Biology Commons^™

Mixed Method Analysis Of Undergraduate Student Interpretation Of Different Phylogenetic Trees, Faith Frings

USF Tampa Graduate Theses and Dissertations

Phylogenetic trees are common tools used to visualize evolutionary concepts such as historical patterns of ancestry, divergence of species, and descent of species. However, students have misconceptions when reading these abstract diagrams. The purpose of this study was to compare student performance and evolutionary thinking when using two styles of phylogenetic trees: cladograms and phylograms. The study also assessed the validity of a hierarchal theoretical framework evaluating student phylogenetic tree interpretation. Introductory biology students from two research universities were assigned to two groups, one solely given assessments with phylograms, and one solely given assessments with cladograms. One-on-one student interviews were …

The Mitogenome Relationships And Phylogeography Of Barn Swallows (Hirundo Rustica), Gianluca Lombardo, Nicola Rambaldi Migliore, Giulia Colombo, Marco Rosario Capodiferro, Giulio Formenti, Manuela Caprioli, Elisabetta Moroni, Leonardo Caporali, Hovirag Lancioni, Simona Secomandi, Cristina Cereda, Valerio Carelli, Lauren Gillespie, Yang Liu, Yosef Kiat, Alfonso Marzal, Cosme López-Calderón, Javier Balbontín, Timothy Mousseau, Piotr Matyjasiak, Anders Pape Møller, Et. Al.

Faculty Publications

The barn swallow (Hirundo rustica) poses a number of fascinating scientific questions, including the taxonomic status of postulated subspecies. Here, we obtained and assessed the sequence variation of 411 complete mitogenomes, mainly from the European H. r. rustica, but other subspecies as well. In almost every case, we observed subspecies-specific haplogroups, which we employed together with estimated radiation times to postulate a model for the geographical and temporal worldwide spread of the species. The female barn swallow carrying the Hirundo rustica ancestral mitogenome left Africa (or its vicinity) around 280 thousand years ago (kya), and her descendants …

Genetically Explicit Model May Explain Multigenerational Control Of Emergent Turing Patterns In Hybrid Mimulus, Emily Simmons

Biology and Medicine Through Mathematics Conference

No abstract provided.

Evolution Of Floral Microbes And The Resulting Effects On Pollinator Preference, Hailey Hatch

Undergraduate Honors Capstone Projects

Floral microbes are an overlooked aspect of the extended floral phenotype. Through altering floral nectar chemistry, they can mediate interactions between flowers, pollinators, and other floral microbes, with significant implications for plant and pollinator health. Interactions between floral microbes and pollinators are critically important to understand, as pollinators provide important ecosystem services in both natural and agriculture systems. Here, I explored how floral nectar traits affected both evolution and competition within the floral yeast Metschnikowia reukaufii, the floral bacterium Bacillus subtilis, and other microbes isolated from Brassica rapa nectar, an important plant model system and oilseed crop. To …

The Evolution Of Neurosensory Structures: Evaluating Ecological Drivers Of Brain And Eye Size Variation, Kaitlyn J. Howell

Biology Dissertations - Archive

Neurosensory structures (i.e., brain and eye size) vary extensively across taxa. The evolution of large neurosensory structures has long been an area of interest for scientists, but focus has largely been placed on macroevolutionary patterns at or above the species level. Few studies have investigated the role contrasting ecological factors play in the evolution of such structures within species. My dissertation examines 1) how divergent ecological pressures, particularly predation and competition, drive evolutionary shifts in brain and eye size within species and across sexes, and 2) why such variation evolves, particularly if larger brains drive shifts in behavior and fitness. …

Describing Bacterial Mutagenic Hotspots And Signatures Along With The Factors That Drive Them, Carmen Vallin

UNLV Theses, Dissertations, Professional Papers, and Capstones

In my dissertation work, I investigated bacterial mutagenesis. I asked: where do mutations occur in the bacterial genome? What types of mutations occur? What factors promote or prevent mutations? And lastly, how do the answers to these questions change depending on the bacterial life cycle (When)? I used Bacillus subtilis, a Gram-positive bacterium belonging to the phylum Firmicutes, and Escherichia coli, a Gram-negative bacterium belonging to the phylum Proteobacteria, to answer these questions. B. subtilis and E. coli are model systems that have been used to gain insights into fundamental processes of mutations and evolution for nearly ~8 decades. Here, …

In-Class Evolution Discussions Change Bible Interpretation, Jessica Abele

Library/Life Sciences Undergraduate Poster Competition 2022

Conflict between religion and evolution is a major barrier for religious biology students. Educators have been working to increase evolution acceptance by using a variety of methods (e.g., increasing evolution knowledge, addressing creationism in class, etc.) with little success. However, recent research shows using a reconciliatory approach to evolution and religion increases evolution acceptance while overall religiosity is unaffected (Lindsay et al., 2019).

