Biology Commons^™

Novel Microbiological Medium Developed For The Isolation Of Bacteria Associated With Estuarine Anemones, Parker K. Lund, Catalina Cuellar-Gempeler

IdeaFest: Interdisciplinary Journal of Creative Works and Research from Cal Poly Humboldt

Out of the nearly one trillion species of microbiota estimated to inhabit Earth only ten thousand have been cultured in the laboratory. Culturing continues to play a vital role in determining the physiology and ecologic function of individual bacteria in microbial communities and for microbes associated with host organisms one of the major challenges is developing microbiological media that mimics the bacteria’s natural environment enough to promote growth. Here, we target bacteria associated with the estuary anemones Diadumene lineata and Metridium senile by developing a novel medium that uses anemone tissue as the sole source of nutrients. We further measured …

A Remote Sensing Approach To Assess The Historical Invasion Of Phragmites Australis In A Brackish Coastal Marsh, Jason S. Hagani, John Y. Takekawa, Steven C. Chappell, Richelle L. Tanner, Adrienne R. Ernst, Karin M. Kettenring

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Introduction: Coastal estuarine wetlands provide important habitats for a variety of endemic flora and fauna but are particularly vulnerable to biological invasions. Regular monitoring of changes in these vulnerable wetlands has become increasingly important for effective management, especially considering threats from climate change effects and human disturbance. Historical analyzes of plant invasions may guide targeted management strategies to eradicate harmful species. Estimating the distribution of invasive species has never been more accessible with the improved availability of high-resolution data and innovations in remote sensing, estimating the distribution of invasive species has never been more accessible.

Methods: We assessed the spread …

Using Podcasts To Bring National Estuarine Research Reserves Into The Classroom For Grades 6-12, Kaitlyn M. Dirr

Senior Theses

In a typical classroom setting, there are significant challenges to exposing students to concepts related to earth sciences and the environment. These challenges are exacerbated when conveying lessons about geographic areas with limited access, such as oceans and coastlines (Louv, 2010). It is now more important than ever for environmental education to improve and adapt to our changing world. Educators may have the opportunity to bring these subjects to life by using media content such as podcasts to introduce students to new places and the scientists, managers and educators that work in those spaces. In the United States, there exists …

Heavy Metal Distribution Across Three Georgia Estuaries Using Coastal Sharks As Sentinel Species, Allyson Stiles

Master of Science in Integrative Biology Theses

Estuaries are transitional environments that play key roles in coastal ecosystem functioning by providing essential habitats to ecologically important plant and animal species. Estuaries are exposed to a number of anthropogenic impacts, including heavy metal contamination from human activities. However, few studies have examined heavy metal distribution within Georgia’s estuaries and how intact salt marsh ecosystems could play a role in filtering and sequestering contaminants, such as Mercury (Hg). This thesis examined blood total mercury (THg) concentrations of four coastal shark species and concentrations of five heavy metals (Hg, As, Cr, Cu, and Pb) within the water and sediments of …

Nutrient Improvements In Chesapeake Bay: Direct Effect Of Load Reductions And Implications For Coastal Management, Rebecca R. Murphy, Jennifer Keisman, Jon Harcum, Renee R. Karrh, Mike Lane, Elgin S. Perry, Qian Zhang

Biological Sciences Faculty Publications

In Chesapeake Bay in the United States, decades of management efforts have resulted in modest reductions of nutrient loads from the watershed, but the corresponding improvements in estuarine water quality have not consistently followed. Generalized additive models were used to directly link river flows and nutrient loads from the watershed to nutrient trends in the estuary on a station-by-station basis, which allowed for identification of exactly when and where responses are happening. Results show that Chesapeake Bay’s total nitrogen and total phosphorus conditions are mostly improving after accounting for variation in freshwater flow. Almost all of these improving nutrient concentrations …

Effects Of Salinity On Eastern Oysters: Locating Lower-Salinity Tolerant Populations And Defining Resource Zones Suitable To Restoration, Fisheries, And Aquaculture., Lauren Swam

