Ecology and Evolutionary Biology Commons^™

The Natural Life History Of Cambarus (Puncticambarus) Smilax, The Greenbrier Crayfish, Paul William Hughes

Theses, Dissertations and Capstones

The natural life history of Cambarus (Puncticambarus) smilax, the Greenbrier Crayfish, was studied in West Fork of the Greenbrier River and in Thorny Creek, a tributary of the main stem of the Greenbrier River. The Greenbrier Crayfish gets its name from the Greenbrier River watershed where it is thought to occur exclusively. Among described members of the subgenus Puncticambarus, C. smilax is a sister taxon most similar to Cambarus (Puncticambarus) robustus. Monthly collections were made within the two study sites, from August 2010 to July 2011. Collecting techniques included dip-netting, seine-netting, and hand collecting. Cambarus smilax and all other species …

Natural History Of A Population Of Wood Turtles (Glyptemys Insculpta) In West Virginia, Jessica Curtis

Theses, Dissertations and Capstones

The Wood Turtle (Glyptemys insculpta) is listed as vulnerable in West Virginia and only occurs in the Eastern Panhandle. The study population is unique in West Virginia because it occurs at a much higher mean elevation than other Wood Turtle populations in the state. The primary objective of this study was to determine if, and to what extent, altitude affected this population’s ecology. Turtles were located with opportunistic surveys and captured by hand. I located 31 turtles: 18 males, and 13 females. Turtles were captured a total of 68 times. Ratio of males to females did not differ significantly from …

Assessing Grazing Impacts On A Tropical Dry Forest System In Madagascar Through Vegetation, Satellite Image, Lemur Occupancy, And Acoustic Analysis, Lyndsay Lee Rankin

Theses, Dissertations and Capstones

The ability to assess the quality or ecological value of a landscape is a useful tool in effective conservation and natural resource management. Ecosystem biodiversity and functionality are reduced when human activities cause habitat alteration and/or fragmentation. Compromised habitats are unlikely to support naturally occurring communities or healthy ecosystem functions. Conservation scientists have historically concentrated their efforts on large, intact habitats with little human disturbance; the conservation value of small and/or disturbed areas has been comparatively overlooked. One limitation may be a lack of tools to identify disturbed areas with conservation value. Techniques that allow researchers to assess the habitat …

Effectiveness Of Manual Palpation In The Northern Water Snake, Nerodia Sipedon Sipedon, As A Method To Extract Gut Contents For Dietary Studies, Marcella Ann Cruz

Theses, Dissertations and Capstones

The Common Water Snake, Nerodia sipedon sipedon, is one of six subspecies, and the only species of Nerodia to inhabit West Virginia. Because it is abundant and information on the species is lacking within the state, it was used as my study organism. The effectiveness of manual palpation, the most commonly used method in snake diet related studies, was tested against museum dissection by comparing presence and absence of gut contents. Nineteen individuals of 76 manually palpated collected snakes were compared to 6 individuals of 37 dissected snakes using a Pearson chi-square analysis. Results suggested manual palpation is an effective …

Examining Habitat Selection And Home Range Behavior At Multiple Scales In A Population Of Eastern Box Turtles, (Terrapene C. Carolina), With Notes On Demographic Changes After 17 Years, Brian Anthony Williamson

Theses, Dissertations and Capstones

The Eastern Box Turtle, Terrapene c. carolina, is a terrestrial species native to the eastern United States. Once considered common, it is currently declining in much of its range due to habitat destruction and disease. To conserve the species, knowledge of Eastern Box Turtle habitat selection and the factors influencing their movement is necessary. Although the home range behavior of the Eastern Box Turtle has been well studied, few studies have quantified habitat selection at the home-range scale. Therefore, I examined home-range selection and within home range habitat selection in a population of Eastern Box Turtles at Hungry Beech Nature …

Filling In The Gaps In Phenology And Life History Of The Cumberland Plateau Salamander (Plethodon Kentucki), Robert C. Bowers

Theses, Dissertations and Capstones

The Cumberland Plateau Salamander, Plethodon kentucki, is a member of the Plethodon glutinosus complex comprising 16 sibling species, which are best differentiated by range. Few studies have been conducted to gain information on the natural history of P. kentucki. To alleviate this, two sites at Beech Fork State Park in Wayne County, West Virginia were used to study the salamander’s general life history with emphasis on reproduction, phenology, and population size. At each site, three 20m x 20m sample plots were arranged based on viability of the habitat for P. kentucki. Ground searches of all cover objects …