Phylogenomic Discordance Suggests Polytomies Along The Backbone Of The Large Genus Solanum, Edeline Gagnon, Rebeccca Hilgenhof, Andrés Orejuela, Angela J. Mcdonnell, Gaurav Sablok, Xavier Aubriot, Leandro Giacomin, Yuri Gouvêa, Thamyris Bragionis, João Renato Stehmann, Lynn Bohs, Steven Dodsworth, Christopher T. Martine, Péter Poczai, Sandra Knapp, Tiina Särkinen

Faculty Journal Articles

Premise of the study

Evolutionary studies require solid phylogenetic frameworks, but increased volumes of phylogenomic data have revealed incongruent topologies among gene trees in many organisms both between and within genomes. Some of these incongruences indicate polytomies that may remain impossible to resolve. Here we investigate the degree of gene-tree discordance in Solanum, one of the largest flowering plant genera that includes the cultivated potato, tomato, and eggplant, as well as 24 minor crop plants.

Methods

A densely sampled species-level phylogeny of Solanum is built using unpublished and publicly available Sanger sequences comprising 60% of all accepted species (742 spp.) …

Why Sequence All Eukaryotes?, Mark Blaxter, John M. Archibald, Anna K. Childers, Jonathan A. Coddington, Keith A. Crandall, Federica Di Palma, Richard Durbin, Scott V. Edwards, Jennifer A.M. Graves, Kevin J. Hackett, Neil Hall, Erich D. Jarvis, Rebecca N. Johnson, Elinor K. Karlsson, W. John Kress, Shigehiro Kuraku, Mara K. N. Lawniczak, Kerstin Lindblad-Toh, Jose V. Lopez, Nancy A. Moran, Gene E. Robinson, Oliver A. Ryder, Beth Shapiro, Pamela S. Soltis, Tandy Warnow, Guojie Zhang, Harris A. Lewin

Biology Faculty Articles

Life on Earth has evolved from initial simplicity to the astounding complexity we experience today. Bacteria and archaea have largely excelled in metabolic diversification, but eukaryotes additionally display abundant morphological innovation. How have these innovations come about and what constraints are there on the origins of novelty and the continuing maintenance of biodiversity on Earth? The history of life and the code for the working parts of cells and systems are written in the genome. The Earth BioGenome Project has proposed that the genomes of all extant, named eukaryotes—about 2 million species—should be sequenced to high quality to produce a …

Geographic Range Size As A Predictor Of Dispersal-Dependent Behavioral Traits In Two Clades Of A Terrestrial Salamander, Teah Evers

Masters Theses

Animal movement has the potential to affect diverse processes within ecology and evolution including range expansion, gene flow, adaptation, and speciation. Two aspects of animal personality that are germane to dispersal are exploratory and aggressive behavior. These behavioral categories may represent a trade-off such that energy invested in territorial defense leaves little energy for movement and dispersal. The Eastern Red-backed Salamander (Plethodon cinereus) is a wide ranging, dispersal limited, terrestrial salamander with well documented phylogeographic divisions. I examined dispersal-relevant behavioral traits within two clades of P. cinereus with disparate geographic ranges. The Northern Clade (NC) has a range extending from …

(Non)Parallel Developmental Mechanisms In Vertebrate Appendage Reduction And Loss, Samantha Swank, Thomas Sanger, Yoel E. Stuart

Biology: Faculty Publications and Other Works

Appendages have been reduced or lost hundreds of times during vertebrate evolution. This phenotypic convergence may be underlain by shared or different molecular mechanisms in distantly related vertebrate clades. To investigate, we reviewed the developmental and evolutionary literature of appendage reduction and loss in more than a dozen vertebrate genera from fish to mammals. We found that appendage reduction and loss was nearly always driven by modified gene expression as opposed to changes in coding sequences. Moreover, expression of the same genes was repeatedly modified across vertebrate taxa. However, the specific mechanisms by which expression was modified were rarely shared. …

Resource Allocation And Phenotypic Plasticity Of Simultaneous Hermaphroditic Turtle Barnacles (Chelonibia Testudinaria), Kevin C. Cash

