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Articles 121 - 150 of 342
Full-Text Articles in Ecology and Evolutionary Biology
Regenerative Agriculture Effects On Invertebrate And Bird Communities And Insect-Provided Ecosystem Services, Alex Michels
Regenerative Agriculture Effects On Invertebrate And Bird Communities And Insect-Provided Ecosystem Services, Alex Michels
Electronic Theses and Dissertations
Conventional agricultural practices can have unintended consequences on the environment and wildlife. Insects and birds are declining at rapid rates around the world, and the current conventional agricultural paradigm is a major driver through habitat loss and the intensification of production. Invertebrates in agroecosystems provide services to both farmers and the rest of society. Regenerative systems may promote the functioning of an agroecosystem by influencing invertebrate abundance, diversity, and ecosystem services and mitigate bird and insect declines through conservation practices that increase soil health, reduce disturbances, and increase biological diversity. Here I address knowledge gaps of the effects of regenerative …
Eastern Redcedar (Juniperus Virginiana L.) Encroachment On South-Central South Dakota Rangelands: Impact On Plant Communities, Austin K. Domeier
Eastern Redcedar (Juniperus Virginiana L.) Encroachment On South-Central South Dakota Rangelands: Impact On Plant Communities, Austin K. Domeier
Electronic Theses and Dissertations
Grasslands historically covered 46 million km2 of Earth’s surface representing nearly 42% of the living vegetation. Encroachment of woody species onto grasslands and savannas is a widely researched global phenomenon, with eastern redcedar (ERC) (Juniperus virginiana L.) trees being the most prominent woody encroacher in the Northern Great Plains (NGP) due to the lack of prescribed fire and planted ERC escaping from shelterbelts. This encroachment poses a threat to native plant communities in terms of their reproduction, regeneration, and diversity. ERC are fierce competitors and can establish in most soil types, are drought tolerant, and prolific seed reproducers. These qualities …
Living In A Gradient: The Influence Of Water Temperature Variation On Development, Settling Time And Survival Of Pallid Sturgeon Larvae In The Missouri River, Maria Elizabeth Erceg
Living In A Gradient: The Influence Of Water Temperature Variation On Development, Settling Time And Survival Of Pallid Sturgeon Larvae In The Missouri River, Maria Elizabeth Erceg
Electronic Theses and Dissertations
Pallid Sturgeon (Scaphirhynchus albus) are a federally endangered species experiencing widespread reproduction and recruitment failures. Recruitment failure is hypothesized to be caused by habitat modifications made to the Missouri River that disrupt connectivity and alter temperature profiles downstream of constructed dams. Hypolimnetic releases from Missouri River dams affect the temperature downstream, creating colder conditions during the downstream drifting phase for dispersing larvae. Understanding the influence of water temperature changes on larval development is crucial for recovery efforts. In this study, we evaluated the effects of water temperature daily heating rate on energy use, settling behavior, and growth rate of endogenously …
Factors Influencing Mortality Of Stocked Rainbow Trout In Black Hills Reservoirs, Charles A. Mordhorst
Factors Influencing Mortality Of Stocked Rainbow Trout In Black Hills Reservoirs, Charles A. Mordhorst
Electronic Theses and Dissertations
Because return to angler is an important outcome of put-and-take fisheries, understanding mortality of stocked Rainbow Trout is fundamental to managing these fisheries. Harvest rates of stocked Rainbow Trout Oncorhynchus mykiss in the Black Hills of South Dakota are believed to be below management objectives. Rainbow Trout not harvested by anglers are assumed to be lost to various sources of mortality, raising concerns about the cost of the Rainbow Trout stocking program relative to the benefit provided to anglers. (Simpson 2008). This study evaluated the factors influencing mortality of Rainbow Trout stocked into Black Hills reservoirs. We assessed the effects …
Evaluating Avian Use Of Cover Crops In The Corn Belt, Megan Figura
Evaluating Avian Use Of Cover Crops In The Corn Belt, Megan Figura
Electronic Theses and Dissertations
The tallgrass prairie of North America has changed drastically since colonial settlement, with up to 99% of this region converted for agriculture and other land-uses. Concurrent with grassland conversion, grassland birds have experienced the most extreme, consistent, and widespread population declines of any avian guild. Agricultural lands in the U.S. Midwest were able to provide adequate habitat for several bird species until the 1950’s; however, altered and intensified management practices have degraded much of remaining suitable habitat and undermined ecosystem functions. Consequently, many grassland birds have been identified as Species of Greatest Conservation Need (SGCN) in many State Wildlife Action …
