Ecology and Evolutionary Biology Commons^™

Microsatellite Analysis Of Trophy Largemouth Bass From Arkansas Reservoirs, K. A. Lamothe, Ronald L. Johnson

Journal of the Arkansas Academy of Science

The Arkansas Game and Fish Commission (AGFC) has introduced Florida largemouth bass (FLMB; Micropterus salmoides floridanus) to water bodies historically containing the northern largemouth bass (NLMB; Micropterus salmoides salmoides) subspecies since the late 1970s in an attempt to produce a trophy LMB fishery. Since 2006, the AGFC has been biannually sampling reservoirs stocked with FLMB to determine levels of admixture. Here, total sampling efforts between 2006 and 2011 have been combined, and LMB heavier than 2,268 g (5 lb) were analyzed in an effort to investigate distribution of bass by their genetic composition designated as trophy LMB by the AGFC. …

Habitat Associations Of Stream-Dwelling Salamanders At Multiple Spatial Scales In Central Kentucky Headwaters., John Mark Yeiser

Online Theses and Dissertations

Headwater stream systems are productive habitats that are often at risk of human perturbation, and it is crucial that ecologists understand natural patterns and processes within these ecosystems. Using a multi-scale approach, I investigated factors influencing habitat associations of Ambystoma barbouri (streamside salamander) and Eurycea cirrigera (southern two-lined salamander) in a relatively undisturbed stream network in central Kentucky. I used likelihood ratio G-tests to identify associations between species and mesohabitat types (i.e., runs, riffles, and pools). I used second order Akaike's Information Criterion (AICc) model selection to predict patterns of presence and abundance throughout headwater reaches. Fine-scale abiotic …

Biodiversity, Biogeography, And Conservation Of Bats In The Lesser Antilles, Scott C. Pedersen, Hugh H. Genoways, Gary G. Kwiecinski, Peter A. Larsen, Roxanne J. Larsen

University of Nebraska State Museum: Mammalogy Papers

The chiropteran fauna of the Lesser Antilles consists of 27 species. The diversity of this fauna is low when compared with Neotropical faunas of large continental islands or at sites on the adjacent mainland, however, the Lesser Antillean fauna contains 11 species endemic to these islands making it worthy of large-scale conservation efforts. At the southern end of the Lesser Antilles, the biological limit of the Lesser Antillean bat fauna is marked by Koopman’s Line. To the south of this line, the bat faunas of the Grenadines and Grenada are composed of South American and widespread species of bats. The …

A Reassessment Of The Conservation Status Of A Critically Endangered Neotropical Frog, Mannophryne Olmonae, Using Occupancy Modeling Techniques, Jessica Mcquigg

Senior Independent Study Theses

Amphibian species worldwide are threatened with decline and extinction, making species monitoring an important scientific endeavor. The Bloody Bay Poison Frog, Mannophryne olmonae, a Tobago island endemic, was identified as critically endangered by the IUCN in 2004. Recent evidence suggests that a less severe conservation status may be appropriate for M. olmonae. This study employs acoustic calling surveys, land-use information, and multi-year (2011 and 2012) occupancy modeling techniques to propose an appropriate conservation status for this species. This study suggests that M. olmonae occupies a larger geographic range than was previously thought, and is not experiencing population declines. …

Influence Of Latitude On The Winter Abundance Of Red-Tailed Hawks (Buteo Jamaicensis) And American Kestrels (Falco Sparverius) In Illinois, Given Harper, Anna Groves, Vic Berardi, Paul Sweet, Jance Sweet, Angelo Capparella

Scholarship

We use five years of data from 18 routes surveyed to determine the temporal stability of a first reported 100 years pattern, and reconfirmed that the abundance of winter 50 years ago tailed Hawk and American Kestrel decreases with increasing latitude, being more high in the central regions of Illinos in the northern regions. Trained volunteers conducted sampling ( n  = 143) a month driving along selected routes from December to February, from 2004-2005 to 2008-2009. We found significant increases in the abundance of both species from the northern regions to the central regions of Illinois. No significant effects of …