LSU Master's Theses

Eastern oysters (Crassostrea virginica) provide valuable ecosystem services and support a productive commercial industry in the northern Gulf of Mexico. Declining abundance from water quality changes and other factors drives development of management and restoration strategies focused on a comprehensive, metapopulation approach. Identifying oyster resource zones based on water quality combined with selective breeding of oysters adapted to specific conditions provides strategies to support aquaculture development and ensure resilient oyster populations and high production. Using 2015-2019 satellite-derived continuous salinity and temperature data for coastal Louisiana, this work created maps defining oyster resource zones supportive of (1) broodstock sanctuary …

Vignette 12: The Blob, Nicholas Bond

Institute Publications

A marine heat wave of unprecedented severity, areal extent and duration occurred in the Northeast Pacific Ocean during 2014-2016. This event, known as the “Blob,” had a wide variety of far- ranging effects on physical, chemical, and biological ocean properties. Because the Blob was such a massive perturbation, it represents an attractively large signal for inquiry in the Salish Sea. It represents a dress rehearsal for typical conditions in future decades due to global climate change.

Vignette 01: The Salish Sea Estuary System, Bert Webber

Institute Publications

The Salish Sea is an estuarine ecosystem. Freshwater from land drainages mixes with the waters of the Pacific Ocean and results in water with a measurable, although sometimes small amount of freshwater. The Salish Sea is among the preeminent estuaries of North America. Estuarine circulation and flow are central to the high biological productivity in the Salish Sea.

Vignette 03: Birds Of The Salish Sea, Rob Butler

Institute Publications

The significance of the Salish Sea comes into focus when we look at the diversity and abundance of its birds and mammals, some of which are globally, continentally, and nationally important. Of particular importance is the diversity and abundance of species on the Fraser River Delta. There are more species of birds on the delta than any comparable area in Canada, and nearly half of all 550 species of birds reported for British Columbia have been seen on the delta. Despite all that has been learned about marine birds and mammals, large areas of the Salish Sea in Canada have …

Vignette 07: Stormwater Effluent Exerts A Key Pressure On The Salish Sea, Emily Howe

Institute Publications

One of the primary terrestrial pressures on the Salish Sea estuarine and marine environment is urban stormwater runoff. When rainfall runs across hard, impervious surfaces, rather than soaking into the soil, it picks up and delivers toxic contaminants directly to nearby streams, rivers, and eventually the Salish Sea. In fact, for most toxic substances, surface runoff is the largest contributing source of loading to Puget Sound. Unfortunately, the Salish Sea’s relationship with stormwater effluent is no outlier; stormwater is the fastest growing cause of surface water impairment in the United States as urbanization transitions forested and other natural landscapes to …

Vignette 17: Salish Sea Jellyfish, Correigh Greene

Institute Publications

The Salish Sea is home to a diverse community of gelatinous zooplankton (or "jellies"). In their adult forms, jellies comprise a relatively large proportion of biomass in the Salish Sea. Questions regarding jellyfish abundance and climate variation in the Salish Sea have been difficult to address, in part because of a lack of consistent monitoring. Research presented in this vignette suggests that jellyfish are sensitive to climate signals like marine water temperatures, but do not appear to be systematically increasing in abundance over time. Due to advances in modeling, we may gain a better perspective on the roles jellies play …

Vignette 20: Fraser River Estuary In Need Of Urgent Intensive Care, Laura Kehoe, Tara G. Martin

Institute Publications

The Fraser River is the lifeline of the Salish Sea, influencing its stratification, circulation, and primary productivity. If we do not take strong action to conserve the Fraser River estuary, two-thirds of the species at risk in this region are predicted to have a less than 50% chance of survival. Many of the region's most iconic species could disappear. Conservation action combined with environmental governance is a pathway for a brighter future for the Fraser River and other highly contested regions.

Vignette 18: Bellingham Bay, Legacy Contamination Under Repair, Olivia Klein

Institute Publications

Bellingham Bay, home to twelve designated hazardous waste cleanup sites, illustrates the harm of past practices as well as the effectiveness of cleanup efforts. Since 2000, the Bellingham Bay cleanup has focused on the removal of contaminated sediment and soils introduced from a wide variety of sources, including construction and other industrial and municipal activities. Bellingham Bay cleanup is managed by the Washington State Department of Ecology (under the authority of Washington State’s Model Toxic Control Act) in coordination with a multi- agency Bellingham Bay Action Team.