Examining The Influence Of Mating Systems On Testes Size In Salamanders, Howard James Stanton Ii

Theses, Dissertations and Capstones

Sperm competition theory predicts that relatively larger testes sizes evolve in animals with polygamous mating systems compared to those in monogamous mating systems due to sperm competition. Whereas intensity of sperm competition is the significant predictor of testes sizes in other taxa such as mammals, frogs, birds, insects, and fish, in salamanders the intensity of male-male competition in the transfer of spermatophores to females is predicted to be a critical factor. This is because males have to deposit more spermatophores to secure reproductive pay-off under higher intensity of male-male competition. I hypothesized that salamander species that breed explosively as groups …

Quantitative Reconstruction And Two-Dimensional, Steady Flow Hydrodynamics Of The Plesiosaur Flipper, Mark Cruz Deblois

Theses, Dissertations and Capstones

Plesiosaurs are a group of extinct marine reptiles that thrived during the Mesozoic Era. They are unique for swimming with two hydrofoil-shaped flippers. Penguins, sea turtles, and cetaceans all have hydrofoil shaped flippers but penguins and sea turtles only use the front pair to produce thrust and cetaceans use their tail flukes. Consequently, the mode of swimming for plesiosaurs has long been debated. However, a quantitative study of the hydrodynamic properties of the flippers, which would constrain inference about their mode of swimming, has not yet been done. The main reason is that the trailing edge of the plesiosaur flipper …

Ontogenetic State Of A Juvenile Polycotylid Plesiosaur (Sauropterygia: Plesiosauria) And Its Implications For Plesiosaur Growth, Christina Joanne Byrd

Theses, Dissertations and Capstones

Understanding ontogenetic variation in plesiosaurs from the family Polycotylidae can provide insight into the evolutionary history of this group of extinct short-necked marine reptiles from the Cretaceous period. In this study, I analyzed a juvenile polycotylid plesiosaur from the University of Nebraska State Museum (UNSM 55810). UNSM 55810 came from the Pierre Shale (99-65 Ma) of Nebraska and possesses skeletal elements that include a partial skull, nearly complete pectoral and pelvic girdles, and an assortment of paddle elements. Based on several cranial characteristics, this specimen is probably referable to the genus Dolichorhynchops. Previous qualitative studies have included UNSM 55810 but …

Size And Age Variation Of Larval Gyrinophilus Porphyriticus Porphyriticus In Sympatry With Salvelinus Fontinalis, Aaron Jacob Semasko

Theses, Dissertations and Capstones

The Northern Spring Salamander (Gyrinophilus porphyriticus) has a larval stage that could extend up to six years, an anomaly unheard of in other plethodontid salamanders. The size of a larva could be larger than an adult of a younger age. A sympatric species, Salvelinus fontinalis, the native brook trout, negatively alters the individual mass and survival of G. p. porphyriticus larvae. In the absence of trout, the role of G. porphyriticus ascends to top predator in a small stream community. Amphibians specifically have proven their role as stream indicators, a position attributed to their porous skin. I attempted to determine …

Dietary Preference Of The Queensnake (Regina Septemvittata), Timothy J. Brust

Theses, Dissertations and Capstones

The Queensnake (Regina septemvittata) is a small secretive water snake found throughout the eastern United States. Once common, their numbers have declined to the extent that they are now threatened throughout most of their range, largely the result of pollutant-based reduction in prey species. These snakes are assumed to eat molted crayfish exclusively. For some common crayfish species, molting happens only twice a summer during a two- week period. It has not been documented if Queensnakes eat anything besides crayfish on a regular basis. The purpose of this study was to determine the prey preference of Queensnakes with particular focus …

The Crayfishes Of West Virginia’S Southwestern Coalfields Region With An Emphasis On The Life History Of Cambarus Theepiensis, David Allen Foltz Ii

