Cell and Developmental Biology Commons^™

Buoyancy Changes As Related To Respiratory Behavior In An Amphibious Snail, Pomacea Urceus (Müller), From Venezuela, Kathleen A. Burky, Albert J. Burky

Biology Faculty Publications

The ampullariid Pomacea urceus uses its ctenidium and lung in respiration. The snails reach the surface with their siphon and ventilate their lung by withdrawing their head-foot in a pumping action. The lung gas also serves to change the buoyancy of the snails. After a ventilation a snail does not necessarily return to the same buoyancy level. They may achieve overall specific gravities of <1 to > 1 after a ventilation. They may remain submerged (s.g. > 1) or float (s.g.

The Effect Of Cv-1 Cell Geometry On The Ultraviolet Radiation Sensitivity Of Mammalian Cells In Vitro, Daniel J. Knauer

Masters Theses & Specialist Projects

The effect of cell geometry on the radiosensitivity to ultraviolet (UV) light has been studied in the CV-1 host cell-capacity system. In this system the macromolecular damage incurred by monolayers of cells cultures as the result of exposure to UV radiation is monitored by testing the ability of irradiated cells to support the replication of Herpes simplex virus. The target molecule has been shown to be DNA and is centrally located

Mammalian cells have an absolute requirement for Mg⁺⁺ and Ca⁺⁺ in order to remain attached to artificial substrata. To test the effect of cell geometry on UV …

A Cytogenetic Study Of Xx/Xy Chimerism And Other Anomalies Of Bos Taurus In A Sampled Dairy Herd, Ferne M. Rogers

Masters Theses

The establishment of normal chromosomal patterns for a wide range of plants and animals has led to the recognition of abnormalities as well as their relationship to phenotypic irregularities. Research dealing with the expression of human karyotype is of prime interest; but, closely rivaling that is the genetic study of domestic animals which are of economic importance to man.

One observed anomaly which has come under scrutiny by cytogeneticists is that of the female member of a heterogeneous bovine twin pair, the freemartin. This is a sterile animal with internal morphology showing varying degrees of intersex. Cytogenetic studies reveal XX/XY …

Localization Of Neurosecretory Cells Within The Cerebral Ganglia Of Amphiphorus Imparispinosus Griffin, 1898 (Hoplonemertea) And Their Possible Regulatory Role In The Annual Reproductive Cycle, Gail H. Jacobson

Dissertations and Theses

Nemerteans are common invertebrates along coastlines in both temperate and arctic climates throughout the world. Amphiphorus imparispinosus is a carnivorous member of this phylum that is found abundantly in the intertidal areas of the Oregon coast. As in many other invertebrate phyla, endocrine integration within the nemerteans is thought to be accomplished by neurosecretory mechanisms. In Amphiphorus the presence of these neurosecretory systems has not been previously shown. This project was undertaken to determine if neurosecretory cells are present within the central nervous system of this species and. further, to examine seasonal changes in the number and appearance of these …

The Dispersal And Dispersion Patterns Of Hydra Fusca In A Limited Environment, Faith E. Ruffing

Dissertations and Theses

The dispersal and dispersion patterns of Hydra fusca were examined. Hydra were placed in petri dishes at various densities. The water in the dishes was swirled forcing the animals to the center. The location of each animal was marked at time intervals thereafter. Analyses of the dispersal rates and dispersion patterns were made. Hydra dispersed from a central release point at a non-random rate. There was rapid movement from the center followed by a minimal daily movement. This eventually resulted in a uniform dispersion pattern at high densities in a limited environment. There was a relationship between the ratio of …

Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoretic Studies Demonstrating Cytoplasmic Protein Components Unique To The Mycelial Phase And To The Yeast Phase Of Candida Albicans, Theresa Catherine Burgess

