of 2007, with the exception of the inner Las Vegas Bay. There was an apparent absence of settled …