Theses, Dissertations and Capstones

Crayfish are the third most endangered faunal group in the world behind freshwater snails and unionid mussels. A better understanding of each species’ life history is vital in order to aid in crayfish conservation; however, little to no life history information is available for most crayfish. Recently, an undescribed species of crayfish, Cambarus theepiensis, was discovered in the Cumberland Mountains of West Virginia and Eastern Kentucky. In conjunction with the species’ scientific description, life history data was collected from May 2012 through April 2013 from two sites located within the Twelvepole watershed of southwestern West Virginia. Animals were collected monthly …

Diversity Of The Ohio River Bacterial Communities Using Next-Generation Sequencing Techniques, Emily Michele Anneken

Theses, Dissertations and Capstones

The ability to sequence bacterial genetic material directly from environmental samples has unlocked the field of metagenomics. Next-generation sequencing techniques, such as pyrosequencing, have been instrumental in providing knowledge about bacterial communities. This study focused on identifying the bacterial diversity of the Ohio River via pyrosequencing and compared the diversity of cultivable bacteria versus the entire river community. To ensure the maximum number of cultivable bacteria was obtained, cultures were grown on nonselective media and incubated at river temperature. Bacterial DNA was extracted, sequenced, and classified. The dominant phyla for the Ohio River included Cyanobacteria (38-66% of the total), Actinobacteria …

The Effect Of Auditory Call Playback On Anuran Detection And Capture Rates, Derek Adam Bozzell

Theses, Dissertations and Capstones

Calls of male anurans during breeding seasons are species-specific identification tools. However, males cease calling after any nearby disturbance, including those of researchers. I proposed a variation on current methods that attempts to reduce this lag in calling after researcher-created disturbance by utilizing the propensity for competition in male frogs. I surveyed 14 breeding sites in Cabell and Wayne counties during the 2010 and 2011 breeding seasons. First, I used traditional visual encounter surveys (VESs). After using automated recording devices to gather site-specific recordings of calls of all species present, I conducted secondary VESs while playing these playlists over a …

Freshwater Mussel Populations Of The Monongahela River, Pa And Evaluation Of The Orsanco Copper Pole Substrate Sampling Technique Using G.I.S. Interpolation With Geometric Means, Jonathan Hart

Theses, Dissertations and Capstones

Large river studies for freshwater mussel populations and habitat in the Monongahela River within Pennsylvania have been almost non-existent over the past century. Aquatic diversity and water quality have been impaired in the Monongahela River since the Industrial Revolution and early impoundments were constructed to control the river. To date, there have been no thorough mussel population studies conducted on the Pennsylvanian Monongahela River proper since A.E. Ortmann in 1919. The mussel population accounts for this large river system are invaluable accounts of the aquatic condition of the Monongahela River. Mussel populations and habitat within the river have diminished drastically …

The Status And Distribution Of Invasive Crayfishes And Their Effects On Native Crayfish Communities In West Virginia, Casey David Swecker

Theses, Dissertations and Capstones

Introductions of non-native crayfish species have resulted in the global decline of native crayfish populations, including those in North America. The last large survey of crayfishes from West Virginia was in 1988 and 1989. In this thorough document Raymond Jezerinac, Whitney Stocker, and Donald Tarter identified three separate areas in West Virginia where non-native crayfish species have been introduced. One area is located in the Potomac River drainage in northeastern West Virginia commonly referred to as the eastern panhandle of the state. This is also the only drainage in West Virginia where the native spiny cheek crayfish (Orconectes limosus) occurs. …

Chronic Toxicity Testing In Mining Influenced Streams Of West Virginia, Leah J. Bitzer

Theses, Dissertations and Capstones

Whole effluent toxicity (WET) tests have become a common tool in the evaluation of effluent for discharge acceptability. In this study, four years of toxicity data from 119 sampling locations were analyzed to determine relationships with ions and conductivity as indicators of toxicity. West Virginia Stream Condition Index (WVSCI) scores were also examined to evaluate correlations between stream scores, conductivity, and IC25 endpoints from toxicity results. Conductivity was not an indicator of toxicity in the range of conductivities tested. Streams dominated by mining effluent sometimes exhibited toxicity to Ceriodaphnia dubia; however, toxicity was not found to be related to ionic …

Plesiosaur Body Shape And Its Impact On Hydrodynamic Properties, Courtney D. Richards

