Open Access. Powered by Scholars. Published by Universities.®
- Discipline
-
- Physical Sciences and Mathematics (2)
- Applied Mathematics (1)
- Biochemistry, Biophysics, and Structural Biology (1)
- Biodiversity (1)
- Chemistry (1)
-
- Diseases (1)
- Ecology and Evolutionary Biology (1)
- Genetics and Genomics (1)
- Immunology and Infectious Disease (1)
- Marine Biology (1)
- Medicine and Health Sciences (1)
- Microbiology (1)
- Molecular and Cellular Neuroscience (1)
- Nervous System Diseases (1)
- Neuroscience and Neurobiology (1)
- Other Physical Sciences and Mathematics (1)
- Institution
- Publication Year
- Publication
- Publication Type
Articles 1 - 18 of 18
Full-Text Articles in Biology
Isolation Of Aged Mouse Primary Microglia As A Model System For Alzheimer’S Disease Research, Michael Landis
Isolation Of Aged Mouse Primary Microglia As A Model System For Alzheimer’S Disease Research, Michael Landis
Biology Honors Papers
Microglia and their role as the immune cells of the central nervous system are an emerging area of interest within Alzheimer’s research, particularly as they have shown in a benevolent and malevolent cellular context. Models of Alzheimer’s disease are very light in studying microglia, so in this study a model of microglia isolated from aged mice is established in order to study the phagocytic activity and protein expression of microglia in response to Amyloid Beta. The cells were isolated from aged mice and cultured before being used to confirm cellular identity, as well as to measure phagocytic activity. This study …
Characterization Of A Stenotrophomonas Maltophilia Bacteriophage, Edward Eshoo
Characterization Of A Stenotrophomonas Maltophilia Bacteriophage, Edward Eshoo
College of Science and Health Theses and Dissertations
Stenotrophomonas maltophilia is a ubiquitous Gram-negative, multidrug resistant, opportunistic bacterial pathogen that causes various infections in humans. Recently, the use of bacteriophages as therapeutic agents, has gained interest as an alternative to traditional antibiotics. This thesis describes the isolation, purification, and characterization of S. maltophilia bacteriophage Bfi2 and discusses its activity against related, and often co-isolated, bacterial pathogens. Amplification of the phage resulted in clear, well-defined plaques and a titer of 1.73 ± 0.38 x 1011 PFU/ml. Bfi2 demonstrated the ability to lyse 55% of the S. maltophilia strains tested, suggesting that it has a moderate host range. However, the …
(R1507) Mathematical Modeling And Analysis Of Seqiahr Model: Impact Of Quarantine And Isolation On Covid-19, Manoj Kumar Singh, . Anjali
(R1507) Mathematical Modeling And Analysis Of Seqiahr Model: Impact Of Quarantine And Isolation On Covid-19, Manoj Kumar Singh, . Anjali
Applications and Applied Mathematics: An International Journal (AAM)
At the moment in time, an outbreak of COVID-19 is transmitting on from human to human. Different parts have different quality of life (e.g., India compared to Russia), which implies the impact varies in each part of the world. Although clinical vaccines are available to cure, the question is how to minimize the spread without considering the vaccine. In this paper, via a mathematical model, the transmission dynamics of novel coronavirus with quarantine and isolation facilities have been proposed. The examination of the proposed model is set in motion with the boundedness and positivity of the solution, sole disease-free equilibrium, …
Listeria Occurrence In Poultry Flocks: Detection And Potential Implications, Michael J. Rothrock Jr., Morgan L. Davis, Aude Locatelli, Aaron Bodie, Tori G. Mcintosh, Janet R. Donaldson, Steven C. Ricke
Listeria Occurrence In Poultry Flocks: Detection And Potential Implications, Michael J. Rothrock Jr., Morgan L. Davis, Aude Locatelli, Aaron Bodie, Tori G. Mcintosh, Janet R. Donaldson, Steven C. Ricke
Faculty Publications
Foodborne pathogens such as Salmonella, Campylobacter, Escherichia coli, and Listeria are a major concern within the food industry due to their pathogenic potential to cause infection. Of these, Listeria monocytogenes, possesses a high mortality rate (approximately 20%) and is considered one of the most dangerous foodborne pathogens. Although the usual reservoirs for Listeria transmission have been extensively studied, little is known about the relationship between Listeria and live poultry production. Sporadic and isolated cases of listeriosis have been attributed to poultry production and Listeria spp. have been isolated from all stages of poultry production and processing. Farm studies …
