Ecology and Evolutionary Biology Commons^™

Benthic Biodiversity And Physico-Chemical Parameters Of Acid Mine Drainage, Acid Impacted And Nonimpacted Streams, Stephen D. Greene

Theses, Dissertations and Capstones

South Fork of Sand Lick Creek, Logan County, West Virginia, drains an abandoned coal strip mine which had exploited Pottsville series coalbeds (Pennsylvania strata). These strata outcrop throughout southwestern West Virginia. North Fork watershed is relatively unchanged, save a small roadcut throughout. South Fork benthic community had not recovered although mining activity had ceased about 20 years earlier. Benthic communities were analyzed with detrended correspondence analysis (DCA). Family Chironomidae predominated South Fork benthic community throughout the study. North Fork’s benthic community had as major contributors acid resistant caddisfly family Hydropsychidae, mayfly family Baetidae, and stonefly families Perlodidae and Nemouridae. Family …

Results In The Development Of A Macroinvertebrate Index For The Ohio River, Matthew S. Wooten

Theses, Dissertations and Capstones

Aquatic macroinvertebrates are utilized widely as indicators of water quality. Many indices are in use today that provide a means of applying a numeric value to the water quality of a particular stream. However, these indices have been designed for use on small streams and creeks. The primary objective of this study was to lay a foundation for the development of a marcroinvertebrate index to be used on the Ohio River. Secondarily, this study examined for longitudinal trends, trends in correlation with effluent location and the determination of least impacted or reference conditions on the Ohio River. Regression correlation analysis …

Influences Of Relative Humidity, Soil And Air Temperatures, And Lunar Phase On Occurrence Of Terrestrial Plethodontid Salamanders At High Elevation Sites, Jo Santiago

Theses, Dissertations and Capstones

Microhabitat environments and occurrences of terrestrial plethodontid salamanders including Plethodon nettingi Green, Plethodon cinereus (Green), Plethodon wehrlei Fowler and Dunn, Plethodon glutinosus (Green), and Desmognathus ochrophaeus Cope were monitored over a 15 month period at high elevation sites in the Stuart Knob area of the Monongahela National Forest in eastern West Virginia. Environmental data collected for eight equal-sized study plots (8m² ) included relative humidity, air and soil temperatures, and lunar phase. Five hundred and twenty-five salamanders were captured in diurnal and nocturnal searches, and an additional 52 individuals were observed. It was found that the most common occurring …

Reproductive Biology, Food Habits, And Forage Ratios Of The Candy Darter, Etheostoma Osburni, In The Cherry River, West Virginia, With An Analysis Of Mitochondrial Dna, Ann Elizabeth Schoolcraft

Theses, Dissertations and Capstones

Etheostoma osburni, the candy darter, is endemic to the lower New River drainage of West Virginia and Virginia and Gauley River drainage of West Virginia. As a result of its endemism and evidence of declining population numbers, the species has been given the status of Special Concern in both states. Candy darters occur in the Appalachian Plateau and Ridge and Valley provinces, and are influenced by different environmental selective pressures. In this study, candy darters were collected in the summer and fall of 1998 and spring of 1999 by backpack electroshocking for determination of their seasonal reproductive biology, food …

Ecology And Natural History Of The Cow Knob Salamander, Plethodon Punctatus Highton, In West Virginia, Robert B. Tucker

Theses, Dissertations and Capstones

Very little has been written on the ecology and natural history of the Cow Knob salamander, Plethodon punctatus Highton, since it was described by Highton (1971). Plethodon punctatus is known only from the higher elevations of Great North (above 2,800 ft) and Shenandoah (above 3,000 ft.) Mountains in Virginia and West Virginia (Highton, 1972; Conant and Collins, 1991). The type locality is Cow Knob, Pendleton County, West Virginia. Plethodon punctatus is a large (up to 75 mm snout-to-vent length) dorso-ventrally flattened salamander with creamish iridiophore spots on the back and sides, webbed feet for a fossorial lifestyle, and large …