Targeted Browsing With Goats For Eastern Redcedar (Juniperus Virginiana L.) Control, Alanna M. Hartsfield
Targeted Browsing With Goats For Eastern Redcedar (Juniperus Virginiana L.) Control, Alanna M. Hartsfield
Electronic Theses and Dissertations
As eastern redcedar (ERC) (Juniperus virginiana L.) grassland encroachment progresses, all potential control methods should be explored in the interest of Great Plains grassland health and longevity. Targeted browsing with goats has been proven as an effective control method on some juniper species; however, little is known about its ability to control ERC. These studies intend to mend knowledge gaps of how targeted browsing with goats control ERC by causing tree death without chemicals or machinery. The first study is two 3x3 Latin squares comparing protein-supplemented diets. The second study is a randomized complete block design of five 0.224 ha …
A Post-Pneumonia Epizootic Evaluation Of The Rapid City, South Dakota Bighorn Sheep Herd, Amanda N. Ensrud
A Post-Pneumonia Epizootic Evaluation Of The Rapid City, South Dakota Bighorn Sheep Herd, Amanda N. Ensrud
Electronic Theses and Dissertations
Pneumonia is a major factor affecting populations of free-ranging bighorn sheep (Ovis canadensis) across western North America. Pneumonia can occur in large-scale epizootics, during which greater than half of the population typically dies. After these epizootics, surviving ewes continue to conceive and bear lambs. However, lamb recruitment may remain low due to periodic or annual pneumonia outbreaks causing high lamb mortality rates, sometimes greater than 90%. Our study focused on the Rapid City, South Dakota bighorn sheep (BHS) herd that has recorded pneumonia-induced population decline since 2009. The first objective was to improve lamb health and survival by identifying and …
Post-Fledging Habitat Selection And Movements Of Juvenile Mallards In The Prairie Pothole Region, Cynthia E. Anchor
Post-Fledging Habitat Selection And Movements Of Juvenile Mallards In The Prairie Pothole Region, Cynthia E. Anchor
Electronic Theses and Dissertations
The Prairie Pothole Region (PPR) of North America provides critical habitat for waterfowl across life stages, but anthropogenic changes to the landscape have negatively impacted habitat quality for waterfowl. The mallard (Anas platyrhynchos) is abundant and socioeconomically valuable in the PPR, as it is a preferred target species of waterfowl hunters. Extensive research has evaluated breeding ecology of mallards in the PPR, but the period between juvenile mallards fledging and migrating has rarely been studied. The post-fledging ecology of juvenile mallards is a vital consideration for comprehensive waterfowl management. Further, juvenile mallards are a significant portion of hunter harvest at …
Distinguishing Mustela From Neogale (Mustelidae) Through Both A Qualitative And Quantitative Analysis Of Skull And Tooth Morphology, Ronald W. Peery
Distinguishing Mustela From Neogale (Mustelidae) Through Both A Qualitative And Quantitative Analysis Of Skull And Tooth Morphology, Ronald W. Peery
Electronic Theses and Dissertations
Weasels and mink (Mustela and Neogale) can be difficult to distinguish osteologically due to similarities in morphology, thus suggesting the need for an accurate tool in distinguishing among taxa. This study utilized a combination of character state and stepwise discriminant function (DFA) analyses to examine potential distinguishing features of skull and tooth morphology. Measurements and ratios were collected from all 18 extant musteline species, as well as the extinct Neovison macrodon, Mustela rexroadensis, Mustela meltoni, Mustela gazini, and Mustela jacksoni. Unidentified musteline specimens from the Gray Fossil Site were also included. Results of …
Lipidomic Analysis Of Various Developmental Stages Of Physcomitrium Patens, Deepshila Gautam
Lipidomic Analysis Of Various Developmental Stages Of Physcomitrium Patens, Deepshila Gautam
Electronic Theses and Dissertations
Lipids maintain fluidity of the cell membrane during the lifetime of all organisms. The moss Physcomitrium patens, an early land plant, enters reproductive phase under cold (15°C) conditions relative to its gametophytes (22°C). Thus, we hypothesized that their lipid content and composition would be distinct. Using ESI-MS/MS, we showed that the content and acyl composition of 11 lipid classes varied during development. Galactolipids were abundant in gametophytes but insignificant in sporophytes; among phospholipids, phosphatidylcholine was predominant in both phases. Although, sporophytes contained around five-fold less lipids than the gametophyte, their phosphatidic acid content, which accumulates during stress, was 18-fold …
Natural Regeneration Dynamics And Survival Influenced By Abiotic And Biotic Factors In A Bottomland Hardwood Forest, Lydia J. Voth Rurup
Natural Regeneration Dynamics And Survival Influenced By Abiotic And Biotic Factors In A Bottomland Hardwood Forest, Lydia J. Voth Rurup