A Comparative Study Of Isolation In Headwater Fishes, Bjorn Victor Schmidt
A Comparative Study Of Isolation In Headwater Fishes, Bjorn Victor Schmidt
Dissertations
Headwater resident fishes may be prone to a high rate of population fragmentation within river networks because large streams have habitat conditions outside of their preferred ecological niche and may limit gene flow in the dendritic ecological network. To investigate patterns of population structure, asymmetrical gene flow, and influences on genetic distance and isolation from connecting habitat pathways, species specific ecological traits, and basin scale characteristics, a multi-species, multi-regional study was performed. Six headwater species of fish from four taxonomic groupings were sampled for genetic material in three regions of paired neighbor drainages and then genotyped for eight microsatellite loci. …
Bears In Pakistan: Distribution, Population Biology And Human Conflicts, Fakhar -I- Abbas, Zahid Iqbal Bhatti, Jibran Haider, Afsar Mian
Bears In Pakistan: Distribution, Population Biology And Human Conflicts, Fakhar -I- Abbas, Zahid Iqbal Bhatti, Jibran Haider, Afsar Mian
Journal of Bioresource Management
We conducted questionnaire based interviews (n = 1873) of respondents coming from 258 localities about bear tracts in northern parts of Pakistan in 2012-2014 to study Himalyan brown (U. arctos isalbellinus) and Himalayan black (U. t. laniger) bears. Brown bears were more frequent in northern latitudes (northern Chitral, Ghizer, Gilgit and Skardu), while black bears were widely distributed in southern latitudes (Battagram). Both brown and black bears are present in central latitudes (Astor, Diamir, Kohistan and Mansehra). We identified 34 populations of brown bears; a large population in the Deosai Plateau and small to very small …
The Relative Influence Of Habitat Amount And Configuration On Genetic Structure Across Multiple Spatial Scales, Katie L Millette, Nusha Keyghobadi
The Relative Influence Of Habitat Amount And Configuration On Genetic Structure Across Multiple Spatial Scales, Katie L Millette, Nusha Keyghobadi
Biology Publications
Despite strong interest in understanding how habitat spatial structure shapes the genetics of populations, the relative importance of habitat amount and configuration for patterns of genetic differentiation remains largely unexplored in empirical systems. In this study, we evaluate the relative influence of, and interactions among, the amount of habitat and aspects of its spatial configuration on genetic differentiation in the pitcher plant midge, Metriocnemus knabi. Larvae of this species are found exclusively within the water-filled leaves of pitcher plants (Sarracenia purpurea) in a system that is naturally patchy at multiple spatial scales (i.e., leaf, plant, cluster, peatland). Using generalized linear …
Intraspecific Variation In Carapace Morphology Among Fiddler Crabs (Genus Uca) From The Atlantic Coast Of Brazil, Kelsey R. Hampton, Melanie J. Hopkins, John C. Mcnamara, Carl L. Thurman
Intraspecific Variation In Carapace Morphology Among Fiddler Crabs (Genus Uca) From The Atlantic Coast Of Brazil, Kelsey R. Hampton, Melanie J. Hopkins, John C. Mcnamara, Carl L. Thurman
Faculty Publications
Isolation due to geographical barriers should promote genetic and morphological divergence among populations. Marine currents flowing in opposing directions along landmasses can constitute barriers that isolate populations dependent upon aquatic dispersal. The distribution of fiddler crabs (genus Uca) is regulated primarily by the oceanic transport of their planktonic larvae and by available adult habitat. Along the Brazilian coast of eastern South America, the flow of 2 major oceanic currents separates northern from southern Uca populations, which may promote intraspecific divergence in ‘trans-Brazilian’ species. Populations of 10 Uca species were sampled at 64 locations north and south of the Ponta …
Relationships Between Isolating Land Use And Amphibian Populations In Sub-Boreal Peatlands Of The Midwestern United States, Jeana Rose Albers
Relationships Between Isolating Land Use And Amphibian Populations In Sub-Boreal Peatlands Of The Midwestern United States, Jeana Rose Albers
All Graduate Theses, Dissertations, and Other Capstone Projects