Life History, Ecology, And Low Ph Tolerance Of The Freshwater Prawn, Palaemonetes Kadiakensis Rathbun, In A Mitigated Wetland Of The Green Bottom Wildlife Management Area, West Virginia, Jennifer Ann Aderman

Theses, Dissertations and Capstones

An ecological life history study of the freshwater prawn Palaemonetes kadiakensis was conducted at the mitigated wetland of the Green Bottom Wildlife management Area (GBWMA). Monthly samples were collected from November 1996 through November 1997. In addition, weekly samples were collected from May through November for growth and reproductive analysis. Monthly samples yielded 520 prawns. Palaemonetes kadiakensis had a one-year life cycle, with all adults dying by September. Males averaged 23.82 mm (range = 14.15 to 36.75 mm) and females averaged 26.78mm (range = 15.06 to 38.60 mm) in length. Males averaged 0.087g (range = 0.014to 0.293 g) and females …

An Assessment Of The Prevalence Of The Bass Tapeworm, Proteocephalus Ambloplitis, And Other Parasites In Five Species Of Basses From River And Lake Ecosystems In West Virginia, J. Alyce Bradbury

Theses, Dissertations and Capstones

Two-hundred and fifteen basses were collected from four river sites (Racine Locks and Dam, Robert C. Byrd Locks and Dam; Ashland, KY—Ohio River; London Locks and Dam—Kanawha River) and five reservoir sites (Beech Fork Lake, East Lynn Lake, Summersville Lake, Burnsville Lake, and Stonewall Jackson Lake,). The five species of bass caught were: largemouth bass, Micropterus salmoides; smallmouth bass, Micropterus dolomieui; spotted bass, Micropterus punctulatus; white bass, Morone chrysops; and hybrid bass, Morone chyrsops x Morone saxatilis. Basses were collected by three methods: 1) local anglers (hook and line), 2) gill netting surveys; and 3) …

The Use Of Emergent Rocks As Refugia For The Cheat Mountain Salamander, Plethodon Nettingi Green, Beth Anne Pauley

Theses, Dissertations and Capstones

Plethodon nettinqi was listed as a threatened species in 1989 by the United States Fish and Wildlife Service (Pauley, 1991). Its total range is within 5 counties in eastern West Virginia. There are fewer then 60 disjunct populations known and most populations are above 3,000 ft. and are associated with emergent rocks or narrow ravines with Rhododendron. It is hypothesized the P. netting! survived lumbering practices at the turn of the century by taking refuge beneath large emergent rocks and narrow ravines with Rhododendron. This study examined environmental factors associated with emergent rocks that might regulate the distribution of P. …

The Use Of Isoelectric Focusing To Assess The Genetic Diversity Of The Cyprinid Fishes: Rhinichthys Cataractae And Rhinichthys Atratulus In West Virginia, Fauzia Alam Miah

Theses, Dissertations and Capstones

In this study, isoelectric focusing is used to separate proteins of Rhinichthys cataractae and R. atratulus and conventional staining is used to identify alleles of six genes. The objective of this work is to determine whether these methods can effectively assay isozymes that may be used to establish the relationship between anthropogenic stress and genetic diversity. Alleles for LDH-A, LDH-B, EST-A, EST-B, EST-C, and EST-D loci were identified for two species of fish, R. atratulus and R. cataractae. Both fish species are known to be sensitive to anthropogenic stress and known to be widely distributed in stressed environments. Alleles …

Ecological Life History Of Caenis Amica Hagen (Emphemeroptera: Caenidae) From The Mitigated Area Of Green Bottom Wildlife Management Area, Cabell County, West Virginia, Gail Lynn Perrine

Theses, Dissertations and Capstones

The ecological life history of a population of Caenis amica Hagen from the Green Bottom Wildlife Management Area was studied from November 1996 to November 1997. Water quality data and 2,505 larvae were collected from the newly mitigated area of Green Bottom Swamp. Total body lengths of larvae were measured using a computer-digitizing program. These results indicated a bivoltine life cycle. Most larvae were found in the spring and summer months of April, June, and July. The highest peak of larvae (1,035) occurred in July. The pH values ranged from 7.0 to 8.0 with a mean of 7.6. Temperatures ranged …

