Open Access. Powered by Scholars. Published by Universities.®
- Discipline
- Keyword
Articles 1 - 3 of 3
Full-Text Articles in Cancer Biology
Mcnamara 201412 Nih Scap Innocentive Challenge Solution - T-Bow Rainbow T-Cells And Tumor Cells Spatial Multiplexing Gene Expression Reporter System – Plus Supplement Plus Posters - 20151027 - Please Download "75" Instead, George Mcnamara
George McNamara
McNamara 201412 NIH SCAP InnoCentive Challenge Solution - T-Bow Rainbow T-cells and Tumor Cells Spatial Multiplexing Gene Expression Reporter System – plus supplement plus posters - 20151027.
///
Please download the current 20151027 (October 27, 2015) Tattletales and T-Bow update from
http://works.bepress.com/gmcnamara/75/
The bepress web site is not letting me replace the old pdf here at "65" with the additional 10 pages update.
///
The download is my/Cooper lab solution (submission) to the 2014 NIH Single Cell Analysis Program (SCAP) InnoCentive Challenge, "Follow That Cell". I submitted the Solution on 20141215Mon (with 20 minutes to spare). The Challenge web page …
Tattletales And T-Bow Update 20151027tue, George Mcnamara
Tattletales And T-Bow Update 20151027tue, George Mcnamara
George McNamara
20151027Tue this "75"
http://works.bepress.com/gmcnamara/75
is my update of "65" posting
See text at
http://works.bepress.com/gmcnamara/65/
for text summary. The PDf here in "75" supersedes "65".
The PDF here has 10 pages added to the end from the "65" version (pages 40-49 of PDF when including the bepress cover page)..
here is the text in my cover page (bepress may add its own cover):
20151027Tue: added 10 page e-poster at bottom explaining Binary Tattletales and T-Bow. That is, binary with respect to protein components. For one color (number of repeats, epitope tags, FPs are examples, here rounded to convenient numbers):
1. 100 …
Light Microscopy Reflection Focusing Cells And Coverglass, George Mcnamara
Light Microscopy Reflection Focusing Cells And Coverglass, George Mcnamara
George McNamara
Light Microscopy Reflection Focusing Cells and Coverglass 20150520Wed
Many light microscopes have the capability of using camera based brightfield, phase contrast, or fluorescence to find the most contrasty specimen focus. When I worked for UIC (1992-1997) we offered this with a combination of Ludl MAC2000 controller, Z-drive, video card thingy, and video camera (most customers at the time used analog video cameras).
Many automated light microscopes now have clever -- and pricey -- autofocus systems based on an NIR LED structured illumination reflecting light off the bottom of the coverglass.
I encourage a simpler approach - my hardware details are …