All HCAS Student Capstones, Theses, and Dissertations

This research addresses the knowledge gap of phenotypic plasticity in a commonly found and important species of epizoic barnacle, Chelonibia testudinaria. Limited research has been published regarding how phenotypic expression is mediated the spatial distribution of barnacles on a mobile host. To investigate this potential relationship, barnacles were collected from the backs of turtles along the beaches of Fort Lauderdale Florida. These barnacles were assessed for various phenotypic traits as well as their corresponding spatial distribution on the turtle carapace. Barnacles were safely removed from the carapace using a chisel before their preservation in ethanol. Barnacles were then numbered …

Reproductive Aging In Caenorhabditis Elegans: From Molecules To Ecology, Andrea Scharf, Franziska Pohl, Brian M. Egan, Zuzana Kocsisova, Kerry Kornfeld

Biological Sciences Faculty Research & Creative Works

Aging animals display a broad range of progressive degenerative changes, and one of the most fascinating is the decline of female reproductive function. In the model organism Caenorhabditis elegans, hermaphrodites reach a peak of progeny production on day 2 of adulthood and then display a rapid decline; progeny production typically ends by day 8 of adulthood. Since animals typically survive until day 15 of adulthood, there is a substantial post reproductive lifespan. Here we review the molecular and cellular changes that occur during reproductive aging, including reductions in stem cell number and activity, slowing meiotic progression, diminished Notch signaling, and …

Phenotype Bias Determines How Natural Rna Structures Occupy The Morphospace Of All Possible Shapes, Fatme Ghaddar, Dr Kamaludin Dingle, Dr Petr Sulc, Prof Ard A. Louis

Undergraduate Research Symposium

The relative prominence of developmental bias versus natural selection is a long standing controversy in evolutionary biology. Here we demonstrate quantitatively that developmental bias is the primary explanation for the occupation of the morphospace of RNA secondary structure (SS) shapes. By using the RNAshapes method to define coarse-grained SS classes, we can measure the frequencies that non-coding RNA SS shapes appear in nature. Our main findings are firstly that only the most frequent structures appear in nature; the vast majority of possible structures in the morphospace have not yet been explored. Secondly, and perhaps more surprisingly, these frequencies are accurately …

Phylogenomics And Population History Of Cichlid And Live-Bearing Fish Species In Lowland Neotropical Rivers, Konrad Taube

College of Science and Health Theses and Dissertations

Within Middle America, cichlids and poeciliids account for more than half of the fish biodiversity. This richness in fish fauna highlights the complexity of Middle American biogeography: no other continental area on earth contains within its range the unparalleled abundance of secondary freshwater fish species (fish that can tolerate both saltwater and freshwater). Research into the biogeography of widely distributed Middle American freshwater fish is essential to understanding this unique region.

Three species of freshwater fishes (Belonesox belizanus – Pike killifish, Vieja maculicauda – Black belt cichlid, and Gambusia nicaraguensis – Nicaraguan mosquitofish) are widely distributed across rivers on …

The Evolutionary History Of Camelina Crantz (Brassicaceae) And Domestication Of The Biofuel Crop, C. Sativa (L.) Crantz, Jordan Brock

Arts & Sciences Graduate Student Theses and Dissertations

The mustard family, Brassicaceae, is a large and economically important group of plants comprising ~350 genera and ~4060 species. Within the family, one genus, Camelina, has been neglected and nearly forgotten until recent decades. This genus comprises between 7 – 9 species, and includes Camelina sativa, an ancient oilseed crop cultivated for thousands of years in Europe. An allohexaploid, C. sativa contains high levels of omega-3 fatty acids and a seed oil composition amenable for production of aviation jet fuel. With a short generation time and ability to be cultivated on marginal soils with few inputs, C. sativa is set …

The Evolving Mammalian 3d Epigenome, Mayank Choudhary Nishikant Choudhary

Arts & Sciences Graduate Student Theses and Dissertations

One of life’s greatest mysteries is how a 6ft long-strand of DNA (our genome) fits inside the nucleus of a cell that’s less than a tenth of the width of a strand of hair. Adding to that, this genome has functioned faithfully with high fidelity over hundreds of millions of years of evolution is simply mind boggling. Moreover, within our genomes, we find millions of copies of strange genetic hitchhikers that were once thought to be purely self-seeking and parasitic. Critical advances in technology, assay development, sequencing, and computation have brought us today at an opportune moment in space and …

The Evolution Of Sex Differences In Mandrills (Mandrillus Sphinx): Micro- And Macroevolution, Jerred Klint Schafer

Legacy Theses & Dissertations (2009 - 2024)

