Cell and Developmental Biology Commons^™

Phasic Response Of Peripheral Nerves To Inflammation, Ralph Lee Myers

University of the Pacific Theses and Dissertations

Acute inflammation is associated with injury to tissues, whether it be from mechanical destruction, or from the introduction of a noxious substance into the tissue. Inflammation is a homeostatic process, adapted to neutralize harmful substances and restore injured tissue. Celsus (c. A.D. 178) provided the first documented characterization of inflammation. He recognized redness, heat, swelling and pain as the cardinal signs of the inflammatory reaction. Loss of function was later added to this list of symptons (Movat. 1971).

The Effect Of Parental Bone Marrow & Spleen Cells In The Induction Of The Graft-Versus-Host Reaction, Ronald Grane

Masters Theses & Specialist Projects

Variously aged (C57BL X C3H)F₁ hybrid mice were used as recipients in experiments to determine if spleen or bone marrow cells from normal C57BL or C3H donors, spleen or bone marrow cells from C57BL donors previously treated with cortisone, or C57BL bone marrow cells previously incubated with serum from X-irradiated C57BL mice could induce a graft-versus-host reaction (GVHR).

The mice were assayed for GVHR by mortality or splenomegaly as detected by the discriminant spleen assay developed by Simonsen. Results show that spleen cells from normal C57BL, normal C3H, or cortisone-treated C57BL mice are capable of inducing a GVHR in …

The Use Of Rat Liver Cell Cultures For The Isolation Of Histoplasma Capsulatum Darling, Allen Robert Riebau

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science by Allen Robert Riebau on May 12, 1978.

Requirement For Protein Synthesis In Adrenocorticotropin Stimulated Steroidogenesis Of Isolated Rat Adrenal Cells, John G. Menke

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by John G. Menke on May 6, 1978.

The Biology Of Coprinus Radians, Ozonium, Oidium, And Their Interrelationships, Thomas J. Fahey

All-Inclusive List of Electronic Theses and Dissertations

The pleomorphism of Coprinus radians was studied. The three distinct growth forms--sporophore, oidial stage, and ozonium stage--were examined in relation to ultrastructure, morphology and development. Scanning and transmission electron microscopy are correlated with photomicrographs of developmental stages. Development of the oidia and of the sporophore are of particular interest. The multinucleate oidia develop quite differently from oidia as generally described for Basidiomycete imperfect stages. Development of the oidia of this Coprinus is holoblastic and sympodial. The mushroom primordia are differentiated internally into gill, cap, and stipe hyphae while only l-2mm in diameter. At this stage they look superficially like sclerotial …

Buoyancy Changes As Related To Respiratory Behavior In An Amphibious Snail, Pomacea Urceus (Müller), From Venezuela, Kathleen A. Burky, Albert J. Burky

Biology Faculty Publications

The ampullariid Pomacea urceus uses its ctenidium and lung in respiration. The snails reach the surface with their siphon and ventilate their lung by withdrawing their head-foot in a pumping action. The lung gas also serves to change the buoyancy of the snails. After a ventilation a snail does not necessarily return to the same buoyancy level. They may achieve overall specific gravities of <1 to > 1 after a ventilation. They may remain submerged (s.g. > 1) or float (s.g.

The Effect Of Cv-1 Cell Geometry On The Ultraviolet Radiation Sensitivity Of Mammalian Cells In Vitro, Daniel J. Knauer

Masters Theses & Specialist Projects

The effect of cell geometry on the radiosensitivity to ultraviolet (UV) light has been studied in the CV-1 host cell-capacity system. In this system the macromolecular damage incurred by monolayers of cells cultures as the result of exposure to UV radiation is monitored by testing the ability of irradiated cells to support the replication of Herpes simplex virus. The target molecule has been shown to be DNA and is centrally located

Mammalian cells have an absolute requirement for Mg⁺⁺ and Ca⁺⁺ in order to remain attached to artificial substrata. To test the effect of cell geometry on UV …

Localization Of Neurosecretory Cells Within The Cerebral Ganglia Of Amphiphorus Imparispinosus Griffin, 1898 (Hoplonemertea) And Their Possible Regulatory Role In The Annual Reproductive Cycle, Gail H. Jacobson

Dissertations and Theses

Nemerteans are common invertebrates along coastlines in both temperate and arctic climates throughout the world. Amphiphorus imparispinosus is a carnivorous member of this phylum that is found abundantly in the intertidal areas of the Oregon coast. As in many other invertebrate phyla, endocrine integration within the nemerteans is thought to be accomplished by neurosecretory mechanisms. In Amphiphorus the presence of these neurosecretory systems has not been previously shown. This project was undertaken to determine if neurosecretory cells are present within the central nervous system of this species and. further, to examine seasonal changes in the number and appearance of these …