Successful conservation efforts of amphibians depend on the knowledge of habitat preferences because the biggest threat to amphibian populations is considered to be habitat loss. Sub-boreal peatlands in the Midwest may be a refuge for amphibian populations, but little is known about the flora and fauna of these peatlands. My study examined amphibian species richness and species diversity in 17 sub-boreal peatlands in Minnesota and Wisconsin in 2011 and 2012. I assessed the relationship between land use of lands adjacent to peatlands and use of those peatlands by amphibian populations at three spatial scales by examining landscapes surrounding peatlands in …
Isolation Of Primary Cilia By Shear Force, Kimberly A. P. Mitchell
Isolation Of Primary Cilia By Shear Force, Kimberly A. P. Mitchell
Faculty Publications and Presentations
The cell’s primary cilium is both a mechanical and chemical sensor involved in many signaling pathways. In order to ascertain protein enrichment in the primary cilium or study sub-ciliary localization of various proteins, it is advantageous to remove the primary cilium from the cell body. The protocol described here gives detailed instructions on purifying primary cilia by separating them from the cell body using shear force. This simple technique avoids using harsh purification conditions that may affect signaling proteins in the cilium or cause the ciliary membrane to disintegrate. In addition, as the cell body remains mostly intact, contamination of …
The Influence Of Habitat Composition And Configuration On The Genetic Structure Of The Pitcher Plant Midge, Katie Millette
The Influence Of Habitat Composition And Configuration On The Genetic Structure Of The Pitcher Plant Midge, Katie Millette
Electronic Thesis and Dissertation Repository
A major question in landscape genetics is how habitat structure influences spatial patterns of genetic differentiation. In this study, I evaluate the relative importance and effects of aspects of habitat composition (habitat amount) and configuration (patch size and isolation) on the spatial genetic structure of the pitcher plant midge, Metriocnemus knabi, whose larvae are found exclusively within the water-filled leaves of pitcher plants (Sarracenia purpurea) in a system that is naturally patchy at multiple spatial scales (i.e., leaf, plant, cluster, bog). I estimated genetic differentiation (FST) among leaves, plants, and clusters using 11 microsatellite …
Isolation And Identification Of Trichoderma Species From Different Habitats And Their Use For Bioconversion Of Solid Waste, Ahsanur Rahman, Most. Ferdousi Begum, Matiur Rahman, M. A. Bari, G.N.M. Illias, M. Firoz Alam
Isolation And Identification Of Trichoderma Species From Different Habitats And Their Use For Bioconversion Of Solid Waste, Ahsanur Rahman, Most. Ferdousi Begum, Matiur Rahman, M. A. Bari, G.N.M. Illias, M. Firoz Alam
Turkish Journal of Biology
The native population of Trichoderma species collected from different habitats was quantified and characterised. The identified strains are T. harzianum (IMI-392432, 392433, 392434); T. pseudokoningii (IMI-392431) and T. virens (IMI-392430). Out of five strains, T. harzianum was the most common in all of the habitats. Colony forming units (cfu's) of Trichoderma species varied significantly (P = 0.05) in different habitats and were positively correlated with the physico-chemical characteristics of the habitat. Trichoderma was found to have adapted to an acidic habitat. In testing for an efficient bioconversion agent, T. harzianum (IMI-392432) was found to be the most effective in kitchen …
Methods For The Isolation Of Sensory And Primary Cilia—An Overview, Kimberly A. P. Mitchell, Gabor Szabo, Angela De S. Otero
Methods For The Isolation Of Sensory And Primary Cilia—An Overview, Kimberly A. P. Mitchell, Gabor Szabo, Angela De S. Otero
Faculty Publications and Presentations
Detailed proteomic analyses of mammalian olfactory and rod photoreceptor sensory cilia are now available, providing an inventory of resident ciliary proteins and laying the foundation for future studies of developmental and spatio-temporal changes in the composition of sensory cilia. Cilia purification methods that were elaborated and perfected over several decades were essential for these advances. In contrast, the proteome of primary cilia is yet to be established, because purification procedures for this organelle have been developed only recently. In this chapter we review current techniques for the purification of olfactory and photoreceptor cilia, and evaluate methods designed for the selective …
Characterization Of Rhizobium Sp. Isolated From Bean, Çi̇ğdem Küçük, Meri̇h Kivanç, Engi̇n Kinaci