Primates show diverse patterns of adaptive color and body size dimorphism produced by inter- and intrasexual selection. However, the specific microevolutionary processes that produce variation in secondary sexual characteristics remain largely unexplored in primates. Furthermore, sexual conflict theory predicts that female and male secondary sexual traits can coevolve in an antagonistic manner and promote speciation. This dissertation explores the microevolution of secondary sexual characteristics in mandrills (Mandrillus sphinx) and the macroevolution of these characters in anthropoid primates. I address the microevolution of mandrill facial coloration and body mass by estimating the heritability, phenotypic selection, and genetic evolution of these traits …

Individual Quality And Phenology Mediate The Effect Of Radioactive Contamination On Body Temperature In Chernobyl Barn Swallows, Zbyszek Boratynski, Timothy A. Mousseau, Anders Pape Moller

Faculty Publications

Anthropogenic stressors, such as radioactive contaminants released from the Chernobyl and Fukushima Daiichi accidents, deteriorate ecological and evolutionary processes, as evidence for damaging effects of radioactive contamination on wildlife is accumulating. Yet little is known about physiological traits of animals inhabiting contaminated areas, and how those are affected by individual quality and phenology. We investigated variation in body temperature of wild barn swallows, Hirundo rustica, exposed to radioactive contamination from the Chernobyl accident in Ukraine and Belarus. We tested whether exposure to variable levels of radioactive contamination modified core body temperature of birds, and whether individual and phenological characteristics modulated …

Evolution Of Targeted Therapy Resistance In Eml4-Alk Positive Non-Small Cell Lung Cancer, Robert Vander Velde

USF Tampa Graduate Theses and Dissertations

Targeted therapies have emerged as potent treatments that lead to the remission of many tumors. However, they rarely cure cancers in advanced, metastatic settings. This is due to the evolution of resistance, which in turn can be ascribed to the survival of small subpopulations of tolerant and/or resistant cells. Here we investigated the evolution of resistance to EML4-ALK inhibitors in non-small cell lung cancer (NSCLC) and demonstrated that resistance evolves gradually, from unique pre-treatment sub-populations, as multiple resistance mechanisms accumulate in a Darwinian fashion. Despite accumulating multiple changes, cells evolved, in parallel, toward similar inhibitor specific phenotypes. Evolving cells have …

Genomic Gc Content Drifts Downward In Most Bacterial Genomes, Ely Bert

Faculty Publications

In every kingdom of life, GC->AT transitions occur more frequently than any other type of mutation due to the spontaneous deamination of cytidine. In eukaryotic genomes, this slow loss of GC base pairs is counteracted by biased gene conversion which increases genomic GC content as part of the recombination process. However, this type of biased gene conversion has not been observed in bacterial genomes, so we hypothesized that GC->AT transitions cause a reduction of genomic GC content in prokaryotic genomes on an evolutionary time scale. To test this hypothesis, we used a phylogenetic approach to analyze triplets of …

Functional Genomics Reveals The Mechanisms And Evolution Of Extreme Physiological Adaptations In Snakes, Blair William Perry

Biology Dissertations - Archive

Previous studies examining post-feeding organ regeneration in the Burmese python (Python molurusbivittatus) have identified thousands of genes that are significantly differentially regulated during this process. However, substantial gaps remain in our understanding of coherent mechanisms and specific growth pathways that underlie these rapid and extensive shifts in organ form and function. Here we addressed these gaps by comparing gene expression in the Burmese python heart, liver, kidney, and small intestine across pre- and post-feeding time points (fasted, one day post-feeding, and four days post-feeding),and by conducting detailed analyses of molecular pathways and predictions of upstream regulatory molecules across these organ …

On The Rocks: Changes In Substrate Alter Antipredator Behavior In Benthic Stream Fish, Sarah White

Graduate Theses/Dissertations

Natural selection should strongly favor characteristics that make prey difficult for predators to distinguish from the background, including both morphological and behavioral crypsis. The Rainbow Darter (Etheostoma caeruleum) is a small, cryptic, benthic fish that inhabits gravel-bottomed streams and is preyed upon by predators such as the benthic Knobfin Sculpin (Cottus immaculatus) and the pelagic Longear Sunfish (Lepomis megalotis). In three experiments, I tested whether the behavior of darters was influenced by the opportunity for crypticity offered by their substrate and by the presence of cues from benthic and pelagic predators. First, darters in …

Evolution And Development Of Staminodes In Paronychia (Caryophyllaceae), Andrea D. Appleton