Water Clarity At The River-Estuary Transition Zone: A Comparative Study Of The James, Mattaponi, And Pamunkey Sub-Estuaries, Rachel Henderson

Theses and Dissertations

Water clarity is a key parameter for monitoring water quality and often used to assess habitat suitability for submerged aquatic vegetation (SAV). Light attenuation, a measure of water clarity, is impacted by colored dissolved organic matter (CDOM), and by suspended particulates which include living and non-living components. We anticipated that the relative importance of these factors in regulating light attenuation would vary among the upper portions of three sub-estuaries differing in morphometry, hydrology, and degree of human influence. The James is characterized by eutrophic conditions and high algal abundance, whereas the Mattaponi and Pamunkey exhibit lower phytoplankton production. The Mattaponi …

Exploring The Effects Of Dam Removals On Zooplankton In Penobscot Estuary, Erin Bucci, Karen Wilson

Thinking Matters Symposium Archive

Estuaries provide many ecosystem services such as buffering the negative impacts of storms, offering recreation and commercial fishing opportunities, and they serve as a critical habitat corridor for migratory fish. In New England, estuarine habitats have been dramatically altered over the last 200 years due to dam construction; migratory fish such as Atlantic salmon and alewives have suffered. Recently, there have been several dam removal projects in Maine aimed to restore these populations – the largest of these efforts being the Penobscot River Restoration Project. In 2013, two dams were removed from the Penobscot River Estuary, opening more than 2,000 …

Seaweed Community Structure Along Environmental Gradients In An Ocean-Dominated Estuary, Saskia A. Raether

Cal Poly Humboldt theses and projects

Macrophyte community structure in estuaries that have been modified by the addition of hard substrata are poorly documented, especially in relation to the physical and environmental gradients that shape them. Therefore, this study describes the summer macrophyte flora that occurs on hard and soft substrata throughout Humboldt Bay, CA and tests how the macrophyte communities correlate to measures of horizontal and vertical environmental gradients. The percent cover of macroalgae, vascular plants, cyanobacteria, lichens, diatom film, sessile invertebrates, and substratum types were quantified during the summers of 2017 and 2018 at eleven intertidal locations in the bay and outer coast. Horizontal …

Environmental Conditions In San Martin Lake: Effects Of Eutrophication In An Estuary Associated With The Laguna Madre, Wendy L. Rogers

Theses and Dissertations

San Martin Lake is a shallow water Estuary in the lower Laguna Madre off the Port of Brownsville Shipping channel. It is influenced both by incoming tides and waste water runoff both from local farms and the Brownsville water treatment plants. The resulting mixture of eutrophic water and incoming sea water creates the perfect mix for a potential algal bloom. This study looks at the nutrient levels and environmental conditions in the main basin of the estuary to establish baseline levels and to observe the rate of nutrient clearing being accomplished in this environment. As sport recreation in the area …

Feeding Success Of Harbor Seals In Relation To Hunting Technique At Whatcom Creek, Mackenna Newmarch

Scholars Week

Factors that influence hunting success of seals and sea lions are underrepresented in studies of animal behavior. This is a critical interaction to understand when evaluating the top-down effects of pinnipeds on endangered Pacific salmon (Oncorhynchus spp.). In the Pacific Northwest, harbor seals (Phoca vitulina) consume a very large number of individual salmon, species that are a valuable resource and the subject of costly restorative efforts. A salmon hatchery in Whatcom Creek estuary of downtown Bellingham, Washington, attracts harbor seals that prey on returning adult Pacific salmon. The convenient location and small size of the site allows consistent observation of …

Assessment And Mapping Of The Milwaukee Estuary Habitat, Brennan Dow

Theses and Dissertations

Rivermouth regions such as the Milwaukee Harbor, are the habitat interface between watersheds and the Great Lakes proper, and can host a large diversity of fishes. To facilitate an ecosystem approach management strategy, I developed a layered map that includes bathymetry, side scan sonar images, shoreline substrate classifications, and initial data of aquatic vegetation and centrarchid spawning locations. The 60 km perimeter of the study area consisted of 59% hardened shoreline. Ground truthing of substrate classifications via Ponar grabs or video had about 95% accuracy for fine and rocky boulder substrates. Rocky fine was the most inaccurate classification (35%), but …