The Dispersal And Dispersion Patterns Of Hydra Fusca In A Limited Environment, Faith E. Ruffing

Dissertations and Theses

The dispersal and dispersion patterns of Hydra fusca were examined. Hydra were placed in petri dishes at various densities. The water in the dishes was swirled forcing the animals to the center. The location of each animal was marked at time intervals thereafter. Analyses of the dispersal rates and dispersion patterns were made. Hydra dispersed from a central release point at a non-random rate. There was rapid movement from the center followed by a minimal daily movement. This eventually resulted in a uniform dispersion pattern at high densities in a limited environment. There was a relationship between the ratio of …

Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoretic Studies Demonstrating Cytoplasmic Protein Components Unique To The Mycelial Phase And To The Yeast Phase Of Candida Albicans, Theresa Catherine Burgess

Biological Sciences Theses & Dissertations

Blastospore and mycelial cytoplasmic extracts from four strains of Candida albicans, varying in virulence, were compared by sodium dodecyl sulfate (SDS) polyacrylamide disc gel electrophoresis Cytoplasmic extracts were made by disrupting the cultures in a Braun homogenizer followed by ultracentrifugation. The supernatant fractions were then resolved on polyacrylamide gels containing SDS. Each of the preparations yielded a distinct pattern. There were mycelial specific and blastospore specific protein bands. There were more components resolved from the blastospore phase cytoplasmic extracts than from the mycelial phase cytoplasmic extracts. There were bands common to both phases. These results demonstrate protein components unique …

Patterns Of Proteins Synthesized In The R15 Neuron Of Aplysia: Temporal Studies And Evidence For Processing, F. Strumwasser, David L. Wilson

Biology Articles and Papers

The time-course of changes in the pattern of newly synthesized proteins in the R15 neuron of the parietovisceral ganglion of Aplysia californica has been studied at 14 degrees C. 5% polyacrylamide gels containing sodium dodecyl sulfate (SDS) have been used to separate newly synthesized (leucine-labeled) proteins from the neuron. We have demonstrated that the pattern of newly synthesized proteins from the R15 neuron does not change significantly if 5-h pulses of labeled leucine are given during the first 72 h of in vitro incubation of the excised ganglion. However, the level of leucine incorporation begins to decline somewhere between 17 …

Respiratory Metabolism And Energy Requirements Of Embryo, Larval And Juvenile Mountain Whitefish, Prosopium Williamsoni, Pokkavil Karunakara Rajagopal

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The upper optimum temperature for embryonic development of mountain whitefish is 6 C, and for the post yolk sac stage is 9 to 12 C. The need to know effects on all stages in the life cycle in cases of thermal pollution is demonstrated. Abnormalities caused by thermal pollution in hatched larvae were agape jaws, coloboma or fissure of the eye, monophthalmia or the presence of only one eye, monomicrophthalmia or the presence of one small eye and one case of twinning. High mortalities of eggs occurred at 9 C and higher.

Study of the energy expenditure, by the dry …

Histology Of The Small Intestine Of The Mink, Mustela Vison, Don Hurdman Bark Iii

All Master's Theses

This thesis was designed to provide a histological and histochemical survey of the small intestine of the mink, Mustela vison. These two objectives were achieved and it was found that the histology and enzyme activity in this animal is in most respects similar to other mammals. Exceptions to this are the absence of a cecum and an ileocecal valve and the absence of lactase activity in the small intestine.

Isolation And Characterization Of Two Bacteriophages Infecting The Myxobacterium Cytophaga Succinicans, Kenneth Wayne Lee

All Master's Theses

Two bacteriophages which infect the myxobacterium Cytophaga succinicans, strains 14 and 16, were isolated from fish hatchery effluent. Each was submitted to a detailed study. These phages have a limited host range, even within the genus Cytophaga. The turbid plaques formed by these phages are similar in appearance to those produced by coliphage Lambda, and average 0.85 mm in diameter. Both phages have a latent period of 195 minutes and a rise period of an additional 165 minutes. Phage 0CS-16 has a burst size of 137 and phage 0CS-14 has a burst of 45. Sodium ion at a concentration of …

The Effects Of The Cyclic 3'5'-Monophosphate Of Adenosine And Its Dibutyryl Derivative On The In-Vitro Immunization Of Mouse Spleen Cell Suspensions, James A. Blanks

Biological Sciences Theses & Dissertations

Abstract unavailable.

Molecular Weight Distribution Of Proteins Synthesized In Single, Identified Neurons Of Aplysia, David L. Wilson

Biology Articles and Papers

Parietovisceral ganglia from Aplysia californica were incubated in medium containing leucine-³H. Single, identified nerve cell somas were isolated from the ganglia, and their proteins extracted and separated by electrophoresis on 5% SDS-polyacrylamide gels. The distribution of total or newly synthesized proteins from the single neurons was determined by staining or slicing and liquid scintillation counting of the gels. Experiments showed that: (a) a number of proteins were being synthesized in abundance in the nerve cells; (b) different, identified neurons showed reproducibly different labeling patterns in the gels; (c) cells R2 and R15, …

The In Utero Development Of Mouse Embryos After Cultivation In Vitro From Two-Cell To Blastocyst In 17b - Estradiol Treated Medium, Harley J. Schneider Jr.