Natural History Of The Four-Toed Salamander, Hemidactylium Scutatum, In West Virginia, Sandra L. Kilpatrick

Theses, Dissertations and Capstones

A 2-year study was conducted to determine the reproductive and nesting habits, embryonic and larval development, and tolerance to acid conditions of Hemidactylium scutatum in West Virginia. Five study sites located in or adjacent to the Otter Creek National Wilderness Area, Monongahela National Forest, Randolph County, West Virginia, were monitored to determine nesting habits and length of incubation and larval periods in Hemidactylium Time of breeding was determined by spermatogenic wave analysis and time of egg deposition was determined by examination of ovarian follicles and field observations of gravid females migrating to nest sites. Breeding occurred in autumn and again …

Ecological Life History Of Crangonyx Pseudogracilis Bousfield (Crustacea: Amphipoda) In The Green Bottom Wildlife Management Area, Cabell County, West Virginia, Andrea Leigh Henry

Theses, Dissertations and Capstones

Although the genus Crangonyx has been studied in the Appalachian area, most of this work has concentrated on subterranean species. A Crangonyx pseudogracilis population from the Green Bottom Wildlife Management Area (GBWMA), Cabell County, West Virginia was sampled weekly from March 1995 through July 1996 to investigate the ecological life history of the amphipod. A total of 1508 amphipods were collected. Amphipods were present in the study sites from November through July, at which time they migrated to deeper waters. Crangonyx pseudogracilis had a one year life cycle and bred from November to May. Adult amphipods began to die off …

An Ecological Study Of Shale Barren Rock Cress (Arabis Serotina Steele) At Little Fork Shale Barren, Pendleton County, West Virginia, Robert J. Jarrett

Theses, Dissertations and Capstones

Shale Barren Rock Cress (Arabis serotina Steele) was listed as a federally endangered species by the U.S. Fish and Wildlife Service in August 1989. As part of a cooperative agreement between the West Virginia Natural Heritage Program and the U.S. Navy, research was conducted in 1994 and 1995 at Little Fork Shale Barren (LFSB) to provide baseline information on the population dynamics of A. serotina and to study the vegetation and physical parameters associated with this shale barren community. Soil samples and plant community data were collected from 40 1-m² circular plots for herbaceous vegetation and six 0.02-ha …

Natural History Of The Red-Spotted Newt, Notophthalmus Viridescens Viridescens (Rafinesque), In West Virginia, Jennifer M. Piascik

Theses, Dissertations and Capstones

Many studies have been conducted on Notophthalmus viridescens viridescens but none have occurred in West Virginia. There are variations in the life history of newts depending on location. In this study, I studied a population of newts in Wayne County, West Virginia from February 1996 to June 1997. I examined population structure, seasonal activity, and reproductive biology.

The population structure can be divided into four classes: egg, larvae, eft, and adult. Eggs at Shoals were deposited in mid-May and larvae were present from mid-June to early September. Larvae began to transform at an approximate total length of 32.8 mm. Red …

Observations On Reproduction, Isozyme Patterns, And Meristics In Mosquitofish (Pisces: Poeciliidae) From West Virginia, Matt Mcreynolds

Theses, Dissertations and Capstones

Prior to 1988, two subspecies of the mosquitofish Gambusia affinis (G. a. affinis and G. a. holbrooki) were recognized along the Gulf and Atlantic Coasts of the United States. Based on electrophoretic analysis, subsequent investigators recognized G. holbrooki and G. affinis as separate species. Populations in drainages west of Mobile Bay were considered G. affinis, and those east of this divide being G. holbrooki. The only mosquitofish population in West Virginia is found in the Meadow River wetlands (1392 ha). This wetland complex is located at the western end of Greenbrier County in the Allegheny Mountain Province. …