Biological Sciences Theses & Dissertations

Blastospore and mycelial cytoplasmic extracts from four strains of Candida albicans, varying in virulence, were compared by sodium dodecyl sulfate (SDS) polyacrylamide disc gel electrophoresis Cytoplasmic extracts were made by disrupting the cultures in a Braun homogenizer followed by ultracentrifugation. The supernatant fractions were then resolved on polyacrylamide gels containing SDS. Each of the preparations yielded a distinct pattern. There were mycelial specific and blastospore specific protein bands. There were more components resolved from the blastospore phase cytoplasmic extracts than from the mycelial phase cytoplasmic extracts. There were bands common to both phases. These results demonstrate protein components unique …

Scanning Electron Microscope Study Of Brachysclereids Of Pear (Pyrus Communis L.), Clarence B. Sinclair

Journal of the Arkansas Academy of Science

The external surfaces of pear sclereids commonly are illustrated as covered with apertures. This SEAA investigation of the surface features has shown the surface to have few or no apertures. When the primary wall layer was removed the typical ramiform canal system was obvious. This observation confirms the often-ignored fact that the pitapertures of the secondary wall are not continuous with the primary wall. Hence, they do not show on the surfaces of the intact cell.

The Effect Of Caffeine On Pisum Sativum With Emphasis On Meiosis, Terese M. Mikottis

Masters Theses

No abstract provided.

A Fine-Structural Study Of Aberrant Meiotic Cytokinesis In An Autosomal Male Sterile Mutant (Ms(2)3r) Of Drosophila Melonogaster, Laura J. Laughran

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The ultrastructure of abnormal meiotic cytokinesis in an autosomal male sterile mutant of Drosophila melanogaster is described. In the mutant ms(2)3R mitotic cytokinesis proceeds normally, but abnormal meiotic divisions give rise to four spermatids developing in one cytoplasmic mass. The contractile ring which is responsible for cell constriction during cytokinesis appears to form and function normally during meiosis. Ring canals which form intercellular bridges between synchronously developing spermatids are also apparently normal. However, there is an apparent adhesion and subsequent disintegration of the furrow membranes after division is completed. In addition to double membrane fragments in areas of open …

Light And Electron Microscope Study Of The Mitotic Apparatus Of The Ring-Legged Earwig, Euborellia Annulipes (Lucas), Vonnie R. Prentice, William L. Evans

Journal of the Arkansas Academy of Science

The mitotic apparatus in dividing cells of the ring-legged earwig is composed of the cell center or pole, chromosomal fibers, continuous fibers, and chromosomal and background structure. Parts from cellular elements such as Golgi membranes, endoplasmic reticulum, and nuclear envelope as well as whole mitochondria associate with the spindle microtubules to produce the diffraction pattern of the spindle fibers as seen with the light microscope. The microtubules of the chromosomal fibers attach independently and demonstrate the diffuse nature of the centromere or polycentric condition of the chromosomes.

Comparison Of Interferon Produced In Vitro By Monkey Kidney Cells Demonstrating Interference Against Polio Ii, Coxsackie A-9, Influenza A2/Hong Kong/68, And Echo-9 Viruses, Archer Duncan Farmer Jr.

Biological Sciences Theses & Dissertations

Abstract unavailable

Cytotypes And Morphometrics Of Two Phyllostomatid Bats, Micronycteris Hirsuta And Vampyressa Pusilla, Robert J. Baker, Hugh H. Genoways, William J. Bleier, Jerry W. Warner

University of Nebraska State Museum: Mammalogy Papers

Jackson and Patton stated that the key to understanding mechanisms of chromosomal evolution lies in studies of chromosomal variation within populations that can hybridize. Hybrids between cytotypes contain the answer to problems of meiotic pairing and reduced fertility, as well as information concerning the nature of zones of contact. However, before detailed studies can be initiated, chromosomal races and hybrid zones must be located. Few chromosomal races have been described for bats. Within the family Vespertilionidae, five species are known to have such races and races have been reported in two species of the family Phyllostomatidae.