Theses, Dissertations and Capstones

Despite the variability of cross-sectional body shape within Plesiosauria, its impact on plesiosaur buoyancy and stability has never been investigated. This study focused on Tatenectes, Cryptoclidus, and Muraenosaurus due to their variable body morphologies. Reconstructions were created based on measurements and photographs from fossil remains. The ability of computer models, based upon the reconstructions, to reach equilibrium after submersion, sink via lung deflation, and recover from a lateral roll was tested. For the computer models, Muraenosaurus was replaced with Thalassomedon, which had a similar morphology. Cryptoclidus and Thalassomedon recovered from submersion faster than Tatenectes. All models achieved negative buoyancy with …

The Little Coal River Improvement Project, West Virginia: An Initial Study Of Sediment, Bacteria And Benthic Macroinvertebrates, Emily Vargo

Theses, Dissertations and Capstones

The Little Coal River, located in southern West Virginia, has undergone many changes over the last few years in an effort to reverse the negative effects of various point source and non-point source pollutions. Marshall University, in cooperation with the West Virginia Department of Environmental Protection, Army Corp of Engineers, and the Coal River Group, has designed and implemented a plan for rehabilitation known as the Little Coal Improvement Project. Several improvement structures were strategically installed within the Little Coal River during the summer of 2007. The functions of these structures include sediment flushing and overall habitat improvement. Monitoring these …

Phylogenetic Analysis Of Blacknose Dace (Rhinichthys) In West Virginia Streams, Samantha Taylor

Theses, Dissertations and Capstones

Blacknose dace (Rhinichthys) are one of the most common cyprinid fishes in eastern North America. They also have been a topic of debate for over 30 years because morphology-based systematics has failed to clearly define their taxa. Taxonomists classify the complex into two species and one subspecies: the eastern form, R. atratulus atratulus; and the western form R. obtusus obtusus, and southern form R. obtusus meleagris. This research uses the mitochondrial cytochrome b gene and genomic RAG 2 gene in a phylogenetic analysis to help clarify species relations according to differences between each current species. Maps have been created to …

A Virtual Phytosaur Endocast And Its Implications For Sensory System Evolution In Archosaurs, Waymon Holloway

Theses, Dissertations and Capstones

Due to the overall morphological similarities between the Triassic archosaurs of the order Phytosauria and extant crocodilians, most studies have assumed that the two shared similar lifestyles. Many studies involving phytosaurs have focused on the external cranial morphology of various taxa. Internal cranial anatomy has received relatively little attention. As a result, comparative morphology studies of the braincases interior, or endocast, of phytosaurs are an area of potential exploration. Just as modern medical Xray computed tomography (CT) can be used to create three-dimensional images of internal structures of living subjects, such technology offers a non-invasive means of studying the internal …

The Natural History & Distribution Of Riverine Turtles In West Virginia, Linh Diem Phu

Theses, Dissertations and Capstones

Turtles are unique evolutionary marvels that evolved from amphibians and developed their protective shelled form more than 200 million years ago. In West Virginia, there are 10 native species of turtles, 9 of which are aquatic. Most of these aquatic turtles feed on carrion and dead plant matter, in the water and essentially "clean" our water systems. Turtles are long-lived animals with sensitive life stages that can serve as both long-term and short-term bioindicators of environmental health. With the increase in commercial trade, habitat fragmentation, degradation, destruction, there has been a marked decline in turtle species. The need for well …

The Coal River Basin: A 2009 Water Budget Study, R. Dale Biller

Theses, Dissertations and Capstones

The Coal River Watershed covers an area of some 890 square miles in southern West Virginia of which 863 square miles was investigated in this study. Both online and field data were collected over period from January 13, 2009 to January 13, 2010. The basin was studied as a closed system having an input, which was precipitation, and outputs, rainfall interception, evapotranspiration, and stream discharge that was separated into base flow and overland flow.

The effective rainfall of the watershed was calculated using the Thiessen polygon method to be 42 inches for the year, 48% of that water discharged by …

Effect Of Snake Populations On Salamanders As A Result Of Forest Fragmentation, Casey Renee Bradshaw

Theses, Dissertations and Capstones

Forest fragmentation is one of the main causes for the loss of native biodiversity. One consequence is increased proportion of edge habitat that introduces new “edge” species, and makes habitat for interior forest-living species less-suitable. This study was conducted at three sites in Tucker County, West Virginia and included one downhill ski slope, one cross country ski slope, and one gravel road. The main objectives of this study were to determine relative abundance of snake communities, how far species move from edge habitat into the forest and to determine whether snakes are a predatory threat to salamanders, specifically the federally …