Diet And Growth Of Larval And Juvenile Grass Pickerel, Esox Americanus Vermiculatus, And Central Mudminnow, Umbra Limi, In The Green Bottom Wildlife Management Area, Cabell County, West Virginia, Erica Midkiff

Theses, Dissertations and Capstones

The grass pickerel, Esox americanus vermiculatus, is listed as Undetermined on the Vertebrate Species List of Concern in West Virginia. The central mudminnow, Umbra limi, is a disjunct population in Green Bottom Swamp. The lentic, vegetated areas that are required for spawning for the grass pickerel and the central mudminnow have been reduced by residential, agricultural, and industrial developments. Green Bottom Swamp, a naturally occurring wetland of 58 ha, and a nearby mitigated wetland of 29 ha, provides spawning habitat for the grass pickerel and the mudminnow. Fishes were collected in beds of Potamogetan crispus and Ceratophyllum demursum …

A Taxonomic Study Of The Class Insecta In The Green Bottom Wildlife Management Area With Notes On The Life History Of Belostoma Lutarium (Stal), And Hesperocorixa Lucida (Abbott), Cabell County, West Virginia, Eric Wilhelm

Theses, Dissertations and Capstones

Green Bottom Wildlife Management Area (GBWMA), Cabell County, West Virginia, is a wetlands system adjacent to the Ohio River composed of wetlands varying in age from 4 to 60 years. This system is annually flooded by the Ohio River, yet insect communities remain unique to each vegetation type or microhabitat. Nine vegetative communities from two wetlands were sampled and compared using chi-square analysis to determine if the insect communities were unique. A total of 34,975 specimens, representing 23 families, were collected over ten sample intervals. Chi-square analysis on seven families common to all sites indicated that each vegetative community yielded …

Natural History, Ecology And Potential Environmental Stress Of ̲A̲M̲B̲Y̲S̲T̲O̲M̲A ̲M̲A̲C̲U̲L̲A̲T̲U̲M (Le Conte) And ̲R̲A̲N̲A ̲S̲Y̲L̲V̲A̲T̲I̲C̲A (Shaw) At Dolly Sods, West Virginia, Deborah L. Wegmann

Theses, Dissertations and Capstones

Studies were conducted on the natural history, ecology and potential stress of Ambystoma maculatum and Rana sylvatica at Dolly Sods, West Virginia. Temperature was found to be the primary stimulus influencing spring breeding migration of both species. Breeding period of R. sylvatica was found to be short in duration, resulting in deposition of eggs in large communal masses. Migration and breeding period of A. maculatum occurred from early-April to early-May. Egg deposition occurred from mid-April to mid-May. A second egg deposition of A. maculatum occurred mid-June. Temperature was found to have a significant influence on the rate of development in …

A Study On The Life History And Seasonal Foraging Habits Of The Salamander Desmognathus Quadramaculatus Holbrook, In Wv, Glenn R. Mills

Theses, Dissertations and Capstones

A study was conducted on Desmognathus quadramaculatus in the northern periphery of its range with some aspects of its life history, surface density, and seasonal foraging habits. The results in this study are compared to studies done on D. quadramaculatus in the southern portion of its range. Female D. quadramaculatus have been found tending egg clutches from June to September in the southern portion of its range and data collected in this study shows that egg deposition also occurs during this period in the northern population. Female size (SVL) at which D. quadramaculatus reach sexual maturity ranged from 57.6- 77.5 …

Nitrogen Transformations In A Mitigated Wetland In The Green Bottom Wildlife Management Area, Mark Allen Fisher

Theses, Dissertations and Capstones

Soil nitrogen (N) dynamics have been shown to be quite sensitive to the soil hydrology. Creation of a mitigation freshwater wetland from an old field provided an opportunity to examine changes in N dynamics in terrestrial, aquatic (but formerly terrestrial), and transition (seasonally inundated) soils.