A New Species Of Metathelazia (Nematoda: Pneumospiruridae) From The Lungs Of A Nine-Banded Armadillo In Central Mexico = Especie Nueva De Metathelazia (Nematoda: Pneumospiruridae) De Los Pulmones De Un Armadillo Del Centro De México, F. Agustín Jiménez-Ruiz, Rogelio Rosas-Valdez, Scott Lyell Gardner

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Twenty-four worms were collected from the bronchioles in both lungs of a male nine-banded armadillo Dasypus novemcinctus Linnaeus, 1758 captured in Teacalco, Morelos, Mexico. The worms, herein named Metathelazia mexicana n. sp., show a constriction in the esophagus at the level of the nerve ring; males of the species have 7 pairs of papillae, fewer than the other species in the genus. Metathelazia capsulata is the most similar species to Metathelazia mexicana; however, the latter has much shorter spicules. This is the fourth species in the genus known to occur in the New World.

Se recolectó un total de …

On The Morphology And Taxonomy Of Griphobilharzia Amoena Platt And Blair, 1991 (Schistosomatoidea), A Dioecious Digenetic Trematode Parasite Of The Freshwater Crocodile, Crocodylus Johnstoni, In Australia [Critical Comment], Thomas R. Platt, Eric P. Hoberg, Leslie A. Chisholm

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Griphobilharzia amoena Platt and Blair, 1991 was originally described was originally described as a dioecious trematode, parasitic in the circulatory system of the Australian freshwater crocodile, Crocodylus johnstoni, with the female completely enclosed in a gynecophoric chamber of the male and the two worms orientated anti-parallel to each other. A recent publication questions the original description, arguing that G. amoena is monoecious and, as a consequence, the species was transferred to Vasotrema Stunkard, 1928 (Spirorchiidae) as Vasotrema amoena n. comb. We provide photomicrographic evidence that the original description of G. amoena is correct and that Griphobilharzia Platt and Blair, …

The Effect Of Visual Wulst Lesions And Trigeminal Nerve Sectioning On The Discrimination Of Magnetic Inclination In The Homing Pigeon (Columba Livia), Merissa Acerbi

Honors Projects

The ability of homing pigeons to return to their loft from unknown places has fascinated scientists for centuries. It is well established that homing pigeons, like migratory birds, posses an innate magnetic inclination compass to determine direction by measuring the angle between the magnetic field vector and the Earth's surface. Recent work has indicated that the avian magnetic compass is light mediated and appears to mediate magnetic information to the brain. This occurs via a visual pathway with processing in the visual Wulst area of the forebrain. There is, however, also evidence from other avian species that magnetic direction may …

Mutation From Arginine To Lysine At The Position 189 Of Hemagglutinin Contributes To The Antigenic Drift In H3n2 Swine Influenza Viruses, Jianqiang Ye, Yifei Xu, Jillian Harris, Hailiang Sun, Andrew S. Bowman, Fred L. Cunningham, Carol Cardona, Kyoungjin J. Yoon, Richard D. Slemons, Xiu-Feng Wan

United States Department of Agriculture Wildlife Services: Staff Publications

Two distinct antigenic clusters were previously identified among the H3N2 swine influenza A viruses (IAVs) and were designated H3N2SIV-alpha and H3N2SIV-beta (Feng et al., 2013. Journal of Virology 87(13), 7655–7667). A consistent mutation was observed at the position 189 of hemagglutinin (R189K) between H3N2SIV-alpha and H3N2SIV-beta fair isolates. To evaluate the contribution of R189K mutation to the antigenic drift from H3N2SIV-alpha to H3N2SIV-beta, four reassortant viruses with189R or189K were generated. The antigenic cartography demonstrated that the R189K mutation in the hemagglutinin of H3N2IAV contributed to the antigenicdrift, separating these viruses into H3N2SIV-alpha to H3N2SIV- beta. This R189K mutation was also …

The Debate On Marine Mammals In Captivity, Lorna C. Scribner

Honors Theses

Are marine mammal species better off today because of captivity? Is captivity ethical and should it be continued? As this debate grows stronger, both of these sides of the argument offer substantial evidence in their favor. In this paper, I discuss data for both sides and evaluate the justifications of marine mammal captivity. Ideally, no matter the outcome, this research will educate the public on influential factors of wild and captive populations.

