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Full-Text Articles in Cell and Developmental Biology
Differential Expression Of Mtor Related Molecules In The Placenta Of Patients With Gestational Diabetes Mellitus, Intrauterine Growth Restriction, Or Preeclampsia, Katherine Price, Brent Kimbler, Nekel Knowlton, Kelsey Hirschi, Paul Reynolds, Juan Arroyo
Differential Expression Of Mtor Related Molecules In The Placenta Of Patients With Gestational Diabetes Mellitus, Intrauterine Growth Restriction, Or Preeclampsia, Katherine Price, Brent Kimbler, Nekel Knowlton, Kelsey Hirschi, Paul Reynolds, Juan Arroyo
Student Works
The mechanistic target of rapamycin (mTOR) pathway is involved in placental growth and function during pregnancy. The mTOR pathway responds to nutrient availability and growth factors regulating protein transcription and cell growth. mTOR disruptions are associated with the development of obstetric complications which may result in adverse health outcomes for the mother and/or fetus. The purpose of this study is to identify the differing placental expression of various mTOR-associated proteins during normal gestation (Control), gestational diabetes mellitus (GDM), intrauterine growth restriction (IUGR) and preeclampsia (PE). Immunohistochemistry was used to stain human placenta for activated proteins (phospho; p)AKT, (p)ERK, (p)mTOR, (p)p70 …
Regulation Of Trophoblast Invasion By Pyruvate Kinase Isozyme M2 (Pkm2), Juan Mejia, Parker Hall, Kelsey Hirschi, Paul R. Reynolds, Juan Arroyo
Regulation Of Trophoblast Invasion By Pyruvate Kinase Isozyme M2 (Pkm2), Juan Mejia, Parker Hall, Kelsey Hirschi, Paul R. Reynolds, Juan Arroyo
Student Works
The Pyruvate kinase isozymes M2 (PKM2) protein is a metabolic enzyme that regulates the final step of glycolysis. This enzyme is present in highly proliferating cells such as those present in embryonic tissues, cancer and in the placental trophoblast. We recently studied PKM2 protein in the human placenta at term. Our results demonstrated upregulated placental PKM2 during preeclampsia (PE) where trophoblast invasion is decreased and hypoxia is present. Our objective was to determine the cytosolic and nuclear PKM2 expression pattern and determine PKM2 regulation of trophoblast cell invasion. Trophoblast cells were cultured in normoxic and hypoxic conditions and real time …