Characterization Of Rhizobium Sp. Isolated From Bean, Çi̇ğdem Küçük, Meri̇h Kivanç, Engi̇n Kinaci
Turkish Journal of Biology
Thirty nodule isolates from bean (Phaseolus vulgaris L.) grown in Eskişehir were studied for their physiological and biochemical characteristics. Most isolates produced abundant extracellular polysaccharides, tolerated high salt concentration (5% NaCl), grew at a temperature of 42 °C, and synthesized melanin. They were able to grow at pHs ranging from 3.5 to 9.0. The majority of the isolates showed an intrinsic resistance to the antibiotics chloramphenicol (20 and 50 µg ml^{-1}), erythromycin (30 µg ml^{-1}), kanamycin (10 µg ml^{-1}), and streptomycin (40, 80, and 100 µg ml^{-1}). Plasmid DNA profiles of the isolates were identified and it was determined that …
Properties Of The Rhodopseudomonas Palustrisstrains Isolated From An Alkaline Lake In Turkey, Gönül Çeti̇nkaya Dönmez, Ayten Öztürk, Lütfü Çakmakçi
Properties Of The Rhodopseudomonas Palustrisstrains Isolated From An Alkaline Lake In Turkey, Gönül Çeti̇nkaya Dönmez, Ayten Öztürk, Lütfü Çakmakçi
Turkish Journal of Biology
31 photoheterotrophic bacteria were isolated from Lake Aksehir sediment. Identification of the strains was based on morphological properties, motility and pigment composition, and also physiological properties such as carbon utilization and ability to respire anaerobically and aerobically in the dark. All strains were identified as Rhodopseudomonas palustris, but some strains were distinct from R. palustris with regard to vitamin requirement, malate utilization and aerobic growth in the dark. Until now, R. palustris had not been reported from an alkaline lake, and the strains growing alkaline pH (9) could be new strains of R. palustris.
Isolation And Abundance Of Unicellularcyanobacteria From Mosquito Development Sites, Gönül Çeti̇nkaya Dönmez, Ayşe Elmaci, Olcay Obali, Ayten Öztürk, Lütfü Çakmakçi
Isolation And Abundance Of Unicellularcyanobacteria From Mosquito Development Sites, Gönül Çeti̇nkaya Dönmez, Ayşe Elmaci, Olcay Obali, Ayten Öztürk, Lütfü Çakmakçi
Turkish Journal of Biology
The genus composition of cyanobacteria (particularly Chroococcus sp.) and their abundance in mosquito breeding sites at Lake Mogan (Ankara, Turkey) increased from August to September. Six unicellular cyanobacteria that had different morphological properties were isolated and succesfully cultivated. According to the mode of division, these six strain were classified as the represantatives of subgroup 1 (order Choroococcales). One of the isolates was identified as Synechococcus sp. and one as Gloeocapsa sp., and four were identified as Synechocystic sp. One of the four Synechocystis spp. was shown to be capable of heterotrophic growth.
Investigation On The Isolation And Identification Of The Listeria Species In The White And Civil Cheeses Purchased From Erzurum Region, A. Aydan Kara, Ömer Faruk Algur, Mükerrem Kaya
Investigation On The Isolation And Identification Of The Listeria Species In The White And Civil Cheeses Purchased From Erzurum Region, A. Aydan Kara, Ömer Faruk Algur, Mükerrem Kaya
Turkish Journal of Biology
In this study, 34 white cheeses and 16 civil (local name) cheeses purchased from markets and shops in Erzurum during the period of January 1995-April 1996 were analysed. The samples were streaked directly onto LSA and Palcam Agar media in Petri Plates and incubated at 37C° for 48 hours. After the incubation, the growing colonies on LSA-agar plates were examined by Henry's Modified IIIumination Method while collonies on Palcam agar were examined by naked eye. Typical colonies were selected and used for several biochemical tests. As a result, it was found that only one sample (2.94%) of the white cheeses …
Characterization And Genetic Analysis Of A Pigmentation Mutant Of Neurospora Crassa, Craig West Banks
Characterization And Genetic Analysis Of A Pigmentation Mutant Of Neurospora Crassa, Craig West Banks
Legacy ETDs
The mutant overaccumulator of carotenoids, ovc. was obtained from the Fungal Genetics Stock Center in Kansas City, Kansas. This mutant had been isolated following UV irradiation of the colonial mutant col-4 and mapped to Linkage Group IV (R. Harding, 1984 Neurospora Newsletter 34: 23-25). Examination of ovc in our laboratory revealed that in addition to having increased pigmentation, the mutant was osmotic sensitive, failing to grow on 6% NaCl. Morphology of ovc closely resembles that of cut, another osmotic sensitive mutant in LG IV. A cross of ovc to the osmotic sensitive mutant cut failed to produce …