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead state University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Harley J. Schneider, Jr. on August 27, 1970.

A Balanced Salt Culture Medium For Halteria Grandinella, Yee Wan Mark

All-Inclusive List of Electronic Theses and Dissertations

The animals that the writer worked with liere the unicellular organisms called liaJ,tez:ia grandil.!iilJ.a• They belong to the Pl~lum Protozoa and are placed in the Class Ciliata and the Order Oligotrichia. llftltez'A gDJ}gini•U, like most protozoa, ll ve in waters but they seem to prefer qUiet pools, lakes, and ponds in which aqua i;;!c plants are growing and in which there is much organic matter.

The Chromosomes Of The Yeast Cell, Donald William Penn

Bachelors’ Theses

Saccharomyces cerevisiae has long been a fungus of economic importance in the baking and beverage fields. A large amount of research has been done on this yeast cytologically and biochemically. However, due to its small size and difficulty in staining, the cytology of the yeast cell remains somewhat in question. Chromosomes and division figures have been demonstrated by several workers, but there still remain sharp differences of opinion as to the nature of the nucleus. Recent work on the genetics of yeast has shown evidence of chromosome division similar to that occurring in higher plants and studies of linkage have …

The Development Of The Sporangium Of Aspidium (Cyrtomium) Falcatum, Rita Thorpe

Bachelors’ Theses

It is the purpose of the writer in this thesis, to describe the development of the sporangium of Aspidium (Cyrtomium) falcatum, and to observe some of the cytological features of the sporogenous cells.

Increased Fertility In Drosophila In The Heterozygote Of Two Allelomorphs Each Homozygous Infertile, M. M. Green, C. P. Oliver

Journal of the Minnesota Academy of Science

No abstract provided.

A Cytological Study Of The Intestinal Glandular Epithelium, Mary Judelle Zuiker

Bachelors’ Theses

Continuing from the stomach, the digestive tract becomes a smaller tube whose internal surface is much increased by means of folds--the plica circularis, the villi, and crypts of Lieberkühn. The plica (formerly known as valvulae conniventes) are really elevations of the submucosa covered on both sides by the entire mucous membrane-villi, glands, and muscularis mucosae. The mucosa membrane, or tunica mucosa, is lined by simple columnar epithelium in which many goblet cells are present, particularly in the deeper folds. The basement membrane, as well as the muscularis mucosa, are identical with those of the stomach.

The Mammalian Erythrocyte, H.J. Werner

Bachelors’ Theses

An investigation of bibliographies shows that the red corpuscles of the blood have been studied very extensively, in fact, wore so than any cell in the mammalian body. The study has progressed vigorously and from varied points of view.

Staining Techniques In The Study Of The Blood, Donald E. Winnik

Bachelors’ Theses

Since the invention of the microscope, the study of the blood and its constituents has been a major interests of histologists and hematologists. The vast amount of material written about the subject could not have been accomplished without the development and use of staining techniques which made possible a more precise observation of the nature of the blood. At the present time there are many procedures and stains which vary greatly in their application and use. It is the purpose of this thesis to review these many methods and catalogue them according to their application in the blood techniques.

The Effect Of Potassium-Iodide On The Embryological Development Of The Chick With Special Reference To The Growth Of The Long Bones., David M. Polot 1904-1978

Electronic Theses and Dissertations

No abstract provided.

Organization And Polarity Of Protoplasm, John P. Munson

All Faculty Scholarship for the College of the Sciences

In higher forms of life, we usually concede the presence of an organ specialized for the performance of a function. In these higher forms coordination of organs, which entails a regular sequence in their functions, is readily made out; and it seems a mere platitude to say, of these, that organization determines the functions of the organism. To what extent can functions exist in the absence of organization; does the one precede the other; or have function and organization developed simultaneously, out of matter devoid of both structure and function? In my studies on the cell, I have kept these …

Spermatogenesis Of The Butterfly, Papilio Rutulus, John P. Munson

All Faculty Scholarship for the College of the Sciences

For a number of years I have been studying the spermatogenesis of Papilio rutulus (pl. 12, fig. 1) not ,with any one single problem especially in view, but with the aim of ascertaining the entire life history of its sperm cell, its various phases of development, its mode of growth and differentiation, the history of its several parts, and its relation to the organism.

Researches On The Oogenesis Of The Tortoise, Clemmys Marmorata, John P. Munson

Biology Faculty Scholarship

No abstract available.