Five New Species Of Chaerilus Simon, 1877 From China, Indonesia, Malaysia, Philippines, Thailand, And Vietnam (Scorpiones: Chaerilidae), František Kovařík

Euscorpius

Chaerilus cimrmani sp. n. from Thailand, C. seiteri sp. n. from Philippines (Negros Island), C. solegladi sp. n. from Indonesia and Malaysia (Borneo Island), C. terueli sp. n. from Vietnam (Côn Son Island), and C. wrzecionkoi sp. n. from China (Tibet) are described. A key to all species of the genus Chaerilus Simon, 1877 is also presented.

Three New Species Of Compsobuthus Vachon, 1949 From Yemen, Jordan, Israel, And Somaliland (Scorpiones: Buthidae), František Kovařík

Euscorpius

Compsobuthus krali sp. n. of the werneri group from Yemen, C. levyi sp. n. of the werneri group from Jordan and Israel, and C. somalilandus sp. n. of the acutecarinatus group from Somaliland are described. A key to all species of the genus Compsobuthus Vachon, 1949 is presented.

Ghost Tiger Beetle (Cicindela Lepida): Species Conservation Assessment, Melissa J. Panella

Nebraska Game and Parks Commission: Publications

The primary goal in development of at-risk species conservation assessments is to compile biological and ecological information that may assist conservation practitioners in making decisions regarding the conservation of species of interest. The Nebraska Natural Legacy Project recognizes the ghost tiger beetle, a.k.a. white tiger beetle, (Cicindela lepida) as a Tier I at-risk species of high conservation priority. Some general management recommendations are made here regarding ghost tiger beetles; however, conservation practitioners will need to use professional judgment to make specific management decisions based on objectives, location, and a multitude of variables. This resource was designed to share available knowledge …

Molecular Phylogeny Of Microhylid Frogs (Anura: Microhylidae) With Emphasis On Relationships Among New World Genera, Rafael O. De Sá, Jeffrey W. Streicher, Relebohile Sekonyela, Mauricio C. Forlani, Simon P. Loader, Eli Greenbaum, Stephen Richards, Célio F.B. Haddad

Biology Faculty Publications

Background: Over the last ten years we have seen great efforts focused on revising amphibian systematics. Phylogenetic reconstructions derived from DNA sequence data have played a central role in these revisionary studies but have typically under-sampled the diverse frog family Microhylidae. Here, we present a detailed phylogenetic study focused on expanding previous hypotheses of relationships within this cosmopolitan family. Specifically, we placed an emphasis on assessing relationships among New World genera and those taxa with uncertain phylogenetic affinities (i.e., incertae sedis).

Results: One mitochondrial and three nuclear genes (about 2.8 kb) were sequenced to assess phylogenetic relationships. We utilized an …

Strike Mechanics Of An Ambush Predator: The Spearing Mantis Shrimp, M. Devries, E. Murphy, S. Patek

Faculty Publications, Biological Sciences

Ambush predation is characterized by an animal scanning the environment from a concealed position and then rapidly executing a surprise attack. Mantis shrimp (Stomatopoda) consist of both ambush predators (‘spearers’) and foragers (‘smashers’). Spearers hide in sandy burrows and capture evasive prey, whereas smashers search for prey away from their burrows and typically hammer hard-shelled, sedentary prey. Here, we examined the kinematics, morphology and field behavior of spearing mantis shrimp and compared them with previously studied smashers. Using two species with dramatically different adult sizes, we found that strikes produced by the diminutive species, Alachosquilla vicina, were faster (mean peak …

Buchholz Black Dash (Euphyes Conspicua Buchholzi): Species Conservation Assessment, Melissa J. Panella

