Open Access. Powered by Scholars. Published by Universities.®
Cell and Developmental Biology Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Discipline
- Publication
- Publication Type
Articles 1 - 4 of 4
Full-Text Articles in Cell and Developmental Biology
Differential Expression Of Mtor Related Molecules In The Placenta Of Patients With Gestational Diabetes Mellitus, Intrauterine Growth Restriction, Or Preeclampsia, Katherine Price, Brent Kimbler, Nekel Knowlton, Kelsey Hirschi, Paul Reynolds, Juan Arroyo
Differential Expression Of Mtor Related Molecules In The Placenta Of Patients With Gestational Diabetes Mellitus, Intrauterine Growth Restriction, Or Preeclampsia, Katherine Price, Brent Kimbler, Nekel Knowlton, Kelsey Hirschi, Paul Reynolds, Juan Arroyo
Student Works
The mechanistic target of rapamycin (mTOR) pathway is involved in placental growth and function during pregnancy. The mTOR pathway responds to nutrient availability and growth factors regulating protein transcription and cell growth. mTOR disruptions are associated with the development of obstetric complications which may result in adverse health outcomes for the mother and/or fetus. The purpose of this study is to identify the differing placental expression of various mTOR-associated proteins during normal gestation (Control), gestational diabetes mellitus (GDM), intrauterine growth restriction (IUGR) and preeclampsia (PE). Immunohistochemistry was used to stain human placenta for activated proteins (phospho; p)AKT, (p)ERK, (p)mTOR, (p)p70 …
Regulation Of Trophoblast Invasion By Pyruvate Kinase Isozyme M2 (Pkm2), Juan Mejia, Parker Hall, Kelsey Hirschi, Paul R. Reynolds, Juan Arroyo
Regulation Of Trophoblast Invasion By Pyruvate Kinase Isozyme M2 (Pkm2), Juan Mejia, Parker Hall, Kelsey Hirschi, Paul R. Reynolds, Juan Arroyo
Student Works
The Pyruvate kinase isozymes M2 (PKM2) protein is a metabolic enzyme that regulates the final step of glycolysis. This enzyme is present in highly proliferating cells such as those present in embryonic tissues, cancer and in the placental trophoblast. We recently studied PKM2 protein in the human placenta at term. Our results demonstrated upregulated placental PKM2 during preeclampsia (PE) where trophoblast invasion is decreased and hypoxia is present. Our objective was to determine the cytosolic and nuclear PKM2 expression pattern and determine PKM2 regulation of trophoblast cell invasion. Trophoblast cells were cultured in normoxic and hypoxic conditions and real time …
The Role Of Hypoxia On Pyruvate Kinase M2, Mammalian Target Of Rapamycin, Mitochondrial Function, And Cell Invasion In The Trophoblast, Rebecca Lutz Kimball
The Role Of Hypoxia On Pyruvate Kinase M2, Mammalian Target Of Rapamycin, Mitochondrial Function, And Cell Invasion In The Trophoblast, Rebecca Lutz Kimball
Theses and Dissertations
This thesis will be organized into two chapters discussing the role of hypoxia in the human placenta. The goal of this thesis is to characterize pyruvate kinase M2, mammalian target of rapamycin, mitochondrial function, and cell invasion in hypoxic conditions in the trophoblast. Understanding the mechanisms of placental metabolism can lead to further treatments for placental diseases. Chapter one covers the background of intrauterine growth restriction, hypoxia, placental metabolism, and pyruvate kinase M2 (PKM2). Little is currently understood about the role of the mitochondria in placental diseases. Expression of PKM2, trophoblast cell invasion, and mitochondrial function is shown to be …
Different Expression Of Placental Pyruvate Kinase M2 In Normal, Preeclamptic, And Intrauterine Growth Restriction Pregnancies, Brigham L. Bahr
Different Expression Of Placental Pyruvate Kinase M2 In Normal, Preeclamptic, And Intrauterine Growth Restriction Pregnancies, Brigham L. Bahr
Theses and Dissertations
This thesis will be organized into two chapters discussing the placental expression of two proteins, pyruvate kinase M2 (PKM2) and heat shock protein 27 (HSP 27), in human placentas. Understanding the mechanisms of placental metabolism in healthy and diseased placentas helps us understand how placenta disorders occur and how we can treat these disorders. The goal is to investigate these proteins to gain an understanding of their roles in placental disorders and help decrease maternal and fetal mortality rates. Chapter one covers the background of pyruvate kinase M2 (PKM2) in cancer and embryonic tissues, and the expression of PKM2 in …