This thesis determined N availability using in situ incubations of soils taken from three distinct habitat conditions based on the degree of inundation following wetland creation: (1) old field (no inundation), (2) transition (seasonally inundated, initially beneath 10-20 cm of water), and (3) mitigation wetland (permanently inundated). Sample plots were located along five parallel transects …

Geographic Variation Of S̲O̲R̲E̲X̲ C̲I̲N̲E̲R̲E̲U̲S̲ In West Virginia, Lisa Jane Gatens

Theses, Dissertations and Capstones

The morphological and geographical diversity of Sorex cinereus is evident in West Virginia. In an attempt to identify patterns of morphological variation relative to age, sex, and geographic locality, and to clarify taxonomic status of the West Virginia soricids by defining diagnostic characters applicable to this area, a sample of 288 specimens representing three taxa were analyzed statistically. Standard external measurements were recorded, and a series of 12 cranial and dental characters were measured and recorded for each specimen. Morphological variation due to age was found in S. c. cinereus, but not in the smaller reference samples of S. …

An Analysis Of Habitat Utilization And Feeding Ecology Of Plethodon Richmondi And Plethodon Cinereus In Northern West Virginia, Peter A. Kramer

Theses, Dissertations and Capstones

Studies were conducted on the comparative microhabitat requirements and feeling habits of the ravine salamander, Plethodon richmondi and the redback salamander, P. cinereus in northern West Virginia. Plethodon richmondi had a mean SVL of 49.7 ± 7.1 mm and were significantly larger than P. cinereus which had a mean SVL of 37.2 ± 8.0 mm. Both species had biennial egg laying patterns and deposited eggs in late spring or early summer. Juvenile P. richmondi were not surface active except for the early spring and neonates did not become surface active until the ensuing spring; conversely, P. cinereus juveniles exhibited more …

Genetic Analysis Of Rhinichthys Atratulus (Pisces: Cyprinidae) In North Central West Virginia, Alan Harper Tennant

Theses, Dissertations and Capstones

The blacknose dace, Rhinichthys atratulus, is a cyprinid minnow common to shallow streams throughout North America. It has been shown to be sensitive to anthropogenic stress and a study of the genetic nature of R. atratulus populations may yield information about their habitats. In West Virginia, two subspecies of R. atratulus are present, R. a. atratulus and R. a. obtusus, which are separated by the Allegheny divide. In north central West Virginia, R. a. atratulus was observed in the Cheat River drainage, an area previously thought be within the range of R. a. obtusus. Fish of the R. a. atratulus …

Patterns Of Soil Nitrogen Dynamics In A Central Appalachian Hardwood Forest Ecosystem, Bradley Michael Yurish

Theses, Dissertations and Capstones

Increasing awareness of the potential effects of acidic deposition prompted a watershed manipulation project at the Fernow Experimental Forest (FEF), West Virginia, in 1987. In this project, an acidifying agent, (Nf4)2S04 , was applied to an entire watershed to better understand ecosystem responses to simulated acid deposition in excess of ambient levels. In ecosystems receiving elevated N levels, increased soil N may contribute to soil acidification, lead to nutrient imbalances in plants, increase rates of nitrification, increase leaching of No3• and pollute surface waters. This study was designed to assess changes of three watersheds that received varied N inputs. The …

Cytogenetic And Isozyme Analysis Of Two Species Of North American Cyprinid Fishes : Rhinichthys Cataractae And Nocomis Micropogon From The Cheat River Drainages Of West Virginia, Syam B. Stoll

Theses, Dissertations and Capstones

Nocomos micropoqon and Rhinichthys cataractae are species of cyprinid fishes that may hybridize to produce a nominally described species Rhynichthys bowersi. Rhynichthys bowersi is a rare fish endemic to a few watersheds mostly in West Virginia, especially the Cheat River drainage. The resolution of its proper taxonomic status is presently a concern to state and federal regulatory agencies. A number of investigators have determined relatedness of cyprinid fishes by examining chromosomal structure. Telomeres and nucleolar organizer regions are chromosome structures used in inter and intraspecific phylogeny. In this study, fibroblasts from N. micropoqon and R. cataractae were first …