Described are chromosomal races …

Cytogenic Studies Of A Chesapeake Bay Population Of Molgula Manhattensis (Dekay) (Ascidiacea: Stolidobranchia), Elizabeth A. Crawford

Biological Sciences Theses & Dissertations

Abstract unavailable.

Modification Of Ontogenic Expression Due To Relocation Of The Ry+ Gene In Drosophila Melanogaster, Allen A. Badgett Jr.

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The ry⁺ gene of Drosophila melanogaster and its associated enzyme, xanthine dehydrogenase, were employed in an analysis of the relationship between regulation and the location of a gene in the genome. Enzyme assays as an indication of gene activity were performed on genotypes containing zero, one and two doses of ry⁺ genes. Xanthine dehydrogenase activity of rosy genes in normal and relocated positions was determined during development.

The results indicate: 1) the gene ry⁺ is differentially active during ontogeny; 2) the activity during ontogeny is proportional to the number of ry⁺ genes present in the genome; …

Isolation And Characterization Of Two Bacteriophages Infecting The Myxobacterium Cytophaga Succinicans, Kenneth Wayne Lee

All Master's Theses

Two bacteriophages which infect the myxobacterium Cytophaga succinicans, strains 14 and 16, were isolated from fish hatchery effluent. Each was submitted to a detailed study. These phages have a limited host range, even within the genus Cytophaga. The turbid plaques formed by these phages are similar in appearance to those produced by coliphage Lambda, and average 0.85 mm in diameter. Both phages have a latent period of 195 minutes and a rise period of an additional 165 minutes. Phage 0CS-16 has a burst size of 137 and phage 0CS-14 has a burst of 45. Sodium ion at a concentration of …

Induction And Initial Characterization Of Male-Sterile Mutants On The Second Chromosome Of Drosophila Melanogaster, Susan M. Graves

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Fourteen recessive, male-sterile, autosomal mutants of Drosophila melanogaster were induced using ethyl methanesulfonate. The techniques employed restricted the recovery of mutants to those located on the second chromosome. Eleven of the mutants were male-sterile at 23°C, and the remaining three, though fertile at 23°C, were male-sterile at 28°C. Complementation tests involving all possible pairs of mutations showed each to be located in a unique cistron.

Spermiogenesis in each mutant was studied by light microscopy to determine the stage at which cellular differentiation deviates from normal and to characterize the associated gross abnormalities. Phase contrast microscopy showed that each mutant sterile …

Spermiogenesis In Two Male-Sterile Third-Chromosome Mutants Of Drosophila Melanogaster, Richard F. Wilkinson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

An ultrastructural study of sperm differentiation was made on two male-sterile mutants of Drosophila melanogaster. The mutations were induced on the third chromosome with the monoalkylating agent ethyl methanesulfonate and were maintained in stock cultures over a balancer chromosome using a system of multiple inversions. The stages of sperm differentiation, a most dramatic form of cellular differentiation, were then studied at the electron microscope level to determine the effect of the mutations on sperm structure and to correlate those changes with normal sperm development.

Studies of the genetic control of cellular differentiation have been hampered by difficulties in devising …

The Effects Of The Cyclic 3'5'-Monophosphate Of Adenosine And Its Dibutyryl Derivative On The In-Vitro Immunization Of Mouse Spleen Cell Suspensions, James A. Blanks

Biological Sciences Theses & Dissertations

Abstract unavailable.

A Comparative Consideration Of Lipoprotein Distribution And Stress Response In The Mallard Duck And Man, Janice Mackay

Honors Theses

Lipid is associated with all of the major serum protein fractions of both the mallard duck and man. however, whereas the ρ lipoproteins predominate in man with little lipo-albumin evident, according to available literature, in ducks the lipo-albumin constitutes more than half of the serum lipoproteins, and a substantial lipo-prealbumin fraction is evident. Most studies of lipo-proteins are carried out on human serum primarily for the purpose of diagnosis of abnormalities. The stress response in many animals includes a rise in serum α-globulin levels, but its physiological basis is still speculative. While a consideration of the changes in lipoprotein distribution …