Electronic Theses and Dissertations
Riparian ecosystems are vital to the landscape, providing critical services including water filtration and purification, flood and erosion control, carbon sequestration, biodiversity support, and aesthetic value. Bottomland hardwood forests, however, are threatened by invasive species, land loss/conversion, inconsistent or absence of harvesting disturbances, and altered hydrological patterns, leading to reduced success of desired, native species. This research assessed regeneration dynamics and one-year survival in a seasonally-flooded bottomland hardwood forest at Boggy Slough Conservation Area in East Texas to identify abiotic and biotic factors important for successful establishment of native regeneration. Areas sampled included two that were previously treated with herbicide …
How Do Cover Crops Change Soil Health In A No-Till System?, Aysha Kirsten Tapp Ross
How Do Cover Crops Change Soil Health In A No-Till System?, Aysha Kirsten Tapp Ross
Electronic Theses and Dissertations
Nutrient levels and aggregation measurements are currently the most accurate means to measure soil health. It has been suggested that bacterial and fungal communities may prove to be a more accurate measure of soil health. In this study soil microbe communities and nutrient levels were compared in rye cover cropped soils to measure for differences between treatments. Effects between the microbial communities and environmental measurements were also measured within those treatments to test for correlations between soil health measures and microbial communities. The plots were put in a biennial corn (Zea mays L.) and soybean (Glycine max …
Using Predator Vocalizations To Deter Raccoon Predation On Nests Of Diamondback Terrapins (Malaclemys Terrapin), Colleen Elizabeth Naeger
Using Predator Vocalizations To Deter Raccoon Predation On Nests Of Diamondback Terrapins (Malaclemys Terrapin), Colleen Elizabeth Naeger
Electronic Theses and Dissertations
No abstract provided.
Community Structure And Dynamics Of Benthic Macroinvertebrates In A Recreated Headwater Stream System On A Valley Fill In A Retrofitted Watershed Located In The Appalachian Coalfields Of Southeastern Kentucky (U.S.A.), Steven W. Bailey
Electronic Theses and Dissertations
The extraction of coal from steep-gradient surface mining sites such as in the Appalachian Coalfields of the U.S. produces excess debris that is often placed in adjacent valleys resulting in the creation of valley fills. Not only are headwater streams buried in the process, but watershed functions are either destroyed outright, or become fragmented and disconnected from adjacent ecosystems resulting in adverse effects to downstream biological communities. In this dissertation, the dynamics of stream macroinvertebrate community structure, composition, diversity, and biotic integrity are assessed at a “proof of concept” stream system recreated on a retrofitted valley fill. For comparison, two …
Food-Web And Functional Trait Community Structure Of Predator Assemblages In Pine Forests Under Different Management Regimes, Connor Adams
Food-Web And Functional Trait Community Structure Of Predator Assemblages In Pine Forests Under Different Management Regimes, Connor Adams
Electronic Theses and Dissertations
Anthropogenic activities can alter natural disturbance regimes in ecosystems, and thereby affect the structure and function of biological diversity. As many of the world’s ecosystems are degraded beyond natural recovery, well-defined restoration goals are necessary to maintain the ecological processes that provide valuable ecosystem services. Utilizing taxonomic, functional, and food-web approaches, I investigated the impacts of forest management practices in structuring predator communities in two pine forest systems of eastern Texas. The results of this study indicate that the increased frequency of forest management practices such as prescribed fires and thinning operations encourages predator diversity while increasing functional and trophic …
Characterizing The Dormancy And Repair Of A Circumneutral Passive Remediation System Receiving Iron And Sulfate-Rich Amd, Garrett Struble
Characterizing The Dormancy And Repair Of A Circumneutral Passive Remediation System Receiving Iron And Sulfate-Rich Amd, Garrett Struble
Electronic Theses and Dissertations
The Wingfield Pines remediation system is a ~20-acre circumneutral, gravity-fed system constructed in 2009 to passively remediate Fe from an ~2000 gallon/minute effluent. In 2017, a fracture to the system’s underground cavern caused the AMD effluent to bypass the system, flowing directly into Chartiers Creek and leaving the system decommissioned between 2017-2019. Soil slurry and water samples were collected in the weeks prior to the system’s reinstalment in September 2019 through January 2020 in order to determine the effect of the repair on remediation efficiency and microbial community composition. This study acted as the first to describe the temporary decommissioning …