Detrital Protein Contributes To Oyster Nutrition And Growth In The Damariscotta Estuary, Maine, Usa, Cheyenne M. Adams

Electronic Theses and Dissertations

Oyster aquaculture is an expanding industry that relies on identifying and utilizing natural estuarine conditions for the economically viable production of a filter-feeding crop. The eastern oyster, Crassostrea virginica, is the principal species currently cultured in Maine. In addition to preferentially consumed phytoplankton, various detrital complexes (non-algal and/or non-living organic matter) may provide some nutrition to C. virginica between times of phytoplankton abundance. Here I investigated the importance of detrital proteins in supporting the growth of oysters cultured in the upper Damariscotta Estuary. Oyster aquaculture in this area is highly successful and previous reports indicate that labile detrital protein …

Tidal Creek And Substrate Effects On Oyster Reef Associated Fish And Decapod Condition And Density, Thomas S. Funk

Electronic Theses and Dissertations

Oyster reefs are essential fish habitat and a worldwide loss of reefs has the potential to negatively affect reef-associated nekton populations. Along the 100 km Myrtle Beach, SC shoreline, oyster reefs ostensibly have disappeared within swash tidal creeks, which are anthropogenically altered estuarine systems that drain into the coastal ocean directly over shoreline beaches. To address oyster reef losses, a series of shell bag reefs were constructed within multiple swash tidal creeks. Reefs also were constructed in tidal creeks associated with estuaries directly connected to the ocean by an inlet. The purpose of this study was to compare nekton usage …

Evaluation Of The Estrogenic And Osmoregulatory Impacts Of Exposure To 4-Nonylphenol Pollution In The Estuarine Arrow Goby, Clevelandia Ios, Kaitlin Marie Johnson

Master's Theses

Recent evidence indicates that some of California’s coastal estuaries are contaminated with the chemical 4-nonylphenol (4-NP). Tissue burdens of 4-NP detected in the intertidal arrow goby (Clevelandia ios) in California are among the highest recorded worldwide, however, it remains unknown whether these fish are impacted by this 4-NP contamination. 4-NP is an established endocrine disrupting compound with estrogenic properties that can alter reproductive function. Furthermore, evidence that estrogens can modulate iono- and osmo-regulatory function in fishes implies that estuarine fishes exposed to 4-NP may also experience an impaired ability to maintain hydromineral balance. In Chapter 1 of this …

Identifying Inundation-Driven Effects Among Intertidal Crassostrea Virginica In A Commercially Important Gulf Of Mexico Estuary, Joshua Solomon

Electronic Theses and Dissertations

Sea level rise and changing storm frequency and intensity resulting from climate change create tremendous amounts of uncertainty for coastal species. Intertidal species may be especially affected since they are dependent on daily inundation and exposure. The eastern oyster Crassostrea virginica is an economically and biologically important sessile intertidal species ranging from Canada to the Gulf of Mexico. Declines and changes in distribution of oyster populations has forced commercial harvesting to spread from subtidal to intertidal reefs. We investigated the potential responses of intertidal C. virginica to sea level rise, and the response of larval settlement to sedimentation which is …

Effects Of The Algal Toxin Microcystin On Fishes In The James River, Virginia, Maxwell D. Haase

Theses and Dissertations

With the global rise in frequency of harmful algal blooms in estuarine environments comes an increase in prevalence of toxic metabolites, such as microcystin (MC), that some of the cyanobacteria involved will produce. At high concentrations, MC may accumulate in consumer tissues and have deleterious effects on organisms; however impacts of the toxin on aquatic living resources at ecologically relevant concentrations have not been widely documented. We analyzed the effects of MC on juveniles of five fish species from the James River, Virginia to determine if MC has the potential to impede growth. Using three separate experimental approaches, it was …

Anthropogenic Disturbances In Estuarine Ecosystems: The Effects Of Altered Freshwater Inflow, Introduction Of Invasive Species, And Habitat Alteration In The Loxahatchee River, Fl, Zachary R. Jud