The Distribution, Ecology And Natural History Of Shrews (Insectivora: Soricidae) In Southern West Virginia, Jeffrey Jerome Hajenga

Theses, Dissertations and Capstones

A survey of the soricid (shrew) fauna of southern West Virginia was conducted between May 16, 1994 and May 27, 1995. Twenty-five sites were established in Mercer and Summers counties within seven habitat types. Pitfall traps were used and through a limitation of fluid depth within the trap the selectivity for soricids was increased to over 83% of the total capture. Overall, a total of 653 shrews comprising seven species (Sorex cinereus, S. /ongirostris, S. fumeus, S. dispar, S. hoyi, 8/arina brevicauda, and Cryptotis parva) was captured. Two of the species taken (S. hoyi and S. dispar) are new county …

Reproductive Biology Of The Grass Pickerel Esox Americanus Vermiculatus Lesueur, From The Green Bottom Wildlife Management Area, Cabell County, West Virginia, Ronald Clifton Tipton

Theses, Dissertations and Capstones

The grass pickerel, Esox american us vermiculatus LeSueur, is listed as Undetermined on the Vertebrate Species List of Concern in West Virginia. The reason for the West Virginia status is that lentic, vegetated habitats required for spawning by the grass pickerel are limited within the state and are being reduced by agricultural, residential, and industrial developments. Green Bottom Swamp, a naturally occurring wetland of 58 hectares, provides habitat for the grass pickerel. A proposed habitat alteration to add marshland by building dykes prompted this study. Information from this investigation will establish a baseline for reproductive activities of the grass pickerel …

Utilization Of Wood Duck Nest Boxes With Respect To Plant Community Type, Orientation And Juxtaposition At The Green Bottom Wildlife Management Area, West Virginia, Anthony Benjamin Borda Jr.

Theses, Dissertations and Capstones

Utilization of wood duck (Aix sponsa, L.) nest boxes was evaluated at the Green Bottom Wildlife Management Area, a 1,110-acre mid-Ohio Valley floodplain. Primary focus was directed upon nest box utilization by the wood duck but use by other wildlife was observed and recorded. Utilization of the nest boxes by the wood duck was analyzed with respect to plant community type, orientation and juxtaposition to other wood duck nest boxes. Forty-three wood duck next boxes were observed and inspected in 1993. Wood ducks nested in 24 of the nest boxes, and 361 eggs were found. Clutch size ranged …

Introduction: One Hundred Years Of Natural History In Turkmenistan, Victor Fet

Biological Sciences Faculty Research

As part of the famous “Great Game” between the Russian and British Empires in Central Asia, Turkmenistan was the last colonial prize of the Russian tsars; its delineation from Afghanistan was completed only in the 1890s. The Russian Empire’s Transcaspian Region (Zakaspiiskaya Oblast) was roughly what Turkmenistan is today; its neighbors were the semi-independent emirate of Bokhara to the east and khanate of Khiva to the north, both remnants of medieval Muslim empires.

This book combines the results of basic scientific research in biogeography and ecology; its purpose is to give a fairly comprehensive account of the nature of Turkmenistan. …

Aspects Of The Ecology Of The Wood Turtle, Clemmys Insculpta (Leconte), In West Virginia, Allan J. Niederberger

Theses, Dissertations and Capstones

An ecological study of the wood turtle, Clemmys insculpta (LeConte), was conducted in West Virginia from May 1991 to March 1993. Population structure at the primary study area (WV #1) was estimated to be 46% juvenile (H-8 yrs. old), 26% female and 28% male. Total population estimate for WV #1 was 337 wood turtles, with an aquatic density of 1,980 turtles per Hectare of river and a terrestrial density of 22.5 turtles per ha of terrestrial habitat. Growth measurements and estimates indicate rapid initial growth tapering off at year 8 and gradually declining through year 16. Males grow slightly faster …