Nebraska Game and Parks Commission: Publications

The primary goal in development of at-risk species conservation assessments is to compile biological and ecological information that may assist conservation practitioners in making decisions regarding the conservation of species of interest. The Nebraska Natural Legacy Project recognizes the Buchholz Black Dash (Euphyes conspicua buchholzi) as a Tier I at-risk species of high conservation priority. The Buchholz Black Dash (BBD) is a subspecies of the Black Dash (Euphyes conspicua). Survival of BBD is likely to be highly influenced by available habitat in Nebraska. Some general management recommendations are made here regarding the BBD; however, conservation practitioners will need to use …

Mccown’S Longspur (Rhynchophanes Mccownii): Species Conservation Assessment, Melissa J. Panella

Nebraska Game and Parks Commission: Publications

The primary goal in development of at-risk species conservation assessments is to compile biological and ecological information that may assist conservation practitioners in making decisions regarding the conservation of species of interest. The Nebraska Natural Legacy Project recognizes the McCown’s Longspur (Rhynchophanes mccownii) as a Tier I at-risk species. Some general management recommendations are made here regarding the McCown’s Longspur (MCLO); however, conservation practitioners will need to use professional judgment to make specific management decisions based on objectives, location, and a multitude of variables. This resource was designed to share available knowledge of MCLO that will aid in the decision-making …

Genetic Diversity Of Neotropical Myotis (Chiroptera: Vespertilionidae) With An Emphasis On South American Species, Roxanne J. Larsen, Michelle C. Knapp, Hugh H. Genoways, Faisal Ali Anwarali Khan, Peter A. Larsen, Don E. Wilson, Robert J. Baker

University of Nebraska State Museum: Mammalogy Papers

Background: Cryptic morphological variation in the Chiropteran genus Myotis limits the understanding of species boundaries and species richness within the genus. Several authors have suggested that it is likely there are unrecognized species-level lineages of Myotis in the Neotropics. This study provides an assessment of the diversity in New World Myotis by analyzing cytochrome-b gene variation from an expansive sample ranging throughout North, Central, and South America. We provide baseline genetic data for researchers investigating phylogeographic and phylogenetic patterns of Myotis in these regions, with an emphasis on South America.

Methodology and Principal Findings: Cytochrome-b sequences were generated and …

Spatial And Oceanographic Factors Affecting Black-Legged Kittiwake And Thick-Billed Murre Distributions In The Southeastern Bering Sea, Brian Hoover

Capstone Projects and Master's Theses

The distribution patterns of Thick-billed Murres (Uria lomvia) and Black-legged Kittiwakes (Rissa tridactyla) in the southeastern Bering Sea were documented at sea during vessel-based surveys in July-August of 2008 and 2009. The relationships of murre and kittiwake densities with environmental variables were investigated using binomial generalized additive models (GAMs) to model the presence or absence of birds, and Gamma-error distribution GAMs to model the positive densities of birds. Environmental variables included oceanographic factors (Chlorophyll, Chlorophyll anomalies, Daily SST, Monthly SST), spatial factors (Distance to nearest colony, Distance to 300m shelf break, Depth), and a temporal factor (Year). Nocturnal surveys were …

High Prevalence Of The Amphibian Chytrid Fungus (Batrachochytrium Dendrobatidis) Across Multiple Taxa And Localities In The Highlands Of Ethiopia, David J. Gower, Thomas M. Doherty-Bone, Roman K. Aberra, Abebe Mengistu, Silvia Schwaller, Michele Menegon, Rafael O. De Sá, Samy A. Saber, Andrew A. Cunningham, Simon P. Loader

Biology Faculty Publications

Surveys of the potentially lethal amphibian chytrid fungus (Batrachochytrium dendrobatidis - Bd) in Africa are patchy, especially in some regions of high species endemicity. We present results of the first Bd surveys of wild amphibians in Ethiopia, for two upland regions on either side of the Rift Valley: the Bale Mountains and the Kaffa region. Surveys were opportunistic so that robust interpretation of the data is limited. Utilizing diagnostic qPCR assays, 51 out of 120 frogs (14 species in 10 genera) tested positive for Bd at altitudes of 1,620–3,225 m, across all genera and species, and all but …

Euscorpius Sicanus (Scorpiones: Euscorpiidae) From Tunisia: Dna Barcoding Confirms Ancient Disjunctions Across The Mediterranean Sea, Matthew R. Graham, Pavel Stoev, Nesrine Akkari, Gergin Blagoev, Victor Fet