The Role Of D-Gluconic Acid In The Regulation Of The Synthesis Of The Enzymes Of The Entner-Doudoroff Pathway In Pseudomonas Fluorescens, Steven C. Quay

Honors Theses

Induction of Entner-Duodoroff pathway enzymes in Pseudomonas fluorescens was investigated to study the role of gluconate as a possible inducer. Glucose oxidase-deficient mutants were isolated and characterized. One of these mutants, gox-7, was deficient in particulate glucose oxidase; another mutant, gox-17, was deficient in particulate glucose and gluconate oxidase activities. Gluconate, but not glucose, induced synthesis of gluconokinase and 6-phosphogluconate dehydratase in both mutants. High constitute levels of 2-keto-3-deoxy-6-phosphogluconate aldolase were found when both mutants were grown on glucose. Growth of parent and both mutant strains on glycerol also resulted in high levels of Entner-Doudoroff pathway enzymes. It was concluded …

Rapid Electroosmosis Measurements, James O. Wear

Journal of the Arkansas Academy of Science

A cell has been designed and built that allows for rapid measurement of volume moved in a definite time by electroosmosis. The cell is simple to use and is not very elaborate. Using a water jacket, the cell temperature can be controlled to ± 0.1° C. Measurements are presented for acetonitrile, dimethylformamide, and nitrobenzene at 25° C for applied voltages of 25, 50, 75, and 100 volts.

Effects Of Urethan On Fish Epithelial And Fibroblast Cells In Vitro, Leland F. Morgans

Journal of the Arkansas Academy of Science

The effects of urethan on RTG-2 and FHM cells were studied in vitro, by using the mitotic index, it was determined that 0.3 percent urethan caused an increase in the rate of cell division while higher concentrations (0.6, 0.9, 1.2, and 1.5 percent) caused either a decrease in the rate or a cessation of cell division. Concentrations of urethan higher than 1.5 percent killed the cells. The mitotic index data also indicated that epithelial cells continued to divide at a higher concentration of urethan than did the fibroblast cells. The morphological effects of urethan on the two cell lines were …

Permeability Of Broken-Cell Foamed Materials, J. P. Bosscher, F. E. Fisher

University Faculty Publications and Creative Works

No abstract provided.

Hemolysis By Crotalus Horridus Atricaudatus Venom, Franklin E. Byrd, Bob D. Johnson

Journal of the Arkansas Academy of Science

No abstract provided.

Microirradiation Of Living Fungal Cells With A Laser, John L. Robertson, Charles L. Wilson

Journal of the Arkansas Academy of Science

No abstract provided.

Effects Of Hydroxyurea On Cultured Somatic Cells Of The Chinese Hamster, Cricetulus Griseus, Charlotte Neill, William C. Guest

Journal of the Arkansas Academy of Science

No abstract provided.

Proposed Mechanisms Of Asexual Nuclear Division In Neurospora Crassa Shear And Dodge, Charles L. Wilson, John A. Brushaber, James R. Aist

Journal of the Arkansas Academy of Science

No abstract provided.

Observations On Nuclear Division In Vegetative Hyphae Of Ceratocystis Fagacearum, James R. Aist, Charles L. Wilson

Journal of the Arkansas Academy of Science

No abstract provided.

Synthetic Investigations In Steroids, Harold R. Gollberg

Theses

The term hormone may be define as any substance normally produced and secreted by cells in one part of the body and carried by the blood stream to some other part where it is effective in low concentrations in regulating and co-ordinating the activities of the cells. However, hormones cannot be defined as belonging to any particular class of chemicals. All hormones are organic substances, but some are proteins while others are relatively simpler chemical compounds, such as amino acids and steroids. The hormones secreted by the pituitary gland are proteins, while those of the thyroid are amino acids. Others, …

Some Effects Of Ionizing Radiation On The Oral Suckers Of Rana Pipiens Embryos, Charles C. Reed

Journal of the Arkansas Academy of Science

No abstract provided.