Saving Butterflies In The City: Gardens As Conservation Spaces In Urban Landscapes., Lindsay D. Nason
Saving Butterflies In The City: Gardens As Conservation Spaces In Urban Landscapes., Lindsay D. Nason
Electronic Theses and Dissertations
Butterfly populations are declining, and habitat degradation due to urbanization is a major contributing factor. Gardens represent a large proportion of land area in many cities, and thus may be important for conserving butterflies in urban environments. In this dissertation, I examine how garden features (ex: size, level of urbanization, plant diversity) affect adult butterfly diversity/abundance, behavior, and the predation risk faced by caterpillars. My study sites were native plant gardens in Jefferson, Bullitt, and Hardin Cos.,KY. In Chapter One, I used clay caterpillars to assess differences in predation pressure in gardens along a gradient of percent impervious surface (%IS). …
Not All Pollinator Gardens Are Created Equally: Determining Factors Pertinent To Improving Pollinator Garden Effectiveness, Travis Watson
Not All Pollinator Gardens Are Created Equally: Determining Factors Pertinent To Improving Pollinator Garden Effectiveness, Travis Watson
Electronic Theses and Dissertations
Increasing evidence documenting the decline of insect populations, resulting from increasing human disturbances has resulted in efforts to establish pollinator gardens to provide additional resources for insect populations. However, our understanding of biotic and abiotic garden characteristics important for attracting and sustaining pollinator diversity is limited. Here, we evaluated 17 pollinator gardens to evaluate the effect of five biotic and three abiotic garden characteristics on pollinator species richness, abundance, and proportional representation of four pollinator functional groups. Plant species richness positively influenced pollinator richness and negatively influenced flower visitation. Bombus proportional abundance responded to several variables (distance to vegetation, plant …
Effects Of Respiratory Perturbations On Aging And Healthspan In Daphnia Magna, Millicent Nkiruka Ekwudo
Effects Of Respiratory Perturbations On Aging And Healthspan In Daphnia Magna, Millicent Nkiruka Ekwudo
Electronic Theses and Dissertations
Aging is a degenerative process characterized by a decline in physiological functions and cellular activities. Environmental and pharmacological interventions affecting longevity pathways have been extensively studied in model organisms. This study investigated the effect of chronic mild intermittent hypoxia (4 mg O2/L) or mild mitochondrial uncoupling with three doses of 0 (control), 0.1, 1, and 5 μM of 2,4-Dinitrophenol (DNP), on life history and gene expression in four clones of Daphnia magna. Interestingly, clones from intermittent ponds displayed better tolerance to hypoxia and DNP. Although neither treatments extended longevity, hypoxia increased fecundity and body size, and decreased food consumption and …
Effects Of Anthropogenic Light And Noise On Anuran Breeding Behavior, Ashley Kobisk
Effects Of Anthropogenic Light And Noise On Anuran Breeding Behavior, Ashley Kobisk
Electronic Theses and Dissertations
The natural environment can be negatively impacted by a variety of human activities, including the production of artificial light at night and anthropogenic noise. Recent studies suggest that pollution from anthropogenic light and noise alters animal behavior. Despite being highly nocturnal and vocal animals, little attention has been given to anurans and the effects artificial light at night and anthropogenic noise have on their behavior. This study investigated the effects of artificial light at night and anthropogenic noise on anuran breeding systems in eastern Texas. Specifically, this study investigated whether (1) artificial light and anthropogenic noise altered calling behavior in …
Distribution And Ecological Effects Of Azteca Chartifex/Trigona In The Barro Colorado Nature Monument., Rachel Leann Wells
Distribution And Ecological Effects Of Azteca Chartifex/Trigona In The Barro Colorado Nature Monument., Rachel Leann Wells
Electronic Theses and Dissertations
This dissertation explores the behavior and ecology of a conspicuous and behaviorally dominant ant species in the tropical rainforest of Panama. Competition with aggressive species is an important factor that shapes local community structure. Eavesdropping on the chemical communication systems of such behaviorally dominant species can help heterospecific species to avoid negative interactions. I review the chemical communication system and known ecological effects of the Neotropical canopy ant, Azteca chartifex/trigona (Chapter 1). There are over 40 known compounds produced by Azteca workers, yet how these compounds impact heterospecific species is unclear in many circumstances. I assessed if heterospecific …
The Bees Of Two Sites In The Big Thicket National Preserve, Texas, With A Consideration Of The Effects Of A Rare Flooding Event, Archie Ray Sauls Jr.