Biological Sciences Faculty Research

We used a DNA barcoding marker (mitochondrial cox1) to investigate the controversial natural occurrence of Euscorpius sicanus (C.L. Koch) in North Africa. We tested this hypothesis by comparing a sample collected from a mountain in Tunisia to disjunct populations in Sardinia, Malta, and Greece. Using these samples, and a few additional Euscorpius spp. from southern Europe as outgroups, we reconstructed the maternal phylogeny. We then used a molecular clock to place the phylogeny in a temporal context. The Tunisian sample grouped closest to a specimen from Sardinia, with both being more distantly related to E. sicanus from Malta, which is …

Plains Harvest Mouse (Reithrodontomys Montanus Griseus): Species Conservation Assessment, Melissa J. Panella

Nebraska Game and Parks Commission: Publications

The primary goal in development of at-risk species conservation assessments is to compile biological and ecological information that may assist conservation practitioners in making decisions regarding species of interest. The Nebraska Natural Legacy Project recognizes the plains harvest mouse (Reithrodontomys montanus griseus) as a Tier I at-risk species of high conservation need. Some general management recommendations are made here regarding the plains harvest mouse; however, conservation practitioners will need to use professional judgment to make specific management decisions based on objectives, location, and a multitude of variables. This resource was designed to share available knowledge of the plains harvest mouse …

Topeka Shiner (Notropis Topeka): Species Conservation Assessment, Melissa J. Panella

Nebraska Game and Parks Commission: Publications

The primary goal in development of at-risk species conservation assessments is to compile biological and ecological information that may assist conservation practitioners in making decisions regarding the conservation of species of interest. The Nebraska Natural Legacy Project recognizes the Topeka shiner (Notropis topeka) as a Tier I at-risk species of high priority for conservation. Some general management recommendations are made here regarding Topeka shiners; however, conservation practitioners will need to use professional judgment to make specific management decisions based on objectives, location, and a multitude of variables. This resource was designed to share available knowledge of the Topeka shiner that …

The Development Of Dermatonotus Muelleri (Anura: Microhylidae: Gastrophryninae), Marissa Fabrezi, Silvia Quinzio, Javier Goldberg, Rafael O. De Sá

Biology Faculty Publications

The monophyly of Microhylidae is supported by an overwhelming accumulation of synapomorphic larval features. Despite the distinctiveness of the microhylid tadpole, few studies have focused on larval development. Microhylid larval morphology is usually described and based on standard tables that imply that developmental events at equivalent stages of overall tadpole development are independent from species-specific patterns of developmental timing. Herein, we present additional developmental data based on external morphology and field data on larval growth for the gastrophrynine microhylid Dermatonotus muelleri. We describe internal morphological variation (e.g., skeletal and soft systems) during larval development. The results indicate that the onset …

Climate's Role In Polar Bear Past [Letters], Kurt E. Galbreath, Joseph A. Cook, Eric P. Hoberg

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Striking temporal concordance between the new date for divergence of polar bears and persistent freezing of the Arctic Ocean suggests that this may be one of relatively few instances in which a specific paleoclimatological episode can be convincingly linked to a specific evolutionary event, and it provides vivid demonstration of climatic forcing as a determinant of diversification in biological systems.

Stable Isotope Analysis (Delta Nitrogen-15 And Delta Carbon-13) And Bioenergetic Modeling Of Spatial-Temporal Foraging Patterns And Consumption Dynamics In Brown And Rainbow Trout Populations Within Catch-And-Release Areas Of Arkansas Tailwaters, Jon M. Flinders

Graduate Theses and Dissertations

I examined spatial and temporal consumption dynamics using an energy intake model and a bioenergetics model of rainbow trout, Oncorhynchus mykiss, and brown trout, Salmo trutta, within three catch-and-release (C-R) areas in Bull Shoals and Norfork tailwaters to determine whether trout populations were limited by food supply. I also examined the seasonal and ontogenetic shifts in the foraging patterns of brown and rainbow trout within these areas using gut content analysis (GCA) and stable isotope analysis (SIA) of C and N. I examined 605 brown trout and 768 rainbow trout for GCA and SIA at Bull Shoals, Norfork, …