FIU Electronic Theses and Dissertations

With the majority of Earth’s population living in coastal areas, estuarine ecosystems have been particularly affected by anthropogenic disturbances. My dissertation research focused on three interrelated types of human disturbance that affect estuaries: Anthropogenic alteration of freshwater inflow, the introduction of invasive species, and habitat alteration. Using the LoxahatcheeRiver(Jupiter, FL) as a model system, my goal was to understand how these disturbances affect estuarine organisms, particularly fishes. One of the most ecologically harmful disturbances affecting estuaries is anthropogenic alteration of freshwater inflow (and resulting changes in salinity patterns). To identify effects of freshwater inflow on the behavior of an ecologically …

Jellyfish Identification And Quantification In The San Francisco Estuary, Amalia Borson, Lindsay L. Sullivan, Wim Kimmerer

STAR Program Research Presentations

As potential predators and competitors of plankton-eating fish, jellyfish have the potential to negatively impact fish populations. Jellyfish were collected weekly with plankton tows from the RombergTiburonCenterpier in Tiburon, CA. Since some jellyfish were too small to identify, one tow was collected and preserved to record abundances, and a second tow was collected to rear jellyfish until distinguishing characteristics were visible enough for identification. Jellyfish in the preserved tows were then identified, measured, and counted, and their abundance (number m^-3) was calculated. Jellyfish from the second tows were reared in plastic buckets that were lightly bubbled using aquarium …

Tidal Influences On Bacterial And Phytoplankton Abundances And The Resulting Effects On Patterns Of Dissolved Oxygen In The Skidaway River Estuary, Casey Colleen Brazell

Electronic Theses and Dissertations

Two studies were conducted to investigate the physical and biological processes contributing to the dissolved oxygen (DO) availability in the Skidaway River Estuary (SRE), Savannah, GA during the summer of 2005. A temporal study looked at changes in DO concentrations, Chlorophyll a concentrations, bacterial abundance, water depth, and salinity, every hour, for 26 hours, over both a neap and a spring tide. A spatial study looked at changes in the above variables at 5 sites along the SRE while following the tide inland during a neap high and low tide, and a spring high and low tide. DO concentrations varied …

Applying Marine Protected Area Design Models In Large Estuarine Systems, Roman Zajac, Amanda E. Neely

Biology and Environmental Science Faculty Publications

Several types of design models are currently being used to develop marine protected areas (MPAs) for conservation of coastal and pelagic ecosystems. However, few studies have applied these models in large estuaries which have unique characteristics that need to be considered in MPA design, including strong physical, chemical and biological gradients and significant human impacts. We explored how one design model, MARXAN, can be applied to estuarine systems by developing MPA design scenarios for Long Island Sound, an estuary in the northeastern USA. Using sedimentary texture as a proxy for habitats, we modeled and tested several scenarios where conservation goals …

Microalgal-Meiofaunal Trophic Relationships In Muddy Intertidal Estuarine Sediments, James L. Pinckney, Kevin R. Carman, S. Elizabeth Lumsden, Sabrina N. Hymel

Faculty Publications

The determination of the primary factors controlling carbon (C) transfer rates in situ are a basic requirement for developing a mechanistic view of the processes that regulate benthic microbial community structure and function. In many estuarine ecosystems, the base of the food web is dominated by microalgal production; however, information on the quantitative relationships and process rates of meiofaunal grazers and benthic microalgal (BMA) producers is limited to a few habitats and short temporal scales. The primary objective of this study was to examine the trophic interactions between meiofaunal grazers and BMA producers to determine if grazing is a primary …

Responses Of Phytoplankton And Pfiesteria-Like Dinoflagellate Zoospores To Nutrient Enrichment In The Neuse River Estuary, North Carolina, Usa, James L. Pinckney, Hans W. Paerl, Elin Haugen, Patricia A. Tester

Faculty Publications

The recently described toxic dinoflagellate Pfiestena piscicida and morphologically similar Pfiesteria-like dinoflagellates have become a major water quality issue with possible fish mortality and reported human health implications. The linkages between accelerated nutrient loading, eutrophication, and the proliferation of this group of dinoflagellates, however, are not well established for natural Systems. Phytoplankton pnmary production may provide a key link between nutrient inputs and potential outbreaks of Pfiesteria-like biflagellated zoospores in the Neuse River Estuary, North Carolina. The impacts of nutrient (NO₃^- -nitrogen and PO₄^3- -phosphorus) supply rates, sediment-water column exchange, water column mixing, and phytoplankton prey …