The Bees Of Two Sites In The Big Thicket National Preserve, Texas, With A Consideration Of The Effects Of A Rare Flooding Event, Archie Ray Sauls Jr.
Electronic Theses and Dissertations
Native bees are an important part of terrestrial ecosystems due to their coevolution with flowering plants. This study catalogued the bee fauna of two sandyland sites in the Big Thicket National Preserve and assessed whether a community was impacted by a historic hurricane-induced flooding event. It was hypothesized that a change in diversity metrics would be evident following the flood. Datasets were analyzed for differences in species richness, abundance, evenness, and Shannon’s diversity. Similarities between datasets were also assessed using the multivariate tests analysis of similarity (ANOSIM) and similarity of percentages (SIMPER). At two sites over two years of sampling …
The Ecological Resilience Of A Tropical Montane Pine Forest To Droughts, Fire, And Hurricane Disturbance, Daniel Erland Brugger Swann
The Ecological Resilience Of A Tropical Montane Pine Forest To Droughts, Fire, And Hurricane Disturbance, Daniel Erland Brugger Swann
Electronic Theses and Dissertations
Owing to the geographic isolation and unique climatic conditions which occur in tropical montane forests (TMFs), these systems are highly sensitive to changes in both climate and disturbance. Therefore, as ongoing climate change alters disturbance regimes – potentially facilitating multiple disturbance interactions much greater than that of isolated disturbances – TMFs may be shifted to alternate stable states. However, study of TMF resilience is essentially unknown given the lack of long-term studies in these systems. We address the resiliency of the tropical montane pine forests of the Cordillera Central, Dominican Republic using long-term climate records, remeasured vegetation data, and reconstructed …
Understanding Patterns And Functional Impacts Of An Invasive Tree And Its Biological Control In A Riparian System, Annie L. Henry
Understanding Patterns And Functional Impacts Of An Invasive Tree And Its Biological Control In A Riparian System, Annie L. Henry
Electronic Theses and Dissertations
Invasive species have become an inextricable part of the landscape, particularly in riparian plant communities, and removal is often a key component of restoration programs. Biological control (biocontrol) is a method of removal that is often both efficient and effective. However, the impact of biocontrol on target species and indirect effects from invasive species removal can be hard to predict. While monitoring the impact of invasive species removal usually involves some species-based assessment such as changes in diversity, historically dominant species or native species, these strategies do not typically provide insight into the mechanisms underlying plant community response to removal. …
Pollinator Ecology In Agroecosystems Of Eastern South Dakota, Isabela Beatriz Vilella-Arnizaut
Pollinator Ecology In Agroecosystems Of Eastern South Dakota, Isabela Beatriz Vilella-Arnizaut
Electronic Theses and Dissertations
Community structure contributes to ecosystem persistence and stability. To understand the mechanisms underlying pollination and community stability of natural areas in a human influenced landscape, a better understanding of the interaction patterns between plants and pollinators in disturbed landscapes is needed. Furthermore, understanding the potential conservation value in green spaces such as botanical gardens could assist in preserving and bolstering plant-pollinator interactions. Our study takes place in the Northern Great Plains which still retains extensive tracts of remnant temperate grassland habitat within a matrix of varying land-uses. First, we used a network-based approach to quantify how temperate grassland attributes and …
Pheasant Ecology In An Agricultural Landscape Of South Dakota, Sprih Harsh
Pheasant Ecology In An Agricultural Landscape Of South Dakota, Sprih Harsh
Electronic Theses and Dissertations