The Origin Of Malarial Parasites In Orangutans, M. Andreína Pacheco, Michael J.C. Reid, Michael A. Schillaci, Carl A. Lowenberger, Biruté M. F. Galdikas, Lisa Jones-Engel, Ananias A. Escalante

Harold W. Manter Laboratory of Parasitology: Library Materials

Background: Recent findings of Plasmodium in African apes have changed our perspectives on the evolution of malarial parasites in hominids. However, phylogenetic analyses of primate malarias are still missing information from Southeast Asian apes. In this study, we report molecular data for a malaria parasite lineage found in orangutans.

Methodology/Principal Findings: We screened twenty-four blood samples from Pongo pygmaeus (Kalimantan, Indonesia) for Plasmodium parasites by PCR. For all the malaria positive orangutan samples, parasite mitochondrial genomes (mtDNA) and two antigens: merozoite surface protein 1 42 kDa (MSP-1₄₂) and circumsporozoite protein gene (CSP) were amplified, cloned, and …

Observations Of Oviposition Behavior Among North American Tiger Beetle (Coleoptera: Carabidae: Cicindelinae) Species And Notes On Mass Rearing, Mathew L. Brust, C. Barry Knisley, Stephen M. Spomer, Kentaro Miwa

University of Nebraska State Museum: Entomology Papers

Although the larvae of a large number of North American tiger beetle species have been described, little information exists on the oviposition behavior of female tiger beetles at the species level. In this study, we found that the oviposition behavior varied widely among species, with some species utilizing more than one method. We found that, contrary to many references, several tiger beetle species did not oviposit from above the soil surface by inserting the ovipositor into the soil. Instead, several species oviposited by tunneling over 20 mm below the soil surface. In addition, we outline recently used successful protocols for …

Notes On The Diet Of The Malay Civet (Viverra Tangalunga) And Other Civets In Logged And Unlogged Lowland Dipterocarp Rain Forests In Sabah, Borneo, Christina P. Colon, John B. Sugau

Publications and Research

Civet diets were examined in a logged and unlogged Bornean rain forest. Malay civets (Viverra tangalunga) consumed invertebrates, fruit, rodents, insectivores, birds, snakes and lizards, and appear to show preference for centipedes and scorpions. Other civet species consumed fruit, such as figs, Connarus sp. and Annona sp., particularly in the unlogged forest, but also consumed invertebrates and vertebrates. Reduced fruit consumption observed in the logged forest may be due to lower availability and may be offset by increased consumption of invertebrates. The increased overlap in diet between Malay civets and other civets in disturbed areas may lead to …

A High Density Snp Array For The Domestic Horse And Extant Perissodactyla: Utility For Association Mapping, Genetic Diversity, And Phylogeny Studies, Molly E. Mccue, Danika L. Bannasch, Jessica Lynn Petersen, Jessica Gurr, Ernie Bailey, Matthew M. Binns, Ottmar Distl, Gérard Guérin, Telhisa Hasegawa, Emmeline W. Hill, Tosso Leeb, Gabriella Lindgren, M. Cecilia T. Penedo, Knut H. Røed, Oliver A. Ryder, June E. Swinburne, Teruaki Tozaki, Stephanie J. Valberg, Mark Vaudin, Kerstin Lindblad-Toh, Claire M. Wade, James R. Mickelson

Department of Animal Science: Faculty Publications

An equine SNP genotyping array was developed and evaluated on a panel of samples representing 14 domestic horse breeds and 18 evolutionarily related species. More than 54,000 polymorphic SNPs provided an average inter-SNP spacing of ~43 kb. The mean minor allele frequency across domestic horse breeds was 0.23, and the number of polymorphic SNPs within breeds ranged from 43,287 to 52,085. Genome-wide linkage disequilibrium (LD) in most breeds declined rapidly over the first 50–100 kb and reached background levels within 1–2 Mb. The extent of LD and the level of inbreeding were highest in the Thoroughbred and lowest in the …