Testing The Behavioral Responses Of West Virginia Turtles To Roads And Vehicles, Aaron Clinton Gooley

Theses, Dissertations and Capstones

Road mortality has been identified as a major threat to many turtle species; however, response to passing vehicles, crossing speed, and general behavior while crossing roads has never been investigated in turtles. To investigate these factors, Midland Painted Turtles (Chrysemys picta marginata), Eastern Box Turtles (Terrapene c. carolina), and Stinkpots (Sternotherus odoratus) were collected and placed in a release box on the side of a closed road with an active road running parallel to it 26 m away. Turtles were released via a pulley-operated door facing the road, and their actions videotaped by an …

Long-Term Growth And Monitoring Of The Eastern Hellbender (Cryptobranchus A. Alleganiensis) In Eastern West Virginia, Douglas Charles Horchler

Theses, Dissertations and Capstones

Amphibian declines have been well documented, specifically in the last few decades. The Hellbender, Cryptobranchus alleganiensis, one of North America’s largest salamander species, has suffered dramatic declines throughout much of its range, with estimated declines of up to 77 percent recently documented in some populations. With diurnal and nocturnal searches and mark-recapture techniques, I collected data on the status of Eastern Hellbender populations in Eastern West Virginia. We re-sampled a study site on the West Fork of the Greenbrier River that was first examined in 1998 by Jeff Humphries. Long-term growth and survivorship data were collected and compared to 1998 …

Potential Of Urban Habitats As Reptile And Amphibian Refuges In West Virginia, Scott Jones

Theses, Dissertations and Capstones

Urban herpetology is a relatively new field, examining how reptiles and amphibians survive in areas that have been altered by humans. This study sought to add data on urban habitats to the knowledge within West Virginia. I studied six sites: two urban parks (Ritter Park and Barboursville Park), a nature area near an art museum (Huntington Museum of Art), two wildlife management areas (Green Bottom WMA and Chief Cornstalk WMA), and a state park (Beech Fork State Park). The state park and two wildlife management areas were considered non-urban habitats because they are more removed from developed areas than the …

A Natural History Study Of Bufo A. Americanus, The Eastern American Toad, And The Phenology Of Spring Breeders In Southwest West Virginia, Tomi Maria Bergstrom

Theses, Dissertations and Capstones

Natural history traits have not been thoroughly collected and analyzed on the "common hoptoad," Bufo a. americanus (eastern American toad) in West Virginia. Updating natural history information is important to understand the present population status (Jackson, 2001). I chose to have two study sites at opposing physiographic regions to constitute a better understanding of B. a. americanus natural history in WV. The study was initiated in spring 2008 at Beech Fork State Park and Green Bottom Swamp Wildlife Refuge (in Mason and Cabell counties) and in 2009 incorporated Canaan Valley of Tucker County. Ten natural history morphometrics and characters were …

Dopamine Modulates The Lateral Giant Neuron And Serotonergic Facilitation In Crayfish, Joshua Scott Titlow

Theses, Dissertations and Capstones

The lateral giant (LG) neural circuit is a model system used to study the function of individual neurons. The LG circuit is part of a tail flip escape reflex that is a defensive behavior for crayfish. This thesis begins by addressing the effects of dopamine (DA), a neurotransmitter involved in normal and abnormal behaviors of most animals. Here it is shown that dopamine decreases the excitability of the LG neuron, a trigger for the escape reflex. An electrophysiology protocol was used to mimic sensory input to the LG neuron. Stimulating a sensory nerve in the last ganglion with an electrode …

Determining The Distribution Of Antibiotic Resistant And Fecal Indicator Bacteria In The Ohio River, Caitlin Nicole Swecker

Theses, Dissertations and Capstones

The Ohio River extends 981 miles from Pittsburgh, PA to Cairo, Ill providing drinking water to over three million people, a natural habitat for aquatic life, a public recreation resource, a major transportation route, and a source of water for industry. The Guyandotte River is a highly impacted tributary emptying into the Ohio River in Huntington, WV. The objectives of this study were to determine if sediment load is correlated to the number of antibiotic resistant bacteria (ARB) and determine if a single surface sample is a sufficient representative measurement of ARB populations in a large river. In 2007, subsurface …