Honors College Theses

Staminodes are infertile stamens that have evolved numerous times in flowering plants and exhibit a vast array of forms and functions. Variation in staminodes suggests that numerous evolutionary processes underlie their origins, but to understand their how and why they evolved, comparative studies are needed in groups of closely related species. Identifying structures as staminodes is not always straightforward and sometimes requires corroborating phylogenetic and developmental evidence. Staminodial structures in Paronychia (Caryophyllaceae), for example, vary in shape and size and have been referred to as both petals and staminodes, rendering their homology uncertain. The development of staminodes was compared across …

The Distribution In Native Populations From Mexico And Central America Of The C677t Variant In The Mthfr Gene, Lucio A. Reyes

USF Tampa Graduate Theses and Dissertations

Objectives: To explore evolutionary hypotheses for the high frequencies of a substitution in the methylenetetrahydrofolate reductase (MTHFR) gene, in Mexican and Central American Indigenous populations.

Materials and methods: We obtained allele frequencies for the C677T variant in the MTHFR gene and ecological information for 37 indigenous samples from Mexico and Central America. We calculated Hardy–Weinberg equilibrium and computed Fst statistics. We computed correlations between the samples' allele frequencies and ecological and geochemical variables.

Results: Many of the samples have extremely high frequencies of the T allele (q̄ = 0.62, median = 0.66). In this region, the frequency of the T …

Epigenetic Memories And The Evolution Of Infectious Diseases, Geoff Wild

Biology Publications

Genes with identical DNA sequence may show differential expression because of epigenetic marks. Where epigenetic marks respond to past conditions, they represent a form of “memory”. Despite their medical relevance, the impact of memories on the evolution of infectious diseases has rarely been considered. Here we explore the evolution of virulence in pathogens that carry memories of the sex of their previous host. We show that this form of memory provides information about the sex of present and future hosts when the sexes differ in their pathogen’s transmission pattern. Memories of past hosts enable the evolution of greater virulence in …

Functional Genetic Approaches To Provide Evidence For The Role Of Toolkit Genes In The Evolution Of Complex Color Patterns In Drosophila Guttifera, Mujeeb Olushola Shittu

Dissertations, Master's Theses and Master's Reports

Toolkit genes are set of genes that orchestrate the development of basic body plan of animals, and they are highly conserved in all animals. The co-option of the toolkit genes into the pigmentation pathway has led to the evolution of novel species. This study focuses on understanding how the complex color patterns in animals develop by using the Drosophila species in the quinaria group as models. We developed an mRNA in situ hybridization (ISH) protocol, which allowed us to study gene expression patterns in the abdomen of developing pupae of non-model Drosophila species (Chapter 2). Through ISH, we found that …

The Evolution Of Technology, Kelly Cooper

Williams Honors College, Honors Research Projects

As per outlined by Dr. Quinn, this literature review will be a comprehensive review paper including an overview of current and previous research done in the field of Memetics. This will further include sifting through literature to hone in on a specific, new area of memetics Dr. Weeks is focused on, the evolutionary change of abiotic factors through purchasing. This is to be completed with the help of the library worshops designed to teach the skills necessary to undergo a literature review of this size. I will also participate in weekly reading groups to discuss papers and work closely with …

Within- And Trans-Generational Environmental Adaptation To Climate Change: Perspectives And New Challenges, Naim M. Bautista, Amélie Crespel

School of Biological Sciences: Faculty Publications

The current and projected impacts of climate change are shaped by unprecedented rates of change in environmental conditions. These changes likely mismatch the existing coping capacities of organisms within-generations and impose challenges for population resilience across generations. To better understand the impacts of projected scenarios of climate change on organismal fitness and population maintenance, it is crucial to consider and integrate the proximate sources of variability of plastic and adaptive responses to environmental change in future empirical approaches. Here we explore the implications of considering: (a) the variability in different time-scale events of climate change; (b) the variability in plastic …

Larval Anatomy Of Monotypic Painted Ant Nest Frogs Lithodytes Lineatus Reveals Putative Homoplasies With The Leptodactylus Pentadactylus Group (Anura: Leptodactylidae), Filipe A.C Do Nascimento, Rafael O. De Sá, Paulo C. De A. Garcia

Biology Faculty Publications

The morphological diversity of anuran larvae made them an important source of information for evolutionary and systematic studies. For the monotypic frog genus Lithodytes, which has an interesting taxonomic history, including its past synonymizing with Adenomera and its placement as a subgenus of Leptodactylus, the information provided from its larvae can help to understand its systematics interrelationships and also provide insights about its evolutionary trajectories. Herein, we provide a detailed description of the larval morphology of Lithodytes lineatus, including novel data of internal morphology (buccopharyngeal cavity and skeleton), and discuss some morphological and evolutionary aspects in relation …