Habitat loss and fragmentation are the greatest threats to wildlife conservation. Grasslands are among the most threatened ecosystems worldwide. The large-scale conversion of North American grasslands to cultivation has been strongly associated with declines of grassland bird populations. The ring-necked pheasant (Phasianus colchicus) is a common grassland bird which is negatively impacted by the conversion of grassland to cropland. Though pheasants are non-native to South Dakota, they have become naturalized in most of the state. However, with increases in agricultural intensification in South Dakota, indices of pheasant abundance from brood route surveys suggest that pheasant populations have declined to historically …
An Evaluation Of The Bighorn Sheep Population In Badlands National Park, Austin J. Wieseler
An Evaluation Of The Bighorn Sheep Population In Badlands National Park, Austin J. Wieseler
Electronic Theses and Dissertations
Within the last century, bighorn sheep (Ovis canadensis) in the badlands ecosystem of western South Dakota have been subjected to complete extirpation, reintroduction, disease die-offs, genetic bottlenecking, and population augmentation. Subsequently, the population in Badlands National Park (BNP) appears to have recovered, but it was unknown to what degree past events had influenced the population. From 2017-2019, we conducted research on 5 subherds within 2 management units in BNP to 1) survey for the presence of respiratory pathogens and estimate the prevalence of other potentially infectious diseases; 2) assess adult and lamb survival and cause-specific mortality; 3) estimate population size …
Spatial And Temporal Patterns Of Sympatric Bobcats (Lynx Rufus) And Coyotes (Canis Latrans) In An Agricultural Landscape, Marlin M. Dart
Spatial And Temporal Patterns Of Sympatric Bobcats (Lynx Rufus) And Coyotes (Canis Latrans) In An Agricultural Landscape, Marlin M. Dart
Electronic Theses and Dissertations
Bobcat (Lynx rufus) populations experienced declines in the Midwest during the 20th century due to land conversion for agriculture and over exploitation and were practically nonexistent in areas by the 1970-80s. Populations have been recovering following changes in land-use practices and habitat improvement. Eastern South Dakota was closed to bobcat harvest in 1977 but reopened in 2012 to select counties. Bobcats are elusive, have large home ranges, and occur at low densities, making monitoring their populations difficult. Camera trapping is an effective tool for monitoring elusive carnivores but can be burdened by low detection rates. Researchers often employ …
Revegetating Salt-Impacted Soils In The Northern Great Plains, Abigail P. Blanchard
Revegetating Salt-Impacted Soils In The Northern Great Plains, Abigail P. Blanchard
Electronic Theses and Dissertations
In the northern Great Plains (NGP), an estimated 10.6 million hectares of land are affected by naturally occurring salt-impacted soil. Naturally occurring salt impaction results when rainfall causes salts in parent material to move upward through the soil profile and remain in the root zone causing osmotic and ionic stress, negatively affecting seed imbibition, germination, and plant growth. Common methods to remediate saltimpacted soils were developed in the irrigated soils of the Southwestern U.S., are ineffective in the non-irrigated soils of the NGP, and can exacerbate the problem. Therefore, new methods to remediate salt-impacted soil in the NGP are needed. …
Effects Of Predator Management And Parasitism On Over-Water Nesting Diving Duck Production In Southwestern Manitoba, Trenton E. Rohrer
Effects Of Predator Management And Parasitism On Over-Water Nesting Diving Duck Production In Southwestern Manitoba, Trenton E. Rohrer
Electronic Theses and Dissertations
Nest survival of upland nesting waterfowl (e.g., mallard [Anas platyrhynchos], blue-winged teal [Anas discors]) has been studied extensively in the Prairie Pothole Region (PPR) of North America. Conversely, over-water nesting diving ducks’ nest survival is poorly understood, likely because over-water nests are more challenging to find, may be less concentrated, and searching for these nests is physically demanding. Nest depredation is considered a leading cause of duck nest failure and is considered a limiting factor on upland nesting waterfowl production in the PPR. Similar factors limiting upland nesting duck production may limit over-water nesting duck productivity, but studies of this …